Serum levels of IL-31, IL-33 and ST2 in allergic rhinitis of children in China.
This study aimed to compare the serum levels of IL-31, IL-33 and ST2 in children patients with allergic rhinitis (AR) and allergic asthma and tried to reveal more insights in development of AR. Thirty-six children patients with intermittent AR were selected in the present study during January 2013 to December 2016. Two control groups were set, the allergic asthma group and the healthy individuals. Atopic status of all participants in this study was tested and confirmed by skin prick testing (SPT). Levels of IL-31, IL-33 and ST2 were determined using ELISA. Statistical analysis was performed by SPSS 18.0. There is no signif...
Source: Cellular and Molecular Biology - October 12, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effects of melatonin on apoptosis and cell differentiation in MCF-7 derived cancer stem cells.
In this study, we investigated the effect of melatonin on apoptosis, self-renewal, and differentiation. For this purpose, MCF-7 and HEK293 cells were subjected to melatonin treatment. Expression of genes related to apoptosis (Bax and Bcl2) and self-renewal and differentiation (Oct4, Sox2, and Nanog) analyzed after the sorting of cancer stem cells from MCF-7 cells. Results showed that the effect of melatonin is dependent on the melatonin concentration and treatment periods. Melatonin treatment decreased the cell proliferation rate of MCF-7 in contrast to HEK293. Also, this treatment increased apoptosis in MCF-7 cells and de...
Source: Cellular and Molecular Biology - October 12, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effect of cobalt nanoparticles and cobalt ions on alkaloids production and expression of CrMPK3 gene in Catharanthus roseus suspension cultures.
Authors: Fouad AS, Hafez RM Abstract The capabilities of cobalt ions (Co2+) and cobalt nanoparticles (CoNPs) in enhancing alkaloids accumulation in Catharanthus roseus suspension cultures were evaluated in relation to the expression of CrMPK3 gene. Four concentrations (5, 10, 15 and 20 mg/L) of each cobalt form were utilized in addition to control. Both forms induced oxidative stress that was more pronounced for Co2+ treatments. A positive correlation was observed between cobalt concentration and expression of CrMPK3 gene. However, a characteristic temporal expression profile was recorded for each cobalt form. Also...
Source: Cellular and Molecular Biology - October 12, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

SMAR1 promotes immune escape of Tri-negative Breast Cancer through a mechanism involving T-bet/PD-1 Axis.
This study was aimed at investigating the specific molecular mechanisms involved in the regulation of immune escape of triple-negative breast cancer (TNBC) by SMRI, so as to provide a new clinical treatment target for the disease. Mouse original 4T1 breast cancer cells were inoculated subcutaneously in BALB/C to establish TNBC mouse model. CD8+T cells with immunological effects were selected from mouse thymus glands for primary culture. The CD8+positive T cells were infected with lentivirus interference vectors, and the proliferation of CD8+ T cells were determined by trypan blue staining and flow cytometry. CD8+T cells an...
Source: Cellular and Molecular Biology - October 12, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Characterization of antioxidant activity of sulfur compounds in black garlic.
Authors: Wang WD, Sun YE, Chen HW Abstract Different types of sulfur compounds, namely S-allyl-L-cysteine (SAC), S-allyl-L-cysteine sulfoxide (ACSO) and a synthetic γ-L-glutamyl-S-allyl-L-cysteine (GSAC) were extracted from black garlic and their inhibition to the advanced glycation end-products (AGEs) were investigated. Upon addition of inhibitor, the amount of produced fructosamine was determined by UV visible spectroscopy. The change of pentosidine and fluorescent AGEs during reaction was detected by fluorescence method and the change of carboxymethyl lysine (CML) was detected by high performance liquid ch...
Source: Cellular and Molecular Biology - October 12, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Omentin Val/Val genotype increases predisposition to acne vulgaris without changing omentin serum level.
In conclusion, omentin Val/Val (mutant homozygote) genotype increases predisposition to acne vulgaris by probably disrupting overall protein function of omentin. PMID: 30301508 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - October 12, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The effect of 6-week zinc supplement and weight training on the blood lipids of the sedentaries and athletes.
