Optimizing expression of antiviral cyanovirin-N homology gene using response surface methodology and protein structure prediction.
Authors: Lotfi H, Hejazi MA, Heshmati MK, Mohammadi SA, Zarghami N Abstract Cyanovirin-N (CVN) is well known as an anti-HIV protein. The efficient production of low cost microbicides for preventing the HIV-infection  has lately become a requirement worldwide. The aim of the present study was to optimize the expression of antiviral Cyanovirin-N homology gene found in the indigenous strain of Nostoc ellipsospourum LZN using Response Surface Methodology (RSM) and Protein Structure Analysis. Optimization of three induction factors (IPTG concentration (0.1, 0.55 and 1mM), temperature for bacterial growth (20, 28.5 ...
Source: Cellular and Molecular Biology - October 6, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Cloning and expression of rice glutamate decarboxylase (GAD) in Escherichia coli.
In this study, rice GADs was cloned in E.coli in both 37°C and 28°C. Two concentrations of Isopropyl-β-D-thiogalactoside (IPTG) (0.5mM and 1mM) were investigated in TB medium. Purification was done with Ni2+-nitrilotriacetic acid (NTA) resin in various concentration of imidasol. According to SDS-PAGE analysis, rice GADs was cloned and expressed successfully in E.coli MC 1061 bacterium and the most expression was done in 37°C and 0.5mM IPTG and the best concentration of imidasol was 100mM for purification step. Based on the results, rice GADs can be expressed in E.coli MC 1061 bacterium and, hence, it is a ...
Source: Cellular and Molecular Biology - October 6, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Study of anticancer and antibacterial activities of Foeniculum vulgare, Justicia adhatoda and Urtica dioica as natural curatives.
Authors: Batool R, Salahuddin H, Mahmood T, Ismail M Abstract High-throughput technologies, such as synthetic biology and genomics have paved new paths for discovery and utility of medicinally beneficial plants. Bioactive molecules isolated from different plants have significantly higher biological activities. The present study was done to analyze antibacterial potential of some medicinal plants against multi drug resistant (MDR) pathogens and anticancer effect against MCF-7 cell line. Methanolic and ethanolic extracts were tested for their antibacterial activity by disc diffusion method against six MDR bacterial s...
Source: Cellular and Molecular Biology - October 6, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Bacterial etiology and antibiotic resistance pattern of septicemia in HIV and non-HIV patients admitted to tertiary care hospitals, Shiraz, South of Iran.
Authors: Ghassabi F, Hashempour T, Moghadami M, Davarpanah MA, Kalani M, Chatrabnous N, Halaji M, Shahraki HR, Hadi N Abstract The present study aimed to determine the bacteriological etiology and antibiotic susceptibility pattern of sepsis in HIV infected and HIV uninfected patients, and related risk factors to introduce an appropriate therapy. This cross-sectional study was conducted from January 2014 to January of 2015 enrolling patients with sepsis associated with or without HIV infection admitted to Shiraz teaching hospitals, South of Iran. Blood and urine cultures were performed and standard microbiological m...
Source: Cellular and Molecular Biology - October 6, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Estimating the modulatory effect of cadmium chloride on the genotoxicity and mutagenicity of silver nanoparticles in mice.
Authors: Mohamed HRH Abstract Silver (Ag) nanoparticles (nano-Ag) are widely used because of their distinctive antimicrobial properties, but this widespread use increases Ag release into the environment along with many other pollutants such as heavy metals. Therefore, this study was undertaken to study the modulatory effect of cadmium chloride (CdCl2) on the genotoxicity and mutagenicity of nano-Ag in mice liver, kidney and brain tissues. Co-injections of CdCl2 (1.5 mg/kg) with nano-Ag (20, 41, or 82 mg/kg) resulted in significant elevations in both single and double DNA strand breaks that triggered higher apoptoti...
Source: Cellular and Molecular Biology - October 6, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Predictive role of cytokines IL-10, IL-12 and TNF- α gene polymorphisms for the development of osteonecrosis of the femoral head in the Chinese Han population.
