Effects of the lncRNA MALAT1 gene region rs664589 site mutation on acute myocardial infarction in Chinese Han
Cell Mol Biol (Noisy-le-grand). 2024 Jan 31;70(1):119-127. doi: 10.14715/cmb/2024.70.1.16.ABSTRACTWe aimed to study the association between the non-coding region of the lncRNA MALAT1 gene, the non-coding region rs664589 C>G variant, and the risk of acute myocardial infarction (AMI) in the Chinese Han population. 165 NSTEMI and 135 STEMI patients were enrolled in the study. An additional 150 healthy individuals were enrolled as the controls. All subjects were analyzed for the MALAT1 rs664589 locus genotype. The receiver operating curve (ROC) was used to determine the effect of MALAT1 rs664589 single nucleotide polymorphi...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Huamin Yu Sijia Tu Chaofeng Shen Xinghua Bai Jindong Sun Mingjuan Shi Cairong Li Hong Yuan Source Type: research
Effect of silencing SSB1 gene on the expression of NBS1 in irradiated rat submandibular gland cells
In this study, we irradiated rat submandibular gland (SMG) cells, which were either infected with a rAdE5-SSB1-1p2-shRNA recombinant adenovirus to silence SSB or a control virus, to explore the effect of IR on the expression NBS1 in the absence of SSB. Our results showed that the SSB1 mRNA transcripts and protein expression of SSB1 and NBS1 initially increased and decreased later with increased doses. The relative expression reached the highest levels when the SMG cells were irradiated with 2Gy of IR. Silencing the SSB1 gene suppressed the expression of both SSB1 and NBS1 regardless of irradiation. The expression of NBS1 d...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Linjing Gao Qiuli Lv Yude Huang Yiyang Fan Lixiang Zhao Yanfei Zhao Xian Wang Dongqin Mo Haoyu Lu Daiyou Wang Source Type: research
Identification and validation of a novel 9-gene signature of non-specific classification to predict prognosis in glioma patients
In conclusion, our study provides a robust 9-gene signature and a predictive nomogram for evaluating the prognosis of glioma patients, offering valuable insights into personalized treatment strategies.PMID:38372105 | DOI:10.14715/cmb/2024.70.1.18 (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Guangzhao Li Xiaowang Niu Xiang Li Bin Lin Fei Yang Zhong Wang Source Type: research
Clinical significance of miR-200a in systemic lupus erythematosus and renal damage in children
Cell Mol Biol (Noisy-le-grand). 2024 Jan 31;70(1):94-98. doi: 10.14715/cmb/2024.70.1.13.ABSTRACTThe purpose was to analyze the clinical significance of miR-200a in children with initially diagnosed SLE and renal damage. Children with initially diagnosed SLE (n=100) and healthy children (n=50) undergoing physical examinations during the same period were recruited. Disease activity of SLE children was determined based on SLEDAI (systemic lupus erythematosus disease activity index), and they were divided to SLEDAIā¤9 group and SLEDAI>9 group, respectively. Moreover, SLE children were divided to LN group and non-LN group b...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Haiyan Zhang Xiaoqing Zhou Qinghu Li Min Zheng Source Type: research
TMCO1 regulates cell proliferation, metastasis and EMT signaling through CALR, promoting ovarian cancer progression and cisplatin resistance
This study aimed to explore the involvement of Transmembrane and coiled-coil domains 1 (TMCO1) in ovarian cancer progression and its regulatory mechanisms in cisplatin resistance. Using the GEPIA database, we analyzed TMCO1 expression in ovarian cancer and normal tissues. In a cohort of 99 ovarian cancer patients, immunohistochemistry and immunofluorescence were employed to assess TMCO1 expression in tumor and adjacent tissues, correlating findings with clinical and pathological characteristics. TMCO1 overexpression and knockout cell models were constructed, and their impact on non-cisplatin-resistant (SK-OV-3) and cisplat...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Guangyu Sun Shan Gong Suwei Lan Ying He Yanhua Sun Zhengmao Zhang Source Type: research
Correlation analysis of vaginal flora and immune function Th1/Th2 imbalance in women with high-risk HPV infections in the female reproductive tract
