Association between platelet to lymphocyte ratio (PLR) and overall survival (OS) of hepatocellular carcinoma (HCC): A meta-analysis.
In conclusion, this meta-analysis suggested that PLR was significantly associated with the OS of HCC. PMID: 28886327 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - September 9, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Angiotensin-converting enzyme (ACE) I/D polymorphism is a risk factor of allergic rhinitis.
In conclusion, this meta-analysis indicated that ACE I/D polymorphism was significantly associated with allergic rhinitis risk. PMID: 28886328 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - September 9, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

A case of IgG4-related retroperitoneal fibrosis with multiple involvement.
We report a 79-year-old man with multiple organs involvement of IgG4-RRPF, who developed right lower extremity edema, hemoptysis and fever. The abdomen computed tomography (CT) scan image showed lymph nodes enlargement. The positron emission tomography/CT scan image showed pancreatic malignancy with multiple nodal lymph node metastasis, lung fibroblast proliferation, and right lung apex bullae. The chest CT scan image showed pulmonary multiple lymph nodes with calcification in the mediastinum. Posterior peritoneum magnetic resonance imaging showed the body and tail of the pancreas parenchymatous mass. The serum IgG4 concen...
Source: Cellular and Molecular Biology - September 9, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Cornelia de lange syndrome with thyroid agenesis of an indonesian patient.
We present an 11 months old female Indonesian patient with classic CdLs with congenital hypothyroid. Genetics studies were performed in intron 1, exon 2, exon 10 and exon 22 of NIPBL gene. Thyroid studies (T3, T4, TSH and thyroid scan) were performed. Low level of T3 and T4, and high level of TSH were observed. Thyroid agenesis was found in thyroid scan examination. We detected thyroid agenesis which has been never reported in CdLs patients. We could not find any mutation in intron 1, exon 2, exon 10 and exon 22 of NIPBL gene. Further genetics examinations were necessary whether there is mutation in other locus. PMID: ...
Source: Cellular and Molecular Biology - September 9, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Oncolytic E1B 55KDa-deleted adenovirus replication is independent of p53 levels in cancer cells.
In this study, we investigated the relationship between the efficiency of Ad5dlE1B 55kDa replication and p53 levels in cancer cells. Five transient p53 expression vectors were engineered to expresses different p53 levels in transfected cells. Then, the effect of the variable p53 levels and cellular backgrounds on the replication efficiency of oncolytic Ad5dlE1B 55kDa was evaluated in H1299 and HeLa cell lines. We found that the replication efficiency of these oncolytic viruses is dependent on the status, but not the expression levels, of p53. Ad5dlE1B 55kDa was shown to have selective replication activity in H1299 cells (p...
Source: Cellular and Molecular Biology - August 26, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Hypoxia stimulates neural stem cell proliferation by increasing HIF ‑1α expression and activating Wnt/β-catenin signaling.
Hypoxia stimulates neural stem cell proliferation by increasing HIF‑1α expression and activating Wnt/β-catenin signaling. Cell Mol Biol (Noisy-le-grand). 2017 Aug 15;63(7):12-19 Authors: Qi C, Zhang J, Chen X, Wan J, Wang J, Zhang P, Liu Y Abstract Evidence indicates that after brain injury, neurogenesis is enhanced in regions such as hippocampus, striatum, and cortex. To study the role of hypoxia-inducible factor-1 (HIF‑1α) and Wnt signaling in cerebral ischemia/hypoxia-induced proliferation of neural stem cells (NSCs), we investigated the proliferation of NSCs, expression of HIF...
Source: Cellular and Molecular Biology - August 26, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effects of curcumin plus Soy oligosaccharides on intestinal flora of rats with ulcerative colitis.
Authors: Huang G, Ye L, Du G, Huang Y, Wu Y, Ge S, Yang Z, Zhu G Abstract To explore the therapeutic effect of curcumin (Cur) and soybean oligosaccharides (SBOS) on ulcerative colitis (UC) through testing the intestinal flora and ulcerative colitis (UC). 80 male SD rats were selected divided into four groups with 20 rats in each group: normal group, sulfasalazine (SASP) group, model group and group of curcumin plus soy oligosaccharide. All animals were treated for 4 weeks. In the fifth week rats were decapitated. Macroscopic damage scores of colonic mucosa were calculated. A 4mL blood sample was taken to detect the...
