The correlation between Pax5 deletion and patients survival in Iranian children with precursor B-cell acute lymphocytic leukemia.
Authors: Moafi A, Zojaji A, Salehi R, Najafi Dorcheh S, Rahgozar S Abstract Despite advances in treatment, children with acute lymphoblastic leukemia (ALL) still experience drug resistance and relapse. Several gene mutations are involved in the onset of this disease and resistance to therapy. The present study examines the incidence of IKZF1, CDKN2A/B, PAX5, EBF1, ETV6, BTG1, RB1, JAK2, and Xp22.33 gene deletions/duplications associated with pediatric ALL in Iran and investigates the possible effect of these mutations on drug resistance. Three-year disease-free survival (3DFS) was evaluated for children diagnosed w...
Source: Cellular and Molecular Biology - September 9, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Risk factors associated with oral and maxillofacial benign tumors: A case-control study.
This study aimed to investigate the risk factors for oral and maxillofacial benign tumors (OMFBTs). A total of 138 patients diagnosed with OMFBTs between September 2010 and September 2015 were retrospectively analyzed. Clinical data including demographic characteristics, smoking and drinking status, dietary habit, oral hygiene and tumor related family history were collected and compared with 134 cases of healthy people who visited the hospital for physical examination during the same time. Logistic regression analysis was performed for multivariable regression analysis. OMFBTs was associated with smoking more than 20 cigar...
Source: Cellular and Molecular Biology - September 9, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effect  of sucrose concentrations on Stevia rebaudiana Bertoni tissue culture and gene expression.
Effect of sucrose concentrations on Stevia rebaudiana Bertoni tissue culture and gene expression. Cell Mol Biol (Noisy-le-grand). 2017 Aug 30;63(8):33-37 Authors: Ghorbani T, Kahrizi D, Saeidi M, Arji I Abstract Stevia rebaudiana (Bert.) Bertoni is known as sweet plant which it contains a high level of steviol glycosides in the leaves.  This plant has been used from centuries ago as a sweetener for tea. One of the most important steviol glycosides is stevioside that is attractive for diabetic persons. Tissue culture is the only rapid process for the mass propagation of stevia. One of the...
Source: Cellular and Molecular Biology - September 9, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Differences in expression of serum protein in patients with psoriasis vulgaris and blood heat syndrome and healthy volunteers.
Authors: Hao P, Yang C, Yan X, Li T, Zhang J, Yu Q, Zhang X, Zhang Y Abstract To explore the mechanism of psoriasis vulgaris (PV), serum protein expression profiles between PV patients with blood-heat syndrome and healthy volunteers were detected by isobaric tags for relative and absolute quantitation (iTRAQ). First, sera from 15 PV patients with blood-heat syndrome and 10 healthy volunteers were collected; then, serum proteins were separated and hydrolyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a specific iTRAQ marker enzyme respectively after further purification and protein a...
Source: Cellular and Molecular Biology - September 9, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Susceptibility of herpes simplex virus type 1 to monoterpenes thymol, carvacrol, p-cymene and essential oils of Sinapis arvensis L., Lallemantia royleana Benth. and Pulicaria vulgaris Gaertn.
CONCLUSION: These results indicate that compounds affected HSV-1 mostly before adsorption and might interact with the viral envelope. Thymol exhibited a high selectivity index and seems to be a promising candidate for topical therapeutic application as antiviral agent for treatment of herpetic infections. PMID: 28886313 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - September 9, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Alterations on high HbF levels may be associated with KLF1 gene mutations.
In this study, in order to determine the relationship between the KLF1 mutations and the high HbF levels three exons along with the 5'-UTR and 3'-UTR regions of the KLF1 gene were sequenced of 53 volunteers. In this study, 3 variations in the non-coding regions of the KLF1 gene were not associated with a high level of HbF. Five variations were detected in the second exon of KLF1 gene. One of these is a frame shift that occurs when GG bases are inserted between the 59-60 codons, and the other four variations occur as a base substitution variations.  No correlation was found between high HbF levels and neutral variants....
Source: Cellular and Molecular Biology - September 9, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The protective effect of cinnamaldehyde on  lipopolysaccharide induced acute lung injury in mice.
