Rapamycin protects against neuronal death and improves neurological function with modulation of microglia after experimental intracerebral hemorrhage in rats.
Authors: Li D, Liu F, Yang T, Jin T, Zhang H, Luo X, Wang M Abstract Intracerebral hemorrhage (ICH) results in a devastating brain disorder with high mortality and poor prognosis and effective therapeutic intervention for the disease remains a challenge at present. The present study investigated the neuroprotective effects of rapamycin on ICH-induced brain damage and the possible involvement of activated microglia. ICH was induced in rats by injection of type IV collagenase into striatum. Different dose of rapamycin was systemically administrated by intraperitoneal injection beginning at 1 h after ICH induction. We...
Source: Cellular and Molecular Biology - October 20, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Induction of apoptosis by opium in some tumor cell lines.
Authors: Khaleghi M, Farsinejad A, Dabiri S, Asadikaram G Abstract The current study is aimed at investigation of the opium effects on the apoptosis of different cell lines in culture medium and compares such effects with one another. The study is carried out on over 8 cell lines (AA8, AGS, Hela, HepG2, MCF7, N2a, PC12, WEHI). A 2.86 x 10-4 g/ml opium concentration was prepared and added to the culture medium of the cell lines for 48 hours. Cytotoxicity was tested by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. The apoptotic effect of opium on the cell lines was analyzed by Annexin-PI t...
Source: Cellular and Molecular Biology - October 20, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Trafficking mechanism of bone marrow-derived mesenchymal stem cells toward hepatocellular carcinoma HepG2 cells by modulating Endoglin, CXCR4 and TGF- β.
In this study, the role of CXCR4/CXCL12 axis and TGF-βR signaling were evaluated in the migration of MSCs toward HepG2 cells. The MSCs were incubated with SDF-1α (CXCL12), antagonists of CXCR4, TGF-βR, and co-receptor of TGF-β, (endoglin) for 48h. Then, the migration of these cells toward HepG2 cells was analyzed using in vitro migration assay. SDF-1α at a concentration of 100nM MSCs revealed the highest migration rate toward the conditioned medium (1.62 fold compared to the migration of un-treated MSCs; p
Source: Cellular and Molecular Biology - October 20, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

SiRNA/DOX lodeded chitosan based nanoparticles: Development, Characterization and in vitro evaluation on A549 lung cancer cell line.
Authors: Seifi-Najmi M, Hajivalili M, Safaralizadeh R, Sadreddini S, Esmaeili S, Razavi R, Ahmadi M, Mikaeili H, Baradaran B, Shams-Asenjan K, Yousefi M Abstract High-mobility group AT-hook2 (HMGA2), involved in epithelial mesenchymal transition (EMT) process, has a pivotal role in lung cancer metastasis. Lung cancer therapy with HMGA2 suppressing small interfering RNA (siRNA) has been introduced recently while doxorubicin (DOX) has been used as a frequent cancer chemotherapy agent. Both reagents have been faced with obstacles in clinic which make them ineffective. NanoParticles (NPs) provided a platform for effici...
Source: Cellular and Molecular Biology - October 20, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Protective role of curcumin on renal ischemia reperfusion injury via attenuating the inflammatory mediators and Caspase-3.
Authors: Liu FH, Ni WJ, Wang GK, Zhang JJ Abstract Renal ischemia/reperfusion (I/R) damage may arise due to nephron sparing surgery in patient with a solitary kidney of restricted renal parenchymas. Apoptosis, inflammation and oxidative stress play a significant role in the expansion of renal dysfunction following renal I/R. The aim of the current investigation was to particularize the potential effect of curcumin against hypoxia induced renal injury. The albino Wistar rats divided into groups and each group contains six rats. They groups are normal control; disease control; curcumin (5 mg/kg per day) and another g...
Source: Cellular and Molecular Biology - October 20, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Cell dedifferentiation, callus induction and somatic embryogenesis in Crataegus spp.
Authors: Taimori N, Kahrizi D, Abdossi V, Papzan AH Abstract The present study describes the effects of light conditions, different kinds and concentrations of auxins [Naphthylacetic acid (NAA) and dichlorophenoxyacetic acid (2,4-D)] with cytokinin (Kin) in MS medium on callus induction and embryogenesis in Crataegus pseudoheterophylla, C. aronia and C.meyeri. At first leave explants sections were cultured on different combinations of plant growth regulators in dark and light for callus initiation and light conditions to evaluation the percentage and duration of survival, callus diameter, callus fresh weight and dr...