Source: Cellular and Molecular Biology - August 26, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Comparative determination of ventilatory efficiency from constant load and incremental exercise testing.
Authors: Algul S, Ugur FA, Ayar A, Ozcelik O Abstract The analysis of the relationships between minute ventilation (VE) to CO2 output (VCO2), referred to as ventilatory efficiency, in response to incremental exercise testing, is considered a useful index for assessing the presence and severity of cardiopulmonary and metabolic diseases. The effects of constant load exercise testing performed at work intensity associated with anaerobic threshold (AT) and respiratory compensation points (RCP), on the accurate measurements of ventilatory efficiency are not well known. The aim of this present study was to investigate th...
Source: Cellular and Molecular Biology - August 26, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effect of Cdc42 on myocardial ischemia-reperfusion of rats.
Authors: Xu X, Kong L, Song X, Hao Z, Yuan F Abstract To investigate the effects and their possible mechanisms of cell division cycle 42 (Cdc42) to neonatal rat myocardial cells subjected to the ischemia-repefusion. Neonatal rat cardiomyocytes were cultured and then subjected to the ischemia-reperfusion. Experimental groups 1. Control group; 2. Ischemia-repefusion group (I/R group); 3. Oligofectamine group (Oli group); 4. Oligofectamine and antisense oligodeoxynucleotide (AS-ODN) group (As group); 5. Oligofectamine and missense oligodeoxynucleotide (MS-ODN) group (Ms group); 6. SP600125 and Oligofectamine and AS-OD...
Source: Cellular and Molecular Biology - August 26, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Mass spectrometric identification of diagnostic markers for chronic prostatitis in seminal plasma by analysis of seminal plasma protein clinical samples.
In conclusion, we found age-related changes in seminal plasma protein expression. PAEP expression in seminal plasma should be investigated further to evaluate its potential as a diagnostic marker for chronic prostatitis. PMID: 28838337 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - August 26, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Ghrelin and NUCB2/Nesfatin-1 expression in unilateral testicular torsion-induced rats with and without N-acetylcysteine.
Authors: Sarac M, Bakal U, Tartar T, Kuloglu T, Yardim M, Artas G, Aydin S, Kazez A Abstract Testicular torsion (TT) is a common urological problem in the field of pediatric surgery. The degree and duration of torsion determines the degree of testicular damage; however, its effects on the expression of octanoylated ghrelin and nucleobindin 2 (NUCB2) /nesfatin-1 synthetized from testicular tissue remain unclear. We explored the effects of experimentally induced unilateral TT on serum and contralateral testicular tissue ghrelin and NUCB2/nesfatin-1 levels, and determined whether N-acetyl cysteine (NAS) treatment had ...
Source: Cellular and Molecular Biology - August 26, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Histologic analyses of different concentrations of TritonX-100 and Sodium dodecyl sulfate detergent in lung decellularization.
In this study, we aimed to investigate a comparison of different concentrations of Triton X-100 and Sodium dodecyl sulfate detergents in lung decellularization in order to evaluate the effects of different concentrations and times of mentioned detergents on three dimensional and ECM proteins lung. Two detergents (Triton-X100 and Sodium dodecyl sulfat) were used with different concentrations for decellularizing rat lungs for maintaining of three-dimensional lung architecture and ECM protein compositions which have significant roles in differentiation and migration of stem cells. Results showed that SDS 0.05%, 0.1% and Trito...
Source: Cellular and Molecular Biology - August 26, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Isolation, identification and characterization of Morganella morganii from Naja naja atra in Beijing, China.
We report the isolate of M. morganii from Naja naja atra following infections of heart, lung and liver. Seven strains were confirmed using 16S rDNA amplified and sequences. Antimicrobial susceptibility testing of M. morganii isolates demonstrated ubiquitous resistance to ampicillin, amoxicillin/clavulanic acid, cefazolin, cephalothin, sulfamethoxazole/trimethoprim, sulfamethoxazole et al. However, M. morganii ubiquitous susceptible to piperacillin, ampicillin/sulbactam, piperacillin/tazobactam, cefixime et al. Further investigate display gyr B and Sul2 genes presence in all M. morganii isolates. AAC(3)-II was found in E2, ...