The protective effect of cinnamaldehyde on lipopolysaccharide induced acute lung injury in mice. Cell Mol Biol (Noisy-le-grand). 2017 Aug 30;63(8):58-63 Authors: Huang H, Wang Y Abstract The study wasintended to examinethe protective effect of cinnamaldehyde (CM) on lipopolysaccharide (LPS)-induced acute lung injury (ALI) mice model.The results of the investigation confirmed that, LPS induced inflammatory cytokines such as TNF-α, IL-6, IL-13 and IL-1β were significantly decreased by CM. CM also up-regulated level of IL-10. We found that CM significantly attenuated LPS-induced TLR4 expre...
Source: Cellular and Molecular Biology - September 9, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Is GDF5 gene promoter polymorphism +104T/C associated with osteoarthritis in the Eastern of Turkey population?
Authors: Tülüce Y, Yildirim IH, Özkol H, Edi Z L, Delen V Abstract Osteoarthritis (OA) is the most common form of arthritis. Genetic factors have been shown to play important roles in the etiology of OA. The gene growth differentiation factor 5 (GDF5) has been implicated in skeletal development and joint morphogenesis in human and mice. A functional single nucleotide polymorphism (SNP) +104T/C in the 5'-UTR of GDF5 (rs143383) was reported to be associated with osteoarthritis susceptibility in Han Chinese and Japanese populations. Our objective was to assess whether this SNP was also associated with O...
Source: Cellular and Molecular Biology - September 9, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Potential of Laurencia obtusa as a substrate for the development of a probiotic Saccharomyces cerevisiae.
In conclusion, it was determined that the L. obtusa extract added to the development medium of S. cerevisiae caused differences in the synthesis of some vitamins and fatty acids. PMID: 28886317 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - September 9, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

New insights into the expression profile of MicroRNA-34c and P53 in infertile men spermatozoa and testicular tissue.
In this study, we investigate the role of miRNAs in infertile males.The patients were assigned to five groups based on semen analysis (n=55), including normozoospermic (N), moderate oligoasthenoteratozoospermic (MOAT), severe oligoasthenoteratozoospermic (SOAT), obstructive azoospermia (OA) and non-obstructive azoospermia (NOA). Quantitative RT-PCR was recruited to study the expression of miR-34c and tumor suppressor p53 gene. In addition, malondialdehyde (MDA) and DNA fragmentation was measured. Network analysis was performed using Pathway Studio web tool (Elsevier). Our results revealed statistically significan...
Source: Cellular and Molecular Biology - September 9, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Morphokinetic analysis of human embryo development and its relationship to the female age: a retrospective time-lapse imaging study.
Authors: Akhter N, Shahab M Abstract Time-lapse imaging technique has provided embryologists with a unique chance of studying the embryo morphokineticsto select the most viable embryos for implantation in the field of IVF (In vitro fertilization).The conventional method of morphological evaluations has proved that maternal age affects the human embryo quality. This retrospective study carried out at Islamabad Clinic Serving Infertile Couples, Islamabad, Pakistan mainly focuses on the effect of female age on human embryo morphokinetics. A total number of 200 patients undergoing ICSI treatment cycles at the clinic we...
Source: Cellular and Molecular Biology - September 9, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Investigation of the irisin, preptin  and adropin levels in the blood serum of Alburnus tarichi.
This study used 12 adult A. tarichi (6 female and 6 male) obtained from Van Lake (Van, Turkey). The serum irisin, preptin and adropin levels were measured using a commercially available enzyme-linked immunosorbent assay (ELISA) kit to determine correlations between the levels of these three hormones and fish body weight and length.  No statistically significant correlations were detected between the serum irisin, adropin and preptin levels and A. tarichi body weight (p = 0.921, r = -0.031; p = 0.08, r = 0.519; p = 0.461, r = -0.235, respectively) or length (p = 0.901, r = -0.040; p = 0.105, r = 0.490; p = 0.236, r = -...
Source: Cellular and Molecular Biology - September 9, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Relationship between MCP1 (-2518A > G) gene variants and ovarian cancer in Turkish population.
