Genetic diversity of multidrug resistant Staphylococcus aureus isolated from clinical and non clinical samples in Egypt.
Authors: Bendary MM, Solyman SM, Azab MM, Mahmoud NF, Hanora AM Abstract In recent years, the increasing incidence of diseases caused by Staphylococcus aureus (S. aureus) has been noted in the university hospitals of El-Sharkia and Assuit governorates - Egypt. Therefore, we studied the genetic relatedness of multidrug resistant S. aureus isolates from different sources in the above mentioned governorates. One hundred and fifty six S. aureus isolates were divided into 5 different groups, 1 non clinical isolates from different food products and 4 different clinical isolates of human and animal sources in the 2 differ...
Source: Cellular and Molecular Biology - September 10, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Three-dimensional stress In vitro promotes the proliferation and differentiation of periodontal ligament stem cells implanted by bioactive glass.
Authors: Wang T, Li G, Chen J, Lin Z, Qin H, Ji J Abstract To analyze the biological and mechanical microenvironment on the directional differentiation of periodontal ligament stem cells (PDLSCs) In vitro. PDLSCs were cultured in three-dimensional stress system In vitro for 1, 2 and 3 weeks. Methods like immunohistochemistry and flow cytometry were adopted and the proliferation and differentiation situation of PDLSCs were determined. Bioactive glass (BAG) of 0%, 10%, 20%, 30% and 40% was implanted into PDLSCs with or without three-dimensional stress for 3 weeks, respectively. The proliferation and differentiation s...
Source: Cellular and Molecular Biology - September 10, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Comparative characteristics of mesenchymal stem cells derived from reamer-irrigator-aspirator, iliac crest bone marrow, and adipose tissue.
In this study we compared MSCs derived from three different sources (iliac crest bone marrow (ICBM), adipose tissue (AT), and (RIA)) regarding the morphology, the success rate of isolating MSCs, colony frequency, expansion potential, osteogenic and chondrogenic differentiation capacity. MSCs were isolated from three different sources and flow cytometric analyses were performed for cell characterization. Colony-forming unit-fibroblast (CFU-F) assay and population doubling time (PDT) were evaluated for MSCs derived from three different sources and differentiation potential of RIA, ICBM-, and AT-MSCs were determined by staini...
Source: Cellular and Molecular Biology - September 10, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Mitosis-specific phosphorylation of PML at T409 regulates spindle checkpoint.
Authors: Jin J, Liu J Abstract During mitosis, Promyelocytic leukemia nuclear bodies (PML NBs) change dramatically in morphology and composition, but little is known about function of PML in mitosis. Here, we show that PML is phosphorylated at T409 (PML p409) in a mitosis-specific manner. More importantly, PML p409 contributes to maintain the duration of pro-metaphase and regulates spindle checkpoint. Deficient PML p409 caused a shortening of pro-metaphase and challenged the nocodazole-triggered mitotic arrest. T409A mutation led to a higher frequency of misaligned chromosomes on metaphase plate, and subsequently d...
Source: Cellular and Molecular Biology - September 10, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The antiproliferative effect of Origanum majorana on human hepatocarcinoma cell line: suppression of NF- κB.
This study aimed to investigate the antiproliferative effect of aqueous and ethanol extracts of Origanum majorana leaf on human hepatocellular carcinoma (HepG2) cell line through incubation of various concentrations of origanum majorana extracts with HepG2 and at different time intervals. The effects of aqueous and ethanol extracts of O. majorana L. on HepG2 cell viability, nuclear factor kappa B (NF-kB) gene expression were examined. The results of the cell viability assays showed that aqueous and ethanol extracts exhibited a highly significant inhibitory effect on HepG2 cell proliferation which was evidenced by a reducti...
Source: Cellular and Molecular Biology - September 10, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Spirulina exhibits hepatoprotective effects against lead induced oxidative  injury in newborn rats.
Spirulina exhibits hepatoprotective effects against lead induced oxidative injury in newborn rats. Cell Mol Biol (Noisy-le-grand). 2016;62(10):85-3 Authors: Gargouri M, Ben Saad H, Ben Amara I, Magné C, El Feki A Abstract Lead is a toxic metal that induces a wide range of biochemical and physiological effects. The present investigation was designed at evaluating the toxic effects of a prenatal exposure to lead of mothers on hepatic tissue of newborn rats, and potent protective effects of spirulina. Female rats were randomly divided into 4 groups which were given a normal diet (control),a di...
