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Investigation of some DNA repair genes association in non small cell lung cancer.
Authors: Coskunpinar E, Yildiz P, Aynaci E, Turna A, Musteri Oltulu Y, Hekimoglu E, Isbir T, Yaylim I Abstract Ribonucleoside-diphosphate reductase subunit M2, also known as ribonucleotide reductase small subunit, is an enzyme that in humans is encoded by the RRM2 gene and also Ribonucleoside-diphosphate reductase large subunit is an enzyme that in humans is encoded by the RRM1 gene. RRM1 is a gene important in determining tumor phenotype, but also induced the expression of PTEN tumor suppressor gene, cell migration, invasion and metastasis formation, and play a preventive role. ERCC2 DNA repair mechanism is associ...
Source: Cellular and Molecular Biology - January 13, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Assessment of wild mint from Tunceli as source of bioactive compounds, and its antioxidant Activity.
Authors: Turkoglu S Abstract The types of wild mint (Mentha spicata L.) were sampled from different geographical regions in Tunceli (Turkey) in order to find out their vitamin, mineral, phenolic contents and their antioxidant properties. The total phenol varied from 77.7±0.242 to 52.34±0.351 mg of GAEs/g of dry mint. The highest radical effect of scavenging was observed in Mazgirt parting of the ways 7.5 km with 6.17±0.245 mg/mL. The highest reducing power and metal chelating were observed in the mint from Cicekli parting of the ways 6.5 km Demirkapı. Among the various macronutrients which wer...
Source: Cellular and Molecular Biology - January 13, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Association study of hsa-mir-603 rs11014002 polymorphism and risk of breast cancer in a sample of Iranian population.
In conclusion, the findings indicated that Mir603 rs11014002 T allele might contribute to decrease the risk of BC in a sample of Iranian population. Further studies with larger sample sizes and different ethnicities are warranted to confirm our findings. PMID: 26718432 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - January 13, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Conformational stability, spectroscopic and computational studies, hikes' occupied molecular orbital, lowest unoccupied molecular orbital, natural bond orbital analysis and thermodynamic parameters of anticancer drug on nanotube-A review.
Authors: Ghasemi AS, Mashhadban F, Hoseini-Alfatemi SM, Sharifi-Rad J Abstract Today the use of nanotubes (CNTs) is widely spread a versatile vector for drug delivery that can officiate as a platform for transporting a variety of bioactive molecules, such as drugs. In the present study, the interaction between the nanotube and anticancer drugs is investigated. Density functional theory (DFT) calculations were using the Gauss view and the complexes were optimized by B3LYP method using B3LYP/6-31G (d, p) and B3LYP/6-311++G (d, p) basis set in the gas phase and water solution at 298.15K. The calculated hikes' occupied...
Source: Cellular and Molecular Biology - January 13, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Increased pregnancy rate using standardized coculture on autologous endometrial cells and single blastocyst transfer : a multicentre randomized controlled trial.
We present the results of the intermediate analysis that resulted in the study ending considering the observed difference. Three hundred thirty nine patients were randomized: 170 in the AECC group and 169 in the control group. The clinical PR per transfer was 53.4% with AECC and 37.3% in the control group (p=0.025). The quality of embryos was improved with AECC. These results suggest that implementation of the AECC technique to a large number of In-Vitro Fertilization (IVF) centres could lead to a substantial improvement in the proportion of successful assisted reproduction. The study was supported by the Laboratoires Gen&...
Source: Cellular and Molecular Biology - January 13, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Determination of effective miRNAs in wound healing in an experimental Rat Model.
Authors: Coskunpinar E, Arkan H, Dedeoglu BG, Aksoz I, Polat E, Araz T, Aydos A, Oztemur Y, Akbas F, Onaran I Abstract The larvae of Lucilia sericata have been used for centuries as medicinal maggots in the healing of wounds. The present study aimed to screen potential microRNAs related to ES-induced wound healing in rat skin wounds and to investigate the potential mechanisms contributing to accelerated wound healing. Healthy, male, 12 weeks old Wistar albino rats weighing 250-300 g were supplied by the Animal Experimental Center. All animal studies were performed in accordance with the NIH Guide for the Care and U...
Source: Cellular and Molecular Biology - January 13, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Association between functional CD24 polymorphisms and susceptibility to autoimmune diseases: A meta-analysis.
