Virulence genes in biofilm producer Enterococcus faecalis isolates from root canal infections.
This study aimed to evaluate the presence of E. faecalis virulence genes in dental root canal isolates recovered from advanced chronic periodontitis patients. One hundred E. faecalis isolated from dental root canal during July 2015 to Oct 2016. After analysis of biofilm formation by the semi-quantitative determination in 96-well flat bottom polystyrene plates, the presence of asa, esp, efaA, ace, ebpR, gel and hyl gene were studied by PCR. Gelatinase and hemolytic activity were detected by phenotypic methods. Ninety-one percent of isolates had ebpR gene, 85% ace, 82% efaA, 81% gel, 56% esp, 33% asa1, 2% hyl and 0% cyl gene...
Source: Cellular and Molecular Biology - July 20, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Regulatory effects of the novel synthesized Indole-3-carbaldehyde on expression of cell cycle genes: A study on Cyclin D and P21 expression by acute promylocytic leukemia cell line (NB4).
Authors: Karimabad MN, Mahmoodi M, Jafarzadeh A, Darekordi A, Hajizadeh MR, Khorramdelazad H, Sayadi AR, Khanamani Falahati-Pour S, Hassanshahi G Abstract A large variety of heterocycles are known to date and among these, indole and pyran rings are of particular interest. The present investigation was aimed to investigate the effects of novel Indole-3-carbaldehyde derivative (NI-3-CD) analogue on growth inhibition of cells and its regulatory effects on expression of cell cycle genes in acute promylocytic leukemia (APL) cell line. NB4 cells were cultured in presence of RPMI1640 medium contained various concentration...
Source: Cellular and Molecular Biology - July 20, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

TRIM59 is a key regulator of growth and migration inrenal cell carcinoma.
In this study, we aimed to investigate the role of TRIM59 in the cell growth and migration in RCC. The expression of TRIM59 in human RCC tissues was initially examined by qRT-PCR. Alentivirus-based shRNA against TRIM59 (Lv-shTRIM59) was constructed. The effects of TRIM59 knockdown on cell proliferation were examined by in vitro MTT assay, colony formation assay and in vivo a mouse xenograft model of RCC. Cell migration and invasion after knockdown of TRIM59 were also examined by transwell assay. Our data showed that the mRNA level of TRIM59 in cancerous tissues was 2-fold increased as compared with non-cancerous tissues. K...
Source: Cellular and Molecular Biology - July 20, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Identification of G2607A mutation in EGFR gene with a significative rate in Moroccan patients with Bladder Cancer.
In this study, we evaluated the frequency of point mutations in EGFR for future use of TKI in clinical treatment of bladder cancer. A total, 50 Moroccan patient specimens with bladder cancer and 48 healthy controls were analysed for EGFR mutations in the region delimiting exons 18-21 by PCR amplification and direct sequencing. Our results showed the absence of mutations in the EGFR kinase domain in these exons in all analysed specimens. However, sequence analysis of the EGFR-TK domain, revealed the presence of (G2607A) polymorphism at exon 20. Statistical analysis showed significant difference in the frequencies of G2607A ...
Source: Cellular and Molecular Biology - July 20, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Characterization of Candidate probionts isolated from human breast milk.
This study was designed to isolate and identify the potential probionts present in 32 healthy mothers' breast milk. Microbial culture media and 16SrRNA sequencing were used to isolate and identify the bacteria and all isolates were analyzed for their antagonistic potential, resistance to acidic pH, bile salts and survival under simulated gastric and intestinal conditions. The colonization potential was further assessed based on adherence to human enterocyte-like Caco-2 cell lines. The breast milk samples harbored significant numbers of Gram positive and catalase negative (85%) bacteria. Based on 16SrRNA sequencing, these i...
Source: Cellular and Molecular Biology - July 20, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

CCAT1 expressed in malignant and pre-malignant human gastric tissues.
