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Establishment of plasma microRNA detection method by using taqman probe based quantitative reverse transcription PCR.
In this study, we tried to establish a reliable method to quantify the plasma miRNAs. We used quantitative real—time PCR with taqman probes to detect the plasma miR—153 level. Three controls were used in this study, including two external miRNAs control from C. elegans miRNAs (cel—miR—54 and cel—miR—238) and one internal control (hsa—miR—486). All of these controls were stable in plasma and the cel—miR—238/cel—miR—54/hsa—miR—486 combination could improve the normalization process. The ...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effect of Lead stress on phosphatase activity and reducing power assay of Triticum aestivum.
Authors: Gubrelay U, Agnihotri RK, Shrotriya S, Sharma R Abstract Lead (Pb) is a highly toxic heavy metal for both plants and animals; the environment is increasingly polluted with heavy metals and reduces crop productivity. Plants possess homeostatic mechanisms that allow them to keep correct concentrations of essential metal ions in cellular compartments and to minimize the damaging effects of an excess of nonessential ones. One of their adverse effects on plants are the generation of harmful active oxygen species, leading to oxidative stress and the antioxidative activity seems to be of fundamental importance fo...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

AIM2 inflammasome is dispensable for the host defense against Pseudomonas aeruginosa infection.
In this study, we found that P. aeruginosa infection induced degradation of AIM2 protein in macrophages and induction of AIM2 mRNA expression in macrophages and in the lung of mice. Interestingly, P. aeruginosa infection induced a similar level of IL—1β, IL—6 and TNF production in wild—type and AIM2—deficient mice. Similarly, no significant differences in bacterial clearance, neutrophil infiltration and NF—κB activation were observed between wild—type and AIM2—deficient mice following P. aeruginosa lung infection. Our data suggest that AIM2 inf...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Cloning, purification and characterization of a thermostable β-galactosidase from Bacillus licheniformis strain KG9.
Cloning, purification and characterization of a thermostable β-galactosidase from Bacillus licheniformis strain KG9. Cell Mol Biol (Noisy-le-grand). 2015;61(3):71-8 Authors: Matpan Bekler F, Stougaard P, Güven K, Gül Güven R, Acer Ö Abstract A thermo— and alkalitolerant Bacillus licheniformis KG9 isolated from Taşlıdere hot water spring in Batman/Turkey was found to produce a thermostable β—galactosidase. Phylogenetic analysis showed that the 16S rRNA gene from B. licheniformis strain KG9 was 99.9% identical to that of the genome sequenced B. ...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

HOTAIR inhibited intracellular Ca2+ via regulation of Cav1.2 channel in human cardiomyocytes.
Authors: Tan WL, Xu M, Liu Z, Wu TY, Yang Y, Luo J, Yang J, Luo Y Abstract HOTAIR, a long non—coding RNA (lncRNA), is reported to regulate chromatin organization and promote tumor progression. However, little is known about the roles of this gene in the modulation of calcium homeostasis in human cardiomyocytes. In the present study, we demonstrated that up—regulation of HOTAIR could suppress the expression of CaV1.2 in human cardiomyocytes. However, HOTAIR knockdown promoted CaV1.2 expression in human cardiomyocytes. In addition, we found that HOTAIR overexpression significantly reduced the intr...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

MicroRNA profiling during germline differentiation of mouse embryonic stem cells.
Authors: Ebrahimzadeh-Vesal R, Shokrgozar MA, Nayernia K, Teimoori-Toolabi L, Estiar MA, Miryounesi M, Nourashrafeddin S, Modarressi MH Abstract MicroRNAs are new classes of small non—coding regulatory RNAs which control degradation or suppress translation of its target mRNAs by sequence complementarity. Mature microRNAs are enriched in embryonic stem cells and play important roles in controlling stem cell self—renewal as well as control of differentiation. There is significant evidence that microRNAs are involved in the regulation of stem cell differentiation. The male mouse Embryonic Stem Cell...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Human Toll like receptor 4 gene expression of PBMCs in diabetes mellitus type 2 patients.
In this study, we evaluate the expression of the TLR4 in patients with diabetes mellitus type 2 (DM2) in comparison to healthy controls. Expression of TLR4 in 32 human peripheral blood mononuclear cells (PBMCs) of patients with DM2 and 20 control samples was assessed using Real—Time PCR technique. For each patient, body mass index (BMI) and blood glucose levels were measured. The results of Real—Time PCR showed a 5—folds increase in expression of TLR4 on the PBMCs of DM2 patients in comparison to controls. No correlation was observed between the TLR4 expression and sex or BMI. Our results conf...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Angiotensin II Promotes Atherogenesis through upregulating the Expression of Connexin 43 in Dendritic Cells.
