Ghrelin did not change coronary angiogenesis in diet-induced obese mice.
Authors: Khazaei M, Tahergorabi Z Abstract Ghrelin is a 28 amino acids peptide that initially was recognized as an endogenous ligand for growth hormone secretagogue receptor (GHSR). Recently, a number of studies demonstrated that ghrelin is a cardiovascular hormone with a series cardiovascular effect. The main objective of this study was to investigate the effect of systemic ghrelin administration on angiogenesis in the heart and its correlation with serum leptin levels in normal and diet-induced obese mice. 24 male C57BL/6 mice were randomly divided into four groups: normal diet (ND) or control, ND+ghrelin, high-f...
Source: Cellular and Molecular Biology - April 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The effects of melatonin on oxidative stress and prevention of primordial follicle loss via activation of mTOR pathway in the rat ovary.
This study indicated that melatonin may have a significant protective effect on primordial follicles and increase the expression of mTOR and downstream components in rat ovary. Melatonin treatment may have a beneficial effect on fertility. PMID: 28364790 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - April 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effect of nitrogen sources on some morphological characteristics of in vitro stevia rebaudiana Bertoni.
Authors: Akbari F, Arminian A, Kahrizi D, Fazeli A Abstract Stevia rebaudiana Bertoni belongs to Asteraceae family that leaves 200-300 times sweeter than sugar. Low seed fertility is one of the most important problems in Stevia production. So, Plant tissue culture is an efficient method for mass propagation of Stevia. In this research, we studied the effect of various concentrations of nitrogen on some morphological traits of stevia under in vitro conditions. We used axillary nodes as explants and they were cultured on Murashige and Skoog (MS) medium containing inorganic nitrogen sources i.e. NH4NO3(0, 825 and 1650...
Source: Cellular and Molecular Biology - April 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Identification of bacteria using volatile organic compounds.
Authors: Karami N, Karimi A, Aliahmadi A, Mirzajan F, Rezadoost H, Ghassempour A, Fallah F Abstract The rapid diagnosis of respiratory infections has always been an important goal for medical professionals, because rapid and accurate diagnosis leads to proper and timely treatment, and consequently, reduces the costs of incorrect and long-term treatments, and antibiotic resistance. The present study was conducted with the aim of detecting volatile organic compounds (VOCs) in three bacteria: Pseudomonas aeruginosa, Acinetobacter baumannii, Klebsiella pneumoniae. Headspace of the studied bacteria, after separately cul...
Source: Cellular and Molecular Biology - April 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Signaling networks in TMPRSS2-ERG positive prostate cancers: Do we need a Pied Piper or sharpshooter to deal with "at large" fused oncoprotein.
Signaling networks in TMPRSS2-ERG positive prostate cancers: Do we need a Pied Piper or sharpshooter to deal with "at large" fused oncoprotein. Cell Mol Biol (Noisy-le-grand). 2017 Feb 28;63(2):1-8 Authors: Lin X, Qureshi MZ, Romero MA, Yaylim I, Arif S, Ucak I, Fayyaz S, Farooqi AA, Mansoor Q, Ismail M Abstract Overwhelmingly increasing scientific evidence has provided near complete resolution of prostate cancer landscape and it is now more understandable that wide ranging factors underlies its development and progression. Increasingly it is being realized that genetic/epigenetic factors, In...
Source: Cellular and Molecular Biology - April 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Review of new insights into antimicrobial agents.
Authors: Dehghan Esmatabadi MJ, Bozorgmehr A, Hajjari SN, Sadat Sombolestani A, Malekshahi ZV, Sadeghizadeh M Abstract People have known the bacteria and have used various ways to deal with them, from a long time ago. Perhaps, natural antibiotics with have been the first step in fighting against pathogens. However, several factors, such as dealing with unfamiliar bacteria or emergence of drug-resistant species, have motivated us to discover new antibiotics or  even change previous types. In this regard, a variety of natural and synthetic antibiotics with different origins, mechanism of action, structures and f...