Source: Cellular and Molecular Biology - August 26, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Chicken egg-white extracts promote OCT4 and NANOG expression and telomeres growth in 293T cells.
Authors: Ruan GP, Yao X, Shu J, Liu JF, Pang RQ, Pan XH Abstract It will have broad applications in cell biology if one of egg cell extracts has the roles to promote cell proliferation and reprogramming. It will provide a new method for easier reprogramming somatic cells and promote cell proliferation. We found chicken egg-white extracts have roles to promote cell proliferation and reprogramming. The different ingredients were then assessed for cell proliferation activity and somatic cell reprogramming. Chicken egg-white extract ingredients that were less than 3 kDa (LT3K) promoted cell proliferation. Those ingredi...
Source: Cellular and Molecular Biology - August 26, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The association between PAI-1 -675 4G/5G polymorphism and type 2 diabetes mellitus.
In conclusion, this case-control study suggested that PAI-1 -675 4G/5G polymorphism was not associated with T2DM risk in this population. PMID: 28838342 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - August 26, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

In vitro evaluation of cytotoxic, anti-proliferative, anti-oxidant, apoptotic, and anti-microbial activities of Cladonia pocillum.
Authors: Ersoz M, Coskun ZM, Acikgoz B, Karalti I, Cobanoglu G, Cesal C Abstract The aim of this study was to investigate the anti-proliferative, apoptotic, cytotoxic, and anti-oxidant effects of extracts from the lichen Cladonia pocillumon human breast cancer cells (MCF-7), and to characterize the anti-microbial features.  MCF-7 cells were treated with methanolic C. pocillum extract for 24h. The cytotoxicity of the extract was tested with MTT. Moreover, its anti-proliferative effects were examined with immunocytochemical method. Apoptosis and biochemical parameters were detected in MCF-7. The methanol and chl...
Source: Cellular and Molecular Biology - August 26, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Inflammatory and immune responses to a 3-day period of downhill running in active females.
Authors: Jafariyan S, Monazzami A, Nikosefat Z, Nobahar M, Yari K Abstract Exercise-induced muscle damage (EIMD) is accompanied by inflammatory and immune responses. However, due to the repeated bout effect, there will probably be less EIMD. Hence, the purpose was to investigate inflammatory and immune responses over a three-day period of downhill running in active females. Eleven moderately trained healthy females performed three 60-minute bouts of downhill running in -13.5% grade, separated by 24 hours, at a speed eliciting 70-80% of their VO2peak on level grade. Delayed onset muscle soreness (DOMS), range of mot...
Source: Cellular and Molecular Biology - August 26, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Prediction of G gene epitopes of viral hemorrhagic septicemia virus and eukaryotic expression of major antigen determinant sequence.
In conclusion, the main antigen domain of VHSV G was successfully expressed in the Bac-to-Bac baculovirus system. PMID: 28838345 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - August 26, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Molecular effects of leptin on peroxisome proliferator activated receptor gamma (PPAR- γ) mRNA expression in rat's adipose and liver tissue.
Molecular effects of leptin on peroxisome proliferator activated receptor gamma (PPAR-γ) mRNA expression in rat's adipose and liver tissue. Cell Mol Biol (Noisy-le-grand). 2017 Aug 15;63(7):89-93 Authors: Abbasi A, Moghadam AA, Kahrarian Z, Abbsavaran R, Yari K, Alizadeh E Abstract Leptin is a 16-kDa peptide hormone secreted by adipose tissue that participates in the regulation of energy homeostasis. The aim of this study was to determine the effect of leptin injection on mRNA expression of peroxisome proliferator-activated receptor gamma (PPAR-γ) and comparison of PPAR-γ mRNA express...
Source: Cellular and Molecular Biology - August 26, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Synaptosomal and mitochondrial oxidative damage followed by behavioral impairments in streptozotocin induced diabetes mellitus: restoration by Malvastrum tricuspidatum.