Authors: Nie W, Yan H, Li S, Zhu W, Fan F, Zhu J Abstract It is known, for a long time, that angiotensin II (Ang II) could contribute to atherogenesis (AS) and plaque vulnerability, however the underlying mechanisms are poorly understood. Dendritic cells (DCs) are critical for the development of both inflammation and atherogenesis. In the present study, we tried to investigate the influence of AngII on the expression of connexin43 (Cx43) in DCs, as well as the effect of AngII on AS. After mouse bone marrow—derived dendritic cells (BMDCs) were treated by Ang II with or without Valsartan, the expression of ...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Withaferin A attenuates lipopolysaccharide-induced acute lung injury in neonatal rats.
Authors: Gao S, Li H, Zhou XQ, You JB, Tu DN, Xia G, Jiang JX, Xin C Abstract Withaferin A (WFA) is an active compound from Withania somnifera and has been reported to exhibit a variety of pharmacological activities such as anti—inflammatory, immunomodulatory and anti—tumor properties. In the present study, we investigated the potential protective role of WFA on acute lung injury in neonatal rats induced by lipopolysaccharide (LPS). We found that WFA significantly attenuated the pathological changes of lungs induced by LPS injection. Administration with WFA obviously decreased pulmonary neutroph...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Different components of chicken ovalbumin extract promote different cell proliferation.
Authors: Ruan GP, Yao X, Liu JF, Wang JX, Pan XH Abstract The chicken ovalbumin extracts could promote cell survival and proliferation. In the present study, the different components in chicken ovalbumin extracts were further separated to find the component primarily responsible for promoting cell proliferation. Components of differing molecular weight were separated from chicken ovalbumin extracts by ultrafiltration. Different components were co—cultured with different cells at different final concentrations, and the effects on cell proliferation were subsequently determined by a CellTiter 96 Aqueous One...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

In Vitro and In Vivo investigations on fibronectin coated and hydroxyapatite incorporated scaffolds.
In conclusion, fabrication of nanofiberous scaffold with this combination of biochemical composition and surface stimulation caused improved biodegradability and biocompatibility of the scaffold which are desirable in more effective tissue regeneration. PMID: 26255261 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Protective effect of bioactive peptide carnosine against lead-induced oxidative stress in kidney of rats.
Authors: Hasanein P, Teimuri-Far M Abstract Oxidative stress is among the mechanisms involved in renal injury. We aimed to investigate the protective effects of bioactive peptide carnosine on lead induced oxidative stress and nephrotoxicity in rats. Animals received an aqueous solution of lead acetate (500 mg Pb/L in the drinking water) and/or carnosine (10 mg/kg, i.g.) for eight weeks. Then rats were weighed and used for biochemical, histological and oxidant/antioxidant evaluations. Lead—induced oxidative stress in renal tissue was indicated by a significant increase in the renal contents of malondialdeh...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Early intracellular signaling events induced by in vitro metreleptin administration in cardiac myocytes and uterine smooth muscle cells.
Authors: Choi SK, Park S, Choi Y, Moon HS Abstract Intracellular signaling pathways regulated by leptin have largely been studied in metabolically important organs such as adipose tissue and peripheral blood mononuclear cells, suggesting that leptin plays a key role in pathophysiology of insulin resistance. However, whether synthetic analog of leptin, metreleptin, has similar effects on cardiac myocytes (CM) and uterine smooth muscle cells (USMC) has not yet been studied. Hence, in order to address these questions, we extended previous observations and investigated in vitro signaling study whether metreleptin may a...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The association between 1349T>G polymorphism of ApE1 gene and the risk of prostate cancer in northern Iran.
The association between 1349T>G polymorphism of ApE1 gene and the risk of prostate cancer in northern Iran. Cell Mol Biol (Noisy-le-grand). 2015;61(4):21-4 Authors: Pournourali M, Tarang AR, Yousefi M Abstract Prostate cancer (PCa) is the most common malignancy in men and the fourth most common cause of death based on cancer all over the world. Many genes has been shown to be involved in the progress of the prostate cancer. Human apurinic/apyrimidinic endonuclease 1 (APE1) is a multifunctional protein that has an important role in the base excision repair (BER) pathway. The aim of this study wa...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Identification and expression analysis of 4-Coumarate: Coenzyme A ligase gene family in Dryopteris Fragrans.