Authors: Li X, Zhou Y, Qian H Abstract CCAT1 has been reported to implicate in various human malignancies. However, its expression in the oncogenesis of gastric cancer (GC) remains unknown. Quantitative reverse transcription (RT)-qPCR assay was performed to detect the expression of CCAT1 in 68cases of GC tissues,paired tumor-proximal gastric mucosa and normal gastric mucosa, 19 cases of gastric intraepithelial neoplasia (GIN), and the sera of these 68 GC patients and 22 healthy volunteers. And the relationship between CCAT1 and clinicopathologic features of GC patients were analyzed. Inthe present study, we found t...
Source: Cellular and Molecular Biology - July 20, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Determination of IL-6, TNF- α and VEGF levels in the serums of patients with colorectal cancer.
In this study, no significant difference was obtained between the mean scores concerning the IL-6 and VEGF serums of the colorectal cancer and healthy group (p>.05). But a statistically significant decrease was observed in the TNF-α serum level of the colorectal cancer group in comparison with the control group (p= .016; p < .05). PMID: 28719352 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - July 20, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effect of Triptolide on retinal ganglion cell survival in an optic nerve crush model.
In this study, we investigated the changes in retinal ganglion cell survival and axonal regeneration after administration of triptolide in optic nerve crush model. Triptolide treatment tended to promote retinal ganglion cell survival rather than optic nerve regeneration as well as inhibit the expression of tumor necrosis factor-α and activation of nuclear factor-kappa B. These findings suggested that intraperitoneal injection of triptolide may be an effective treatment for optic nerve injury and this effect was attributed at least in part to its anti-inflammatory actions. PMID: 28719353 [PubMed - in process] (Sou...
Source: Cellular and Molecular Biology - July 20, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Thymol and carvacrol strongly inhibit biofilm formation and growth of carbapenemase-producing Gram negative bacilli.
Authors: Raei P, Pourlak T, Memar MY, Alizadeh N, Aghamali M, Zeinalzadeh E, Asgharzadeh M, Kafil HS Abstract Discovery of novel drugs with new mechanisms of action and without cross-reaction with current therapeutic agents is crucial in the management of infections caused by multi-drug resistant (MDR) bacteria. The aim of the present study was to investigate effects of carvacrol and thymol on biofilm formation and antimicrobial activity against different carbapenemase-producing Gram negative bacilli. The antimicrobial and antibiofilm effect of thymol and carvacrol was investigated against strains harboring differe...
Source: Cellular and Molecular Biology - July 20, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

miRNA-223 suppresses FOXO1 and functions as a potential tumor marker in breast cancer.
In conclusion, we demonstrated that the miRNA-223can maintain cell proliferation of breast cancer cell through targeting FOXO 1, these results provide a new insight in tumor marker and potential therapeutic targets for breast cancer. PMID: 28719355 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - July 20, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Co-incubation of PMN and CaCo-2 cells modulates inflammatory potential.
We examined interactions of PMN and intestinal epithelial cell-like CaCo-2 cells to elucidate their regulation of inflammatory signalling and the impact of cyclooxygenase (COX), nitric oxide (NO) and platelet-activating factor (PAF). Human PMN and CaCo-2 cells, separately and in co-incubation, were stimulated with the calcium ionophore A23187 or with N-Formyl-methionyl-leucyl-phenylalanin (fMLP) that activates PMN only. Human neutrophil elastase (HNE) and respiratory Burst were measured. To evaluate the modulation of inflammatory crosstalk we applied inhibitors of COX (acetyl salicylic acid; ASA), NO-synthase (N-monomethyl...
Source: Cellular and Molecular Biology - July 20, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Vitamin D receptor BsmI polymorphism may be associated with an decreased osteoporosis risk in South China.
Authors: An M, Song XB, Chen XY Abstract Although many epidemiology studies have evaluated the correlation between vitamin D receptor (VDR) BsmI polymorphism and osteoporosis, the current results remain inconclusive. This meta-analysis updated and reevaluated the possible association between VDR BsmI polymorphism and osteoporosis in Chinese populations. Studies were identified using PubMed and Chinese databases through December 2016. The associations were assessed with pooled odds ratios (ORs) and 95% confidence intervals (CIs). A total of nine studies with 688 osteoporosis cases and 730 controls were included in t...