Authors: Solanki I, Parihar P, Shetty R, Parihar MS Abstract Synaptosomal and mitochondrial impairments in the brain of diabetic individual manifest metabolic risk factors that most likely affect the brain functions. Diabetes is associated with the structural and functional alterations of the brain and neuronal loss leading to cognitive and other behavioral impairments, thus reducing the quality of life. The biochemical mechanisms underlying the diabetes-associated behavioral decline are poorly understood. In the present study, we examined the effects of hyperglycemia on the oxidative stress and behaviora...
Source: Cellular and Molecular Biology - August 26, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effect of salinity on gene expression, morphological and biochemical characteristics of stevia rebaudiana Bertoni under in vitro conditions.
Authors: Fallah F, Nokhasi F, Ghaheri M, Kahrizi D, Beheshti Ale Agha A, Ghorbani T, Kazemi E, Ansarypour Z Abstract Stevia rebaudiana Bertoni is a famous medicinal plant for its low calorific value compounds which are named steviol glycosides (SGs) and they are 150-300 times sweeter than sugar. Among various SGs, stevioside and rebaudioside A considered to be the main sweetening compounds.  Soil salinity is one of the most essential stress in the world. Salinity affects the survival and yield of crops. In current study the effects of salinity and osmotic stress caused by different concentration of NaCl (0, 20...
Source: Cellular and Molecular Biology - August 26, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effect of KH2PO4 on gene expression, morphological and biochemical characteristics of stevia rebaudiana Bertoni under in vitro conditions.
Authors: Kahrizi D, Ghari SM, Ghaheri M, Fallah F, Ghorbani T, Beheshti Ale Agha A, Kazemi E, Ansarypour Z Abstract Stevia rebaudiana is one of the most important biologically sourced and low-calorie sweeteners Bertoni that has a lot of steviol glycosides. Tissue culture is the best for propagation of stevia and micro nutrients can affect both morphological traits and steviol glycosides production. Therefore, the effect of different concentrations of KH2PO4on stevia growth factors and gene expression had been studied by tissue culture methods, RT-PCR and HPLC. According the results, bud numbers had increased signif...
Source: Cellular and Molecular Biology - August 26, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Molecular identification of drug resistant mutations to tetracycline in Mycoplasma spp. isolated from patients with multiple sclerosis.
Authors: Naghib M, Kheirkhah B, Mohebbi R, Sadeg L Abstract Bacterial infections play a significant role in causing or intensifying the attacks in MS and there are reports based on the interference of Mycoplasma with a global distribution. Mycoplasma causes autoimmune attacks by imitating the host cell membrane, which is a way of resistance to antibiotics. The purpose of this study was to evaluate the molecular identification of mutations causing resistance to tetracycline in Mycoplasma isolated from MS patients. A total number of 32 cerebrospinal fluid samples and 48 urinal fluid samples were collected from MS pat...
Source: Cellular and Molecular Biology - August 26, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Determination of some heavy metals in Mastacembelus mastacembelus (Banks & Solander, 1794) in terms of public health.
In conclusion the amounts of heavy metals in the flesh of spiny eels were found lower than that recommended by EPA, WHO, FAO and TFC. PMID: 28719337 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - July 20, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

miR-675 promotes disease progression of non-small cell lung cancer via activating NF- κB signaling pathway.
miR-675 promotes disease progression of non-small cell lung cancer via activating NF-κB signaling pathway. Cell Mol Biol (Noisy-le-grand). 2017 May 20;63(5):7-10 Authors: Feng Y, Yang C, Hu D, Wang X, Liu X Abstract Non-small cell lung cancer (NSCLC) is a major type of lung cancer significantly affected human life. Current studies have revealed potential role of miR-675 in the etiology of lung cancer. Therefore, in this study, we aimed to investigate the role of miR-675 in NSCLC cell line. We recruited 92 cases of NSCLC patients from July 2009 to February 2011 in the oncology department. Tumor an...
Source: Cellular and Molecular Biology - July 20, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Modulation of oxidative and glycolytic skeletal muscle fibers Na+/H+ exchanger1 (NHE1) and Na+/HCO3- co-transporter1 (NBC1) genes and proteins expression in type 2 diabetic rat (Streptozotocin + high fat diet) following long term endurance training.
