Homocysteine modulates CXCL10/CXCR3 axis activity to induce endothelial dysfunction
In conclusion, our findings illuminated the central role of the CXCL10/CXCR3 axis in mediating Hcy-induced endothelial dysfunction, providing valuable insights for potential therapeutic strategies in managing HHcy-related cardiovascular diseases.PMID:38430022 | DOI:10.14715/cmb/2024.70.2.28 (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - March 2, 2024 Category: Molecular Biology Authors: Yanjie Xu Yingying Xu Zuozong Yu Dingwen He Source Type: research
Mitochondria-targeted reactive oxygen species blockor SS-31 blocks hepatic stellate cell activation and alleviates hepatic fibrosis by regulating NLRP3 inflammasomes
This study aimed to elucidate the effect of mitochondria-targeted reactive oxygen species (ROS) blockor SS-31 on hepatic stellate cells (HSC) activation during liver fibrosis. TGF-β1 was employed to induce HSC activation, while MitoSOX Red was utilized to assess the presence of mitochondrial ROS. The mitochondrial membrane potential (MMP) was measured using the JC-1 probe, and the ATP level was determined using a specific kit. The proliferation of HSCs was assessed using CCK-8 and colony formation assays, whereas flow cytometry was employed to detect HSC apoptosis. Fibrotic markers (COL1A1 and α-SMA) and NLRP3 inflammaso...
Source: Cellular and Molecular Biology - March 2, 2024 Category: Molecular Biology Authors: Li Liu YingFeng Wei NingSheng Xie Hong Cai Ye Lin Source Type: research
Tetrandrine alleviates inflammation and promotes macrophage M2 polarization in gouty arthritis by NF- κB-mediated Lcp1
Cell Mol Biol (Noisy-le-grand). 2024 Feb 29;70(2):205-211. doi: 10.14715/cmb/2024.70.2.29.ABSTRACTGouty arthritis (GA) is an inflammatory disease caused by the deposition of monosodium urate (MSU) crystals into joints. Tetrandrine (TET) is a bisbenzylisoquinoline alkaloid extracted from the root of Stephania tetrandra and can exert an anti-inflammatory function in different diseases. Nevertheless, the specific function of TET in GA remains unclear. We established the GA mouse model by MSU injection into joints of mice. Paw volume and gait score were detected for measuring the degree of joint swelling and the situation of j...
Source: Cellular and Molecular Biology - March 2, 2024 Category: Molecular Biology Authors: Li Fang Rong Shen Yao Lu Xiangfeng Xu Fang Huang Source Type: research
Combination therapy application of Abemaciclib with Doxorubicin in triple negative breast cancer cell line MDA-MB-231
Cell Mol Biol (Noisy-le-grand). 2024 Feb 29;70(2):169-177. doi: 10.14715/cmb/2024.70.2.24.ABSTRACTDue to lack of clinical biomarkers, Triple Negative Breast Cancer (TNBC) is more likely to have spread to other tissues at time of diagnosis and therapy planning generally involves use of cytotoxic chemotherapy agents, such as Doxorubicin. We aimed to investigate possible advantages of using combination strategy using Doxorubicin alongside Abemaciclib. After determining the IC50 values for Doxorubicin (DOX) and Abemaciclib (ABE); CompuSyn and ComBenefit software were used to reveal the effect resulting from the combination of ...
Source: Cellular and Molecular Biology - March 2, 2024 Category: Molecular Biology Authors: Tugce Nur Eralp Akin Sevinc Banu Mansuroglu Source Type: research
CircSCUBE3 promoted ferroptosis to inhibit lung adenocarcinoma progression
In conclusion, circSCUBE3 inhibited LUAD development by promoting ferroptosis via the CREB/GPX4/GSH axis, which might provide a novel option for the LUAD targeted therapy.PMID:38430026 | DOI:10.14715/cmb/2024.70.2.23 (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - March 2, 2024 Category: Molecular Biology Authors: Hongye Fu Qiong Zhao Source Type: research
Effect of Liraglutide combined with Jinlida granules on glycolipid metabolism and islet function of type 2 diabetes mellitus
Cell Mol Biol (Noisy-le-grand). 2024 Feb 29;70(2):156-160. doi: 10.14715/cmb/2024.70.2.22.ABSTRACTTo investigate whether Liraglutide combined with Jinlida granules affects glycolipid metabolism and islet function in the treatment of type 2 diabetes mellitus (T2DM), a control group and an observation group were established with 90 T2DM patients. The control group was given Jinlida treatment and the observation group was given liraglutide combined treatment for 12 weeks. The clinical efficacy, glycolipid metabolism, bone metabolism, islet function, and endothelial function. The curative effect of the observation group was be...