Source: Cellular and Molecular Biology - October 20, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Marrubium vulgare ethanolic extract induces proliferation block, apoptosis, and cytoprotective autophagy in cancer cells in vitro.
Authors: Paunovic V, Kosic M, Djordjevic S, Zugic A, Djalinac N, Gasic U, Trajkovic V, Harhaji-Trajkovic J Abstract Marrubium vulgare is a European medicinal plant with numerous beneficial effects on human health. The aim of the study was to isolate the plant ethanolic extract (MVE) and to investigate its anti-melanoma and anti-glioma effects. MVE was prepared by the modified pharmacopoeial percolation method and characterized by UHPLC-LTQ OrbiTrap MS. MVE dose-dependently reduced viability of melanoma (B16) and glioma (U251) cells, but not peripheral blood mononuclear cells. It arrested cell cycle in S+G2/M phase,...
Source: Cellular and Molecular Biology - October 20, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Anti-bacterial effect of essential oil from Xanthium strumarium against shiga toxin-producing Escherichia coli.
This study aims to demonstrate in vitro efficacy of the essential oil (EO) extracted from Xanthium strumarium L. against E. coli O157:H7. Using the agar test diffusion, the effect of Xanthium strumarium L. EO (5, 10, 15, 30, 60, and 120 mg/mL) was verified at each of the four different growth phases of E. coli O157:H7. Cell counts of viable cells and colony forming unit (CFU) were determined at regular time points using Breed's method and colony counting method, respectively. No viable cell was detectable after the 1 hour-exposure to X. strumarium EO at 30, 60, and 120 mg/mL concentrations. No bacterial colony was formed a...
Source: Cellular and Molecular Biology - September 22, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Antibacterial activities of essential oils from Iranian medicinal plants on extended-spectrum β-lactamase-producing Escherichia coli.
Antibacterial activities of essential oils from Iranian medicinal plants on extended-spectrum β-lactamase-producing Escherichia coli. Cell Mol Biol (Noisy-le-grand). 2016;62(9):75-82 Authors: Sharifi-Rad J, Mnayer D, Roointan A, Shahri F, Ayatollahi SA, Sharifi-Rad M, Molaee N, Sharifi-Rad M Abstract The extended-spectrum beta-lactamase (ESBL) -producing Escherichia coli strains can lead to various infections particularly urinary tract infections. The main objective of this investigation was to evaluate the antibacterial activities of essential oils (EOs) from different Iranian medicinal plants ag...
Source: Cellular and Molecular Biology - September 22, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

How does the supernatant of Lactobacillus acidophilus affect the proliferation and differentiation activities of rat bone marrow-derived stromal cells?
In this study, we discussed the possible effects of the Lactobacillus acidophilus supernatant on proliferation and differentiation of the rBMSCs. For this aim, the cells were isolated from rat bone marrow, characterized by culturing on tissue specific differentiation media and stained. The cells (passage two) were treated with different concentrations of the L. acidophilus supernatant (0, 0.1, 0.3, 0.9, 3, 9 and 30 &mgr;l/ml) for 14 days. The proliferation and differentiation capacity of the cells were then determined by 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT assay) and tissue specific staini...
Source: Cellular and Molecular Biology - September 10, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Genetic basis of resistance waves among methicillin resistant Staphylococcus aureus isolates recovered from milk and meat products in Egypt.
Authors: Ammar AM, Attia AM, Abd El-Hamid MI, El-Shorbagy IM, Abd El-Kader SA Abstract Antimicrobial resistance of methicillin-resistant Staphylococcus aureus (MRSA) poses a serious problem for clinicians worldwide. The present study attempted to evaluate the susceptibility patterns of MRSA to various antimicrobials and the prevalence of inducible clindamycin resistance as well as the relevant antibiotic and antiseptic resistance genes among these isolates. Totally, 40 MRSA isolates were recovered from examined milk and meat product samples (18.60%). Multi-drug resistance (MDR) was remarkably observed among 85% of ...
Source: Cellular and Molecular Biology - September 10, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Modifying the mechanical properties of silk nanofiber scaffold by knitted orientation for regenerative medicine applications.
Authors: Dodel M, Hemmati Nejad N, Bahrami SH, Soleimani M, Hanaee-Ahvaz H Abstract Tissue reconstruction is among the increasing applications of polymer nanofibers. Fibrous scaffolds (mats) can be easily produced using the electrospinning method with structure and biomechanical properties similar to those of a cellular matrix. Electrospinning is widely used in the production of nanofibers and the GAP-method electrospinning is one of the means of producing fully aligned nanofibers. In this research, using the GAP-method, knitted fibrous scaffolds were made of silk fibroin, which is a biocompatible and biodegradable...
