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Long noncoding RNA HOTTIP contributes to the progression of prostate cancer by regulating HOXA13.
In conclusion, down-regulation of HOTTIP and HOXA13 was associated with cell growth and cell cycle, and exerts tumor-suppressive functions in the genesis and progression of prostate cancer, providing a potential attractive therapeutic approach for this malignancy. PMID: 27064878 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - April 13, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Association between herbivore stress and glutathione S-transferase expression in Pinus brutia Ten.
In this study, we investigated the changes in activity and mRNA expression of GST in pine samples taken from both resistant and susceptible clones against T. wilkinsoni over a nine month period in a clonal seed orchard. It was found that the average cytosolic GST activities of trees in March and July were significantly higher than the values obtained in November. November was considered to be the control since trees were not under stress yet. In addition, RT-PCR results clearly showed that levels of GST transcripts in March and July samples were significantly higher as compared to the level seen in November. These findings...
Source: Cellular and Molecular Biology - April 13, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Role of microRNA-129-5p in osteoblast differentiation from bone marrow mesenchymal stem cells.
Authors: Xiao WZ, Gu XC, Hu B, Liu XW, Zi Y, Li M Abstract Mesenchymal stem cells derived from bone marrow have the capacity to differentiate into osteoblast, chondrocyte, nerve cell and myocardial cell in vitro, which are an ideal engraft in tissue-engineered repair. Osteoblast differentiation is a vital process in maintaining bone homeostasis in which various transcriptional factors, including signaling molecules, and microRNAs (miRNAs). In this research, human bone marrow mesenchymal stem cells (hBMSCs) were induced differentiation into osteoblast in vitro after over-expression of miR-129-5p. The results showed ...
Source: Cellular and Molecular Biology - April 13, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Different ideas associated renal malformation and laminin α5 expression caused by maternal nicotine exposures.
In this study, the effects of maternal nicotine exposure on expression levels of kidney laminin α5 in newborn mice were examined. Timed pregnant mice were injected subcutaneously with nicotine at a dose of 2 mg/kg/day from day 7 of gestation to the last day of the pregnancy (Group 1) and from day 7 until the two weeks of postnatal (Group 2). Sham control groups were injected with saline. After the last injection, all the newborn mice were anesthetized; their kidneys were removed and prepared for analysis of mRNA and protein expression of laminin α5 using Real-Time PCR and immunohistochemical techniques, respect...
Source: Cellular and Molecular Biology - April 13, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Hypoxia stimulates the proliferation of rat neural stem cells by regulating the expression of metabotropic glutamate receptors: an in vitro study.
Authors: Chen X, Liu Y, Zhang Z, Miao X, Tian Y, Yao L, Liu J, Lu H, Liu Y Abstract Neural stem cells (NSCs) reside in not only developing, but also adult brain with specialized microenvironments that regulate their function. In vitro and in vivo studies have revealed strong regulatory links between hypoxic/ischemic insults and activation of NSCs. However, the underlying mechanisms remain unclear. Here, we show that proliferating NSCs isolated from rat E15.5 cortex expressed functional metabotropic glutamate receptor (mGluR) subtype 3-7. Hypoxic exposure regulated their expression in NSCs in mRNA and protein levels...
Source: Cellular and Molecular Biology - April 13, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Cavernosum smooth muscle relaxation induced by Schisandrol A via the NO-cGMP signaling pathway.
Authors: Liu W, Choi BR, Bak YO, Zhang LT, Zhou LX, Huang YR, Zhao C, Park JK Abstract To evaluate the effect of Schisandrol A on rabbit corpus cavernosum smooth muscle and elucidate the potential mechanism. Penises were obtained from healthy male New Zealand White rabbits (2.5-3.0 kg). The pre-contracted penis with phenylephrine (Phe, 10 µM) was treated with accumulative concentrations of Schisandrol A (10-7, 10-6, 10-5 and 10-4 M). The change in intracavernosum pressure (ICP) and tension was recorded, cyclic nucleotides in the cavernosum tissue were measured by radioimmunoassay, mRNA level and expression of...
Source: Cellular and Molecular Biology - April 13, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

A comparative evaluation of four DNA extraction protocols from whole blood sample.