This study aimed to explore whether the functional CD24 A57V and TG/del polymorphisms are associated with susceptibility to autoimmune diseases. A meta-analysis was conducted on the associations between the CD24 A57V and TG/del polymorphisms and autoimmune diseases using (1) allele contrast, and (2) the recessive, (3) dominant, and (4) co-dominant models. Twenty-six comparative studies with 7,507 patients and 8,803 controls were included in the meta-analysis. The meta-analysis revealed a significant association between autoimmune disease and the CD24 Val allele (OR = 1.285, 95% CI = 1.177-1.403, p = 1.0 × 10-9). Meta...
Source: Cellular and Molecular Biology - January 13, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Associations between interleukin-1 and IL-1 receptor antagonist polymorphisms and susceptibility to rheumatoid arthritis: A meta-analysis.
This study determined whether interleukin-1 (IL-1) polymorphisms are associated with susceptibility to rheumatoid arthritis (RA). A meta-analysis was conducted on the associations between the IL-1A, IL-1B, and IL-1 receptor antagonist (IL-1RN) polymorphisms and RA. A total of 16 studies involving 4,339 RA cases and 3,885 controls were included in the meta-analysis. Meta-analysis of the IL-1B -511 C/T polymorphism revealed an association between the IL-1B -511 T allele and RA in Caucasians (OR = 0.913, 95% CI = 0.840-0.992, p = 0.031), but not in Asians. Ethnicity-specific meta-analysis indicated an association between the ...
Source: Cellular and Molecular Biology - January 13, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Identification and characterization of chalcone synthase cDNAs (NnCHS) from Nelumbo nucifera.
In this study, six full-length cDNAs (NnCHS) encoding CHS from Nelumbo nucifera were successfully isolated, using rapid amplification cDNA end (RACE) assay. The obtained cDNAs were 1426 bp in size, containing a 1167 bp open reading frame coding 389 amino acids. Exons-intron architecture of NnCHS gene was illustrated, consisting two exons inserted by a 426 bp intron. The putative NnCHS possessed all the conserved active sites for CHS function as well as the family signature. Phylogenetic analysis revealed that NnCHS shared high homology with CHS from high plants, and the homology-based structural modeling showed that NnCHS ...
Source: Cellular and Molecular Biology - January 13, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Differential effects of peroxisome proliferator-activated receptor agonists on doxorubicin-resistant human myelogenous leukemia (K562/DOX) cells.
Authors: Yousefi B, Samadi N, Baradaran B, Rameshknia V, Shafiei-Irannejad V, Majidinia M, Targhaze N, Zarghami N Abstract P-glycoprotein (P-gp)-mediated multidrug resistance (MDR) in tumor cells is still a main obstacle for the chemotherapeutic treatment of cancers. Therefore, identification of safe and effective MDR reversing compounds with minimal adverse side effects is an important approach in the cancer treatment. Studies show that peroxisome proliferator-activated receptor (PPARs) ligands can inhibit cell growth in many cancers. Here, we investigated the effect of different PPAR agonists include fenofibrate,...
Source: Cellular and Molecular Biology - January 13, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Cloning, Prokaryotic Expression and Purification of CpfS1 Gene from Arabidopsis Thaliana.
Authors: He Q, Fu AY, Zhang GC, Li TJ, Zhang JH Abstract CpfS1 Gene cloned from arabidopsis thaliana was expressed in Escherichia coli DH5α. A cDNA fragment about 320 bp was amplified from the total RNA of arabidopsis thaliana seeds by reverse transcription PCR (RT-PCR) with a pair of specific primers based on the sequences of the AtCpfS1 gene. The recombinant prokaryotic expression vector pET30a-AtCpfS1 was constructed by inserting the cDNA fragment encoding the mature peptide into the prokaryotic expression vector pET30a, and then transformed into E. coli DH5α. Sequence analysis showed that the fragme...
Source: Cellular and Molecular Biology - January 13, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The cytotoxic activity of Ziziphus Jujube on cervical cancer cells: In Vitro study.
