MicroRNA-223 is involved in the pathogenesis of atopic dermatitis by affecting histamine-N-methyltransferase.
In this study, we identified upregulated miR-223 in the whole blood cells of a large group of AD patients. What's more, we found for the first time that one of the major histamine degradation enzymes, histamine-N-methyltransferase (HNMT), was increased in AD patients and AD model mice. Although there was one miR-223 binding site in the 3'- untranslated region of the HNMT gene, HNMT were not inhibited by miR-223. Taken together, it suggested that miR-223 participates in AD through upregulating HNMT indirectly to degrade the excessive histamine. PMID: 29506638 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - March 8, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Genetic polymorphisms in human telomerase reverse transcriptase (hTERT) gene polymorphisms do not associated with breast cancer in patients in a turkish population: hospital-based case-control study.
In this study, it was found that there was no statistical difference between hTERT rs2736109 G>A, rs2735940 T>C, rs2853669 A>G, rs2736098 G>A, and rs2736100 T>G polymorphisms that can be associated with risk of BC. PMID: 29506639 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - March 8, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

A rare case of complicated pericardial effusion with Elizabethkingia meningoseptica from Iran.
We report a rare case of complicated pericardial effusion due to E. meningoseptica in a 2-year-old boy, who was admitted with chief complaints of fever and tachypnea (mentioned by his parents) and suffered from a rare lung malignancy (lymphangioleiomyomatosis). He was successfully treated with vancomycin.  E. meningoseptica infection is a rare situation in immunocompetent hosts, and we concluded that this infection was probably originated from device medicine or even hands of healthcare workers. PMID: 29506640 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - March 8, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effects of various glutamine concentrations on gene expression and steviol glycosides accumulation in Stevia rebaudiana Bertoni.
Authors: Esmaeili F, Ghaheri M, Kahrizi D, Mansouri M, Safavi SM, Ghorbani T, Muhammadi S, Rahmanian E, Vaziri S Abstract Stevia rebaudiana Bertoni is one of the most important biologically sourced and low-calorie sweeteners that contains a lots of Steviol glycosides. Tissue culture is the best method for propagation of stevia and micro nutrients can affect both morphological traits and steviol glycosides production. In the present study, we investigated the effect of different concentrations of glutamine (10, 20, 30 and 40 g/l) on expression of UGT74G1 and UGT76G1 genes and stevioside and rebaudioside A accumulati...
Source: Cellular and Molecular Biology - February 15, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effect of Stevia rebaudiana Bertoni extract on sexual dysfunction in Streptozotocin-induced diabetic male rats.
Authors: Ghaheri M, Miraghaee S, Babaei A, Mohammadi B, Kahrizi D, Saivosh Haghighi ZM, Bahrami G Abstract Stevia rebaudiana Bertoni has been used locally as a non-calorie sweetener in medicine and diabetic diet which claimed to have aphrodisiac properties, although no scientific data of this function have been reported. The aim of this study was to investigate the effect of S. rebaudiana extract on sexual dysfunction, testosterone levels and number of Leydig cells in Streptozotocin (STZ)-induced diabetic male rats. A total of 28 diabetic male rats were randomly divided into 4 groups: diabetic group without any ext...
Source: Cellular and Molecular Biology - February 15, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effect of nitrogen sources on gene expression of Stevia rebaudiana (Bertoni) under in vitro conditions.
In this study the effects of nitrogen influenced by different concentrations of NH4NO3 (0, 825 and 1650 mg/l) and KNO3 (0, 950 and 1900 mg/l) is examined in MS medium. To analysis the UGT74G1 and UGT76G1 genes expression, involved in the synthesis of SGs, RT-qPCR technique was performed. Data showed that there were significant differences between all media. The shoot length, seedlings dry weight and leaf fresh weight of stevia increased with applying NH4NO3 along with KNO3. The highest expression of UGT74G1 gene, was observed in plantlets grown on MS medium with 0 mg/l NH4NO3 and 950 mg/l KNO3 (1.291 total lab unit) but th...
Source: Cellular and Molecular Biology - February 15, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effects of life cycle and leaves location on gene expression and glycoside biosynthesis pathway in Stevia rebaudiana Bertoni.
