Expression of miR-373 and its predicted target genes E-cadherin and CD44 in patients with laryngeal squamous cell carcinoma.
Authors: Timirci-Kahraman Ö, Verim A, Farooqi AA, Turan S, Özkan-Küçük NE, Yaylim İ Abstract Laryngeal squamous cell carcinoma (LSCC) is a genomically complex disease that is difficult to target, and efforts have been made to identify new treatment strategies and   molecular markers that might stratify patients and individualize options for treatment. miR-373 has diametrically opposed roles in different stages and types of cancers. miR-373 has been suggested to quantitatively control E-cadherin and CD44 expression. We studied the expression of miR-373, E-cadherin and CD44 in la...
Source: Cellular and Molecular Biology - January 10, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Protective effect of gel form of gastric gavage applicated aloe vera on ischemia reperfusion injury in renal and lung tissue.
This study aimed to research the effect of aloe vera to prevent renal and lung tissue damage in an experimental ischemia-reperfusion (I/R) injury model. The study included 21 male Wistar Albino rats, which were categorized into control group, n = 7 (no procedures), Sham group n = 7 (I/R); and aloe vera therapy group, n = 7 (aloe vera and I/R). Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and malondialdehyde (MDA) were evaluated from lung and kidney tissues for biochemical investigations. As histopathological, hematoxylin and eosin and anti-iNOS were also examined. In biochemical investigations,...
Source: Cellular and Molecular Biology - January 10, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The effects of apomorphine on paracetamol-induced hepatotoxicity in rats.
Authors: Sehitoglu MH, Yayla M, Kiraz A, Oztopuz RO, Bayir Y, Karaca T, Khalid S, Akpinar E Abstract It is becoming progressively more understandable that overdose of paracetamol in both humans and animals causes severe hepatotoxicity. Apomorphine is known as a neuroprotective agent. Due to the protective effect,  apomorphine had been tested in experimental studies on different models. Findings obtained through series of expriments suggested that apomorphine may also be useful in liver toxicity. The aim of this study is to investigate the relationship among the hepatoprotective mechanism of apomorphine and to ...
Source: Cellular and Molecular Biology - January 10, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Evaluation of STAT3 rs1053004 single nucleotide polymorphism in patients with chronic hepatitis B and hepatocellular carcinoma.
Authors: Fatemipour M, Arabzadeh SAM, Molaei H, Geramizadeh B, Dabiri S, Fatemipour B, Vahedi SM, Malekpour Afshar R Abstract Chronic infection with hepatitis B (CHB) virus is one of the most important risk factors for Hepatocellular carcinoma (HCC). Hepatitis B virus (HBV) causes liver cancer in various ways. One of these ways is increasing the expression of the signal transducer and activator of transcription 3 (STAT3) in Hepatocytes by HBV. The purpose of this research is to evaluate the single nucleotide polymorphism (SNP) rs1053004 in the STAT3 gene in CHB patients and individuals who suffer from HCC. In this ...
Source: Cellular and Molecular Biology - January 10, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Evaluation of protein levels of autophagy markers (Beclin 1 and SQSTM1/p62) and phosphorylation of cyclin E in the placenta of women with preeclampsia.
Authors: Akcora Yildiz D, Irtegun Kandemir S, Agacayak E, Deveci E Abstract Preeclampsia is a severe multisystem disorder, and its pathophysiology is still not completely understood. Autophagy, a recycling process that maintains cellular homoeostasis during differentiation and development, is controversial regarding increased or decreased autophagic activity in preeclampsia. The aim of this study was to determine whether autophagy is increased in the placentas of women with preeclampsia by examining the protein levels of autophagy markers (Beclin 1 and SQSTM1/p62) and phosphorylation of cyclin E. For this purpose, ...
Source: Cellular and Molecular Biology - January 10, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Hepatoprotective properties for Salvia cryptantha extract on carbon tetrachloride-induced liver injury.