Authors: Hoshyar R, Jamali S, Fereidouni M, Abedini MR Abstract Recently, there are tendency to use natural products such as Ziziphus Jujube (Jujube) as therapeutic agents for cancer. Understanding the molecular mechanisms of anti-cancer effects of Jujube may improve the current therapeutic strategies against cervical cancer. Our MTT data showed a significant dose- and time-dependent inhibition of OV-2008 cell proliferation following Jujube administration. Moreover, qRT-PCR analyses significantly revealed the suppression of cyclin D1 and the enhancement of P53, P21 and P27 expression in treated cells. These results...
Source: Cellular and Molecular Biology - January 13, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Retraction: Arabidopsis thaliana SEPALLATA3 protein prokaryotic expression and purification.
Authors: Cellular and Molecular Biology Staff Abstract [This delete the article DOI: 10.14715/cmb/2015.61.2.11.]. PMID: 26718597 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - January 13, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Retraction: Effect of Tongxinluo on nerve regeneration in mice with diabetic peripheral neuropathy.
Authors: Cellular and Molecular Biology Staff Abstract [This delete the article DOI: 10.14715/cmb/2015.61.5.17.]. PMID: 26690972 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - December 24, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Endonuclease G depletion may improve efficiency of first generation adenovirus vector DNA replication in HeLa cells.
In this study, we tried to increase the efficiency of Ad5 ΔE1,E3 DNA replication in the cervical carcinoma HeLa cells by using RNA interference (RNAi) to target endonuclease G (EndoG) whose depletion leads to an accumulation of cells in the G2/M transition. Targeting of EndoG by an shRNA encoded on an Ad5 ΔE1,E3 vector resulted in an early proliferation defect of cervical carcinoma HeLa cells. This effect coincided with enhanced DNA replication and encoded transgene expression of an Ad5 ΔE1,E3 vector. Applied in high concentrations, the EndoG-targeting Ad5 ΔE1,E3 vector showed enhanced HeLa cell kil...
Source: Cellular and Molecular Biology - December 18, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Trans-resveratrol induces a potential anti-lipogenic effect in lipopolysaccharide-stimulated enterocytes.
Authors: Etxeberria U, Castilla-Madrigal R, Lostao MP, Martínez JA, Milagro FI Abstract A DNA microarray analysis was conducted in Caco-2 cells to analyse the protective effects of trans-resveratrol on enterocyte physiology and metabolism in pro-inflammatory conditions. Cells were pre-treated with 50 μΜ of trans-resveratrol and, subsequently, lipopolysaccharide (LPS) was added for 48 h. The microarray analysis revealed 121 genes differentially expressed between resveratrol-treated and non-treated cells (B> 0, is the odd thatthe gene is differentially expressed). Inhibitor of DNA binding 1 (ID1), his...
Source: Cellular and Molecular Biology - December 18, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

CD45RO+ memory T-cells produce IL-17 in patients with atherosclerosis.
Authors: Behnamfar N, Zibaeenezhad MJ, Golmoghaddam H, Doroudchi M Abstract Several CD4+ T helper (Th) cell subsets are shown to play a role in atherosclerotic lesion formation and progression. We investigated the frequencies of IL-17 and IFN-γ producing CD4+ T-cell subsets in the peripheral blood mononuclear cells (PBMCs) of 10 patients with atherosclerosis and 6 individuals with normal/insignificant coronary artery disease. Th1 and Th17 memory and effector T-cells were enumerated by flowcytometry and correlated with the clinical data and lipid profiles of the subjects. We found the ex-vivo (P=0.0001) and in...
Source: Cellular and Molecular Biology - December 18, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Association between a Functional HLA-G 14-bp Insertion/deletion Polymorphism and Susceptibility to Autoimmune Diseases: A Meta-analysis.
Authors: Lee YH, Bae SC Abstract The aim of this study was to determine whether a functional human leukocyte antigen-G (HLA-G) 14-bp insertion (I)/deletion (D) polymorphism is associated with susceptibility to autoimmune diseases. A meta-analysis was conducted to assess the association between an HLA-G 14-bp I/D polymorphism and autoimmune diseases using 1) allele contrast, as well as 2) recessive, 3) dominant, and 4) codominant models. Sixteen articles that included 20 comparative studies with 3,555 patients and 5,225 controls were included in the meta-analysis. These studies were performed on nine Caucasian, six ...
Source: Cellular and Molecular Biology - December 18, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Regulation of ATRIP protein abundance by RAD9 in the DNA damage repair pathway.
