Circular RNA circ_0096041 promotes osteosarcoma cell proliferation and migration via sponging miR-556-5p and regulating LIN28A expression
Cell Mol Biol (Noisy-le-grand). 2024 Feb 29;70(2):113-119. doi: 10.14715/cmb/2024.70.2.16.ABSTRACTStrategies targeting lin-28 homolog A (LIN28A) for the treatment of osteosarcoma are limited, even though salient findings have illustrated the crucial role of LIN28A in bone deformities and cancer. In the present study, we proved circ_0096041, one of the circular RNAs (circRNAs) with significant upregulated expression in osteosarcoma, to be notably engaged in the progression of osteosarcoma. We elucidated that osteosarcoma patients with highly expressed circ_0096041 had relatively worse prognoses. We determined that circ_0096...
Source: Cellular and Molecular Biology - March 2, 2024 Category: Molecular Biology Authors: Fulin Zhou Hao Wu Zhen Yin Weibo Zhou Wei Chen Source Type: research
Bone mesenchymal stem cell-derived exosomal miR-26a-3p promotes autophagy to attenuate LPS-induced apoptosis and inflammation in pulmonary microvascular endothelial cells
This study aimed to explore the treatment effect and regulatory mechanism of bone mesenchymal stem cell-derived exosomes (BMSC-expos) on ALI. PMVECs were stimulated by Lipopolysaccharide (LPS) to imitate ALI environment. Cell viability was determined by CCK-8 assay. Cell apoptosis was evaluated by TUNEL and flow cytometry. ELISA was utilized for testing the contents of TNF-α, IL-1β, IL-6, and IL-17. Western blot was applied for testing the levels of autophagy-related proteins LC3, p62, and Beclin-1. RNA interaction was determined by luciferase reporter assay. The ALI rat model was established by intratracheal injection o...
Source: Cellular and Molecular Biology - March 2, 2024 Category: Molecular Biology Authors: Qianfei Wang Yiming Huang Zherui Fu Source Type: research
miR-378a-5p represses Barrett's esophagus cells proliferation, migration and invasion through targeting TSPAN8
Cell Mol Biol (Noisy-le-grand). 2024 Feb 29;70(2):97-103. doi: 10.14715/cmb/2024.70.2.14.ABSTRACTBarrett's esophagus (BE) belongs to a pathological phenomenon occurring in the esophagus, this paper intended to unveil the underlying function of miR-378a-5p and its target TSPAN8 in BE progression. GEO analysis was conducted to determine differentially expressed genes in BE samples. Non-dysplastic metaplasia BE samples, high-grade dysplastic BE samples and controls were collected from subjects. CP-A and CP-B cells were exposed to bile acids (BA) to mimic gastroesophageal reflux in BE cells. RT-qPCR as well as western blot wer...
Source: Cellular and Molecular Biology - March 2, 2024 Category: Molecular Biology Authors: Xudong Xie Duo Wei Source Type: research
Green synthesis and characterization of silver nanoparticles from Ducrosia flabellifolia Boiss. aqueous extract: Anti-quorum sensing screening and antimicrobial activities against ESKAPE pathogens
Cell Mol Biol (Noisy-le-grand). 2024 Feb 29;70(2):88-96. doi: 10.14715/cmb/2024.70.2.13.ABSTRACTBiosynthesis of silver nanoparticles using natural compounds derived from plant kingdom is currently used as safe and low-cost technique for nanoparticles synthesis with important abilities to inhibit multidrug resistant microorganisms (MDR). ESKAPE pathogens, especially MDR ones, are widely spread in hospital and intensive care units. In the present study, AgNPs using Ducrosia flabellifolia aqueous extract were synthesized using a reduction method. The green synthesized D. flabellifolia-AgNPs were characterized by UV-Vis spectr...
Source: Cellular and Molecular Biology - March 2, 2024 Category: Molecular Biology Authors: Mejdi Snoussi Ramzi Hadj Lajimi Salman Latif Walid Sabri Hamadou Mousa Alreshidi Syed Amir Ashraf Mitesh Patel Jamal R Humaidi El Hassane Anouar Adel Kadri Emira Noumi Source Type: research
Evaluation of coriander seed powder supplementation on body composition, growth performance, hematology, and serum biochemical indices in common carp (Cyprinus carpio) fingerlings
This study aimed to investigate the role of coriander seed powder (Coriandrum sativum) on growth indices, feed utilization, body composition, and haemato-biochemical parameters in common carp (Cyprinus carpio) fingerlings over 84 days. One hundred and forty-four common carp (25.24±0.05 g) were assigned into four groups fed with different diets, namely 0 (basal diet), 1%, 2%, and 4% of coriander seed powder (CSP). In the current study, C. carpio fed with dietary CSP revealed significant improvement in weight gain, final weight, specific growth rate, total feed intake, feed conversion efficiency, feed conversion ratio, prot...
