The effects of dietary organic selenium on growth, body composition and hematological parameters of common Carp (Cyprinus carpio) reared in recirculating aquaculture system
Cell Mol Biol (Noisy-le-grand). 2024 Jan 31;70(1):87-93. doi: 10.14715/cmb/2024.70.1.12.ABSTRACTA feeding trial was conducted to examine the effects of different levels of dietary organic selenium (Se) on the growth, body composition, and hematological indices of common carp. Se element was supplemented to the basal diet at the rates of 0.0, 0.5, 1 and 2 mgkg-1. Fish were randomly stocked at a rate of 25 fish into 500L tanks in the Recirculating Aquaculture System (RAS) system for 10 weeks. Weight gain, final body weight, and specific growth rate were significantly higher (p<0.05) in fish fed 0.5 mgkg-1 followed by 1 an...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Ibrahim Abdulrahman Mustafa Samad Sofy Omar Source Type: research
Enhancing the efficiency and functionality of xylanase from Bacillus sp. RTS11: Optimization, purification, characterization, and prospects in kraft pulp bleaching
Cell Mol Biol (Noisy-le-grand). 2024 Jan 31;70(1):67-79. doi: 10.14715/cmb/2024.70.1.10.ABSTRACTBacillus sp. RTS11, a xylanolytic strain, was isolated from the Algerian desert rocks. Genetic analysis revealed a remarkable 98.69% similarity to Bacillus pumilus. We harnessed optimization techniques, including Plackett-Burman screening and Box-Behnken optimization design, to amplify xylanase production and activity. The outcome of these efforts was an optimized medium that yielded an impressive xylanase production titer of 448.89 U, a threefold increase compared to the non-optimized medium (146 U). The Purification of xylanas...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Sara Sahnoun Bilal Yahiaoui Aicha Benlounissi Abdenacer Mouffok Barbara Ernst Manawwer Alam Karim Houali Yacine Benguerba Source Type: research
Repair effect of neurotrophic factor III (NT-3) on rats with spinal injury model and its mechanism
Cell Mol Biol (Noisy-le-grand). 2024 Jan 31;70(1):56-61. doi: 10.14715/cmb/2024.70.1.8.ABSTRACTThe present study aimed to study the repair effect of neurotrophic factor III (NT-3) on spinal injury model rats and its mechanism. Wistar rats with spinal injury were established by accelerated compression stroke after the operation and divided into control group, model group, and NT-3 intervention group. The motor function of rats in each group was evaluated at different postoperative time points (3, 7, 14 d). HE staining was used to detect the changes in tissue structure and morphology of the injured spinal column in each grou...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Dexin Zou Huimin Wang Sibin Hao Feng Chen Source Type: research
The role of silymarin and MAPK pathway in the regulation of proliferation and invasion of non-small cell lung cancer cells
Cell Mol Biol (Noisy-le-grand). 2024 Jan 31;70(1):62-66. doi: 10.14715/cmb/2024.70.1.9.ABSTRACTWe aimed to explore the role of silymarin and mitogen-activated protein kinase (MAPK) pathway in the regulation of proliferation and invasion of non-small cell lung cancer cells. Non-small cell lung cancer cells were cultured and divided into groups and treated with drugs, and A blank control group was set up. The concentration of silymarin in the experimental group was 10 mg/L, 20 mg/L and 40 mg/L, respectively, which were recorded as groups A, B and C, and three repeated experiments were performed in each group. Absorbance (A v...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Jing Zhang Kaige Zhang Jing Li Jia Zhang Xiaohai Cui Source Type: research
High expression of VTA1 is an adverse prognostic factor in lung adenocarcinoma
Cell Mol Biol (Noisy-le-grand). 2024 Jan 31;70(1):46-55. doi: 10.14715/cmb/2024.70.1.7.ABSTRACTLung adenocarcinoma (LUAD) is a common pathological type of non-small cell lung cancer; identifying preferable biomarkers has become one of the current challenges. Given that VTA1 has been reported associated with tumor progression in various human solid cancers but rarely reported in LUAD, herein, RNA sequencing data from TCGA and GTEx were obtained for analysis of VTA1 expression and differentially expressed gene (DEG). Furthermore, functional enrichment analysis of VTA1-related DEGs was performed by GO/KEGG, GSEA, immune cell ...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Xingang Sun Ruixin Wu Xinjun Guan Changsheng Dong Dongze Qiu Guojie Xia Shouhan Feng Jinlong Duan Lei Zhang Source Type: research