Source: Cellular and Molecular Biology - September 10, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Herbal Antibiotics: Moving back into the mainstream as an alternative for "Superbugs".
Herbal Antibiotics: Moving back into the mainstream as an alternative for "Superbugs". Cell Mol Biol (Noisy-le-grand). 2016;62(9):1-2 Authors: Sharifi-Rad J Abstract Editorial. PMID: 27585254 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - September 3, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Factors contributes to spontaneous abortion caused by Listeria monocytogenes, in Tehran, Iran, 2015.
Authors: Pourkaveh B, Ahmadi M, Eslami G, Gachkar L Abstract Spontaneous abortion is the loss of a fetus before the 20th week of pregnancy, when occurring naturally without any surgical or pharmaceutical intervention. On the other hand, Listeria monocytogenes, as one of the foodborne pathogens, is a causative agent of listeriosis. The transfer of L. monocytogenes in pregnant women occurs as self-limited flu-like symptoms which may result in abortion, stillbirth or premature birth of infected infants. The purpose of this study was the identification of Listeria monocytogenes risk factors in women with spontaneous ab...
Source: Cellular and Molecular Biology - September 3, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Potential effect of Olea europea leaves, Sonchus oleraceus leaves and Mangifera indica peel extracts on aromatase activity in human placental microsomes and CYP19A1 expression in MCF-7 cell line: Comparative study.
In this study, different plant extracts from Olea europaea leaves (OLE), Sonchus oleraceus L. (SOE) and Mangifera indica peels (MPE) were prepared to identify phytoconstituents and measure antioxidant capacities. The effects of these three extracts on aromatase activity in human placental microsomes were evaluated. Additionally, the effects of these extracts on tissue-specific promoter expression of CYP19A1 gene in cell culture model (MCF-7) were assessed using qRT-PCR. Results showed a concentration-dependent decrease in aromatase activity after treatment with OLE and MPE, whereas, SOE showed a biphasic effect. The differ...
Source: Cellular and Molecular Biology - September 3, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Tordylium persicum Boiss. & Hausskn extract: A possible alternative for treatment of pediatric infectious diseases.
Tordylium persicum Boiss. & Hausskn extract: A possible alternative for treatment of pediatric infectious diseases. Cell Mol Biol (Noisy-le-grand). 2016;62(9):20-6 Authors: Sharifi-Rad J, Fallah F, Setzer WN, Entezari Heravi R, Sharifi-Rad M Abstract Antimicrobial herbal compounds are one of the important medical resources, and in order to help alleviate the spread of the pediatric infectious diseases, identification of additional bioactive phytochemicals and herbal extracts will be practical in treating illnesses. In the present work, antimicrobial activities various extracts of Tordylium persicum...
Source: Cellular and Molecular Biology - September 3, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Potential of Ocimum basilicum L. and Salvia officinalis L. essential oils against biofilms of P. aeruginosa clinical isolates.
Authors: Stojanović-Radić Z, Pejcić M, Stojanović N, Sharifi-Rad J, Stanković N Abstract Biofilms are complex communities of microorganisms, responsible for more than 60% of the chronic human infections and they represent one of the leading concerns in medicine. Pseudomonas aeruginosa is human pathogenic bacteria which causes numerous diseases and is known for its ability to produce biofilm. Ocimum basilicum L. (basil) and Salvia officinalis L. (sage) are widely used plants in traditional medicine for the treatment of different conditions. Therefore, the aim of this study was to investigate the potential of ba...
Source: Cellular and Molecular Biology - September 3, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Association of P53 codon 72 polymorphism and lung cancer in an ethnic Iranian population.
Authors: Eydian Z, Asna'ashari AM, Behravan J, Sharifi-Rad J, Entezari Heravi R Abstract Lung cancer is one of the most common causes of cancer death worldwide. Molecular genetic studies indicated that activation of dominant oncogenes or inactivation of tumor suppressor genes and the presence of polymorphism in these genes correlated with prevalence of new lung cancers. P53 as a tumor suppressor gene located at 17p13 chromosome and it is one of the most well-known mutant genes in all cancer types. Mutation in P53 can disturb the transcriptional function and suppression of cell cycle control and increase in cell div...
Source: Cellular and Molecular Biology - September 3, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Anti-methicillin-resistant Staphylococcus aureus (MRSA) activity of Rubiaceae, Fabaceae and Poaceae plants: A search for new sources of useful alternative antibacterials against MRSA infections.
