ATF3/EGR1 regulates myocardial ischemia/reperfusion injury induced autophagy and inflammation in cardiomyocytes
Cell Mol Biol (Noisy-le-grand). 2024 Mar 31;70(3):125-129. doi: 10.14715/cmb/2024.70.3.18.ABSTRACTMyocardial ischemia/reperfusion injury (MIRI) is an irreversible adverse event during the management of coronary heart disease that lacks effective controls. The underlying mechanism of MIRI still requires further investigation. Recent studies have suggested that overexpression of ATF3 protects against MIRI by regulating inflammatory responses, ferroptosis, and autophagy. The downstream target of ATF3, EGR1, also showed cardioprotective properties against MIRI by promoting autophagy. Therefore, further investigating the effect...
Source: Cellular and Molecular Biology - April 23, 2024 Category: Molecular Biology Authors: Li Li Gang Fu Caiyun Liu Yanling Liu Source Type: research
Knockdown of growth differentiation factor-15 restrains prostate cancer through regulating MAPK/ERK signaling pathway
This study investigates the regulatory function of GDF15 in prostate cancer. To explore GDF15's impact, we established GDF15 knockdown and overexpression models in prostate cancer cells. We quantified mRNA and protein levels using RT-PCR and Western blotting. Functional assays, including CCK8, Transwell, wound healing, and flow cytometry, were employed to evaluate cell proliferation, invasion, migration, and apoptosis. Additionally, the effect of GDF15 on tumor growth was assessed using a metastatic tumor model in nude mice. Elevated GDF15 expression was identified in prostate cancer tissues and cells. The knockdown of GDF...
Source: Cellular and Molecular Biology - April 23, 2024 Category: Molecular Biology Authors: Minggen Yang Miaolin Guo Chenqiang Su Weiping Hao Zhenqiang Xu Source Type: research
Identification of core genes of craniopharyngioma angiogenesis based on single-cell nuclear transcriptome sequencing
This study aimed to explore the core genes of craniopharyngioma angiogenesis for targeted vascular therapy based on single-cell nuclear transcriptome sequencing. For single-cell nuclear transcriptome sequencing, we collected six samples from the tumor center and adjacent hypothalamic tumor tissues from three patients with craniopharyngioma, as well as four normal brain tissues based on Gene Expression Omnibus. We screened genes with differential up-regulation between vascular endothelial cells of craniopharyngioma and those of normal brain tissues, performed GO and KEGG analysis, constructed the protein-protein interaction...
Source: Cellular and Molecular Biology - April 23, 2024 Category: Molecular Biology Authors: Jinshi Zhang Lin Xu Jiye Ye Chunming Xu Bowen Wu Jie Wu Tao Hong Source Type: research
Comprehensive bioinformatics analysis of KIF20A as a prognosis biomarker for clear cell renal cell carcinoma
Cell Mol Biol (Noisy-le-grand). 2024 Mar 31;70(3):116-124. doi: 10.14715/cmb/2024.70.3.17.ABSTRACTIt has been shown that kinesin family member 20A (KIF20A) is involved in the development of several cancers. However, research on clear cell renal cell carcinoma (ccRCC) and KIF20A is still exploratory. The current research was carried out to determine whether KIF20A expression has any prognosis value in ccRCC. Data were downloaded from The Cancer Genome Atlas (TCGA) database to validate the KIF20A mRNA expression and to perform clinicopathological analysis. Receiver operating characteristic (ROC) curves were used in evaluatin...
Source: Cellular and Molecular Biology - April 23, 2024 Category: Molecular Biology Authors: Cankun Xie Wingkeung Yiu Yijiang Mo Source Type: research
Chelerythrine induces apoptosis and ferroptosis through Nrf2 in ovarian cancer cells
In this study, we investigated the cytotoxic mechanism and potential targets of CHE on in vitro cultures of A2780 and SKOV3 cells derived from ovarian cancer. Additionally, in vivo experiments were conducted to confirm the suppressive impact of CHE on tumor growth in nude mice. The findings revealed that CHE impeded the growth of A2780 and SKOV3 cells in a concentration-time-dependent manner and significantly suppressed the development of tumors in nude mice. CHE elevated the level of oxidative stress in tumor cells, prompted cell cycle halt in the S phase, and increased their mitochondrial membrane potential. Western blot...
