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Platelet-derived miR-142-3p induces apoptosis of endothelial cells in hypertension.
Authors: Bao H, Yao QP, Huang K, Chen XH, Han Y, Jiang ZL, Gao LZ, Qi YX Abstract The dysfunction of endothelial cells (ECs) plays crucial roles in vascular remodeling during hypertension. Researches suggested that ECs are regulated by the circulating platelets in vivo, which may participate in abnormal EC apoptosis in hypertension. However the molecular mechanism in this process is still unclear. Here we focused on the microRNAs (miRs) in platelets, and detected the potential role and delivery mechanism of platelet-derived miRs in ECs. Using microarray, the differentially expressed profile of miRs between platelet...
Source: Cellular and Molecular Biology - May 11, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effect of matrix rigidity on organ-specific capture of tumor cells by flow.
Authors: Ma P, Shen X, Tan P, Yang L, Liu W Abstract Accumulating evidences have suggested that tumor metastasis exists prominent organ discrepancy. In this progression, the capture of intravascular tumor cells (TCs) to endothelium in distant tissues and organs plays a decisive role in the organ-specific metastasis formation. However, the mechanism of tumor cells preferentially arrest and adhere to endothelial cells (ECs) of target organ still remains elusive. By using the parallel plate flow chamber and the polyacrylamide gels with different matrix stiffness, we here explored the combined effects of matrix ri...
Source: Cellular and Molecular Biology - May 11, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Sphingosine-1-phosphate reduces adhesion of malignant mammary tumor cells MDA-MB-231 to microvessel walls by protecting endothelial surface glycocalyx.
Authors: Zhang L, Zeng M, Fu BM Abstract Sphingosine-1-phosphate (S1P) is a sphingolipid in plasma that plays a critical role in cardiovascular and immune systems. Endothelial surface glycocalyx (ESG) decorating the inner wall of blood vessels is a regulator of multiple vascular functions. To test the hypothesis that S1P can reduce tumor cell adhesion to microvessel walls by protecting the ESG, we quantified the ESG and MDA-MB-231 tumor cell adhesion in the presence and absence of 1μM S1P, and in the presence of the matrix metalloproteinase (MMP) inhibitor in post-capillary venules of rat mesentery. We also meas...
Source: Cellular and Molecular Biology - May 11, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Role of microRNA-124 in cardiomyocyte hypertrophy inducedby angiotensin II.
In conclusion, our study shows that inhibition of miR-124 effectively suppresses AngII-induced myocardial hypertrophy, which is associated with attenuation of ER stress. PMID: 28478799 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - May 11, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

HIF-1 α mediates visfatin-induced CTGF expression in vascular endothelial cells.
In conclusion, the up-regulation of CTGF expression by visfatin might be mediated via HIF-1α -dependent pathway, but not the TGF-β1 pathway in EA.Hy926 cells. PMID: 28478800 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - May 11, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Involvement of TRPC1 in Nampt-induced cardiomyocyte hypertrophy through the activation of ER stress.
Authors: Li J, Wu W, Zhao M, Liu X Abstract Nicotinamide phosphoribosyltransferase (Nampt) is involved in the development of cardiac hypertrophy. Transient receptor potential canonical channel 1 (TRPC1) and endoplasmic reticulum stress (ER stress) are regarded as critical pathways in cardiac hypertrophy. Therefore, we hypothesizedthat TRPC1 might be associated with ER stress in Nampt-induced cardiac hypertrophy. CulturedH9c2cardiomyocyteswereexposed to Namptfor different timesand the expression of markers of cardiomyocyte hypertrophy and ER stress, as well as TRPC1 were detected. Moreover, specific TRPC1-shRNA (sho...
Source: Cellular and Molecular Biology - May 11, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Electroacupuncture ameliorates spatial learning and memory impairment via attenuating NOX2-related oxidative stress in a rat model of Alzheimer's disease induced by A β1-42.