Source: Cellular and Molecular Biology - July 20, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The association between the UBQLN1 polymorphism and Alzheimer's disease risk: A systematic review.
Authors: Li X, Zhou J, Chen H, Wang F, Mei Q, Sun H Abstract Recently, some studies suggested that UBQLN1 variant was associated with AD risk. However, the results were inconsistent. This meta-analysis aimed to determine the association between UBQLN1 variant and AD risk. We searched the electronic databases PubMed, Embase, and CNKI databases. Random-effects model was used. All statistical tests were performed using the STATA 11.0 software (StataCorp, College Station, TX, USA). UBQLN1 variant was not associated with the risk of AD (OR=1.05; 95%CI, 0.92-1.19; I2=35%). The corresponding pooled ORs were not materially...
Source: Cellular and Molecular Biology - July 20, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Cell biology, biophysics, and mechanobiology: From the basics to Clinics.
Authors: Zeng Y Abstract Cell biology, biomechanics and biophysics are the key subjects that guide our understanding in diverse areas of tissue growth, development, remodeling and homeostasis. Novel discoveries such as molecular mechanism, and mechanobiological mechanism in cell biology, biomechanics and biophysics play essential roles in our understanding of the pathogenesis of various human diseases, as well as in designing the treatment of these diseases. In addition, studies in these areas will also facilitate early diagnostics of human diseases, such as cardiovascular diseases and cancer. In this special issue...
Source: Cellular and Molecular Biology - May 11, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Platelet-derived miR-142-3p induces apoptosis of endothelial cells in hypertension.
Authors: Bao H, Yao QP, Huang K, Chen XH, Han Y, Jiang ZL, Gao LZ, Qi YX Abstract The dysfunction of endothelial cells (ECs) plays crucial roles in vascular remodeling during hypertension. Researches suggested that ECs are regulated by the circulating platelets in vivo, which may participate in abnormal EC apoptosis in hypertension. However the molecular mechanism in this process is still unclear. Here we focused on the microRNAs (miRs) in platelets, and detected the potential role and delivery mechanism of platelet-derived miRs in ECs. Using microarray, the differentially expressed profile of miRs between platelet...
Source: Cellular and Molecular Biology - May 11, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effect of matrix rigidity on organ-specific capture of tumor cells by flow.
Authors: Ma P, Shen X, Tan P, Yang L, Liu W Abstract Accumulating evidences have suggested that tumor metastasis exists prominent organ discrepancy. In this progression, the capture of intravascular tumor cells (TCs) to endothelium in distant tissues and organs plays a decisive role in the organ-specific metastasis formation. However, the mechanism of tumor cells preferentially arrest and adhere to endothelial cells (ECs) of target organ still remains elusive. By using the parallel plate flow chamber and the polyacrylamide gels with different matrix stiffness, we here explored the combined effects of matrix ri...
Source: Cellular and Molecular Biology - May 11, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Sphingosine-1-phosphate reduces adhesion of malignant mammary tumor cells MDA-MB-231 to microvessel walls by protecting endothelial surface glycocalyx.
Authors: Zhang L, Zeng M, Fu BM Abstract Sphingosine-1-phosphate (S1P) is a sphingolipid in plasma that plays a critical role in cardiovascular and immune systems. Endothelial surface glycocalyx (ESG) decorating the inner wall of blood vessels is a regulator of multiple vascular functions. To test the hypothesis that S1P can reduce tumor cell adhesion to microvessel walls by protecting the ESG, we quantified the ESG and MDA-MB-231 tumor cell adhesion in the presence and absence of 1μM S1P, and in the presence of the matrix metalloproteinase (MMP) inhibitor in post-capillary venules of rat mesentery. We also meas...
Source: Cellular and Molecular Biology - May 11, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Role of microRNA-124 in cardiomyocyte hypertrophy inducedby angiotensin II.
In conclusion, our study shows that inhibition of miR-124 effectively suppresses AngII-induced myocardial hypertrophy, which is associated with attenuation of ER stress. PMID: 28478799 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - May 11, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

HIF-1 α mediates visfatin-induced CTGF expression in vascular endothelial cells.
