Induced dedifferentiation of barley (Hordeum vulgare L.) embryonic cells and its relationship with agronomic traits.
This study was carried out to investigate the response of 42 Iranian and European barley (Hordeum vulgare L.) cultivars to induced dedifferentiation of embryonic cells via immature embryo culture and understand the relationship between embryo culture characters and agronomic traits. The cultivars were evaluated for dedifferentiation of embryonic cells or callus induction from immature embryo culture based on a completely randomized design with unequal replication. Immature embryos were placed scutellum down on cell dedifferentiation medium based on MS and supplemented with 2.5 mg/l 2,4-D. The developed calli were transferr...
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effect of pomegranate (Punica granatum L.) juice on kidney, liver, heart and testis histopathological changes, and the tissues lipid peroxidation and antioxidant status in lead acetate-treated rats.
This study investigated the protective potential of PJ against lead-induced oxidative stress. A total of forty adult male Sprague Dawley rats were divided into four experimental groups. The animals were fed a standard pellet diet and tap water ad libitum. The rats were divided into four groups (n=10 for each group): control, lead asetat (2000 ppm), low-treated PJ- a daily dose of 2.000 ppm lead plus 30µl pomegranate juice (included 1.050 µmol total polyphenols, gallic acid equivalent), and high-treated PJ- a daily dose of 2.000 ppm lead plus 60µl pomegranate juice (included 2.100 µmol total polyphen...
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

HMGCR and ApoE mutations may cause different responses to lipid lowering statin therapy.
The objective of this study was to investigate whether common variations in HMG-CoA Reductase(HMGCR) and Apolipoprotein E (ApoE) genes involved in lipid and statin metabolism modify the effect of statins on serum lipid and lipoprotein concentrations in CAD patients.A hundred  CAD patients were enrolled into the study. At the beginning of the study biochemical measurements were performed to determine the baseline levels performed. Patients were treated with 40 mg atorvastatin for 2 months and biochemical measurements were repeated. According to the post-treatment, LDL-c levels,  patients were divided into 2 groups...
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Cardioprotective effect of Pycnogenol in ischemic-reperfusion injury (IRI) in rats.
Authors: Xiao P, Zhang K, Tao Z, Liu N, Ge B Abstract Oxidative stress plays a critical task in the biochemical and pathological alteration linked with myocardial ischemic-reperfusion injury (IRI). This warrants identifying agents with a potential for preventing such damage in an effective way. A novel plant based product, Pycnogenol, obtained from the French maritime pine (Pinus pinaster ssp. atlantica) bark extract was known for its tremendous antioxidant potential (both in vivo, in vitro). It was able to attenuate the symptoms of immune dysfunction through restoring a cellular antioxidant status in low micronutr...
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Hyperandrogenism stimulates inflammation and promote apoptosis of cumulus cells.
Authors: Xu F, Liu R, Cao X Abstract To explore roles of androgen and cytokines such as IL-1, IL-6 and TNF-α in PCOS. Cumulus cells were isolated from rat COCs and divided into two sets of experiments. Set 1 includes 4 groups: Control group, IL-1 group (final concentration at 5 nM), IL-6 group (final concentration at 5nM) and TNF-α group (final concentration at 5nM). Set 2 also include 4 groups: Control group, Androgen group 1 (final concentration at 1 nM), Androgen group 2 (final concentration at 10 nM) and Androgen group 3 (final concentration at 100 nM). Androgen, at final concentration of 100 nM, ca...
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Application of unilateral multiple channels approach in percutaneous vertebroplasty for osteoporotic vertebral fractures.
This study aimed to investigate the application valuable of percutaneous vertebroplasty (PVP) with unilateral multiple channels approach in osteoporotic vertebral fractures patients. A retrospective review was conducted on 685 consecutive patients with osteoporotic vertebral fracture from March 2003 to October 2012. Among them, 82 cases were given PVP procedure by unilateral multiple channels approach. The timing of surgery, bone cements injection, and complications were analyzed. By using the X-ray film, the distribution of cement was detected and scored. The visual analogue scale (VAS) score and Oswestry disability index...
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Overexpression of microRNA-133b reduces myocardial injuries in children with viral myocarditis by targeting Rab27B gene.
