MiR-30a suppresses non-small cell lung cancer progression through AKT signaling pathway by targeting IGF1R.
This study investigated the expression and role of miR—30a in human NSCLC. The expression of miR—30a is significantly decreased in clinical NSCLC tissues and cell lines. Overexpression of miR—30a inhibited NSCLC cell proliferation, G1/S and S/G2 transition in vitro, whereas suppression of miR—30a facilitated NSCLC cell proliferation, G1/S and S/G2 transition. Using a luciferase reporter assay, insulin—like growth factor 1 receptor (IGF1R) was determined to be a direct target of miR—30a. Furthermore, silencing IGF1R resulted in the same biologic effects of miR&...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Investigation of cell aggregation on the substrate of a parallel-plate flow chamber.
Authors: Bai F, Dong C, Cao C, Sun R Abstract Adhesion of tumor cells (TCs) to polymorphonuclear neutrophils (PMNs) tethered on a monolayer of vascular endothelial cells (ECs) in shear flows is investigated theoretically. Both TC—PMN and PMN—EC aggregations are modeled using the population balance equations (PBEs). Parameters in the model obtained by curve fitting show that an increase of shear rate or viscosity will suppress the formation of aggregates and promote the breakage of them. Analysis on the collision frequency suggests that the averaged encounter duration is affected by viscosity. Ba...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Evaluation of FGFR2 gene polymorphism in women with breast cancer.
Authors: Salehi Z, Afzali S, Shabanipour S, Rahimi A Abstract Breast cancer (BC) is one of the most frequent cancers among women worldwide. It is a complex polygenic disorder that genetic factors play an important role in disease etiology. The highly significant association of the Fibroblast growth factor receptor 2 (FGFR2) locus with breast cancer risk has been replicated in multiple genome association study; however, it's mechanism of action remains unclear. The aim of this study was to investigate whether the single nucleotide polymorphism (SNP) C—906T within intron 2 of FGFR2 is responsible for susceptibi...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

siRNA-mediated silencing of MDR1 reverses the resistance to oxaliplatin in SW480/OxR colon cancer cells.
Authors: Montazami N, Kheir Andish M, Majidi J, Yousefi M, Yousefi B, Mohamadnejad L, Shanebandi D, Estiar MA, Khaze V, Mansoori B, Baghbani E, Baradaran B Abstract One of the most challenging aspects of colon cancer therapy is rapid acquisition of multidrug resistant phenotype. The multidrug resistance gene 1 (MDR1) product, p—glycoprotein (P—gp), pump out a variety of anticancer agents from the cell, giving rise to a general drug resistance against chemotherapeutic agents. The aim of this study was to investigate the effect of a specific MDR1 small interference RNA (siRNA) on sensitivity of ox...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Success of the PCR-based replication assay depends on the number of methylation sensitive restriction sites in the PCR amplifying region.
Authors: Metta MK, Tantravahi S, Kunaparaju R Abstract The PCR—based replication assay is one of the most simple, quick and economical methods for the analysis of episomal replication. However, in spite of its advantages the method has not been able to replace the southern—based replication assay, the latter of which is a tedious and time—consuming process. This is due to the generation of spurious amplification products in the PCR—based replication assay. The replication assay is based on the use of methylation—sensitive restriction endonucleases (eg. DpnI, MboI) t...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Letter to Editor: Cytochrome b gene expression in down syndrome subjects.
Authors: Salemi M, Barone C, Romano C, Grazia Salluzzo M, Galati Rando RG, Scavuzzo C, Salluzzo R, Russo R, Giambirtone M, Scillato F, Romano C PMID: 26068911 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Capacity of a newly isolated fungus Pleurotus eryngii from Tunceli, Ovacik for chemical oxygen demand reduction and biodecolorization of Azo-Dye Congo Red.