In conclusion, endurance training may improve the capacity of pHi regulation in muscles by lactate-independent pathway. PMID: 28719339 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - July 20, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Sequence comparison of phoR, gyrB, groEL, and cheA genes as phylogenetic markers for distinguishing Bacillus amyloliquefaciens and B. subtilis and for identifying Bacillus strain B29.
Authors: Yu C, Jin J, Meng LQ, Xia HH, Yuan HF, Wang J, Yu DS, Zhao XY, Sha CQ Abstract Given the close genetic relationship between Bacillus amyloliquefaciens and B. subtilis, distinguishing the two solely based on their physiological and biochemical characteristics and 16S rRNA sequences is difficult. Molecular identification was used to discover suitable genes for distinguishing the two bacteria, and to identify the bio-controlling strain B29, due to molecular identification has been paid more and more attention. The similarity of four genes, cheA, gyrB, groEL and phoR, of the two species was compared by the sof...
Source: Cellular and Molecular Biology - July 20, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Label free quantitative proteomics analysis on the cisplatin resistance in ovarian cancer cells.
This study lays a foundation for deciphering the mechanism for drug resistance in ovarian tumor. PMID: 28719341 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - July 20, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

A pilot study for determination of anxiety related SLC6A4 promoter "S" and "L" alleles in healthy Turkish athletes.
A pilot study for determination of anxiety related SLC6A4 promoter "S" and "L" alleles in healthy Turkish athletes. Cell Mol Biol (Noisy-le-grand). 2017 May 20;63(5):29-31 Authors: Corak A, Kapici S, Sercan C, Akkoç O, Ulucan K Abstract We aimed to analyze the allelic distribution of solute carrier family-6 member-4 promoter region in Turkish athletes. Recent studies showed the association of lesser expressing "S" allele with anxiety. Genotype percentages for LL, LS and SS genotypes were found as 46, 35 and 19, respectively. 38% of the males had LL, %38 had LS and 2...
Source: Cellular and Molecular Biology - July 20, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Preparation and properties of polyvinyl alcohol (PVA) and hydroxylapatite (HA) hydrogels for cartilage tissue engineering.
Authors: Yuan F, Ma M, Lu L, Pan Z, Zhou W, Cai J, Luo S, Zeng W, Yin F Abstract A novel bioactive hydrogel for cartilage tissue based on polyvinyl alcohol (PVA) and hydroxylapatite (HA) were prepared, the effects of its component contents on the mechanical properties and microstructure of the hydrogel were investigated. The important properties of the scaffold composites, such as density, porosity, compressive modulus and microstructure were studied and analyzed through various measurements and methods. The biodegradability of hydrogel was evaluated by soaking the samples into artificial degradation solution at bo...
Source: Cellular and Molecular Biology - July 20, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Protective Effect of Edaravone Against A β25-35-Induced Mitochondrial Oxidative Damage in SH-SY5Y Cells.
Protective Effect of Edaravone Against Aβ25-35-Induced Mitochondrial Oxidative Damage in SH-SY5Y Cells. Cell Mol Biol (Noisy-le-grand). 2017 May 20;63(5):36-42 Authors: Zhang GL, Zhang L, Guo YY, Ma ZL, Wang HY, Li T, Liu J, Du Y, Yao L, Li TT, Du JM Abstract Amyloid-β (Aβ)-induced oxidative stress plays an important role in the pathogenesis of Alzheimer's disease (AD). Recent studies show that Aβ accumulation may lead to mitochondrial oxidative damage. In the present study, we investigated the protective effect of edaravone on mitochondrial damage in SH-SY5Y cells treated with A&be...
Source: Cellular and Molecular Biology - July 20, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

H2O2-induced mild stress in relation with in vitro ovine oocyte developmental competence: implications for blastocyst apoptosis and related genes expression.
In this study, in vitro maturation was performed in presence of various concentrations (0, 10, 100, or 1000 µM) of H2O2. The intracellular glutathione (GSH) level, fertilization, cleavage, and blastocyst rates, total cell number, and apoptotic cell number and expression of Bax, Bcl-2, and p53 genes in blastocyst-stage embryos were studied. At 10 μM H2O2 concentration, a higher GSH level was detected in comparison to the other groups while oocytes exposed to 1000 μM H2O2 had the lowest GSH level. Treatment of oocytes with 1000 μM H2O2 decreased the rate of two pronuclei formation as compared with other groups...