Authors: Zhang Y, Sun L, Sun H, Liu X, Luo X, Li C, Sun D, Li T Abstract The present study is to measure the expression of microRNA (miRNA or miR)-133b in circulating blood of children with viral myocarditis before and after drug treatment, and to investigate its relationship with the severity of myocardial lesions. A total of 36 children patients with viral myocarditis who received treatments at our hospital between June 2014 and June 2016 were enrolled in the present study, including 21 boys and 15 girls (age range, 9 months - 16 years).Quantitative real-time polymerase chain reaction was used to determine the ex...
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Investigation of genotoxic effects of doripenem using cytogenetic and molecular methods.
In this study, according to the obtained results, it was determined that DRP failed to show any genotoxic risk at the therapeutic doses. This result also indicates that DRP could be a reliable antibiotics according to its rapid metabolism. PMID: 29096747 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Organic chromium modifies the expression of orexin and glucose transporters of ovarian in heat-stressed laying hens.
In conclusion, CrPic and CrHis showed supported the relief and treatment of stress complications. PMID: 29096748 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

A bioinformatics approach for identification of miR-100 targets implicated in breast cancer.
Authors: Shamsi R, Seifi-Alan M, Behmanesh A, Omrani MD, Mirfakhraie R, Ghafouri-Fard S Abstract MicroRNAs (miRNAs) are small endogenous non-coding RNAs with principal roles in regulation of protein expression via translation repression and mRNA degradation. Based on these roles they are implicated in tumourigenesis processes as well. Among them is miR-100 which can exert both tumor suppressor and oncogenic functions in various cancer types. In breast cancer, it has been shown to affect apoptosis, epithelial-mesenchymal transition as well as tumor-related signaling pathways. In the present study, we introduce a nov...
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Clinical efficacy and mechanism of lymphoplasma exchange in the treatment of Guillain-Barre syndrome.
Authors: Luo MC, Wang WF, Yin WF, Li Y, Li BJ, Duan WW, Zeng QM, Luo YB, Yang XS, Yang H Abstract Guillain-Barre syndrome (GBS) is an autoimmune disease of the nervous system and is the most common acute polyneuropathy. Both cellular and humoral immunity are believed to be involved in the pathogenesis of GBS, and various types of activated CD4+ T cells are thought to orchestrate the onset and progression of GBS. Lymphoplasma exchange (LPE) filtering out activated lymphocytes while exchanging plasma has been used for GBS treatment for years. However the treatment is still not yet optimal. In order to assess the effi...
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

LPS-induced Src family kinases activity mediates IL-10 production through activation of STAT3 in peripheral blood mononuclear cells of patients with Beh çet's Disease.
LPS-induced Src family kinases activity mediates IL-10 production through activation of STAT3 in peripheral blood mononuclear cells of patients with Behçet's Disease. Cell Mol Biol (Noisy-le-grand). 2017 Oct 31;63(10):116-121 Authors: Irtegun Kandemir S, Tekin MA, Bozkurt M, Dagli AZ, Kalkanli-Tas S Abstract Behçet's disease (BD) is achronic inflammatory disorder characterized by recurrent oral and genital ulcers, uveitis and skin lesions. Although,the pathogenesis of BD remains poorly understood, excessive or dysregulatedcytokine production including IL-10 is associated with BD. Revealin...
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The association between murine double minute 2 (MDM2) rs2279744 and endometrial cancer risk in a Chinese Han population.
In conclusion, the study found that MDM2 rs2279744 polymorphism was significantly associated with endometrial cancer risk in a Chinese Han population. PMID: 29096752 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Interleukin-6 secreted by oral cancer- associated fibroblast accelerated VEGF expression in tumor and stroma cells.
In this study, we examined the correlation between IL-6 and CAF and the role of this correlation on VEGF production. In this study, quantitative expression level of IL-6 and VEGF in CAF and Oral Cancer Cells (OCCs) examined through Real Time PCR and ELISA and western blot analysis. In addition, maintenance and retention of IL-6 and VEGF checked out in co-culture experiment of CAF and OCC cells. These experiments demonstrated that in oral cancer, CAF cell line secretes significantly more IL-6 than OCC. Also IL-6 is a factor that causes VEGF secretion in CAF cell line. CAF is the basic and the most essential source for produ...