Source: Cellular and Molecular Biology - September 10, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

RNAi-induced silencing in floral tissues of Petunia hybrida by agroinfiltration: a rapid assay for chalcone isomerase gene function analysis.
Authors: Keykha F, Bagheri A, Moshtaghi N, Bahrami AR, Sharifi A Abstract Variegation in flower color is commonly observed in many plant species and also occurs on Petunia (Petunia hybrida) as an ornamental plant. Variegated plants are of highly valuable in the floricultural market. Agroinfiltration is an Agrobacterium-mediated transient assay for the analysis of gene function and genetic modification in leaves, flowers and fruit tissues of various plants. Transient RNAi-induced silencing by agroinfiltration has been developed in leaves and fruits of several plant species. Here we report the establishment of a ...
Source: Cellular and Molecular Biology - September 10, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Development of a loop-mediated isothermal amplification assay for rapid detection of Burkholderia mallei.
Authors: Mirzai S, Safi S, Mossavari N, Afshar D, Bolourchian M Abstract The present study was conducted to establish a Loop-mediated isothermal amplification (LAMP) technique for the rapid detection of B. mallei the etiologic agent of glanders, a highly contagious disease of equines. A set of six specific primers targeting integrase gene cluster were designed for the LAMP test. The reaction was optimized using different temperatures and time intervals. The specificity of the assay was evaluated using DNA from B.pseudomallei and Pseudomonas aeruginosa. The LAMP products were analyzed both visually and under UV ligh...
Source: Cellular and Molecular Biology - September 10, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Endophilin B1 regulates EGFR endocytic degradation in prostate cancer cell.
Authors: Zhu JY, Xiong Y, Zhang W, Wan J, Wan J Abstract Prostate cancer (Pca) is one of the most common types of cancer for elder men. Aberrant expression of epidermal growth factor receptor (EGFR) and EGFR downstream signaling have been known to contribute to disease progression in prostate cancer. EGF-stimulated EGFR is internalized and the process of endocytic degradation of EGFR mediates its signaling which is frequently dysregulated in many kinds of cancer. In the present study, we demonstrated that endophilin B1 expression was inhibited and EGFR expression was significantly increased in prostate cancer cell ...
Source: Cellular and Molecular Biology - September 10, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

ERBB4 gene polymorphisms and the risk of prostate cancer in a sample of Iranian Population.
Authors: Hashemi M, Moradi N, Rezaei M, Sanaei S, Ziaee SA, Narouie B, Sotoudeh M, Bahari G, Ghavami S Abstract Genetic polymorphisms in ERBB4 are thought to be associated with cancer susceptibility. In the present study, we aimed to assess the impact of ERBB4 rs12052398 T>C, rs13393577 A>G, rs13424871 A>T, rs16847082 A>G and rs6147150 (12-bp I/D) polymorphisms on risk of prostate cancer (PCa) in a sample of Iranian population. In a case-control study, we enrolled 169 patients with pathologically confirmed PCa and 182 subjects with benign prostatic hyperplasia (BPH). No significant association was found...
Source: Cellular and Molecular Biology - September 10, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Protective potency of clove oil and its transcriptional down-regulation of Aeromonas sobria virulence genes in African catfish (Clarias gariepinus L.).
Authors: Abd El-Hamid MI, Abd El-Aziz NK, Ali HA Abstract Disease episodes of fish caused by Aeromonas species are moved to the top list of limiting problems worldwide. The present study was planned to verify the in vitro antibacterial activities as well as the in vivo potential values of clove oil and ciprofloxacin against Aeromonas sobria in African catfish (Clarias gariepinus). The in vitro phenotypic virulence activities and the successful amplification of aerolysin and hemolysin genes in the precisely identified A. sobria strain were predictive for its virulence. In the in vivo assay, virulence of A. sobria st...
Source: Cellular and Molecular Biology - September 10, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Genetic diversity of multidrug resistant Staphylococcus aureus isolated from clinical and non clinical samples in Egypt.
Authors: Bendary MM, Solyman SM, Azab MM, Mahmoud NF, Hanora AM Abstract In recent years, the increasing incidence of diseases caused by Staphylococcus aureus (S. aureus) has been noted in the university hospitals of El-Sharkia and Assuit governorates - Egypt. Therefore, we studied the genetic relatedness of multidrug resistant S. aureus isolates from different sources in the above mentioned governorates. One hundred and fifty six S. aureus isolates were divided into 5 different groups, 1 non clinical isolates from different food products and 4 different clinical isolates of human and animal sources in the 2 differ...