Authors: Yalcin A, Yumrutas O, Kuloglu T, Elibol E, Parlar A, Yilmaz İ, Pehlivan M, Dogukan M, Uckardes F, Aydin H, Turk A, Uludag O, Sahin İ, Ugur K, Aydin S Abstract The present study was designed to determine the possible hepatoprotective effects of Salvia cryptantha (black weed) plant extract against carbon tetrachloride (CCl4)-induced hepatic injury in rats. Animals were grouped as follows: control group (Group I), CCl4 group (Group II), olive oil group (Group III), CCl4 + S. cryphantha 200 mg/kg group (Group IV), and CCl4 + S. cryptantha 400mg/kg group (Group V). Rats...
Source: Cellular and Molecular Biology - January 10, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Molecular and phenotypic characterization of the vancomycin-resistant gene in bacterial isolates acquired from catheter tips.
Authors: Siddiqui S, Saeed M, Faisal SM, Akhtar A, Sarim KM, Khan M, Ahmad I, Ahmad I Abstract Gram-positive bacteria, particularly Staphylococcus aureus is a significant pathogen, not only in the hospital setting but the community also. S. aureus is a major cause of serious hospital and community-acquired infections, particularly in the colonized individuals. The emergence of vancomycin-resistant S. aureus (VRSA) strains has led to global concerns about treatments for staphylococcal infections. Until now, few strains of VRSA have been reported worldwide. The conventional disk diffusion method for determination of ...
Source: Cellular and Molecular Biology - January 10, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Regulation of signal transduction cascades by Pterostilbenes in different cancers: Is it a death knell for oncogenic pathways.
Authors: Butt G, Attar R, Tabassum S, Aras A, Qadir MI, Ozbey U, Alaaeddine N, Ozcelik B, Farooqi AA Abstract Interdisciplinary research has revolutionized the field of medicine and we have witnessed exponential increase in the high-impact research in past few decades. However, the road to this burgeoning research field is obstacle-ridden because of intratumor heterogeneity, loss of apoptosis and dysregulation of spatio-temporally controlled signaling pathways. Ground-breaking findings obtained through genetic, genomic and proteomic studies have considerably improved our concepts related to the complexity of protei...
Source: Cellular and Molecular Biology - January 10, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Astemizole inhibits cell proliferation in human prostate tumorigenic cells expressing ether à-go-go-1 potassium channels.
Astemizole inhibits cell proliferation in human prostate tumorigenic cells expressing ether à-go-go-1 potassium channels. Cell Mol Biol (Noisy-le-grand). 2017 Dec 17;63(12):11-13 Authors: Bernal-Ramos G, Hernández-Gallegos E, Vera E, Chávez-López MG, Zúñiga-García V, Sánchez-Pérez Y, Garrido E, Camacho J Abstract Prostate cancer (PC) is the main cause of cancer mortality in men worldwide. Therefore, novel treatments for PC are needed. Ether à-go-go-1 (Eag1) potassium channels display oncogenic properties, and have been suggested as e...
Source: Cellular and Molecular Biology - January 10, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Nanobiotechnology: A promising scope of gold biotechnology.
Authors: Barabadi H PMID: 29307347 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - January 10, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Biochemical evaluation of hydroxyurea derivative schiff bases in liver of rats.
In conclusion, it can be suggested that the determination of the pharmacological characteristics of them can be beneficial in numerous fields of application thanks to the antioxidant and hepatotoxic activities demonstrated by hydroxyurea derivative Schiff bases. PMID: 29208168 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - December 8, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effect of melatonin on cytokine levels in a hyperthermia-induced febrile seizure model.
In this study, we investigated serum cytokine levels in a hyperthermia-induced febrile rat seizure model and the effect of melatonin. A total of 28 male Sprague-Dawley rats were divided into four groups: the control (C) group, healthy melatonin (MT) group, and hyperthermia-induced febrile seizure groups with (HIFS-MT) and without (HIFS) administration of melatonin. Melatonin (80 mg/kg) was given intraperitoneally 15 min before the seizure. HIFS was induced by placing the rats in 45°C water. The rats were sacrificed under anesthesia after the seizure. Blood samples were drawn by transcardiac puncture to measure serum cy...
Source: Cellular and Molecular Biology - December 8, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Ferula gummosa gum induces apoptosis via ROS mechanism in human leukemic cells.