In conclusion, the up-regulation of CTGF expression by visfatin might be mediated via HIF-1α -dependent pathway, but not the TGF-β1 pathway in EA.Hy926 cells. PMID: 28478800 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - May 11, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Involvement of TRPC1 in Nampt-induced cardiomyocyte hypertrophy through the activation of ER stress.
Authors: Li J, Wu W, Zhao M, Liu X Abstract Nicotinamide phosphoribosyltransferase (Nampt) is involved in the development of cardiac hypertrophy. Transient receptor potential canonical channel 1 (TRPC1) and endoplasmic reticulum stress (ER stress) are regarded as critical pathways in cardiac hypertrophy. Therefore, we hypothesizedthat TRPC1 might be associated with ER stress in Nampt-induced cardiac hypertrophy. CulturedH9c2cardiomyocyteswereexposed to Namptfor different timesand the expression of markers of cardiomyocyte hypertrophy and ER stress, as well as TRPC1 were detected. Moreover, specific TRPC1-shRNA (sho...
Source: Cellular and Molecular Biology - May 11, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Electroacupuncture ameliorates spatial learning and memory impairment via attenuating NOX2-related oxidative stress in a rat model of Alzheimer's disease induced by A β1-42.
Electroacupuncture ameliorates spatial learning and memory impairment via attenuating NOX2-related oxidative stress in a rat model of Alzheimer's disease induced by Aβ1-42. Cell Mol Biol (Noisy-le-grand). 2017 Apr 29;63(4):38-45 Authors: Wu G, Li L, Li HM, Zeng Y, Wu WC Abstract Alzheimer's disease (AD) is a chronic neurodegenerative disorder characterized by progressive deterioration of cognition and memory, in which oxidative stress has been played a crucial role in the pathology of AD. Electroacupuncture (EA) is a widely used therapy based on traditional acupuncture combined with modern electro...
Source: Cellular and Molecular Biology - May 11, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Peptide 11R-VIVIT stimulates osteoblastogenesis through regulating the expression of nuclear factor of activated T cells cytoplasmic 1.
Authors: Li M, Wang X, Bian Z, Yao W, He Q, Tian F, Zhang J, Zhu L Abstract Osteoporosis is characterized by the imbalance of two relatively independent processes-osteoblastogenesis and osteoclastogenesis. Calcineurin (Cn)/nuclear factor of activated T cells (NFAT)(Cn/NFAT) signaling pathway is involved in these two processes in bone metabolism, but its potential as a target to treat osteoporosis needs to be defined. The aim of this study is to investigate the inhibition of polypeptide 11R-VIVIT onCn/NFAT signaling pathway. Rat calvaria (RC) cells were prepared from experimental model of osteoporosis in rat.11R-VIV...
Source: Cellular and Molecular Biology - May 11, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Connexin 43 enhances oxaliplatin cytotoxicity in colorectal cancer cell lines.
This study suggested that connexin 43 could be considered a molecular target of oxaliplatin activity in colorectal cancer. PMID: 28478804 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - May 11, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Clostridium butyricum partially regulates the development of colitis-associated cancer through miR-200c.
Authors: Xiao Y, Dai X, Li K, Gui G, Liu J, Yang H Abstract Colitis-associated cancer (CAC), one form of colorectal cancer (CRC),is an increasing concern worldwide. Both diagnosis and current therapy are challenging and bottlenecked. The aim of this study is to investigate novel mechanisms by which the therapeutic C. butyricum regulates colitis-induced oncogenesis. Mouse models of CAC were established with 2,4,6-Trinitrobenzenesulfonic acid (TNBS)and azoxymethane (AOM), following by biochemical, clinical and histological analysis. The integrity of epitheliumwas examined by electron microscopy (EM). The epithelial b...
Source: Cellular and Molecular Biology - May 11, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The emerging role of oxidative stress in regulating autophagy: applications of cancer therapy.