In this study, we evaluated the effects of the extracts of the leaves of species from the Rubiaceae (Galium aparine L. and Asperula arvensis L.), Fabaceae (Lathyrus aphaca L. and Vicia narbonensis L.) and Poaceae (Digitaria sanguinalis (L.) Scop. and Hordeum murinum L.) plant families on a wide and extensive panel of isolated methicillin-resistant Staphylococcus aureus strains (MRSA). The effects of the methanolic leaf extracts of Rubiaceae, Fabaceae and Poaceae plants on MRSA were evaluated by the disc diffusion assay and the broth dilution method. Among a total of 177 S. aureus isolates, 92 (51.97%) were found to be meth...
Source: Cellular and Molecular Biology - September 3, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Green synthesis of silver nanoparticles using Salvadora persica L. and its antibacterial activity.
In this study, AgNPs was synthesized using aqueous extract of Salvadora persica bark. The synthesized AgNPs were characterized by UV-Vis spectroscopy, Powder X-ray diffraction (PXRD) and Transmission electron microscopy (TEM) methods. The optimal synthesis condition to prepare nanoparticles was determined as silver nitrate 3 mM, 5 ml of aqueous extract in the room temperature for 1 h. The TEM image of AgNPs showed the formation of spherical, non-uniform nanoparticles of mean size of 50 nm. The antibacterial activity of synthesized AgNPs was evaluated using disk diffusion, minimum inhibitory concentration (MIC) and minimum ...
Source: Cellular and Molecular Biology - September 3, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

A comparison between PLGA-PEG and NIPAAm-MAA nanocarriers in curcumin delivery for hTERT silencing in lung cancer cell line.
Authors: Roointan A, Sharifi-Rad M, Badrzadeh F, Sharifi-Rad J Abstract Lung cancer is the most common cancer among men. Since the main reason of cancer cells immortality is telomerase activity, targeting of such enzyme can be a promising approach in cancer therapy. Curcumin is a safe and efficient anticancer agent in this context, but its applications in cancer therapy are limited because of its hydrophobic structure and low solubility in water. Today, using nanocarriers for delivery of such anticancer agents is a well performed method. Here, we developed and compared the efficiency of two nanocarriers (PLGA-PEG a...
Source: Cellular and Molecular Biology - September 3, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Plants of the genus Allium as antibacterial agents: From tradition to pharmacy.
Authors: Sharifi-Rad J, Mnayer D, Tabanelli G, Stojanović-Radić ZZ, Sharifi-Rad M, Yousaf Z, Vallone L, Setzer WN, Iriti M Abstract Plants belonging to the genus Allium are widely cultivated and used all over the world as food and medicinal plants. Since ancient times, these plants, particularly garlic (Allium sativum L.) and onion (Allium cepa L.), have represented important components of typical recipes and traditional healing systems. Not the least of which, their use as food biopreservatives is well documented, due to the relevant antibacterial activity of their extracts and essential oils. In addition to gar...
Source: Cellular and Molecular Biology - September 3, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Methicillin-Resistant Staphylococcus Aureus infection exacerbates NSCLC cell metastasis by up-regulating TLR4/MyD88 pathway.
In conclusion, MRSA infection can enhance NSCLC cell metastasis by up-regulating TLR4/MyD88 signaling. PMID: 27545207 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - August 24, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Helicobacter pylori infection and iron deficiency in patients with coronary artery disease.
Authors: Fallah S, Ahmadi R, Moradi N, Fadaei R, Sezavar SH, Seifi M Abstract The aim of this study was to investigate whether impact of the seropositivity to Helicobacter pylori (H pylori) infection on ferritin and iron levels is an independent risk factor for atherosclerosis in patients with cardiovascular disease. The anti H pylori IgG, IgA levels, serum ferritin and iron concentration of 86 patients with cardiovascular disease and 64 participants free of cardiovascular disease as control subjects were determined by ELISA assay. The results of present study showed that seropositivity to H pylori IgG and IgA leve...
Source: Cellular and Molecular Biology - August 24, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Strain differentiation of Mycobacterium tuberculosis for epidemiology in northwest of Iran.
In this study, 194 positive cultures of M. tuberculosis in North West Iran were evaluated by the MIRU-VNTR method. MIRU-12 differentiated the 194 isolates into 138 different patterns, comprising 30 clusters and 108 unique patterns (HGDI=0.9930). The largest cluster contained twelve isolates. The results showed that the majority of TB cases in North West Iran are due to reactivation and the 12-MIRU typing method can be used as a first-line method for strain differentiation of M. tuberculosis. PMID: 27545209 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - August 24, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Preliminary characterization of human skin microbiome in healthy Egyptian individuals.