Source: Cellular and Molecular Biology - April 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The significance of DNA methylation profile in metastasis-related genes for the progression of colorectal cancer.
Authors: Tokarz P, Pawlowska E, Bialkowska-Warzecha J, Blasiak J Abstract DNA methylation, an epigenetic modification plays a role in the pathogenesis of colorectal cancer (CRC). CRC cases, both sporadic and familial, are often characterized by abnormal pattern of the cytosine methylation in CpG dinucleotides in regulatory regions of genes important for cancer transformation. Also genes mutated in CRC can have their epigenetic pattern altered and we suggest that changes in DNA methylation array can be important for CRC metastatic potential ‒ the main reason of CRC-associated mortality. These genes are: KRAS, gene...
Source: Cellular and Molecular Biology - April 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Comparison of two DNA extraction protocols from leave samples of Cotinus coggygria, Citrus sinensis and Genus juglans.
Authors: Fallah F, Minaei Chenar H, Amiri H, Omodipour S, Shirbande Ghods F, Kahrizi D, Sohrabi M, Ghorbani T, Kazemi E Abstract High quality DNA is essential for molecular research. Secondary metabolites can affect the quantity and quality DNA. In current research two DNA isolation methods including CTAB and Delaporta (protocols 1 & 2 respectively) were applied in three leave samples from Cotinus coggygria, Citrus sinensis and Genus juglans that their leaves are rich of secondary metabolites. We successfully isolated DNA from C. coggygria, C. sinensis and Genus Juglans using the two protocols described abo...
Source: Cellular and Molecular Biology - April 5, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Response of antioxidant system to formalin in the whole body of rainbow trout, Oncorhynchus mykiss.
Authors: İspir U, Kirici M, Yonar ME, Mişe Yonar S Abstract Formalin bath treatments are widely used to control of parasitic infections in aquaculture. However, effects of formaldehyde on the lipid peroxidation and antioxidant system were not still need elucidation. Fish were exposed to formalin at doses of 50, 100 and 200 ppm for 1 h. Fish were then left to depurate for 24 h in formalin-free water. At the end of the test, whole bodies were isolated and homogenized to measured malondialdehyde (MDA) and reduced glutathione  (GSH) levels and catalase (CAT) and glutathione peroxidase (GPx ) activities. Results ...
Source: Cellular and Molecular Biology - February 27, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

β-Catenin overexpression causes an increase in inflammatory cytokines and NF-κB activation in cardiomyocytes.
In this study, roles of β-catenin in inflammation in cardiomyocytes were investigated. 10 samples from hearts of patients with acute infarction and 10 from normal ones were collected in order to access roles of β-catenin in cardiomyocytes. H9c2 cardiomyoblasts and primary neonatal rat cardiomyocytes were transfected with porcine cytomegalovirus (pCMV)-β-catenin plasmid in order to overexpress β-catenin. Protein level of β-catenin protein was increased in human acute infarction tissues compared to ones from normal patients. The transcription factor had increased nuclear localization in cardiomyocyte...
Source: Cellular and Molecular Biology - February 27, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Induction of cellular proliferation in a human astrocytoma cell line by a Trypanosoma cruzi-derived antigen: a mechanism of pathogenesis?
Authors: Duran-Rehbein GA, Vargas-Zambrano JC, Cuéllar A, Puerta CJ, Gonzalez JM Abstract Trypanosoma cruzi can compromise the human central nervous system (CNS) during acute infection or reactivation in immune-suppressed hosts. Astrocytes have been identified as targets of T. cruzi's CNS infection in humans. Despite a high degree of parasitism and cellular lysis by T. cruzi in vitro the number of astrocytoma cells did not change when compared to uninfected cultures. This work evaluated cellular proliferation, changes in Major Histocompatibility Complex (MHC) expression as a reflection of antigen processing,...
Source: Cellular and Molecular Biology - February 27, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

CagA-positive and CagA-negative Helicobacter pylori strains differentially affect the expression of micro RNAs 21, 92a, 155 and 663 in human umbilical vein endothelial cells.
