Analysis of potential interactions between vitamins D and C using gene expression profiles from mouse models.
This study is to investigate how VD and VC interact at molecular level using whole genome expression profiles from spleens of 111 mouse strains and livers from 40 mouse strains. We first identified the genes that are closely correlated to vitamin D receptor (Vdr) and gulonolactone oxidase (Lgo). We next analyzed the potential molecular pathways of Vdrand Lgo correlated genes and examined how these two sets of pathways are connected in liver and spleen. Our results indicated that Vdrand Lgo associate to distinct groups of genes. While most of genes are not the same, a few of them are associated to both Vdrand Lgo.  Our...
Source: Cellular and Molecular Biology - December 8, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Human haematological and epithelial tumor-derived cell lines express distinct patterns of onco-microRNAs.
In this study, we analysed differences in miRNA expression profile between haematological and epithelial tumor-derived cell lines and explored their role in definying different cancer cells phenotypes. Cancer Focus microRNA PCR Panel was used to analyze eighty-four oncomiRNAs in two human haematological (K562 and HL-60) and in two epithelial (H460 and MCF-7) cancer cell lines. Bioinformatic tools were used to identify miRNA-specific signatures and to discover potentially deregulated pathways. Our analysis led to the identification of i) a large repertoire of miRNAs commonly expressed in the four cell lines, including two e...
Source: Cellular and Molecular Biology - December 8, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Bioactive flavones isolated from Tunisian Artemisia campestris L. Leaves.
Authors: Metoui R, Bouajila J, Znati M, Cazaux S, Neffati M, Akrout A Abstract Four flavones were isolated from dried leaves of Artemisia campestris L. 2',4',5,7-tetrahydroxy-5',6-dimethoxyflavone, eupatilin and dimethoxycentaureidin were reported for the first time in this species whereas cirsiliol was previously identified but it was isolated for the first time. Their structures were elucidated by spectroscopic methods, including 1D and 2D NMR experiments and mass spectrometry analysis. In addition, all isolated flavones were evaluated for their antioxidant, anti-inflammatory, anti-superoxide dismutase, anti-xant...
Source: Cellular and Molecular Biology - December 8, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Genetic characterization and assessment of demographic bottleneck in Caspian horse population.
This study determined the efficiency of microsatellite markers for conservation plans and breeding strategies in Caspian horse population. For this purpose, 120 Caspian horse samples including 95 adults and 25 foals were genotyped by using seventeen microsatellite markers recommended by ISAG. The number of allele per locus varied from 5 (HMS01 and HTG07) to 9 (HTG10) with an average value of 7.41. The observed heterozygosity and the expected heterozygosity ranged 0.505-0.831 (mean 0.684), from 0.615-0.835 (mean 0.748) respectively. PIC value ranged from 0.716 (HMS01) to 0.834 (AHT04) with average 0.787. The total exclusion...
Source: Cellular and Molecular Biology - December 8, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Curcumin induce DNA damage and apoptosis through generation of reactive oxygen species and reducing mitochondrial membrane potential in melanoma cancer cells.
Authors: Kocyigit A, Guler EM Abstract Melanoma is the most malignant skin cancer. Curcumin has shown to have therapeutic effects when used in the treatment of malignant diseases. However, the precise molecular mechanisms of its action are not fully elucidated. In this research, we hypothesized that reactive oxygen species (ROS) play a key role in curcumin induced DNA damage, apoptosis and cell dead. To test our hypothesis, cytotoxic, genotoxic, apoptotic, ROS generating and mitochondrial membrane potential (MMP) of curcumin on mouse melanoma cancer cells (B16-F10) and fibroblastic normal cells (L-929) were in...
Source: Cellular and Molecular Biology - December 8, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Potential biomarkers for the diagnosis of respiratory tract infection and lungs cancer.
Authors: Khan K, Aslam MA, Zahra FT, Basheer H, Bilal M, Sumrin A Abstract Major hurdle faced by many physicians in treating various respiratory tract infections and lung carcinomas is their late or mis-diagnosis. In most respiratory tract infections the manner of infection is not completely understood. Similarly, various lung carcinomas are diagnosed at advance stages at which not only the treatment possibilities are narrowed but the chances of survival are also reduced. So, for the sake of better treatment, the quick and improved diagnostic strategies are suggested. Protein biomarkers fully fit the description in...