This study identifies that the values of the total cholesterol, LDL cholesterol and Triglyceride of the groups which were supplied with zinc supplement decreased, whereas the levels of HDL cholesterol increased in these groups. Consequently, this work demonstrates that the 6-weeks zinc supplement and training can make a significant contribution to the performance by changing positively the levels of blood lipid. PMID: 30213281 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - September 15, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Insecticidal effects of the Olea europaea subsp. laperrinei extracts on the flour Pyralid Ephestia kuehniella.
Authors: Lahcene S, Taibi F, Mestar N, Ali Ahmed S, Boumendjel M, Ouafi S, Houali K Abstract In the present study, the aerial parts of the Laperrine olive (Olea europaea subsp. Laperrinei) are subjected to acid extraction and the chemical composition of the extracts is determined by HPLC-DAD. The main compounds found in all of extracts are: hydroxytyrosol (30.45%), tyrosol (0.69%), oleuropein (32.76%), ferrulic acid (17.77%), quercetin (31.57%) and hesperetin (6.90%).The extracts obtained from the leafy stems of Laperrine olive tree are tested on the moth Ephestia kuehniella flour. Their administration by inhalatio...
Source: Cellular and Molecular Biology - September 15, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Determination of inhibitory activities of enzymes, related to Alzheimer's disease and diabetes mellitus of plane tree (Platanus orientalis L.) extracts and their antioxidant, antimicrobial and anticancer activities.
Authors: Ucar E, Eruygur N, Atas M, Ergul M, Ergul M, Sozmen F Abstract Plane tree (Platanus orientalis L.) leaves have been employed for centuries in various countries due to their pharmacological value. Therefore, determination of the biological activity of the leaves is of interest. The aim of the study was to evaluate the inhibitory effects against Alzheimer's disease-related enzymes Acetylcholinesterase (AChE) and Butyrylcholinesterase (BuChE), diabetes mellitus related enzymes α-glucosidase and α-amylase. The antioxidant, anticancer, and antimicrobial activities of the leaves were also studied. Ac...
Source: Cellular and Molecular Biology - September 15, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Genetic variation of yellow pistachio hard scale, Lepidosaphes pistaciae Archangelskaya (Hem.: Diaspididae) populations in Kerman province, Iran revealed by ISSR markers.
Authors: Rouhani M, Vahedi HA, Marefat A Abstract Yellow pistachio hard scale, Lepidosaphes pistaciae (Hem.: Coccoidea: Diaspididae) is one of the detrimental pests to pistachio trees. This pest is distributed throughout the pistachio producing regions of Iran. It is complex species, having distinct genetic variation. As genetically diversity awareness is essential for identification and management, the diaspidid samples selected from 10 infected region and used to test hypotheses about the genetic variability between and within its populations, during 2016. Inter simple sequence repeat (ISSR) molecular marker was ...
Source: Cellular and Molecular Biology - September 15, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Lentiviral-mediated BCL2 gene knockdown using comparative microRNA adaptive shRNAs.
In this study we will optimize lentiviral-mediated RNA interference (RNAi), recombinant lentiviruses accommodating anti-BCL2 micro adaptive short hairpin RNAs (shRNAs), to downregulate BCL2 in human embryonic kidney 293T (HEK293T) cells to produce stable cell lines. We tested 4 different Dharmacon™ GIPZ™ shRNAmir lentiviral vectors targeting BCL2 in different positions and a pGIPZ non-silencing shRNAmir lentiviral vector (as a negative control). Lentivirus packaging was performed by the calcium phosphate precipitation method. HEK293T cells were transduced by each type of recombinant lentiviruses individual...
Source: Cellular and Molecular Biology - September 15, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

MiR-33b inhibits osteosarcoma cell proliferation through suppression of glycolysis by targeting Lactate Dehydrogenase A (LDHA).
This study reveals that miR-33b plays a suppressive role in the regulation of osteosarcoma cell proliferation through direct targeting LDHA. The miR-33b/glycolysis/LDHA axis may contribute to development of therapeutic anti-tumor agents for osteosarcoma. PMID: 30213286 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - September 15, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Screening for novel peptides specifically binding to the surface of ectopic endometrium cells by phage display.