This study suggests that MCP-1 (-2518A>G) AG genotype and G allele could be considered as risk factor for susceptibility to ovarian cancer. PMID: 28886321 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - September 9, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Carnosol inhibits Hedgehog signaling pathway in both LNCaP and DU145 prostate cancer cell lines.
Authors: Gao H, Song Q, Yang J, Yu S, Zhao J, Yu G Abstract To investigate the effect of carnosol on the Hedgehog (HH) signaling pathway in human hormone-dependent prostate cancer cell line LNCaP and hormone-independent prostate cancer cell line DU145. The expression levels of glioma-associated oncogene homolog 1 (Gli1) and Sonic hedgehog (Shh) in human prostate cancer tissues were detected by immunohistochemistry. After treated with carnosol (0.25-16 μmol/L), the cell survival of LNCaP and DU145 cells were detected by MTT assay. The expression levels of Gli1 and Shh mRNA and protein in the two cells were detect...
Source: Cellular and Molecular Biology - September 9, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Genotoxic and cytotoxic potential of Alternanthera Bettzickiana, an important ethno-medicinal plant.
Authors: Akhtar MF, Sharif A, Saleem M, Saleem A, Akhtar B, Raza M, Ijaz H, Shabbir M, Ali S, Sharif A, Nasim MB, Peerzada S Abstract The present study was carried out to investigate the mutagenic and cytotoxic potential of n-hexane and aqueous-methanolic whole plant extracts of Alternanthera bettzickiana. Aqueous-methanolic and n-hexane extracts of Alternanthera bettzickiana extracts were assessed for the mutagenic potential with Salmonella tester strains TA-100 and TA-102 in the presence and absence of the rodent enzyme activation system and cytotoxic potential was assessed by MTT assay. Aqueous-methanolic extrac...
Source: Cellular and Molecular Biology - September 9, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Atypical bacterial etiology of acute respiratory infections and clinical characterizations among Iranian children.
Authors: Halaji M, Hashempour T, Pouladfar GR, Ghasabi F, Khashei R Abstract Acute respiratory infections (ARIs) in children younger than 5 years of age are one of the leading causes of morbidity and mortality, particularly in developing countries. Mycoplasma pneumoniae and Chlamydophila pneumoniae are prevalent causative agents of ARIs, worldwide. We sought M. pneumoniae and C. pneumoniae in respiratory samples from Iranian children with ARIs.  From November 2014 to April 2015, respiratory samples of 150 children aged 1 month to 15 years old were screened for presence of M. pneumoniae and C. pneumoniae. Polym...
Source: Cellular and Molecular Biology - September 9, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Nullification of aspirin induced gastrotoxicity and hepatotoxicity by prior administration of wheat germ oil in Mus musculus: histopathological, ultrastructural and molecular studies.
Authors: Mohamed HRH, Hamad SR Abstract Aspirin (salicylic acetyl acid) is used worldwide to treat various inflammatory conditions and prevent cardiovascular disease, along with reducing the risk of cancer. However, administration of aspirin causes toxic effects, especially in the stomach and liver. Thus, our study examined the protective effect of wheat germ oil on aspirin-induced toxicity in the stomach and liver tissues of albino white mice. Administration of wheat germ oil before aspirin has restored normal hepatic and gastric tissue architecture and DNA integrity has become better than that of a negative healt...
Source: Cellular and Molecular Biology - September 9, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

miR-146a rs2910164 polymorphism might be associated with coronary artery disease risk in Asians.
In conclusion, this meta-analysis found that miR-146a rs2910164 polymorphism was significantly associated with CAD risk. PMID: 28886326 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - September 9, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Association between platelet to lymphocyte ratio (PLR) and overall survival (OS) of hepatocellular carcinoma (HCC): A meta-analysis.
In conclusion, this meta-analysis suggested that PLR was significantly associated with the OS of HCC. PMID: 28886327 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - September 9, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Angiotensin-converting enzyme (ACE) I/D polymorphism is a risk factor of allergic rhinitis.
In conclusion, this meta-analysis indicated that ACE I/D polymorphism was significantly associated with allergic rhinitis risk. PMID: 28886328 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - September 9, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

A case of IgG4-related retroperitoneal fibrosis with multiple involvement.