Authors: Ghaheri M, Kahrizi D, Yari K, Babaie A, Suthar RS, Kazemi E Abstract All organisms have Deoxyribonucleic acid (DNA) within their cells. DNA is a complex molecule that contains all of the information necessary to build and maintain an organism. DNA extraction is one of the most basic and essential techniques in the study of DNA that allow huge advances in molecular biology, biotechnology and bioinformatics laboratories. Whole blood samples are one of the main sources used to obtain DNA and there are many different protocols available in this issue. In current research, compared four DNA extraction protocols...
Source: Cellular and Molecular Biology - April 13, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Sox9-induced chondrogenesis in mesenchymal stem cells was mediated by ERK5 signal pathway.
This study will help to understand the interaction between the ERK5 and Sox9, and facilitate to decipher the mechanism of chondrogenesis in mesenchymal stem cells. PMID: 26950443 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - March 9, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Semaphorin 3A deficiency improves hypoxia-induced myocardial injury via resisting inflammation and cardiomyocytes apoptosis.
In this study, decline in Sema 3A was discovered in hypoxia-treated myocardial cells. When this decline was enhanced by silencing of Sema 3A gene, hypoxia-induced myocardial cell injury could be partially improved. Sema 3A deficiency can resist hypoxia-induced inflammatory factors (TNF-α, IL-1β and IL-6) secretion, cell viability decline, cardiomyocyte apoptosis, ROS release, ATP generation decline as well as GSH/GSSG ratio decline in H9C2 cells. Besides, hypoxia-induced bcl-2 decrease and cleaved caspase-3 increase also can be partially reversed during Sema 3A deficiency. All these findings reflect that reduced...
Source: Cellular and Molecular Biology - March 9, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Genetic analysis of Iranian autosomal dominant polycystic kidney disease: new insight to haplotype analysis.
Authors: Entezam M, Khatami MR, Saddadi F, Ayati M, Roozbeh J, Saghafi H, Keramatipour M Abstract Autosomal Dominant Polycystic Kidney Disease (ADPKD) caused by mutations in two PKD1 and PKD2 genes. Due to the complexity of the PKD1 gene, its direct mutation screening is an expensive and time-consuming procedure. Pedigree-based haplotype analysis is a useful indirect approach to identify the responsible gene in families with multiple affected individuals, before direct mutation analysis. Here, we applied this approach to investigate 15 appropriate unrelated ADPKD families, selected from 25 families, who referred fo...
Source: Cellular and Molecular Biology - March 9, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Evaluation of MMP-7 A-181G and MMP-2 C-735T polymorphisms in healthy population from western Iran.
Authors: Rahimi Z, Yari K, Rahimi Z Abstract Matrix metalloproteinases (MMPs) are involved in multiple physiological and pathological processes. Variable frequency of the MMPs gene variants might affect the susceptibility to certain diseases. The aim of present study was to investigate the frequency of MMP-7 A-181G and MMP-2 C-735T variants in healthy population of Western Iran with Kurdish ethnic background. Individuals were medical students and staff members of the Medical School of Kermanshah University and blood donors that consisted of 221 females and 94 males. Control subjects were free of general and genetic...
Source: Cellular and Molecular Biology - March 9, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Differential actıvation and expression of insulin receptor substrate 1 (IRS1) in mononuclear cells of type 2 diabetes patients after insulin stimulation.
Authors: Gorgisen G, Balci MK, Celik FC, Gokkaya M, Ozdem S, Ozel D, Ozes ON Abstract Insulin regulates the glucose homeostasis by inducing tyrosine phosphorylation of insulin receptor substrate (IRS) proteins. IRS1 is the best studied member of this family and insulin-induced Tyrosine phosphorylation of (YXXM) motifs provides docking site for SH2 domain-containing proteins. Recent studies have suggested that genetic and/or environmental factors may affect the expression and phosphorylation levels of IRS1, and these could be important for development of insulin resistance. To shed light to the molecular basis of ty...
Source: Cellular and Molecular Biology - March 9, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

microRNA-23a-5p acts as a potential biomarker for sepsis-induced acute respiratory distress syndrome in early stage.
In conclusion, the miR-23a-5p might be employed as the potential biomarkers for ARDS in early stage. PMID: 26950448 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - March 9, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Isoflurane preconditioning protects rat brain from ischemia reperfusion injury via up-regulating the HIF-1α expression through Akt/mTOR/s6K activation.