Source: Cellular and Molecular Biology - July 20, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Virulence genes in biofilm producer Enterococcus faecalis isolates from root canal infections.
This study aimed to evaluate the presence of E. faecalis virulence genes in dental root canal isolates recovered from advanced chronic periodontitis patients. One hundred E. faecalis isolated from dental root canal during July 2015 to Oct 2016. After analysis of biofilm formation by the semi-quantitative determination in 96-well flat bottom polystyrene plates, the presence of asa, esp, efaA, ace, ebpR, gel and hyl gene were studied by PCR. Gelatinase and hemolytic activity were detected by phenotypic methods. Ninety-one percent of isolates had ebpR gene, 85% ace, 82% efaA, 81% gel, 56% esp, 33% asa1, 2% hyl and 0% cyl gene...
Source: Cellular and Molecular Biology - July 20, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Regulatory effects of the novel synthesized Indole-3-carbaldehyde on expression of cell cycle genes: A study on Cyclin D and P21 expression by acute promylocytic leukemia cell line (NB4).
Authors: Karimabad MN, Mahmoodi M, Jafarzadeh A, Darekordi A, Hajizadeh MR, Khorramdelazad H, Sayadi AR, Khanamani Falahati-Pour S, Hassanshahi G Abstract A large variety of heterocycles are known to date and among these, indole and pyran rings are of particular interest. The present investigation was aimed to investigate the effects of novel Indole-3-carbaldehyde derivative (NI-3-CD) analogue on growth inhibition of cells and its regulatory effects on expression of cell cycle genes in acute promylocytic leukemia (APL) cell line. NB4 cells were cultured in presence of RPMI1640 medium contained various concentration...
Source: Cellular and Molecular Biology - July 20, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

TRIM59 is a key regulator of growth and migration inrenal cell carcinoma.
In this study, we aimed to investigate the role of TRIM59 in the cell growth and migration in RCC. The expression of TRIM59 in human RCC tissues was initially examined by qRT-PCR. Alentivirus-based shRNA against TRIM59 (Lv-shTRIM59) was constructed. The effects of TRIM59 knockdown on cell proliferation were examined by in vitro MTT assay, colony formation assay and in vivo a mouse xenograft model of RCC. Cell migration and invasion after knockdown of TRIM59 were also examined by transwell assay. Our data showed that the mRNA level of TRIM59 in cancerous tissues was 2-fold increased as compared with non-cancerous tissues. K...
Source: Cellular and Molecular Biology - July 20, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Identification of G2607A mutation in EGFR gene with a significative rate in Moroccan patients with Bladder Cancer.
In this study, we evaluated the frequency of point mutations in EGFR for future use of TKI in clinical treatment of bladder cancer. A total, 50 Moroccan patient specimens with bladder cancer and 48 healthy controls were analysed for EGFR mutations in the region delimiting exons 18-21 by PCR amplification and direct sequencing. Our results showed the absence of mutations in the EGFR kinase domain in these exons in all analysed specimens. However, sequence analysis of the EGFR-TK domain, revealed the presence of (G2607A) polymorphism at exon 20. Statistical analysis showed significant difference in the frequencies of G2607A ...
Source: Cellular and Molecular Biology - July 20, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Characterization of Candidate probionts isolated from human breast milk.
This study was designed to isolate and identify the potential probionts present in 32 healthy mothers' breast milk. Microbial culture media and 16SrRNA sequencing were used to isolate and identify the bacteria and all isolates were analyzed for their antagonistic potential, resistance to acidic pH, bile salts and survival under simulated gastric and intestinal conditions. The colonization potential was further assessed based on adherence to human enterocyte-like Caco-2 cell lines. The breast milk samples harbored significant numbers of Gram positive and catalase negative (85%) bacteria. Based on 16SrRNA sequencing, these i...
Source: Cellular and Molecular Biology - July 20, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

CCAT1 expressed in malignant and pre-malignant human gastric tissues.