Authors: Zhang W, Wan X, Liu Z, Xiao L, Huang H, Liu X Abstract Emerging evidences show that autophagy, as a major cellular adaptive degradation mechanism, is involved in tumorigenesis, cell aging, inflammation and neurodegeneration. It has been reported that multiple stresses including nutrient deprivation, pathogen infection, oxidative stress, endoplasmic reticulum (ER) stress and metabolic stress can influence cellular autophagy, leading to distinct cell fate. Although numerous studies have been employed to elucidate the probable issues, the underlying mechanism of the initiation and maturation of autophagy rema...
Source: Cellular and Molecular Biology - May 11, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Evidence supporting neuroprotective effect of adipose derived stem cells on PC12 cells against oxidative stress induced by H2O2.
Authors: Ghorabi MT, Aliaghaei A, Sadeghi Y, Shaerzadeh F, Rad AA, Mohamadi R, J Ebrahimi M Abstract Adipose-derived stem cells (ADSCs) are a population of cells derived from adipose tissue. ADSCs exhibit multilineage development potential and are able to secrete various factors, which influence adjacent cells. The present study examined the protective effect of ADSC's conditioned media (ADSC-CM) on PC12 cells exposed to H2O2, an oxidative injury model. After isolation, ADSCs were cultured and their osteogenic and adipogenic differentiation confirmed. Then, PC12 cells were co-treated with ADSC-CM and H2O2. Next, th...
Source: Cellular and Molecular Biology - May 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

A proteomics research of apoptosis of leukemia cells induced by arsenic trioxide.
This study could lay the foundation of deciphering mechanisms of HL-60 cell apoptosis and give a new possible target to the clinical application for arsenic trioxide treatment for leukemia. PMID: 28466809 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - May 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Oleanolic acid (OA) regulates inflammation and cellular dedifferentiation of chondrocytes via MAPK signaling pathways.
Authors: Phull AR, Eo SH, Kim SJ Abstract Oleanolic acid (OA) is a bioactive triterpenoid in medicinal plants. It possesses various pharmacological properties, including analgesic, anti-inflammatory, and antitumor effects. The effects of OA in chondrocytes, however, are not well characterized. Here, we used rabbit articular chondrocytes as a cellular model to investigate the effects and regulatory mechanisms of OA on dedifferentiation and pro-inflammation. OA promoted dedifferentiation of chondrocytes by inhibiting type II collagen and pro-inflammatory activity by increasing cyclooxygenase-2 (COX-2) expression. Fur...
Source: Cellular and Molecular Biology - May 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

ARNetMiT R Package: association rules based gene co-expression networks of miRNA targets.
In this study, we introduce ARNetMiT that utilize a hash based association rule algorithm in a novel way to infer the GCNs on miRNA-target genes data. We also present R package of ARNetMiT, which infers and visualizes GCNs of diseases that are selected by users. Our approach assumes miRNAs as transactions and target genes as their items. Support and confidence values are used to prune association rules on miRNA-target genes data to construct support based GCNs (sGCNs) along with support and confidence based GCNs (scGCNs). We use overlap analysis and the topological features for the performance analysis of GCNs. We also inf...
Source: Cellular and Molecular Biology - May 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Molecular analysis of endometrial inflammation in preterm birth.
Authors: Cecati M, Sartini D, Campagna R, Biagini A, Ciavattini A, Emanuelli M, Giannubilo SR Abstract Spontaneous preterm birth (sPTB) represents the 35%-45% of all preterm birth (PTB) cases and its etiology is unknown. We investigated if the expression level of endometrial cytokines and angiogenetic factors is related to the onset of sPTB.Endometrial tissues from non-pregnant women who experienced sPTB and from non-pregnant women who did not experience sPTB were collected and examined for their expression profile. With this aim, the PCR Array analysis was performed and data were confirmed by Real-Time PCR. Differ...
Source: Cellular and Molecular Biology - May 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Association of leukemia inhibitory factor gene polymorphism and in vitro fertilization outcome in a population in northern Iran.
In conclusion, the results of this study indicate that SNP 3951C/T of LIF may not be associated with IVF-ET outcome in this population. Although more studies should be considered with larger number of patients and control subjects to confirm our results. PMID: 28466814 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - May 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The study of CD14+HLA-DR-/low myeloid-drived suppressor cell (MDSC) in peripheral blood of peripheral T-cell lymphoma patients and its biological function.