In conclusion, we identified 4CLs genes in ferns D. fragrans and analyzed the expressions of these genes, and finally explored the relationship between the expressions of 4CLs and syntheses of flavonoids and lignin. PMID: 26255265 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Long non-coding RNA HOTTIP promotes tumor growth and inhibits cell apoptosis in lung cancer.
In this study, we investigated the role of long non—coding RNA HOTTIP in the tumor growth of lung cancer. Initially, we found that expression of HOTTIP was significantly elevated in 20 cases of lung cancer. HOTTIP was also differentially expressed in a consecutive of lung cancer cell lines. Furthermore, specific shRNA against HOTTIP was employed to deplete expression of HOTTIP in A549 cells and NCI—H446 cells. After successfully depletion of HOTTIP, cell proliferation and colony formation were significantly inhibited in vitro. Tumor growth in vivo was also suppressed after depletion of HOTTIP in a mouse...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Immunohistochemical study of RhoC GTPase in oral squamous cell carcinoma.
This study aimed to evaluate the expression pattern of RhoC in OSCC protein by immunohistochemistry in situ. Immunohistochemical reactions were performed for RhoC by the method of avitina—biotin—peroxidase activity in samples OSCC: well differentiated (BD, n=6), moderately differentiated (MD, n=24) and poorly differentiated (PD, n=13). The morphometry was taken by QuickScore (percentage and intensity of staining) and only intensity staining. There was no statistical difference (p>0.05) through none of the modes of morphometric analysis between BD, MD and PD. And the RhoC staining was not associated w...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Radioprotective effect of chicory seeds against genotoxicity induced by ionizing radiation in human normal lymphocytes.
Authors: Hosseinimehr SJ, Ghaffari-Rad V, Rostamnezhad M, Ghasemi A, Allahverdi Pourfallah T, Shahani S Abstract The search for less—toxic radioprotective agents has led to a growing trend towards natural products. Protective effect of the methanolic extract of chicory seeds (MCS) was investigated against genotoxicity induced by ionizing radiation in human lymphocytes. Human peripheral blood samples were collected and incubated with MCS at different concentrations (10, 50, 100, and 200 μg/mL) for two hours. The whole blood samples were exposed in vitro to X—ray at dose 2.5 Gy. Then, th...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Mutation analysis of the CYP21A2 gene in congenital adrenal hyperplasia.
Mutation analysis of the CYP21A2 gene in congenital adrenal hyperplasia. Cell Mol Biol (Noisy-le-grand). 2015;61(4):51-5 Authors: Forouzanfar K, Seifi M, Hashemi-Gorji F, Karimi V, Estiar MA, Karimoei M, Sakhinia E, Karimipour M Abstract Congenital adrenal hyperplasia (CAH) is an inherited autosomal recessive enzymatic disorder involving the synthesis of adrenal corticosteroids. 21—Hydroxylase deficiency (21—OHD) is the most common form of the disease which is observed in more than 90% of patients with CAH. Early identification of mutations in the genes involved in this di...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

miR-106a* inhibits the proliferation of esophageal carcinoma cells by targeting CDK2-associated Cullin 1 (CACUL1).
In this study, we explored the expression and anti—oncogenic roles of miR—106a* in human EC. The expression of miR—106a* is significantly decreased in EC tissues and EC cell lines. Overexpression of miR—106a* suppressed EC cell proliferation, clonogenicity, G1/S transition, and induced apoptosis in vitro, but inhibition of miR—106a* facilitated cell proliferation, clonogenicity, G1/S transition. Luciferase reporter assay results showed that CDK2—associated Cullin 1 (CACUL1) was a direct target of miR—106a* in EC cells. Moreover, silencing CACUL1 resulted...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Genetics and evolution of plumage color in Crested Ibis: Analysis of the melanocortin-1 receptor (MC1R).
Authors: Yang J, Liu X, Zhang J, Qing B, Lu B Abstract The melanocortin—1—recepter gene (MC1R), an important regulator in melanin synthesis, may cause different plumage color patterns in birds: gain—of— function mutations lead to the synthesis of eumelanin, whereas loss—of—function mutations help to generate pheomelanin synthesis. We had chosen MC1R as a candidate gene for the depigmentation of crested ibis, cloned and sequenced the crested ibis MC1R gene the first time. The crested ibis MC1R sequence, highly conserved with other birds during evolution, ha...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The association of ApE1 -656T>G and 1349T>G polymorphisms with breast cancer susceptibility in northern Iran.