Authors: Linzhi Y, Dan C, Arhin SK, Ledan W, Wenju L, Jieqiang L Abstract A 7-mer phage display library was employed to isolate novel peptides that specifically bind to ectopic endometrium in vitro. Phage display technology with biopanning and rapid analysis of selective interactive ligands between ectopic and eutopic endometrium cells was utilized. After 5 rounds of biopanning, 50 phage clones were randomly selected and analyzed by enzyme-linked immunosorbent assay and DNA sequencing. A peptide-competitive inhibition assay was performed to identify the affinity of positive phages toward ectopic endometrium cells. ...
Source: Cellular and Molecular Biology - September 15, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Investigation of cochlear hair cells and the perception of ultrasound signals in guinea pigs.
Authors: Wang FS, Nie G, Huang CC, Li Q, Li T, Zou Y, Zhang S, Ceng X Abstract We established a specific ultrasound frequency-dependent model of cochlear injury using bone conduction ultrasounds in the inner ear of guinea pigs at 50 kHz and 83 kHz, to explore the effects of bone conduction ultrasound in the cochlea. To establish a unilateral cochlear damage model, the unilateral cochlea was destroyed. The control group consisted of 50 kHz and 83 kHz bone conduction ultrasounds in unaltered guinea pigs. In each group, cerebral blood oxygenation level dependent (BOLD) effects were determined by functional magnetic re...
Source: Cellular and Molecular Biology - September 15, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

MiR-637 suppresses melanoma progression through directly targeting P-REX2a and inhibiting PTEN/AKT signaling pathway.
In this study, we investigated the role of miR-637 in human melanoma and explored its relevant mechanisms. We found that the expression of miR-637 is significantly downregulated in melanoma tissues and cell lines. While overexpression of miR-637 inhibited melanoma cell proliferation and cell cycle G1-S transition, and induced apoptosis. Inhibition of miR-637 promoted cell proliferation and G1-S transition, and suppressed apoptosis. Subsequent investigation revealed that miR-637 expression was inversely correlated with P-REX2a expression in melanoma tissues. P-REX2a was determined to be a direct target of miR-637 by using a...
Source: Cellular and Molecular Biology - September 15, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effect of hesperetin on inflammatory and oxidative status in trinitrobenzene sulfonic acid-induced experimental colitis model.
This study indicates that hesperetin is protective against TNBS-induced colitis model via antiinflammatory, antioxidant and antiapoptotic effects. PMID: 30213290 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - September 15, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Expression profiles of proto-oncogene TWIST1 and tumor metastasis suppressor gene LASS2 in bladder cancer.
Authors: Yegin Z, Aydin O, Koc H, Buyukalpelli R Abstract Transcription factor proto-oncogene TWIST1 and tumor metastasis suppressor gene LASS2 have been reported to be involved in various carcinomas but their expression profiles and prognostic significances in bladder cancer are largely unknown. We aimed to determine these genes' expression levels both at mRNA and protein level in bladder cancer. mRNA expression levels of TWIST1 and LASS2 genes were examined using real-time quantitative PCR (qPCR) in human bladder tumors and paired normal adjacent tissues obtained from 44 patients. Protein expression profiles of b...
Source: Cellular and Molecular Biology - September 15, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The effects of apelin on myometrium contractions in pregnant rats.
Authors: Kacar E, Ercan Z, Serhatlioglu I, Sumer A, Kelestimur H, Kutlu S Abstract Apelin, which is a new hormone, is secreted especially in the brain by hypothalamus as well as by many other organs like the stomach, fat tissue, and the heart. For apelin, whose possible effects on many bodily functions like the endocrine system, cardiovascular system and metabolic activities are still under investigation, the reproductive system is also an important target area. The purpose of the present study was to investigate the effects of plasma apelin levels in rats that were in diestrus, pregnancy and lactation periods, and...
Source: Cellular and Molecular Biology - September 15, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effect of GDF11 on proliferation and apoptosis of esophageal cancer cells.
This study was aimed at investigating the effect of growth differentiation factor 11 (GDF11) on the proliferation and apoptosis of esophageal cancer cells. Serum levels of GDF11 in esophageal cancer patients were determined with ELISA kits, and the correlation between serum GDF11 and pathological features of esophageal cancer were determined. The effect of recombinant GDF11 on the growth of esophageal cancer cells was measured by CCK6 method. In order to investigate the effect of recombinant GDF11 on the proliferation and apoptosis of esophageal cancer cells, the expression of apoptosis-promoting protein Bax and proliferat...