Authors: Peng XJ, Liu SJ, Bao CM, Liu YZ, Xie HW, Cai YH, Li BM, Hang HY, Ding X Abstract Genotoxic stress activates checkpoint signaling pathways that activate the checkpoint kinases ATM and ATR, halt cell cycle progression, and promote DNA repair. A number of proteins act in concert with ATR to phosphorylate Chk1, including RAD17, the RAD9-RAD1-HUS1 complex, ATR/ATRIP and TopBp1. However, how these proteins involved act in concert with one another to propagate and maintain the checkpoint response is not well understood. Here, we reported that upregulation of RAD9 protein increased the quantity of ATRIP, suggestin...
Source: Cellular and Molecular Biology - December 18, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Role of Berberine on molecular markers involved in migration of esophageal cancer cells.
In this study, we investigated the effect of berberine on an esophageal cancer cell line, KYSE-30 with emphasis on its effects on the expression of certain chemokine receptors. The cytotoxic effect of berberine on KYSE-30 cells was analyzed by MTT assay. In vitro cell migration assay was also applied to the treated cells and the expression levels of the selected chemokine receptors (CXCR4 and CCR7) was measured at mRNA level. A retarded growth, associated with increasing concentrations of berberine, was obvious. On the other hand, the migration rate of the cells was decreased when they were treated with different concentra...
Source: Cellular and Molecular Biology - December 18, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

MicroRNA-424 suppresses estradiol-induced cell proliferation via targeting GPER in endometrial cancer cells.
In this study, we confirmed that cell proliferation is increased significantly in E2-treated endometrial cancer cell lines. Moreover, miR-424 overexpression dramatically decreased E2-induced cell proliferation, indicating a pivotal role in endometrial cancer cell growth. In addition, the results suggest that miR-424 up-regulation inactivated the PI3K/AKT signaling, which was mediated by G-protein-coupled estrogen receptor-1 (GPER) in endometrial cancer. Furthermore, the luciferase report confirmed the targeting reaction between miR-424 and GPER. After transfection with the GPER overexpression vector into E2-induced endomet...
Source: Cellular and Molecular Biology - December 9, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effects of shRNA-mediated knockdown of SPOCK1 on ovarian cancer growth and metastasis.
In this study, we assessed the potential of SPOCK1 as a significant biomarker for ovarian cancer development. We determined that the expression of SPOCK1 was evidently high in ovarian cancer tissues and the cell lines OVCAR3 and SKOV3. The knockdown of SPOCK1 by specific shRNA significantly inhibited cell proliferation and colony formation in both OVCAR3 and SKOC3 cells. In a xenograft model of ovarian cancer, the mice implanted with SPOCK1 knockdown SKOV3 cells exhibited a slower tumor growth rate. The dissected tumors also weighed less in the SPOCK1-depleted mice group. Furthermore, the knockdown of SPOCK1 evidently inhi...
Source: Cellular and Molecular Biology - December 9, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Molecular structure, spectroscopic assignments and other quantum chemical calculations of anticancer drugs - A review.
Authors: Ghasemi AS, Deilam M, Sharifi-Rad J, Ashrafi F, Hoseini-Alfatemi SM Abstract In many texts, both theoretical and experimental studies on molecular structure and spectroscopic assignments of anticancer medicines have been reported. Molecular geometry parameters have been experimentally obtained by x-ray structure determination method and optimized using computational chemistry method like density functional theory. In this review, we consider calculations based on density function theory at B3LYP/6-31G (d,p) and B3LYP/6-311++G (d,p) levels of theory. Based on optimized geometric parameters of the molecules,...
Source: Cellular and Molecular Biology - December 9, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Molecular Characterization and TRAP Analysis of Gene in Dendranthema morifolium.
Authors: Luo Y, Li Q, Wang X, Yang F, Nong S, Li Q, Zhu D Abstract The molecular maker system - TRAP was applied to develop a novel and more accurate method to identify the variety and establish the evolutionary relationship of different categories of Dendranthema morifolium. A software - GENESIS 2.4 was used to conduct the cluster analysis and genetic dendrogram establishment. The results showed that 202 different fragments were amplified with 6 pair primers using the TRAP marker system. The polymorphic fragments number is 45, which takes up to 22.3%. The cluster analysis showed that 4 materials used in this study...
Source: Cellular and Molecular Biology - December 9, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Wogonin increases doxorubicin sensitivity by down-regulation of IGF-1R/AKT signaling pathway in human breast cancer.