Source: Cellular and Molecular Biology - December 8, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Complexity of phenotypes induced by p.Asn1303Lys-CFTR correlates with difficulty to rescue and activate this protein.
Authors: Farhat R, El-Seedy A, Norez C, Talbot H, Pasquet MC, Adolphe C, Kitzis A, Ladevèze V Abstract Cystic Fibrosis is the most common recessive autosomal rare disease found in Caucasian. It is caused by mutations on the Cystic Fibrosis Transmembrane Conductance Regulator gene (CFTR) that encodes for a protein located on the apical membrane of epithelial cells. c.3909C>G (p.Asn1303Lys) is one of the most common worldwide mutations located in nucleotide binding domain 2. The effect of the p.Asn1303Lys mutation on misprocessing was studied by immunofluorescence and western blotting analysis in presen...
Source: Cellular and Molecular Biology - December 8, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Promoter motifs required for c-mpl gene expression induced by thrombopoietin in CMK cells.
Authors: Sunohara M, Sato I, Morikawa S Abstract Thrombopoietin (TPO) and its receptor, c-Mpl, are the central regulators of megakaryocyte development and platelet production and are also crucial to regulate megakaryocytopoiesis. TPO remarkably elevated c-mpl promoter activity, while the protein kinase C (PKC) inhibitors, GF109203, H7 and Calphostin C, clearly reduced the steady level of its promoter activity.  In the present study, motifs crucial for c-mpl promoter activity induced by TPO treatment has been analyzed using a human megakaryoblastic cell line, CMK. Destruction of the -107Sp1 and the -57Sp1 sites...
Source: Cellular and Molecular Biology - December 8, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Development of PCR primer systems for amplification of 16S-rDNA to detect of Thiobacillus spp.
Authors: Beheshti Ale Agha A, Kahrizi D, Ahmadvand A, Bashiri H, Fakhri R Abstract Thiobacillus is a genus of Gram-negative, rod-shaped and autotrophic Betaproteobacteria. They catalyze the dissimilatory oxidation of elemental sulfur and reduced inorganic sulfur compounds. Whereas more than 30 species have been known in this genus, most were never reliably or effectively published. The rest were either reclassified into Thiomonas, Paracoccus, Starkeya, Sulfuriferula, Halothiobacillus, Thermithiobacillus or Acidithiobacillus, were lost from culture. Most of Thiobacillus species are obligate autotrophs via elementary...
Source: Cellular and Molecular Biology - December 8, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Association between vitamin D receptor BsmI gene polymorphism and periodontitis: a meta-analysis in a single ethnic group.
In conclusion, our meta-analysis showed that VDR BsmI polymorphism was associated with the decreased risk of periodontitis in Chinese individuals from South China, and further studies in other ethic groups are required for definite conclusions. PMID: 29208185 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - December 8, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Association between platelet glycoprotein Ia C807T gene polymorphism and ischemic stroke: a meta-analysis in a separate ethnic group.
Authors: Huang XY, Fu WJ, Mei ZZ, Yu YL, Huang YH, Lin H, Chen JJ, Wang MX, Guan SB, Fang HW Abstract Many studies have been examined the association of platelet glycoprotein (GP) Ia C807T polymorphism with ischemic stroke (IS) susceptibility. However, the results of these studies are inconsistent. To further assess the effects of GP Ia C807T polymorphism on the risk of IS, a meta-analysis was performed in a separate ethnic group. Relevant studies were identified using PubMed and Chinese databases through January 2017. Pooled odds ratios (ORs) and 95% confidence intervals (CIs) were used to assess the strength of t...
Source: Cellular and Molecular Biology - December 8, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

A meta-analysis study of the association between EGFR rs2252586 mutation and the risk of glioma.
In conclusion, this meta-analysis found that EGFR rs2252586 mutation was significantly associated with glioma risk. PMID: 29208187 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - December 8, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The antiosteoporosis effect of icariin in ovariectomized rats: a systematic review and meta-analysis.