Predictive role of cytokines IL-10, IL-12 and TNF-α gene polymorphisms for the development of osteonecrosis of the femoral head in the Chinese Han population. Cell Mol Biol (Noisy-le-grand). 2017 Sep 30;63(9):144-149 Authors: Yuan L, Li W, Tian ZB, Li Y, Sun S Abstract The progressive neurodegenerative process in osteonecrosis of the femoral head (ONFH) is accompanied by chronic inflammation, including activation of microglia and astrocytes that express pro-inflammatory cytokines. Up to now, numerous studies of genetic epidemiology have assessed the association of anti- and pro-inflammation cytok...
Source: Cellular and Molecular Biology - October 6, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Regulation of signaling pathways by tanshinones in different cancers.
Authors: Lin X, Qureshi MZ, Romero MA, Khalid S, Aras A, Ozbey U, Farooqi AA Abstract Past several years have witnessed dramatic leaps in our understanding of rewiring of gene expression at the translation level during cancer developmentthat provides linchpin support to the transformed phenotype. Most recent and ground-breaking developments in the field of molecular oncology aredriven by an explosion in technological advancements and have started to reveal previously unimagined regulatory mechanisms and how they intricately co-ordinate to modulate cancer progression, loss of apoptosis and development of resistance ...
Source: Cellular and Molecular Biology - October 6, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Lactobacillus acidophilus versus placebo in the symptomatic treatment of irritable bowel syndrome: the LAPIBSS randomized trial.
Authors: Sadrin S, Sennoune SR, Gout B, Marque S, Moreau J, Grillasca J, Pons O, Maixent JM Abstract Irritable bowel syndrome is a chronic functional gastrointestinal disorder characterized by abdominal pain/discomfort and altered bowel habits. The use of Lactobacilli as probiotics during irritable bowel syndrome is based on their interesting mechanisms of action and their excellent safety profile but little is known about their clinical efficacy due to the lack of adequately designed clinical trials. The current clinical trial protocol aims to determine the effects of a mixture of Lactobacillus acidophilus NCFM an...
Source: Cellular and Molecular Biology - October 6, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Null genotype of GSTT1 may be associated with esophageal cancer risk: a meta-analysis in Chinese individuals.
This study suggested that GSTT1 null genotype may be potential biomarkers for esophageal cancer in China, especially in North China. Studies with larger sample sizes and wider spectrum of populations are warranted to verify this finding. PMID: 28980936 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - October 6, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

MiR-21 functions oppositely in proliferation and differentiation of neural stem/precursor cells via regulating AKT and GSK-3 β.
In this study, we demonstrated that miR-21 reduced proliferation and promoted neural differentiation in NSPCs via regulating the activation of AKT and GSK-3β signaling pathways in vitro. During differentiation of NSPCs, the expression of miR-21 was increased in a time-dependent manner by qRT-PCR. Synthesized pre-miR-21 or anti-miR-21 was transfected into NSPCs, thereby efficiently overexpressing or knocking down miR-21. Overexpression of miR-21 promoted the neural differentiation of NSPCs, as indicated by Tuj1 and PSA-NCAM staining. Interestingly, knocking down miR-21 had the opposite effect of neural differentiation ...
Source: Cellular and Molecular Biology - October 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effects of alpha lipoic acid on acrylamide-induced hepatotoxicity in rats.
This study was carried out to study the protective effect of alpha lipoic acid on acrylamide induced perturbations in rat liver.  Four groups of rats were studied viz., control rats, acrylamide treated rats, alpha lipoic acid treated rats, and alpha lipoic acid plus acrylamide treated rats. ACR and ALA treatment alone and together caused a signifi-cant increase in hepatic reduced glutathione content while a decrease in hepatic ascorbic content was observed when compared to control group.  ALA pretreatment of acrylamide exposed rats caused no a signifi-cant alteration in superoxide dismutase activity but resulted ...
Source: Cellular and Molecular Biology - October 3, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Evaluation of the nutrient profile of Trachyspermum ammi L. seed under the influence of nanoparticles during germination.
This study aimed to provide new insight of the potential growth promoting effects of the nanoparticles () on plant system. Different concentrations of two nanoparticles, that is, iron pyrite (FeS2) and molybdenum disulphide (MoS2) at three different concentrations of 25ug/ml, 50ug/ml and 75ug/ml were tested on the seeds of Trachyspermum ammi L. The data indicated that nanoparticles enhanced the seedling growth as greener leafs and increased lengths of epicotyl and hypocotyls were seen. These nanoparticles also showed the potential to increase the contents of primary metabolites during germination and the total soluble prot...