Source: Cellular and Molecular Biology - March 2, 2024 Category: Molecular Biology Authors: Zena Ahmed Qader Samad Sofy Omar Source Type: research
Pan-cancer analysis reveals a regulatory pattern of anoikis in human cancers
Cell Mol Biol (Noisy-le-grand). 2024 Feb 29;70(2):51-61. doi: 10.14715/cmb/2024.70.2.8.ABSTRACTAnoikis emerges when a cell finds itself extricated from the appropriate extracellular matrix, leading to an interruption in integrin ligation and thus triggering programmed cellular demise. The cardinal role of Anoikis in the realms of tumor invasion and metastasis is undeniable, although our grasp on its precise influence within the convoluted landscape of cancer biology remains somewhat circumscribed. Notably, both the immune milieu of the tumor and its inherent aggression are correlated with the fluctuating variables of Anoik...
Source: Cellular and Molecular Biology - March 2, 2024 Category: Molecular Biology Authors: Xiaoyun Wang Chao Yang Xingjian Wang Pengfei Duan Source Type: research
Protective effect of exosomes derived from bone marrow mesenchymal stem cells on hypoxia reperfusion injury of cardiomyocytes
Cell Mol Biol (Noisy-le-grand). 2024 Feb 29;70(2):73-80. doi: 10.14715/cmb/2024.70.2.10.ABSTRACTWe aimed to investigate the cardiomyocyte-protective effects of bone marrow mesenchymal stem cells (BMSCs)-derived exosomes on ischemia/reperfusion (I/R)-injured rats and to explore the mechanisms. Cardiomyocytes were divided into control group, ischemia/reperfusion group (I/R group), ischemia/reperfusion+exosome group (I/R+Exo group) or ischemia/reperfusion+exosomes transfected with miR-101a-3p inhibitor group (I/R+Exo inhibitor group). MiR-101a-3p levels were lower in I/R and I/R+Exo inhibitor groups than in control and I/R+Ex...
Source: Cellular and Molecular Biology - March 2, 2024 Category: Molecular Biology Authors: Di Zhao Yanling Bu Haifeng Shao Jian Wang Wenhua Li Qiang Li Source Type: research
Sirtuin 3 (SIRT3) improves sevoflurane-induced postoperative cognitive impairment by regulating mitochondrial oxidative stress
Cell Mol Biol (Noisy-le-grand). 2024 Feb 29;70(2):62-66. doi: 10.14715/cmb/2024.70.2.9.ABSTRACTOne of the most prevalent co-operative disorders is postoperative cognitive dysfunction (POCD), however, its pathogenesis remains unclear. Thus, the aim of this work was to evaluate SIRT3's impact on cognitive decline in aged mice under anesthesia. Adeno-associated virus SIRT3 vector (AAV-SIRT3) or empty vector (AAV-VEH) was injected into the hippocampal region of aged mice after sevoflurane induction in order to upregulate the expression of SIRT3. The expression levels of SIRT3, pro-inflammatory cytokines, and apoptotic factors ...
Source: Cellular and Molecular Biology - March 2, 2024 Category: Molecular Biology Authors: Changzong Dai Changquan Fu Zhiguo Yi Xiaohong Guan Jiandong Deng Source Type: research
Correlation between PD-1 and sPD-L1 expression levels in peripheral blood of DLBCL patients and their clinicopathological characteristics
The objectives of this study are to investigate the correlation between the expression levels of PD-1 and soluble PD-L1 (sPD-L1) in the peripheral blood of DLBCL patients, analyze their clinicopathological characteristics, and identify the optimal beneficiary group for PD-1/PD-L1 blockade. Peripheral blood samples were collected from 36 DLBCL patients before their initial treatment at Shandong Cancer Hospital between December 2018 and July 2019. The expression levels of PD-1 and sPD-L1 were measured using flow cytometry and enzyme-linked immunosorbent assay (ELISA), respectively. The clinicopathological characteristics, in...
Source: Cellular and Molecular Biology - March 2, 2024 Category: Molecular Biology Authors: Liang Wang Congcong Cao Juan Qiu Jianing Zhang Qiang He Lihua Song Linna Xie Ji Ma Source Type: research
Expression patterns and prognostic role of m6A RNA methylation regulators in Non-small Cell Lung Cancer
Cell Mol Biol (Noisy-le-grand). 2024 Feb 29;70(2):67-72. doi: 10.14715/cmb/2024.70.2.11.ABSTRACTTo investigate the expression pattern and prognostic role of m6A RNA methylation regulators in non-small cell lung cancer (NSCLC), we downloaded data from 422 patients from The Cancer Genome Atlas (TCGA) database. The relationship between the expression levels of m6A RNA methylation regulators and clinicopathological variables of NSCLC was analysed using R language. By analysing glioma data in TCGA, we found that a prognostic risk score model could be constructed based on 18 genes with m6A methylation modification. m6A gene alte...