Authors: Fu P, Du F, Liu Y, Hong Y, Yao M, Zheng S Abstract Currently drug resistance has remained a major challenge in successful breast cancer therapy. Wogonin, one of the active components of scutellaria baicalensis, has shown anticarcinogenic, chemopreventive, and immunoregulatory functions. The present study aimed to explore whether wogonin exerted synergistic cytotoxicity with doxorubicin in breast cancer. Our data indicated that wogonin inhibited the proliferation of breast cancer cells in a dose- and time-dependent manner. Combined treatment with wogonin increased the doxorubicin sensitivity in breast cance...
Source: Cellular and Molecular Biology - December 9, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Induction of apoptosis by pistachio (Pistacia vera L.) hull extract and its molecular mechanisms of action in human hepatoma cell line HepG2.
Authors: Fathalizadeh J, Bagheri V, Khorramdelazad H, Kazemi Arababadi M, Jafarzadeh A, Mirzaei MR, Shamsizadeh A, Hajizadeh MR Abstract Several important Pistacia species such as P. vera have been traditionally used for treating a wide range of diseases (for instance, liver-related disorders). There is a relative lack of research into pharmacological aspects of pistachio hull. Hence, this study was aimed at investigating whether pistachio rosy hull (PRH) extract exerts apoptotic impacts on HepG2 liver cancer cell line. In order to evaluate cell viability and apoptosis in response to treatment with the extract, MTT...
Source: Cellular and Molecular Biology - December 9, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Fibronectin chorused cohesion between endothelial progenitor cells and mesenchymal stem cells of mouse bone marrow.
In this study, we investigated the mechanism of cohesion between MSCs and EPCs. And demonstrated that fibronectin (FN) in EPCs activated the integrin α5β1 of MSCs and further mediated cell-cell cohesion. Integrin α5β1 and its FN ligand played critical roles not only in single—cell line adhesion, but also in adhesion between stem and niche cells. This novel finding is important to understand the cross—talk between MSCs and their niche cells. PMID: 26025398 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Molecular events are associated with resistance to vinblastine in bladder cancer.
Authors: Chen Q, Chong T, Yin J, Luo P, Deng A Abstract Bladder cancer occurs in the majority of cases in males, which represents the fourth highest incident cancer in men and tenth in women. It is associated with a high rate of recurrence, and prognosis is poor once the cancer metastasizes to distant sites. Transitional cell cancer (TCC) is the most predominant histological type. Bladder cancer is highly chemosensitive. However, the presence of acquired drug resistance is one of the primary impediments to the success of chemotherapy. To differentiate and delineate the molecular events, we developed drug resistant ...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Vitis labrusca leaf extract prevents pentylenetetrazol-induced oxidative damage but not seizures in rats.
Authors: Coitinho AS, da Costa BM, Dos Santos MT, Fank B, Hackmann CL, Salvador M, Dani C Abstract Epilepsy is a disorder of the central nervous system characterized by recurrent seizures. It is a very common disease in which approximately 30% of patients do not respond favorably to treatment with anticonvulsants. Oxidative stress is associated with neuronal damage arising from epileptic seizures. The present study investigated the possible anticonvulsant and antioxidant effects of a leaf extract of Vitis labrusca in an animal model of seizures induced by pentylenetetrazole (PTZ). The animals received injections of...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Comparison of two codon optimization strategies enhancing recombinant Sus scrofa lysozyme production in Pichia pastoris.
Authors: Zhu D, Cai G, Wu D, Lu J Abstract Lysozyme has played an important role in animal feed additive industry, food additive industry and biological engineering. For improving expression efficiency of recombinant lysozyme from Sus scrofa, two genes respectively designed by the most used codon optimization strategies, "one amino acid one codon" and "codon randomization", were synthesized and expressed in Pichia pastoris X—33. At shaking flask level, Sus scrofa lysozyme (SSL) under two conditions had a highest activity of 153.33±10.41 and 538.33±15.18 U/mL after a 5 day...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Differentiation of transforming growth factor β1-induced mesenchymal stem cells into nucleus pulposus-like cells under simulated microgravity conditions.