Source: Cellular and Molecular Biology - April 23, 2024 Category: Molecular Biology Authors: Jia Zhou Yuchen Wang Yangxin Fu Zhe Lin He Lin Guangfu Lv Ning Kong Guo Xin Fang Zhou Zhidong Qiu Xiaowei Huang Source Type: research
Lactoferrin mediates epithelial-mesenchymal transformation by regulating the PI3K/AKT/mTOR pathway to inhibit nasopharyngeal carcinoma metastasis
This study suggests that LTF is a potential biomarker of NPC and that LTF-mediated EMT progression plays a tumor-suppressive role in the progression of NPC metastasis.PMID:38650146 | DOI:10.14715/cmb/2024.70.3.19 (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - April 23, 2024 Category: Molecular Biology Authors: Min Xu Ye Fan Guoying Zou Qi Yang Fei Xu Source Type: research
Development of a novel six DNA damage response-related prognostic signature in osteosarcoma
Cell Mol Biol (Noisy-le-grand). 2024 Mar 31;70(3):110-115. doi: 10.14715/cmb/2024.70.3.16.ABSTRACTDNA damage response (DDR) plays a vital role in the development of cancer. Nevertheless, in osteosarcoma, the potential of DDR-related genes (DDRGs) remains unclear. Thus, the current research is intended to investigate the mechanisms of DDRGs in the development of osteosarcoma and to explore potential DDR-related biomarkers in forecasting the prognosis of osteosarcoma patients. The osteosarcoma genomic data from TCGA, GEO and cBioPortal databases were utilized for screening and identification of differentially expressed DDRGs...
Source: Cellular and Molecular Biology - April 23, 2024 Category: Molecular Biology Authors: Tianyang Li Zhenzhou Tang Sucheng Li Minhua Lu Source Type: research
TNFRSF6 induces mitochondrial dysfunction and microglia activation in the in vivo and in vitro models of sepsis-associated encephalopathy
This study aimed to explore TNFRSF6 function in SAE. The SAE mouse model was established by intraperitoneal injection of LPS in TNFRSF6-/- mice and C57BL/6J mice. Microglia were treated with LPS to establish the cell model. The learning, memory and cognitive functions in mice were tested by behavioral tests. Nissl staining was utilized for determining neuronal injury. Microglial activation was tested by immunofluorescence assay. ELISA was utilized for determining TNF-α, IL-1β, IL-6, and IL-10 contents. Mitochondrial dysfunction was measured by mitochondrial oxygen consumption, ATP content, ROS production, and JC-1 assay....
Source: Cellular and Molecular Biology - April 23, 2024 Category: Molecular Biology Authors: Danfeng Yu Yanmei Ji Jing Zhang Xiaochuan Huang Source Type: research
Ginsenoside Rc alleviates osteoporosis by the TGF- β/Smad signaling pathway
Cell Mol Biol (Noisy-le-grand). 2024 Mar 31;70(3):95-101. doi: 10.14715/cmb/2024.70.3.14.ABSTRACTOsteoporosis is a common chronic bone disorder in postmenopausal women. Ginsenosides are primary active components in ginseng and the effects of various ginsenoside variants in osteoporosis treatment have been widely revealed. We planned to explore the impact of ginsenoside Rc on bone resorption in an osteoporosis rat model. We used ovariectomized rats to assess the potential impact of ginsenoside Rc on osteoporosis. μ-CT was implemented for analyzing the microstructure of the distal left femur in rats. H&E staining togeth...
Source: Cellular and Molecular Biology - April 23, 2024 Category: Molecular Biology Authors: Shanfu Wang Bing Xu Heng Yin Zhen Hua Yang Shao Jianwei Wang Source Type: research
Identification of osteogenic differentiation-related miRNA-mRNA regulatory network of human periodontal ligament stem cells
This study aimed to investigate the hub genes and miRNA-mRNA regulatory network around periodontal ligament stem cells (PDLSC) for osteogenic differentiation through bioinformatic analysis. The dataset with osteogenic differentiation of human PDLSC was downloaded from the GEO database. The Weighted gene coexpression network analysis (WGCNA) was performed to identify key modules and hub genes. In addition, differentially expressed genes (DEGs) analysis was conducted with limma. The functional enrichment of differentially expressed hub genes was implemented with KEGG and GSEA analysis. The targeted genes of differentially ex...