This study presents a new insight for subsequent analyses of human microbiome in Egypt. PMID: 27545210 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - August 24, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Hemodynamic shear stress regulates the transcriptional expression of heparan sulfate proteoglycans in human umbilical vein endothelial cell.
Authors: Liu JX, Yan ZP, Zhang YY, Wu J, Liu XH, Zeng Y Abstract We have previously demonstrated the adaptive remodeling of endothelial glycocalyx under shear stress. However, the underlying mechanism in glycocalyx remodeling, especially the expression of the components heparan sulfate proteoglycans (HSPGs) under shear stress was not completely known. In the present study, we investigated the expression of those HSPGs (syndecan family and glypican-1) in human umbilical vein endothelial cells (HUVECs) responded to the distinct magnitude of shear stress, and performed a systematic and comprehensive analyze on the rel...
Source: Cellular and Molecular Biology - August 24, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Can rs3767140 SNP of the perlecan (HSPG2) gene affect the diabetes mellitus through the dyslipidemia?
Authors: Kurnaz-Gömleksiz Ö, Tokat B, Aslan EI, Yanar F, Ermiş-Karaali Z, Öztürk O, Yilmaz-Aydoğan H Abstract Perlecan (HSPG2) play an important role in the lipoprotein metabolisms. The G allele of the HSPG2-rs3767140 may affect the binding of heparan sulfate (HS) chains and hence cause loss of HS from the basement membrane. HSPG2-rs3767140 was studied in 60 T2DM patients and 109 healthy controls. In diabetic patients HSPG2-rs3767140 T variant allele carriers (TT+GT) have decreased fasting plasma glucose (FPG) and serum LDL-C levels (p=0.071 and p=0.060, respectively) versus GG genotype carrie...
Source: Cellular and Molecular Biology - August 24, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Irisin immunostaining characteristics of breast and ovarian cancer cells.
Authors: Kuloglu T, Celik O, Aydin S, Hanifi Ozercan I, Acet M, Aydin Y, Artas G, Turk A, Yardim M, Ozan G, Hanifi Yalcin M, Kocaman N Abstract To determine expression pattern of irisin in tissues obtained from human ovarian cancer, breast cancer, and cervix cancer. Tissue samples obtained from subjects with breast cancer, ovarian cancer cervix cancer, simple endometrial hyperplasia, complex atypical endometrial hyperplasia. At least five sections from each subject were immunohistochemically stained with irisin antibody, and H-score method was used to evaluate irisin intensity. Tissues obtained from healthy breast ...
Source: Cellular and Molecular Biology - August 24, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Cloning and expression of recombinant human platelet-derived growth factor-BB in Pichia Pink.
Authors: Babavalian H, Latifi AM, Shokrgozar MA, Bonakdar S, Tebyanian H, Shakeri F Abstract The PDGF-BB plays a key role in several pathogenesis diseases and it is believed to be an important mediator for wound healing. The recombinant human PDGF-BB is safe and effective to stimulate the healing of chronic, full thickness and lower extremity diabetic neurotrophic ulcers. In the present study, we attempted to produce a PDGF-BB growth factor and also, evaluate its functionality in cell proliferation in yeast host Pichia pink. Pichia pink yeast was used as a host for evaluation of the rhPDGF-BB expression. The coding...
Source: Cellular and Molecular Biology - August 24, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

PPAR-gamma in overcoming kinase resistance in chronic myeloid leukemia.
Authors: Yousefi B, Shafiei-Irannejad V, Azimi A, Samadi N, Zarghami N Abstract Peroxisome proliferator-activated receptor gamma (PPARγ) plays key roles in regulating cellular differentiation, proliferation and apoptosis pathways. As such, they are considered promising targets for anticancer drug development, especially for breast cancer, multiple myeloma and hematologic malignancies. Chronic myeloid leukemia (CML) is a myeloproliferative disorder arising from an oncogenic Bcr-Abl tyrosine kinase. Inhibitors of this oncogene by small molecules such as imatinib are effective only in 75% of the patient's popula...
Source: Cellular and Molecular Biology - August 24, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Lanthanum chloride enhances cisplatin-induced apoptosis in ovarian cancer cells.