Authors: Du J, Sun X, Song Y Abstract Peripheral T-cell lymphoma is the generic term of a group of heterogeneous disease in non-Hodgkin's lymphoma. To investigate the quantity of CD14+HLA-DR-/low MDSC and T cell subsets in peripheral blood of peripheral T cell lymphoma (PTCL) patients, and explore the biological functions of CD14+HLA-DR-/low MDSC in peripheral T-cell lymphoma. Flow cytometry was used to determine CD14+HLA-DR-/low MDSC and T cell subsets in peripheral blood of 33 peripheral T-cell lymphoma patients and 23 healthy cases; CD3 +T cells, CD14+HLA-DR-/low MDSC cells and HLA-DR+ cells were selected with m...
Source: Cellular and Molecular Biology - May 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Antioxidant effect of curcumin against exposure to malathion in Cyprinus carpio.
In conclusion, this study demonstrated that malathion caused oxidative stress and negative alterations on the antioxidant enzyme activities of the fish. However, this toxic effect was neutralised by the administration of curcumin. PMID: 28466816 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - May 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Dynamic observation of IL-33 and its receptors in HIV patients who received HAART.
Authors: He B, Zheng L, Zhou H, He Y, Chen Z, Xiao S, Wang H, Ling Y, Zheng Y Abstract The purpose of this study was to observe the changes in serum IL-33 and its soluble receptors, serum sST2 level and ST2L expression in PBMCs of HIV-infected patients receiving highly-active antiretroviral therapy (HAART) for 12 monthes. Fifty-five chronic HIV-1-infected adults were recruited for this study before initiation of HAART. 30 age and gender matched healthy adults were recruited as control. Blood was obtained from each patient at baseline (0 month) and 12 months after initiation of highly active antiretroviral therapy(H...
Source: Cellular and Molecular Biology - May 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Evaluation of relationship between aerobic fitness level and range of isocapnic buffering periods during incremental exercise test.
Authors: Algul S, Ozcelik O, Yilmaz B Abstract The purpose of this study is to examine the relationship between the amount of O2 uptake (VO2) in the range of isocapnic buffering (ICB) periods and aerobic fitness levels of subjects with different exercise tolerance levels. A total of 50 young male subjects (20.8±0.4 years) performed an incremental exercise test using a cycle ergometer to determinetheir anaerobic threshold (AT), respiratory compensation point (RCP) and maximal exercise capacity (Wmax). The ICB period is defined as the region between AT and RCP. Pulmonary gas exchange parameters were measured b...
Source: Cellular and Molecular Biology - May 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Familial Mediterranean Fever: Observations from a pilot gene expression microarray analysis study.
Authors: Mahfouz R, Kreidieh K, Khalek RA, Yazbek S Abstract Familial Mediterranean Fever (FMF) is an autosomal recessive autoinflammatory disease affecting people of Mediterranean ancestry. The disease is caused by mutations in the MEFV gene located on chromosome 16p13.3. The aim of this pilot study was to assess global gene expression and identify genes and pathways involved in FMF that could be downstream to MEFV mutations or could be novel involved. EDTA blood samples were collected from 14 patients showing FMF-like symptoms and age-matched to 7 controls showing healthy conditions. Microarray was used to assess...
Source: Cellular and Molecular Biology - May 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Association between BANK1 polymorphisms and susceptibility to autoimmune diseases: A meta-analysis.
This study aimed to explore whether BANK1 polymorphisms are associated with susceptibility to autoimmune diseases. We conducted a meta-analysis on the associations between the BANK1 rs10516487, rs3733197, and rs17266594 polymorphisms and autoimmune diseases. Twenty-two articles with a total of 22,684 patients and 36,437 controls were included in the meta-analysis. Meta-analysis revealed a significant association between autoimmune diseases and the BANK1 rs10516487 T allele (OR = 1.161, 95% CI = 1.092-1.275, p = 1.9 × 10-6, heterogeneity p
Source: Cellular and Molecular Biology - May 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Long non-codingRNA (lncRNA) TUG1 and the prognosis of cancer: a meta-analysis.