The association of ApE1 -656T>G and 1349T>G polymorphisms with breast cancer susceptibility in northern Iran. Cell Mol Biol (Noisy-le-grand). 2015;61(4):70-4 Authors: Mashayekhi F, Yousefi M, Salehi Z, Saedi HS, Pournourali M Abstract Breast cancer is the most common cancer and one of the main causes of cancer—related death in the world and has become a major public health concern. Many genes have been shown to be involved in the progress of the breast cancer. Human apurinic/apyrimidinic endonuclease 1 (APE1) is a multifunctional protein that has an important role in the base excision r...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Glutamate promotes neural stem cell proliferation by increasing the expression of vascular endothelial growth factor of astrocytes in vitro.
In this study, we investigated the effect of glutamate on the proliferation of rat embryonic neural stem/progenitor cells (NSCs) through regulating the vascular endothelial growth factor (VEGF) expression of astrocytes (ASTs) in vitro, and the cyclin D1 expression of NSCs. The results showed that glutamate promoted the expression and secretion of VEGF of rat astrocytes by activating group I mGluRs. Astrocyte conditioned medium—containing Glu [ACM (30%)] promoted the proliferation of embryonic NSCs compared with normal astrocyte conditioned medium+Glu [N—ACM (30%)+Glu (30 μM)] by increasing cell act...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Molecular cloning and activity analysis of a seed-specific FAD2-1B gene promoter from Glycine max.
In this study, the FAD2—1B gene was determined to be highly expressed in soybean seeds using quantitative real—time PCR(qRT—PCR). To investigate the expression pattern and activity of the FAD2—1B promoter, a 1929 bp 5'—upstream genomic DNA fragment, named PF, was isolated according to the soybean genomic sequence. Sequence analysis revealed the presence of many motifs related to seed—specific promoters in the PF fragment, such as E—box, SEF4, Skn—1 motif, AACACA, AATAAA and so on. Tobacco transgenics carrying the gus reporter gene driv...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Surveillance of gram-positive cocci infections and drug resistance.
In this study, the prevalence of gram—positive cocci isolates and the characteristics of multiple drug resistances in patients were investigated. Antibiotic resistances were determined in the clinical microbiology laboratory with the methodology of the CLSI (2012). The software WHONET5.4 and SPSS13.0 were used for statistical analysis. There were a total of 6211 gram—positive cocci isolates, comprised of 2255 (36.3%) coagulase (—) staphylococci, 1277 (20.6%) staphylococci aureus, 1109 (17.9%) enterococcus faecalis, and 1045 (16.8%) enterococcus faecium. The proportion of Methicillin resistant ...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Expression levels of heat shock protein 60 and glucose-regulated protein 78 in response to trimethylamine-N-oxide treatment in murine macrophage J774A.1 cell line.
This study was therefore designed to evaluate the possible effect(s) of TMAO on the expression of HSP60 and GRP78 at the mRNA and protein levels. Murine macrophage J774A.1 cells were treated with micromolar concentrations of TMAO and 4—phenylbutyric acid (4—PBA), a chemical chaperon, for different time intervals. Tunicamycin was also used as a control for induction of endoplasmic reticulum stress. Tunicamycin greatly increased both mRNA and protein levels of GRP78. Similarly but to a lesser extent compared to tunicamycin, TMAO also increased mRNA and protein levels of GRP78 in a dose and time—...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Comparative phenotypic and genotypic discrimination of methicillin resistant and susceptible Staphylococcus aureus in Egypt.
This study presented the first baseline information on the characterization of MRSA and MSSA in Egypt. PMID: 26429300 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Production and purification of novel thermostable alkaline protease from Anoxybacillus sp. KP1.
In this study, an extracellular novel alkaline protease (EC 3.4.21—24, 99) from a thermophilic and aerobic strain of Anoxybacillus sp. KP1 has been studied. Maximum protease activity was obtained at 50 degC at pH 9.0 after 24 hours of incubation. Among the carbon and nitrogen sources used; the optimum protease production was with soluble starch, maltose, urea and casamino acid. The enzyme was purified by ammonium sulphate precipitation and Sephadex G—75 gel chromatography. Molecular weight of purified enzyme was determined as 106 kDa by SDS—PAGE. Purified protease was stable at 50-60 degC and ...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Targeting microbiota-mitochondria inter-talk: Microbiota control mitochondria metabolism.