Authors: Liu Y, Zuo H, Liu X, Xiong J, Pei X Abstract Herba Epimedii (Yinyanghuo or Horny Goat Weed) is a traditional Chinese medicine widely used in treating osteoporosis. As the major active component, icariin is intensively investigated in the prevention and treatment of osteoporosis in ovariectomized rats. However, misleading conclusions can be generated in animal studies with various experimental designs. Therefore, a systematic review and meta-analysis was performed to evaluate the efficacy of icariin against osteoporosis in ovariectomized rats. PubMed, EMBASE and Chinese National Knowledge Infrastructure dat...
Source: Cellular and Molecular Biology - December 8, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Enlightenment and lesson from the successful treatment of acute superior mesenteric artery embolism.
Authors: Wang B, Sun R, Chen W, Li T Abstract  Despite recent advances, treatment of acute superior mesenteric artery embolism (SMAE) in emergency is still very difficult. The comparative rarity, the difficulty of early diagnosis, and the extremely high mortality of SMAE give us the sufficient reasons to report our successful experience in curing a patient with acute SMAE. In the present case, the patient was diagnosed early without evidence of intestinal necrosis. The diagnosis of SMAE was verified by computed tomography angiography (CTA). A comprehensive treatment was immediately employed including a combina...
Source: Cellular and Molecular Biology - December 8, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Metabolic and molecular responses to calcium soap of fish oil fed to ewes during peripartal period.
Authors: Sheibani S, Khadem AA, Mohammadi-Sangcheshmeh A, Alamouti AA, Norouzian MA Abstract It has been shown that n-3 long chain fatty acids (n-3 LCFA) are involved in energy/lipid metabolisms, reproductive parameters, and molecular regulations leading to maintained homeostasis. We hypothesized that supplementation of peripartal diets with fish oil (FO), as a source of n-3 LCFA, could improve energy balance and modulate metabolic pressure in a sheep model. Prepartum ewes (n = 24) were fed control (CON) or  calcium soap of fish oil (FO) supplemented-diet from four weeks before until three weeks after parturat...
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Induced dedifferentiation of barley (Hordeum vulgare L.) embryonic cells and its relationship with agronomic traits.
This study was carried out to investigate the response of 42 Iranian and European barley (Hordeum vulgare L.) cultivars to induced dedifferentiation of embryonic cells via immature embryo culture and understand the relationship between embryo culture characters and agronomic traits. The cultivars were evaluated for dedifferentiation of embryonic cells or callus induction from immature embryo culture based on a completely randomized design with unequal replication. Immature embryos were placed scutellum down on cell dedifferentiation medium based on MS and supplemented with 2.5 mg/l 2,4-D. The developed calli were transferr...
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effect of pomegranate (Punica granatum L.) juice on kidney, liver, heart and testis histopathological changes, and the tissues lipid peroxidation and antioxidant status in lead acetate-treated rats.
This study investigated the protective potential of PJ against lead-induced oxidative stress. A total of forty adult male Sprague Dawley rats were divided into four experimental groups. The animals were fed a standard pellet diet and tap water ad libitum. The rats were divided into four groups (n=10 for each group): control, lead asetat (2000 ppm), low-treated PJ- a daily dose of 2.000 ppm lead plus 30µl pomegranate juice (included 1.050 µmol total polyphenols, gallic acid equivalent), and high-treated PJ- a daily dose of 2.000 ppm lead plus 60µl pomegranate juice (included 2.100 µmol total polyphen...
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

HMGCR and ApoE mutations may cause different responses to lipid lowering statin therapy.
The objective of this study was to investigate whether common variations in HMG-CoA Reductase(HMGCR) and Apolipoprotein E (ApoE) genes involved in lipid and statin metabolism modify the effect of statins on serum lipid and lipoprotein concentrations in CAD patients.A hundred  CAD patients were enrolled into the study. At the beginning of the study biochemical measurements were performed to determine the baseline levels performed. Patients were treated with 40 mg atorvastatin for 2 months and biochemical measurements were repeated. According to the post-treatment, LDL-c levels,  patients were divided into 2 groups...