Isoflurane preconditioning protects rat brain from ischemia reperfusion injury via up-regulating the HIF-1α expression through Akt/mTOR/s6K activation. Cell Mol Biol (Noisy-le-grand). 2016;62(2):38-44 Authors: Yan W, Chen Z, Chen J, Chen H Abstract The volatile anesthetic isoflurane (ISO) has been widely used in ischemia reperfusion (IR) injury because its abilities to induce and trigger recovery are faster and smoother than other agents. However, the underlying molecular mechanisms for preconditioning the ISO in protecting the brain against IR injury are still largely unclear. In this paper, we ...
Source: Cellular and Molecular Biology - March 9, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Association of SHMT1 gene polymorphisms with the risk of childhood acute lymphoblastic leukemia in a sample of Iranian population.
Authors: Bahari G, Hashemi M, Naderi M, Sadeghi-Bojd S, Taheri M Abstract The enzymes serine hydroxymethyltransferase 1 (SHMT1) regulate key reaction in folate-mediated one-carbon metabolism. In the current study we aimed to examine the possible association between SHMT1 gene polymorphisms and childhood acute lymphoblastic leukemia (ALL) in a sample of Iranian population. The rs9901160, rs2273027, rs9909104, rs1979277, and rs11868708 gene polymorphisms of SHMT1 were genotyped in 120 children diagnosed with ALL and 120 healthy children by the polymerase chain reaction restriction fragment length polymorphism (PCR-RF...
Source: Cellular and Molecular Biology - March 9, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Molecular versus conventional culture for detection of respiratory bacterial pathogens in poultry.
Authors: Ammar AM, Abd El-Aziz NK, Abd El Wanis S, Bakry NR Abstract Acute respiratory tract infections are leading causes of morbidity in poultry farms allover the world. Six pathogens; Escherichia coli, Mycoplasma gallisepticum, Staphylococcus aureus, Pasteurella multocida, Mannheimia haemolytica and Pseudomonas aeruginosa were involved in respiratory infections in poultry. Herein, conventional identification procedures and polymerase chain reaction (PCR) were applied for detection of the most common respiratory bacterial pathogens in clinical specimens of poultry obtained from 53 Egyptian farms with various resp...
Source: Cellular and Molecular Biology - March 9, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Ghrelin inhibits oxLDL-induced inflammation in RAW264.7 mouse macrophages through down-regulation of LOX-1 expression via NF-κB signaling pathway.
Ghrelin inhibits oxLDL-induced inflammation in RAW264.7 mouse macrophages through down-regulation of LOX-1 expression via NF-κB signaling pathway. Cell Mol Biol (Noisy-le-grand). 2016;62(2):57-61 Authors: Sun N, Wang H, Wang L Abstract Oxidized low-density lipoprotein (oxLDL) is one of the many causes of the initiation and progression of atherosclerosis, which can subsequently promote the uptake of oxLDL by macrophages and lead to inflammation in the blood vessels. In the present study, we evaluated the protective effects of ghrelin on oxLDL-induced RAW264.7 mouse macrophages. Ghrelin was able to...
Source: Cellular and Molecular Biology - March 9, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effects of Urtica dioica dichloromethane extract on cell apoptosis and related gene expression in human breast cancer cell line (MDA-MB-468).
This study is the first to examine the cytotoxic and apoptotic effects of Urtica dioica in MDA-MB-468, human breast adenocarcinoma cells. The 3-(4,5-dimethylethiazol-2 yl)-2,5- diphenyltetrazolium (MTT) reduction and trypan-blue exclusion assay were performed in MDA-MB-468 cells as well as control cell line L929 to analyze the cytotoxic activity of the dichloromethane extract. In addition, Apoptosis induction of Urtica dioica on the MDA-MB-468 cells was assessed using TUNEL (terminal deoxy transferase (TdT)-mediated dUTP nick- end labeling) assay and DNA fragmentation analysis and real-time polymerase chain reaction (PCR)....
Source: Cellular and Molecular Biology - March 9, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The effect of celecoxib and its combination with imatinib on human HT-29 colorectal cancer cells: Involvement of COX-2, Caspase-3, VEGF and NF-κB genes expression.