Authors: Li X, Zhou Y, Qian H Abstract CCAT1 has been reported to implicate in various human malignancies. However, its expression in the oncogenesis of gastric cancer (GC) remains unknown. Quantitative reverse transcription (RT)-qPCR assay was performed to detect the expression of CCAT1 in 68cases of GC tissues,paired tumor-proximal gastric mucosa and normal gastric mucosa, 19 cases of gastric intraepithelial neoplasia (GIN), and the sera of these 68 GC patients and 22 healthy volunteers. And the relationship between CCAT1 and clinicopathologic features of GC patients were analyzed. Inthe present study, we found t...
Source: Cellular and Molecular Biology - July 20, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Determination of IL-6, TNF- α and VEGF levels in the serums of patients with colorectal cancer.
In this study, no significant difference was obtained between the mean scores concerning the IL-6 and VEGF serums of the colorectal cancer and healthy group (p>.05). But a statistically significant decrease was observed in the TNF-α serum level of the colorectal cancer group in comparison with the control group (p= .016; p < .05). PMID: 28719352 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - July 20, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effect of Triptolide on retinal ganglion cell survival in an optic nerve crush model.
In this study, we investigated the changes in retinal ganglion cell survival and axonal regeneration after administration of triptolide in optic nerve crush model. Triptolide treatment tended to promote retinal ganglion cell survival rather than optic nerve regeneration as well as inhibit the expression of tumor necrosis factor-α and activation of nuclear factor-kappa B. These findings suggested that intraperitoneal injection of triptolide may be an effective treatment for optic nerve injury and this effect was attributed at least in part to its anti-inflammatory actions. PMID: 28719353 [PubMed - in process] (Sou...
Source: Cellular and Molecular Biology - July 20, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Thymol and carvacrol strongly inhibit biofilm formation and growth of carbapenemase-producing Gram negative bacilli.
Authors: Raei P, Pourlak T, Memar MY, Alizadeh N, Aghamali M, Zeinalzadeh E, Asgharzadeh M, Kafil HS Abstract Discovery of novel drugs with new mechanisms of action and without cross-reaction with current therapeutic agents is crucial in the management of infections caused by multi-drug resistant (MDR) bacteria. The aim of the present study was to investigate effects of carvacrol and thymol on biofilm formation and antimicrobial activity against different carbapenemase-producing Gram negative bacilli. The antimicrobial and antibiofilm effect of thymol and carvacrol was investigated against strains harboring differe...
Source: Cellular and Molecular Biology - July 20, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

miRNA-223 suppresses FOXO1 and functions as a potential tumor marker in breast cancer.
In conclusion, we demonstrated that the miRNA-223can maintain cell proliferation of breast cancer cell through targeting FOXO 1, these results provide a new insight in tumor marker and potential therapeutic targets for breast cancer. PMID: 28719355 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - July 20, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Co-incubation of PMN and CaCo-2 cells modulates inflammatory potential.
We examined interactions of PMN and intestinal epithelial cell-like CaCo-2 cells to elucidate their regulation of inflammatory signalling and the impact of cyclooxygenase (COX), nitric oxide (NO) and platelet-activating factor (PAF). Human PMN and CaCo-2 cells, separately and in co-incubation, were stimulated with the calcium ionophore A23187 or with N-Formyl-methionyl-leucyl-phenylalanin (fMLP) that activates PMN only. Human neutrophil elastase (HNE) and respiratory Burst were measured. To evaluate the modulation of inflammatory crosstalk we applied inhibitors of COX (acetyl salicylic acid; ASA), NO-synthase (N-monomethyl...
Source: Cellular and Molecular Biology - July 20, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Vitamin D receptor BsmI polymorphism may be associated with an decreased osteoporosis risk in South China.
Authors: An M, Song XB, Chen XY Abstract Although many epidemiology studies have evaluated the correlation between vitamin D receptor (VDR) BsmI polymorphism and osteoporosis, the current results remain inconclusive. This meta-analysis updated and reevaluated the possible association between VDR BsmI polymorphism and osteoporosis in Chinese populations. Studies were identified using PubMed and Chinese databases through December 2016. The associations were assessed with pooled odds ratios (ORs) and 95% confidence intervals (CIs). A total of nine studies with 688 osteoporosis cases and 730 controls were included in t...