We report a 79-year-old man with multiple organs involvement of IgG4-RRPF, who developed right lower extremity edema, hemoptysis and fever. The abdomen computed tomography (CT) scan image showed lymph nodes enlargement. The positron emission tomography/CT scan image showed pancreatic malignancy with multiple nodal lymph node metastasis, lung fibroblast proliferation, and right lung apex bullae. The chest CT scan image showed pulmonary multiple lymph nodes with calcification in the mediastinum. Posterior peritoneum magnetic resonance imaging showed the body and tail of the pancreas parenchymatous mass. The serum IgG4 concen...
Source: Cellular and Molecular Biology - September 9, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Cornelia de lange syndrome with thyroid agenesis of an indonesian patient.
We present an 11 months old female Indonesian patient with classic CdLs with congenital hypothyroid. Genetics studies were performed in intron 1, exon 2, exon 10 and exon 22 of NIPBL gene. Thyroid studies (T3, T4, TSH and thyroid scan) were performed. Low level of T3 and T4, and high level of TSH were observed. Thyroid agenesis was found in thyroid scan examination. We detected thyroid agenesis which has been never reported in CdLs patients. We could not find any mutation in intron 1, exon 2, exon 10 and exon 22 of NIPBL gene. Further genetics examinations were necessary whether there is mutation in other locus. PMID: ...
Source: Cellular and Molecular Biology - September 9, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Oncolytic E1B 55KDa-deleted adenovirus replication is independent of p53 levels in cancer cells.
In this study, we investigated the relationship between the efficiency of Ad5dlE1B 55kDa replication and p53 levels in cancer cells. Five transient p53 expression vectors were engineered to expresses different p53 levels in transfected cells. Then, the effect of the variable p53 levels and cellular backgrounds on the replication efficiency of oncolytic Ad5dlE1B 55kDa was evaluated in H1299 and HeLa cell lines. We found that the replication efficiency of these oncolytic viruses is dependent on the status, but not the expression levels, of p53. Ad5dlE1B 55kDa was shown to have selective replication activity in H1299 cells (p...
Source: Cellular and Molecular Biology - August 26, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Hypoxia stimulates neural stem cell proliferation by increasing HIF ‑1α expression and activating Wnt/β-catenin signaling.
Hypoxia stimulates neural stem cell proliferation by increasing HIF‑1α expression and activating Wnt/β-catenin signaling. Cell Mol Biol (Noisy-le-grand). 2017 Aug 15;63(7):12-19 Authors: Qi C, Zhang J, Chen X, Wan J, Wang J, Zhang P, Liu Y Abstract Evidence indicates that after brain injury, neurogenesis is enhanced in regions such as hippocampus, striatum, and cortex. To study the role of hypoxia-inducible factor-1 (HIF‑1α) and Wnt signaling in cerebral ischemia/hypoxia-induced proliferation of neural stem cells (NSCs), we investigated the proliferation of NSCs, expression of HIF...
Source: Cellular and Molecular Biology - August 26, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effects of curcumin plus Soy oligosaccharides on intestinal flora of rats with ulcerative colitis.
Authors: Huang G, Ye L, Du G, Huang Y, Wu Y, Ge S, Yang Z, Zhu G Abstract To explore the therapeutic effect of curcumin (Cur) and soybean oligosaccharides (SBOS) on ulcerative colitis (UC) through testing the intestinal flora and ulcerative colitis (UC). 80 male SD rats were selected divided into four groups with 20 rats in each group: normal group, sulfasalazine (SASP) group, model group and group of curcumin plus soy oligosaccharide. All animals were treated for 4 weeks. In the fifth week rats were decapitated. Macroscopic damage scores of colonic mucosa were calculated. A 4mL blood sample was taken to detect the...
Source: Cellular and Molecular Biology - August 26, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Comparative determination of ventilatory efficiency from constant load and incremental exercise testing.