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Cardioprotective effect of Pycnogenol in ischemic-reperfusion injury (IRI) in rats.
Authors: Xiao P, Zhang K, Tao Z, Liu N, Ge B Abstract Oxidative stress plays a critical task in the biochemical and pathological alteration linked with myocardial ischemic-reperfusion injury (IRI). This warrants identifying agents with a potential for preventing such damage in an effective way. A novel plant based product, Pycnogenol, obtained from the French maritime pine (Pinus pinaster ssp. atlantica) bark extract was known for its tremendous antioxidant potential (both in vivo, in vitro). It was able to attenuate the symptoms of immune dysfunction through restoring a cellular antioxidant status in low micronutr...
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Hyperandrogenism stimulates inflammation and promote apoptosis of cumulus cells.
Authors: Xu F, Liu R, Cao X Abstract To explore roles of androgen and cytokines such as IL-1, IL-6 and TNF-α in PCOS. Cumulus cells were isolated from rat COCs and divided into two sets of experiments. Set 1 includes 4 groups: Control group, IL-1 group (final concentration at 5 nM), IL-6 group (final concentration at 5nM) and TNF-α group (final concentration at 5nM). Set 2 also include 4 groups: Control group, Androgen group 1 (final concentration at 1 nM), Androgen group 2 (final concentration at 10 nM) and Androgen group 3 (final concentration at 100 nM). Androgen, at final concentration of 100 nM, ca...
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Application of unilateral multiple channels approach in percutaneous vertebroplasty for osteoporotic vertebral fractures.
This study aimed to investigate the application valuable of percutaneous vertebroplasty (PVP) with unilateral multiple channels approach in osteoporotic vertebral fractures patients. A retrospective review was conducted on 685 consecutive patients with osteoporotic vertebral fracture from March 2003 to October 2012. Among them, 82 cases were given PVP procedure by unilateral multiple channels approach. The timing of surgery, bone cements injection, and complications were analyzed. By using the X-ray film, the distribution of cement was detected and scored. The visual analogue scale (VAS) score and Oswestry disability index...
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Overexpression of microRNA-133b reduces myocardial injuries in children with viral myocarditis by targeting Rab27B gene.
Authors: Zhang Y, Sun L, Sun H, Liu X, Luo X, Li C, Sun D, Li T Abstract The present study is to measure the expression of microRNA (miRNA or miR)-133b in circulating blood of children with viral myocarditis before and after drug treatment, and to investigate its relationship with the severity of myocardial lesions. A total of 36 children patients with viral myocarditis who received treatments at our hospital between June 2014 and June 2016 were enrolled in the present study, including 21 boys and 15 girls (age range, 9 months - 16 years).Quantitative real-time polymerase chain reaction was used to determine the ex...
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Investigation of genotoxic effects of doripenem using cytogenetic and molecular methods.
In this study, according to the obtained results, it was determined that DRP failed to show any genotoxic risk at the therapeutic doses. This result also indicates that DRP could be a reliable antibiotics according to its rapid metabolism. PMID: 29096747 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Organic chromium modifies the expression of orexin and glucose transporters of ovarian in heat-stressed laying hens.
In conclusion, CrPic and CrHis showed supported the relief and treatment of stress complications. PMID: 29096748 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

A bioinformatics approach for identification of miR-100 targets implicated in breast cancer.
Authors: Shamsi R, Seifi-Alan M, Behmanesh A, Omrani MD, Mirfakhraie R, Ghafouri-Fard S Abstract MicroRNAs (miRNAs) are small endogenous non-coding RNAs with principal roles in regulation of protein expression via translation repression and mRNA degradation. Based on these roles they are implicated in tumourigenesis processes as well. Among them is miR-100 which can exert both tumor suppressor and oncogenic functions in various cancer types. In breast cancer, it has been shown to affect apoptosis, epithelial-mesenchymal transition as well as tumor-related signaling pathways. In the present study, we introduce a nov...
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Clinical efficacy and mechanism of lymphoplasma exchange in the treatment of Guillain-Barre syndrome.