In conclusion, the present in vitro study with colon cancer cell line demonstrated that CX and its combination with IM improved the anticancer activity of each component. Caspase-3 and COX-2 dependent molecular targets seem to be involved in mediating the anti-proliferative effects of IM and CX combination. Of course, the other molecular pathways are also likely to play the role and should be explored in future studies. PMID: 26950454 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - March 9, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Pravastatin and C reactive protein modulate protease- activated receptor-1 expression in vitro blood platelets.
In conclusion, PAR-1 served as a critical mechanism to relay platelet activation process induced by ADP. CRP and pravastatin reduce PAR-1 expression in platelet by ADP pathway. PMID: 26950455 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - March 9, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effects of c-Jun N-terminal kinase on Activin A/Smads signaling in PC12 cell suffered from oxygen-glucose deprivation.
Authors: Wang JQ, Xu ZH, Liang WZ, He JT, Cui Y, Liu HY, Xue LX, Shi W, Shao YK, Mang J, Xu ZX Abstract Activin A (Act A), a member of transforming growth factor-β (TGF-β) superfamily, is an early gene in response to cerebral ischemia. Growing evidences confirm the neuroprotective effect of Act A in ischemic injury through Act A/Smads signal activation. In this process, regulation networks are involved in modulating the outcomes of Smads signaling. Among these regulators, crosstalk between c-Jun N-terminal kinase (JNK) and Smads signaling has been found in the TGF-β induced epithelial-mesenchymal tra...
Source: Cellular and Molecular Biology - March 9, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Down-regulation of miR-144 after Mycobacterium tuberculosis infection promotes inflammatory factor secretion from macrophages through the Tpl2/ERK pathway.
Authors: Liu HY Abstract Pulmonary tuberculosis is one of the deadliest human diseases and mainly occurs when the immune system is impaired. MicroRNAs (miRNAs) have a critical role in regulating innate and adaptive immunity. Based on previous reports that Mycobacterium tuberculosis (M.tb) can modulate host cell miRNA expression, this study aimed to investigate expression changes in miR-144 and miR-144 regulate macrophage function via targeting of tumor progression locus 2 (Tpl2, also named MAP3K8) and extracellular signal-regulated kinase (ERK) signaling. I examined the miRNA expression profile of M.tb-infected mon...
Source: Cellular and Molecular Biology - March 9, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Characterization of Methicillin Resistant Staphylococcus aureus isolated from human and animal samples in Egypt.
Authors: Bendary MM, Solyman SM, Azab MM, Mahmoud NF, Hanora AM Abstract Staphylococcus aureus (S. aureus) has been one of the most problematic pathogens. Methicillin Resistant S. aureus (MRSA) has emerged as a major concern for both human and animal. Antibiotic resistance genes dissemination might be possible between human and animal bacteria. The aim of this study is to show phenotypic and genotypic diversity of human and animal MRSA isolates. Antibiogram typing and biofilm production were used as a primary phenotypic typing tool for the characterization of (40) animal and (38) human MRSA isolates. Genetic typing...
Source: Cellular and Molecular Biology - March 9, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Rapid amplification system for recombinant protein production in Chinese Hamster Ovary (CHO) Cells.
This study describes the development of a novel stable expression system for rapid amplification of genes in Chinese Hamster Ovary (CHO) cells. The expression system consists of a host CHO cell line and an expression vector (pUB-PyOri-D-C) which encodes for Polyomavirus (Py) Origin of Replication (PyOri) for amplification of integrated genes in the presence of Py Large T Antigen (PyLT) and Dihydrofolate Reductase (DHFR) selectable marker gene for selection in the presence of Methotrexate (MTX). Use of both PyOri/PyLT and DHFR can reduce the number of rounds of selection and amplification required for isolation of high prod...
Source: Cellular and Molecular Biology - March 9, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Gastric Cancer and Helicobacter pylori: Impact of hopQII Gene.
Authors: Kazemi E, Kahrizi D, Moradi MT, Sohrabi M, Yari K Abstract The Helicobacter pylori is a Gram-negative, microaerophilic bacterium found usually in the stomach and use a number of mechanisms to survive in the stomach lumen. The presence of these bacteria in the stomach can lead to gastritis and reduction in stomach acid production. Acute inflammation can directly damage to the peripheral cells that are responsible for the secretion of acid. The risk of developing gastric carcinoma is associated to heterogeneity of Helicobacter pylori virulence factors. The HopQII is one of the outer membrane proteins involve...