Authors: Algul S, Ugur FA, Ayar A, Ozcelik O Abstract The analysis of the relationships between minute ventilation (VE) to CO2 output (VCO2), referred to as ventilatory efficiency, in response to incremental exercise testing, is considered a useful index for assessing the presence and severity of cardiopulmonary and metabolic diseases. The effects of constant load exercise testing performed at work intensity associated with anaerobic threshold (AT) and respiratory compensation points (RCP), on the accurate measurements of ventilatory efficiency are not well known. The aim of this present study was to investigate th...
Source: Cellular and Molecular Biology - August 26, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effect of Cdc42 on myocardial ischemia-reperfusion of rats.
Authors: Xu X, Kong L, Song X, Hao Z, Yuan F Abstract To investigate the effects and their possible mechanisms of cell division cycle 42 (Cdc42) to neonatal rat myocardial cells subjected to the ischemia-repefusion. Neonatal rat cardiomyocytes were cultured and then subjected to the ischemia-reperfusion. Experimental groups 1. Control group; 2. Ischemia-repefusion group (I/R group); 3. Oligofectamine group (Oli group); 4. Oligofectamine and antisense oligodeoxynucleotide (AS-ODN) group (As group); 5. Oligofectamine and missense oligodeoxynucleotide (MS-ODN) group (Ms group); 6. SP600125 and Oligofectamine and AS-OD...
Source: Cellular and Molecular Biology - August 26, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Mass spectrometric identification of diagnostic markers for chronic prostatitis in seminal plasma by analysis of seminal plasma protein clinical samples.
In conclusion, we found age-related changes in seminal plasma protein expression. PAEP expression in seminal plasma should be investigated further to evaluate its potential as a diagnostic marker for chronic prostatitis. PMID: 28838337 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - August 26, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Ghrelin and NUCB2/Nesfatin-1 expression in unilateral testicular torsion-induced rats with and without N-acetylcysteine.
Authors: Sarac M, Bakal U, Tartar T, Kuloglu T, Yardim M, Artas G, Aydin S, Kazez A Abstract Testicular torsion (TT) is a common urological problem in the field of pediatric surgery. The degree and duration of torsion determines the degree of testicular damage; however, its effects on the expression of octanoylated ghrelin and nucleobindin 2 (NUCB2) /nesfatin-1 synthetized from testicular tissue remain unclear. We explored the effects of experimentally induced unilateral TT on serum and contralateral testicular tissue ghrelin and NUCB2/nesfatin-1 levels, and determined whether N-acetyl cysteine (NAS) treatment had ...
Source: Cellular and Molecular Biology - August 26, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Histologic analyses of different concentrations of TritonX-100 and Sodium dodecyl sulfate detergent in lung decellularization.
In this study, we aimed to investigate a comparison of different concentrations of Triton X-100 and Sodium dodecyl sulfate detergents in lung decellularization in order to evaluate the effects of different concentrations and times of mentioned detergents on three dimensional and ECM proteins lung. Two detergents (Triton-X100 and Sodium dodecyl sulfat) were used with different concentrations for decellularizing rat lungs for maintaining of three-dimensional lung architecture and ECM protein compositions which have significant roles in differentiation and migration of stem cells. Results showed that SDS 0.05%, 0.1% and Trito...
Source: Cellular and Molecular Biology - August 26, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Isolation, identification and characterization of Morganella morganii from Naja naja atra in Beijing, China.
We report the isolate of M. morganii from Naja naja atra following infections of heart, lung and liver. Seven strains were confirmed using 16S rDNA amplified and sequences. Antimicrobial susceptibility testing of M. morganii isolates demonstrated ubiquitous resistance to ampicillin, amoxicillin/clavulanic acid, cefazolin, cephalothin, sulfamethoxazole/trimethoprim, sulfamethoxazole et al. However, M. morganii ubiquitous susceptible to piperacillin, ampicillin/sulbactam, piperacillin/tazobactam, cefixime et al. Further investigate display gyr B and Sul2 genes presence in all M. morganii isolates. AAC(3)-II was found in E2, ...
Source: Cellular and Molecular Biology - August 26, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Chicken egg-white extracts promote OCT4 and NANOG expression and telomeres growth in 293T cells.