Authors: Luo MC, Wang WF, Yin WF, Li Y, Li BJ, Duan WW, Zeng QM, Luo YB, Yang XS, Yang H Abstract Guillain-Barre syndrome (GBS) is an autoimmune disease of the nervous system and is the most common acute polyneuropathy. Both cellular and humoral immunity are believed to be involved in the pathogenesis of GBS, and various types of activated CD4+ T cells are thought to orchestrate the onset and progression of GBS. Lymphoplasma exchange (LPE) filtering out activated lymphocytes while exchanging plasma has been used for GBS treatment for years. However the treatment is still not yet optimal. In order to assess the effi...
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

LPS-induced Src family kinases activity mediates IL-10 production through activation of STAT3 in peripheral blood mononuclear cells of patients with Beh çet's Disease.
LPS-induced Src family kinases activity mediates IL-10 production through activation of STAT3 in peripheral blood mononuclear cells of patients with Behçet's Disease. Cell Mol Biol (Noisy-le-grand). 2017 Oct 31;63(10):116-121 Authors: Irtegun Kandemir S, Tekin MA, Bozkurt M, Dagli AZ, Kalkanli-Tas S Abstract Behçet's disease (BD) is achronic inflammatory disorder characterized by recurrent oral and genital ulcers, uveitis and skin lesions. Although,the pathogenesis of BD remains poorly understood, excessive or dysregulatedcytokine production including IL-10 is associated with BD. Revealin...
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The association between murine double minute 2 (MDM2) rs2279744 and endometrial cancer risk in a Chinese Han population.
In conclusion, the study found that MDM2 rs2279744 polymorphism was significantly associated with endometrial cancer risk in a Chinese Han population. PMID: 29096752 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Interleukin-6 secreted by oral cancer- associated fibroblast accelerated VEGF expression in tumor and stroma cells.
In this study, we examined the correlation between IL-6 and CAF and the role of this correlation on VEGF production. In this study, quantitative expression level of IL-6 and VEGF in CAF and Oral Cancer Cells (OCCs) examined through Real Time PCR and ELISA and western blot analysis. In addition, maintenance and retention of IL-6 and VEGF checked out in co-culture experiment of CAF and OCC cells. These experiments demonstrated that in oral cancer, CAF cell line secretes significantly more IL-6 than OCC. Also IL-6 is a factor that causes VEGF secretion in CAF cell line. CAF is the basic and the most essential source for produ...
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Overview of ribosomal and non-ribosomal antimicrobial peptides produced by Gram positive bacteria.
Authors: Tajbakhsh M, Karimi A, Fallah F, Akhavan MM Abstract The increasing incidence of antimicrobial resistance bacterial infection and decreasing effectiveness of conventional antibiotics to treatment have caused serious problems worldwide. The demand for new generationantibiotics to combat microbial pathogens is imperative. Cationic antimicrobial peptides (AMPs) with different sources from prokaryotic to complex eukaryotic organisms, with variable length, amino acid composition and secondary structure, have been consideredduring the past decades.  The advantages of large number of AMPs are related to broa...
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Ziziphus Jujube: a review study of its anticancer effects in various tumor models invitro and invivo.
Authors: Ebrahimi S, Mollaei H, Hoshyar R Abstract The growing rate of cancer incidences and inefficiency of current therapies have made scientists to find novel agents such as medicinal plants. Ziziphus jujube is one of these plants whose pharmaceutical effects have been studied for a long time. Recent investigations have proved that bioactive compounds of Ziziphus jujube including triterpenic acids, flavonoids, cerebrosides, phenolic acids, α tocopherol, β carotene, and polysaccharides have anti-proliferative effects on several cancerous cell lines and animals. The present study aimed to cover all prev...
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Genetic barcoding and phylogenetic analysis of dusky cotton bug (Oxycarenus hyalinipennis) using mitochondrial cytochrome c oxidase I gene.
Authors: Khan AZ, Amad I, Shaheen S, Hussain K, Hafeez F, Farooq M, Noor Ul Ayan H Abstract Cotton dusky bug (Oxycarenus spp.) mostly attack on cash crops such as Gossypium, Cola and Hibiscus which affect the national economy therefore sustainable pest management is needed. Cytochrome c oxidase I (COI) gene is utilized as marker gene for DNA barcoding, genetic and ecological study of insects. In present study insect (cotton dusky bug) samples were collected from cotton fields in Faisalabad. COI gene was amplified from genomic DNA of bug and cloned into pTZ57R/T vector (Fermentas). The clone was sent to Macrogen (So...