Authors: Yildirim N, Gonen U Abstract Biodecolorization of Congo red dye in both agar—plate and agitated liquid culture mediums by newly isolated white rot fungus Pleurotus eryngii has been studied. This fungus isolated from Tunceli—Ovacik province of Turkey. We have also examined the chemical oxygen demand reduction after decolorization under agitated liquid culture medium. For agar plate screening the decolorization capacity of P. eryngii, growth and decolorization halos were determined on saboroud dextrose agar (SDA) plates containing 0.05, 0.1, 0.5, 1 and 2 g/l of Congo red. P. eryngii showe...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

miR-145 regulates chemoresistance in hepatocellular carcinoma via epithelial mesenchymal transition.
Authors: Ju BL, Chen YB, Zhang WY, Yu CH, Zhu DQ, Jin J Abstract Resistance to chemotherapeutic drugs is a major obstacle in hepatocellular carcinoma (HCC) therapy. MicroRNA—145 (miR—145) has been shown to be down—regulated in several cancers and may be involved in the process of carcinogenesis. The present study aimed to evaluate the effects of miR—145 in adriamycin (ADM)—resistant human HCC cells. We found that miR—145 was significantly reduced in HepG2/ADM and HuH7/ADM cells compared with the chemosensitive parental cells. Up—regulation of miR...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

MRJF4, a novel histone deacetylase inhibitor, induces p21 mediated autophagy in PC3 prostate cancer cells.
Authors: Di Giacomo V, Di Valerio V, Rapino M, Bosco D, Cacciatore I, Ciulla M, Marrazzo A, Fiorito J, Di Stefano A, Cataldi A Abstract Autophagy is a cellular defense mechanism which occurs through degradation and recycling of cytoplasmic constituents and represents a caspase—independent alternative to cell death by apoptosis. It is generally accepted that the suppression of autophagy in many cancer cells is directly correlated to malignancy; hence, the control of autophagy genes could represent a target for cancer therapy. The inhibition of cell proliferation through autophagy activation could be an imp...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

microRNA-33A expression is reduced in cerebral cortex in a rat model of ischemic tolerance.
This study examined the microRNA—33a (miR—33a) expression levels in cerebral cortex in rat model of cerebral ischemic tolerance (IT), with 3—nitropropionic acid (3—NPA) preconditioning. Rat model of cerebral IT was established as follows: 14 male Sprague—Dawley rats were randomly divided into two groups, the 3—NPA treated group and the control group. Intraperitoneal injection of 3—NPA or normal saline was performed in 3—NPA treated group and control group, respectively. Middle cerebral artery occlusion (MCAO) was performed in all the rats 3 day...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The effect of moderately halophilic bacteria supernatant on proliferation and apoptosis of cancer cells and mesenchymal stem cells.
Authors: Sarvari S, Seyedjafari E, Amoozgar MA, Bakhshandeh B Abstract Many drug discoveries and developing of their applications has originated from microbial metabolites. The most efforts in development of new drugs are concerned with anti—cancer agents that cause better treatment results, less side effects, and more economical production. Several anti—tumor drugs have been recently extracted from natural microbial products. Among these various microbial diversity, Marin bacteria and Archaea have been considered as important and efficient organisms to serve as manufacturers of diverse bioactive compou...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Vitamin D Deficiency, Obesity and Diabetes.
Authors: Li YX, Zhou L Abstract Obesity and type 2 diabetes mellitus (T2DM) are main chronic diseases harming human health. Although the association between obesity and T2DM is well established, the molecular mechanism is still unclear. Accumulating evidence suggests vitamin D plays a role in the development of these diseases. Vitamin D is a necessary nutrient for humans. People usually do not pay attention to supplementing vitamin D, since vitamin D can be produced when their skin is exposed to the sunlight. Nevertheless, even in highly sunny regions, vitamin D deficiency exists, suggesting vitamin D deficiency is...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Regional and cellular distribution of monocarboxylate transporters 13 and 14 in the cattle gastrointestinal tract.