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Overview of ribosomal and non-ribosomal antimicrobial peptides produced by Gram positive bacteria.
Authors: Tajbakhsh M, Karimi A, Fallah F, Akhavan MM Abstract The increasing incidence of antimicrobial resistance bacterial infection and decreasing effectiveness of conventional antibiotics to treatment have caused serious problems worldwide. The demand for new generationantibiotics to combat microbial pathogens is imperative. Cationic antimicrobial peptides (AMPs) with different sources from prokaryotic to complex eukaryotic organisms, with variable length, amino acid composition and secondary structure, have been consideredduring the past decades.  The advantages of large number of AMPs are related to broa...
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Ziziphus Jujube: a review study of its anticancer effects in various tumor models invitro and invivo.
Authors: Ebrahimi S, Mollaei H, Hoshyar R Abstract The growing rate of cancer incidences and inefficiency of current therapies have made scientists to find novel agents such as medicinal plants. Ziziphus jujube is one of these plants whose pharmaceutical effects have been studied for a long time. Recent investigations have proved that bioactive compounds of Ziziphus jujube including triterpenic acids, flavonoids, cerebrosides, phenolic acids, α tocopherol, β carotene, and polysaccharides have anti-proliferative effects on several cancerous cell lines and animals. The present study aimed to cover all prev...
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Genetic barcoding and phylogenetic analysis of dusky cotton bug (Oxycarenus hyalinipennis) using mitochondrial cytochrome c oxidase I gene.
Authors: Khan AZ, Amad I, Shaheen S, Hussain K, Hafeez F, Farooq M, Noor Ul Ayan H Abstract Cotton dusky bug (Oxycarenus spp.) mostly attack on cash crops such as Gossypium, Cola and Hibiscus which affect the national economy therefore sustainable pest management is needed. Cytochrome c oxidase I (COI) gene is utilized as marker gene for DNA barcoding, genetic and ecological study of insects. In present study insect (cotton dusky bug) samples were collected from cotton fields in Faisalabad. COI gene was amplified from genomic DNA of bug and cloned into pTZ57R/T vector (Fermentas). The clone was sent to Macrogen (So...
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Matrix metalloproteinase-1 (MMP-1) rs1799750 polymorphism is associated with nasopharyngeal carcinoma (NPC) risk.
In conclusion, this meta-analysis showed that MMP-1 rs1799750 polymorphism was significantly associated with NPC risk. PMID: 29096757 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Endothelial nitric oxide synthase 27VNTR (4b/4a) gene polymorphism and the risk of diabetic microvascular complications in Chinese populations.
Authors: Zhang X, Yang Z, Chen X Abstract To access the association of endothelial nitric oxide synthase (eNOS) gene 27VNTR (4b/4a) polymorphism with diabetic microvascular complications (DMI) susceptibility in Chinese populations. Data were retrieved in a systematic manner on PubMed database, ISI Web of Knowledge, the Cochrane Library, Chinese National Knowledge Infrastructure, and Wanfang, as well as manual searching of the references of the identified articles. Odds ratio (OR) and 95% confidence interval were used to evaluate the strength of associations. Potential sources of heterogeneity and publication bias w...
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Circulating macrophage migration inhibitory factor levels and its polymorphisms in systemic lupus erythematosus: A meta-analysis.
Authors: Bae SC, Lee YH Abstract The present study aimed to systemically review the evidence regarding the relationship between circulating macrophage migration inhibitory factor (MIF) levels and systemic lupus erythematosus (SLE), as well as the associations between several polymorphisms in the MIF gene and SLE susceptibility. We performed a meta-analysis of serum/plasma levels of MIF in SLE patients and controls and evaluated evidence of associations between the MIF -173 C/G allele and -794CATT5-8 polymorphisms and the associated risk for SLE. Nine studies were included in this meta-analysis. Meta-analysis indica...
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

ALOX5AP rs10507391 polymorphism and the risk of ischemic stroke in Caucasians: an update meta-analysis.