In this study, we investigated the influence of LaCl3 on cisplatin-induced apoptosis in the ovarian cell line (COC1). Cell-based assays and analysis of cellular ultra structure were used. Result showed that LaCl3 enhanced cisplatin-induced apoptosis in COC1. Furthermore, the application of Cisplatin also altered the expression level of tumor related proteins, such as ERCC1, Ki67, and CDK6. Overall, we determined the inhibitory effect of LaCl3 on the COC1 growth when treated with Cisplatin, which may serve as a basis for the clinical application of LaCl3 in the treatment of ovarian cancer. PMID: 27453264 [PubMed - in p...
Source: Cellular and Molecular Biology - July 29, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Synthesis, SAR and biological evaluation of a novel series of 1-(2-chloroethyl)-1-nitroso-3-(2-(3-oxobenzoelenazol-2(3H)-yl)ethyl) urea: Organoselenium compounds for cancer therapy.
Authors: Ye S, Zheng X, Hu T, Zeng H Abstract Thioredoxin reductase 1 (TrxR1) is an important potential anticancer drug target and closely related to both carcinogenesis and cancer progression. Ethaselen (BBSKE), a novel organoselenium compound inhibiting TrxR1 with selective antitumor effect, while its symmetrical structure results in poor solubility. Carmustine (BCNU), a DNA cross-link agent and also a deactivator of TrxR, is with high toxicity and low selectivity which limit its clinical application to some extents. Herein, a novel compound, 1-(2-chloroethyl)-1-nitroso-3-(2-(3-oxobenzoel...
Source: Cellular and Molecular Biology - July 29, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effect of FUT3 gene silencing with miRNA on proliferation, invasion and migration abilities of human KATO-III gastric cancer cell line.
This study investigated the effects of FUT3 gene expression inhibition with miRNA on the proliferation, invasion and migration abilities of KATO-III cells. KATO-III cells were transfected with plasmid pcDNA™6.2-GW/EmGFP-FUT3-miR(FUT3-miRNA) and negative control plasmid in mediation of liposome, respectively, using untransfected cells as blank controls. Forty-eight hours after transfection, FUT3 mRNA levels were tested by RT-PCR. Levels of sLeA proteins were assayed by Western blot. The effects of FUT3-miRNA on the proliferation, invasion and migration of KATO-III cells were determined by CCK8 testing and Transwell as...
Source: Cellular and Molecular Biology - July 29, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

A new neurotoxicity model composed of a neurovascular unit in vitro.
In conclusion, the two models replicated the blood-brain barrier (BBB) more accurately than the control, so it has potential usefulness in further scientific and clinical drug research. Furthermore, NVU model could be used to screen neurotoxicity chemicals due to its NVU properties. PMID: 27453267 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - July 29, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Toxicity of Hoechst 33342: implication in side population analysis.
Authors: Zheng J, Qi S, Zhu H, Xiao X Abstract In the current study, we discovered that Hoechst 33342 (H342) could produce significant toxicity to the human neuroblastoma SK-N-SH cells at concentrations typically used to identify side population (SP) cells. At 3.75 and 5 µg/ml, H342 induced cell death and apoptosis. Flow cytometry analysis revealed cell cycle arrest at S-phase. Our findings caution against using H342 for SP analysis without considering differential susceptibility of different cells to H342. Specifically, concentration of H342 needs to be adjusted carefully to minimize toxicity for each cell l...
Source: Cellular and Molecular Biology - July 29, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Combined PCR and Q-RT-PCR technique for detecting chimerism in a non-human Primate vascularized osteomyocutaneous allografts model.
Authors: Jiang J, Wang J, Zhong F, Chen G, Li Y, Zheng XX Abstract Face transplantation and other composite tissue transplantation (CTA) are permissive to transplantation tolerance. The real reason, that composite tissue containing bone achieves transplantation immune tolerance more easily than the composite tissue without the bone is not clear. The chimerism may be the main mechanism in the progress of inducing the transplantation tolerance by CTA. We currently have established a non-human Primate Vascularized Osteomyocutaneous Allografts Model. To test the chimerism which comes from donor after the transplan...
Source: Cellular and Molecular Biology - July 29, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The role of apoptosis inducing factor in the apoptosis of retinal pigment epithelium cells induced by oxidative stress.
Authors: Fu Y, Tang M, Bai L, Fan Y Abstract To explore the role of apoptosis inducing factor (AIF) in the apoptosis of retinal pigment epithelium (RPE) cells induced by oxidative stress. RPE cell apoptotic models were constructed by H2O2, Annexin V/PI flow cytometry and MTT assay were used to determine the changes of RPE cell apoptosis and proliferation. The production of reactive oxygen species (ROS) and mitochondrial membrane potential were observed. QRT-PCR and western blot were used to determine the expression of AIF to evaluate whether AIF-mediated non caspase pathway participated in the RPE cell apoptosis in...