Electroacupuncture ameliorates spatial learning and memory impairment via attenuating NOX2-related oxidative stress in a rat model of Alzheimer's disease induced by Aβ1-42. Cell Mol Biol (Noisy-le-grand). 2017 Apr 29;63(4):38-45 Authors: Wu G, Li L, Li HM, Zeng Y, Wu WC Abstract Alzheimer's disease (AD) is a chronic neurodegenerative disorder characterized by progressive deterioration of cognition and memory, in which oxidative stress has been played a crucial role in the pathology of AD. Electroacupuncture (EA) is a widely used therapy based on traditional acupuncture combined with modern electro...
Source: Cellular and Molecular Biology - May 11, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Peptide 11R-VIVIT stimulates osteoblastogenesis through regulating the expression of nuclear factor of activated T cells cytoplasmic 1.
Authors: Li M, Wang X, Bian Z, Yao W, He Q, Tian F, Zhang J, Zhu L Abstract Osteoporosis is characterized by the imbalance of two relatively independent processes-osteoblastogenesis and osteoclastogenesis. Calcineurin (Cn)/nuclear factor of activated T cells (NFAT)(Cn/NFAT) signaling pathway is involved in these two processes in bone metabolism, but its potential as a target to treat osteoporosis needs to be defined. The aim of this study is to investigate the inhibition of polypeptide 11R-VIVIT onCn/NFAT signaling pathway. Rat calvaria (RC) cells were prepared from experimental model of osteoporosis in rat.11R-VIV...
Source: Cellular and Molecular Biology - May 11, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Connexin 43 enhances oxaliplatin cytotoxicity in colorectal cancer cell lines.
This study suggested that connexin 43 could be considered a molecular target of oxaliplatin activity in colorectal cancer. PMID: 28478804 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - May 11, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Clostridium butyricum partially regulates the development of colitis-associated cancer through miR-200c.
Authors: Xiao Y, Dai X, Li K, Gui G, Liu J, Yang H Abstract Colitis-associated cancer (CAC), one form of colorectal cancer (CRC),is an increasing concern worldwide. Both diagnosis and current therapy are challenging and bottlenecked. The aim of this study is to investigate novel mechanisms by which the therapeutic C. butyricum regulates colitis-induced oncogenesis. Mouse models of CAC were established with 2,4,6-Trinitrobenzenesulfonic acid (TNBS)and azoxymethane (AOM), following by biochemical, clinical and histological analysis. The integrity of epitheliumwas examined by electron microscopy (EM). The epithelial b...
Source: Cellular and Molecular Biology - May 11, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The emerging role of oxidative stress in regulating autophagy: applications of cancer therapy.
Authors: Zhang W, Wan X, Liu Z, Xiao L, Huang H, Liu X Abstract Emerging evidences show that autophagy, as a major cellular adaptive degradation mechanism, is involved in tumorigenesis, cell aging, inflammation and neurodegeneration. It has been reported that multiple stresses including nutrient deprivation, pathogen infection, oxidative stress, endoplasmic reticulum (ER) stress and metabolic stress can influence cellular autophagy, leading to distinct cell fate. Although numerous studies have been employed to elucidate the probable issues, the underlying mechanism of the initiation and maturation of autophagy rema...
Source: Cellular and Molecular Biology - May 11, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Evidence supporting neuroprotective effect of adipose derived stem cells on PC12 cells against oxidative stress induced by H2O2.
Authors: Ghorabi MT, Aliaghaei A, Sadeghi Y, Shaerzadeh F, Rad AA, Mohamadi R, J Ebrahimi M Abstract Adipose-derived stem cells (ADSCs) are a population of cells derived from adipose tissue. ADSCs exhibit multilineage development potential and are able to secrete various factors, which influence adjacent cells. The present study examined the protective effect of ADSC's conditioned media (ADSC-CM) on PC12 cells exposed to H2O2, an oxidative injury model. After isolation, ADSCs were cultured and their osteogenic and adipogenic differentiation confirmed. Then, PC12 cells were co-treated with ADSC-CM and H2O2. Next, th...
Source: Cellular and Molecular Biology - May 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

A proteomics research of apoptosis of leukemia cells induced by arsenic trioxide.