In conclusion, this study found that ALOX5AP rs10507391 polymorphism was associated with ischemic stroke risk in Caucasians. PMID: 29096760 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - November 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Bioactive chemical compounds in Eremurus persicus (Joub. & Spach) Boiss. essential oil and their health implications.
In conclusion, our results suggested that this EO possesses a wide range of bioactive properties that could be useful in nutraceutical, functional foods and cosmeceutical formulations. PMID: 28980914 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - October 6, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Functional role of inflammation in the surgical injury induced vascular remodeling of male albino rats.
Authors: Song X, Li L, Wu Y, Zhang L, Li X Abstract Our present study investigates the cellular and molecular inflammatory events in male albino rat arterial injury. Male albino rats were subjected to longitudinal incision and carotid artery clamping for the duration of 45 days. Heat shock protein (HSP) 27, HSP70, HSP47 and Nuclear Factor kappa B (NF-κB) expressions were determined by qPCR and Western blot method. The morphology of vessel wall alteration was studied by the light microscopy. The expression of NF-κB was found to be increased after ten days of carotid artery injury. The qPCR and Western bl...
Source: Cellular and Molecular Biology - October 6, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Cetuximab has an inhibitory effect on cell motility in SCC-4 oral squamous cell carcinoma cell line.
This study aimed to evaluate the effect of treatment with Cetuximab on cell migration and invasion in OSCC cells, by using the SCC-4 cell line. Cell migration and cell invasion assay were performed and actin cytoskeleton of control and treated with Cetuximab cells were evaluated. Differences were considered significant when p<0.05.Cetuximab inhibited the migration of SCC-4 cells at three concentrations: 1 µg/mL, 50 µg/mL and 100 µg/mL (p<0.0001) in a dose-dependent manner. The number of SCC-4 treated cells with 1 μg/mL that migrated through the membrane was statistically different from 50...
Source: Cellular and Molecular Biology - October 6, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

FAM196B acts as oncogene and promotes proliferation of gastric cancer cells through AKT signaling pathway.
Authors: Zhang J, Tong DD, Xue M, Jiang QY, Wang XF, Yang PB, Ni L, Zhao LY, Huang C Abstract Gastric cancer (GC) is the second leading cause of cancer-related deaths worldwide, but the mechanisms remain unknown. Here we report that family with sequence similarity 196 member B (FAM196B) is highly expressed in primary GC tissues and the expression level is correlated with the clinicopathologic characteristics of GC. In this experiment, knockdown of FAM196B suppressed GC cell proliferation and induced G1/G0 to S phase cell cycle arrest by regulating Cyclin D1, Cyclin A and CDK2 expressions. Furthermore, we investigat...
Source: Cellular and Molecular Biology - October 6, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Some heavy metals in Luciobarbus esocinus for public consumption and consumer protection.
In this study, the concentrations of some heavy metals were determined in the muscle of Luciobarbus esocinus Heckel, 1843 and in water where they live. The results were evaluated in term of potential human risk of consumption of this fish and the relationship between the heavy metal load of fish and some of their biological aspects (weight, length and sex). In addition, the accumulation factor of heavy metals in the muscle of L. esocinus were also determined. The Cr, Pb and Ni were not found in detectable levels according to results obtained by ICP. Only Cu, Zn and Fe were detected. It was found that heavy metals concentra...
Source: Cellular and Molecular Biology - October 6, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Risk assessment of a formamidine pesticide, Amitraz, focusing on thyroid hormone receptors (TRs) in rainbow trout, Oncorhynchus mykiss.
Authors: Meriç Turgut I, Keskin E Abstract Amitraz, a formamidine pesticide, and their metabolites have the potential to disrupt endocrine homeostasis in a variety of organisms, nevertheless there is a lack of information concerning such effects and underlying mechanisms in any fish species.To evaluate the potential impacts of Trasil (EC; active constituent 200 g amitraz/L), a commercial product of amitraz, on thyroid hormone (TH) homeostasis of rainbow trout (Oncorhynchus mykiss); mRNA levels of thyroid hormone receptors (TRs), TRα and TRβ, were determined by RT-PCR soon after sub-lethal administ...
Source: Cellular and Molecular Biology - October 6, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Transcriptomic analysis of human non-small lung cancer cells A549 treated by one synthetic curcumin derivative MHMD.