This study is the first to describe the precise cellular localization of the orphan monocarboxylate transporters (MCT13) and (MCT14) in the physiologically distinct regions of the gastrointestinal tract of mammals. The present study demonstrated conclusively the regional distribution and relative expression levels of MCT13 and MCT14 on both mRNA and protein levels in the cattle gastrointestinal tract. The mRNA expression levels of MCT13 and MCT14 in the rumen, abomasum, jejunum, cecum, and proximal colon of cattle were examined using quantitative real time—PCR analysis. The precise cellular location of MCT13 and ...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

TLR3 and its roles in the pathogenesis of type 2 diabetes.
Authors: Sepehri Z, Kiani Z, Javadian F, Akbar Nasiri A, Kohan F, Sepehrikia S, Javan Siamardi S, Aali H, Daneshvar H, Kennedy D Abstract Type 2 diabetes (T2D) is the most prevalent non—infectious disease and leads to several complications including nephropathy and retinopathy. The mechanisms and signaling molecules responsible for the development and progression of T2D, as well as its associated complications are yet to be identified. It would appear that genetic backgrounds and immunological parameters of people susceptible to T2D may play important roles in induction of T2D. TLRs participate in several...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Establishment of plasma microRNA detection method by using taqman probe based quantitative reverse transcription PCR.
In this study, we tried to establish a reliable method to quantify the plasma miRNAs. We used quantitative real—time PCR with taqman probes to detect the plasma miR—153 level. Three controls were used in this study, including two external miRNAs control from C. elegans miRNAs (cel—miR—54 and cel—miR—238) and one internal control (hsa—miR—486). All of these controls were stable in plasma and the cel—miR—238/cel—miR—54/hsa—miR—486 combination could improve the normalization process. The ...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effect of Lead stress on phosphatase activity and reducing power assay of Triticum aestivum.
Authors: Gubrelay U, Agnihotri RK, Shrotriya S, Sharma R Abstract Lead (Pb) is a highly toxic heavy metal for both plants and animals; the environment is increasingly polluted with heavy metals and reduces crop productivity. Plants possess homeostatic mechanisms that allow them to keep correct concentrations of essential metal ions in cellular compartments and to minimize the damaging effects of an excess of nonessential ones. One of their adverse effects on plants are the generation of harmful active oxygen species, leading to oxidative stress and the antioxidative activity seems to be of fundamental importance fo...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

AIM2 inflammasome is dispensable for the host defense against Pseudomonas aeruginosa infection.
In this study, we found that P. aeruginosa infection induced degradation of AIM2 protein in macrophages and induction of AIM2 mRNA expression in macrophages and in the lung of mice. Interestingly, P. aeruginosa infection induced a similar level of IL—1β, IL—6 and TNF production in wild—type and AIM2—deficient mice. Similarly, no significant differences in bacterial clearance, neutrophil infiltration and NF—κB activation were observed between wild—type and AIM2—deficient mice following P. aeruginosa lung infection. Our data suggest that AIM2 inf...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Cloning, purification and characterization of a thermostable β-galactosidase from Bacillus licheniformis strain KG9.
Cloning, purification and characterization of a thermostable β-galactosidase from Bacillus licheniformis strain KG9. Cell Mol Biol (Noisy-le-grand). 2015;61(3):71-8 Authors: Matpan Bekler F, Stougaard P, Güven K, Gül Güven R, Acer Ö Abstract A thermo— and alkalitolerant Bacillus licheniformis KG9 isolated from Taşlıdere hot water spring in Batman/Turkey was found to produce a thermostable β—galactosidase. Phylogenetic analysis showed that the 16S rRNA gene from B. licheniformis strain KG9 was 99.9% identical to that of the genome sequenced B. ...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

HOTAIR inhibited intracellular Ca2+ via regulation of Cav1.2 channel in human cardiomyocytes.