Authors: Kalani M, Hodjati H, GhamarTalepoor A, Samsami Dehaghani A, Doroudchi M Abstract Given that the basic mechanism of the effect of Helicobacter (H.) pylori in the induction of atherosclerosis remains unknown and regarding the regulatory role of micro RNAs (miRNAs) in endothelial cell (EC) functions, we aimed to investigate the effect of H. pylori on the expression of miRNAs involved in atherosclerosis (atheromiRs) and their correlation with apoptosis in human umbilical vein EC (HUVEC). HUVECs were treated with different cytotoxin associated gene A (CagA) positive and negative H. pylori derived products, then...
Source: Cellular and Molecular Biology - February 27, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effect of SOX2 on osteogenic differentiation of dental pulp stem cells.
Authors: Yuan J, Liu X, Chen Y, Zhao Y, Liu P, Zhao L, Han W Abstract To explore the effect of SOX2 on osteogenic differentiation of dental pulp stem cells (DPSCs) and to develop a new method for facilitating osteogenic differentiation of DPSCs. SOX2-overexpressing human DPSCs (DPSCs-SOX2) were established through retrovirus infection. Differentiation was induced with osteogenic differentiation medium and further evaluated by alkaline phosphatase (ALP) staining and qPCR. Following the differentiation procedure, the mechanism of SOX2 on osteogenic differentiation of DPSCs was analyzed using genome RNA microarray and...
Source: Cellular and Molecular Biology - February 27, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Beneficial effects of deep sea fish oil on diabetic mice neurological injury.
In conclusion, deep sea fish oil could be used as auxiliary health care products, which plays important role in preventing and treating implications of nerve lesion impairment induced by diabetes mellitus. PMID: 28234624 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - February 27, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Nitric oxide levels in response to the patients with different stage of diabetes.
This study supports the role of insulin resistance in increased NO levels in both diabetic and prediabetic patients. PMID: 28234625 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - February 27, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The role of insulin receptor substrate (IRS) proteins in oncogenic transformation.
Authors: Gorgisen G, Gulacar IM, Ozes ON Abstract Insulin Receptor Substrate (IRS) proteins are the main cytoplasmic adaptor molecules involved in transducing extracellular signals from receptors to downstream proteins. This protein family have pivotal roles on maintenance, distribution and regulation of signaling networks. Since IRS1/2 interact with and transmits signals from the receptors of insulin, Insulin Like Growth Factor 1 (IGF1), prolactin, growth hormone (GH), leptin, Vascular Endothelial Growth Factor (VEGF), TrkB, ALK and integrins this promoted scientist to think that IRS1 may have functions in cell pr...
Source: Cellular and Molecular Biology - February 27, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Application of stem cell for the regeneration of spiral ganglion neurons.
Authors: Mohammadian F, Eatemadi A, Daraee H Abstract Over 278 million of people worldwide suffers from hearing loss, and this disease has significant detrimental effects emotionally and economically on individuals and the society in totality. Treatment using cochlear implant dramatically improve the perception, and production of speech, as well as the patient quality of life, with the different sensorineural hearing loss (SNHL). Yet, there are some challenges faced by a cochlear implant. In this review, we propose the regeneration of spiral ganglion neurons which is an interface neuron using human amniotic fluid m...
Source: Cellular and Molecular Biology - February 27, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Inner ear drug delivery using liposomes.
Authors: Mohammadian F, Eatemadi A, Daraee H Abstract Dysfunction of inner ear can result in from several disease procedures and introduce a possibility for therapeutic intervention. The existence of a blood-cochlear obstacle and round window membrane restricts direct access into the inner ear and following inner ear drug, gene, protein and cell delivery. Several strategies have designed to increase drug delivery to the inner ear. One of main particles for inner ear drug delivery is liposome. Here we reviewed the application of liposomes in inner ear drug delivery. PMID: 28234628 [PubMed - in process] (Source: ...