Authors: Zhou G, Sun G, Zhou Y, Wang Q Abstract In our previous studies, we have identified one curcumin analog MHMD could induce apoptosis of lung cancer cells A549 via extrinsic and intrinsic pathways in our previous studies. But the specific regulatory genes and molecular mechanisms remain poorly understood. Here, the transcriptomic profile of A549 cells was detected with RNA-seq technique after MHMD treatment at 48 h. A total of 16584651 clean data from 21831774 sequence reads were obtained and 80.75% of them could be mapped on the human test genome. 18635 unigenes with the mean length of 4027 bp were finally a...
Source: Cellular and Molecular Biology - October 6, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Protease sensitivity and redistribution of CD71 and glycophorin A on K562 cells.
Authors: Yu CH, Wang H, Wang Y, Cui NX, Zhao X, Rong L, Yi ZC Abstract Transmembrane proteins are delivered to plasma membrane from the endoplasmic reticulum and Golgi complex by vesicular transport along with the cytoskeletal network. Disruption of this process likely affects transmembrane protein expression. K562 cells were digested with Streptomyces griseus protease for different periods of time, and then re-cultured with different cytoskeletal and glycosylation inhibitors. Cell viability and surface expression of transferrin receptor (CD71) and glycophorin A (GPA) were analyzed before and after re-culture by fl...
Source: Cellular and Molecular Biology - October 6, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

rs3918242 variant genotype frequency and increased TIMP-2 and MMP-9 expression are positively correlated with cancer invasion in urinary bladder cancer.
Authors: Pençe S, Özbek E, Ozan Tiryakioğlu N, Ersoy Tunali N, Pençe HH, Tunali H Abstract To study the role of MMP9 and TIMP2 genotypes and expression in predisposition to bladder cancer and relation with metastasis. 100 urinary bladder cancer patients and 100 healthy controls were included in the study. rs3918242 and rs8179090 genotypes were determined with PCR-RFLP. Quantitative real-time polymerase chain reaction was employed to assess the MMP-9 and TIMP-2 expression in tumors and adjacent healthy tissues. Variant genotype (TT) for rs3918242 polymorphism and rs8179090 variant genotype a...
Source: Cellular and Molecular Biology - October 6, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Morphological, genetic and pigment diversity of Nerium indicum Mill in Iran.
Authors: Mohammadi F, Sharifi-Sirchi G, Samsampour D Abstract The Nerium indicum Mill organs and parts of the 40 genotypes were sampled from 5 habitats of southand south east of Iran. In total, 15 morphological and pigmentvariables were measured. Analysis of variance was carried out based on completely randomized design and revealed significance differencesat p ≤ 0.05, 0.01 and 0.001 for the most variablesindicating a large-scale diversity among the genotypes. Cluster analysis divided the genotypes into 3 main groups. The first and second principal components had 34.73% and 18.67% of the variance, respectively. ...
Source: Cellular and Molecular Biology - October 6, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

STAT3 regulates cytokine expression in peripheral blood mononuclear cells from asthma patients.
Authors: Yang Q, Xu W, Long Y, Kuang J, Li J Abstract Asthma is a common long term inflammatory disease of the airways. This disease affected millions of people worldwide. Recently, it is demonstrated that signal transducer and activator of transcription 3 (STAT3) plays critical role in asthma occurrence. In the current study, we isolated peripheral blood mononuclear cells (PBMCs) from patients with mild and moderate asthma, and then determined the correlation between STAT3 and cytokine expression. We found that the concentration and mRNA level of cytokines was increased in PBMCs from asthma patients. The concentra...
Source: Cellular and Molecular Biology - October 6, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

miR-214 modulates cisplatin sensitivity of osteosarcoma cells through regulation of anaerobic glycolysis.
In this study, we report miR-214 contributes to cisplatin resistance in osteosarcoma cells. Overexpression of miR-214 decreased the cisplatin sensitivity. By establishing an osteosarcoma cisplatin resistant cell line, we find miR-214 is significantly upregulated in cisplatin resistant cells. Moreover, we show miR-214 promotes anaerobic glycolysis rates of osteosarcoma cells but suppresses mitochondrial oxidative phosphorylation. Consistently, cisplatin resistant cells exhibit upregulated glycolysis but decreased mitochondrial oxidative phosphorylation, a phenotype called "Warburg effect". Finally, we demonstrate ...