Source: Cellular and Molecular Biology - September 15, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

-174 G/C polymorphism of interleukin 6 gene is not significantly different in Turkish professional short and long distance runners.
Authors: Arica S, Solgun HA, Demirel Dengi AS, Bakmaz E, Akçay T, Kapici S, Sercan C, Ulucan K Abstract Interleukin-6 (IL-6) is a kind of multifunctional cytokine and involved in mediating muscle repair metabolism, and therefore athletic capacity.  Muscular and circulating IL-6 levels increase in response to physical exercise. Responsible gene coding for IL-6 has a functional polymorphism in its promoter region, -174 G/C (rs1800795). We aimed to analyze the association of G allele and GG genotype in Turkish professional athletes and compare the allelic and genotypic difference between short distance and...
Source: Cellular and Molecular Biology - September 15, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Contrast enhanced ultrasound in diagnosis of endometrial carcinoma and endometrial hyperplasia.
Authors: Su Q, Sun Z, Lv G Abstract The present study was aimed to compare application of contrast enhanced ultrasound (CEUS) in diagnosis of endometrial carcinoma (EC) and endometrial hyperplasia (EH). A total of 81 patients with EC and simple EH were selected in this study. Among all patients, 39 cases were diagnosed as EC and 42 cases were diagnosed with EH. All patients were diagnosed by CEUS examination. The diagnosis of EC and EH was also confirmed by endometrial biopsy. CEUS was conducted for all patients. Eendometrial thickness was measured and the mean arrival time, time-to-peak, enhancement time, arrival ...
Source: Cellular and Molecular Biology - September 15, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

CTLA-4 (+49A/G) and NOD2/CARD15 (N852S) polymorphisms with inflammatory bowel disease in Turkish patients.
In this study, we evaluated the frequency of the CTLA-4 (+49A/G) and NOD2/CARD15 (N852S) polymorphisms in 62 patients with CD, 76 patients with UC, and 152 healthy individuals. The CTLA-4 and NOD2/CARD15 variants, rs231775 and rs104895467, were genotyped by PCR followed by RFLP. The results for the patients and the control group were statistically analyzed. According to our results, the CTLA-4 gene +49A/G polymorphism AA genotype was prevalent in CD patients and controls (29% vs 40%); the AG (56% vs 51%) and GG (15% vs 9%) genotypes were also observed. The prevalence of the of AA, AG and GG genotypes for the +49A/G polymor...
Source: Cellular and Molecular Biology - September 15, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Piperlongumine  as anticancer agent: The story so far about killing many birds with one stone.
Piperlongumine as anticancer agent: The story so far about killing many birds with one stone. Cell Mol Biol (Noisy-le-grand). 2018 Aug 30;64(11):102-107 Authors: Farooqi AA, Attar R, Yaylim I, Qureshi MZ, Todorovska M, Karatoprak GŞ, Najafi S, Sabitaliyevich UY, Zhenisovna TG, De Sousa DP, Lin X Abstract Piperlongumine is a biologically and pharmacologically active constituent of the plant Piper longum. This compound is gradually gaining attention because of its ability to inhibit/prevent different cancers. Modern era of molecular oncology is incomplete without ground-breaking discoveries made in...
Source: Cellular and Molecular Biology - September 15, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The influence of dimethyl sulfoxide (DMSO) on metabolic activity and morphology of melanoma cell line WM-266-4.
Authors: Ferk P, Dariš B Abstract Dimethyl sulphoxide (DMSO) is widely used as a solvent in biomedical research, regularly in concentrations up to 1%. Nevertheless, little is known about the effect of different DMSO concentration on WM-266-4 metastatic melanoma cells, which are often used in melanoma research. Due to resistance of melanoma cells high concentrations of cytotoxic substances soluble in DMSO are used in vitro tests. Consequently, total DMSO concentration often exceeds 1%. The aim of our study was to test the metabolic activity and morphology of WM-266-4 cells exposed to selected DMSO concentrati...