Authors: Saint-Georges-Chaumet Y, Attaf D, Pelletier E, Edeas M Abstract Our aim is to highlight the subtle relationship that exists between microbiota and mitochondria. Microbiota targets mitochondria by modulating the Reactive Oxygen Species (ROS) production and the mitochondrial activity through interactions with toxins, proteins or other metabolites released by gut microbiota. The intriguing relationship that exists between mitochondria and microbiota is strengthened by the probable prokaryotic origin of mitochondria. Emerging data implicates a role for ROS, nitric oxide, Short Chain Fatty Acids and hydrogen su...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Micro-RNAs: The new potential biomarkers in cancer diagnosis, prognosis and cancer therapy.
Authors: Mansoori B, Mohammadi A, Shirjang S, Baradaran B Abstract MicroRNAs (miRNAs) are a large class of small noncoding RNAs approximately 22 nucleotides in length. They are the main regulators of gene expression, regulating specific oncogenes, tumor suppressors, cancer stem cells and metastasis. MicroRNAs have become valuable to cancer research in recent years. They appear as a significant biomarker in tumorigenesis. Briefly, the capacities of miRNA to identify between tumor and normal tissue, to distinguish between various subgroups of tumors and to foretell results or responses to therapy have attracted scien...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

SERS and DFT study of p-hydroxybenzoic acid adsorbed on colloidal silver particles.
In this study, normal Raman spectra of p—hydroxybenzoic acid (PHBA) powder and its surface—enhanced Raman scattering (SERS) spectra in silver colloidal solutions were measured under near infrared excitation conditions. In theoretical calculation, two models of PHBA adsorbed on the surfaces of silver nanoparticles were established. The Raman frequencies of these two models using density functional theory (DFT) method were calculated, and compared with the experimental results. It was found that the calculated Raman frequencies were in good agreement with experimental values, which indicates that there ar...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Association of polymorphisms in PRKCI gene and risk of prostate cancer in a sample of Iranian Population.
In conclusion, the results revealed that PRKCI rs546950 variant decreased the risk of PCa in an Iranian population. Further studies with larger sample sizes and different ethnicities are required to confirm our findings. PMID: 26475383 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Citrate and celecoxib induce apoptosis and decrease necrosis in synergistic manner in canine mammary tumor cells.
In conclusion, the present study indicates significant cytotoxic and apoptotic effects of citrate and celecoxib coadministration on CF41—Mg cells, and proposes new strategies for counteracting cancer cells proliferation and overcoming chemo resistance. PMID: 26475384 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Coexistence of plasmid-mediated quinolone resistance determinants and AmpC-Beta-Lactamases in Escherichia coli strains in Egypt.
Authors: Abd El-Aziz NK, Gharib AA Abstract Three kinds of plasmid—mediated quinolone resistance (PMQR) determinants (qnr genes, qepA and aac(6')—Ib—cr) have been discovered and shown to be widely distributed among clinical isolates. To characterize the prevalence of PMQR determinants among AmpC—producing E. coli strains in food—producing animals and animal by—products in Egypt, twenty—nine E. coli strains were tested for their susceptibilities to antimicrobials and screened for PMQR determinants and AmpC Beta lactamases using PCR and pl...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Purification and dose-dependent toxicity study of abrin in swiss albino male mice.
In conclusion, abrin intoxication caused toxic effects and severe damages on studied organs mediated through alteration in biochemical and hematological variables, lipid peroxidation, and degeneration. PMID: 26475386 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Activity of dental pulp cells in semisolid 3D cultures initiated by transforming growth factor-β1 and bone morphogenetic protein 2, 4.
Activity of dental pulp cells in semisolid 3D cultures initiated by transforming growth factor-β1 and bone morphogenetic protein 2, 4. Cell Mol Biol (Noisy-le-grand). 2015;61(5):45-8 Authors: Suzergoz F, Sepet E, Erdem AP, Cinar S, Ikikarakayali G, Erdem MA, Gurol AO Abstract The intention of this study was to investigate the effect of modified 3D culture conditions on dental pulp cells (DPCs). DPCs were isolated from extracted primary molar, premolar, and wisdom teeth. Tooth samples were divided into three groups as control group; plated into methyl cellulose medium without any supplementation, g...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

A peculiar VNTR in the cystathionine β-synthase gene is a risk factor for Down Syndrome.