MiR-146a alleviates acute lung injury via inhibiting Notch 1 signaling pathway targeting macrophage
Cell Mol Biol (Noisy-le-grand). 2024 Jan 31;70(1):34-39. doi: 10.14715/cmb/2024.70.1.5.ABSTRACTAcute lung injury (ALI) is associated with the leukocyte infiltration and inflammation. Previous studies have shown that miR-146a is a valid regulator of the macrophage polarization in vitro inflammatory model. However, it is unclear whether miR-146a plays a protective role in ALI via modulating macrophage inflammation. To explore the potential therapeutic effect mechanism of miR-146a on ALI. We analyzed the expression of miR-146a in acute injured lung tissues and differentiated macrophage. Lipopolysaccharide (LPS) and interleuki...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Qiumin Zhao Yan Wang Jing Zou Rong Kuang Shiyan Ji Source Type: research
Comparative study on the expression of Pax3, Rad51 and VEGF-C in esophageal gastric junction adenocarcinoma and distal gastric adenocarcinoma
Cell Mol Biol (Noisy-le-grand). 2024 Jan 31;70(1):40-45. doi: 10.14715/cmb/2024.70.1.6.ABSTRACTThe purpose of this study was to explore the differential expression of Pax3, Rad51 and VEGF-C in esophageal gastric junction adenocarcinoma and distal gastric adenocarcinoma and their relationship with cancer occurrence and development. 57 patients with gastric cancer were included and divided into esophageal gastric junction adenocarcinoma group (n=28) and distal gastric adenocarcinoma group (n=29). The positive expressions of Pax3, Rad51 and VEGF-C in the control group were lower than those in the esophageal gastric junction a...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Qiang Pan Xiangyang Liu Chen Xu Chengyuan Yu Source Type: research
MiR-22 inhibits myocardial fibrosis in rats with myocardial infarction by targeting PTEN/Akt/mTOR signaling pathway
This study aimed to evaluate the effects of miR-22 on myocardial fibrosis in rats with myocardial infarction (MI) and to further explore the possible underlying mechanism. A total of 80 rats were randomly divided into Sham group, miR-22 overexpression group, MI group or MI + miR-22 overexpression group. Reverse transcription-polymerase chain reaction (RT-PCR) results showed that compared with Sham group, miR-22 expression level in myocardial tissues of rats decreased significantly in MI group. Overexpression of miR-22 could remarkably relieve cardiac insufficiency in MI rats, increase EF% and FS%, and reduce collagen depos...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Bolin Li Chunna Liang Yuanjun Lv Yongjin Tan Weiyan Chen Source Type: research
Cerium oxide nanoparticles impact on sepsis-induced cerebral injury: Deciphering miRNA / NF- κB/TLR signalling pathway
Cell Mol Biol (Noisy-le-grand). 2024 Jan 31;70(1):19-27. doi: 10.14715/cmb/2024.70.1.3.ABSTRACTSepsis is regarded as an inflammatory syndrome that consists of complex biochemical and pathophysiological dysregulation, brought on by endogenous factors in response to systemic infection. Sepsis can cause short- and long-term cerebral injury. Cerium oxide nanoparticles (CeO2 NPs) have been reported to possess both anti-inflammatory and antioxidative properties. The current study investigated the potential role of cerium oxide nanoparticles in the management of sepsis-induced brain injury. To achieve this target, forty male albi...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Wesam H Abdulaal Nawal Helmi Amal Hamza Neveen A Salem Source Type: research
SARS-CoV-2 receptor a distinct genetic profile specific to the Iraqi Kurdish population
Cell Mol Biol (Noisy-le-grand). 2024 Jan 31;70(1):12-18. doi: 10.14715/cmb/2024.70.1.2.ABSTRACTSARS-CoV-2, the virus responsible for COVID-19, enters host cells by binding its spike protein's receptor-binding domain (RBD) to the human angiotensin-converting enzyme 2 (ACE2) receptor's peptidase domain (PD). This interaction plays a crucial role in the virus's ability to invade host cells and establish infection. Numerous studies have identified specific residues crucial for their binding interaction. Our objective was to determine whether natural variations in the ACE2 receptor could impact its affinity for the S-protein RB...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Suhad Asaad Mustafa Source Type: research