Source: Cellular and Molecular Biology - March 2, 2024 Category: Molecular Biology Authors: Xianjuan Wang Kun He Jing Li Ying Liu Zhaokang Peng Lijuan Zhao Shumin Ji Source Type: research
Declined circular RNA mitofusin 2 constrains the deterioration of Wilms tumor via modulating microRNA-372-3p/transforming growth factor- β receptor type 2 axis
Cell Mol Biol (Noisy-le-grand). 2024 Feb 29;70(2):143-149. doi: 10.14715/cmb/2024.70.2.20.ABSTRACTTo explore the action and mechanism in which circular RNA (circRNA) mitofusin 2 (MFN2) repressed the malignant proliferation of Wilms tumor (WT) via modulating microRNA (miR)-372-3p/transforming growth factor-β receptor type 2 (TGFBR2) axis. CircRNA MFN2 was distinctly elevated in the tissues and cells of WT patients, while miR-372-3p was silenced in the tissues and cells of WT. Test of TGFBR2, PCNA and Bax was implemented. Transfection with si-circRNA MFN2 or miR-372-3p-mimic restrained cancer cell advancement and the number...
Source: Cellular and Molecular Biology - March 2, 2024 Category: Molecular Biology Authors: Maolin Xiao Xiaofeng Pu Wei Tong Xiao Xiao Source Type: research
Circ-LDLRAD3/miR-655-3p/MAPK1 axis enhances cell migration and invasion in papillary thyroid carcinoma
In conclusion, circ-LDLRAD3 stimulates PTC development by releasing miR-655-3p-targeted MAPK1.PMID:38430029 | DOI:10.14715/cmb/2024.70.2.21 (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - March 2, 2024 Category: Molecular Biology Authors: Bingfei Zhang Yiquan Peng Lirong Zhou Source Type: research
Determination of volatile aroma composition of some hazelnut varieties grown in Ak çakoca conditions
This study has shown that the climate and soil conditions of Akçakoca cause the emergence of unique taste profiles through the effect of hazelnut varieties on volatile aroma components. The findings emphasize that regional diversity and local ecosystem factors play a critical role in determining flavor in hazelnut kernels. This study also emphasizes that variety selection is a critical factor in ensuring sustainability in hazelnut cultivation and that determining volatile aroma components is an important indicator in this selection.PMID:38430030 | DOI:10.14715/cmb/2024.70.2.19 (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - March 2, 2024 Category: Molecular Biology Authors: Hulya Unver Yalcin Gucer Ozan Haliscelik Ebru Sakar Zeynep Mujde Sakar Masoumeh Khanahmadi Sezai Ercisli Source Type: research
Astragaloside IV inhibits idiopathic pulmonary fibrosis through activation of autophagy by miR-21-mediated PTEN/PI3K/AKT/mTOR pathway
Cell Mol Biol (Noisy-le-grand). 2024 Feb 29;70(2):128-136. doi: 10.14715/cmb/2024.70.2.18.ABSTRACTAs the main active ingredient of Astragalus, Astragaloside IV (AS-IV) can ameliorate pulmonary fibrosis. In this experiment, we studied how AS-IV reduces idiopathic pulmonary fibrosis (IPF). Bleomycin (BLM) or TGF-β1 was treated in mice or alveolar epithelial cells to mimic IPF in vivo as well as in vitro. ASV-IV alleviated levels of inflammatory cytokines and fibrosis markers in IPF model. Through detection of autophagy-related genes, ASV-IV was observed to induce autophagy in IPF. Besides, ASV-IV inhibited miR-21 expression...