Source: Cellular and Molecular Biology - September 10, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Three-dimensional stress In vitro promotes the proliferation and differentiation of periodontal ligament stem cells implanted by bioactive glass.
Authors: Wang T, Li G, Chen J, Lin Z, Qin H, Ji J Abstract To analyze the biological and mechanical microenvironment on the directional differentiation of periodontal ligament stem cells (PDLSCs) In vitro. PDLSCs were cultured in three-dimensional stress system In vitro for 1, 2 and 3 weeks. Methods like immunohistochemistry and flow cytometry were adopted and the proliferation and differentiation situation of PDLSCs were determined. Bioactive glass (BAG) of 0%, 10%, 20%, 30% and 40% was implanted into PDLSCs with or without three-dimensional stress for 3 weeks, respectively. The proliferation and differentiation s...
Source: Cellular and Molecular Biology - September 10, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Comparative characteristics of mesenchymal stem cells derived from reamer-irrigator-aspirator, iliac crest bone marrow, and adipose tissue.
In this study we compared MSCs derived from three different sources (iliac crest bone marrow (ICBM), adipose tissue (AT), and (RIA)) regarding the morphology, the success rate of isolating MSCs, colony frequency, expansion potential, osteogenic and chondrogenic differentiation capacity. MSCs were isolated from three different sources and flow cytometric analyses were performed for cell characterization. Colony-forming unit-fibroblast (CFU-F) assay and population doubling time (PDT) were evaluated for MSCs derived from three different sources and differentiation potential of RIA, ICBM-, and AT-MSCs were determined by staini...
Source: Cellular and Molecular Biology - September 10, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Mitosis-specific phosphorylation of PML at T409 regulates spindle checkpoint.
Authors: Jin J, Liu J Abstract During mitosis, Promyelocytic leukemia nuclear bodies (PML NBs) change dramatically in morphology and composition, but little is known about function of PML in mitosis. Here, we show that PML is phosphorylated at T409 (PML p409) in a mitosis-specific manner. More importantly, PML p409 contributes to maintain the duration of pro-metaphase and regulates spindle checkpoint. Deficient PML p409 caused a shortening of pro-metaphase and challenged the nocodazole-triggered mitotic arrest. T409A mutation led to a higher frequency of misaligned chromosomes on metaphase plate, and subsequently d...
Source: Cellular and Molecular Biology - September 10, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The antiproliferative effect of Origanum majorana on human hepatocarcinoma cell line: suppression of NF- κB.
This study aimed to investigate the antiproliferative effect of aqueous and ethanol extracts of Origanum majorana leaf on human hepatocellular carcinoma (HepG2) cell line through incubation of various concentrations of origanum majorana extracts with HepG2 and at different time intervals. The effects of aqueous and ethanol extracts of O. majorana L. on HepG2 cell viability, nuclear factor kappa B (NF-kB) gene expression were examined. The results of the cell viability assays showed that aqueous and ethanol extracts exhibited a highly significant inhibitory effect on HepG2 cell proliferation which was evidenced by a reducti...
Source: Cellular and Molecular Biology - September 10, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Spirulina exhibits hepatoprotective effects against lead induced oxidative  injury in newborn rats.
Spirulina exhibits hepatoprotective effects against lead induced oxidative injury in newborn rats. Cell Mol Biol (Noisy-le-grand). 2016;62(10):85-3 Authors: Gargouri M, Ben Saad H, Ben Amara I, Magné C, El Feki A Abstract Lead is a toxic metal that induces a wide range of biochemical and physiological effects. The present investigation was designed at evaluating the toxic effects of a prenatal exposure to lead of mothers on hepatic tissue of newborn rats, and potent protective effects of spirulina. Female rats were randomly divided into 4 groups which were given a normal diet (control),a di...
Source: Cellular and Molecular Biology - September 10, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Herbal Antibiotics: Moving back into the mainstream as an alternative for "Superbugs".
Herbal Antibiotics: Moving back into the mainstream as an alternative for "Superbugs". Cell Mol Biol (Noisy-le-grand). 2016;62(9):1-2 Authors: Sharifi-Rad J Abstract Editorial. PMID: 27585254 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - September 3, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Factors contributes to spontaneous abortion caused by Listeria monocytogenes, in Tehran, Iran, 2015.