Authors: Tan WL, Xu M, Liu Z, Wu TY, Yang Y, Luo J, Yang J, Luo Y Abstract HOTAIR, a long non—coding RNA (lncRNA), is reported to regulate chromatin organization and promote tumor progression. However, little is known about the roles of this gene in the modulation of calcium homeostasis in human cardiomyocytes. In the present study, we demonstrated that up—regulation of HOTAIR could suppress the expression of CaV1.2 in human cardiomyocytes. However, HOTAIR knockdown promoted CaV1.2 expression in human cardiomyocytes. In addition, we found that HOTAIR overexpression significantly reduced the intr...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

MicroRNA profiling during germline differentiation of mouse embryonic stem cells.
Authors: Ebrahimzadeh-Vesal R, Shokrgozar MA, Nayernia K, Teimoori-Toolabi L, Estiar MA, Miryounesi M, Nourashrafeddin S, Modarressi MH Abstract MicroRNAs are new classes of small non—coding regulatory RNAs which control degradation or suppress translation of its target mRNAs by sequence complementarity. Mature microRNAs are enriched in embryonic stem cells and play important roles in controlling stem cell self—renewal as well as control of differentiation. There is significant evidence that microRNAs are involved in the regulation of stem cell differentiation. The male mouse Embryonic Stem Cell...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Human Toll like receptor 4 gene expression of PBMCs in diabetes mellitus type 2 patients.
In this study, we evaluate the expression of the TLR4 in patients with diabetes mellitus type 2 (DM2) in comparison to healthy controls. Expression of TLR4 in 32 human peripheral blood mononuclear cells (PBMCs) of patients with DM2 and 20 control samples was assessed using Real—Time PCR technique. For each patient, body mass index (BMI) and blood glucose levels were measured. The results of Real—Time PCR showed a 5—folds increase in expression of TLR4 on the PBMCs of DM2 patients in comparison to controls. No correlation was observed between the TLR4 expression and sex or BMI. Our results conf...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Angiotensin II Promotes Atherogenesis through upregulating the Expression of Connexin 43 in Dendritic Cells.
Authors: Nie W, Yan H, Li S, Zhu W, Fan F, Zhu J Abstract It is known, for a long time, that angiotensin II (Ang II) could contribute to atherogenesis (AS) and plaque vulnerability, however the underlying mechanisms are poorly understood. Dendritic cells (DCs) are critical for the development of both inflammation and atherogenesis. In the present study, we tried to investigate the influence of AngII on the expression of connexin43 (Cx43) in DCs, as well as the effect of AngII on AS. After mouse bone marrow—derived dendritic cells (BMDCs) were treated by Ang II with or without Valsartan, the expression of ...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Withaferin A attenuates lipopolysaccharide-induced acute lung injury in neonatal rats.
Authors: Gao S, Li H, Zhou XQ, You JB, Tu DN, Xia G, Jiang JX, Xin C Abstract Withaferin A (WFA) is an active compound from Withania somnifera and has been reported to exhibit a variety of pharmacological activities such as anti—inflammatory, immunomodulatory and anti—tumor properties. In the present study, we investigated the potential protective role of WFA on acute lung injury in neonatal rats induced by lipopolysaccharide (LPS). We found that WFA significantly attenuated the pathological changes of lungs induced by LPS injection. Administration with WFA obviously decreased pulmonary neutroph...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Different components of chicken ovalbumin extract promote different cell proliferation.
Authors: Ruan GP, Yao X, Liu JF, Wang JX, Pan XH Abstract The chicken ovalbumin extracts could promote cell survival and proliferation. In the present study, the different components in chicken ovalbumin extracts were further separated to find the component primarily responsible for promoting cell proliferation. Components of differing molecular weight were separated from chicken ovalbumin extracts by ultrafiltration. Different components were co—cultured with different cells at different final concentrations, and the effects on cell proliferation were subsequently determined by a CellTiter 96 Aqueous One...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

In Vitro and In Vivo investigations on fibronectin coated and hydroxyapatite incorporated scaffolds.