A peculiar VNTR in the cystathionine β-synthase gene is a risk factor for Down Syndrome. Cell Mol Biol (Noisy-le-grand). 2015;61(5):49-51 Authors: Salemi M, Barone C, Romano C, Salluzzo MG, Giambirtone M, Morale MC, Calogero AE, Grillo L, Bosco P, Romano C Abstract In the present study, we analysed a 31bp variable number of tandem repeats (VNTR) of the cystathionine ß—synthase (CBS) gene in 427 subjects: 127 patients with Down syndrome (DS) and in 60 of their mothers; 172 age—and sex—matched controls and in 68 of their mothers. A significant statistical differen...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Synthesis and molecular characterization of acrylate liquid crystalline resin monomers (ALCRM).
Authors: He XP, Cai W, Guo L, Zhou LZ, Nie MH Abstract A novel biocompatible resin monomer 4—3—(acryloyloxy)—2—hydroxypropoxy) phenyl 4—(3—(acryloyloxy)—2—hydroxypropoxy) benzoate, as an oral restorative — acrylate liquid crystalline resin monomer (ALCRM) was synthesized. The intermediate product and the final product were characterized by differential scanning calorimetry (DSC), polarized optical microscope (POM), and nuclear magnetic resonance (NMR). A resin matrix which has a potential application in dental composites was p...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Protective effect of ellagic acid on oxidative stress and antioxidant status in Cyprinus carpio during malathion exposure.
In conclusion, the findings of this study demonstrated that malathion caused oxidative stress and negative alterations on the antioxidant enzyme activities of the fish. However, this toxic effect was neutralised by the administration of EA. Thus, the present results suggest that simultaneous treatment with EA (100 mg per kg of fish weight) may alleviate malathion—induced oxidative stress. PMID: 26516111 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Guaiasistanol: A new guaiane sesquiterpenoid from Teucrium persicum Boiss.
In this study, a new guaiane type skeleton sesquiterpenoid named guaiasistanol (6α, 10α—epoxy—4α—hydroxyguaiane) was isolated from chloroform part of the Teucrium persicum extract, also chrysothol. Spectroscopic means explained the structures. This compound was evaluated for inhibitory activity against acetylcholinesterase and it showed a moderate activity with 28% inhibition. PMID: 26516112 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Low expression of microRNA-21 contributes to LPS-induced osteoblast cell apoptosis through up-regulation of OAS1.
In this study, we found that LPS triggered apoptosis in osteoblastic hFOB1.19 cells and induced a low expression of the miRNA—21. Furthermore, through the gene microarray technique, OAS1 was screened and later confirmed to be the target gene which was up—regulated in response to the low expression of miRNA—21. Knockdown of OAS1 by specific siRNAs significantly rescued the LPS—induced hFOB1.19 cell apoptosis. Our data suggest that LPS induces low expression of miRNA—21which consequently causes the up—regulation of the downstream gene OAS1 and eventually triggers ap...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

A Short Review: Research progress of bovine stem cells.
Authors: Gao YH, Guan WJ, Ma YH Abstract All bodies rely on stem cells to grow from a single cell into an adult. Stem cells allow our bodies to build new tissue, such as new muscle when we exercise. Domestic livestock stem cells offer a unique opportunity to study developmental biology, serve as a resource to screen for harmful toxins or lifesaving drugs or even regenerative therapies for a number of diseases. This review provides information on bovine stem cells, emphasizing different sources of stem cells and current methods for isolation and culture of pluripotent stem cells from cattle. We also review the appli...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effects of gamma irradiation on chickpea seeds vis-a-vis total seed storage proteins, antioxidant activity and protein profiling.
Authors: Bhagyawant SS, Gupta N, Shrivastava N Abstract The present work describes radiation—induced effects on seed composition vis—à—vis total seed proteins, antioxidant levels and protein profiling employing two dimensional gel electrophoresis (2D—GE) in kabuli and desi chickpea varities. Seeds were exposed to the radiation doses of 1,2,3,4 and 5 kGy. The total protein concentrations decreased and antioxidant levels were increased with increasing dose compared to control seed samples. Radiation induced effects were dose dependent to these seed parameters while it sh...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

DNA polymorphisms in chickpea accessions as revealed by PCR-based markers.