Source: Cellular and Molecular Biology - September 15, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Association between BIM polymorphism and lung cancer outcomes: a meta-analysis.
In conclusion, our analysis confirmed that lung cancer patients harboring the BIM deletion have inferior survival and TKI responses. Examination of the novel biomarker BIM deletion in lung cancer patients, especially for the EGFR mutant cohort, could provide some prognostic utility. PMID: 30213299 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - September 15, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Conservation of stored food using plant's extracts. Effect of oregano (Origanum vulgaris) essential oils on the reproduction and development of flour moth (Ephestia kuehniella).
Authors: Taibi F, Boumendjel M, Moncef Z, Omar S, Taha K, Amel D, Safa A, Hassiba R, Hanène C, Nacira S, Amel B, Mahfoud M Abstract In the search for effective methods of biological control of stored products insect-pests, the essential oil of Origanum vulgaris was extracted using Clevenger apparatus and tested on reproduction and mortality of flour moth Ephestia kuehniella. Bio-insecticide works with a double action mechanism; administered by inhalation in pupae, essential oil affects their pupal development. It also disrupts the reproduction of exuviated adults by extending the preoviposition period and re...
Source: Cellular and Molecular Biology - August 9, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

In vitro and in vivo androgen regulation of Dendropanax morbiferus leaf extract on late-onset hypogonadism.
Authors: Jung MA, Oh KN, Choi EJ, Oh DR, Kim YJ, Bae D, Hong JA, Pan S, Kim KM, Kim DW, Choi CY Abstract Late-onset hypogonadism (LOH) is associated with advancing age and is caused by a deficiency in serum testosterone levels. The aim of this study was to examine the effect of a Dendropanax morbiferus H.Lév. leaf extract (DME) on LOH using TM3 cells and aging male rats as in vitro and in vivo models, respectively. The in vitro effects of DME on testosterone levels and 3β-hydroxysteroid dehydrogenase (3β-HSD) protein expression in TM3 cells were analyzed. In the in vivo experiments, DME was orally ...
Source: Cellular and Molecular Biology - August 9, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Anti-cancer activity of asiatic acid against human cholangiocarcinoma cells through inhibition of proliferation and induction of apoptosis.
This study was performed to evaluate the cytotoxic effects of asiatic acid on two human CCA cell lines (KKU-156 and KKU-213). Cell viability was determined by a sulforhodamine B (SRB) assay. Morphological changes of the cells were observed by microscopy. Cell apoptosis was detected by flow cytometry using annexin V and propidium iodide (PI) staining. Messenger RNA (mRNA) expression levels of BAX, BCL2 and Survivin/BIRC5 were analyzed by real-time polymerase chain reaction (PCR). It was found that asiatic acid efficiently suppressed CCA cellular viability via induction of apoptosis. In addition, the occurrence of asiatic ac...
Source: Cellular and Molecular Biology - August 9, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Evaluation of metabolic reactivity in macrophages from mice with chronic sodium arsenite intake and experimental carcinogenesis.
Authors: Palmieri MA, Tasat DR, Molinari BL Abstract  Arsenic is carcinogenic to human beings, and environmental exposure to arsenic is a public health issue that affects large populations around the world. Thus, studies are needed to determine the mode of action of arsenic and to prevent harmful effects that arise from arsenic intake. In particular, knowledge of the effects of arsenic exposure in individuals who are undergoing a carcinogenesis process is lacking. The present study was performed in mice to evaluate the effect of chronic As3+ administration on peritoneal and alveolar macrophages; the As3+ was a...
Source: Cellular and Molecular Biology - August 9, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The effect of Diazoxide on norepinephrine-induced cardiac hypertrophy, in vitro.
Authors: Guven C Abstract Cardiac hypertrophy is associated with mitochondrial dysfunctions, which leads to heart failure if sustained. The aim of present study is to test hypothesis whether activation of mitochondrial KATP channel (mitoKATP) by diazoxide improve mitochondrial membrane potential (MMP) and oxidative stress in an in vitro model of cardiac hypertrophy. Rat cardiomyocytes cell line (H9c2) was used to create four groups as control, diazoxide, hypertrophy, hypertrophy and diazoxide. Norepinephrine was used to induce hypertrophy. Diazoxide and norepinephrine were simultaneously administered. After 24 hour...