In conclusion, fabrication of nanofiberous scaffold with this combination of biochemical composition and surface stimulation caused improved biodegradability and biocompatibility of the scaffold which are desirable in more effective tissue regeneration. PMID: 26255261 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Protective effect of bioactive peptide carnosine against lead-induced oxidative stress in kidney of rats.
Authors: Hasanein P, Teimuri-Far M Abstract Oxidative stress is among the mechanisms involved in renal injury. We aimed to investigate the protective effects of bioactive peptide carnosine on lead induced oxidative stress and nephrotoxicity in rats. Animals received an aqueous solution of lead acetate (500 mg Pb/L in the drinking water) and/or carnosine (10 mg/kg, i.g.) for eight weeks. Then rats were weighed and used for biochemical, histological and oxidant/antioxidant evaluations. Lead—induced oxidative stress in renal tissue was indicated by a significant increase in the renal contents of malondialdeh...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Early intracellular signaling events induced by in vitro metreleptin administration in cardiac myocytes and uterine smooth muscle cells.
Authors: Choi SK, Park S, Choi Y, Moon HS Abstract Intracellular signaling pathways regulated by leptin have largely been studied in metabolically important organs such as adipose tissue and peripheral blood mononuclear cells, suggesting that leptin plays a key role in pathophysiology of insulin resistance. However, whether synthetic analog of leptin, metreleptin, has similar effects on cardiac myocytes (CM) and uterine smooth muscle cells (USMC) has not yet been studied. Hence, in order to address these questions, we extended previous observations and investigated in vitro signaling study whether metreleptin may a...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The association between 1349T>G polymorphism of ApE1 gene and the risk of prostate cancer in northern Iran.
The association between 1349T>G polymorphism of ApE1 gene and the risk of prostate cancer in northern Iran. Cell Mol Biol (Noisy-le-grand). 2015;61(4):21-4 Authors: Pournourali M, Tarang AR, Yousefi M Abstract Prostate cancer (PCa) is the most common malignancy in men and the fourth most common cause of death based on cancer all over the world. Many genes has been shown to be involved in the progress of the prostate cancer. Human apurinic/apyrimidinic endonuclease 1 (APE1) is a multifunctional protein that has an important role in the base excision repair (BER) pathway. The aim of this study wa...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Identification and expression analysis of 4-Coumarate: Coenzyme A ligase gene family in Dryopteris Fragrans.
In conclusion, we identified 4CLs genes in ferns D. fragrans and analyzed the expressions of these genes, and finally explored the relationship between the expressions of 4CLs and syntheses of flavonoids and lignin. PMID: 26255265 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Long non-coding RNA HOTTIP promotes tumor growth and inhibits cell apoptosis in lung cancer.
In this study, we investigated the role of long non—coding RNA HOTTIP in the tumor growth of lung cancer. Initially, we found that expression of HOTTIP was significantly elevated in 20 cases of lung cancer. HOTTIP was also differentially expressed in a consecutive of lung cancer cell lines. Furthermore, specific shRNA against HOTTIP was employed to deplete expression of HOTTIP in A549 cells and NCI—H446 cells. After successfully depletion of HOTTIP, cell proliferation and colony formation were significantly inhibited in vitro. Tumor growth in vivo was also suppressed after depletion of HOTTIP in a mouse...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Immunohistochemical study of RhoC GTPase in oral squamous cell carcinoma.
This study aimed to evaluate the expression pattern of RhoC in OSCC protein by immunohistochemistry in situ. Immunohistochemical reactions were performed for RhoC by the method of avitina—biotin—peroxidase activity in samples OSCC: well differentiated (BD, n=6), moderately differentiated (MD, n=24) and poorly differentiated (PD, n=13). The morphometry was taken by QuickScore (percentage and intensity of staining) and only intensity staining. There was no statistical difference (p>0.05) through none of the modes of morphometric analysis between BD, MD and PD. And the RhoC staining was not associated w...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Radioprotective effect of chicory seeds against genotoxicity induced by ionizing radiation in human normal lymphocytes.