This study could lay the foundation of deciphering mechanisms of HL-60 cell apoptosis and give a new possible target to the clinical application for arsenic trioxide treatment for leukemia. PMID: 28466809 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - May 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Oleanolic acid (OA) regulates inflammation and cellular dedifferentiation of chondrocytes via MAPK signaling pathways.
Authors: Phull AR, Eo SH, Kim SJ Abstract Oleanolic acid (OA) is a bioactive triterpenoid in medicinal plants. It possesses various pharmacological properties, including analgesic, anti-inflammatory, and antitumor effects. The effects of OA in chondrocytes, however, are not well characterized. Here, we used rabbit articular chondrocytes as a cellular model to investigate the effects and regulatory mechanisms of OA on dedifferentiation and pro-inflammation. OA promoted dedifferentiation of chondrocytes by inhibiting type II collagen and pro-inflammatory activity by increasing cyclooxygenase-2 (COX-2) expression. Fur...
Source: Cellular and Molecular Biology - May 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

ARNetMiT R Package: association rules based gene co-expression networks of miRNA targets.
In this study, we introduce ARNetMiT that utilize a hash based association rule algorithm in a novel way to infer the GCNs on miRNA-target genes data. We also present R package of ARNetMiT, which infers and visualizes GCNs of diseases that are selected by users. Our approach assumes miRNAs as transactions and target genes as their items. Support and confidence values are used to prune association rules on miRNA-target genes data to construct support based GCNs (sGCNs) along with support and confidence based GCNs (scGCNs). We use overlap analysis and the topological features for the performance analysis of GCNs. We also inf...
Source: Cellular and Molecular Biology - May 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Molecular analysis of endometrial inflammation in preterm birth.
Authors: Cecati M, Sartini D, Campagna R, Biagini A, Ciavattini A, Emanuelli M, Giannubilo SR Abstract Spontaneous preterm birth (sPTB) represents the 35%-45% of all preterm birth (PTB) cases and its etiology is unknown. We investigated if the expression level of endometrial cytokines and angiogenetic factors is related to the onset of sPTB.Endometrial tissues from non-pregnant women who experienced sPTB and from non-pregnant women who did not experience sPTB were collected and examined for their expression profile. With this aim, the PCR Array analysis was performed and data were confirmed by Real-Time PCR. Differ...
Source: Cellular and Molecular Biology - May 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Association of leukemia inhibitory factor gene polymorphism and in vitro fertilization outcome in a population in northern Iran.
In conclusion, the results of this study indicate that SNP 3951C/T of LIF may not be associated with IVF-ET outcome in this population. Although more studies should be considered with larger number of patients and control subjects to confirm our results. PMID: 28466814 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - May 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The study of CD14+HLA-DR-/low myeloid-drived suppressor cell (MDSC) in peripheral blood of peripheral T-cell lymphoma patients and its biological function.
Authors: Du J, Sun X, Song Y Abstract Peripheral T-cell lymphoma is the generic term of a group of heterogeneous disease in non-Hodgkin's lymphoma. To investigate the quantity of CD14+HLA-DR-/low MDSC and T cell subsets in peripheral blood of peripheral T cell lymphoma (PTCL) patients, and explore the biological functions of CD14+HLA-DR-/low MDSC in peripheral T-cell lymphoma. Flow cytometry was used to determine CD14+HLA-DR-/low MDSC and T cell subsets in peripheral blood of 33 peripheral T-cell lymphoma patients and 23 healthy cases; CD3 +T cells, CD14+HLA-DR-/low MDSC cells and HLA-DR+ cells were selected with m...
Source: Cellular and Molecular Biology - May 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Antioxidant effect of curcumin against exposure to malathion in Cyprinus carpio.
In conclusion, this study demonstrated that malathion caused oxidative stress and negative alterations on the antioxidant enzyme activities of the fish. However, this toxic effect was neutralised by the administration of curcumin. PMID: 28466816 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - May 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Dynamic observation of IL-33 and its receptors in HIV patients who received HAART.