Authors: Pourkaveh B, Ahmadi M, Eslami G, Gachkar L Abstract Spontaneous abortion is the loss of a fetus before the 20th week of pregnancy, when occurring naturally without any surgical or pharmaceutical intervention. On the other hand, Listeria monocytogenes, as one of the foodborne pathogens, is a causative agent of listeriosis. The transfer of L. monocytogenes in pregnant women occurs as self-limited flu-like symptoms which may result in abortion, stillbirth or premature birth of infected infants. The purpose of this study was the identification of Listeria monocytogenes risk factors in women with spontaneous ab...
Source: Cellular and Molecular Biology - September 3, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Potential effect of Olea europea leaves, Sonchus oleraceus leaves and Mangifera indica peel extracts on aromatase activity in human placental microsomes and CYP19A1 expression in MCF-7 cell line: Comparative study.
In this study, different plant extracts from Olea europaea leaves (OLE), Sonchus oleraceus L. (SOE) and Mangifera indica peels (MPE) were prepared to identify phytoconstituents and measure antioxidant capacities. The effects of these three extracts on aromatase activity in human placental microsomes were evaluated. Additionally, the effects of these extracts on tissue-specific promoter expression of CYP19A1 gene in cell culture model (MCF-7) were assessed using qRT-PCR. Results showed a concentration-dependent decrease in aromatase activity after treatment with OLE and MPE, whereas, SOE showed a biphasic effect. The differ...
Source: Cellular and Molecular Biology - September 3, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Tordylium persicum Boiss. & Hausskn extract: A possible alternative for treatment of pediatric infectious diseases.
Tordylium persicum Boiss. & Hausskn extract: A possible alternative for treatment of pediatric infectious diseases. Cell Mol Biol (Noisy-le-grand). 2016;62(9):20-6 Authors: Sharifi-Rad J, Fallah F, Setzer WN, Entezari Heravi R, Sharifi-Rad M Abstract Antimicrobial herbal compounds are one of the important medical resources, and in order to help alleviate the spread of the pediatric infectious diseases, identification of additional bioactive phytochemicals and herbal extracts will be practical in treating illnesses. In the present work, antimicrobial activities various extracts of Tordylium persicum...
Source: Cellular and Molecular Biology - September 3, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Potential of Ocimum basilicum L. and Salvia officinalis L. essential oils against biofilms of P. aeruginosa clinical isolates.
Authors: Stojanović-Radić Z, Pejcić M, Stojanović N, Sharifi-Rad J, Stanković N Abstract Biofilms are complex communities of microorganisms, responsible for more than 60% of the chronic human infections and they represent one of the leading concerns in medicine. Pseudomonas aeruginosa is human pathogenic bacteria which causes numerous diseases and is known for its ability to produce biofilm. Ocimum basilicum L. (basil) and Salvia officinalis L. (sage) are widely used plants in traditional medicine for the treatment of different conditions. Therefore, the aim of this study was to investigate the potential of ba...
Source: Cellular and Molecular Biology - September 3, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Association of P53 codon 72 polymorphism and lung cancer in an ethnic Iranian population.
Authors: Eydian Z, Asna'ashari AM, Behravan J, Sharifi-Rad J, Entezari Heravi R Abstract Lung cancer is one of the most common causes of cancer death worldwide. Molecular genetic studies indicated that activation of dominant oncogenes or inactivation of tumor suppressor genes and the presence of polymorphism in these genes correlated with prevalence of new lung cancers. P53 as a tumor suppressor gene located at 17p13 chromosome and it is one of the most well-known mutant genes in all cancer types. Mutation in P53 can disturb the transcriptional function and suppression of cell cycle control and increase in cell div...
Source: Cellular and Molecular Biology - September 3, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Anti-methicillin-resistant Staphylococcus aureus (MRSA) activity of Rubiaceae, Fabaceae and Poaceae plants: A search for new sources of useful alternative antibacterials against MRSA infections.
In this study, we evaluated the effects of the extracts of the leaves of species from the Rubiaceae (Galium aparine L. and Asperula arvensis L.), Fabaceae (Lathyrus aphaca L. and Vicia narbonensis L.) and Poaceae (Digitaria sanguinalis (L.) Scop. and Hordeum murinum L.) plant families on a wide and extensive panel of isolated methicillin-resistant Staphylococcus aureus strains (MRSA). The effects of the methanolic leaf extracts of Rubiaceae, Fabaceae and Poaceae plants on MRSA were evaluated by the disc diffusion assay and the broth dilution method. Among a total of 177 S. aureus isolates, 92 (51.97%) were found to be meth...