In conclusion, the present study demonstrated that F. gummosa induced apoptosis through ROS mechanism on leukemic cells as a concentration and time dependent manner. The precise signaling pathway by which F. gummosa induce apoptosis needs further research. PMID: 29208170 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - December 8, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Ecological and biological studies on five-lined skink, Trachylepis (= Mabuya) quinquetaeniata inhabiting two different habitats in Egypt.
This study was carried out to study the diet type, genetic, morphometric and histological variations between two natural populations of Trachylepis quinquetaeniata inhabiting Abu-Rawash, Embaba, North Giza and Garfas-Senoures, El-Faiyum habitats. In this study, morphometric examination showed that Trachylepis quinquetaeniata is a typically ground-dwelling Skink and seems to be frequently associated with grass and below shrubs. Geographic variation in sexual size dimorphism (SSD) and head size dimorphism (HSD) with greater dimensions in Garfas-Senoures, El-Faiyum compared with Abu-Rawash, Embaba, North Giza might be correla...
Source: Cellular and Molecular Biology - December 8, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Investigation of role of vascular endothelial growth factor, Annexin A5 and Apelin by immunohistochemistry method in placenta of preeclampsia patients.
In this study, placentas were collected from 20 normotensive pregnant women as controls, 16 mild-preeclamptic pregnant women, and 16 severe preeclamptic women. VEGF, Annexin A5 and Apelin were examined in samples of placenta tissues by streptavidin-biotin-peroxidase complex immunohistochemical methods. Immunoreactivity scores (IRS) were obtained for each parameter.  VEGF and Apelin IRS were increased significantly in preeclamptic groups compared with control group (p <0.026, p<0.002 respectively). But Annexin A5 IRS was decreased significantly in preeclamptic groups compared with control group (p<...
Source: Cellular and Molecular Biology - December 8, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Transforming Growth Factor- β1 gene polymorphism and osteoporosis in postmenopausal egyptian women.
This study was performed on 138 postmenopausal osteoporosis/osteopenic women and 128 postmenopausal female control group. There was a significant statistical difference in the CC, CT and TT (T869C) genotype frequencies between the osteopenia/osteoporosis and control subjects (p value <0.001). There was a non-significant statistical difference in the CC, CT and TT (T-509C) genotype frequencies between the osteopenia/osteoporosis and control subjects (p value <0.082). There was a significant statistical difference between TT,CT and CC of (T869C) and T score, Z score and calcium of osteopenia/osteoporosis group ...
Source: Cellular and Molecular Biology - December 8, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Strong antimicrobial activity of Hypericum perforatum L. against oral isolates of Lactobacillus spp.
Authors: Khadem Nezhad S, Taghavi Zenouz A, Aghazadeh M, Samadi Kafil H Abstract Lactobacillus spp. are one of the first microorganisms involved in the development of dental caries in the first years of life of the child. The purpose of this study was to investigate the antibacterial effect of alcoholic extract of hypericin against strains of Lactobacillus spp. and determine its related MIC (minimum inhibitory concentration); and cytotoxic effect against gingival fibroblasts. Antimicrobial activity and MIC were evaluated using micro broth dilution method based on CLSI (clinical and laboratory standards institute) p...
Source: Cellular and Molecular Biology - December 8, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Antifungal activity of silver nanoparticles on Fluconazole resistant Dermatophytes identified by (GACA)4 and isolated from primary school children suffering from Tinea Capitis in Ismailia - Egypt.
This study is aimed to detect the antifungal activity of silver nanoparticles (SNPs) on Fluconazole resistant dermatophytes isolated from primary school children clinically suffering from tinea capitis and attending El-Sheikh Zaid Dermatology Center in Ismailia. The study was done on 112 clinical cases. Examination with potassium hydroxide(KOH) of hair samples was done, followed by routine identification using culturing, macroscopical and microscopical examination and biochemical tests, finally molecular identification using Polymerase Chain Reaction (PCR) with (GACA) 4 was done. Fluconazole resistance of these dermatophyt...
Source: Cellular and Molecular Biology - December 8, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Analysis of potential interactions between vitamins D and C using gene expression profiles from mouse models.