Authors: Ghaheri M, Adibrad E, Safavi SM, Kahrizi D, Soroush A, Muhammadi S, Ghorbani T, Sabzevari A, Ansarypour Z, Rahmanian E Abstract Stevia rebaudiana Bertoni is One of the most important biologically sourced and low-calorie sweeteners that known as "Sweet Weed". It contains steviol glycosides that they are about 200-300 times sweeter than sucrose. Tissue culture is the best method with high efficiency that can overcome to problems of traditional methods, and it is the most useful tools for studying stress tolerance mechanisms under in vitro conditions to obtain drought tolerance. In the present resea...
Source: Cellular and Molecular Biology - February 15, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Investigation of different concentrations of MS media effects on gene expression and steviol glycosides accumulation in Stevia rebaudiana Bertoni.
Authors: Kahrizi D, Ghaheri M, Yari Z, Yari K, Bahraminejad S Abstract Stevia rebaudiana Bertoni is one of two species that contains steviol glycosides. Among steviol glycosides that extracted from leaves, stevioside and rebaudioside A are the two major and the sweetest glycosides that are about 200-300 times sweeter than sucrose with zero calories. The best method for stevia propagation is tissue culture. So, for investigation of nutrients in medium, we studied the effect of different concentrations of MS media (MS, 0.5 MS, 0.25 MS, 0 MS) on morphological traits, UGT74G1 and UGT76G1 genes expression and accumulati...
Source: Cellular and Molecular Biology - February 15, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effects of different concentrations of mannitol on gene expression in Stevia rebaudiana Bertoni.
Authors: Hashempoor S, Ghaheri M, Kahrizi D, Kazemi N, Muhammadi S, Safavi SM, Ghorbani T, Rahmanian E, Heshmatpanaah M Abstract Stevia rebaudiana Bertoni is one of the most important herbal sweetener plants from Asteracea family that have a lot of Steviol glycosides. Among different methods, tissue culture is the best way with high efficiency that is useful for studying stress tolerance mechanisms to obtain drought tolerance of stevia. For this purpose, different concentrations of mannitol (0, 10, 20, 30, 40, 50 mg/l) were used as various treatments in the culture medium of stevia. According to the results, the hi...
Source: Cellular and Molecular Biology - February 15, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Metal oxides as a biostimulator for upregulation of genes involved in the biosynthesis of Rebaudioside- A.
Authors: Mirzaei A, Kavoosi G, Dezhsetan S, Behnamian M Abstract Stevia rebaudiana Bertoni is a kind of perennial medicinal plant with sweetening properties which belongs to Asteraceae family. Its leaves with fundamental glycoside compounds consist of both a sugar part and a non-sugar sector. One of the glycoside compounds is Rebaudioside- A which has a greater importance in business. This experiment was conducted to evaluate the effects of Ag2O, CrO3, PbO, Fe2O3, BaO and TiO2 on the expression pattern of these genes in the Stevia rebaudiana. Rebaudioside- A biosynthesis was repeated 3 times with concentrations of ...
Source: Cellular and Molecular Biology - February 15, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The Effects of different nitrogen doses on antioxidant and antimicrobial activity of Stevia (Stevia rebaudiana Bert.).
In this study, the efficiency of the different nitrogen doses (0, 5, 10, 15 and 20 kg ha-1) on biological activity levels (antioxidant and antimicrobial activity) of Stevia rebaudiana Bert. was investigated. In addition, methanol extracts were obtained by maceration method from different doses of fertilizer applied stevia. The components in methanol extracts of plants were determined by gas chromatography-mass spectrometry (GC-MS) method. Antimicrobial activities of stevia extracts were investigated by microdilution method. The antioxidant activity evaluated by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical s...
Source: Cellular and Molecular Biology - February 15, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Callus induction and somatic embryogenesis in Stevia rebaudiana Bertoni as a medicinal plant.
Authors: Keshvari T, Najaphy A, Kahrizi D, Zebarjadi A Abstract Stevia rebaudiana (Bert.) from Asteraceae family is a useful medicinal plant that prevents and cures diabetes, blood pressure, weight gain and tooth decay. Due to self-incompatibility in stevia, somatic embryo investigation for artificial seed production is valuable in this plant. In order to evaluate the callus induction characteristics in stevia, a factorial experiment was laid out based on a completely randomized design with three replications. The factors included ten hormone combinations and control, two kinds of media (MS and B5) and two types of...
Source: Cellular and Molecular Biology - February 15, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effect of decapitation and exogenous application of gibberellic acid (GA3) and cytokinin (CK) on some physiological characteristics of stevia.