Source: Cellular and Molecular Biology - October 6, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Insufficient decontamination in Sewage treatment plants induce the risk of artificial selection of extended-spectrum β-lactamase producing Escherichia coli.
Insufficient decontamination in Sewage treatment plants induce the risk of artificial selection of extended-spectrum β-lactamase producing Escherichia coli. Cell Mol Biol (Noisy-le-grand). 2017 Sep 30;63(9):80-86 Authors: Gundogdu A, Kilic H, Kurekci C, Alp E Abstract Quantitative data about extended-spectrum beta-lactamase producing E. coli (ESBLEC) in the wastewaters are scarce, especially in developing countries. These data could be useful to raise awareness about the potential risk of spreading ESBLEC strains in the community. Water samples were collected weekly over a 10-week period, from one...
Source: Cellular and Molecular Biology - October 6, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Antioxidant activities, anticancer activity and polyphenolics profile, of leaf, fruit and stem extracts of Pistacia lentiscus from Tunisia.
Authors: Yemmen M, Landolsi A, Ben Hamida J, Mégraud F, Trabelsi Ayadi M Abstract Plant derived compounds have played an important role in the development of several clinically useful anticancer drugs. The aim of the present study was 1) to evaluate for the first time the anti-proliferative activity of a polyphenol enriched extract obtained from leaf, fruit and stem of Tunisian variety of Pistacia lentiscus against two cultured cancer cells, and 2) to carry out a phytochemical analysis of vegetable extracts particularly by determining the chemical composition of phenolics (total polyphenols, flavonoids and c...
Source: Cellular and Molecular Biology - October 6, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Optimizing expression of antiviral cyanovirin-N homology gene using response surface methodology and protein structure prediction.
Authors: Lotfi H, Hejazi MA, Heshmati MK, Mohammadi SA, Zarghami N Abstract Cyanovirin-N (CVN) is well known as an anti-HIV protein. The efficient production of low cost microbicides for preventing the HIV-infection  has lately become a requirement worldwide. The aim of the present study was to optimize the expression of antiviral Cyanovirin-N homology gene found in the indigenous strain of Nostoc ellipsospourum LZN using Response Surface Methodology (RSM) and Protein Structure Analysis. Optimization of three induction factors (IPTG concentration (0.1, 0.55 and 1mM), temperature for bacterial growth (20, 28.5 ...
Source: Cellular and Molecular Biology - October 6, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Cloning and expression of rice glutamate decarboxylase (GAD) in Escherichia coli.
In this study, rice GADs was cloned in E.coli in both 37°C and 28°C. Two concentrations of Isopropyl-β-D-thiogalactoside (IPTG) (0.5mM and 1mM) were investigated in TB medium. Purification was done with Ni2+-nitrilotriacetic acid (NTA) resin in various concentration of imidasol. According to SDS-PAGE analysis, rice GADs was cloned and expressed successfully in E.coli MC 1061 bacterium and the most expression was done in 37°C and 0.5mM IPTG and the best concentration of imidasol was 100mM for purification step. Based on the results, rice GADs can be expressed in E.coli MC 1061 bacterium and, hence, it is a ...
Source: Cellular and Molecular Biology - October 6, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Study of anticancer and antibacterial activities of Foeniculum vulgare, Justicia adhatoda and Urtica dioica as natural curatives.
Authors: Batool R, Salahuddin H, Mahmood T, Ismail M Abstract High-throughput technologies, such as synthetic biology and genomics have paved new paths for discovery and utility of medicinally beneficial plants. Bioactive molecules isolated from different plants have significantly higher biological activities. The present study was done to analyze antibacterial potential of some medicinal plants against multi drug resistant (MDR) pathogens and anticancer effect against MCF-7 cell line. Methanolic and ethanolic extracts were tested for their antibacterial activity by disc diffusion method against six MDR bacterial s...
Source: Cellular and Molecular Biology - October 6, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Bacterial etiology and antibiotic resistance pattern of septicemia in HIV and non-HIV patients admitted to tertiary care hospitals, Shiraz, South of Iran.