Source: Cellular and Molecular Biology - February 27, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Association between circulating transforming growth factor- β1 level and polymorphisms in systemic lupus erythematosus and rheumatoid arthritis: A meta-analysis.
This study systemically reviewed evidence regarding the relationship between circulating blood transforming growth factor-β1 (TGF-β1) levels and systemic lupus erythematous (SLE) and rheumatoid arthritis (RA), and associations between TGF-β1 polymorphisms and susceptibility to SLE and RA.  We conducted a meta-analysis on the serum/plasma TGF-β1 levels in SLE and RA patients and healthy controls, and the associations between TGF-β1 +869 T/C, +915 C/G, and -509 T/C polymorphisms and SLE or RA risk. Twenty-eight studies were considered in this meta-analysis. Circulating TGF-β1 levels were si...
Source: Cellular and Molecular Biology - February 27, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The influence of dietary antioxidant on ovarian eggs and levels of vitamin E, C, A, astaxanthin, β-carotene and oxidative stres in tissues of Astacus leptodactylus (Eschscholtz) during reproduction.
In conclusion, the VE and AX in broodstock diets were the most effective antioxidants on the ovarian egg number and size of A. leptodactylus. PMID: 28145851 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - February 3, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Safranal prevents rotenone-induced oxidative stress and apoptosis in an in vitro model of Parkinson's disease through regulating Keap1/Nrf2 signaling pathway.
In this study, we aimed to investigate the potential effect of safranal on PD using an in vitro model of PD induced by rotenone. We found that safranal significantly inhibited rotenone-induced cell death in a dose-dependent manner. Moreover, safranal also markedly suppressed the reactive oxygen species (ROS) generation and cell apoptosis induced by rotenone. Further investigation showed that safranal inhibited the expression of kelch-like ECH-associated protein 1 (Keap1) and promoted the nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2) in rotenone-induced dopaminergic neurons. Meanwhile, the down...
Source: Cellular and Molecular Biology - February 3, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Over-expression of brain-derived neurotrophic factor in mesenchymal stem cells transfected with recombinant lentivirus BDNF gene.
This study was aimed at investigating the expression of brain-derived neurotrophic factor (BDNF) in mesenchymal stem cells (MSCs) modified with recombinant lentivirus bearing BDNF gene. Lentivirus vectors bearing BDNF gene were constructed. MSCs were isolated from rats and cultured. The lentiviral vectors containing BDNF gene were transfected into the MSCs, and BDNF gene and protein expressions were monitored with enhanced green fluorescent protein (EGFP). RT-PCR and Western blot were used to measure gene and protein expressions, respectibvely in MSCs, MSCs-EGFP and MSCs-EGFP-BDNF groups. Green fluorescence assay confirmed...
Source: Cellular and Molecular Biology - February 3, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effects of matrix types on formation and transformation of energy-accumulating substances in enhanced biological phosphorus removal (EBPR).
In this study, a laboratory investigation of activated sludge in A/O-SBR reactor was conducted to probe the effects of the matrix types on EBPR polyphosphate, intracellular polysaccharide, polyhydroxyalkanoates (PHA) formation and transformation. There is a decrease in anaerobic condition and an increase in aerobic condition for the intracellular glycogen of sodium propionate matrix and sodium acetate matrix. While the intracellular glycogen of glucose matrix shows a decreasing tendency in both anaerobic and aerobic reaction process. Sodium acetate matrix is beneficial to the formation of polyhydroxybutyrate (PHB), but the...
Source: Cellular and Molecular Biology - February 3, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Association between GPX1 Pro189Leu polymorphism and the occurrence of bladder cancer in Morocco.
Authors: Hadami K, Ameziane El Hassani R, Ameur A, Dakka N, Abbar M, Al Bouzidi A, Attaleb M, El Mzibri M Abstract Worldwide, Bladder cancer is the most frequent male malignancy. It is the third most common male malignancy in Morocco. The risk factors for developing bladder cancer are multiples including dietary conditions, environmental exposure and oxidative stress. GPX1 gene encoding for the human cellular antioxidant enzyme glutathione peroxidase1 is a key factor in the cell detoxification process. GPX1 Pro198Leu polymorphism is associated with a decrease of enzyme activity and may contribute to bladder cancer ...