This study was designed out to investigate whether decapitation and exogenous application of plant growth regulators enhance the productivity of stevia through breaking the apical dominance and increasing physiological characteristics. Experiment was carried out as a factorial in randomized complete block design with three replications. Factors were consisted two agricultural practices (Decapitation and No-decapitation) and eight foliar spray including without spray as control, water spray, GA3 (300, 600 and 900 µm) and CK (100, 200 and 400 µm). The results of the present investigation indicated a positive resp...
Source: Cellular and Molecular Biology - February 15, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

L-Carnitine supplementation increases expression of PPAR- γ and glucose transporters in skeletal muscle of chronically and acutely exercised rats.
In this study, the effects of L-Carnitine supplementation on the lipid peroxidation and expression of PPAR-γ and glucose transporters in the liver and muscles of chronically and acutely exercised rats were investigated. A total of 42 male Wistar Albino rats (8-week-old) were divided into six groups as follows: Control, L-Carnitine, Chronic Exercise (CE), Chronic Exercise + L-Carnitine, Acute Exercise (AE) and L-Carnitine + Acute Exercise. Chronic exercise consists of 30 m/min, 30 min/day, and 5 days/week for 6 weeks. Rats in the acute exercise groups were run on the treadmill at 30 m/min until exhaustion. L-Carnitine...
Source: Cellular and Molecular Biology - February 9, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Long term treatment by mesenchymal stem cells conditioned medium modulates cellular, molecular and behavioral aspects of adjuvant-induced arthritis.
Authors: Nazemian V, Manaheji H, Sharifi AM, Zaringhalam J Abstract Neuroinflammation plays a crucial role in expression of symptoms of numerous autoimmune and neurodegenerative diseases such as pain during rheumatoid arthritis. Overproduction of pro-inflammatory cytokines and activation of intracellular signaling pathways have been strongly implicated in the generation of pathological pain states, particularly at central nervous system sites and induction of spinal neuroinflammatory symptoms. The wide ranges of research to define new therapeutic approaches, including neuroimmune-modulators like stem cells are in p...
Source: Cellular and Molecular Biology - February 9, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Knockdown of MPP8 suppresses cell proliferation via regulation of HOXA5 in non-small cell lung cancer cells.
Authors: Gao XY, Qiao YL, Zhang Y, Wang J, Shen X, Xu CW Abstract M-phase phosphoprotein 8 (MPP8) is reported to be closely implicated in cancer initiation and progression. In addition, the homeobox gene HOXA5 has been shown to play critical roles in hematopoiesis, embryogenesis, and tumorigenesis. Nevertheless, the functional relevance of MPP8 and it's relation with HOXA5 in non-small cell lung cancer (NSCLC) is unknown. Therefore, the present study aimed to detect the expression profile of MPP8 in NSCLC and further explore it's biological roles in lung cancer cells. Cell proliferation was measured by CCK-8 assay ...
Source: Cellular and Molecular Biology - February 9, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The relationship between the variations in G γ and Aγ promotors and the Hereditary Persistence of Fetal Hemoglobin (HPFH).
The relationship between the variations in Gγ and Aγ promotors and the Hereditary Persistence of Fetal Hemoglobin (HPFH). Cell Mol Biol (Noisy-le-grand). 2018 Jan 31;64(1):32-39 Authors: Aydin M, Rencuzogullari E, Dalyan A, Bayram S, Genc A Abstract In the present study, sixty-two samples that have 1.5% and upper level of fetal hemoglobin (HbF), were examined to investigate the relationship between HbF level and non-deletional mutations in both Gγ (G gamma) globin (HBG2) and Aγ (A gamma) globin (HBG1) genes. Four variations were observed in the promotor of Gγ gene, which a...
Source: Cellular and Molecular Biology - February 9, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The effects of caveolin1 on β cell proliferation.
The effects of caveolin1 on β cell proliferation. Cell Mol Biol (Noisy-le-grand). 2018 Jan 31;64(1):40-46 Authors: Li H, Peng H, Xu H, Xu F, Lin S, Lin K, Zeng W, Zeng L Abstract Our study aims to access the influence of caveolin1 (CAV1) on β cell expression profiles. We knocked down the expression of CAV1 in both NIT-1 cells and islets isolated from C57BL/6J mice using an RNA interference technique, which was realized by the transfer of an shRNA vector targeting CAV1 mRNA into NIT-1 cells or islets through latent virus infection. First, we identified the change in gene expression profiles in...
Source: Cellular and Molecular Biology - February 9, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effects of different levels of dietary Citrus Limon essential oil on some blood parameters and antioxidant status in Afshari Ewes.