Source: Cellular and Molecular Biology - March 9, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Lentiviral mediated overexpression of Insulin like Growth Factor-1 in mouse myoblast.
Authors: Roudbari Z, Nassiri MR, Tahmoorespur M, Haddad-Mashadrizeh A Abstract Insulin-like growth factor gene (IGF-1) is one of the most important growth factors that plays a key role in the proliferation and differentiation of muscle cells. IGF-1 is also a radial stimulant in muscle hypertrophy in mammals. In our study, we constructed a lentiviral vector inducing an overexpression of IGF-1 in order to study the regulating mechanisms of this gene. The IGF-1 gene was cloned into the lentiviral shuttle plasmid pCDH-cGFP and the recombinant lentiviral vector was transducted into myoblast C2C12 cell line. The overexpr...
Source: Cellular and Molecular Biology - March 9, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

A study on local expression of NF-κB, CCL2 and their involvement in intratumoral macrophage infiltration in breast cancer.
A study on local expression of NF-κB, CCL2 and their involvement in intratumoral macrophage infiltration in breast cancer. Cell Mol Biol (Noisy-le-grand). 2016;62(2):116-25 Authors: Tewari BN, Singh Baghel K, Tripathi C, Dubey P, Bhatt ML, Kumar V, Mati Goel M, Singh Negi MP, Misra S Abstract NF-κB has been implicated in mechanisms promoting inflammation in tumor microenvironment leading to breast cancer metastasis. Owing to critical role of CCL2 during metastasis, particularly in its capacity to act as a chemoattractant for macrophages and their precursors i.e monocytes, we decided to expl...
Source: Cellular and Molecular Biology - March 9, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Protective effect of Honokiol against endometriosis in Rats via attenuating Survivin and Bcl-2: A mechanistic study.
Authors: Wang Y, Jiang LL, Wu JF, Liu Z Abstract Nearly 10-15% of women in the reproductive age were affected by endometriosis and currently facing the unmet need of effective therapeutic interventions for its management. Concerning this, the present study was intended to investigate the effect of Honokiol (HK) for the treatment of endometrial hyperplasia. The rat endometrial model was established and subsequently administered with a numerous dose of HK. The histopathology of tissues was also investigated. Results showed that, HK effectively inhibit the proliferation of rat edometeriotic tissues in a dose dependent...
Source: Cellular and Molecular Biology - February 2, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Association between Helicobacter pylori hopQI genotypes and human gastric cancer risk.
Authors: Kazemi E, Kahrizi D, Moradi MT, Sohrabi M, Amini S, Mousavi SA, Yari K Abstract The Helicobacter pylori use a number of mechanisms to survive in the stomach lumen and can lead to gastritis and reduction in stomach acid secretion. It has been found that the risk of developing gastric carcinoma is associated to heterogeneity of H. pylori virulence factors such as HopQ. The HopQ is one of the outer membrane proteins involved in bacterial adherence to gastric mucosa and has been suggested to also main role in the virulence of H. pylori. The purpose of the current study was to investigate the association betwee...
Source: Cellular and Molecular Biology - February 2, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

YiQi ChuTan Recipe Inhibits Epithelial Mesenchymal Transition of A549 Cells under Hypoxia.
This study aims to investigate mechanism of YiQi ChuTan Recipe (YCR) for inhibiting epithelial mesenchymal transition (EMT) of A549 cells under hypoxia. Flow cytometry was used to optimize YCR dosage by measuring A549 apoptosis, which were subjected to different treatments, including normal condition, hypoxia, hypoxia+YCR. Cell morphology and expression of EMT were measured with differential interference contrast microscopy, real-time PCR and western blot. Optimized condition of 4 mg/ml YCR and 2% O2 for 72 h was used to establish hypoxia. Under hypoxic condition, morphology of A549 cells changed from oblate fusi-form to e...
Source: Cellular and Molecular Biology - February 2, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

DNA Methylation and microRNA patterns are in association with the expression of BRCA1 in ovarian cancer.
Authors: Shariati-Kohbanani M, Zare-Bidaki M, Taghavi MM, Taghipour Z, Shabanizadeh A, Kennedy D, Dahim H, Salahshoor MR, Jalili C, Kazemi Arababadi M Abstract Ovarian cancer is the sixth most prevalent cancer in women and is considered the most lethal gynecological malignancy. It can be inherited as a familial disease but also has a strong spontaneous occurrence. Although the disease is associated with genome instability brought on by genetics and environmental factors there is evidence that mutations in the gene encoding for the breast cancer type 1 susceptibility protein (BRCA1) or its down-regulation are involv...