Source: Cellular and Molecular Biology - February 3, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Class I Integron and β-lactamase encoding genes of multidrug resistance Salmonella isolated from pigeons and their environments.
In conclusion TEM-1 β-lactamase was characterized in 20% of Salmonella isolates from squabs while Class1 integron was characterized in 70% Salmonella isolates from squabs. PMID: 28145856 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - February 3, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Cytotoxicity study of benzo[a]pyrene on blood cells of Wistar rat in vitro.
Authors: Zhang J, Liu Y, Wei SZ Abstract The effect of benzo[a]pyrene (BaP) on Wistar rat lymphocyte activity, lymphocyle membrane integrity and erythrocytes hemolysis were examined using the MTT assay, the release of the lactate dehydrogenase (LD) and the hemoglobin release respectively in vitro. The results showed that lymphocyte activity had no remarkable response to BaP after a low dose of exposure. But the membrane change was found after higher dose of BaP exposure, and the significant release of LD was observed. The release of LD increased with exposure time prolonging and exposure dose increasing. The result...
Source: Cellular and Molecular Biology - February 3, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Analysis of serum Calcium, Magnesium, and Parathyroid Hormone in neonates delivered following preeclampsia treatment.
Authors: Vahabi S, Zaman M, Farzan B Abstract Due to the approximate clinical and biochemical manifestations of calcium and magnesium disturbances, with regard to the regulatory effects of parathyroid hormone (PTH), this present study is designed to analyze serum calcium (Ca), magnesium (Mg), and (PTH) at the time of birth, 24 hours afterwards in newborns after the mother has been treated with Mg-sulfate. We registered 86 term and preterm neonates (43 in each group) using simple census method delivered through vagina to preeclampsia pregnant women treated with Mg-sulfate immediately before ...
Source: Cellular and Molecular Biology - February 3, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Phytochemical properties of Iranian organic saffron stigma: antioxidant, anticancer and apoptotic approaches.
In this study, we compared chemical and antioxidant compounds of organic (OS) and inorganic (IOS) stigma of saffron and evaluated their anti-proliferative and apoptosis effects on cancer cells. Total antioxidant capacity of both saffron were characterized by FRAP, DPPH and Folin-Ciocalteu. HPLC and MTT methods were used to assay the amount of their secondary metabolites and anticancer effects, respectively. The expression of two apoptosis-related genes in treated cells evaluated by quantitative Real Time-PCR analysis. Our data indicated that OS has more secondary metabolites, antioxidant and cytotoxic properties compared t...
Source: Cellular and Molecular Biology - February 3, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Synergistic antibacterial effect of apigenin with β-lactam antibiotics and modulation of bacterial resistance by a possible membrane effect against methicillin resistant Staphylococcus aureus.
Synergistic antibacterial effect of apigenin with β-lactam antibiotics and modulation of bacterial resistance by a possible membrane effect against methicillin resistant Staphylococcus aureus. Cell Mol Biol (Noisy-le-grand). 2016 Dec 30;62(14):74-82 Authors: Akilandeswari K, Ruckmani K Abstract Methicillin-resistant Staphylococcus aureus (MRSA) infections are easily spread among infected patients, where resistance has dramatically increased resulted in serious health issues. Therefore, there is a need to develop alternative natural or combination drug therapies. Apigenin (AP) is a natural poly phe...
Source: Cellular and Molecular Biology - February 3, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Molecular talk of adipokines in dermatological diseases.
Authors: Aydin S Abstract Adipose tissue is not a passive tissue storing triglycerides, but one that releases hormones, enzymes, growth factors, and cytokines. Currently, known to release more than 600 adipokines, the adipose is an active tissue involved in energy homeostasis and serving paracrine, autocrine and endocrinal functions. Besides, some adipokines (irisin and ghrelin, for example) are synthesized in the sebaceous glands of the skin and play important roles in certain dermatological diseases. This review will provide a systemic overview of adipokines, focusing on their tissue and organ distribution, funct...