Authors: He B, Zheng L, Zhou H, He Y, Chen Z, Xiao S, Wang H, Ling Y, Zheng Y Abstract The purpose of this study was to observe the changes in serum IL-33 and its soluble receptors, serum sST2 level and ST2L expression in PBMCs of HIV-infected patients receiving highly-active antiretroviral therapy (HAART) for 12 monthes. Fifty-five chronic HIV-1-infected adults were recruited for this study before initiation of HAART. 30 age and gender matched healthy adults were recruited as control. Blood was obtained from each patient at baseline (0 month) and 12 months after initiation of highly active antiretroviral therapy(H...
Source: Cellular and Molecular Biology - May 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Evaluation of relationship between aerobic fitness level and range of isocapnic buffering periods during incremental exercise test.
Authors: Algul S, Ozcelik O, Yilmaz B Abstract The purpose of this study is to examine the relationship between the amount of O2 uptake (VO2) in the range of isocapnic buffering (ICB) periods and aerobic fitness levels of subjects with different exercise tolerance levels. A total of 50 young male subjects (20.8±0.4 years) performed an incremental exercise test using a cycle ergometer to determinetheir anaerobic threshold (AT), respiratory compensation point (RCP) and maximal exercise capacity (Wmax). The ICB period is defined as the region between AT and RCP. Pulmonary gas exchange parameters were measured b...
Source: Cellular and Molecular Biology - May 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Familial Mediterranean Fever: Observations from a pilot gene expression microarray analysis study.
Authors: Mahfouz R, Kreidieh K, Khalek RA, Yazbek S Abstract Familial Mediterranean Fever (FMF) is an autosomal recessive autoinflammatory disease affecting people of Mediterranean ancestry. The disease is caused by mutations in the MEFV gene located on chromosome 16p13.3. The aim of this pilot study was to assess global gene expression and identify genes and pathways involved in FMF that could be downstream to MEFV mutations or could be novel involved. EDTA blood samples were collected from 14 patients showing FMF-like symptoms and age-matched to 7 controls showing healthy conditions. Microarray was used to assess...
Source: Cellular and Molecular Biology - May 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Association between BANK1 polymorphisms and susceptibility to autoimmune diseases: A meta-analysis.
This study aimed to explore whether BANK1 polymorphisms are associated with susceptibility to autoimmune diseases. We conducted a meta-analysis on the associations between the BANK1 rs10516487, rs3733197, and rs17266594 polymorphisms and autoimmune diseases. Twenty-two articles with a total of 22,684 patients and 36,437 controls were included in the meta-analysis. Meta-analysis revealed a significant association between autoimmune diseases and the BANK1 rs10516487 T allele (OR = 1.161, 95% CI = 1.092-1.275, p = 1.9 × 10-6, heterogeneity p
Source: Cellular and Molecular Biology - May 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Long non-codingRNA (lncRNA) TUG1 and the prognosis of cancer: a meta-analysis.
In conclusion, this meta-analysis study indicated that TUG1 might associate with the OS of osteosarcoma and digestive system's tumors. PMID: 28466821 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - May 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The effects of CDKN2A rs3731249, rs11515, and rs3088440 polymorphisms on cancer risk.
Authors: Dong Y, Wang X, Yang YW, Liu YJ Abstract Many studies have been carried out to examine whether there is an association between CDKN2A polymorphisms and cancer risk, but the results from these studies were controversial. In the present study, we performed a meta-analysis to clarify whether there is an association of CDKN2A polymorphisms and cancer risk. Published reports were searched in PubMed and Google Scholar. ORs with 95% CIs were calculated in the dominant models. Twenty six case-control studies that met the inclusion criteria were included in the final meta-analysis. Overall, we found that rs3731249,...
Source: Cellular and Molecular Biology - May 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The effects of melatonin administration on KCNQ and KCNH2 gene expressions and QTc interval in pinealectomised rats.
Authors: Ovali MA, Uzun M Abstract Melatonin is a hormone secreted from the pineal gland and has different cardiovascular effects. KCNQ genes expressed in aorta related with vascu- lar tone and KCNH2 gene characterised in left ventricle associated with QT duration. The aim of this study was to investigate the effects of melatonin on the regulation of the blood pressure and the relationships between the expressions of aorta KCNQ1-5, left ventricle KCNH2 genes and the QTc interval. For that purpose, 42 male adult Sprague-Dawley rats were devided into six groups; SHAM, SHAM+L-NAME, PLT, PLT+L-NAME, PLT+MEL and PLT+L-N...