Source: Cellular and Molecular Biology - September 3, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Green synthesis of silver nanoparticles using Salvadora persica L. and its antibacterial activity.
In this study, AgNPs was synthesized using aqueous extract of Salvadora persica bark. The synthesized AgNPs were characterized by UV-Vis spectroscopy, Powder X-ray diffraction (PXRD) and Transmission electron microscopy (TEM) methods. The optimal synthesis condition to prepare nanoparticles was determined as silver nitrate 3 mM, 5 ml of aqueous extract in the room temperature for 1 h. The TEM image of AgNPs showed the formation of spherical, non-uniform nanoparticles of mean size of 50 nm. The antibacterial activity of synthesized AgNPs was evaluated using disk diffusion, minimum inhibitory concentration (MIC) and minimum ...
Source: Cellular and Molecular Biology - September 3, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

A comparison between PLGA-PEG and NIPAAm-MAA nanocarriers in curcumin delivery for hTERT silencing in lung cancer cell line.
Authors: Roointan A, Sharifi-Rad M, Badrzadeh F, Sharifi-Rad J Abstract Lung cancer is the most common cancer among men. Since the main reason of cancer cells immortality is telomerase activity, targeting of such enzyme can be a promising approach in cancer therapy. Curcumin is a safe and efficient anticancer agent in this context, but its applications in cancer therapy are limited because of its hydrophobic structure and low solubility in water. Today, using nanocarriers for delivery of such anticancer agents is a well performed method. Here, we developed and compared the efficiency of two nanocarriers (PLGA-PEG a...
Source: Cellular and Molecular Biology - September 3, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Plants of the genus Allium as antibacterial agents: From tradition to pharmacy.
Authors: Sharifi-Rad J, Mnayer D, Tabanelli G, Stojanović-Radić ZZ, Sharifi-Rad M, Yousaf Z, Vallone L, Setzer WN, Iriti M Abstract Plants belonging to the genus Allium are widely cultivated and used all over the world as food and medicinal plants. Since ancient times, these plants, particularly garlic (Allium sativum L.) and onion (Allium cepa L.), have represented important components of typical recipes and traditional healing systems. Not the least of which, their use as food biopreservatives is well documented, due to the relevant antibacterial activity of their extracts and essential oils. In addition to gar...
Source: Cellular and Molecular Biology - September 3, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Methicillin-Resistant Staphylococcus Aureus infection exacerbates NSCLC cell metastasis by up-regulating TLR4/MyD88 pathway.
In conclusion, MRSA infection can enhance NSCLC cell metastasis by up-regulating TLR4/MyD88 signaling. PMID: 27545207 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - August 24, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Helicobacter pylori infection and iron deficiency in patients with coronary artery disease.
Authors: Fallah S, Ahmadi R, Moradi N, Fadaei R, Sezavar SH, Seifi M Abstract The aim of this study was to investigate whether impact of the seropositivity to Helicobacter pylori (H pylori) infection on ferritin and iron levels is an independent risk factor for atherosclerosis in patients with cardiovascular disease. The anti H pylori IgG, IgA levels, serum ferritin and iron concentration of 86 patients with cardiovascular disease and 64 participants free of cardiovascular disease as control subjects were determined by ELISA assay. The results of present study showed that seropositivity to H pylori IgG and IgA leve...
Source: Cellular and Molecular Biology - August 24, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Strain differentiation of Mycobacterium tuberculosis for epidemiology in northwest of Iran.
In this study, 194 positive cultures of M. tuberculosis in North West Iran were evaluated by the MIRU-VNTR method. MIRU-12 differentiated the 194 isolates into 138 different patterns, comprising 30 clusters and 108 unique patterns (HGDI=0.9930). The largest cluster contained twelve isolates. The results showed that the majority of TB cases in North West Iran are due to reactivation and the 12-MIRU typing method can be used as a first-line method for strain differentiation of M. tuberculosis. PMID: 27545209 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - August 24, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Preliminary characterization of human skin microbiome in healthy Egyptian individuals.
This study presents a new insight for subsequent analyses of human microbiome in Egypt. PMID: 27545210 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - August 24, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Hemodynamic shear stress regulates the transcriptional expression of heparan sulfate proteoglycans in human umbilical vein endothelial cell.