Authors: Yadav P, Koul KK, Shrivastava N, Mendaki MJ, Bhagyawant SS Abstract Chickpea is a food legume which is alleged to be a preferred source of protein next only to milk. Germplasm of cultivated chickpea available is deficient in desired genetic variation. Genetic manipulations therefore, necessitate the genetic exploitation of its related annual and wild species. 42 RAPD and 41 ISSR markers were employed to ascertain polymorphism across 20 genotypes which were collected from 10 different geographical areas of the world. RAPD marker detected 51% genetic polymorphisms while ISSR marker detected 54 %. With an ave...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

ASPP and iASPP: Implication in cancer development and progression.
Authors: Li Y, Ahmad A, Sarkar FH Abstract The well—known guardian of genome, p53 plays critical roles in the induction of apoptosis typically upon DNA damage whereas mutant p53 containing cells are unable to undergo apoptosis which leads to aggressive tumor growth and drug resistance. Moreover, another molecule regulating wild—type p53 function is ASPP (apoptosis stimulating proteins of p53) family. ASPP family consists of ASPP1 and ASPP2, and functions as tumor suppressors whereas the inhibitor of ASPP (iASPP) functions as oncogene. By binding to apoptosis regulating proteins such as p53, p63,...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Association of NOTCH with different microRNAs in head and neck cancer.
Authors: Masood N, Qureshi MZ, Yasmin A Abstract Head and neck cancer is the sixth most common cancer worldwide with an alarming increase in Asian countries. Overwhelmingly increasing cell culture and preclinical studies are identifying wide ranging mechanisms which are instrumental in disease development, progression and resistance against different therapeutics. The scientists are unable to differentiate whether expressional mutation is a cause or a consequence of some other alterations occurring in the body. We partition this review into how NOTCH1 and p16 contribute in cancer development and how microRNAs quant...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The role of microRNAs in the resistance to colorectal cancer treatments.
Authors: Amirkhah R, Farazmand A, Irfan-Maqsood M, Wolkenhauer O, Wolkenhauer O, Schmitz U Abstract Colorectal cancer (CRC) is one of the leading cancer—related causes of death in the world. Several approaches such as surgery, chemotherapy, radiotherapy, targeted therapy, or combinations thereof have been used to treat CRC patients. However, the fact that many patients develop a drug resistance during the course of the treatment is a major obstacle. Understanding the mechanisms underlying resistance is critical in order to develop more effective targeted treatments. Recently, several studies have reported...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The Notch signaling pathway in esophageal adenocarcinoma.
Authors: Wang Z, Chen J, Capobianco AJ Abstract The Notch signaling pathway plays a critical role in embryonic development, self—renewal of stem cells, and carcinogenesis. Aberrant Notch signaling has been linked to a wide variety of cancers, and can either suppress or promote tumors depending on the cell type and the context. Increasingly it is being realized that Notch signaling not only involves in the pathogenesis and development of esophageal adenocarcinoma (EAC), it also promotes the growth of EAC cells and also involved in the maintenance of EAC cancer stem cells. The efficacy of gamma—se...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

TRAIL and targeting cancer cells: between promises and obstacles.
Authors: Limami Y, Pinon A, Riaz A, Simon A Abstract Targeting cancer cells is one of the challenges of current treatment strategies. TRAIL represents a promising therapeutic approach and over the past decades there was an increased interest in targeting TRAIL signaling to treat cancer. Indeed, TRAIL can specifically target cancer cells and exhibits very low cytotoxicity towards normal cells. However, rapidly accumulating experimental evidence has started to shed light on multiple factors which induce resistance against TRAIL in cancer cells. This resistance consists of various mechanisms including downregulation o...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Cancer stem cells in head and neck cancer: A Mini Review.
Authors: Silva Galbiatti-Dias AL, Pavarino ÉC, Kawasaki-Oyama RS, Maniglia JV, Maniglia EJ, Goloni Bertollo EM Abstract Head and neck cancer (HNC) is a multifaceted and genomically complex disease and rapidly emerging preclinical and clinical studies have provided a broader landscape of signaling. It is being realized that intra—tumor heterogeneity, genetic and epigenetic mutations considerably challenge wide ranging therapeutics and patients frequently develop locoregional recurrences, second primary tumours and distant metastases. Using high—throughput technologies, it has been revealed...
Source: Cellular and Molecular Biology - December 2, 2015 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research