Cell Mol Biol (Noisy-le-grand). 2024 Jan 31;70(1):128-133. doi: 10.14715/cmb/2024.70.1.17.ABSTRACTThe purpose of this study was to analyze the correlation between vaginal flora and immune function Type 1 helper T cells/Type 2 helper T cells imbalance in females having HPV infections at high risk within the female reproductive tract. We selected 150 female patients who visited our hospital for reproductive tract inflammation between March 2019 and March 2021. They were divided into high-risk HPV-positive and high-risk HPV-negative groups according to the results of the HPV tests. Vaginal flora composition, density, diversit...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Jingjing Zheng Rongwei Zhao Chunxia Deng Source Type: research
A Study of transcription factor MEF2A gene polymorphisms in patients with coronary artery disease
In conclusion, we identified a much higher incidence of MEF2A in people with coronary artery disease, and MEF2A may play a crucial role in cardiovascular pathophysiology. Patients and controls had considerably different genetics and frequency of alleles.PMID:38372109 | DOI:10.14715/cmb/2024.70.1.11 (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Kazhal Muhammad Sulaiman Source Type: research
The effects of dietary organic selenium on growth, body composition and hematological parameters of common Carp (Cyprinus carpio) reared in recirculating aquaculture system
Cell Mol Biol (Noisy-le-grand). 2024 Jan 31;70(1):87-93. doi: 10.14715/cmb/2024.70.1.12.ABSTRACTA feeding trial was conducted to examine the effects of different levels of dietary organic selenium (Se) on the growth, body composition, and hematological indices of common carp. Se element was supplemented to the basal diet at the rates of 0.0, 0.5, 1 and 2 mgkg-1. Fish were randomly stocked at a rate of 25 fish into 500L tanks in the Recirculating Aquaculture System (RAS) system for 10 weeks. Weight gain, final body weight, and specific growth rate were significantly higher (p<0.05) in fish fed 0.5 mgkg-1 followed by 1 an...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Ibrahim Abdulrahman Mustafa Samad Sofy Omar Source Type: research
Enhancing the efficiency and functionality of xylanase from Bacillus sp. RTS11: Optimization, purification, characterization, and prospects in kraft pulp bleaching
Cell Mol Biol (Noisy-le-grand). 2024 Jan 31;70(1):67-79. doi: 10.14715/cmb/2024.70.1.10.ABSTRACTBacillus sp. RTS11, a xylanolytic strain, was isolated from the Algerian desert rocks. Genetic analysis revealed a remarkable 98.69% similarity to Bacillus pumilus. We harnessed optimization techniques, including Plackett-Burman screening and Box-Behnken optimization design, to amplify xylanase production and activity. The outcome of these efforts was an optimized medium that yielded an impressive xylanase production titer of 448.89 U, a threefold increase compared to the non-optimized medium (146 U). The Purification of xylanas...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Sara Sahnoun Bilal Yahiaoui Aicha Benlounissi Abdenacer Mouffok Barbara Ernst Manawwer Alam Karim Houali Yacine Benguerba Source Type: research
Repair effect of neurotrophic factor III (NT-3) on rats with spinal injury model and its mechanism
Cell Mol Biol (Noisy-le-grand). 2024 Jan 31;70(1):56-61. doi: 10.14715/cmb/2024.70.1.8.ABSTRACTThe present study aimed to study the repair effect of neurotrophic factor III (NT-3) on spinal injury model rats and its mechanism. Wistar rats with spinal injury were established by accelerated compression stroke after the operation and divided into control group, model group, and NT-3 intervention group. The motor function of rats in each group was evaluated at different postoperative time points (3, 7, 14 d). HE staining was used to detect the changes in tissue structure and morphology of the injured spinal column in each grou...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Dexin Zou Huimin Wang Sibin Hao Feng Chen Source Type: research
The role of silymarin and MAPK pathway in the regulation of proliferation and invasion of non-small cell lung cancer cells