Authors: Liu JX, Yan ZP, Zhang YY, Wu J, Liu XH, Zeng Y Abstract We have previously demonstrated the adaptive remodeling of endothelial glycocalyx under shear stress. However, the underlying mechanism in glycocalyx remodeling, especially the expression of the components heparan sulfate proteoglycans (HSPGs) under shear stress was not completely known. In the present study, we investigated the expression of those HSPGs (syndecan family and glypican-1) in human umbilical vein endothelial cells (HUVECs) responded to the distinct magnitude of shear stress, and performed a systematic and comprehensive analyze on the rel...
Source: Cellular and Molecular Biology - August 24, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Can rs3767140 SNP of the perlecan (HSPG2) gene affect the diabetes mellitus through the dyslipidemia?
Authors: Kurnaz-Gömleksiz Ö, Tokat B, Aslan EI, Yanar F, Ermiş-Karaali Z, Öztürk O, Yilmaz-Aydoğan H Abstract Perlecan (HSPG2) play an important role in the lipoprotein metabolisms. The G allele of the HSPG2-rs3767140 may affect the binding of heparan sulfate (HS) chains and hence cause loss of HS from the basement membrane. HSPG2-rs3767140 was studied in 60 T2DM patients and 109 healthy controls. In diabetic patients HSPG2-rs3767140 T variant allele carriers (TT+GT) have decreased fasting plasma glucose (FPG) and serum LDL-C levels (p=0.071 and p=0.060, respectively) versus GG genotype carrie...
Source: Cellular and Molecular Biology - August 24, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Irisin immunostaining characteristics of breast and ovarian cancer cells.
Authors: Kuloglu T, Celik O, Aydin S, Hanifi Ozercan I, Acet M, Aydin Y, Artas G, Turk A, Yardim M, Ozan G, Hanifi Yalcin M, Kocaman N Abstract To determine expression pattern of irisin in tissues obtained from human ovarian cancer, breast cancer, and cervix cancer. Tissue samples obtained from subjects with breast cancer, ovarian cancer cervix cancer, simple endometrial hyperplasia, complex atypical endometrial hyperplasia. At least five sections from each subject were immunohistochemically stained with irisin antibody, and H-score method was used to evaluate irisin intensity. Tissues obtained from healthy breast ...
Source: Cellular and Molecular Biology - August 24, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Cloning and expression of recombinant human platelet-derived growth factor-BB in Pichia Pink.
Authors: Babavalian H, Latifi AM, Shokrgozar MA, Bonakdar S, Tebyanian H, Shakeri F Abstract The PDGF-BB plays a key role in several pathogenesis diseases and it is believed to be an important mediator for wound healing. The recombinant human PDGF-BB is safe and effective to stimulate the healing of chronic, full thickness and lower extremity diabetic neurotrophic ulcers. In the present study, we attempted to produce a PDGF-BB growth factor and also, evaluate its functionality in cell proliferation in yeast host Pichia pink. Pichia pink yeast was used as a host for evaluation of the rhPDGF-BB expression. The coding...
Source: Cellular and Molecular Biology - August 24, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

PPAR-gamma in overcoming kinase resistance in chronic myeloid leukemia.
Authors: Yousefi B, Shafiei-Irannejad V, Azimi A, Samadi N, Zarghami N Abstract Peroxisome proliferator-activated receptor gamma (PPARγ) plays key roles in regulating cellular differentiation, proliferation and apoptosis pathways. As such, they are considered promising targets for anticancer drug development, especially for breast cancer, multiple myeloma and hematologic malignancies. Chronic myeloid leukemia (CML) is a myeloproliferative disorder arising from an oncogenic Bcr-Abl tyrosine kinase. Inhibitors of this oncogene by small molecules such as imatinib are effective only in 75% of the patient's popula...
Source: Cellular and Molecular Biology - August 24, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Lanthanum chloride enhances cisplatin-induced apoptosis in ovarian cancer cells.
In this study, we investigated the influence of LaCl3 on cisplatin-induced apoptosis in the ovarian cell line (COC1). Cell-based assays and analysis of cellular ultra structure were used. Result showed that LaCl3 enhanced cisplatin-induced apoptosis in COC1. Furthermore, the application of Cisplatin also altered the expression level of tumor related proteins, such as ERCC1, Ki67, and CDK6. Overall, we determined the inhibitory effect of LaCl3 on the COC1 growth when treated with Cisplatin, which may serve as a basis for the clinical application of LaCl3 in the treatment of ovarian cancer. PMID: 27453264 [PubMed - in p...