Source: Cellular and Molecular Biology - February 2, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Angiotensin II up-regulates monocarboxylate transporters expression in the rat adrenal gland.
Authors: Kirat D, Shousha S Abstract Angiotensin II (Ang II) is a major regulator of aldosterone secretion in the adrenal zona glomerulosa because it up-regulates the expression of a large number of genes involved in aldosterone biosynthesis. The transport of acetate across adrenocortical cells is a crucial step in the de novo synthesis of cholesterol, the steroid precursor of aldosterone. However, whether Ang II can affect this transport remains unknown. The current study aims to investigate the effect of in vivo infusion of Ang II on monocarboxylate transporters (MCT1, MCT2, and MCT4) gene expression in the rat a...
Source: Cellular and Molecular Biology - February 2, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Losartan sensitizes selectively prostate cancer cell to ionizing radiation.
In this study, we investigated the radiosensitizing effect of losartan on cellular toxicity induced by ionizing radiation on prostate cancer and non-malignant fibroblast cells. Human prostate cancer (DU-145) and human non-malignant fibroblast cells (HFFF2) were treated with losartan at different concentrations (0.5, 1, 10, 50 and 100 µM) and then these cells were exposed to ionizing radiation. The cell proliferation was determined using MTT assay. Our results showed that losartan exhibited antitumor effect on prostate cancer cells; it was reduced cell survival to 66% at concentration 1 µM. Losartan showed an ad...
Source: Cellular and Molecular Biology - February 2, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The E-selectin S149R polymorphisms in breast cancer in a northern Iran population.
This study was performed to evaluate the association of E-selectin S149R polymorphisms and the risk of breast cancer. A total of 100 patients with breast cancer and 120 healthy sample donors (controls) were recruited in this study. Genomic DNA was extracted from fresh blood samples and genotyping of the E-selectin genes was performed using PCR-restriction fragment length polymorphism (PCR-RFLP). The frequencies of the CC, AC and AA genotypes were 2%, 84%, and 14% in patients and 2%, 12%, and 86 % in controls, respectively, while the A and C allelic frequency was 56%, 44% in patients and 92%, 8% in controls, respectively. S...
Source: Cellular and Molecular Biology - February 2, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Cell-based evaluation of a novel Dictyophora indusiata polysaccharide against oxidative-induced erythrocyte hemolysis.
Authors: Liao W, Chen L, Yu B, Lei Z, Wu X, Yang J, Ren J Abstract The protective effectof a polysaccharide from Dictyophora indusiata(DP1)against oxidative hemolysis was comprehensively evaluated. The 2, 2-azobis (2-amidino-propane) dihydrochloride (AAPH)-induced erythrocyte hemolysis assay showed that DP1 exhibited excellent anti-hemolytic activity(87.4% hemolysis suppression ratio at 20 nmol/mL). Also, the formation of conjugated diene induced by cupric chloride (CuCl2) in plasma was significantly inhibited by DP1. Besides, DP1 could effectively inhibit AAPH-induced overproduction of reactive oxygen species (81....
Source: Cellular and Molecular Biology - February 2, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Signaling lansdscape of prostate cancer.
Authors: Lin X, Aslam A, Attar R, Yaylim I, Qureshi MZ, Hasnain S, Qadir MI, Farooqi AA Abstract Research over the decades has gradually and sequentially shown that both intratumor heterogeneity and multifocality make prostate cancer difficult to target. Different challenges associated with generation of risk-stratification tools that correlate genomic landscape with clinical outcomes severely influence clinical efficacy of therapeutic strategies. Androgen receptor mediated signaling has gained great appreciation and rewiring of AR induced signaling cascade in absence of androgen, structural variants of AR have pro...
Source: Cellular and Molecular Biology - February 2, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effects of genetic factors to stent thrombosis due to clopidogrel resistance after coronary stent placement.