Authors: Ghassabi F, Hashempour T, Moghadami M, Davarpanah MA, Kalani M, Chatrabnous N, Halaji M, Shahraki HR, Hadi N Abstract The present study aimed to determine the bacteriological etiology and antibiotic susceptibility pattern of sepsis in HIV infected and HIV uninfected patients, and related risk factors to introduce an appropriate therapy. This cross-sectional study was conducted from January 2014 to January of 2015 enrolling patients with sepsis associated with or without HIV infection admitted to Shiraz teaching hospitals, South of Iran. Blood and urine cultures were performed and standard microbiological m...
Source: Cellular and Molecular Biology - October 6, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Estimating the modulatory effect of cadmium chloride on the genotoxicity and mutagenicity of silver nanoparticles in mice.
Authors: Mohamed HRH Abstract Silver (Ag) nanoparticles (nano-Ag) are widely used because of their distinctive antimicrobial properties, but this widespread use increases Ag release into the environment along with many other pollutants such as heavy metals. Therefore, this study was undertaken to study the modulatory effect of cadmium chloride (CdCl2) on the genotoxicity and mutagenicity of nano-Ag in mice liver, kidney and brain tissues. Co-injections of CdCl2 (1.5 mg/kg) with nano-Ag (20, 41, or 82 mg/kg) resulted in significant elevations in both single and double DNA strand breaks that triggered higher apoptoti...
Source: Cellular and Molecular Biology - October 6, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Predictive role of cytokines IL-10, IL-12 and TNF- α gene polymorphisms for the development of osteonecrosis of the femoral head in the Chinese Han population.
Predictive role of cytokines IL-10, IL-12 and TNF-α gene polymorphisms for the development of osteonecrosis of the femoral head in the Chinese Han population. Cell Mol Biol (Noisy-le-grand). 2017 Sep 30;63(9):144-149 Authors: Yuan L, Li W, Tian ZB, Li Y, Sun S Abstract The progressive neurodegenerative process in osteonecrosis of the femoral head (ONFH) is accompanied by chronic inflammation, including activation of microglia and astrocytes that express pro-inflammatory cytokines. Up to now, numerous studies of genetic epidemiology have assessed the association of anti- and pro-inflammation cytok...
Source: Cellular and Molecular Biology - October 6, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Regulation of signaling pathways by tanshinones in different cancers.
Authors: Lin X, Qureshi MZ, Romero MA, Khalid S, Aras A, Ozbey U, Farooqi AA Abstract Past several years have witnessed dramatic leaps in our understanding of rewiring of gene expression at the translation level during cancer developmentthat provides linchpin support to the transformed phenotype. Most recent and ground-breaking developments in the field of molecular oncology aredriven by an explosion in technological advancements and have started to reveal previously unimagined regulatory mechanisms and how they intricately co-ordinate to modulate cancer progression, loss of apoptosis and development of resistance ...
Source: Cellular and Molecular Biology - October 6, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Lactobacillus acidophilus versus placebo in the symptomatic treatment of irritable bowel syndrome: the LAPIBSS randomized trial.
Authors: Sadrin S, Sennoune SR, Gout B, Marque S, Moreau J, Grillasca J, Pons O, Maixent JM Abstract Irritable bowel syndrome is a chronic functional gastrointestinal disorder characterized by abdominal pain/discomfort and altered bowel habits. The use of Lactobacilli as probiotics during irritable bowel syndrome is based on their interesting mechanisms of action and their excellent safety profile but little is known about their clinical efficacy due to the lack of adequately designed clinical trials. The current clinical trial protocol aims to determine the effects of a mixture of Lactobacillus acidophilus NCFM an...
Source: Cellular and Molecular Biology - October 6, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Null genotype of GSTT1 may be associated with esophageal cancer risk: a meta-analysis in Chinese individuals.
This study suggested that GSTT1 null genotype may be potential biomarkers for esophageal cancer in China, especially in North China. Studies with larger sample sizes and wider spectrum of populations are warranted to verify this finding. PMID: 28980936 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - October 6, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

MiR-21 functions oppositely in proliferation and differentiation of neural stem/precursor cells via regulating AKT and GSK-3 β.