Source: Cellular and Molecular Biology - October 3, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Curcumin mediated attenuation of carbofuran induced toxicity in the heart of Wistar rats.
Authors: Jaiswal SK, Gupta VK, Siddiqi NJ, Sharma B Abstract Carbofuran is used to improve the agricultural productivity as well as to protect the house hold and industrial products, but due to accumulation in the biological system, it causes serious side effects in many non-targets mammalian systems. The aim of present study is to evaluate the carbofuran induced oxidative stress in rat heart and its attenuation by using herbal product curcumin. Rats were divided into four groups; one group received 20 % LD50 of carbofuran another group of rats received same doses of carbofuran was  pretreated with curcumin (1...
Source: Cellular and Molecular Biology - October 3, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Synthesis, characterization of nano-sized anatase TiO2   and its adsorption behaviour  for environmental contaminant.
Synthesis, characterization of nano-sized anatase TiO2  and its adsorption behaviour  for environmental contaminant. Cell Mol Biol (Noisy-le-grand). 2017 Jul 31;63(6):18-20 Authors: Gupta K, Pandey A, Singh RP Abstract TiO2 is one of the most studied material due to its unique properties like chemical stability, non toxicity and cost effectiveness.  Nano-sized TiO2   has been prepared by sol -gel method using titanium butoxide as precursor. X- ray diffraction analysis was used to characterize the phase and crystal size of the sy...
Source: Cellular and Molecular Biology - October 3, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Prevalence of COMT Val158Met polymorphism in Eastern UP population.
Authors: Kumar P, Yadav U, Rai V Abstract Catechol-O-methyltransferase (COMT) is an abundant S-adenosylmethionine (SAM-)-dependent methyltransferase that methylates catechol compounds, including catecholamines and catecholestrogens.COMT  gene located at chromosome 22q11.2 contains a functional polymorphism at codon 158(Val158Met), which has been related to psychiatric diseases and different types of cancer. COMT might affect tHcy levels because as a by-product it converts SAM to S-adenosylhomocysteine (SAH), which is reversibly converted to homocysteine. The aim of the present study was to determine the freque...
Source: Cellular and Molecular Biology - October 3, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effect of immobilisation on production of rapamycin by Streptomyces hygroscopicus.
This study is another attempt in this direction which involves immobilisation of producer organism on various supports such as polyutherane foam, sintered beads, glass beads and foam peanuts. These support materials have been selected based on their availability and were pre-treated chemically to improve their adsorption capacity. Comparative profiles have been studied to identify the most suited support for the growth of Streptomyces sp. Variation of biomass immobilisation on various support materials with respect to time in batch culture was studied. Cell release study for the carriers was carried out in production mediu...
Source: Cellular and Molecular Biology - October 3, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Abstraction of phenol onto Pseudomonas putida and Cetyl Trimethyl Ammonium Bromide.
Authors: Saravani N, Arulmozhi M, Anbuthangam A, Manimozhi M Abstract Foam separation, an efficient downstream processing unit operation, was tested as a post-treatment technique for phenol removal after biosorption. The biosorptive foam separation process was carried out in two stages, namely biosorption and foam separation. A minimum run resolution V central composite design with four variables (initial concentration, pH, biosorbent dosage, and time) for biosorption and three variables (liquid pool height, surfactant concentration, and air flow rate) for biosorptive foam separation was applied to optimize the pro...
Source: Cellular and Molecular Biology - October 3, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effects of arsenic toxicity on morphological characters in blackgram (Vigna mungo L.) during early growth stage.
This study indicates that arsenic toxicity causes the deleterious effect on blackgram growth. The toxic effect of blackgram depends on the genotypic variability. Some blackgram genotypes show very less toxic effect of arsenic due to its genetic makeup. Experimental findings of study indicate that longer root length and more shoot weight in arsenic stress condition may be tolerant blackgram genotype to arsenic toxicity. PMID: 28968207 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - October 3, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Efficacy and Stability studies of microbial folate fortified fruit juices prepared using probiotic microorganism.