Source: Cellular and Molecular Biology - May 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The effects of Nobiletin, Hesperetin, and Letrozole in a combination on the activity and expression of aromatase in breast cancer cells.
In this study, aromatase enzyme activity based on the conversion of androgen substrate testosterone to 17β-Estradiol was determined. Estradiol concentrations were measured using an electrochemiluminescence immunoassay. CYP19 gene expression was determined by quantitative real-time PCR. Our findings demonstrated that none of combinations including LET+NOB, LET+HES, LET+NOB+HES, and NOB+HES had no significant effects on aromatase activity and expression. The present study showed for the first time that the combination of HES, NOB, and LET had no effects on activity and expression of aromatase in MCF-7 breast cancer cell...
Source: Cellular and Molecular Biology - April 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The link between intergenic distances and controls exerted on the transcriptional regulation; an inferential approach.
Authors: Ahmadi M, Salehi B Abstract The position of genes along the genome is an important evolutionary factor for organizing gene regulation. Hence, transcriptional regulatory network have been studied much more extensively from gene distributions rather than other viewpoints. The systematics of intergenic distances, therefore, should be taken into account as an important source of information on the controls exerted on gene expression by various biological mechanisms. Here we study a collection of features including, intergenic and interoperonic distances, distances between isolated genes, distances between regu...
Source: Cellular and Molecular Biology - April 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Methyl thiophanate-induced toxicity in liver and kidney of adult rats: a biochemical, molecular and histopathological approach.
Authors: Feki A, Ben Saad H, Jaballi I, Magne C, Boudawara O, Zeghal KM, Hakim A, Ben Ali Y, Ben Amara I Abstract The aim of this study was to elucidate the redox effects of Thiophanate methyl (MT) in the rat liver and kidney. Our results showed, after 3 days of MT injection (700 mg/kg), an increase in malondialdehyde (MDA), hydrogen peroxide and advanced oxidation protein products levels. Glutathione peroxidase and superoxide dismutase activities were also remarkably increased in the liver but decrease in the kidney. Glutathione and vitamin C values were significantly reduced. The changes in biochemical parameters...
Source: Cellular and Molecular Biology - April 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The G-protein alpha-subunit gene CGA1 is involved in regulation of resistance to heat and osmotic stress in Chlamydomonas reinhardtii.
Authors: Lee CS, Ahn W, Choi YE Abstract In eukaryotic cells, many important functions of specific G-proteins have been identified, but microalgal G-proteins are poorly studied. In this work, we characterized a gene (CGA1) encoding the G-protein α-subunit in Chlamydomonas reinhardtii. Independent knockdown mutants of CGA1 were generated via RNA interference (RNAi). CGA1 expression levels were consistently and significantly reduced in both independent CGA1 mutant cell lines (cga1). Both cga1 mutants had a higher survival rate at 35°C in comparison with the wild type. This stronger resistance of the cga1 mu...
Source: Cellular and Molecular Biology - April 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

miRNA and mRNA expression profiling in rat brain following alcohol dependence and withdrawal.
In conclusion, consistent and integrated variations in miRNA expression and in their focus mRNAs in rat brain were noted after alcohol exposure and withdrawal. Besides, understanding the molecular mechanisms of alcohol abuse will no doubt guide to development of significant cure methods for addiction. We are of the opinion that our findings may shed light on classification of novel biomarkers. PMID: 28364783 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - April 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

TTY2 genes deletions as genetic risk factor of male infertility.
This study was performed on 45 infertile males with idiopathic azoospermia without any AZF micro deletions (group A), 33 infertile males with azoospermia which do not screened for AZF micro deletions (group B) and 65 fertile males (group C), from October 2013 to April 2015 in west of Iran. Polymerase chain reaction (PCR) method was used for detection of TTY2L12A and TTY2L2A gene deletions in studied groups. No deletions were detected in normal fertile males of group C. 1 out of 45 azoospermic males of group A (2.22%) and 3 out of 33 azoospermic males of group B (9.09%) had TTY2L2A deletion (p= 0.409 and p= 0.036 respective...