Source: Cellular and Molecular Biology - February 3, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

VEGF mediated signaling in oral cancer.
Authors: Lin X, Khalid S, Qureshi MZ, Attar R, Yaylim I, Ucak I, Yaqub A, Fayyaz S, Farooqi AA, Ismail M Abstract Increasingly it is being realized that oral cancer arises from genetic/epigenetic mutations, dysregulations of spatio-temporally controlled signal transduction cascades and loss of apoptosis. Epidemiological studies have provided a stronger association between tobacco use (chewed and smoked) and oral cancer. Nevertheless, alcohol has also gained attention as a significant risk factor, having a multiplicative synergistic cancer promoting effect with tobacco. Vascular Endothelial Growth Factor (VEGF) medi...
Source: Cellular and Molecular Biology - February 3, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The association between paraoxonase 1 gene polymorphisms and polycystic ovarian syndrome.
In conclusion, this meta-analysis suggested that PON1 polymorphisms were associated with PCOS risk. PMID: 28145863 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - February 3, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Histological and histochemical characterization of the digestive tract of European catfish (Silurus glanis Linnaeus, 1758).
In this study was to examined the morphology, histology and histochemically the digestive tract of European catfish, Silurus glanis. Two-four age S. glanis obtained from Çelik Lake (Gölbaşı-Adıyaman, Turkey). Six S. glanis, 59-71 cm length, 1.5-3.2 kg weight used for in the investigation. The body cavity was opened and samples of digestive tract (esophagus, stomach and intestine) were fixed in neutral buffered 10% formalin and embedded in paraffin. Dewaxed section (5-6 µm) were deparaffinized and stained with haematoxylin and eosin (H&E), Crossman triple, periodic acid-shiff (PAS), alcian blue ...
Source: Cellular and Molecular Biology - January 4, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Purification and characterization of polyphenol oxidase from corn tassel.
In this study, polyphenol oxidase (PPO) from corn tassel  was extracted and partially purified through  (NH4)2SO4 precipitation and gel filtration chromatography. Optimal temperatures for subsrates catechol and 4-methyl catechol were 40 °C and 30 °C, respectively. The optimal pH values were 8.0 for catechol and 6.0 for 4-methyl catechol. Catechol was the most suitible substrate (Km: 3.48 mM, Vmax: 1.0 Abs./ min.). The moleculer mass of PPO was determined as 158 kDa. In this work, sodium azide, ethylenediaminetetraacetic acid (EDTA) and sodium dodecyl sulfate (SDS) were found to inhibit the enzyme act...
Source: Cellular and Molecular Biology - January 4, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The regulatory effects of autophagy to the CNE2 cells radio-sensitization.
Authors: Li BX, Cao XF, Weng CY, Fang XS, Mao HB, Guan MM, Liu GL Abstract To investigate the effects of autophagy activator and autophagy inhibitor on the CNE2 radiation sensitivity of nasopharyngeal carcinoma cells. RNA interference technology was used to silence the atg5 gene and autophagy inhibition cell model was constructed. Rapamycin and chloroquine were treated respectively on cells with X-ray 5Gy irradiation. Cells' growth status were observed for 8 days and control group was set. The cell viability was detected by MTT assay and colony formation assay, and the cell cycle was analyzed by flow cytometry. Com...
Source: Cellular and Molecular Biology - January 4, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The effects of mebudipine on myocardial arrhythmia induced by ischemia-reperfusion injury in isolated rat heart.
Authors: Ghiasi R, Mohammadi M, Majidinia M, Yousefi B, Ghiasi A, Badalzadeh R Abstract Reperfusion of the heart after an ischemic insult may lead to potentially lethal arrhythmias and cardimyocyte cell death via apoptosis and necrosis. In addition, previous studies showed that calcium channel blockers may have a protective role in the myocardium against arrhythmia and irreversible tissue injury. Therefore, this study was aimed to investigate the effects of mebudipine on myocardial arrhythmias and tissue injury induced by ischemia/reperfusion injury in isolated rat hearts. Male Wistar rats (250‑300g) were randoml...