Authors: Deep S, Ojha S, Kundu S Abstract Folate, natural form of water soluble vitamin folic acid, is significant for humans as involved in most important metabolic reactions i.e. nucleotide synthesis and amino acid inter conversions. Thus its deficiency causes neural tube defects in newborns and cardiovascular diseases, and cancers. Humans cannot synthesize folate de novo so consumption through diet is essential. Natural food sources, supplements and fortified food products are the choices available to complete the Daily recommended intake. However microbial fortification using probiotics recently gained wide att...
Source: Cellular and Molecular Biology - October 3, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Comparative Study of Rhodamine B degradation by Tio2 nanoparticles synthesized from titanium sec butoxide and its chloroacetato derivatves.
Authors: Ambreen S, Pandey ND, Pandey A Abstract TiO2 has been well recognized as a proficient photocatalyst. TiO2 nanoparticles have been synthesized from titanium sec butoxide (1) and its monochloroacetate derived compounds. The modifications of Ti(OsBu)4 with monochloroacetic acid in 1:1 and 1:2 molar ratios afforded Ti(OsBu)3(OOCCH2Cl) (2) and Ti(OsBu)2(OOCCH2Cl)2 (3), respectively. The use of monochloroacetic acid as a modifier allows the control of both the degree of condensation and oligomerization of the precursor. The cross linking of the gel and connectivity of the molecular building blocks are lowered in...
Source: Cellular and Molecular Biology - October 3, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Plant derived antimicrobial peptide Ib-AMP1 as a potential alternative drug candidate for Staphylococcus aureus toxins.
Authors: Ojha S, Deep S, Kundu S Abstract Due to an increase in the occurrence of multi drug resistant microorganisms a need for the development of alternative drugs comes in light. This alternative drug should be such that the microorganisms should not be able to develop resistance against them easily. Antimicrobial peptides are the most potential candidates to be developed as alternative drug. In the present study the three toxins ETA, ETB and PVL of Staphylococcus aureus were docked with four antimicrobial peptides, Ib-AMP1, JCPep7, Snakin2, Sesquin, derived from plants. The docking studies predict that Ib-AMP1 ...
Source: Cellular and Molecular Biology - October 3, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

H2O2 sensing through electrochemically deposited thionine coated ITO thin film.
Authors: Singh P, Srivastava S, Singh SK Abstract Progression and initiation of different diseases including pulmonary diseases, alzheimer's and tumors are linked with the oxidative stress, an important cause of cell damage. Different antioxidant enzymes are involved in detoxifying reactive oxygen species including hydrogen peroxide (H2O2) that is generated in response to various stimuli and has important role in cell activation & bio-signaling processes. Herein, we developed hydrogen peroxide electrochemical sensor based on horseradish peroxidase (HRP) entrapped polymerized thionine (PTH) film. Electrochem...
Source: Cellular and Molecular Biology - October 3, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effect of pH on optimization of photofermentative hydrogen production by co-culture of Rhodobacter sphaeroides-NMBL-02 and Bacillus firmus-NMBL-03.
Authors: Pandey A, Dolly S, Semwal D, Pandey A Abstract Rhodobacter sphaeroides NMBL-02, photosynthetic purple non sulfur (PNS) bacteria and associated Bacillus firmus NMBL-03 were isolated from water sample collected from 15-20 inches beneath the surface of ponds from Northern region of India in modified Sistrom's media (120 ml) containing 3 g/L malate and 1.2 g/L ammonium sulfate. The isolation was done in air tight serum bottles (120 ml) under tungsten bulb (1.8 kLux light intensity) at 30 oC ± 2 oC. The PNS and heterotrophic bacteria associated with the culture was purified by clonal selection method and...
Source: Cellular and Molecular Biology - October 3, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Pharmacological activities of cinnamaldehyde and eugenol: antioxidant, cytotoxic and anti-leishmanial studies.
Authors: Sharma UK, Sharma AK, Gupta A, Kumar R, Pandey A, Pandey AK Abstract The present study reports the antioxidant, cytotoxic and anti-leishmanial activities of cinnamaldehyde and eugenol. Both the compounds are naturally present in cinnamon and bay leaf. Eugenol is abundantly present in clove. The antioxidant potential was measured in terms of reducing power (FRAP assay), nitric oxide (NO) radical scavenging ability and anti-lipid peroxidative activities. MTT assay was used to evaluate the effect of test compounds on cell viability of prostate cancer cell line (PC-3) as well as for the assessment of in vitro ...
Source: Cellular and Molecular Biology - October 3, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effect of Microbial inoculation in combating the aluminium toxicity effect on growth of Zea mays.