Source: Cellular and Molecular Biology - April 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Stable silencing of IGF1R using Lentiviral-mediated shRNA in HEK293T cells.
Authors: Nasehi L, Ghahremani MH, Yavari K, Hadjati J, Fallah A, Abdolhossein Zadeh B, Saltanatpour Z Abstract Insulin-like growth factors are among the peptide mitogens that regulate cell proliferation and differentiation as well as mediator of antiapoptotic signals. The imbalance between the expression and activities of these molecules may lead to malignancy in cells. Evidences have suggested the insulin-like growth factor 1 receptor (IGF-1R) signaling pathway as a therapeutic target in the management and treatment of cancer. In this present study, we have generated silencing stable clones of HEK cells using six ...
Source: Cellular and Molecular Biology - April 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Dandelion-enriched diet of mothers alleviates lead-induced damages in liver of newborn rats.
This study evaluated the hepatic toxic effects of prenatal exposure to lead in rats and the possible protective effect of dandelion (Taraxacum officinale) added to the diet. Female rats were given a normal diet (control) or a diet enriched with dandelion (treated). In addition, lead acetate was administered to half of the rats through drinking water from the 5th day of gestation until the 14th day postpartum. Lead toxicity was evaluated in their offspring by measuring body and liver weights, plasma biochemical parameters, liver damage, as well as protein content and activities of antioxidant enzymes in the liver tissues. L...
Source: Cellular and Molecular Biology - April 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

TGF ß3 / SfaN1 gene variant and the risk factor of nonsyndromic cleft palate only among Indonesian patients.
In conclusion, TGFβ3 gene / SfaN1 polymorphism can be considered as the risk factor associated with NS CPO in Indonesian patients. PMID: 28364787 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - April 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The clinical research of Thinprep Cytology Test (TCT) combined with HPV-DNA detection in screening cervical cancer.
Authors: Liu Y, Zhang L, Zhao G, Che L, Zhang H, Fang J Abstract Our objective is to explore the clinical value of thinprep cytologic test (TCT) combined with HPV-DNA detection in screening cervical cancer. 420 cervical cancer patients admitted in our hospital between April, 2011-April, 2014 were selected. All patients received TCT and HPV-DNA detection, and cervical tissue biopsy was used to confirm the diagnosis. TCT screening results showed that there were 175 patients were >ASCUS and the positive rate was 41.7%, histopathological screening showed that there were 199 patients were ≥cervical intraepithe...
Source: Cellular and Molecular Biology - April 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Ghrelin did not change coronary angiogenesis in diet-induced obese mice.
Authors: Khazaei M, Tahergorabi Z Abstract Ghrelin is a 28 amino acids peptide that initially was recognized as an endogenous ligand for growth hormone secretagogue receptor (GHSR). Recently, a number of studies demonstrated that ghrelin is a cardiovascular hormone with a series cardiovascular effect. The main objective of this study was to investigate the effect of systemic ghrelin administration on angiogenesis in the heart and its correlation with serum leptin levels in normal and diet-induced obese mice. 24 male C57BL/6 mice were randomly divided into four groups: normal diet (ND) or control, ND+ghrelin, high-f...
Source: Cellular and Molecular Biology - April 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The effects of melatonin on oxidative stress and prevention of primordial follicle loss via activation of mTOR pathway in the rat ovary.
This study indicated that melatonin may have a significant protective effect on primordial follicles and increase the expression of mTOR and downstream components in rat ovary. Melatonin treatment may have a beneficial effect on fertility. PMID: 28364790 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - April 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effect of nitrogen sources on some morphological characteristics of in vitro stevia rebaudiana Bertoni.
Authors: Akbari F, Arminian A, Kahrizi D, Fazeli A Abstract Stevia rebaudiana Bertoni belongs to Asteraceae family that leaves 200-300 times sweeter than sugar. Low seed fertility is one of the most important problems in Stevia production. So, Plant tissue culture is an efficient method for mass propagation of Stevia. In this research, we studied the effect of various concentrations of nitrogen on some morphological traits of stevia under in vitro conditions. We used axillary nodes as explants and they were cultured on Murashige and Skoog (MS) medium containing inorganic nitrogen sources i.e. NH4NO3(0, 825 and 1650...