Authors: Hosseinimehr SJ, Ghaffari-Rad V, Rostamnezhad M, Ghasemi A, Allahverdi Pourfallah T, Shahani S Abstract The search for less—toxic radioprotective agents has led to a growing trend towards natural products. Protective effect of the methanolic extract of chicory seeds (MCS) was investigated against genotoxicity induced by ionizing radiation in human lymphocytes. Human peripheral blood samples were collected and incubated with MCS at different concentrations (10, 50, 100, and 200 μg/mL) for two hours. The whole blood samples were exposed in vitro to X—ray at dose 2.5 Gy. Then, th...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Mutation analysis of the CYP21A2 gene in congenital adrenal hyperplasia.
Mutation analysis of the CYP21A2 gene in congenital adrenal hyperplasia. Cell Mol Biol (Noisy-le-grand). 2015;61(4):51-5 Authors: Forouzanfar K, Seifi M, Hashemi-Gorji F, Karimi V, Estiar MA, Karimoei M, Sakhinia E, Karimipour M Abstract Congenital adrenal hyperplasia (CAH) is an inherited autosomal recessive enzymatic disorder involving the synthesis of adrenal corticosteroids. 21—Hydroxylase deficiency (21—OHD) is the most common form of the disease which is observed in more than 90% of patients with CAH. Early identification of mutations in the genes involved in this di...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

miR-106a* inhibits the proliferation of esophageal carcinoma cells by targeting CDK2-associated Cullin 1 (CACUL1).
In this study, we explored the expression and anti—oncogenic roles of miR—106a* in human EC. The expression of miR—106a* is significantly decreased in EC tissues and EC cell lines. Overexpression of miR—106a* suppressed EC cell proliferation, clonogenicity, G1/S transition, and induced apoptosis in vitro, but inhibition of miR—106a* facilitated cell proliferation, clonogenicity, G1/S transition. Luciferase reporter assay results showed that CDK2—associated Cullin 1 (CACUL1) was a direct target of miR—106a* in EC cells. Moreover, silencing CACUL1 resulted...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Genetics and evolution of plumage color in Crested Ibis: Analysis of the melanocortin-1 receptor (MC1R).
Authors: Yang J, Liu X, Zhang J, Qing B, Lu B Abstract The melanocortin—1—recepter gene (MC1R), an important regulator in melanin synthesis, may cause different plumage color patterns in birds: gain—of— function mutations lead to the synthesis of eumelanin, whereas loss—of—function mutations help to generate pheomelanin synthesis. We had chosen MC1R as a candidate gene for the depigmentation of crested ibis, cloned and sequenced the crested ibis MC1R gene the first time. The crested ibis MC1R sequence, highly conserved with other birds during evolution, ha...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The association of ApE1 -656T>G and 1349T>G polymorphisms with breast cancer susceptibility in northern Iran.
The association of ApE1 -656T>G and 1349T>G polymorphisms with breast cancer susceptibility in northern Iran. Cell Mol Biol (Noisy-le-grand). 2015;61(4):70-4 Authors: Mashayekhi F, Yousefi M, Salehi Z, Saedi HS, Pournourali M Abstract Breast cancer is the most common cancer and one of the main causes of cancer—related death in the world and has become a major public health concern. Many genes have been shown to be involved in the progress of the breast cancer. Human apurinic/apyrimidinic endonuclease 1 (APE1) is a multifunctional protein that has an important role in the base excision r...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Glutamate promotes neural stem cell proliferation by increasing the expression of vascular endothelial growth factor of astrocytes in vitro.