Source: Cellular and Molecular Biology - July 29, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Association between miR-499 rs3746444 and the susceptibility of hepatocellular carcinoma.
This study suggested that miR-499 rs3746444 might play an important role in the development of HCC. PMID: 27453271 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - July 29, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Investigation of biological effects of some Mannich Bases containing Bis-1,2,4- Triazole.
In this study, the effects of Mannich bases containing bis-1,2,4-triazole on the levels of in vivo malondialdehyde (MDA) and antioxidant vitamins (A, E, C) were examined in serum, livers and kidneys of rats. DA and vitamin (A, E, C) levels were determined by high performance liquid chromatography (HPLC). Antioxidant effect was investigated by determining the MDA levels in Saccharomyces cerevisiae cells as in vitro. Furthermore, the antitumor effects of compounds were investigated against MCF-7 human breast cancer cells. Interrelations of results among control and compound groups were evaluated using SPSS statistical softwa...
Source: Cellular and Molecular Biology - July 29, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Baicalein induces apoptosis and reduces inflammation in LPS-stimulated keratinocytes by blocking the activation of NF- κB: implications for alleviating oral lichen planus.
Baicalein induces apoptosis and reduces inflammation in LPS-stimulated keratinocytes by blocking the activation of NF-κB: implications for alleviating oral lichen planus. Cell Mol Biol (Noisy-le-grand). 2016;62(7):55-60 Authors: Wang J, Luo H, Yang L, Li Y Abstract ic inflammatory diseases, including OLP, involves in the activation of the nuclear factor-kappa B (NF-κB) signaling pathway. Baicalein (BAI) is an alcohol soluble flavonoid known for its anti-inflammatory effect. However, its effectiveness on keratinocytes in OLP remains unclear. In the present study, we examined inflammation in ...
Source: Cellular and Molecular Biology - July 29, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The frequency of C609T polymorphism in the NQO1 gene and its relation to cytogenetic abnormalities in patients with myelodysplastic syndrome.
Authors: Bagatir G, Sirma S, Palanduz S, Ozturk S, Cefle K, Ozbek U, Yenerel M, Nalcaci M Abstract The aim of the present study is to evaluate the frequency of C609T polymorphism in the NQO1 (NAD(P)H) quinon oxydoreductase) gene and its relation to cytogenetic abnormalities in patients with Myelodysplastic Syndrome (MDS). The study group consisted of 80 patients MDS with 13 of them in the pediatric age group. The frequency of the NQO1 gene polymorphism was compared with a healthy control group involving 423 individuals. Cytogenetic abnormalities were detected in 43 patients (54%). In patients with MDS the overall f...
Source: Cellular and Molecular Biology - July 29, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Mesenchymal stem cell-derived exosomes: A novel potential therapeutic avenue for cardiac regeneration.
Authors: Safari S, Malekvandfard F, Babashah S, Alizadehasl A, Sadeghizadeh M, Motavaf M Abstract Coronary artery diseases (CADs) represent a significant cause of death worldwide. During recent decades the rate of cardiovascular mortality has been declined as a result of modern medicine and surgery. However, despite the fact that cardiac cells, including cardiomyocytes (CMCs), vascular smooth muscle cells (VSMC) and vascular endothelial cells (VEC), can be regenerated by cardiac adult stem cell, the regenerative capacity of these cells are limited and inadequate to functionally regenerate heart damaged tissue. Thus...
Source: Cellular and Molecular Biology - July 29, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Therapeutic application of mesenchymal stem cell-derived exosomes: A promising cell-free therapeutic strategy in regenerative medicine.
Authors: Motavaf M, Pakravan K, Babashah S, Malekvandfard F, Masoumi M, Sadeghizadeh M Abstract Mesenchymal stem cells have emerged as promising therapeutic candidates in regenerative medicine. The mechanisms underlying mesenchymal stem cells regenerative properties were initially attributed to their engraftment in injured tissues and their subsequent transdifferentiation to repair and replace damaged cells. However, studies in animal models and patients indicated that the low number of transplanted mesenchymal stem cells localize to the target tissue and transdifferentiate to appropriate cell lineage. Instead the ...