This study is to investigate how VD and VC interact at molecular level using whole genome expression profiles from spleens of 111 mouse strains and livers from 40 mouse strains. We first identified the genes that are closely correlated to vitamin D receptor (Vdr) and gulonolactone oxidase (Lgo). We next analyzed the potential molecular pathways of Vdrand Lgo correlated genes and examined how these two sets of pathways are connected in liver and spleen. Our results indicated that Vdrand Lgo associate to distinct groups of genes. While most of genes are not the same, a few of them are associated to both Vdrand Lgo.  Our...
Source: Cellular and Molecular Biology - December 8, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Human haematological and epithelial tumor-derived cell lines express distinct patterns of onco-microRNAs.
In this study, we analysed differences in miRNA expression profile between haematological and epithelial tumor-derived cell lines and explored their role in definying different cancer cells phenotypes. Cancer Focus microRNA PCR Panel was used to analyze eighty-four oncomiRNAs in two human haematological (K562 and HL-60) and in two epithelial (H460 and MCF-7) cancer cell lines. Bioinformatic tools were used to identify miRNA-specific signatures and to discover potentially deregulated pathways. Our analysis led to the identification of i) a large repertoire of miRNAs commonly expressed in the four cell lines, including two e...
Source: Cellular and Molecular Biology - December 8, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Bioactive flavones isolated from Tunisian Artemisia campestris L. Leaves.
Authors: Metoui R, Bouajila J, Znati M, Cazaux S, Neffati M, Akrout A Abstract Four flavones were isolated from dried leaves of Artemisia campestris L. 2',4',5,7-tetrahydroxy-5',6-dimethoxyflavone, eupatilin and dimethoxycentaureidin were reported for the first time in this species whereas cirsiliol was previously identified but it was isolated for the first time. Their structures were elucidated by spectroscopic methods, including 1D and 2D NMR experiments and mass spectrometry analysis. In addition, all isolated flavones were evaluated for their antioxidant, anti-inflammatory, anti-superoxide dismutase, anti-xant...
Source: Cellular and Molecular Biology - December 8, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Genetic characterization and assessment of demographic bottleneck in Caspian horse population.
This study determined the efficiency of microsatellite markers for conservation plans and breeding strategies in Caspian horse population. For this purpose, 120 Caspian horse samples including 95 adults and 25 foals were genotyped by using seventeen microsatellite markers recommended by ISAG. The number of allele per locus varied from 5 (HMS01 and HTG07) to 9 (HTG10) with an average value of 7.41. The observed heterozygosity and the expected heterozygosity ranged 0.505-0.831 (mean 0.684), from 0.615-0.835 (mean 0.748) respectively. PIC value ranged from 0.716 (HMS01) to 0.834 (AHT04) with average 0.787. The total exclusion...
Source: Cellular and Molecular Biology - December 8, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Curcumin induce DNA damage and apoptosis through generation of reactive oxygen species and reducing mitochondrial membrane potential in melanoma cancer cells.
Authors: Kocyigit A, Guler EM Abstract Melanoma is the most malignant skin cancer. Curcumin has shown to have therapeutic effects when used in the treatment of malignant diseases. However, the precise molecular mechanisms of its action are not fully elucidated. In this research, we hypothesized that reactive oxygen species (ROS) play a key role in curcumin induced DNA damage, apoptosis and cell dead. To test our hypothesis, cytotoxic, genotoxic, apoptotic, ROS generating and mitochondrial membrane potential (MMP) of curcumin on mouse melanoma cancer cells (B16-F10) and fibroblastic normal cells (L-929) were in...
Source: Cellular and Molecular Biology - December 8, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Potential biomarkers for the diagnosis of respiratory tract infection and lungs cancer.
Authors: Khan K, Aslam MA, Zahra FT, Basheer H, Bilal M, Sumrin A Abstract Major hurdle faced by many physicians in treating various respiratory tract infections and lung carcinomas is their late or mis-diagnosis. In most respiratory tract infections the manner of infection is not completely understood. Similarly, various lung carcinomas are diagnosed at advance stages at which not only the treatment possibilities are narrowed but the chances of survival are also reduced. So, for the sake of better treatment, the quick and improved diagnostic strategies are suggested. Protein biomarkers fully fit the description in...