This study was conducted to investigate the effects of different levels of CLO on blood parameters and antioxidant status in Afshari ewes. Six adults Afshari ewes (3-4 years old and 51±5 kg) were randomly allocated to 3×3 Latin square design with three diets in 21 days period. Dietary treatments included:1) control diet, 2) control diet with 200 mg/day CLO, and 3) control diet with 400 mg/day CLO. To evaluate the antioxidant effect of the CLO, sustainable elimination of free radicals by DPPH and ABTS methods were used. The antioxidant activity of essential oils in DPPH method at doses of 32.5, 45, 130, 260 and...
Source: Cellular and Molecular Biology - February 9, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

A comparative analysis of RAPD and ISSR markers for assessing genetic diversity in Iranian populations of Nigella sativa L.
Authors: Hosseini KorehKhosravi S, Masoumiasl A, Dehdari M Abstract The genetic diversity of 18 Iranian populations of Nigella sativa L. was tested using RAPD (10 primers) and ISSR (12 primers) molecular markers. RAPD primers produced 103 acceptable bands and out of them, 42 bands were polymorphic and the total percentage of polymorphism was estimated 40.5%. Out of the 12 ISSR primers, only 5 of them produced acceptable bands. Five ISSR primers generated 39 amplified products and out of them, 19 bands were polymorphic and the total percentage of polymorphism was estimated 57.4%. As a result, the level of polymorphi...
Source: Cellular and Molecular Biology - February 9, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

A new target for the treatment of endometrium cancer by succinic acid.
Authors: Iplik ES, Catmakas T, Cakmakoglu B Abstract Endometrium cancer is the most common invasive gynecologic malignancy in developed countries. Succinic acid (CO2HCH2-CH2CO2H) is a type of dibasic acid that has uncolored crystal. Succinic acid is used in bakery products and aromatized products. It is naturally found in some vegetables. Succinic acid has no adverse effects because it is metabolized by body cells and has a role in the tricarboxylic acid cycle (TCA) as a cycle media component. The TCA cycle and its enzyme components have some crucial roles for basal cell metabolism. Any mistakes, concentration diff...
Source: Cellular and Molecular Biology - February 9, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Construction and recombinant expression of Pseudomonas aeruginosa truncated exotoxin A in Escherichia coli.
Authors: Qaiser H, Aslam F, Iftikhar S, Farooq A Abstract Pseudomonas aeruginosa exotoxin A (PE) is a bacterial toxin composed of three domains namely: cell binding, translocation and enzymatic domain. The cytotoxic activity of PE is attributed to the enzymatic domain, which inhibits protein synthesis through ADP-ribosylation of EF-2. PE can be genetically modified to fight cancer. In this regard, a truncated and modified form of PE was produced that could be used for more potent immunotoxins. This modified form termed PE38KDEL was completely devoid of cell binding domain and parts of translocation domain II and Ib...
Source: Cellular and Molecular Biology - February 9, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Frequency Determination of Carbapenem-Resistant Klebsiella Pneumoniae (CRKP) Isolated from hospitals in Isfahan of Iran and Evaluation of Synergistic Effect of Colistin and Meropenem on them.
This study was designed to identification of carbapenemase producing isolates of K. pneumoniae via phenotypic and genotypic methods as well as evaluation of colistin-meropenem combination therapy potential. This study was carried out in Isfahan, of Iran on 100 samples from Alzahra and Khorshid hospitals in 2017. The Modified Hodge Test (MHT) was used to investigate the carbapenemase presence. The minimum inhibitory concentration (MIC) and the Fractional Inhibitory Concentration (FIC) were determined using broth macrodilution and checkerboard assays (respectively) for both meropenem and colistin. The bla-KPC gene was studie...
Source: Cellular and Molecular Biology - February 9, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Identification of phenolic compounds, antioxidant activity and anti-cancer effects of the extract obtained from the shoots of Ornithogalum narbonense  L.
This study aimed to examine the anti-cancer and antioxidant properties and identify the phenolic content of methanol extract obtained from the shoots of the Ornithogalum narbonense L. (OR) plant, which is used for folk-medicine and food in the Sanliurfa region of Turkey. The antioxidant activity of the extract was investigated using total phenolics, flavonoids, ABTS and CUPRAC methods. Phenolic component analysis of the plant extract was performed by LC-MS/MS. The anti-cancer property of OR extract was investigated on human colon (DLD-1), endometrium (ECC-1) cancer cells and embryonic kidney (HEK-293) cells. Cytotoxic effe...