Autophagy related genes mediated mitophagy in yeast, mammals and higher plants
Cell Mol Biol (Noisy-le-grand). 2024 Jan 31;70(1):1-11. doi: 10.14715/cmb/2024.70.1.1.ABSTRACTAutophagy is classified into macro-autophagy and micro-autophagy. Two major types of autophagy in the complex eukaryotic organism are microautophagy and macroautophagy. During microautophagy, cytoplasmic components that need to be degraded are taken up by lysosomes in animals and by vacuole in yeast and plants via the invagination of tonoplast. While macroautophagy is initiated after the formation of a cup-shaped membrane structure, a phagophore develops at cargo that grows in size and is sealed by double-membrane vesicles to form...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Muhammad Riaz Razia Sultana Javed Ahmad Azhar Mehmood Saira Sattar Muhammad Hammad Tanveer Muhammad Zulkiffal Muhammad Sarwar Source Type: research
Cytoplasmic expression of UTP23 promotes colorectal cancer progression
Cell Mol Biol (Noisy-le-grand). 2024 Jan 31;70(1):239-245. doi: 10.14715/cmb/2024.70.1.33.ABSTRACTUTP23 (UTP23 small subunit processome component) plays a pivotal role in the intricate processing and maturation of the small subunit of ribosomes within the nucleolus. In cases of nucleolar stress, such as those observed in certain tumor cells, the aberrant nucleolar organization and structure can lead to the translocation of nucleolar proteins into the nucleus or cytoplasm, consequently impacting the physiological processes of the tumor cells through non-ribosome-related functions. Our investigation revealed altered localiza...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Fang E Lili Qian Jinlong Tang Zhaowei Tong Source Type: research
Ginsenoside Rg3 exhibits anti-catabolic and anti-apoptotic effects in IL-1 β treated human disc nucleus pulposus cells and in a rat model of disc degeneration by inactivating the MAPK pathway
This study aimed to investigate the regulatory functions of ginsenoside Rg3 in IDD. We established the IDD cell model via inducing NPCs with IL-1β. The rat model of IDD was established by fibrous ring puncture method. Cell apoptotic capability was assessed through TUNEL assay. The levels of catabolic proteases MMPs and ADAMTSs were tested by western blot and RT-qPCR. IL-1β induction notably promoted the apoptosis of NPCs, while ginsenoside Rg3 treatment reversed the promoting function of IL-1β. Furthermore, we found that MMP2, MMP3, Adamts4, and Adamts5 levels were increased in IL-1β-induced NPCs, while ginsenoside Rg3...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Jie Chen Bin Zhang Liwei Wu Jiabin Xu Source Type: research
Inhibition of RXRA-mediated PLA2G2A improves delirium in COPD mice by regulating endoplasmic reticulum stress pathway and inhibiting cell apoptosis
Cell Mol Biol (Noisy-le-grand). 2024 Jan 31;70(1):226-232. doi: 10.14715/cmb/2024.70.1.31.ABSTRACTDelirium is a common psychiatric complication of chronic obstructive pulmonary disease (COPD). The relief of delirium is considered one of the beneficial ways to treat COPD. However, there are currently no specific drugs that alleviate delirium in COPD patients. Our research aimed to elucidate the specific mechanisms underlying delirium in COPD mice, while also seeking more effective therapeutic targets. In our study, bioinformatics analysis and qRT PCR were used to identify key factors in the development of delirium in COPD a...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Teng Zhang Guodong Wang Source Type: research
Dihydrotestosterone reduces neuroinflammation in spinal cord injury through NF- κB and MAPK pathway
Cell Mol Biol (Noisy-le-grand). 2024 Jan 31;70(1):213-218. doi: 10.14715/cmb/2024.70.1.29.ABSTRACTNeuroinflammation induced by microglia following spinal cord injury (SCI) leads to secondary neurologic injury. Androgens including testosterone and dihydrotestosterone (DHT) show as endogenous neuroprotective factors against multiple neurologic diseases, while their therapeutic role in SCI-induced neuroinflammation and underlying mechanism remains elusive. In the study, we aimed to investigate the role of DHT against microglia-induced neuroinflammation in SCI and evaluate its protective treatment. BV2 cells were activated by ...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Jiarui Wei Tao Li Shengyuan Lin Bin Zhang Xing Li Source Type: research