Authors: Arora P, Singh G, Tiwari A Abstract The present study is aimed at improving the aluminium tolerance in maize crop employing the potential of microbial inoculants in conferring resistance to these toxicities via production of certain chelating compounds like siderophores, exopolysachharides and organic acids. Acid soils have now-a-days become one of the key factors for limiting growth of many agriculturally important crops. Aluminium  is one of the major elements present in acid soils and is mainly responsible for toxicity in the soil. This aluminium is rapidly soluble in soil water and hence absorbed ...
Source: Cellular and Molecular Biology - October 3, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Mathematical modelling of bioethanol production from algal starch hydrolysate by Saccharomyces cerevisiae.
Authors: Singh S, Chakravarty I, Kundu S Abstract Bioethanol is an excellent alternative for petrol and has long-term economic advantages over non-renewable liquid biofuels. Bioethanol can be produced from different biomass materials, and it is categorized into three generations by biomass. First generation bioethanol directly competes with food items while second generation bioethanol requires more land area and fertilizers. Bioethanol produced from algae comes under third generation bioethanol and has many advantages over first and second generation bioethanol. Algae have higher growth rates than plants and requi...
Source: Cellular and Molecular Biology - October 3, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Exploitation of dark fermented effluent of cheese whey by co-culture of Rhodobacter sphaeroides and Bacillus firmus for photo-hydrogen production.
In this study photo-hydrogen production from cheese whey dark fermentation (DF) effluent by the co-culture of Rhodobacter sphaeroides -NMBL-01 and Bacillus firmus - NMBL-03 has been reported. The effect of pH, initial chemical oxygen demand (COD) and the concentration effect of FeSO4.7H2O on photo-hydrogen production have been investigated. The end products of dark fermentation effluent of cheese whey were mainly comprised of soluble organic acids, i.e. butyric acid and lactic acid. The batch process was carried out under light intensity of 2.5 kLux at 32 ± 2oC without any addition of extra carbon and nitrogen sourc...
Source: Cellular and Molecular Biology - October 3, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Lung cancer: analysis of biomarkers and methods of diagnostic and prognostic value.
Authors: Yadav S, Agrawal S, Divya Ravali SS, Pandey A Abstract This paper summarizes the overwhelming evidence that targeted therapy is better than chemotherapy for the treatment of lung cancer. The focus is on lung cancer because of the sheer magnitude of this disease especially in males. In India, lung cancer constitutes 6.9 per cent of all new cancer cases and 9.3 per cent of all cancer related deaths in both sexes (1). Currently the world we reside has a norm of developing cure to an abnormal state of living body (so called disease) based on evidences, recognized during the diagnosis of the disease. It is done...
Source: Cellular and Molecular Biology - October 3, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Association of C677T polymorphism (rs1801133) in MTHFR gene with depression.
Authors: Rai V Abstract Depression is one of the mental disorders with a state of low mood and aversion to activities that exerts a negative effect on a person's thoughts and behavior. Genetic association studies on MTHFR C677T polymorphism and depression have been repeatedly performed over the last two decades, but results are inconsistent. The aim of the present study was to assess the relationship between MTHFR C677T polymorphism and depression by literature review and meta-analysis. Four electronic databases, PubMed, Google Scholar, Science direct and Springer Link were searched for case control articles focusi...
Source: Cellular and Molecular Biology - October 3, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

LncRNA H19 contributes to Rh2-mediated MC3T3-E1cell proliferation by regulation of osteopontin.
In this study, we assessed the expression of 17 long non-coding RNAs (lncRNAs) in cultured MC3T3-E1 cells under Rh2 treatments. We found that lncRNA H19 was significantly increased in Rh2-treated MC3T3-E1 cells. Expression of lncRNA H19 was promoted in a dose- and time-dependent manner after Rh2 treatments. Increased expression of lncRNA H19 resulted in osteopontin (OPN) overexpression in Rh2-treated MC3T3-E1 cells. Furthermore, knockdown of lncRNA H19 by specific siRNAs significantly abolished the Rh2-mediated cell proliferation effects. Knockdown of lncRNA H19 also decreased both mRNA and protein levels of OPN in the Rh2...