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Matrix metalloproteinase-1 (MMP-1) rs1799750 polymorphism is associated with nasopharyngeal carcinoma (NPC) risk.
In conclusion, this meta-analysis showed that MMP-1 rs1799750 polymorphism was significantly associated with NPC risk. PMID: 29096757 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Endothelial nitric oxide synthase 27VNTR (4b/4a) gene polymorphism and the risk of diabetic microvascular complications in Chinese populations.
Authors: Zhang X, Yang Z, Chen X Abstract To access the association of endothelial nitric oxide synthase (eNOS) gene 27VNTR (4b/4a) polymorphism with diabetic microvascular complications (DMI) susceptibility in Chinese populations. Data were retrieved in a systematic manner on PubMed database, ISI Web of Knowledge, the Cochrane Library, Chinese National Knowledge Infrastructure, and Wanfang, as well as manual searching of the references of the identified articles. Odds ratio (OR) and 95% confidence interval were used to evaluate the strength of associations. Potential sources of heterogeneity and publication bias w...
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Circulating macrophage migration inhibitory factor levels and its polymorphisms in systemic lupus erythematosus: A meta-analysis.
Authors: Bae SC, Lee YH Abstract The present study aimed to systemically review the evidence regarding the relationship between circulating macrophage migration inhibitory factor (MIF) levels and systemic lupus erythematosus (SLE), as well as the associations between several polymorphisms in the MIF gene and SLE susceptibility. We performed a meta-analysis of serum/plasma levels of MIF in SLE patients and controls and evaluated evidence of associations between the MIF -173 C/G allele and -794CATT5-8 polymorphisms and the associated risk for SLE. Nine studies were included in this meta-analysis. Meta-analysis indica...
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

ALOX5AP rs10507391 polymorphism and the risk of ischemic stroke in Caucasians: an update meta-analysis.
In conclusion, this study found that ALOX5AP rs10507391 polymorphism was associated with ischemic stroke risk in Caucasians. PMID: 29096760 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Bioactive chemical compounds in Eremurus persicus (Joub. & Spach) Boiss. essential oil and their health implications.
In conclusion, our results suggested that this EO possesses a wide range of bioactive properties that could be useful in nutraceutical, functional foods and cosmeceutical formulations. PMID: 28980914 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - October 6, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Functional role of inflammation in the surgical injury induced vascular remodeling of male albino rats.
Authors: Song X, Li L, Wu Y, Zhang L, Li X Abstract Our present study investigates the cellular and molecular inflammatory events in male albino rat arterial injury. Male albino rats were subjected to longitudinal incision and carotid artery clamping for the duration of 45 days. Heat shock protein (HSP) 27, HSP70, HSP47 and Nuclear Factor kappa B (NF-κB) expressions were determined by qPCR and Western blot method. The morphology of vessel wall alteration was studied by the light microscopy. The expression of NF-κB was found to be increased after ten days of carotid artery injury. The qPCR and Western bl...
Source: Cellular and Molecular Biology - October 6, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Cetuximab has an inhibitory effect on cell motility in SCC-4 oral squamous cell carcinoma cell line.
This study aimed to evaluate the effect of treatment with Cetuximab on cell migration and invasion in OSCC cells, by using the SCC-4 cell line. Cell migration and cell invasion assay were performed and actin cytoskeleton of control and treated with Cetuximab cells were evaluated. Differences were considered significant when p<0.05.Cetuximab inhibited the migration of SCC-4 cells at three concentrations: 1 µg/mL, 50 µg/mL and 100 µg/mL (p<0.0001) in a dose-dependent manner. The number of SCC-4 treated cells with 1 μg/mL that migrated through the membrane was statistically different from 50...
Source: Cellular and Molecular Biology - October 6, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

FAM196B acts as oncogene and promotes proliferation of gastric cancer cells through AKT signaling pathway.