In conclusion, this meta-analysis study indicated that TUG1 might associate with the OS of osteosarcoma and digestive system's tumors. PMID: 28466821 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - May 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The effects of CDKN2A rs3731249, rs11515, and rs3088440 polymorphisms on cancer risk.
Authors: Dong Y, Wang X, Yang YW, Liu YJ Abstract Many studies have been carried out to examine whether there is an association between CDKN2A polymorphisms and cancer risk, but the results from these studies were controversial. In the present study, we performed a meta-analysis to clarify whether there is an association of CDKN2A polymorphisms and cancer risk. Published reports were searched in PubMed and Google Scholar. ORs with 95% CIs were calculated in the dominant models. Twenty six case-control studies that met the inclusion criteria were included in the final meta-analysis. Overall, we found that rs3731249,...
Source: Cellular and Molecular Biology - May 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The effects of melatonin administration on KCNQ and KCNH2 gene expressions and QTc interval in pinealectomised rats.
Authors: Ovali MA, Uzun M Abstract Melatonin is a hormone secreted from the pineal gland and has different cardiovascular effects. KCNQ genes expressed in aorta related with vascu- lar tone and KCNH2 gene characterised in left ventricle associated with QT duration. The aim of this study was to investigate the effects of melatonin on the regulation of the blood pressure and the relationships between the expressions of aorta KCNQ1-5, left ventricle KCNH2 genes and the QTc interval. For that purpose, 42 male adult Sprague-Dawley rats were devided into six groups; SHAM, SHAM+L-NAME, PLT, PLT+L-NAME, PLT+MEL and PLT+L-N...
Source: Cellular and Molecular Biology - May 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The effects of Nobiletin, Hesperetin, and Letrozole in a combination on the activity and expression of aromatase in breast cancer cells.
In this study, aromatase enzyme activity based on the conversion of androgen substrate testosterone to 17β-Estradiol was determined. Estradiol concentrations were measured using an electrochemiluminescence immunoassay. CYP19 gene expression was determined by quantitative real-time PCR. Our findings demonstrated that none of combinations including LET+NOB, LET+HES, LET+NOB+HES, and NOB+HES had no significant effects on aromatase activity and expression. The present study showed for the first time that the combination of HES, NOB, and LET had no effects on activity and expression of aromatase in MCF-7 breast cancer cell...
Source: Cellular and Molecular Biology - April 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The link between intergenic distances and controls exerted on the transcriptional regulation; an inferential approach.
Authors: Ahmadi M, Salehi B Abstract The position of genes along the genome is an important evolutionary factor for organizing gene regulation. Hence, transcriptional regulatory network have been studied much more extensively from gene distributions rather than other viewpoints. The systematics of intergenic distances, therefore, should be taken into account as an important source of information on the controls exerted on gene expression by various biological mechanisms. Here we study a collection of features including, intergenic and interoperonic distances, distances between isolated genes, distances between regu...
Source: Cellular and Molecular Biology - April 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Methyl thiophanate-induced toxicity in liver and kidney of adult rats: a biochemical, molecular and histopathological approach.
Authors: Feki A, Ben Saad H, Jaballi I, Magne C, Boudawara O, Zeghal KM, Hakim A, Ben Ali Y, Ben Amara I Abstract The aim of this study was to elucidate the redox effects of Thiophanate methyl (MT) in the rat liver and kidney. Our results showed, after 3 days of MT injection (700 mg/kg), an increase in malondialdehyde (MDA), hydrogen peroxide and advanced oxidation protein products levels. Glutathione peroxidase and superoxide dismutase activities were also remarkably increased in the liver but decrease in the kidney. Glutathione and vitamin C values were significantly reduced. The changes in biochemical parameters...
Source: Cellular and Molecular Biology - April 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The G-protein alpha-subunit gene CGA1 is involved in regulation of resistance to heat and osmotic stress in Chlamydomonas reinhardtii.