Source: Cellular and Molecular Biology - April 23, 2024 Category: Molecular Biology Authors: Zhenrong Chen Lulu Long Mianjia Wan Min Huang Haijuan Wu Qun Huang Source Type: research
Identification of 10 differentially expressed and cuproptosis-related genes in immune infiltration and prognosis of thyroid carcinoma
We presented the expression profiles of dysregulated genes in thyroid carcinoma. The findings of our study highlighted the potential of cuproptosis-related genes as prognostic biomarkers for thyroid carcinoma.PMID:38650151 | DOI:10.14715/cmb/2024.70.3.13 (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - April 23, 2024 Category: Molecular Biology Authors: Weixuan Yu Haiping Liu Yunda Zhang Ming Liu Weicong Li Libin Wang Dongwei Li Source Type: research
The relationship between preeclampsia risk and SENCR rs555172 gene polymorphism and expression
In conclusion, the LncRNA SENCR rs555172(G/A) seems not associated with an increased risk of Preeclampsia in pregnant women.PMID:38650152 | DOI:10.14715/cmb/2024.70.3.11 (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - April 23, 2024 Category: Molecular Biology Authors: Mohsen Saravani Elham Kazemi Hasan Dana Sepehr Kahrizi Roya Zanganeh Hamidreza Chegini Sodabe Rezaei Marzieh Ghasemi Majid Zaki-Dizaji Mostafa Saeedinia Zohreh Heidary Source Type: research
Antibacterial effect and biocompatibility of silver nanoparticle-coated bone allograft substitutes
This study aimed to coat bone allografts with silver nanoparticles and assess their antibacterial activity and biocompatibility compared to uncoated bone allografts. In this study, the bone allografts were coated with varying concentrations of silver nanoparticles (5 mg/l, 10 mg/l, and 50 mg/l) through a simple adsorption technique. Subsequently, the coated samples underwent characterization using SEM, FTIR, EDS, and XRD. The Minimal Inhibitory Concentration (MIC) of the silver nanoparticles was determined against Staphylococcus aureus and Streptococcus mutans. Bacterial growth inhibition was evaluated by measuring turbidi...
Source: Cellular and Molecular Biology - April 23, 2024 Category: Molecular Biology Authors: Sanna Hadi Othman Omar Source Type: research
MicroRNA-503-5p protects streptozotocin-induced erectile dysfunction in diabetic rats by downregulating SYDE2
This study aimed to figure out microRNA (miR)-503-5p/SYDE2 axis' latent mechanisms in streptozotocin-induced diabetic rat sexual dysfunction. A model of erectile dysfunction (ED) in diabetic rats was established by injecting streptozotocin. MiR-503-5p and SYDE2 in ED rats were altered by injection of miR-503-5p mimic or si/oe-SYDE2. The targeting link between miR-503-5p and SYDE2 was testified. ICP/MAP value was tested by pressure sensor; Penile capillary abundance was assessed; Penile cGMP and AGEs were detected; penile smooth muscle cell apoptosis was assessed; MiR-503-5p and SYDE2 were tested. In streptozotocin-induced ...
Source: Cellular and Molecular Biology - April 23, 2024 Category: Molecular Biology Authors: Gangqin Li Lin Jiang Kuan Bai Guangxing Tan Source Type: research
Identification of hub genes and key pathways in spinal cord injury via bioinformatics analysis
This study aimed to explore the hub genes and related key pathways in Spinal Cord Injury (SCI) based on the bioinformatics analysis. Two microarray datasets (GSE45006, GSE45550) were obtained from the GEO database and were merged and batch-corrected. The differentially expressed genes (DEGs) in SCI were explored with the Limma, and the weighted gene co-expression network analysis (WGCNA) was conducted to explore the module genes. Functional enrichment analysis and Gene set variation analysis (GSVA) were used to investigate the biological functions and key pathways of the key genes related to SCI. Then the protein-protein i...
Source: Cellular and Molecular Biology - April 23, 2024 Category: Molecular Biology Authors: Hao Cheng Yanben Wang Jun Xuan Source Type: research