Source: Cellular and Molecular Biology - September 9, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Pleckstrin homology-like domain family B member 1 rs498872 polymorphism and glioma risk in Chinese Han population.
In conclusion, this study found that PHLDB1 rs498872 polymorphism was significantly associated with glioma risk in Chinese Han population. PMID: 28886307 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - September 9, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Contribution of polymorphisms in the LEP, LEPR and RETN genes on serum leptin and resistin levels in young adults from Mexico.
Authors: López-Quintero A, García-Zapién AG, Flores-Martínez SE, Díaz-Burke Y, González-Sandoval CE, Lopez-Roa RI, Medina-Díaz E, Muñoz-Almaguer ML, Sánchez-Corona J Abstract Polymorphisms in the LEP (G-2548A and A19G), LEPR (A326G, A668G and G3057A) and RETN (C-420G and G+62A) genes were documented according to their association with alterations in biochemical parameters such as glucose, insulin and lipid profiles, along with serum leptin and resistin concentrations. The aim of the study was to establish any contribution of the G-2548A and A19G polymor...
Source: Cellular and Molecular Biology - September 9, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The correlation between Pax5 deletion and patients survival in Iranian children with precursor B-cell acute lymphocytic leukemia.
Authors: Moafi A, Zojaji A, Salehi R, Najafi Dorcheh S, Rahgozar S Abstract Despite advances in treatment, children with acute lymphoblastic leukemia (ALL) still experience drug resistance and relapse. Several gene mutations are involved in the onset of this disease and resistance to therapy. The present study examines the incidence of IKZF1, CDKN2A/B, PAX5, EBF1, ETV6, BTG1, RB1, JAK2, and Xp22.33 gene deletions/duplications associated with pediatric ALL in Iran and investigates the possible effect of these mutations on drug resistance. Three-year disease-free survival (3DFS) was evaluated for children diagnosed w...
Source: Cellular and Molecular Biology - September 9, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Risk factors associated with oral and maxillofacial benign tumors: A case-control study.
This study aimed to investigate the risk factors for oral and maxillofacial benign tumors (OMFBTs). A total of 138 patients diagnosed with OMFBTs between September 2010 and September 2015 were retrospectively analyzed. Clinical data including demographic characteristics, smoking and drinking status, dietary habit, oral hygiene and tumor related family history were collected and compared with 134 cases of healthy people who visited the hospital for physical examination during the same time. Logistic regression analysis was performed for multivariable regression analysis. OMFBTs was associated with smoking more than 20 cigar...
Source: Cellular and Molecular Biology - September 9, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effect  of sucrose concentrations on Stevia rebaudiana Bertoni tissue culture and gene expression.
Effect of sucrose concentrations on Stevia rebaudiana Bertoni tissue culture and gene expression. Cell Mol Biol (Noisy-le-grand). 2017 Aug 30;63(8):33-37 Authors: Ghorbani T, Kahrizi D, Saeidi M, Arji I Abstract Stevia rebaudiana (Bert.) Bertoni is known as sweet plant which it contains a high level of steviol glycosides in the leaves.  This plant has been used from centuries ago as a sweetener for tea. One of the most important steviol glycosides is stevioside that is attractive for diabetic persons. Tissue culture is the only rapid process for the mass propagation of stevia. One of the...
Source: Cellular and Molecular Biology - September 9, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Differences in expression of serum protein in patients with psoriasis vulgaris and blood heat syndrome and healthy volunteers.
Authors: Hao P, Yang C, Yan X, Li T, Zhang J, Yu Q, Zhang X, Zhang Y Abstract To explore the mechanism of psoriasis vulgaris (PV), serum protein expression profiles between PV patients with blood-heat syndrome and healthy volunteers were detected by isobaric tags for relative and absolute quantitation (iTRAQ). First, sera from 15 PV patients with blood-heat syndrome and 10 healthy volunteers were collected; then, serum proteins were separated and hydrolyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a specific iTRAQ marker enzyme respectively after further purification and protein a...
Source: Cellular and Molecular Biology - September 9, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Susceptibility of herpes simplex virus type 1 to monoterpenes thymol, carvacrol, p-cymene and essential oils of Sinapis arvensis L., Lallemantia royleana Benth. and Pulicaria vulgaris Gaertn.