In conclusion, it was suggested that there is a relation between CYP2C19*2 mutations and ST due to clopidogrel resistance, and CYP2C19*17 may have a protective role in this process. The use of novel and more potent drug or high clopidogrel maintenance dosing before stent implantation may be beneficial treatment options for antiplatelet therapy in CYP2C19*2 carriers. PMID: 26828987 [PubMed - as supplied by publisher] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - February 2, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

RT-PCR detection of exotoxin genes expression in multidrug resistant Pseudomonas aeruginosa.
Authors: Tartor YH, El-Naenaeey EY Abstract Pseudomonas aeruginosa (PA) is an opportunistic pathogen responsible for causing a wide variety of acute and chronic infections with significant levels of morbidity and mortality. These infections are very hard to eradicate because of the expression of numerous virulence factors and the intrinsic resistance against antibiotics. Herein, this study analyzed antimicrobial susceptibility of PA isolated from broiler chickens and cattle as well as expression of five significant exotoxin genes (exoU, exoS, toxA, lasB, and phzM) and ecfX as internal control. Genomic DNA was ampli...
Source: Cellular and Molecular Biology - February 2, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

All-trans retinoic acid inhibits HOXA7 expression in leukemia cell NB4.
In this study, we detected the expression of HOXA7 gene in human acute promyelocytic leukemia cell line NB4. The expression level of HOXA7 decreased in the presence of ATRA, which was able to inhibit the proliferation of NB4 cells. Furthermore, ATRA altered the morphology of NB4 cells. The study suggested that HOXA7 might be a new gene candidate that influences the maturation of acute myeloid leukemia, and provided the molecular basis for the treatment for acute promyelocyticleukemia. PMID: 26828989 [PubMed - as supplied by publisher] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - February 2, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Hypo/unmethylated promoter status of Cdk2 gene correlates with its over-expression in ovarian cancer in north Indian population.
Authors: Kanwal A, Kaur M, Singh A, Gupta S, Sachan M Abstract Cdk2 has been seen to play crucial roles in cell cycle regulation particularly in G1 to S phase transition and it is known to be involved in cancer progression and cancer cell proliferation. The aim of this study was to evaluate (i) the expression of cdk2 in malignancy of ovarian tumors and (ii) correlation between expression and DNA methylation. Clonal bisulfite sequencing and quantitative PCR analysis was performed for promoter methylation and expression analysis respectively in normal and ovarian tumor tissues. Increased methylation level was observe...
Source: Cellular and Molecular Biology - February 2, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Concentration analysis of hypoxi-inducible factor-1α; and vascular endothelial growth factor in patients with aortic aneurysm at different stages and its clinical significance.
In conclusion, serum HIF-1α and VEGF has clinical value of early diagnosis and assessment of disease severity. PMID: 26828991 [PubMed - as supplied by publisher] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - February 2, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Increase in oxidative stress and mitochondrial impairment in hypothalamus of streptozotocin treated diabetic rat: Antioxidative effect of Withania somnifera.
Authors: Parihar P, Shetty R, Ghafourifar P, Parihar MS Abstract Hypothalamus, the primary brain region for glucose sensing, is severely affected by oxidative stress in diabetes mellitus. Oxidative stress in this region of brain may cause severe impairment in neuronal metabolic functions. Mitochondria are prominent targets of oxidative stress and the combination of increased oxidative stress and mitochondrial dysfunctions may further decline hypothalamic neuronal functions. In the present study we examined the oxidative damage response, antioxidative responses and mitochondrial membrane permeability transition in h...
Source: Cellular and Molecular Biology - February 2, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Histamine H4 Receptor mediates interleukin-;8 and TNF-α release in human mast cells via multiple signaling pathways.
In conclusion, we found that H4R mediates the release of inflammatory cytokine TNF-α and chemokine IL-8 in human mast cells via PI3K, Ca2+-Calcineurin-NFAT and MAPKs signaling pathways. PMID: 26828993 [PubMed - as supplied by publisher] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - February 2, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The synergistic effect of ω3 and Vit D3 on glycemia and TNF-α in islet transplantation.
The synergistic effect of ω3 and Vit D3 on glycemia and TNF-α in islet transplantation. Cell Mol Biol (Noisy-le-grand). 2016;62(1):90-98 Authors: Gurol AO, Okten-Kursun A, Kasapoglu P, Suzergoz F, Kucuksezer UC, Cevik A, Tutuncu Y, Yentur SP, Gurol SD, Kucuk M, Yilmaz MT Abstract The current treatment of type 1 diabetes consists of insulin administration. Transplantation of islets of Langerhans is considered very favorable because the full effect of insulin treatment cannot be obtained in severe cases. Although agents such as omega-3 (ω3) and vitamin D3 (Vit D3) are known to contribut...