Source: Cellular and Molecular Biology - January 4, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) gene mutations in North Egyptian population: implications for the genetic diagnosis in Egypt.
Authors: El-Seedy A, Pasquet MC, Shafiek H, Morsi T, Kitzis A, Ladevèze V Abstract Cystic fibrosis (CF) occurrence in Arab populations is not common and still remains underidentified. Furthermore, the lack of disease awareness and diagnosis facilities have mislead the identification of cystic fibrosis for decades. The knowledge about cystic fibrosis (CF) in Egypt is very limited, and a few reports have drawn attention to the existence of CF or CFTR-related disorders (CFTR-RDs) in the Egyptian population. Therefore a comprehensive genetic analysis of the CFTR gene was realized in patients of North Egypt. DNA ...
Source: Cellular and Molecular Biology - January 4, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Validation of housekeeping genes for the normalization of RT-qPCR expression studies in oral squamous cell carcinoma cell line treated by 5 kinds of chemotherapy drugs.
This study provides useful information to normalize gene expression accurately for the investigation of target gene profiling in cell lines of OSCC. Further clarification of tumor molecular expression markers with our recommended housekeeping genes may improve the accuracy of diagnosis and estimation of prognostic factors as well as provide novel personalized treatments for OSCC patients. PMID: 28040059 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - January 4, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Oxidative stress and some biochemical parameters during starvation and refeeding in Astacus leptodactylus (Esch., 1823).
This study was conducted to determine the effect of starvation (78 days) and refeeding (33 days) on oxidative stress (malondialdehyde (MDA)),  antioxidants (superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), glutathione peroxidase (GSH-Px)), arginase (AR) and nitric oxide (NO) in the hepatopancreas, muscle and gills tissues of freshwater crayfish (Astacus leptodactylus). Crayfish were maintained at three experimental groups: control (fed), starved (not fed) crayfish for 78 days and refeeding crayfish for 33 days after 78 days starvation. The biochemical analysis in the tissues were measured at 3, ...
Source: Cellular and Molecular Biology - January 4, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Preservation effect of meat product by natural antioxidant tea polyphenol.
Authors: Wang WD, Sun YE Abstract 1% of tea polyphenol, chitosan solution and potassium sorbate were used as film-forming materials to coat chilled mutton. Total coliforms, TVB-N value and pH value were determined and used as the mutton fresh-keeping indexes. The results showed that after 12th day at the end of the storage, mutton coated with tea polyphenol had best effects comparing chitosan solution and potassium sorbate. pH value of mutton coated by tea polyphenol was 6.0, TVB-N and the total coliforms were both significantly lower than the meat coated by chitosan solution and potassium sorbate. Also, mutton coa...
Source: Cellular and Molecular Biology - January 4, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Influences of dietary omega-3 fatty acids on haematologic and immunologic changes in rainbow trout milked regularly.
In conclusion, the omega-3 fatty acid supplementation at 2% level to the diet can effectively support the haematological and immunological values in the rainbow trout under regular milking conditions, and ultimately protect the health. PMID: 28040062 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - January 4, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Pivotal role of glutathione depletion in eNOS uncoupling of LPS-Treated HUVECs.
Authors: Chen L, Liu E, Cao D, Li S, Xiao C, Xiong M, Kou Q Abstract The present study investigated the relationship between uncoupling of endothelial nitric oxide synthase (eNOS) and vascular endothelial cell (VEC) oxidative stress (OS) during sepsis and the role of eNOS glutathionylation in eNOS uncoupling of septic VECs. Human umbilical vein endothelial cells (HUVECs) cultured in vitro (EA.hy269 cell line) were incubated with Dulbecco's modified Eagle's medium (DMEM) (normal control group), lipopolysaccharide (LPS) (sepsis group), 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) (glutathionylation group), and LPS+ di...