CONCLUSION: These results indicate that compounds affected HSV-1 mostly before adsorption and might interact with the viral envelope. Thymol exhibited a high selectivity index and seems to be a promising candidate for topical therapeutic application as antiviral agent for treatment of herpetic infections. PMID: 28886313 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - September 9, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Alterations on high HbF levels may be associated with KLF1 gene mutations.
In this study, in order to determine the relationship between the KLF1 mutations and the high HbF levels three exons along with the 5'-UTR and 3'-UTR regions of the KLF1 gene were sequenced of 53 volunteers. In this study, 3 variations in the non-coding regions of the KLF1 gene were not associated with a high level of HbF. Five variations were detected in the second exon of KLF1 gene. One of these is a frame shift that occurs when GG bases are inserted between the 59-60 codons, and the other four variations occur as a base substitution variations.  No correlation was found between high HbF levels and neutral variants....
Source: Cellular and Molecular Biology - September 9, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The protective effect of cinnamaldehyde on  lipopolysaccharide induced acute lung injury in mice.
The protective effect of cinnamaldehyde on lipopolysaccharide induced acute lung injury in mice. Cell Mol Biol (Noisy-le-grand). 2017 Aug 30;63(8):58-63 Authors: Huang H, Wang Y Abstract The study wasintended to examinethe protective effect of cinnamaldehyde (CM) on lipopolysaccharide (LPS)-induced acute lung injury (ALI) mice model.The results of the investigation confirmed that, LPS induced inflammatory cytokines such as TNF-α, IL-6, IL-13 and IL-1β were significantly decreased by CM. CM also up-regulated level of IL-10. We found that CM significantly attenuated LPS-induced TLR4 expre...
Source: Cellular and Molecular Biology - September 9, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Is GDF5 gene promoter polymorphism +104T/C associated with osteoarthritis in the Eastern of Turkey population?
Authors: Tülüce Y, Yildirim IH, Özkol H, Edi Z L, Delen V Abstract Osteoarthritis (OA) is the most common form of arthritis. Genetic factors have been shown to play important roles in the etiology of OA. The gene growth differentiation factor 5 (GDF5) has been implicated in skeletal development and joint morphogenesis in human and mice. A functional single nucleotide polymorphism (SNP) +104T/C in the 5'-UTR of GDF5 (rs143383) was reported to be associated with osteoarthritis susceptibility in Han Chinese and Japanese populations. Our objective was to assess whether this SNP was also associated with O...
Source: Cellular and Molecular Biology - September 9, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Potential of Laurencia obtusa as a substrate for the development of a probiotic Saccharomyces cerevisiae.
In conclusion, it was determined that the L. obtusa extract added to the development medium of S. cerevisiae caused differences in the synthesis of some vitamins and fatty acids. PMID: 28886317 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - September 9, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

New insights into the expression profile of MicroRNA-34c and P53 in infertile men spermatozoa and testicular tissue.
In this study, we investigate the role of miRNAs in infertile males.The patients were assigned to five groups based on semen analysis (n=55), including normozoospermic (N), moderate oligoasthenoteratozoospermic (MOAT), severe oligoasthenoteratozoospermic (SOAT), obstructive azoospermia (OA) and non-obstructive azoospermia (NOA). Quantitative RT-PCR was recruited to study the expression of miR-34c and tumor suppressor p53 gene. In addition, malondialdehyde (MDA) and DNA fragmentation was measured. Network analysis was performed using Pathway Studio web tool (Elsevier). Our results revealed statistically significan...
Source: Cellular and Molecular Biology - September 9, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Morphokinetic analysis of human embryo development and its relationship to the female age: a retrospective time-lapse imaging study.
Authors: Akhter N, Shahab M Abstract Time-lapse imaging technique has provided embryologists with a unique chance of studying the embryo morphokineticsto select the most viable embryos for implantation in the field of IVF (In vitro fertilization).The conventional method of morphological evaluations has proved that maternal age affects the human embryo quality. This retrospective study carried out at Islamabad Clinic Serving Infertile Couples, Islamabad, Pakistan mainly focuses on the effect of female age on human embryo morphokinetics. A total number of 200 patients undergoing ICSI treatment cycles at the clinic we...
Source: Cellular and Molecular Biology - September 9, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Investigation of the irisin, preptin  and adropin levels in the blood serum of Alburnus tarichi.