In this study, we investigated the effect of glutamate on the proliferation of rat embryonic neural stem/progenitor cells (NSCs) through regulating the vascular endothelial growth factor (VEGF) expression of astrocytes (ASTs) in vitro, and the cyclin D1 expression of NSCs. The results showed that glutamate promoted the expression and secretion of VEGF of rat astrocytes by activating group I mGluRs. Astrocyte conditioned medium—containing Glu [ACM (30%)] promoted the proliferation of embryonic NSCs compared with normal astrocyte conditioned medium+Glu [N—ACM (30%)+Glu (30 μM)] by increasing cell act...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Molecular cloning and activity analysis of a seed-specific FAD2-1B gene promoter from Glycine max.
In this study, the FAD2—1B gene was determined to be highly expressed in soybean seeds using quantitative real—time PCR(qRT—PCR). To investigate the expression pattern and activity of the FAD2—1B promoter, a 1929 bp 5'—upstream genomic DNA fragment, named PF, was isolated according to the soybean genomic sequence. Sequence analysis revealed the presence of many motifs related to seed—specific promoters in the PF fragment, such as E—box, SEF4, Skn—1 motif, AACACA, AATAAA and so on. Tobacco transgenics carrying the gus reporter gene driv...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Surveillance of gram-positive cocci infections and drug resistance.
In this study, the prevalence of gram—positive cocci isolates and the characteristics of multiple drug resistances in patients were investigated. Antibiotic resistances were determined in the clinical microbiology laboratory with the methodology of the CLSI (2012). The software WHONET5.4 and SPSS13.0 were used for statistical analysis. There were a total of 6211 gram—positive cocci isolates, comprised of 2255 (36.3%) coagulase (—) staphylococci, 1277 (20.6%) staphylococci aureus, 1109 (17.9%) enterococcus faecalis, and 1045 (16.8%) enterococcus faecium. The proportion of Methicillin resistant ...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Expression levels of heat shock protein 60 and glucose-regulated protein 78 in response to trimethylamine-N-oxide treatment in murine macrophage J774A.1 cell line.
This study was therefore designed to evaluate the possible effect(s) of TMAO on the expression of HSP60 and GRP78 at the mRNA and protein levels. Murine macrophage J774A.1 cells were treated with micromolar concentrations of TMAO and 4—phenylbutyric acid (4—PBA), a chemical chaperon, for different time intervals. Tunicamycin was also used as a control for induction of endoplasmic reticulum stress. Tunicamycin greatly increased both mRNA and protein levels of GRP78. Similarly but to a lesser extent compared to tunicamycin, TMAO also increased mRNA and protein levels of GRP78 in a dose and time—...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Comparative phenotypic and genotypic discrimination of methicillin resistant and susceptible Staphylococcus aureus in Egypt.
This study presented the first baseline information on the characterization of MRSA and MSSA in Egypt. PMID: 26429300 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Production and purification of novel thermostable alkaline protease from Anoxybacillus sp. KP1.
In this study, an extracellular novel alkaline protease (EC 3.4.21—24, 99) from a thermophilic and aerobic strain of Anoxybacillus sp. KP1 has been studied. Maximum protease activity was obtained at 50 degC at pH 9.0 after 24 hours of incubation. Among the carbon and nitrogen sources used; the optimum protease production was with soluble starch, maltose, urea and casamino acid. The enzyme was purified by ammonium sulphate precipitation and Sephadex G—75 gel chromatography. Molecular weight of purified enzyme was determined as 106 kDa by SDS—PAGE. Purified protease was stable at 50-60 degC and ...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Targeting microbiota-mitochondria inter-talk: Microbiota control mitochondria metabolism.
Authors: Saint-Georges-Chaumet Y, Attaf D, Pelletier E, Edeas M Abstract Our aim is to highlight the subtle relationship that exists between microbiota and mitochondria. Microbiota targets mitochondria by modulating the Reactive Oxygen Species (ROS) production and the mitochondrial activity through interactions with toxins, proteins or other metabolites released by gut microbiota. The intriguing relationship that exists between mitochondria and microbiota is strengthened by the probable prokaryotic origin of mitochondria. Emerging data implicates a role for ROS, nitric oxide, Short Chain Fatty Acids and hydrogen su...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Micro-RNAs: The new potential biomarkers in cancer diagnosis, prognosis and cancer therapy.