Authors: Lee CS, Ahn W, Choi YE Abstract In eukaryotic cells, many important functions of specific G-proteins have been identified, but microalgal G-proteins are poorly studied. In this work, we characterized a gene (CGA1) encoding the G-protein α-subunit in Chlamydomonas reinhardtii. Independent knockdown mutants of CGA1 were generated via RNA interference (RNAi). CGA1 expression levels were consistently and significantly reduced in both independent CGA1 mutant cell lines (cga1). Both cga1 mutants had a higher survival rate at 35°C in comparison with the wild type. This stronger resistance of the cga1 mu...
Source: Cellular and Molecular Biology - April 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

miRNA and mRNA expression profiling in rat brain following alcohol dependence and withdrawal.
In conclusion, consistent and integrated variations in miRNA expression and in their focus mRNAs in rat brain were noted after alcohol exposure and withdrawal. Besides, understanding the molecular mechanisms of alcohol abuse will no doubt guide to development of significant cure methods for addiction. We are of the opinion that our findings may shed light on classification of novel biomarkers. PMID: 28364783 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - April 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

TTY2 genes deletions as genetic risk factor of male infertility.
This study was performed on 45 infertile males with idiopathic azoospermia without any AZF micro deletions (group A), 33 infertile males with azoospermia which do not screened for AZF micro deletions (group B) and 65 fertile males (group C), from October 2013 to April 2015 in west of Iran. Polymerase chain reaction (PCR) method was used for detection of TTY2L12A and TTY2L2A gene deletions in studied groups. No deletions were detected in normal fertile males of group C. 1 out of 45 azoospermic males of group A (2.22%) and 3 out of 33 azoospermic males of group B (9.09%) had TTY2L2A deletion (p= 0.409 and p= 0.036 respective...
Source: Cellular and Molecular Biology - April 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Stable silencing of IGF1R using Lentiviral-mediated shRNA in HEK293T cells.
Authors: Nasehi L, Ghahremani MH, Yavari K, Hadjati J, Fallah A, Abdolhossein Zadeh B, Saltanatpour Z Abstract Insulin-like growth factors are among the peptide mitogens that regulate cell proliferation and differentiation as well as mediator of antiapoptotic signals. The imbalance between the expression and activities of these molecules may lead to malignancy in cells. Evidences have suggested the insulin-like growth factor 1 receptor (IGF-1R) signaling pathway as a therapeutic target in the management and treatment of cancer. In this present study, we have generated silencing stable clones of HEK cells using six ...
Source: Cellular and Molecular Biology - April 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Dandelion-enriched diet of mothers alleviates lead-induced damages in liver of newborn rats.
This study evaluated the hepatic toxic effects of prenatal exposure to lead in rats and the possible protective effect of dandelion (Taraxacum officinale) added to the diet. Female rats were given a normal diet (control) or a diet enriched with dandelion (treated). In addition, lead acetate was administered to half of the rats through drinking water from the 5th day of gestation until the 14th day postpartum. Lead toxicity was evaluated in their offspring by measuring body and liver weights, plasma biochemical parameters, liver damage, as well as protein content and activities of antioxidant enzymes in the liver tissues. L...
Source: Cellular and Molecular Biology - April 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

TGF ß3 / SfaN1 gene variant and the risk factor of nonsyndromic cleft palate only among Indonesian patients.
In conclusion, TGFβ3 gene / SfaN1 polymorphism can be considered as the risk factor associated with NS CPO in Indonesian patients. PMID: 28364787 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - April 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The clinical research of Thinprep Cytology Test (TCT) combined with HPV-DNA detection in screening cervical cancer.
Authors: Liu Y, Zhang L, Zhao G, Che L, Zhang H, Fang J Abstract Our objective is to explore the clinical value of thinprep cytologic test (TCT) combined with HPV-DNA detection in screening cervical cancer. 420 cervical cancer patients admitted in our hospital between April, 2011-April, 2014 were selected. All patients received TCT and HPV-DNA detection, and cervical tissue biopsy was used to confirm the diagnosis. TCT screening results showed that there were 175 patients were >ASCUS and the positive rate was 41.7%, histopathological screening showed that there were 199 patients were ≥cervical intraepithe...
Source: Cellular and Molecular Biology - April 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research