This study aims to observe the differentiation effects of transforming growth factor β1 (TGF-β1)-transfected bone marrow mesenchymal stem cells (BMMSCs) into the nucleus pulposus-like cells under simulated microgravity conditions. BMMSCs were isolated from the adult rabbits, then cultured and proliferated to the third generation. After transfected by TGF-β1, these MMSCs were incubated in the calcium alginate gel microspheres and rotary cell culture system. The content changes of TGF-β1 inside BMMSCs, as well as the proliferation abilities of BMMSCs, were detected. The immunohistochemistry and toluidine ...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Oligodendrocyte progenitor cells differentiation of nuclear transferred mouse embryonic stem cells.
Authors: Salehi M, Ardeshirylajimi A, Mossahebi-Mohammadi M, Kondori Z, Jorjani M Abstract Central nervous system (CNS) injuries cause to variable disorders in people around the world without any decisive treatment. Use of embryonic stem cells (ESCs) would be helpful in repairing of neural system damages. Somatic cell nuclear transfer (SCNT) is a way for implanting ESCs with lowest possible rejection. In the present study, mouse nuclear transfers ESCs (ntESCs) ability in differentiation to oligodendrocyte progenitor cells (OPCs) was investigated by morphological study, RT—PCR and flow cytometry analysis. ...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Arabidopsis thaliana SEPALLATA3 protein prokaryotic expression and purification.
Authors: He Q, Fu AY, Zhang GC, Li TJ, Zhang JH Abstract SEPALLATA3 (SEP3) can be attributed to E class gene of the ABCE model of floral organ development. In order to reveal how SEP3 proteins form polymers, and the relationship between the polymers and their biological functions, the experiments of Arabidopsis thaliana AtSEP3 protein soluble expression in vitro were performed to construct a vector of prokaryotic expression, and investigate induced expression of recombinant proteins in Escherichia coli cells. The protein soluble expression was analyzed through the aspects of different protein domains, induction tim...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Estrogen deficiency inducing shifted cytokines profile in bone marrow stromal cells inhibits Treg cells function in OVX mice.
Authors: Zeng QM, Liu DC, Zhang XC, Wu JP, Ding L, Meng DH, Zhang J Abstract The study aims to reveal the effect of estrogen deficiency on Treg cells population in bone marrow in the development of osteoclastogenis with comparing the differences about Treg cells phenotypes and cytokines related with the homeostasis and functions maintenance of Treg cells in bone marrow in OVX mice and health control group. Wide—type C57BL/6 mice were operated OVX to mimic estrogen deficiency in PMO women. Treg cells population and their surface markers expressions were detected by flow cytometry. Cytokines profiles in bon...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Regulation of the Heme Oxygenase-1/carbon monoxide system by hydrogen sulfide in murine coxsackievirus B3-induced myocarditis.
Authors: Zhang S, Wu T, Xia T, Rong X, Wu T, Chu M, Wu R Abstract To explore the impact of hydrogen sulfide (H2S) on the heme oxygenase—1 (HO—1)/carbon monoxide (CO) system in coxsackie virus B3 (CVB3)—induced myocarditis. A total of 80 Balb/c mice were divided randomly into four groups designated N, C, P and S. Group N served as the negative control while groups C, P, and S were infected with CVB3 to induce myocarditis. Group P was additionally treated with DL—propargylglycine (PAG) to inhibit the generation of H2S while Group S was treated with NaHS, an H2S donor. Ten days ...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The association of PON1 192 Q/R polymorphism and the risk of female infertility.
Authors: Mashayekhi F, Behrouzi S, Yousefi M, Salehi Z Abstract Infertility is a disease of the reproductive system defined by the failure to achieve a clinical pregnancy after 12 months of regular unprotected sexual intercourse. Both environmental and genetic factors are involved in female infertility. Paraoxonase (PON) is an oxidant enzyme which plays an important role in various diseases and is associated with inflammation, oxidative stress and lipid metabolism. The present study was aimed to evaluate the PON1 192 Q/R gene polymorphism in female infertility. Samples were obtained from 150 patients diagnosed with...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

MiR-30a suppresses non-small cell lung cancer progression through AKT signaling pathway by targeting IGF1R.