Cell Mol Biol (Noisy-le-grand). 2024 Jan 31;70(1):62-66. doi: 10.14715/cmb/2024.70.1.9.ABSTRACTWe aimed to explore the role of silymarin and mitogen-activated protein kinase (MAPK) pathway in the regulation of proliferation and invasion of non-small cell lung cancer cells. Non-small cell lung cancer cells were cultured and divided into groups and treated with drugs, and A blank control group was set up. The concentration of silymarin in the experimental group was 10 mg/L, 20 mg/L and 40 mg/L, respectively, which were recorded as groups A, B and C, and three repeated experiments were performed in each group. Absorbance (A v...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Jing Zhang Kaige Zhang Jing Li Jia Zhang Xiaohai Cui Source Type: research
High expression of VTA1 is an adverse prognostic factor in lung adenocarcinoma
Cell Mol Biol (Noisy-le-grand). 2024 Jan 31;70(1):46-55. doi: 10.14715/cmb/2024.70.1.7.ABSTRACTLung adenocarcinoma (LUAD) is a common pathological type of non-small cell lung cancer; identifying preferable biomarkers has become one of the current challenges. Given that VTA1 has been reported associated with tumor progression in various human solid cancers but rarely reported in LUAD, herein, RNA sequencing data from TCGA and GTEx were obtained for analysis of VTA1 expression and differentially expressed gene (DEG). Furthermore, functional enrichment analysis of VTA1-related DEGs was performed by GO/KEGG, GSEA, immune cell ...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Xingang Sun Ruixin Wu Xinjun Guan Changsheng Dong Dongze Qiu Guojie Xia Shouhan Feng Jinlong Duan Lei Zhang Source Type: research
MiR-146a alleviates acute lung injury via inhibiting Notch 1 signaling pathway targeting macrophage
Cell Mol Biol (Noisy-le-grand). 2024 Jan 31;70(1):34-39. doi: 10.14715/cmb/2024.70.1.5.ABSTRACTAcute lung injury (ALI) is associated with the leukocyte infiltration and inflammation. Previous studies have shown that miR-146a is a valid regulator of the macrophage polarization in vitro inflammatory model. However, it is unclear whether miR-146a plays a protective role in ALI via modulating macrophage inflammation. To explore the potential therapeutic effect mechanism of miR-146a on ALI. We analyzed the expression of miR-146a in acute injured lung tissues and differentiated macrophage. Lipopolysaccharide (LPS) and interleuki...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Qiumin Zhao Yan Wang Jing Zou Rong Kuang Shiyan Ji Source Type: research
Comparative study on the expression of Pax3, Rad51 and VEGF-C in esophageal gastric junction adenocarcinoma and distal gastric adenocarcinoma
Cell Mol Biol (Noisy-le-grand). 2024 Jan 31;70(1):40-45. doi: 10.14715/cmb/2024.70.1.6.ABSTRACTThe purpose of this study was to explore the differential expression of Pax3, Rad51 and VEGF-C in esophageal gastric junction adenocarcinoma and distal gastric adenocarcinoma and their relationship with cancer occurrence and development. 57 patients with gastric cancer were included and divided into esophageal gastric junction adenocarcinoma group (n=28) and distal gastric adenocarcinoma group (n=29). The positive expressions of Pax3, Rad51 and VEGF-C in the control group were lower than those in the esophageal gastric junction a...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Qiang Pan Xiangyang Liu Chen Xu Chengyuan Yu Source Type: research
MiR-22 inhibits myocardial fibrosis in rats with myocardial infarction by targeting PTEN/Akt/mTOR signaling pathway
This study aimed to evaluate the effects of miR-22 on myocardial fibrosis in rats with myocardial infarction (MI) and to further explore the possible underlying mechanism. A total of 80 rats were randomly divided into Sham group, miR-22 overexpression group, MI group or MI + miR-22 overexpression group. Reverse transcription-polymerase chain reaction (RT-PCR) results showed that compared with Sham group, miR-22 expression level in myocardial tissues of rats decreased significantly in MI group. Overexpression of miR-22 could remarkably relieve cardiac insufficiency in MI rats, increase EF% and FS%, and reduce collagen depos...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Bolin Li Chunna Liang Yuanjun Lv Yongjin Tan Weiyan Chen Source Type: research