Source: Cellular and Molecular Biology - August 9, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Polymorphisms in proinflammatory cytokine genes, effect on gene expression and association with preterm delivery in Saudi females.
In conclusion, this study revealed that rs361525 polymorphism plays a role as a protective marker for PTB and the levels of IL-1α, IL-6, TNF- α can be used as predicative biomarkers for PTB I Saudi women. PMID: 30084795 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - August 9, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Analysis of genetic polymorphisms associated with intervertebral disc degeneration.
In this study, we investigated the link between various polymorphic variants of the vitamin D receptor (VDR), matrix metalloproteinase 2 (MMP2), and insulin like growth factor 1 receptor (IGF1R) genes and IVDD in patients with IVDD, in Turkey. We examined and genotyped 199 patients with IVDD and 197 healthy individuals. Genomic DNA was isolated from the peripheral blood leukocytes of all participants, and analyzed using real-time PCR. Via melting curve analysis, VDR, MMP2, and IGF1R polymorphism variant distributions were determined. The patients with IVDD showed higher frequencies of the VDR ApaI A allele genotype as comp...
Source: Cellular and Molecular Biology - August 9, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Enhanced photodynamic therapy by encapsulation of perfluorocarbon into PEGylated near-infared dyes.
Authors: Jiang K, Wang H, Zhang H, Hu Y, Wu J Abstract Near-Infrared (NIR) dyes, with improved tissue penetration, minimal invasiveness and high specificity, have gained great interests in diagnosing and treating tumors. However, the poor solubility in aqueous medium and low 1O2 quantum yields of NIR dyes restrict their application in PDT (photodynamic therapy) research. Herein, a novel nanosystem with modifying the NIR dyes and encapsulating perfluorocarbon is reported for improving the PDT effectiveness of NIR dyes. By adding the PEG2000-SH and the C13 carbon chain to a NIR representative dye IR780, the new forme...
Source: Cellular and Molecular Biology - August 9, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Leucine can modulate the expression of proteins related to protein degradation signalling under mTOR inhibition in C2C12 cells.
In conclusion, the present study shows that leucine can modulate degradation pathways even under inhibition of mTOR by rapamycin. PMID: 30084798 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - August 9, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Antimicrobial Effect of Garlic (Allium sativum) and Thyme (Zataria multiflora Boiss) Extracts on Some Food Borne Pathogens and Their Effect on Virulence Gene Expression.
In conclusion, garlic and thyme extracts can be used not only as a flavor, but also as potential antimicrobial agents against Gram-positive and negative bacteria. PMID: 30084799 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - August 9, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Sensitization of cisplatin resistant bladder tumor by combination of cisplatin treatment and co-culture of dendritic cells with apoptotic bladder cancer cells.
This study provides a dendritic cell-based vaccine strategy which might reduce the risk of tumor recurrence and improve the efficiency of anti-chemoresistance of bladder cancer. PMID: 30084800 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - August 9, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Association of a MiR-499 SNP and risk of congenital heart disease in a Chinese population.
In this study, we aimed to test whether MTR is a direct target of miR-499, and to estimate the associations between miR-499 polymorphisms and the risk of CHD in Chinese population. Gene polymorphisms were analyzed in 1615 subjects including 792 healthy controls and 823 CHD patients. The miR-499 SNP were genotyped and the associations between the SNP frequencies and CHD were assessed by computing odds ratios (ORs) and 95% confidence intervals (95% CIs), as well as by applying Chi-square tests. Dual reporter assay was carried out to test whether MTR is a direct target gene of miR-499. The miR-499 rs374644 AG genotype was not...
Source: Cellular and Molecular Biology - August 9, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Artesunate and chloroquine induce cytotoxic activity on cholangiocarcinoma cells via different cell death mechanisms.
In this study, we revisited the possibility of artesunate (ART) and chloroquine (CQ), the antimalarial drugs, as therapeutic agents against CCA. The possible mechanisms of these drugs to exert cytotoxicity on CCA cells were also explored. The effects of ART and CQ on proliferation and death patterns of two CCA cell lines, KKU-214 and its highly metastatic subtype KKU-214L5, were examined using water soluble tetrazolium (WST) assay and time-lapse photometry, respectively. To differentiate and verify the death patterns between necrosis and apoptosis, lactate dehydrogenase (LDH) release, and caspase 3 activity were measured. ...