Source: Cellular and Molecular Biology - March 2, 2024 Category: Molecular Biology Authors: Tiaohao Li Xiaojuan Gao Rui Jia Yanling Sun Yeli Ding Fei Wang Yujuan Wang Source Type: research
Synergistic anti-tumor effects of lenalidomide and gefitinib by upregulating ADRB2 and inactivating the mTOR/PI3K/AKT signaling pathway in lung adenocarcinoma
In conclusion, lenalidomide and gefitinib synergistically suppressed LUAD progression and attenuated gefitinib resistance by upregulating ADRB2 and inactivating the mTOR/PI3K/AKT signaling pathway in lung adenocarcinoma.PMID:38430032 | DOI:10.14715/cmb/2024.70.2.17 (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - March 2, 2024 Category: Molecular Biology Authors: Shuangshuang Zhou Meiyi Deng Xuyu Bian Jindong Shi Rongrui Liang Min Tao Source Type: research
Circular RNA circ_0096041 promotes osteosarcoma cell proliferation and migration via sponging miR-556-5p and regulating LIN28A expression
Cell Mol Biol (Noisy-le-grand). 2024 Feb 29;70(2):113-119. doi: 10.14715/cmb/2024.70.2.16.ABSTRACTStrategies targeting lin-28 homolog A (LIN28A) for the treatment of osteosarcoma are limited, even though salient findings have illustrated the crucial role of LIN28A in bone deformities and cancer. In the present study, we proved circ_0096041, one of the circular RNAs (circRNAs) with significant upregulated expression in osteosarcoma, to be notably engaged in the progression of osteosarcoma. We elucidated that osteosarcoma patients with highly expressed circ_0096041 had relatively worse prognoses. We determined that circ_0096...
Source: Cellular and Molecular Biology - March 2, 2024 Category: Molecular Biology Authors: Fulin Zhou Hao Wu Zhen Yin Weibo Zhou Wei Chen Source Type: research
Bone mesenchymal stem cell-derived exosomal miR-26a-3p promotes autophagy to attenuate LPS-induced apoptosis and inflammation in pulmonary microvascular endothelial cells
This study aimed to explore the treatment effect and regulatory mechanism of bone mesenchymal stem cell-derived exosomes (BMSC-expos) on ALI. PMVECs were stimulated by Lipopolysaccharide (LPS) to imitate ALI environment. Cell viability was determined by CCK-8 assay. Cell apoptosis was evaluated by TUNEL and flow cytometry. ELISA was utilized for testing the contents of TNF-α, IL-1β, IL-6, and IL-17. Western blot was applied for testing the levels of autophagy-related proteins LC3, p62, and Beclin-1. RNA interaction was determined by luciferase reporter assay. The ALI rat model was established by intratracheal injection o...
Source: Cellular and Molecular Biology - March 2, 2024 Category: Molecular Biology Authors: Qianfei Wang Yiming Huang Zherui Fu Source Type: research
miR-378a-5p represses Barrett's esophagus cells proliferation, migration and invasion through targeting TSPAN8
Cell Mol Biol (Noisy-le-grand). 2024 Feb 29;70(2):97-103. doi: 10.14715/cmb/2024.70.2.14.ABSTRACTBarrett's esophagus (BE) belongs to a pathological phenomenon occurring in the esophagus, this paper intended to unveil the underlying function of miR-378a-5p and its target TSPAN8 in BE progression. GEO analysis was conducted to determine differentially expressed genes in BE samples. Non-dysplastic metaplasia BE samples, high-grade dysplastic BE samples and controls were collected from subjects. CP-A and CP-B cells were exposed to bile acids (BA) to mimic gastroesophageal reflux in BE cells. RT-qPCR as well as western blot wer...
Source: Cellular and Molecular Biology - March 2, 2024 Category: Molecular Biology Authors: Xudong Xie Duo Wei Source Type: research
Green synthesis and characterization of silver nanoparticles from Ducrosia flabellifolia Boiss. aqueous extract: Anti-quorum sensing screening and antimicrobial activities against ESKAPE pathogens
Cell Mol Biol (Noisy-le-grand). 2024 Feb 29;70(2):88-96. doi: 10.14715/cmb/2024.70.2.13.ABSTRACTBiosynthesis of silver nanoparticles using natural compounds derived from plant kingdom is currently used as safe and low-cost technique for nanoparticles synthesis with important abilities to inhibit multidrug resistant microorganisms (MDR). ESKAPE pathogens, especially MDR ones, are widely spread in hospital and intensive care units. In the present study, AgNPs using Ducrosia flabellifolia aqueous extract were synthesized using a reduction method. The green synthesized D. flabellifolia-AgNPs were characterized by UV-Vis spectr...
Source: Cellular and Molecular Biology - March 2, 2024 Category: Molecular Biology Authors: Mejdi Snoussi Ramzi Hadj Lajimi Salman Latif Walid Sabri Hamadou Mousa Alreshidi Syed Amir Ashraf Mitesh Patel Jamal R Humaidi El Hassane Anouar Adel Kadri Emira Noumi Source Type: research