Authors: Ruan GP, Yao X, Shu J, Liu JF, Pang RQ, Pan XH Abstract It will have broad applications in cell biology if one of egg cell extracts has the roles to promote cell proliferation and reprogramming. It will provide a new method for easier reprogramming somatic cells and promote cell proliferation. We found chicken egg-white extracts have roles to promote cell proliferation and reprogramming. The different ingredients were then assessed for cell proliferation activity and somatic cell reprogramming. Chicken egg-white extract ingredients that were less than 3 kDa (LT3K) promoted cell proliferation. Those ingredi...
Source: Cellular and Molecular Biology - August 26, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The association between PAI-1 -675 4G/5G polymorphism and type 2 diabetes mellitus.
In conclusion, this case-control study suggested that PAI-1 -675 4G/5G polymorphism was not associated with T2DM risk in this population. PMID: 28838342 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - August 26, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

In vitro evaluation of cytotoxic, anti-proliferative, anti-oxidant, apoptotic, and anti-microbial activities of Cladonia pocillum.
Authors: Ersoz M, Coskun ZM, Acikgoz B, Karalti I, Cobanoglu G, Cesal C Abstract The aim of this study was to investigate the anti-proliferative, apoptotic, cytotoxic, and anti-oxidant effects of extracts from the lichen Cladonia pocillumon human breast cancer cells (MCF-7), and to characterize the anti-microbial features.  MCF-7 cells were treated with methanolic C. pocillum extract for 24h. The cytotoxicity of the extract was tested with MTT. Moreover, its anti-proliferative effects were examined with immunocytochemical method. Apoptosis and biochemical parameters were detected in MCF-7. The methanol and chl...
Source: Cellular and Molecular Biology - August 26, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Inflammatory and immune responses to a 3-day period of downhill running in active females.
Authors: Jafariyan S, Monazzami A, Nikosefat Z, Nobahar M, Yari K Abstract Exercise-induced muscle damage (EIMD) is accompanied by inflammatory and immune responses. However, due to the repeated bout effect, there will probably be less EIMD. Hence, the purpose was to investigate inflammatory and immune responses over a three-day period of downhill running in active females. Eleven moderately trained healthy females performed three 60-minute bouts of downhill running in -13.5% grade, separated by 24 hours, at a speed eliciting 70-80% of their VO2peak on level grade. Delayed onset muscle soreness (DOMS), range of mot...
Source: Cellular and Molecular Biology - August 26, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Prediction of G gene epitopes of viral hemorrhagic septicemia virus and eukaryotic expression of major antigen determinant sequence.
In conclusion, the main antigen domain of VHSV G was successfully expressed in the Bac-to-Bac baculovirus system. PMID: 28838345 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - August 26, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Molecular effects of leptin on peroxisome proliferator activated receptor gamma (PPAR- γ) mRNA expression in rat's adipose and liver tissue.
Molecular effects of leptin on peroxisome proliferator activated receptor gamma (PPAR-γ) mRNA expression in rat's adipose and liver tissue. Cell Mol Biol (Noisy-le-grand). 2017 Aug 15;63(7):89-93 Authors: Abbasi A, Moghadam AA, Kahrarian Z, Abbsavaran R, Yari K, Alizadeh E Abstract Leptin is a 16-kDa peptide hormone secreted by adipose tissue that participates in the regulation of energy homeostasis. The aim of this study was to determine the effect of leptin injection on mRNA expression of peroxisome proliferator-activated receptor gamma (PPAR-γ) and comparison of PPAR-γ mRNA express...
Source: Cellular and Molecular Biology - August 26, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Synaptosomal and mitochondrial oxidative damage followed by behavioral impairments in streptozotocin induced diabetes mellitus: restoration by Malvastrum tricuspidatum.
Authors: Solanki I, Parihar P, Shetty R, Parihar MS Abstract Synaptosomal and mitochondrial impairments in the brain of diabetic individual manifest metabolic risk factors that most likely affect the brain functions. Diabetes is associated with the structural and functional alterations of the brain and neuronal loss leading to cognitive and other behavioral impairments, thus reducing the quality of life. The biochemical mechanisms underlying the diabetes-associated behavioral decline are poorly understood. In the present study, we examined the effects of hyperglycemia on the oxidative stress and behaviora...