Source: Cellular and Molecular Biology - December 8, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Complexity of phenotypes induced by p.Asn1303Lys-CFTR correlates with difficulty to rescue and activate this protein.
Authors: Farhat R, El-Seedy A, Norez C, Talbot H, Pasquet MC, Adolphe C, Kitzis A, Ladevèze V Abstract Cystic Fibrosis is the most common recessive autosomal rare disease found in Caucasian. It is caused by mutations on the Cystic Fibrosis Transmembrane Conductance Regulator gene (CFTR) that encodes for a protein located on the apical membrane of epithelial cells. c.3909C>G (p.Asn1303Lys) is one of the most common worldwide mutations located in nucleotide binding domain 2. The effect of the p.Asn1303Lys mutation on misprocessing was studied by immunofluorescence and western blotting analysis in presen...
Source: Cellular and Molecular Biology - December 8, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Promoter motifs required for c-mpl gene expression induced by thrombopoietin in CMK cells.
Authors: Sunohara M, Sato I, Morikawa S Abstract Thrombopoietin (TPO) and its receptor, c-Mpl, are the central regulators of megakaryocyte development and platelet production and are also crucial to regulate megakaryocytopoiesis. TPO remarkably elevated c-mpl promoter activity, while the protein kinase C (PKC) inhibitors, GF109203, H7 and Calphostin C, clearly reduced the steady level of its promoter activity.  In the present study, motifs crucial for c-mpl promoter activity induced by TPO treatment has been analyzed using a human megakaryoblastic cell line, CMK. Destruction of the -107Sp1 and the -57Sp1 sites...
Source: Cellular and Molecular Biology - December 8, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Development of PCR primer systems for amplification of 16S-rDNA to detect of Thiobacillus spp.
Authors: Beheshti Ale Agha A, Kahrizi D, Ahmadvand A, Bashiri H, Fakhri R Abstract Thiobacillus is a genus of Gram-negative, rod-shaped and autotrophic Betaproteobacteria. They catalyze the dissimilatory oxidation of elemental sulfur and reduced inorganic sulfur compounds. Whereas more than 30 species have been known in this genus, most were never reliably or effectively published. The rest were either reclassified into Thiomonas, Paracoccus, Starkeya, Sulfuriferula, Halothiobacillus, Thermithiobacillus or Acidithiobacillus, were lost from culture. Most of Thiobacillus species are obligate autotrophs via elementary...
Source: Cellular and Molecular Biology - December 8, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Association between vitamin D receptor BsmI gene polymorphism and periodontitis: a meta-analysis in a single ethnic group.
In conclusion, our meta-analysis showed that VDR BsmI polymorphism was associated with the decreased risk of periodontitis in Chinese individuals from South China, and further studies in other ethic groups are required for definite conclusions. PMID: 29208185 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - December 8, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Association between platelet glycoprotein Ia C807T gene polymorphism and ischemic stroke: a meta-analysis in a separate ethnic group.
Authors: Huang XY, Fu WJ, Mei ZZ, Yu YL, Huang YH, Lin H, Chen JJ, Wang MX, Guan SB, Fang HW Abstract Many studies have been examined the association of platelet glycoprotein (GP) Ia C807T polymorphism with ischemic stroke (IS) susceptibility. However, the results of these studies are inconsistent. To further assess the effects of GP Ia C807T polymorphism on the risk of IS, a meta-analysis was performed in a separate ethnic group. Relevant studies were identified using PubMed and Chinese databases through January 2017. Pooled odds ratios (ORs) and 95% confidence intervals (CIs) were used to assess the strength of t...
Source: Cellular and Molecular Biology - December 8, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

A meta-analysis study of the association between EGFR rs2252586 mutation and the risk of glioma.
In conclusion, this meta-analysis found that EGFR rs2252586 mutation was significantly associated with glioma risk. PMID: 29208187 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - December 8, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The antiosteoporosis effect of icariin in ovariectomized rats: a systematic review and meta-analysis.