Authors: Zhang J, Tong DD, Xue M, Jiang QY, Wang XF, Yang PB, Ni L, Zhao LY, Huang C Abstract Gastric cancer (GC) is the second leading cause of cancer-related deaths worldwide, but the mechanisms remain unknown. Here we report that family with sequence similarity 196 member B (FAM196B) is highly expressed in primary GC tissues and the expression level is correlated with the clinicopathologic characteristics of GC. In this experiment, knockdown of FAM196B suppressed GC cell proliferation and induced G1/G0 to S phase cell cycle arrest by regulating Cyclin D1, Cyclin A and CDK2 expressions. Furthermore, we investigat...
Source: Cellular and Molecular Biology - October 6, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Some heavy metals in Luciobarbus esocinus for public consumption and consumer protection.
In this study, the concentrations of some heavy metals were determined in the muscle of Luciobarbus esocinus Heckel, 1843 and in water where they live. The results were evaluated in term of potential human risk of consumption of this fish and the relationship between the heavy metal load of fish and some of their biological aspects (weight, length and sex). In addition, the accumulation factor of heavy metals in the muscle of L. esocinus were also determined. The Cr, Pb and Ni were not found in detectable levels according to results obtained by ICP. Only Cu, Zn and Fe were detected. It was found that heavy metals concentra...
Source: Cellular and Molecular Biology - October 6, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Risk assessment of a formamidine pesticide, Amitraz, focusing on thyroid hormone receptors (TRs) in rainbow trout, Oncorhynchus mykiss.
Authors: Meriç Turgut I, Keskin E Abstract Amitraz, a formamidine pesticide, and their metabolites have the potential to disrupt endocrine homeostasis in a variety of organisms, nevertheless there is a lack of information concerning such effects and underlying mechanisms in any fish species.To evaluate the potential impacts of Trasil (EC; active constituent 200 g amitraz/L), a commercial product of amitraz, on thyroid hormone (TH) homeostasis of rainbow trout (Oncorhynchus mykiss); mRNA levels of thyroid hormone receptors (TRs), TRα and TRβ, were determined by RT-PCR soon after sub-lethal administ...
Source: Cellular and Molecular Biology - October 6, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Transcriptomic analysis of human non-small lung cancer cells A549 treated by one synthetic curcumin derivative MHMD.
Authors: Zhou G, Sun G, Zhou Y, Wang Q Abstract In our previous studies, we have identified one curcumin analog MHMD could induce apoptosis of lung cancer cells A549 via extrinsic and intrinsic pathways in our previous studies. But the specific regulatory genes and molecular mechanisms remain poorly understood. Here, the transcriptomic profile of A549 cells was detected with RNA-seq technique after MHMD treatment at 48 h. A total of 16584651 clean data from 21831774 sequence reads were obtained and 80.75% of them could be mapped on the human test genome. 18635 unigenes with the mean length of 4027 bp were finally a...
Source: Cellular and Molecular Biology - October 6, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Protease sensitivity and redistribution of CD71 and glycophorin A on K562 cells.
Authors: Yu CH, Wang H, Wang Y, Cui NX, Zhao X, Rong L, Yi ZC Abstract Transmembrane proteins are delivered to plasma membrane from the endoplasmic reticulum and Golgi complex by vesicular transport along with the cytoskeletal network. Disruption of this process likely affects transmembrane protein expression. K562 cells were digested with Streptomyces griseus protease for different periods of time, and then re-cultured with different cytoskeletal and glycosylation inhibitors. Cell viability and surface expression of transferrin receptor (CD71) and glycophorin A (GPA) were analyzed before and after re-culture by fl...
Source: Cellular and Molecular Biology - October 6, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

rs3918242 variant genotype frequency and increased TIMP-2 and MMP-9 expression are positively correlated with cancer invasion in urinary bladder cancer.