Source: Cellular and Molecular Biology - August 9, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Pyridoxine exerts antioxidant effects in cell model of Alzheimer's disease via the Nrf-2/HO-1 pathway.
In this study, the anti-AD effects of pyridoxine were studied in an AD cell model using a combination of techniques viz MTT assay, western blotting and assays for reactive oxygen species (ROS). Assays were also carried out to determine the mechanism underlying the antioxidant effects of pyridoxine. The results obtained revealed that pyridoxine exerted a protective potential against AD, attenuated ROS levels, decreased the expressions of cytoplasmic Nrf2, and upregulated whole-cell HO-1 expression. These results suggest that the anti-AD effect of pyridoxine may be attributed to its anti-oxidant property elicited via stimula...
Source: Cellular and Molecular Biology - August 9, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Warning: spread of NDM-1 in two border towns of Iran.
This study aimed to detect the metallo-beta-lactamase in the isolated gram-negative bacteria of the Iranian clinical specimens collected from two major cities in Iran. In this cross sectional study 171 Acinetobacter baumannii, 120 Enterobacter spp. and 145 Klebsiella pneumoniae isolated from clinical specimens of two training hospitals in Tabriz and Mashhad were evaluated. Carbapenem resistant screening was performed according to CLSI guide line. The antibiotic susceptibility testing was prepared for carbapenem resistant strains. Then, the metallo-beta- lactamase genes detection was also carried out by PCR assay and confir...
Source: Cellular and Molecular Biology - August 9, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Nesfatin-1 and irisin levels in response to the soccer matches performed in morning, afternoon and at night in young trained male subjects.
This study aimed to investigate the potential effects of acute soccer matches performed in morning, afternoon and at night on both nesfatin-1 and irisin levels in trained subjects. Total of 20 male subjects performed in soccer matches at three different times of day: morning, afternoon, and night. Pre- and post-match venous blood samples were taken, and levels of both nesfatin-1 and irisin were analysed using the enzyme-linked immunosorbent assay (ELISA). Following all matches, the subjects' irisin levels increased significantly in all subjects (p < 0.0001). Nesfatin-1 levels were also increased after the matches; h...
Source: Cellular and Molecular Biology - August 9, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Chlorogenic-induced inhibition of non-small cancer cells occurs through regulation of histone deacetylase 6.
Authors: Hongtao L, Xiaoqi G, Junni L, Feng X, Guodong B, Liang Y Abstract Chlorogenic acid (CGA), an ester with various pharmacological effects, is important in cancer therapy. However, the specific antitumor mechanism of CGA is not entirely clear, especially with respect to its suppression of non-small cell lung cancer (NSCLC). The present study was carried out to assess the effect of CGA on NSCLC, and the mechanism involved. Cell viability assay and colony formation assay revealed that CGA blocked the proliferative capacity of NSCLC cells in vitro. Results from the migration assay suggested that CGA also inhibit...
Source: Cellular and Molecular Biology - August 9, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Sinomenine relieves oxygen and glucose deprivation-induced microglial activation via inhibition of the SP1/miRNA-183-5p/I κB-α signaling pathway.
In conclusion, this study shows that sinomenine effectively inhibits OGD/R-induced inflammatory activation in MG by suppressing the activation of transcription specificity protein 1 (SP 1). This finding is of significance for the clinical use of sinomenine in treating cerebral ischemia/reperfusion injury. PMID: 30084807 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - August 9, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Maslinic acid as an effective anticancer agent.
Authors: Lin X, Ozbey U, Sabitaliyevich UY, Attar R, Ozcelik B, Zhang Y, Guo M, Liu M, Alhewairini SS, Farooqi AA Abstract Maslinic acid (2α,3β-dihydroxyolean-12-en-28-oic acid) is a naturally occurring pentacyclic triterpenic compound. Maslinic acid is gradually gaining attention as an excellent pharmacologically active product because of its premium biological properties. In this review we will focus on chemopreventive properties of Maslinic acid against different cancers. Seemingly, available data is limited and we have yet to unravel how Maslinic acid therapeutically targeted oncogenic cell signal tr...