Source: Cellular and Molecular Biology - July 29, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Norepinephrine and acetylcholine changes during electrically-induced atrial fibrillation episodes in canine models.
In conclusion, electrically-induced AF initiated by various modalities of stimulation showed different changes in serum and RA tissues. Fast frequency pacing caused significant atrial electrical remodeling, including ANS activity change. PMID: 27453277 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - July 29, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Molecular detection and in vitro antioxidant activity of S-allyl-L-cysteine (SAC) extracted from Allium sativum.
Authors: Sun YE, Wang WD Abstract It is well known that Allium sativum has potential applications to clinical treatment of various cancers due to its remarkable ability in eliminating free radicals and increasing metabolism. An allyl-substituted cysteine derivative - S-allyl-L-cysteine (SAC) was separated and identified from Allium sativum. The extracted SAC was reacted with 1-pyrenemethanol to obtain pyrene-labelled SAC (Py-SAC) to give SAC fluorescence properties. Molecular detection of Py-SAC was conducted by steady-state fluorescence spectroscopy and time-resolved fluorescence method to quantitatively measure c...
Source: Cellular and Molecular Biology - July 29, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Frequency Evaluation of T6235C (m1) and A4889G (m2) Polymorphisms of CYP1A1 Gene in a Healthy Population from the west of Mazandaran Province, Iran.
Authors: Ahangar N, Alizadeh B, Tousi A Abstract CYP1A1 is an important phase I xenobiotic metabolizing enzyme involved in the metabolism of numbers of toxins, endogenous hormones and drugs. Polymorphisms in this phase I gene can alter enzyme activity and induction, also are known to be associated with cancer susceptibility related to environmental toxins and hormone exposure. The present study was aimed to determine the frequencies of commonly known functional polymorphismsof CYP1A1 gene including CYP1A1 m1 (MspI), and CYP1A1 m2 (Ile-Val) in a healthy population from the west of Mazandaran province, Iran. A total ...
Source: Cellular and Molecular Biology - July 29, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Knockdown of eIF3a ameliorates cardiac fibrosis by inhibiting the TGF- β1/Smad3 signaling pathway.
Knockdown of eIF3a ameliorates cardiac fibrosis by inhibiting the TGF-β1/Smad3 signaling pathway. Cell Mol Biol (Noisy-le-grand). 2016;62(7):97-101 Authors: Li B, Chen H, Yang X, Wang Y, Qin L, Chu Y Abstract Cardiac fibroblasts are key effector cells in the pathogenesis of cardiac fibrosis. The eukaryotic translation initiation factor (eIF) 3a is the largest subunit of the eIF3 complex and has been involved in renal fibrosis. However, the precise role of eIF3a in myofibroblast differentiation and cardiac fibrosis remains unknown. Accordingly, in our present study, we tested the expression of eIF3...
Source: Cellular and Molecular Biology - July 29, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Pharmacogenetics of drug metabolizing enzyme: thiopurine methyl transferase phenotypes and multidrug resistance 1 gene polymorphism in inflammatory bowel disease.
Authors: Bahrehmand F, Kiani A, Vaisi-Raygani A, Bashiri H, Zobeiri M, Tanhapour M, Pourmotabbed T Abstract Inflammatory bowel disease(IBD) is progressing rapidly in developing countries such as Iran. This research is intended to compile the frequency distribution of the drug metabolizing enzyme, thiopurine methyl transferase(TPMT) and the drug transporter, Multi drug resistance(MDR1) which are involved in metabolism of many therapeutics such as thiopurines in inflammatory bowel disease(IBD). Ethnicity is an important variable influencing drug response. The aims of this research were to investigate the association...
Source: Cellular and Molecular Biology - July 29, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

How sesquiterpenes modulate signaling cascades in cancers.
Authors: Jabeen S, Qureshi MZ, Attar R, Aslam A, Kanwal S, Khalid S, Qureshi JM, Aras Perk A, Farooqi AA, Ismail M Abstract Data obtained from high-throughput technologies has started to shed light on the interplay between signal transduction cascades and chromatin modifications thus adding another layer of complexity to the already complex regulation of the protein network. Based on the insights gleaned from almost a decade of research, it has now been convincingly revealed that sesquiterpenes effectively modulated different intracellular signaling cascades in different cancers. In this review we summarize how ses...
Source: Cellular and Molecular Biology - July 29, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Induction of  linalool as a pharmaceutical and medicinal metabolite via cell suspension culture of cumin (Cuminum cyminum L.).