Source: Cellular and Molecular Biology - April 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Identification of bacteria using volatile organic compounds.
Authors: Karami N, Karimi A, Aliahmadi A, Mirzajan F, Rezadoost H, Ghassempour A, Fallah F Abstract The rapid diagnosis of respiratory infections has always been an important goal for medical professionals, because rapid and accurate diagnosis leads to proper and timely treatment, and consequently, reduces the costs of incorrect and long-term treatments, and antibiotic resistance. The present study was conducted with the aim of detecting volatile organic compounds (VOCs) in three bacteria: Pseudomonas aeruginosa, Acinetobacter baumannii, Klebsiella pneumoniae. Headspace of the studied bacteria, after separately cul...
Source: Cellular and Molecular Biology - April 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Signaling networks in TMPRSS2-ERG positive prostate cancers: Do we need a Pied Piper or sharpshooter to deal with "at large" fused oncoprotein.
Signaling networks in TMPRSS2-ERG positive prostate cancers: Do we need a Pied Piper or sharpshooter to deal with "at large" fused oncoprotein. Cell Mol Biol (Noisy-le-grand). 2017 Feb 28;63(2):1-8 Authors: Lin X, Qureshi MZ, Romero MA, Yaylim I, Arif S, Ucak I, Fayyaz S, Farooqi AA, Mansoor Q, Ismail M Abstract Overwhelmingly increasing scientific evidence has provided near complete resolution of prostate cancer landscape and it is now more understandable that wide ranging factors underlies its development and progression. Increasingly it is being realized that genetic/epigenetic factors, In...
Source: Cellular and Molecular Biology - April 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Review of new insights into antimicrobial agents.
Authors: Dehghan Esmatabadi MJ, Bozorgmehr A, Hajjari SN, Sadat Sombolestani A, Malekshahi ZV, Sadeghizadeh M Abstract People have known the bacteria and have used various ways to deal with them, from a long time ago. Perhaps, natural antibiotics with have been the first step in fighting against pathogens. However, several factors, such as dealing with unfamiliar bacteria or emergence of drug-resistant species, have motivated us to discover new antibiotics or  even change previous types. In this regard, a variety of natural and synthetic antibiotics with different origins, mechanism of action, structures and f...
Source: Cellular and Molecular Biology - April 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The significance of DNA methylation profile in metastasis-related genes for the progression of colorectal cancer.
Authors: Tokarz P, Pawlowska E, Bialkowska-Warzecha J, Blasiak J Abstract DNA methylation, an epigenetic modification plays a role in the pathogenesis of colorectal cancer (CRC). CRC cases, both sporadic and familial, are often characterized by abnormal pattern of the cytosine methylation in CpG dinucleotides in regulatory regions of genes important for cancer transformation. Also genes mutated in CRC can have their epigenetic pattern altered and we suggest that changes in DNA methylation array can be important for CRC metastatic potential ‒ the main reason of CRC-associated mortality. These genes are: KRAS, gene...
Source: Cellular and Molecular Biology - April 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Comparison of two DNA extraction protocols from leave samples of Cotinus coggygria, Citrus sinensis and Genus juglans.
Authors: Fallah F, Minaei Chenar H, Amiri H, Omodipour S, Shirbande Ghods F, Kahrizi D, Sohrabi M, Ghorbani T, Kazemi E Abstract High quality DNA is essential for molecular research. Secondary metabolites can affect the quantity and quality DNA. In current research two DNA isolation methods including CTAB and Delaporta (protocols 1 & 2 respectively) were applied in three leave samples from Cotinus coggygria, Citrus sinensis and Genus juglans that their leaves are rich of secondary metabolites. We successfully isolated DNA from C. coggygria, C. sinensis and Genus Juglans using the two protocols described abo...
Source: Cellular and Molecular Biology - April 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Response of antioxidant system to formalin in the whole body of rainbow trout, Oncorhynchus mykiss.