Source: Cellular and Molecular Biology - August 26, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effect of salinity on gene expression, morphological and biochemical characteristics of stevia rebaudiana Bertoni under in vitro conditions.
Authors: Fallah F, Nokhasi F, Ghaheri M, Kahrizi D, Beheshti Ale Agha A, Ghorbani T, Kazemi E, Ansarypour Z Abstract Stevia rebaudiana Bertoni is a famous medicinal plant for its low calorific value compounds which are named steviol glycosides (SGs) and they are 150-300 times sweeter than sugar. Among various SGs, stevioside and rebaudioside A considered to be the main sweetening compounds.  Soil salinity is one of the most essential stress in the world. Salinity affects the survival and yield of crops. In current study the effects of salinity and osmotic stress caused by different concentration of NaCl (0, 20...
Source: Cellular and Molecular Biology - August 26, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effect of KH2PO4 on gene expression, morphological and biochemical characteristics of stevia rebaudiana Bertoni under in vitro conditions.
Authors: Kahrizi D, Ghari SM, Ghaheri M, Fallah F, Ghorbani T, Beheshti Ale Agha A, Kazemi E, Ansarypour Z Abstract Stevia rebaudiana is one of the most important biologically sourced and low-calorie sweeteners Bertoni that has a lot of steviol glycosides. Tissue culture is the best for propagation of stevia and micro nutrients can affect both morphological traits and steviol glycosides production. Therefore, the effect of different concentrations of KH2PO4on stevia growth factors and gene expression had been studied by tissue culture methods, RT-PCR and HPLC. According the results, bud numbers had increased signif...
Source: Cellular and Molecular Biology - August 26, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Molecular identification of drug resistant mutations to tetracycline in Mycoplasma spp. isolated from patients with multiple sclerosis.
Authors: Naghib M, Kheirkhah B, Mohebbi R, Sadeg L Abstract Bacterial infections play a significant role in causing or intensifying the attacks in MS and there are reports based on the interference of Mycoplasma with a global distribution. Mycoplasma causes autoimmune attacks by imitating the host cell membrane, which is a way of resistance to antibiotics. The purpose of this study was to evaluate the molecular identification of mutations causing resistance to tetracycline in Mycoplasma isolated from MS patients. A total number of 32 cerebrospinal fluid samples and 48 urinal fluid samples were collected from MS pat...
Source: Cellular and Molecular Biology - August 26, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Determination of some heavy metals in Mastacembelus mastacembelus (Banks & Solander, 1794) in terms of public health.
In conclusion the amounts of heavy metals in the flesh of spiny eels were found lower than that recommended by EPA, WHO, FAO and TFC. PMID: 28719337 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - July 20, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

miR-675 promotes disease progression of non-small cell lung cancer via activating NF- κB signaling pathway.
miR-675 promotes disease progression of non-small cell lung cancer via activating NF-κB signaling pathway. Cell Mol Biol (Noisy-le-grand). 2017 May 20;63(5):7-10 Authors: Feng Y, Yang C, Hu D, Wang X, Liu X Abstract Non-small cell lung cancer (NSCLC) is a major type of lung cancer significantly affected human life. Current studies have revealed potential role of miR-675 in the etiology of lung cancer. Therefore, in this study, we aimed to investigate the role of miR-675 in NSCLC cell line. We recruited 92 cases of NSCLC patients from July 2009 to February 2011 in the oncology department. Tumor an...
Source: Cellular and Molecular Biology - July 20, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Modulation of oxidative and glycolytic skeletal muscle fibers Na+/H+ exchanger1 (NHE1) and Na+/HCO3- co-transporter1 (NBC1) genes and proteins expression in type 2 diabetic rat (Streptozotocin + high fat diet) following long term endurance training.
In conclusion, endurance training may improve the capacity of pHi regulation in muscles by lactate-independent pathway. PMID: 28719339 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - July 20, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Sequence comparison of phoR, gyrB, groEL, and cheA genes as phylogenetic markers for distinguishing Bacillus amyloliquefaciens and B. subtilis and for identifying Bacillus strain B29.