Authors: Liu Y, Zuo H, Liu X, Xiong J, Pei X Abstract Herba Epimedii (Yinyanghuo or Horny Goat Weed) is a traditional Chinese medicine widely used in treating osteoporosis. As the major active component, icariin is intensively investigated in the prevention and treatment of osteoporosis in ovariectomized rats. However, misleading conclusions can be generated in animal studies with various experimental designs. Therefore, a systematic review and meta-analysis was performed to evaluate the efficacy of icariin against osteoporosis in ovariectomized rats. PubMed, EMBASE and Chinese National Knowledge Infrastructure dat...
Source: Cellular and Molecular Biology - December 8, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Enlightenment and lesson from the successful treatment of acute superior mesenteric artery embolism.
Authors: Wang B, Sun R, Chen W, Li T Abstract  Despite recent advances, treatment of acute superior mesenteric artery embolism (SMAE) in emergency is still very difficult. The comparative rarity, the difficulty of early diagnosis, and the extremely high mortality of SMAE give us the sufficient reasons to report our successful experience in curing a patient with acute SMAE. In the present case, the patient was diagnosed early without evidence of intestinal necrosis. The diagnosis of SMAE was verified by computed tomography angiography (CTA). A comprehensive treatment was immediately employed including a combina...
Source: Cellular and Molecular Biology - December 8, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Metabolic and molecular responses to calcium soap of fish oil fed to ewes during peripartal period.
Authors: Sheibani S, Khadem AA, Mohammadi-Sangcheshmeh A, Alamouti AA, Norouzian MA Abstract It has been shown that n-3 long chain fatty acids (n-3 LCFA) are involved in energy/lipid metabolisms, reproductive parameters, and molecular regulations leading to maintained homeostasis. We hypothesized that supplementation of peripartal diets with fish oil (FO), as a source of n-3 LCFA, could improve energy balance and modulate metabolic pressure in a sheep model. Prepartum ewes (n = 24) were fed control (CON) or  calcium soap of fish oil (FO) supplemented-diet from four weeks before until three weeks after parturat...
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Induced dedifferentiation of barley (Hordeum vulgare L.) embryonic cells and its relationship with agronomic traits.
This study was carried out to investigate the response of 42 Iranian and European barley (Hordeum vulgare L.) cultivars to induced dedifferentiation of embryonic cells via immature embryo culture and understand the relationship between embryo culture characters and agronomic traits. The cultivars were evaluated for dedifferentiation of embryonic cells or callus induction from immature embryo culture based on a completely randomized design with unequal replication. Immature embryos were placed scutellum down on cell dedifferentiation medium based on MS and supplemented with 2.5 mg/l 2,4-D. The developed calli were transferr...
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effect of pomegranate (Punica granatum L.) juice on kidney, liver, heart and testis histopathological changes, and the tissues lipid peroxidation and antioxidant status in lead acetate-treated rats.
This study investigated the protective potential of PJ against lead-induced oxidative stress. A total of forty adult male Sprague Dawley rats were divided into four experimental groups. The animals were fed a standard pellet diet and tap water ad libitum. The rats were divided into four groups (n=10 for each group): control, lead asetat (2000 ppm), low-treated PJ- a daily dose of 2.000 ppm lead plus 30µl pomegranate juice (included 1.050 µmol total polyphenols, gallic acid equivalent), and high-treated PJ- a daily dose of 2.000 ppm lead plus 60µl pomegranate juice (included 2.100 µmol total polyphen...
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

HMGCR and ApoE mutations may cause different responses to lipid lowering statin therapy.
The objective of this study was to investigate whether common variations in HMG-CoA Reductase(HMGCR) and Apolipoprotein E (ApoE) genes involved in lipid and statin metabolism modify the effect of statins on serum lipid and lipoprotein concentrations in CAD patients.A hundred  CAD patients were enrolled into the study. At the beginning of the study biochemical measurements were performed to determine the baseline levels performed. Patients were treated with 40 mg atorvastatin for 2 months and biochemical measurements were repeated. According to the post-treatment, LDL-c levels,  patients were divided into 2 groups...
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Cardioprotective effect of Pycnogenol in ischemic-reperfusion injury (IRI) in rats.