Authors: Pençe S, Özbek E, Ozan Tiryakioğlu N, Ersoy Tunali N, Pençe HH, Tunali H Abstract To study the role of MMP9 and TIMP2 genotypes and expression in predisposition to bladder cancer and relation with metastasis. 100 urinary bladder cancer patients and 100 healthy controls were included in the study. rs3918242 and rs8179090 genotypes were determined with PCR-RFLP. Quantitative real-time polymerase chain reaction was employed to assess the MMP-9 and TIMP-2 expression in tumors and adjacent healthy tissues. Variant genotype (TT) for rs3918242 polymorphism and rs8179090 variant genotype a...
Source: Cellular and Molecular Biology - October 6, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Morphological, genetic and pigment diversity of Nerium indicum Mill in Iran.
Authors: Mohammadi F, Sharifi-Sirchi G, Samsampour D Abstract The Nerium indicum Mill organs and parts of the 40 genotypes were sampled from 5 habitats of southand south east of Iran. In total, 15 morphological and pigmentvariables were measured. Analysis of variance was carried out based on completely randomized design and revealed significance differencesat p ≤ 0.05, 0.01 and 0.001 for the most variablesindicating a large-scale diversity among the genotypes. Cluster analysis divided the genotypes into 3 main groups. The first and second principal components had 34.73% and 18.67% of the variance, respectively. ...
Source: Cellular and Molecular Biology - October 6, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

STAT3 regulates cytokine expression in peripheral blood mononuclear cells from asthma patients.
Authors: Yang Q, Xu W, Long Y, Kuang J, Li J Abstract Asthma is a common long term inflammatory disease of the airways. This disease affected millions of people worldwide. Recently, it is demonstrated that signal transducer and activator of transcription 3 (STAT3) plays critical role in asthma occurrence. In the current study, we isolated peripheral blood mononuclear cells (PBMCs) from patients with mild and moderate asthma, and then determined the correlation between STAT3 and cytokine expression. We found that the concentration and mRNA level of cytokines was increased in PBMCs from asthma patients. The concentra...
Source: Cellular and Molecular Biology - October 6, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

miR-214 modulates cisplatin sensitivity of osteosarcoma cells through regulation of anaerobic glycolysis.
In this study, we report miR-214 contributes to cisplatin resistance in osteosarcoma cells. Overexpression of miR-214 decreased the cisplatin sensitivity. By establishing an osteosarcoma cisplatin resistant cell line, we find miR-214 is significantly upregulated in cisplatin resistant cells. Moreover, we show miR-214 promotes anaerobic glycolysis rates of osteosarcoma cells but suppresses mitochondrial oxidative phosphorylation. Consistently, cisplatin resistant cells exhibit upregulated glycolysis but decreased mitochondrial oxidative phosphorylation, a phenotype called "Warburg effect". Finally, we demonstrate ...
Source: Cellular and Molecular Biology - October 6, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Insufficient decontamination in Sewage treatment plants induce the risk of artificial selection of extended-spectrum β-lactamase producing Escherichia coli.
Insufficient decontamination in Sewage treatment plants induce the risk of artificial selection of extended-spectrum β-lactamase producing Escherichia coli. Cell Mol Biol (Noisy-le-grand). 2017 Sep 30;63(9):80-86 Authors: Gundogdu A, Kilic H, Kurekci C, Alp E Abstract Quantitative data about extended-spectrum beta-lactamase producing E. coli (ESBLEC) in the wastewaters are scarce, especially in developing countries. These data could be useful to raise awareness about the potential risk of spreading ESBLEC strains in the community. Water samples were collected weekly over a 10-week period, from one...
Source: Cellular and Molecular Biology - October 6, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Antioxidant activities, anticancer activity and polyphenolics profile, of leaf, fruit and stem extracts of Pistacia lentiscus from Tunisia.
Authors: Yemmen M, Landolsi A, Ben Hamida J, Mégraud F, Trabelsi Ayadi M Abstract Plant derived compounds have played an important role in the development of several clinically useful anticancer drugs. The aim of the present study was 1) to evaluate for the first time the anti-proliferative activity of a polyphenol enriched extract obtained from leaf, fruit and stem of Tunisian variety of Pistacia lentiscus against two cultured cancer cells, and 2) to carry out a phytochemical analysis of vegetable extracts particularly by determining the chemical composition of phenolics (total polyphenols, flavonoids and c...
Source: Cellular and Molecular Biology - October 6, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research