This study investigated the expression and role of miR—30a in human NSCLC. The expression of miR—30a is significantly decreased in clinical NSCLC tissues and cell lines. Overexpression of miR—30a inhibited NSCLC cell proliferation, G1/S and S/G2 transition in vitro, whereas suppression of miR—30a facilitated NSCLC cell proliferation, G1/S and S/G2 transition. Using a luciferase reporter assay, insulin—like growth factor 1 receptor (IGF1R) was determined to be a direct target of miR—30a. Furthermore, silencing IGF1R resulted in the same biologic effects of miR&...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Investigation of cell aggregation on the substrate of a parallel-plate flow chamber.
Authors: Bai F, Dong C, Cao C, Sun R Abstract Adhesion of tumor cells (TCs) to polymorphonuclear neutrophils (PMNs) tethered on a monolayer of vascular endothelial cells (ECs) in shear flows is investigated theoretically. Both TC—PMN and PMN—EC aggregations are modeled using the population balance equations (PBEs). Parameters in the model obtained by curve fitting show that an increase of shear rate or viscosity will suppress the formation of aggregates and promote the breakage of them. Analysis on the collision frequency suggests that the averaged encounter duration is affected by viscosity. Ba...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Evaluation of FGFR2 gene polymorphism in women with breast cancer.
Authors: Salehi Z, Afzali S, Shabanipour S, Rahimi A Abstract Breast cancer (BC) is one of the most frequent cancers among women worldwide. It is a complex polygenic disorder that genetic factors play an important role in disease etiology. The highly significant association of the Fibroblast growth factor receptor 2 (FGFR2) locus with breast cancer risk has been replicated in multiple genome association study; however, it's mechanism of action remains unclear. The aim of this study was to investigate whether the single nucleotide polymorphism (SNP) C—906T within intron 2 of FGFR2 is responsible for susceptibi...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

siRNA-mediated silencing of MDR1 reverses the resistance to oxaliplatin in SW480/OxR colon cancer cells.
Authors: Montazami N, Kheir Andish M, Majidi J, Yousefi M, Yousefi B, Mohamadnejad L, Shanebandi D, Estiar MA, Khaze V, Mansoori B, Baghbani E, Baradaran B Abstract One of the most challenging aspects of colon cancer therapy is rapid acquisition of multidrug resistant phenotype. The multidrug resistance gene 1 (MDR1) product, p—glycoprotein (P—gp), pump out a variety of anticancer agents from the cell, giving rise to a general drug resistance against chemotherapeutic agents. The aim of this study was to investigate the effect of a specific MDR1 small interference RNA (siRNA) on sensitivity of ox...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Success of the PCR-based replication assay depends on the number of methylation sensitive restriction sites in the PCR amplifying region.
Authors: Metta MK, Tantravahi S, Kunaparaju R Abstract The PCR—based replication assay is one of the most simple, quick and economical methods for the analysis of episomal replication. However, in spite of its advantages the method has not been able to replace the southern—based replication assay, the latter of which is a tedious and time—consuming process. This is due to the generation of spurious amplification products in the PCR—based replication assay. The replication assay is based on the use of methylation—sensitive restriction endonucleases (eg. DpnI, MboI) t...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Letter to Editor: Cytochrome b gene expression in down syndrome subjects.
Authors: Salemi M, Barone C, Romano C, Grazia Salluzzo M, Galati Rando RG, Scavuzzo C, Salluzzo R, Russo R, Giambirtone M, Scillato F, Romano C PMID: 26068911 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Capacity of a newly isolated fungus Pleurotus eryngii from Tunceli, Ovacik for chemical oxygen demand reduction and biodecolorization of Azo-Dye Congo Red.
Authors: Yildirim N, Gonen U Abstract Biodecolorization of Congo red dye in both agar—plate and agitated liquid culture mediums by newly isolated white rot fungus Pleurotus eryngii has been studied. This fungus isolated from Tunceli—Ovacik province of Turkey. We have also examined the chemical oxygen demand reduction after decolorization under agitated liquid culture medium. For agar plate screening the decolorization capacity of P. eryngii, growth and decolorization halos were determined on saboroud dextrose agar (SDA) plates containing 0.05, 0.1, 0.5, 1 and 2 g/l of Congo red. P. eryngii showe...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

miR-145 regulates chemoresistance in hepatocellular carcinoma via epithelial mesenchymal transition.