Source: Cellular and Molecular Biology - February 2, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Dicliptera Chinensis polysaccharides target TGF-β/Smad pathway and inhibit stellate cells activation in rats with dimethylnitrosamine-induced hepatic fibrosis.
In conclusion, this study revealed that DCP attenuates progression of liver fibrosis through suppressing TGF-β/Smad pathway. DCP is a potential botanical polysaccharide to management liver fibrosis. PMID: 26828995 [PubMed - as supplied by publisher] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - February 2, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

CXCL9 expression and polyomavirus BK infectivity in renal transplant patients with nephropathy.
Authors: Kariminik A, Yaghobi R, Dabiri S Abstract Polyomavirus BK is an important risk factor for nephropathy and renal lose after kidney transplantation. CXCL9 is a key immunoregulatory molecule which participates in stimulation and migration of immune cells to the infected sites. Thus, the main aim of this study was to evaluate the expression levels of CXCL9 mRNA and serum levels in the infected polyomavirus BK infected renal transplant patients with and without nephropathy compared with healthy controls. This cross sectional study was performed on three studied groups including: polyomavirus BK infected vs. non...
Source: Cellular and Molecular Biology - February 2, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Protective effect of ferulic acid against 2,2'-azobis(2-amidinopropane) dihydrochloride-induced oxidative stress in PC12 cells.
Authors: Shen Y, Zhang H, Wang L, Qian H, Qi Y, Miao X, Cheng L, Qi X Abstract Oxidative stress is closely related to the pathogenesis of neurodegenerative disorders such as Parkinson's disease and Alzheimer's disease. However, the underlying antioxidant mechanisms of ferulic acid (FA) aganist oxidantive stress are poorly understood. We evaluated the potential protective effects of FA against 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced damage in PC12 cells. Our results indicated that pretreatment with FA prior to AAPH exposure significantly increased PC12 cell survival, and also increased catalase ...
Source: Cellular and Molecular Biology - February 2, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

MicroRNA-92a Regulates Expression of Kruppel-like Factor2 in Rabbit Model of Intracranial Aneurysm.
In conclusion, our research demonstrated that miR-92a and KLF2 were negative correlation in intracranial aneurysm model, and miR-92a could directly target KLF2 in endothelial cells through complementary sequence of 3'UTR region. PMID: 26718427 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - January 13, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Association between Manganese Superoxide Dismutase (MnSOD Val-9Ala) genotypes with the risk of generalized aggressive periodontitis disease.
Authors: Kazemi E, Moradi MT, Yari K, Mousavi SA, Kahrizi D Abstract Generalized aggressive periodontitis (GAP) is a subtype of periodontal diseases that characterized by rapid destruction of periodontal supporting tissues. The MnSOD Val-9Ala mutation of manganese superoxide dismutase gene (MnSOD Val-9Ala) and its correlation with periodontal diseases has been studied in different populations. The purpose of this study was to investigate the possible association of MnSODVal-9Ala polymorphism with periodontitis disease in sample of GAP patients in Iran for the first time. Following a GAP examination, 50 GAP patients...
Source: Cellular and Molecular Biology - January 13, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Genetic variants in the tumor Necrosis Factor-Related Apoptosis-Inducing Ligand (TRAIL) do not contribute but Death Receptor (DR4) genes may contribute to susceptibility to head and neck cancer in Pakistani population.
Authors: Sarwar R, Mansoor Q, Farooqi AA, Shahzad S, Fayyaz S, Ismail M Abstract TRAIL mediated signaling in cancer cells has emerged as one amongst the most deeply studied molecular phenomenon. Recent breakthroughs have shown that overexpression of anti-apoptotic proteins, inactivation of pro-apoptotic proteins, transcriptional downregulation of TRAIL, DR4/DR5, degradation of DR/DR5 are some of the mechanisms which dramatically abrogate TRAIL induced apoptosis in cancer cells. Data obtained through genetic studies has highlighted highly polymorphic nature of DR4 and in accordance with this concept, we investigated...
Source: Cellular and Molecular Biology - January 13, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research