Authors: Xiao P, Zhang K, Tao Z, Liu N, Ge B Abstract Oxidative stress plays a critical task in the biochemical and pathological alteration linked with myocardial ischemic-reperfusion injury (IRI). This warrants identifying agents with a potential for preventing such damage in an effective way. A novel plant based product, Pycnogenol, obtained from the French maritime pine (Pinus pinaster ssp. atlantica) bark extract was known for its tremendous antioxidant potential (both in vivo, in vitro). It was able to attenuate the symptoms of immune dysfunction through restoring a cellular antioxidant status in low micronutr...
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Hyperandrogenism stimulates inflammation and promote apoptosis of cumulus cells.
Authors: Xu F, Liu R, Cao X Abstract To explore roles of androgen and cytokines such as IL-1, IL-6 and TNF-α in PCOS. Cumulus cells were isolated from rat COCs and divided into two sets of experiments. Set 1 includes 4 groups: Control group, IL-1 group (final concentration at 5 nM), IL-6 group (final concentration at 5nM) and TNF-α group (final concentration at 5nM). Set 2 also include 4 groups: Control group, Androgen group 1 (final concentration at 1 nM), Androgen group 2 (final concentration at 10 nM) and Androgen group 3 (final concentration at 100 nM). Androgen, at final concentration of 100 nM, ca...
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Application of unilateral multiple channels approach in percutaneous vertebroplasty for osteoporotic vertebral fractures.
This study aimed to investigate the application valuable of percutaneous vertebroplasty (PVP) with unilateral multiple channels approach in osteoporotic vertebral fractures patients. A retrospective review was conducted on 685 consecutive patients with osteoporotic vertebral fracture from March 2003 to October 2012. Among them, 82 cases were given PVP procedure by unilateral multiple channels approach. The timing of surgery, bone cements injection, and complications were analyzed. By using the X-ray film, the distribution of cement was detected and scored. The visual analogue scale (VAS) score and Oswestry disability index...
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Overexpression of microRNA-133b reduces myocardial injuries in children with viral myocarditis by targeting Rab27B gene.
Authors: Zhang Y, Sun L, Sun H, Liu X, Luo X, Li C, Sun D, Li T Abstract The present study is to measure the expression of microRNA (miRNA or miR)-133b in circulating blood of children with viral myocarditis before and after drug treatment, and to investigate its relationship with the severity of myocardial lesions. A total of 36 children patients with viral myocarditis who received treatments at our hospital between June 2014 and June 2016 were enrolled in the present study, including 21 boys and 15 girls (age range, 9 months - 16 years).Quantitative real-time polymerase chain reaction was used to determine the ex...
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Investigation of genotoxic effects of doripenem using cytogenetic and molecular methods.
In this study, according to the obtained results, it was determined that DRP failed to show any genotoxic risk at the therapeutic doses. This result also indicates that DRP could be a reliable antibiotics according to its rapid metabolism. PMID: 29096747 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Organic chromium modifies the expression of orexin and glucose transporters of ovarian in heat-stressed laying hens.
In conclusion, CrPic and CrHis showed supported the relief and treatment of stress complications. PMID: 29096748 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

A bioinformatics approach for identification of miR-100 targets implicated in breast cancer.
Authors: Shamsi R, Seifi-Alan M, Behmanesh A, Omrani MD, Mirfakhraie R, Ghafouri-Fard S Abstract MicroRNAs (miRNAs) are small endogenous non-coding RNAs with principal roles in regulation of protein expression via translation repression and mRNA degradation. Based on these roles they are implicated in tumourigenesis processes as well. Among them is miR-100 which can exert both tumor suppressor and oncogenic functions in various cancer types. In breast cancer, it has been shown to affect apoptosis, epithelial-mesenchymal transition as well as tumor-related signaling pathways. In the present study, we introduce a nov...
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Clinical efficacy and mechanism of lymphoplasma exchange in the treatment of Guillain-Barre syndrome.
Authors: Luo MC, Wang WF, Yin WF, Li Y, Li BJ, Duan WW, Zeng QM, Luo YB, Yang XS, Yang H Abstract Guillain-Barre syndrome (GBS) is an autoimmune disease of the nervous system and is the most common acute polyneuropathy. Both cellular and humoral immunity are believed to be involved in the pathogenesis of GBS, and various types of activated CD4+ T cells are thought to orchestrate the onset and progression of GBS. Lymphoplasma exchange (LPE) filtering out activated lymphocytes while exchanging plasma has been used for GBS treatment for years. However the treatment is still not yet optimal. In order to assess the effi...