Authors: Yu C, Jin J, Meng LQ, Xia HH, Yuan HF, Wang J, Yu DS, Zhao XY, Sha CQ Abstract Given the close genetic relationship between Bacillus amyloliquefaciens and B. subtilis, distinguishing the two solely based on their physiological and biochemical characteristics and 16S rRNA sequences is difficult. Molecular identification was used to discover suitable genes for distinguishing the two bacteria, and to identify the bio-controlling strain B29, due to molecular identification has been paid more and more attention. The similarity of four genes, cheA, gyrB, groEL and phoR, of the two species was compared by the sof...
Source: Cellular and Molecular Biology - July 20, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Label free quantitative proteomics analysis on the cisplatin resistance in ovarian cancer cells.
This study lays a foundation for deciphering the mechanism for drug resistance in ovarian tumor. PMID: 28719341 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - July 20, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

A pilot study for determination of anxiety related SLC6A4 promoter "S" and "L" alleles in healthy Turkish athletes.
A pilot study for determination of anxiety related SLC6A4 promoter "S" and "L" alleles in healthy Turkish athletes. Cell Mol Biol (Noisy-le-grand). 2017 May 20;63(5):29-31 Authors: Corak A, Kapici S, Sercan C, Akkoç O, Ulucan K Abstract We aimed to analyze the allelic distribution of solute carrier family-6 member-4 promoter region in Turkish athletes. Recent studies showed the association of lesser expressing "S" allele with anxiety. Genotype percentages for LL, LS and SS genotypes were found as 46, 35 and 19, respectively. 38% of the males had LL, %38 had LS and 2...
Source: Cellular and Molecular Biology - July 20, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Preparation and properties of polyvinyl alcohol (PVA) and hydroxylapatite (HA) hydrogels for cartilage tissue engineering.
Authors: Yuan F, Ma M, Lu L, Pan Z, Zhou W, Cai J, Luo S, Zeng W, Yin F Abstract A novel bioactive hydrogel for cartilage tissue based on polyvinyl alcohol (PVA) and hydroxylapatite (HA) were prepared, the effects of its component contents on the mechanical properties and microstructure of the hydrogel were investigated. The important properties of the scaffold composites, such as density, porosity, compressive modulus and microstructure were studied and analyzed through various measurements and methods. The biodegradability of hydrogel was evaluated by soaking the samples into artificial degradation solution at bo...
Source: Cellular and Molecular Biology - July 20, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Protective Effect of Edaravone Against A β25-35-Induced Mitochondrial Oxidative Damage in SH-SY5Y Cells.
Protective Effect of Edaravone Against Aβ25-35-Induced Mitochondrial Oxidative Damage in SH-SY5Y Cells. Cell Mol Biol (Noisy-le-grand). 2017 May 20;63(5):36-42 Authors: Zhang GL, Zhang L, Guo YY, Ma ZL, Wang HY, Li T, Liu J, Du Y, Yao L, Li TT, Du JM Abstract Amyloid-β (Aβ)-induced oxidative stress plays an important role in the pathogenesis of Alzheimer's disease (AD). Recent studies show that Aβ accumulation may lead to mitochondrial oxidative damage. In the present study, we investigated the protective effect of edaravone on mitochondrial damage in SH-SY5Y cells treated with A&be...
Source: Cellular and Molecular Biology - July 20, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

H2O2-induced mild stress in relation with in vitro ovine oocyte developmental competence: implications for blastocyst apoptosis and related genes expression.
In this study, in vitro maturation was performed in presence of various concentrations (0, 10, 100, or 1000 µM) of H2O2. The intracellular glutathione (GSH) level, fertilization, cleavage, and blastocyst rates, total cell number, and apoptotic cell number and expression of Bax, Bcl-2, and p53 genes in blastocyst-stage embryos were studied. At 10 μM H2O2 concentration, a higher GSH level was detected in comparison to the other groups while oocytes exposed to 1000 μM H2O2 had the lowest GSH level. Treatment of oocytes with 1000 μM H2O2 decreased the rate of two pronuclei formation as compared with other groups...
Source: Cellular and Molecular Biology - July 20, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research