Authors: Ju BL, Chen YB, Zhang WY, Yu CH, Zhu DQ, Jin J Abstract Resistance to chemotherapeutic drugs is a major obstacle in hepatocellular carcinoma (HCC) therapy. MicroRNA—145 (miR—145) has been shown to be down—regulated in several cancers and may be involved in the process of carcinogenesis. The present study aimed to evaluate the effects of miR—145 in adriamycin (ADM)—resistant human HCC cells. We found that miR—145 was significantly reduced in HepG2/ADM and HuH7/ADM cells compared with the chemosensitive parental cells. Up—regulation of miR...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

MRJF4, a novel histone deacetylase inhibitor, induces p21 mediated autophagy in PC3 prostate cancer cells.
Authors: Di Giacomo V, Di Valerio V, Rapino M, Bosco D, Cacciatore I, Ciulla M, Marrazzo A, Fiorito J, Di Stefano A, Cataldi A Abstract Autophagy is a cellular defense mechanism which occurs through degradation and recycling of cytoplasmic constituents and represents a caspase—independent alternative to cell death by apoptosis. It is generally accepted that the suppression of autophagy in many cancer cells is directly correlated to malignancy; hence, the control of autophagy genes could represent a target for cancer therapy. The inhibition of cell proliferation through autophagy activation could be an imp...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

microRNA-33A expression is reduced in cerebral cortex in a rat model of ischemic tolerance.
This study examined the microRNA—33a (miR—33a) expression levels in cerebral cortex in rat model of cerebral ischemic tolerance (IT), with 3—nitropropionic acid (3—NPA) preconditioning. Rat model of cerebral IT was established as follows: 14 male Sprague—Dawley rats were randomly divided into two groups, the 3—NPA treated group and the control group. Intraperitoneal injection of 3—NPA or normal saline was performed in 3—NPA treated group and control group, respectively. Middle cerebral artery occlusion (MCAO) was performed in all the rats 3 day...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The effect of moderately halophilic bacteria supernatant on proliferation and apoptosis of cancer cells and mesenchymal stem cells.
Authors: Sarvari S, Seyedjafari E, Amoozgar MA, Bakhshandeh B Abstract Many drug discoveries and developing of their applications has originated from microbial metabolites. The most efforts in development of new drugs are concerned with anti—cancer agents that cause better treatment results, less side effects, and more economical production. Several anti—tumor drugs have been recently extracted from natural microbial products. Among these various microbial diversity, Marin bacteria and Archaea have been considered as important and efficient organisms to serve as manufacturers of diverse bioactive compou...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Vitamin D Deficiency, Obesity and Diabetes.
Authors: Li YX, Zhou L Abstract Obesity and type 2 diabetes mellitus (T2DM) are main chronic diseases harming human health. Although the association between obesity and T2DM is well established, the molecular mechanism is still unclear. Accumulating evidence suggests vitamin D plays a role in the development of these diseases. Vitamin D is a necessary nutrient for humans. People usually do not pay attention to supplementing vitamin D, since vitamin D can be produced when their skin is exposed to the sunlight. Nevertheless, even in highly sunny regions, vitamin D deficiency exists, suggesting vitamin D deficiency is...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Regional and cellular distribution of monocarboxylate transporters 13 and 14 in the cattle gastrointestinal tract.
This study is the first to describe the precise cellular localization of the orphan monocarboxylate transporters (MCT13) and (MCT14) in the physiologically distinct regions of the gastrointestinal tract of mammals. The present study demonstrated conclusively the regional distribution and relative expression levels of MCT13 and MCT14 on both mRNA and protein levels in the cattle gastrointestinal tract. The mRNA expression levels of MCT13 and MCT14 in the rumen, abomasum, jejunum, cecum, and proximal colon of cattle were examined using quantitative real time—PCR analysis. The precise cellular location of MCT13 and ...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

TLR3 and its roles in the pathogenesis of type 2 diabetes.
Authors: Sepehri Z, Kiani Z, Javadian F, Akbar Nasiri A, Kohan F, Sepehrikia S, Javan Siamardi S, Aali H, Daneshvar H, Kennedy D Abstract Type 2 diabetes (T2D) is the most prevalent non—infectious disease and leads to several complications including nephropathy and retinopathy. The mechanisms and signaling molecules responsible for the development and progression of T2D, as well as its associated complications are yet to be identified. It would appear that genetic backgrounds and immunological parameters of people susceptible to T2D may play important roles in induction of T2D. TLRs participate in several...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research