In this study, we demonstrated that miR-21 reduced proliferation and promoted neural differentiation in NSPCs via regulating the activation of AKT and GSK-3β signaling pathways in vitro. During differentiation of NSPCs, the expression of miR-21 was increased in a time-dependent manner by qRT-PCR. Synthesized pre-miR-21 or anti-miR-21 was transfected into NSPCs, thereby efficiently overexpressing or knocking down miR-21. Overexpression of miR-21 promoted the neural differentiation of NSPCs, as indicated by Tuj1 and PSA-NCAM staining. Interestingly, knocking down miR-21 had the opposite effect of neural differentiation ...
Source: Cellular and Molecular Biology - October 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effects of alpha lipoic acid on acrylamide-induced hepatotoxicity in rats.
This study was carried out to study the protective effect of alpha lipoic acid on acrylamide induced perturbations in rat liver.  Four groups of rats were studied viz., control rats, acrylamide treated rats, alpha lipoic acid treated rats, and alpha lipoic acid plus acrylamide treated rats. ACR and ALA treatment alone and together caused a signifi-cant increase in hepatic reduced glutathione content while a decrease in hepatic ascorbic content was observed when compared to control group.  ALA pretreatment of acrylamide exposed rats caused no a signifi-cant alteration in superoxide dismutase activity but resulted ...
Source: Cellular and Molecular Biology - October 3, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Evaluation of the nutrient profile of Trachyspermum ammi L. seed under the influence of nanoparticles during germination.
This study aimed to provide new insight of the potential growth promoting effects of the nanoparticles () on plant system. Different concentrations of two nanoparticles, that is, iron pyrite (FeS2) and molybdenum disulphide (MoS2) at three different concentrations of 25ug/ml, 50ug/ml and 75ug/ml were tested on the seeds of Trachyspermum ammi L. The data indicated that nanoparticles enhanced the seedling growth as greener leafs and increased lengths of epicotyl and hypocotyls were seen. These nanoparticles also showed the potential to increase the contents of primary metabolites during germination and the total soluble prot...
Source: Cellular and Molecular Biology - October 3, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Curcumin mediated attenuation of carbofuran induced toxicity in the heart of Wistar rats.
Authors: Jaiswal SK, Gupta VK, Siddiqi NJ, Sharma B Abstract Carbofuran is used to improve the agricultural productivity as well as to protect the house hold and industrial products, but due to accumulation in the biological system, it causes serious side effects in many non-targets mammalian systems. The aim of present study is to evaluate the carbofuran induced oxidative stress in rat heart and its attenuation by using herbal product curcumin. Rats were divided into four groups; one group received 20 % LD50 of carbofuran another group of rats received same doses of carbofuran was  pretreated with curcumin (1...
Source: Cellular and Molecular Biology - October 3, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Synthesis, characterization of nano-sized anatase TiO2   and its adsorption behaviour  for environmental contaminant.
Synthesis, characterization of nano-sized anatase TiO2  and its adsorption behaviour  for environmental contaminant. Cell Mol Biol (Noisy-le-grand). 2017 Jul 31;63(6):18-20 Authors: Gupta K, Pandey A, Singh RP Abstract TiO2 is one of the most studied material due to its unique properties like chemical stability, non toxicity and cost effectiveness.  Nano-sized TiO2   has been prepared by sol -gel method using titanium butoxide as precursor. X- ray diffraction analysis was used to characterize the phase and crystal size of the sy...
Source: Cellular and Molecular Biology - October 3, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Prevalence of COMT Val158Met polymorphism in Eastern UP population.
Authors: Kumar P, Yadav U, Rai V Abstract Catechol-O-methyltransferase (COMT) is an abundant S-adenosylmethionine (SAM-)-dependent methyltransferase that methylates catechol compounds, including catecholamines and catecholestrogens.COMT  gene located at chromosome 22q11.2 contains a functional polymorphism at codon 158(Val158Met), which has been related to psychiatric diseases and different types of cancer. COMT might affect tHcy levels because as a by-product it converts SAM to S-adenosylhomocysteine (SAH), which is reversibly converted to homocysteine. The aim of the present study was to determine the freque...
Source: Cellular and Molecular Biology - October 3, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research