Source: Cellular and Molecular Biology - July 20, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The association between the UBQLN1 polymorphism and Alzheimer's disease risk: A systematic review.
Authors: Li X, Zhou J, Chen H, Wang F, Mei Q, Sun H Abstract Recently, some studies suggested that UBQLN1 variant was associated with AD risk. However, the results were inconsistent. This meta-analysis aimed to determine the association between UBQLN1 variant and AD risk. We searched the electronic databases PubMed, Embase, and CNKI databases. Random-effects model was used. All statistical tests were performed using the STATA 11.0 software (StataCorp, College Station, TX, USA). UBQLN1 variant was not associated with the risk of AD (OR=1.05; 95%CI, 0.92-1.19; I2=35%). The corresponding pooled ORs were not materially...
Source: Cellular and Molecular Biology - July 20, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Cell biology, biophysics, and mechanobiology: From the basics to Clinics.
Authors: Zeng Y Abstract Cell biology, biomechanics and biophysics are the key subjects that guide our understanding in diverse areas of tissue growth, development, remodeling and homeostasis. Novel discoveries such as molecular mechanism, and mechanobiological mechanism in cell biology, biomechanics and biophysics play essential roles in our understanding of the pathogenesis of various human diseases, as well as in designing the treatment of these diseases. In addition, studies in these areas will also facilitate early diagnostics of human diseases, such as cardiovascular diseases and cancer. In this special issue...
Source: Cellular and Molecular Biology - May 11, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Platelet-derived miR-142-3p induces apoptosis of endothelial cells in hypertension.
Authors: Bao H, Yao QP, Huang K, Chen XH, Han Y, Jiang ZL, Gao LZ, Qi YX Abstract The dysfunction of endothelial cells (ECs) plays crucial roles in vascular remodeling during hypertension. Researches suggested that ECs are regulated by the circulating platelets in vivo, which may participate in abnormal EC apoptosis in hypertension. However the molecular mechanism in this process is still unclear. Here we focused on the microRNAs (miRs) in platelets, and detected the potential role and delivery mechanism of platelet-derived miRs in ECs. Using microarray, the differentially expressed profile of miRs between platelet...
Source: Cellular and Molecular Biology - May 11, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effect of matrix rigidity on organ-specific capture of tumor cells by flow.
Authors: Ma P, Shen X, Tan P, Yang L, Liu W Abstract Accumulating evidences have suggested that tumor metastasis exists prominent organ discrepancy. In this progression, the capture of intravascular tumor cells (TCs) to endothelium in distant tissues and organs plays a decisive role in the organ-specific metastasis formation. However, the mechanism of tumor cells preferentially arrest and adhere to endothelial cells (ECs) of target organ still remains elusive. By using the parallel plate flow chamber and the polyacrylamide gels with different matrix stiffness, we here explored the combined effects of matrix ri...
Source: Cellular and Molecular Biology - May 11, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Sphingosine-1-phosphate reduces adhesion of malignant mammary tumor cells MDA-MB-231 to microvessel walls by protecting endothelial surface glycocalyx.
Authors: Zhang L, Zeng M, Fu BM Abstract Sphingosine-1-phosphate (S1P) is a sphingolipid in plasma that plays a critical role in cardiovascular and immune systems. Endothelial surface glycocalyx (ESG) decorating the inner wall of blood vessels is a regulator of multiple vascular functions. To test the hypothesis that S1P can reduce tumor cell adhesion to microvessel walls by protecting the ESG, we quantified the ESG and MDA-MB-231 tumor cell adhesion in the presence and absence of 1μM S1P, and in the presence of the matrix metalloproteinase (MMP) inhibitor in post-capillary venules of rat mesentery. We also meas...
Source: Cellular and Molecular Biology - May 11, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Role of microRNA-124 in cardiomyocyte hypertrophy inducedby angiotensin II.
In conclusion, our study shows that inhibition of miR-124 effectively suppresses AngII-induced myocardial hypertrophy, which is associated with attenuation of ER stress. PMID: 28478799 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - May 11, 2017 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research