Source: Cellular and Molecular Biology - February 9, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Correlation between serum leptin and its gene expression to the anthropometric measures in overweight and obese children.
Authors: Eldosouky MK, Abdu Allah AM, AbdElmoneim A, Al-Ahmadi NS Abstract Obesity is a multifactor disorder with evidence supporting the role of the genetic factor in its etiology. The present study evaluates the relationship between leptin G2548A (rs7799039) and leptin receptors (Gln223Arg (rs1137101) genotyping and its leptin level and the risk of childhood obesity. This case-control study was conducted on 168 overweight and obese Saudi children and 126 non obese one served as control. Fasting insulin, leptin, blood glucose, lipid profile levels were measured. HOMA- IR, and BMI were assessed. Genotyping of lepti...
Source: Cellular and Molecular Biology - February 9, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Organ transplantation and donation from the point of view of medical students in Iran: Ethical aspects and knowledge.
Authors: Abbasi M, Kiani M, Ahmadi M, Salehi B Abstract Organ transplantation is an effective process that prolongs the lives of individuals suffering from incapacitating conditions. The aim of this study was to evaluate the view point of medical students in Iran regarding ethical aspects and knowledge on organ transplantation and donation. The participants included 165 medical students from different faculties of Shahid Beheshti University of Medical Sciences, Tehran, Iran. They were assessed using a reliable questionnaire that examined their ethical aspects and knowledge regarding organ transplantation and donati...
Source: Cellular and Molecular Biology - February 9, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Differentially expressed Secretoglobin 1C1 gene in nasal polyposis.
In this study, to determine the relationship between the Secretoglobin 1C1 gene promotor polymorphisms and the gene expression, the levels of 48 subjects were sequenced (24 patients with NP and 22 controls without sinonasal disease). The levels' expression of Secretoglobin 1C1 in the subjects' nasal mucosa was also detected using RT-PCR.  In this study, the level of Secretoglobin 1C1's expression increased in NP (P= 0.003). Three polymorphisms were detected in the Secretoglobin 1C1 gene's promotor. The rs113795008 and rs2280540 variations were significantly high in NP (P= 0.005, P= 0.045). The the rs113795008 homozygo...
Source: Cellular and Molecular Biology - February 9, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

High-fat diet stimulates the gut pathogenic microbiota and maintains hepatic injury in antibiotic-treated rats.
Authors: Al-Daihan S, Ben Bacha A, Al-Dbass AM, Alonazi MA, Bhat RS Abstract The gut and the liver are closely linked to each other, as changes in the gut microbiota can play a significant role in the development of many liver diseases. Gut bacteria respond rapidly to changes in diet and thus can affect the liver through their metabolites. The impact of a high lipid diet on the liver in the presence of an altered gut flora modulated by ampicillin was investigated. The study was performed on 30 male Western albino rats randomly divided into 3 groups: control (phosphate buffered saline treated), group II (ampicillin ...
Source: Cellular and Molecular Biology - February 9, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

High temperature requirement factor A1 (HTRA1) regulates the activation of latent TGF- β1 in keloid fibroblasts.
High temperature requirement factor A1 (HTRA1) regulates the activation of latent TGF-β1 in keloid fibroblasts. Cell Mol Biol (Noisy-le-grand). 2018 Jan 31;64(1):107-110 Authors: Jin SF, Wang YX, Xu N, Sun Q, Wang CC, Lv MZ, Sun X, Guo S Abstract Scar treatments are considered a major issue in the plastic surgery field. Activation of the transforming growth factor-β (TGF-β)-mediated signaling pathway plays a key role in the scar pathogeneses, and high temperature requirement factor A1 (HTRA1) inhibits TGF-β1 activation in tumor cells. Our study aims to investigate the role of HTRA1 ...
Source: Cellular and Molecular Biology - February 9, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Testosterone to estrogen conversion is not responsible for the vasodilating effects of testosterone ex vivo.
Authors: Tan S, Yi D, Zhu W, Sun R, Wei M Abstract Men have a higher incidence of cardiovascular disease, but poorer vasodilatation than women. However, testosterone exerts vasodilating actions ex vivo. We aimed to determine if reactive oxygen species (ROS) produced in vivo could cause 'eNOS uncoupling' that accounts for the disparity between in vivo and ex vivo results. Ovariectomized SHR and WKY rats were divided into 3 groups: untreated, estradiol benzoate (EB) treated, and testosterone propionate (TP) treated. EB and TP rats were treated for 8 weeks, and blood pressure, serum estrogen, progesterone, and testost...