Induction of linalool as a pharmaceutical and medicinal metabolite via cell suspension culture of cumin (Cuminum cyminum L.). Cell Mol Biol (Noisy-le-grand). 2016;62(6):65-68 Authors: Kazemi N, Kahrizi D, Mansouri M, Karim H, Vaziri S, Zargooshi J, Khanahmadi M, Shokrinia M, Mohammadi N Abstract Cumin is an important medicinal plant in Iran. Plant cell suspension culture is a method for the production of medicinal and secondary metabolites. The linalool is a plant secondary metabolite that has been recognized as a neuroprotective agent. The purpose of this study was to evaluate the effects of sali...
Source: Cellular and Molecular Biology - July 22, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Usnea cornuta K örb. and Usnea glabrescens (Vainio) Vainio sens. Lat.: Species to be protected in the peninsula of Edough (Algeria).
Usnea cornuta Körb. and Usnea glabrescens (Vainio) Vainio sens. Lat.: Species to be protected in the peninsula of Edough (Algeria). Cell Mol Biol (Noisy-le-grand). 2016;62(6):69-73 Authors: Fekroune Chaker A Abstract In the Mediterranean region, Edough Peninsula (Algerian Northeast) has a significant procession of rare plant species biogeographical interest. This communication is based on specimens collected by the author during the realization of a lichen herbarium when her attention was attracted by the striking presence of a fruticose lichen of Usnea genus, on a hawthorn at the entrance of a pr...
Source: Cellular and Molecular Biology - July 22, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Knockdown of eIF3a attenuates the pro-fibrogenic response of hepatic stellate cells induced by TGF- β1.
Knockdown of eIF3a attenuates the pro-fibrogenic response of hepatic stellate cells induced by TGF-β1. Cell Mol Biol (Noisy-le-grand). 2016;62(6):107-111 Authors: He P, Yu ZJ, Sun CY, Jiao SJ, Jiang HQ Abstract Activation of hepatic stellate cells (HSCs) plays an important role in the development of liver fibrosis. The eukaryotic translation initiation factor (eIF) 3a is the largest subunit of the eIF3 complex and has been involved in pulmonary fibrosis. However, the role of eIF3a in liver fibrosis remains largely unknown. Therefore, in this study, we investigated the role of eIF3a in transforming...
Source: Cellular and Molecular Biology - July 22, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Epicardial adipose tissue volume a diagnostic study for independent predicting disorder of circadian rhythm of blood pressure in patients with essential hypertension.
Authors: Zhou L, Deng Y, Gong J, Chen X, Zhang Q, Wang J Abstract The aim of the study was to determine whether epicardial adipose tissue volume (EATV), a new cardiometabolic risk factor, is associated with circadian changes of blood pressure (BP) in patients with newly diagnosed essential hypertension. Ninety patients with newly diagnosed essential hypertension underwent ambulatory blood pressure monitoring for 24 h. EATV was measured using cardiac computed tomography. These patients were categorized into three groups according to their BP patterns (group 1, n=46, dipper hypertension, also called normal pattern; g...
Source: Cellular and Molecular Biology - June 7, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Mutagenic, antimutagenic, antioxidant, anti-lipoxygenase and antimicrobial activities of Scandix pecten-veneris L.
In this study S. pecten-veneris leaves were shade dried, powdered and extracted with methanol. The purpose of this study was to assay the in vitro mutagenic, antimutagenic, antioxidant, antilipoxygenase and antimicrobial activities of S. pecten-veneris leaf extract. The methanolic extract indicated no mutagenicity when tested with Salmonella typhimurium strains TA98 and TA100. Antimutagenic activity was reported with inhibition of mutagenicity in a concentration dependent fashion. The methanolic extract demonstrated antioxidant activity in the DPPH radical-scavenging test (IC50 = 4.57 mg/mL), comparable to ascorbic acid an...
Source: Cellular and Molecular Biology - June 7, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Mapping of protein-protein interaction network of Alexander disease.
This study was aimed at getting a better insight into ALXD pathogenesis and identifying the important functional and highly interconnected nodes in human protein interaction network, identifying the important sub-networks in the system could be helpful in understanding the underlying molecular mechanism. The topological analysis of human protein interaction network strategy to identify highly interconnected sub-network modules from which six proteins are found i.e. GFAP, PLEC, CRYAB, NDUFV1, CASP3 and MAPK14 plays important role in disease. Further, the enrichment analysis of interaction network identifies crucial pathways...
Source: Cellular and Molecular Biology - June 7, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research