This study used 12 adult A. tarichi (6 female and 6 male) obtained from Van Lake (Van, Turkey). The serum irisin, preptin and adropin levels were measured using a commercially available enzyme-linked immunosorbent assay (ELISA) kit to determine correlations between the levels of these three hormones and fish body weight and length.  No statistically significant correlations were detected between the serum irisin, adropin and preptin levels and A. tarichi body weight (p = 0.921, r = -0.031; p = 0.08, r = 0.519; p = 0.461, r = -0.235, respectively) or length (p = 0.901, r = -0.040; p = 0.105, r = 0.490; p = 0.236, r = -...
Source: Cellular and Molecular Biology - September 9, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Relationship between MCP1 (-2518A > G) gene variants and ovarian cancer in Turkish population.
This study suggests that MCP-1 (-2518A>G) AG genotype and G allele could be considered as risk factor for susceptibility to ovarian cancer. PMID: 28886321 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - September 9, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Carnosol inhibits Hedgehog signaling pathway in both LNCaP and DU145 prostate cancer cell lines.
Authors: Gao H, Song Q, Yang J, Yu S, Zhao J, Yu G Abstract To investigate the effect of carnosol on the Hedgehog (HH) signaling pathway in human hormone-dependent prostate cancer cell line LNCaP and hormone-independent prostate cancer cell line DU145. The expression levels of glioma-associated oncogene homolog 1 (Gli1) and Sonic hedgehog (Shh) in human prostate cancer tissues were detected by immunohistochemistry. After treated with carnosol (0.25-16 μmol/L), the cell survival of LNCaP and DU145 cells were detected by MTT assay. The expression levels of Gli1 and Shh mRNA and protein in the two cells were detect...
Source: Cellular and Molecular Biology - September 9, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Genotoxic and cytotoxic potential of Alternanthera Bettzickiana, an important ethno-medicinal plant.
Authors: Akhtar MF, Sharif A, Saleem M, Saleem A, Akhtar B, Raza M, Ijaz H, Shabbir M, Ali S, Sharif A, Nasim MB, Peerzada S Abstract The present study was carried out to investigate the mutagenic and cytotoxic potential of n-hexane and aqueous-methanolic whole plant extracts of Alternanthera bettzickiana. Aqueous-methanolic and n-hexane extracts of Alternanthera bettzickiana extracts were assessed for the mutagenic potential with Salmonella tester strains TA-100 and TA-102 in the presence and absence of the rodent enzyme activation system and cytotoxic potential was assessed by MTT assay. Aqueous-methanolic extrac...
Source: Cellular and Molecular Biology - September 9, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Atypical bacterial etiology of acute respiratory infections and clinical characterizations among Iranian children.
Authors: Halaji M, Hashempour T, Pouladfar GR, Ghasabi F, Khashei R Abstract Acute respiratory infections (ARIs) in children younger than 5 years of age are one of the leading causes of morbidity and mortality, particularly in developing countries. Mycoplasma pneumoniae and Chlamydophila pneumoniae are prevalent causative agents of ARIs, worldwide. We sought M. pneumoniae and C. pneumoniae in respiratory samples from Iranian children with ARIs.  From November 2014 to April 2015, respiratory samples of 150 children aged 1 month to 15 years old were screened for presence of M. pneumoniae and C. pneumoniae. Polym...
Source: Cellular and Molecular Biology - September 9, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Nullification of aspirin induced gastrotoxicity and hepatotoxicity by prior administration of wheat germ oil in Mus musculus: histopathological, ultrastructural and molecular studies.
Authors: Mohamed HRH, Hamad SR Abstract Aspirin (salicylic acetyl acid) is used worldwide to treat various inflammatory conditions and prevent cardiovascular disease, along with reducing the risk of cancer. However, administration of aspirin causes toxic effects, especially in the stomach and liver. Thus, our study examined the protective effect of wheat germ oil on aspirin-induced toxicity in the stomach and liver tissues of albino white mice. Administration of wheat germ oil before aspirin has restored normal hepatic and gastric tissue architecture and DNA integrity has become better than that of a negative healt...
Source: Cellular and Molecular Biology - September 9, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

miR-146a rs2910164 polymorphism might be associated with coronary artery disease risk in Asians.
In conclusion, this meta-analysis found that miR-146a rs2910164 polymorphism was significantly associated with CAD risk. PMID: 28886326 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - September 9, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research