Source: Cellular and Molecular Biology - February 9, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Molecular mechanisms underlying the participation of Ribonuclease T2 gene into self-incompatibility of Citrus grandis var. Shatianyu Hort.
Authors: Qin XM, Zhang Y, Liu YJ, Guo DN, Li HM Abstract The RIB (ribonuclease T2) of Citrus grandis var. Shatianyu Hort involved in self-incompatibility (SI) mechanism was identified by prokaryotic expression. RT-qPCR results showed that the expression level of RIB in self pollinated stigma is significantly higher than that in cross pollinated stigma. A vector for prokaryotic expression of RIB was constructed after codon-optimization, and the recombinant protein was induced and purified. In vitro pollen germination test indicated that the RIB protein markedly inhibited pollen germination and pollen tube growth. Th...
Source: Cellular and Molecular Biology - February 9, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Ethanol promotes apoptosis in rat ovarian granulosa cells via the Bcl-2 family dependent intrinsic apoptotic pathway.
This study recruited rat ovarian granulosa cells, the primary effector of ovary, to investigate the effects of ethanol on cell apoptosis and explore potential mechanism. Ovarian granulosa cells treated with ethanol for three hours manifested observable reduction in cell viability and apparent apoptosis. In the presence of 200 and 300 mmol/l of ethanol, the percentages of apoptotic cells increased to 33% and 36%, respectively. In addition, apoptosis related caspase-3 activity was elevated with increasing concentrations of ethanol, suggesting a dose dependent effect. Furthermore, high concentrations of ethanol significantly ...
Source: Cellular and Molecular Biology - February 9, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Association between Vitamin D level and/or deficiency, and systemic lupus erythematosus: a meta-analysis.
This study aimed to evaluate the relationship between the level of 25-hydroxyvitamin D [25(OH)D] and systemic lupus erythematosus (SLE). We searched the PUBMED, EMBASE, and Cochrane databases and performed a meta-analysis examining the vitamin D level and/or deficiency in patients with SLE, compared with that in healthy controls. In total, 18 studies consisting of 1,083 patients with SLE and 1,273 controls were included. Vitamin D level was significantly lower in the SLE group than in the control group (standardized mean difference [SMD] = -0.938, 95% CI = -1.352 to -0.524, p = 9.0 × 10-6). Stratification by ethnicit...
Source: Cellular and Molecular Biology - February 9, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Circulating prolactin levels and Behcet's disease: A meta-analysis.
This study aimed to summarize the current evidence on the relationship between circulating prolactin levels and Behcet's disease (BD). We performed a meta-analysis comparing the serum/plasma prolactin levels in patients with BD with those of controls and performed a subgroup analysis based on ethnicity, disease activity, and sex. Ten articles with a total of 320 patients with BD and 259 controls were included. The prolactin levels were not significantly higher in the BD group than in the control group (SMD=0.208, 95% CI=-0.012-0.428, p=0.064). Stratification by ethnicity indicated no elevation in prolactin level in Turkish...
Source: Cellular and Molecular Biology - February 9, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Retraction: Genetic characterization and assessment of demographic bottleneck in Caspian horse population.
Authors: Cellular and Molecular Biology Staff Abstract [This delete the article DOI: 10.14715/cmb/2017.63.11.16.]. PMID: 29334355 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - January 17, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Biomarkers in marine ecosystems monitoring.
Authors: Oliva S PMID: 29307334 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - January 10, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

MIRU-VNTR analysis of Mycobacterium tuberculosis from Tehran, Sistan-Baluchestan, Kermanshah and Hormozgan during 2014 and 2015.
Authors: Baghbanian M, Zandi H, Zamani S, Javadpour S, Hamzehloo GR, Feizabadi MM Abstract Standard 15-locus Mycobacterial Interspersed Repetitive Unit Variable Number Tandem Repeat (MIRU-VNTR) typing of Mycobacterium tuberculosis (MTB) is a valuable instrumentation for TB control. Our knowledge about the genetic diversity of MTB and population structure of MTB circulating in Iran is limited. During 2014-2015, 98 MTB isolates were collected from the TB centers of four provinces of Iran. Isolates were genetically characterized using 15-locus based MIRU-VNTR typing. Ninety-five distinct mycobacterial interspersed rep...
Source: Cellular and Molecular Biology - January 10, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Mutation spectrum of β-globin gene in thalassemia patients at Hasan Sadikin Hospital - West Java Indonesia.