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

LPS-induced Src family kinases activity mediates IL-10 production through activation of STAT3 in peripheral blood mononuclear cells of patients with Beh çet's Disease.
LPS-induced Src family kinases activity mediates IL-10 production through activation of STAT3 in peripheral blood mononuclear cells of patients with Behçet's Disease. Cell Mol Biol (Noisy-le-grand). 2017 Oct 31;63(10):116-121 Authors: Irtegun Kandemir S, Tekin MA, Bozkurt M, Dagli AZ, Kalkanli-Tas S Abstract Behçet's disease (BD) is achronic inflammatory disorder characterized by recurrent oral and genital ulcers, uveitis and skin lesions. Although,the pathogenesis of BD remains poorly understood, excessive or dysregulatedcytokine production including IL-10 is associated with BD. Revealin...
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The association between murine double minute 2 (MDM2) rs2279744 and endometrial cancer risk in a Chinese Han population.
In conclusion, the study found that MDM2 rs2279744 polymorphism was significantly associated with endometrial cancer risk in a Chinese Han population. PMID: 29096752 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Interleukin-6 secreted by oral cancer- associated fibroblast accelerated VEGF expression in tumor and stroma cells.
In this study, we examined the correlation between IL-6 and CAF and the role of this correlation on VEGF production. In this study, quantitative expression level of IL-6 and VEGF in CAF and Oral Cancer Cells (OCCs) examined through Real Time PCR and ELISA and western blot analysis. In addition, maintenance and retention of IL-6 and VEGF checked out in co-culture experiment of CAF and OCC cells. These experiments demonstrated that in oral cancer, CAF cell line secretes significantly more IL-6 than OCC. Also IL-6 is a factor that causes VEGF secretion in CAF cell line. CAF is the basic and the most essential source for produ...
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Overview of ribosomal and non-ribosomal antimicrobial peptides produced by Gram positive bacteria.
Authors: Tajbakhsh M, Karimi A, Fallah F, Akhavan MM Abstract The increasing incidence of antimicrobial resistance bacterial infection and decreasing effectiveness of conventional antibiotics to treatment have caused serious problems worldwide. The demand for new generationantibiotics to combat microbial pathogens is imperative. Cationic antimicrobial peptides (AMPs) with different sources from prokaryotic to complex eukaryotic organisms, with variable length, amino acid composition and secondary structure, have been consideredduring the past decades.  The advantages of large number of AMPs are related to broa...
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Ziziphus Jujube: a review study of its anticancer effects in various tumor models invitro and invivo.
Authors: Ebrahimi S, Mollaei H, Hoshyar R Abstract The growing rate of cancer incidences and inefficiency of current therapies have made scientists to find novel agents such as medicinal plants. Ziziphus jujube is one of these plants whose pharmaceutical effects have been studied for a long time. Recent investigations have proved that bioactive compounds of Ziziphus jujube including triterpenic acids, flavonoids, cerebrosides, phenolic acids, α tocopherol, β carotene, and polysaccharides have anti-proliferative effects on several cancerous cell lines and animals. The present study aimed to cover all prev...
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Genetic barcoding and phylogenetic analysis of dusky cotton bug (Oxycarenus hyalinipennis) using mitochondrial cytochrome c oxidase I gene.
Authors: Khan AZ, Amad I, Shaheen S, Hussain K, Hafeez F, Farooq M, Noor Ul Ayan H Abstract Cotton dusky bug (Oxycarenus spp.) mostly attack on cash crops such as Gossypium, Cola and Hibiscus which affect the national economy therefore sustainable pest management is needed. Cytochrome c oxidase I (COI) gene is utilized as marker gene for DNA barcoding, genetic and ecological study of insects. In present study insect (cotton dusky bug) samples were collected from cotton fields in Faisalabad. COI gene was amplified from genomic DNA of bug and cloned into pTZ57R/T vector (Fermentas). The clone was sent to Macrogen (So...
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research