Source: Cellular and Molecular Biology - January 4, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

KLF5 is involved in regulation of IFITM1, 2, and 3 genes during H5N1 virus infection in A549 cells.
In conclusion, we revealed KLF5 is involved in transcription of IFITM1, 2, and 3 thus making it a potential therapeutic activator in the future to treat viral infections through promoting the innate immune response. PMID: 28040064 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - January 4, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effects of PAX9 and MSX1 gene variants to hypodontia, tooth size and the type of congenitally missing teeth.
In conclusion, especially new mutations which may cause hypodontia, effect tooth size and type of congenitally missing teeth, should be investigated with other researchers for clarifying the mechanism. PMID: 28040065 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - January 4, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effects of ambroxol hydrochloride on concentrations of paclitaxel and carboplatin in lung cancer patients at different administration times.
In this study, 470 cases of non-small cell lung cancer (NSCLC) were divided into different groups with ambroxol hydrochloride administered at different time points prior to chemotherapy, while another 171 cases received no ambroxol hydrochloride prior to chemotherapy. The results showed the concentrations of paclitaxel and carboplatin in sputum of patients treated with ambroxol hydrochloride were significantly higher than those of the control group, suggesting that ambroxol hydrochloride significantly increased the local concentrations of chemotherapeutic agents in lung tissues of NSCLC. Furthermore, the intravenous admini...
Source: Cellular and Molecular Biology - January 4, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Type 2 diabetes and mental disorders; a plausible link with inflammation.
Authors: Hajebrahimi B, Kiamanesh A, Asgharnejad Farid AA, Asadikaram G Abstract Mental disorders including depression and anxiety are the prevalent human diseases which are associated with several metabolic and non-metabolic diseases. Recent investigations demonstrated that the mental disorders can be considered as the potential inducers/stimulators of chronic inflammation. Based on the fact that chronic inflammation is a main cause of human diseases, hence, a hypothesis has been raised to explore the interaction between mental disorders and inflammation related metabolic diseases. Type 2 diabetes (T2D), as a comp...
Source: Cellular and Molecular Biology - January 4, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Association between alpha-adducin gene rs4963 polymorphism and hypertension risk in Asian population: a meta-analysis.
In conclusion, this meta-analysis suggested that ADD1 rs4963 polymorphism might be associated with increased hypertension risk. PMID: 28040068 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - January 4, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The effect of the ginger on the apoptosis of hippochampal cells according to the expression of BAX and Cyclin D1 genes and histological characteristics of brain in streptozotocin male diabetic rats.
Authors: Molahosseini A, Taghavi MM, Taghipour Z, Shabanizadeh A, Fatehi F, Kazemi Arababadi M, Eftekhar Vaghefe SH Abstract Diabetes is the most common endocrine disorder in humans with multiple complications including nervous system damages. The aim of the present study was to determine the effect of ginger extract on apoptosis of the neurons of hippocampus, via evaluation of BAX and Cyclin D1 and also histological analysis, in male diabetic rats. In this experimental study, 60 Wistar rats (220 ± 30gr) were conducted in 5 groups as follow: diabetic group treated with saline (group 1), normal group treated ...
Source: Cellular and Molecular Biology - December 1, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Differences of cell surface marker expression between bone marrow- and kidney-derived murine mesenchymal stromal cells and fibroblasts.
This study demonstrates that murine MSC isolated from different organs express certain specific markers, which enable their discrimination. PMID: 27894394 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - December 1, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Relationship between hepatitis B virus reverse transcriptase 181 mutation and S gene mutation in hepatitis B virus chronically infected patients.
In conclusion, mutation sites of pre-existing and adefovir dipivoxil induced resistance were different. HBV genotype C is prone to occur resistance mutation than genotype B. rtA181T mutation was accompanied with not only sW172 * mutation, but also sW172L mutation, rtA181V mutation was accompanied with sL173F mutation or Pre-S2 initiation codon to termination mutation. PMID: 27894395 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - December 1, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research