Authors: Mansoori B, Mohammadi A, Shirjang S, Baradaran B Abstract MicroRNAs (miRNAs) are a large class of small noncoding RNAs approximately 22 nucleotides in length. They are the main regulators of gene expression, regulating specific oncogenes, tumor suppressors, cancer stem cells and metastasis. MicroRNAs have become valuable to cancer research in recent years. They appear as a significant biomarker in tumorigenesis. Briefly, the capacities of miRNA to identify between tumor and normal tissue, to distinguish between various subgroups of tumors and to foretell results or responses to therapy have attracted scien...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

SERS and DFT study of p-hydroxybenzoic acid adsorbed on colloidal silver particles.
In this study, normal Raman spectra of p—hydroxybenzoic acid (PHBA) powder and its surface—enhanced Raman scattering (SERS) spectra in silver colloidal solutions were measured under near infrared excitation conditions. In theoretical calculation, two models of PHBA adsorbed on the surfaces of silver nanoparticles were established. The Raman frequencies of these two models using density functional theory (DFT) method were calculated, and compared with the experimental results. It was found that the calculated Raman frequencies were in good agreement with experimental values, which indicates that there ar...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Association of polymorphisms in PRKCI gene and risk of prostate cancer in a sample of Iranian Population.
In conclusion, the results revealed that PRKCI rs546950 variant decreased the risk of PCa in an Iranian population. Further studies with larger sample sizes and different ethnicities are required to confirm our findings. PMID: 26475383 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Citrate and celecoxib induce apoptosis and decrease necrosis in synergistic manner in canine mammary tumor cells.
In conclusion, the present study indicates significant cytotoxic and apoptotic effects of citrate and celecoxib coadministration on CF41—Mg cells, and proposes new strategies for counteracting cancer cells proliferation and overcoming chemo resistance. PMID: 26475384 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Coexistence of plasmid-mediated quinolone resistance determinants and AmpC-Beta-Lactamases in Escherichia coli strains in Egypt.
Authors: Abd El-Aziz NK, Gharib AA Abstract Three kinds of plasmid—mediated quinolone resistance (PMQR) determinants (qnr genes, qepA and aac(6')—Ib—cr) have been discovered and shown to be widely distributed among clinical isolates. To characterize the prevalence of PMQR determinants among AmpC—producing E. coli strains in food—producing animals and animal by—products in Egypt, twenty—nine E. coli strains were tested for their susceptibilities to antimicrobials and screened for PMQR determinants and AmpC Beta lactamases using PCR and pl...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Purification and dose-dependent toxicity study of abrin in swiss albino male mice.
In conclusion, abrin intoxication caused toxic effects and severe damages on studied organs mediated through alteration in biochemical and hematological variables, lipid peroxidation, and degeneration. PMID: 26475386 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Activity of dental pulp cells in semisolid 3D cultures initiated by transforming growth factor-β1 and bone morphogenetic protein 2, 4.
Activity of dental pulp cells in semisolid 3D cultures initiated by transforming growth factor-β1 and bone morphogenetic protein 2, 4. Cell Mol Biol (Noisy-le-grand). 2015;61(5):45-8 Authors: Suzergoz F, Sepet E, Erdem AP, Cinar S, Ikikarakayali G, Erdem MA, Gurol AO Abstract The intention of this study was to investigate the effect of modified 3D culture conditions on dental pulp cells (DPCs). DPCs were isolated from extracted primary molar, premolar, and wisdom teeth. Tooth samples were divided into three groups as control group; plated into methyl cellulose medium without any supplementation, g...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research