Source: Cellular and Molecular Biology - August 9, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Stem cell therapy for lung diseases: From fundamental aspects to clinical applications.
Authors: Hosseinirad H, Rashidi M, Moghaddam MM, Tebyanian H, Nouraei S, Mirhosseini SA, Rasouli M, Habibian S Abstract The respiratory system is a complex group of organs in the human body, all of which are necessary in breathing. Due to its special anatomy and composition, after exposure to various damaging factors such as micro particles, carbon granules and toxic gases, the respiratory system can be affected by a variety of damage without return to its original state. Currently, the prevalence of lung diseases, including asthma, and chronic obstructive pulmonary diseases, such as emphysema, has increased remark...
Source: Cellular and Molecular Biology - August 9, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Role of statin as inducer of Hmox-1 system in treatment of preeclampsia.
Authors: Putra RA, Effendi JS, Permadi W, Bandiara R, Fauziah PN Abstract Preeclampsia is the major cause of both maternal and neonatal morbidity and mortality. Its insidence remains high and the management has not been established yet because its etiology and pathophysiological are still poorly understood. Theories regarding etiopathogenesis and management of preeclampsia have been postulated yet it remains controversial. Placental ischemic and angiogenic imbalance are suggested to be predisposing factors of preeclampsia. It is thereby targeted in prevention of preeclampsia. Unfortunately, both primary and seconda...
Source: Cellular and Molecular Biology - August 9, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Energy requirements in mammalian oogenesis.
Authors: Arhin SK, Lu J, Xi H, Jin X Abstract Oogenesis is a lengthy, multi-step process occurring in mammals yielding single or multiple oocytes capable of being fertilized upon interaction with male gametes. The overall process is highly complex in nature, starting in the primordial follicles, and its ultimate completion is preceded by the meiotic cycle. There are two major phases in oogenesis: the growth phase, followed by a maturation phase that requires relatively less time. Both phases require energy for the various metabolic processes of the oocytes. The energy requirements and the timing of maturation vary ...
Source: Cellular and Molecular Biology - August 9, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Meta-analysis of gene expression profiles of peripheral blood cells in systemic lupus erythematosus.
Authors: Bae SC, Lee YH Abstract Our purpose was to identify differentially expressed (DE) genes and biological processes associated with gene expression changes in systemic lupus erythematosus (SLE). We performed a meta-analysis using the INMEX program (integrative meta-analysis of expression data) on publicly available microarray Genetic Expression Omnibus (GEO) datasets of peripheral blood mononuclear cells (PBMCs) of SLE patients and healthy controls. We performed Gene Ontology (GO) enrichment analysis by using hypergeometric tests. In total, five comparisons (2 B cells, 2 CD4 T cells, and 1 myeloid cell) from ...
Source: Cellular and Molecular Biology - August 9, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Influence of abiotic elicitors on improvement production of artemisinin in cell culture of Artemisia annua L.
Authors: Zebarjadi A, Dianatkhah S, Pour Mohammadi P, Qaderi A Abstract A significant sesquiterpene lactone used as a drug is artemisinin. It is definitely an anti-parasitic drug isolated from field-grown Artemisia annua L. a plant from Asteraceae family. It is the best treatment for Plasmodium falciparum malaria. Unfortunately, artemisinin content in A. annua is extremely low (0.01-0.8% dry weight). So, some researchers focused on enhancing artemisinin content either in tissue/cell culture or the whole plant of A. annua sp. The aims of the current study were the effect of plant growth regulators on callus producti...
Source: Cellular and Molecular Biology - July 23, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Resistant/susceptible classification of respiratory tract pathogenic bacteria based on volatile organic compounds profiling.
This study demonstrated that resistant strains of bacteria produced VOCs that were different from those of the standard strains. In addition, VOCs released by bacteria after passing the logarithmic growth phase showed no significant differences. The identification of VOCs can be a precise way to differentiate bacterial species, also it can be said that the VOCs produced by different pathogenic microorganisms can be the suitable biomarkers for their detection. PMID: 30030949 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - July 23, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research