Mutation spectrum of β-globin gene in thalassemia patients at Hasan Sadikin Hospital - West Java Indonesia. Cell Mol Biol (Noisy-le-grand). 2017 Dec 30;63(12):22-24 Authors: Maskoen AM, Rahayu NS, Reniarti L, Susanah S, Laksono B, Fauziah PN, Zada A, Hidayat DS Abstract Thalassemia is the most common hereditary haemolytic anemia in Southeast Asia, in which Indonesia is among countries that are at a high risk for thalassemia. It has been reported that mutation in the beta-globin gene is responsible in severe Thalassemia. However, the spectrum of beta-globin gene mutations in Indonesian population v...
Source: Cellular and Molecular Biology - January 10, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

In vitro effects of novel type M-V-O derivatives on Xanthine Oxidase.
Authors: Kaya MO, Gül GÇK, Kurtuluş F Abstract Xanthine Oxidase (XO) is related with different diseases such as vascular, gout, nephropathy and renal stone diseases that are relevant to high uric acid levels and oxidative stress. Some common natural inhibitors of xanthine oxidase are known as rosmarinic acid and apigenin. With this study, we aimed to determine inhibitory effects of originally synthesized new generation transition metal vanadates on Xanthine Oxidase (XO) from bovine milk. Because, Xanthine oxidase inhibitors are typically used in the treatment of gout and nephropathy and renal stone dis...
Source: Cellular and Molecular Biology - January 10, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Expression of miR-373 and its predicted target genes E-cadherin and CD44 in patients with laryngeal squamous cell carcinoma.
Authors: Timirci-Kahraman Ö, Verim A, Farooqi AA, Turan S, Özkan-Küçük NE, Yaylim İ Abstract Laryngeal squamous cell carcinoma (LSCC) is a genomically complex disease that is difficult to target, and efforts have been made to identify new treatment strategies and   molecular markers that might stratify patients and individualize options for treatment. miR-373 has diametrically opposed roles in different stages and types of cancers. miR-373 has been suggested to quantitatively control E-cadherin and CD44 expression. We studied the expression of miR-373, E-cadherin and CD44 in la...
Source: Cellular and Molecular Biology - January 10, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Protective effect of gel form of gastric gavage applicated aloe vera on ischemia reperfusion injury in renal and lung tissue.
This study aimed to research the effect of aloe vera to prevent renal and lung tissue damage in an experimental ischemia-reperfusion (I/R) injury model. The study included 21 male Wistar Albino rats, which were categorized into control group, n = 7 (no procedures), Sham group n = 7 (I/R); and aloe vera therapy group, n = 7 (aloe vera and I/R). Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and malondialdehyde (MDA) were evaluated from lung and kidney tissues for biochemical investigations. As histopathological, hematoxylin and eosin and anti-iNOS were also examined. In biochemical investigations,...
Source: Cellular and Molecular Biology - January 10, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The effects of apomorphine on paracetamol-induced hepatotoxicity in rats.
Authors: Sehitoglu MH, Yayla M, Kiraz A, Oztopuz RO, Bayir Y, Karaca T, Khalid S, Akpinar E Abstract It is becoming progressively more understandable that overdose of paracetamol in both humans and animals causes severe hepatotoxicity. Apomorphine is known as a neuroprotective agent. Due to the protective effect,  apomorphine had been tested in experimental studies on different models. Findings obtained through series of expriments suggested that apomorphine may also be useful in liver toxicity. The aim of this study is to investigate the relationship among the hepatoprotective mechanism of apomorphine and to ...
Source: Cellular and Molecular Biology - January 10, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Evaluation of STAT3 rs1053004 single nucleotide polymorphism in patients with chronic hepatitis B and hepatocellular carcinoma.
Authors: Fatemipour M, Arabzadeh SAM, Molaei H, Geramizadeh B, Dabiri S, Fatemipour B, Vahedi SM, Malekpour Afshar R Abstract Chronic infection with hepatitis B (CHB) virus is one of the most important risk factors for Hepatocellular carcinoma (HCC). Hepatitis B virus (HBV) causes liver cancer in various ways. One of these ways is increasing the expression of the signal transducer and activator of transcription 3 (STAT3) in Hepatocytes by HBV. The purpose of this research is to evaluate the single nucleotide polymorphism (SNP) rs1053004 in the STAT3 gene in CHB patients and individuals who suffer from HCC. In this ...