Authors: İspir U, Kirici M, Yonar ME, Mişe Yonar S Abstract Formalin bath treatments are widely used to control of parasitic infections in aquaculture. However, effects of formaldehyde on the lipid peroxidation and antioxidant system were not still need elucidation. Fish were exposed to formalin at doses of 50, 100 and 200 ppm for 1 h. Fish were then left to depurate for 24 h in formalin-free water. At the end of the test, whole bodies were isolated and homogenized to measured malondialdehyde (MDA) and reduced glutathione  (GSH) levels and catalase (CAT) and glutathione peroxidase (GPx ) activities. Results ...
Source: Cellular and Molecular Biology - February 27, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

β-Catenin overexpression causes an increase in inflammatory cytokines and NF-κB activation in cardiomyocytes.
In this study, roles of β-catenin in inflammation in cardiomyocytes were investigated. 10 samples from hearts of patients with acute infarction and 10 from normal ones were collected in order to access roles of β-catenin in cardiomyocytes. H9c2 cardiomyoblasts and primary neonatal rat cardiomyocytes were transfected with porcine cytomegalovirus (pCMV)-β-catenin plasmid in order to overexpress β-catenin. Protein level of β-catenin protein was increased in human acute infarction tissues compared to ones from normal patients. The transcription factor had increased nuclear localization in cardiomyocyte...
Source: Cellular and Molecular Biology - February 27, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Induction of cellular proliferation in a human astrocytoma cell line by a Trypanosoma cruzi-derived antigen: a mechanism of pathogenesis?
Authors: Duran-Rehbein GA, Vargas-Zambrano JC, Cuéllar A, Puerta CJ, Gonzalez JM Abstract Trypanosoma cruzi can compromise the human central nervous system (CNS) during acute infection or reactivation in immune-suppressed hosts. Astrocytes have been identified as targets of T. cruzi's CNS infection in humans. Despite a high degree of parasitism and cellular lysis by T. cruzi in vitro the number of astrocytoma cells did not change when compared to uninfected cultures. This work evaluated cellular proliferation, changes in Major Histocompatibility Complex (MHC) expression as a reflection of antigen processing,...
Source: Cellular and Molecular Biology - February 27, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

CagA-positive and CagA-negative Helicobacter pylori strains differentially affect the expression of micro RNAs 21, 92a, 155 and 663 in human umbilical vein endothelial cells.
Authors: Kalani M, Hodjati H, GhamarTalepoor A, Samsami Dehaghani A, Doroudchi M Abstract Given that the basic mechanism of the effect of Helicobacter (H.) pylori in the induction of atherosclerosis remains unknown and regarding the regulatory role of micro RNAs (miRNAs) in endothelial cell (EC) functions, we aimed to investigate the effect of H. pylori on the expression of miRNAs involved in atherosclerosis (atheromiRs) and their correlation with apoptosis in human umbilical vein EC (HUVEC). HUVECs were treated with different cytotoxin associated gene A (CagA) positive and negative H. pylori derived products, then...
Source: Cellular and Molecular Biology - February 27, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effect of SOX2 on osteogenic differentiation of dental pulp stem cells.
Authors: Yuan J, Liu X, Chen Y, Zhao Y, Liu P, Zhao L, Han W Abstract To explore the effect of SOX2 on osteogenic differentiation of dental pulp stem cells (DPSCs) and to develop a new method for facilitating osteogenic differentiation of DPSCs. SOX2-overexpressing human DPSCs (DPSCs-SOX2) were established through retrovirus infection. Differentiation was induced with osteogenic differentiation medium and further evaluated by alkaline phosphatase (ALP) staining and qPCR. Following the differentiation procedure, the mechanism of SOX2 on osteogenic differentiation of DPSCs was analyzed using genome RNA microarray and...
Source: Cellular and Molecular Biology - February 27, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Beneficial effects of deep sea fish oil on diabetic mice neurological injury.
In conclusion, deep sea fish oil could be used as auxiliary health care products, which plays important role in preventing and treating implications of nerve lesion impairment induced by diabetes mellitus. PMID: 28234624 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - February 27, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research