Source: Cellular and Molecular Biology - July 29, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Synthesis, SAR and biological evaluation of a novel series of 1-(2-chloroethyl)-1-nitroso-3-(2-(3-oxobenzoelenazol-2(3H)-yl)ethyl) urea: Organoselenium compounds for cancer therapy.
Authors: Ye S, Zheng X, Hu T, Zeng H Abstract Thioredoxin reductase 1 (TrxR1) is an important potential anticancer drug target and closely related to both carcinogenesis and cancer progression. Ethaselen (BBSKE), a novel organoselenium compound inhibiting TrxR1 with selective antitumor effect, while its symmetrical structure results in poor solubility. Carmustine (BCNU), a DNA cross-link agent and also a deactivator of TrxR, is with high toxicity and low selectivity which limit its clinical application to some extents. Herein, a novel compound, 1-(2-chloroethyl)-1-nitroso-3-(2-(3-oxobenzoel...
Source: Cellular and Molecular Biology - July 29, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effect of FUT3 gene silencing with miRNA on proliferation, invasion and migration abilities of human KATO-III gastric cancer cell line.
This study investigated the effects of FUT3 gene expression inhibition with miRNA on the proliferation, invasion and migration abilities of KATO-III cells. KATO-III cells were transfected with plasmid pcDNA™6.2-GW/EmGFP-FUT3-miR(FUT3-miRNA) and negative control plasmid in mediation of liposome, respectively, using untransfected cells as blank controls. Forty-eight hours after transfection, FUT3 mRNA levels were tested by RT-PCR. Levels of sLeA proteins were assayed by Western blot. The effects of FUT3-miRNA on the proliferation, invasion and migration of KATO-III cells were determined by CCK8 testing and Transwell as...
Source: Cellular and Molecular Biology - July 29, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

A new neurotoxicity model composed of a neurovascular unit in vitro.
In conclusion, the two models replicated the blood-brain barrier (BBB) more accurately than the control, so it has potential usefulness in further scientific and clinical drug research. Furthermore, NVU model could be used to screen neurotoxicity chemicals due to its NVU properties. PMID: 27453267 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - July 29, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Toxicity of Hoechst 33342: implication in side population analysis.
Authors: Zheng J, Qi S, Zhu H, Xiao X Abstract In the current study, we discovered that Hoechst 33342 (H342) could produce significant toxicity to the human neuroblastoma SK-N-SH cells at concentrations typically used to identify side population (SP) cells. At 3.75 and 5 µg/ml, H342 induced cell death and apoptosis. Flow cytometry analysis revealed cell cycle arrest at S-phase. Our findings caution against using H342 for SP analysis without considering differential susceptibility of different cells to H342. Specifically, concentration of H342 needs to be adjusted carefully to minimize toxicity for each cell l...
Source: Cellular and Molecular Biology - July 29, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Combined PCR and Q-RT-PCR technique for detecting chimerism in a non-human Primate vascularized osteomyocutaneous allografts model.
Authors: Jiang J, Wang J, Zhong F, Chen G, Li Y, Zheng XX Abstract Face transplantation and other composite tissue transplantation (CTA) are permissive to transplantation tolerance. The real reason, that composite tissue containing bone achieves transplantation immune tolerance more easily than the composite tissue without the bone is not clear. The chimerism may be the main mechanism in the progress of inducing the transplantation tolerance by CTA. We currently have established a non-human Primate Vascularized Osteomyocutaneous Allografts Model. To test the chimerism which comes from donor after the transplan...
Source: Cellular and Molecular Biology - July 29, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The role of apoptosis inducing factor in the apoptosis of retinal pigment epithelium cells induced by oxidative stress.
Authors: Fu Y, Tang M, Bai L, Fan Y Abstract To explore the role of apoptosis inducing factor (AIF) in the apoptosis of retinal pigment epithelium (RPE) cells induced by oxidative stress. RPE cell apoptotic models were constructed by H2O2, Annexin V/PI flow cytometry and MTT assay were used to determine the changes of RPE cell apoptosis and proliferation. The production of reactive oxygen species (ROS) and mitochondrial membrane potential were observed. QRT-PCR and western blot were used to determine the expression of AIF to evaluate whether AIF-mediated non caspase pathway participated in the RPE cell apoptosis in...
Source: Cellular and Molecular Biology - July 29, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Association between miR-499 rs3746444 and the susceptibility of hepatocellular carcinoma.
This study suggested that miR-499 rs3746444 might play an important role in the development of HCC. PMID: 27453271 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - July 29, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research