Source: Cellular and Molecular Biology - January 10, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Evaluation of protein levels of autophagy markers (Beclin 1 and SQSTM1/p62) and phosphorylation of cyclin E in the placenta of women with preeclampsia.
Authors: Akcora Yildiz D, Irtegun Kandemir S, Agacayak E, Deveci E Abstract Preeclampsia is a severe multisystem disorder, and its pathophysiology is still not completely understood. Autophagy, a recycling process that maintains cellular homoeostasis during differentiation and development, is controversial regarding increased or decreased autophagic activity in preeclampsia. The aim of this study was to determine whether autophagy is increased in the placentas of women with preeclampsia by examining the protein levels of autophagy markers (Beclin 1 and SQSTM1/p62) and phosphorylation of cyclin E. For this purpose, ...
Source: Cellular and Molecular Biology - January 10, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Hepatoprotective properties for Salvia cryptantha extract on carbon tetrachloride-induced liver injury.
Authors: Yalcin A, Yumrutas O, Kuloglu T, Elibol E, Parlar A, Yilmaz İ, Pehlivan M, Dogukan M, Uckardes F, Aydin H, Turk A, Uludag O, Sahin İ, Ugur K, Aydin S Abstract The present study was designed to determine the possible hepatoprotective effects of Salvia cryptantha (black weed) plant extract against carbon tetrachloride (CCl4)-induced hepatic injury in rats. Animals were grouped as follows: control group (Group I), CCl4 group (Group II), olive oil group (Group III), CCl4 + S. cryphantha 200 mg/kg group (Group IV), and CCl4 + S. cryptantha 400mg/kg group (Group V). Rats...
Source: Cellular and Molecular Biology - January 10, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Molecular and phenotypic characterization of the vancomycin-resistant gene in bacterial isolates acquired from catheter tips.
Authors: Siddiqui S, Saeed M, Faisal SM, Akhtar A, Sarim KM, Khan M, Ahmad I, Ahmad I Abstract Gram-positive bacteria, particularly Staphylococcus aureus is a significant pathogen, not only in the hospital setting but the community also. S. aureus is a major cause of serious hospital and community-acquired infections, particularly in the colonized individuals. The emergence of vancomycin-resistant S. aureus (VRSA) strains has led to global concerns about treatments for staphylococcal infections. Until now, few strains of VRSA have been reported worldwide. The conventional disk diffusion method for determination of ...
Source: Cellular and Molecular Biology - January 10, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Regulation of signal transduction cascades by Pterostilbenes in different cancers: Is it a death knell for oncogenic pathways.
Authors: Butt G, Attar R, Tabassum S, Aras A, Qadir MI, Ozbey U, Alaaeddine N, Ozcelik B, Farooqi AA Abstract Interdisciplinary research has revolutionized the field of medicine and we have witnessed exponential increase in the high-impact research in past few decades. However, the road to this burgeoning research field is obstacle-ridden because of intratumor heterogeneity, loss of apoptosis and dysregulation of spatio-temporally controlled signaling pathways. Ground-breaking findings obtained through genetic, genomic and proteomic studies have considerably improved our concepts related to the complexity of protei...
Source: Cellular and Molecular Biology - January 10, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Astemizole inhibits cell proliferation in human prostate tumorigenic cells expressing ether à-go-go-1 potassium channels.
Astemizole inhibits cell proliferation in human prostate tumorigenic cells expressing ether à-go-go-1 potassium channels. Cell Mol Biol (Noisy-le-grand). 2017 Dec 17;63(12):11-13 Authors: Bernal-Ramos G, Hernández-Gallegos E, Vera E, Chávez-López MG, Zúñiga-García V, Sánchez-Pérez Y, Garrido E, Camacho J Abstract Prostate cancer (PC) is the main cause of cancer mortality in men worldwide. Therefore, novel treatments for PC are needed. Ether à-go-go-1 (Eag1) potassium channels display oncogenic properties, and have been suggested as e...
Source: Cellular and Molecular Biology - January 10, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Nanobiotechnology: A promising scope of gold biotechnology.
Authors: Barabadi H PMID: 29307347 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - January 10, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Biochemical evaluation of hydroxyurea derivative schiff bases in liver of rats.
In conclusion, it can be suggested that the determination of the pharmacological characteristics of them can be beneficial in numerous fields of application thanks to the antioxidant and hepatotoxic activities demonstrated by hydroxyurea derivative Schiff bases. PMID: 29208168 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - December 8, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research