Source: Cellular and Molecular Biology - March 2, 2024 Category: Molecular Biology Authors: Ming Zhang Congbo Yang Wen Dong Yutao Zhao Nan Chen Change Gao Source Type: research
Exploring the prognostic significance of β-HCG levels in cervicovaginal secretions and maternal risk factors for early birth
Cell Mol Biol (Noisy-le-grand). 2024 Feb 29;70(2):38-43. doi: 10.14715/cmb/2024.70.2.6.ABSTRACTPreterm birth is one of the leading causes of death in the perinatal period, this study was conducted to investigate the predictive value of β-HCG Levels in cervicovaginal secretions and maternal risk factors in preterm delivery. This cross-sectional study was conducted over a six-month period from January 1 to June 30, 2021, in Baghdad hospitals. The data were collected and used from the mothers who went to the hospital for delivery. Demographic information of patients and some risk factors were investigated. Vaginal secretions...
Source: Cellular and Molecular Biology - March 2, 2024 Category: Molecular Biology Authors: Homood Alharbi Abdualrahman Saeed Alshehry Ahmed Alsadoun Mohammad Ahmad Mustafa Sawsan Aloahd Source Type: research
METTL3-mediated m6A modification enhances ZDHHC16 expression in nonsmall- cell lung cancer patients, attenuating ferroptosis by suppressing CREB ubiquitination
Cell Mol Biol (Noisy-le-grand). 2024 Feb 29;70(2):30-37. doi: 10.14715/cmb/2024.70.2.5.ABSTRACTAt present, the early diagnosis and treatment of non-small cell lung cancer (NSCLC) is still an urgent problem to be solved worldwide, including in China. The present work investigated the possible protective effect of ZDHHC16 in cell proliferation and metastasis of NSCLC and explored its possible mechanisms. ZDHHC16 expression level in patients with Non-Small-Cell Lung Cancer was up-regulation. ZDHHC16 gene is stabilized by m6A methylation. ZDHHC16 gene reduced ferroptosis of NSCLC by the rehabilitation of the mitochondrial stru...
Source: Cellular and Molecular Biology - March 2, 2024 Category: Molecular Biology Authors: Zeyu Liu Chuanqing Jing Wei Zhang Source Type: research
Association of a single-nucleotide polymorphism in C12orf43 region with the risk of coronary artery disease
In this study based on the genetic approach to dyslipidemia, a total of 200 subjects were included from the southern Punjab. The biochemical analysis of parameters (total cholesterol, triglycerides, blood glucose, high-density lipoprotein, and low-density lipoprotein) was carried out along with molecular analysis using an ARMS-PCR-based assay for single-nucleotide polymorphism (SNP) C12orf43/rs2258287 to identify the genotype. Genotypes showed a substantial correlation with both family history and metabolic markers. The cholesterol, low-density lipoprotein cholesterol (LDL-C), triglycerides and blood glucose levels were hi...
Source: Cellular and Molecular Biology - March 2, 2024 Category: Molecular Biology Authors: Najma Qammar Maryam Zain Raheela Jabeen Farah Deeba Nadia Iqbal Hafiz Muhammad Rashad Javeed Fatema Suliman Alatawi Mohsen Suliman Alatawi Sanaa Almowallad Amnah A Alharbi H üseyin Şahin Source Type: research
Diagnostic advancements: Isolating Mycobacterium avium ssp. paratuberculosis and unveiling its molecular identity with nested-PCR
Cell Mol Biol (Noisy-le-grand). 2024 Feb 29;70(2):18-23. doi: 10.14715/cmb/2024.70.2.3.ABSTRACTMycobacterium avium subspecies paratuberculosis (MAP) is the causative agent of paratuberculosis, which is currently prevalent in many parts of Iran and produces severe economic loss. It is hence necessary to identify and isolate the animals infected with this bacterium, so this research aimed to isolate MAP from milk and fecal samples of ELISA-positive animals and determine the molecular identity of isolates. After performing ELISA on 3,700 bovine blood samples, 115 samples of milk and feces were taken from ELISA-positive cattle...
Source: Cellular and Molecular Biology - March 2, 2024 Category: Molecular Biology Authors: Faranak Nouri Alireza Shahrjerdi Hossein Ali Zarnegarpour Nader Mosvari Source Type: research
Genome-wide association studies of salinity tolerance in local aman rice
Cell Mol Biol (Noisy-le-grand). 2024 Feb 29;70(2):10-17. doi: 10.14715/cmb/2024.70.2.2.ABSTRACTThe present study aimed to identify and characterize new sources of salt tolerance among 94 rice varieties from varied geographic origins. The genotypes were divided into five groups based on their morphological characteristics at both vegetative and reproductive stages using salinity scores from the Standard Evaluation System (SES). The experiment was designed as per CRD (Completely Randomized Design) with 2 sets of salinity treatments for 8 dS/meter and 12 dS/meter, respectively compared with one non-salinized control set. Usin...
Source: Cellular and Molecular Biology - March 2, 2024 Category: Molecular Biology Authors: Nusrat Jahan Mohammad Sharif Raihan M Moshiul Islam Farzana Mustafa Era Adel I Alalawy Awatif M E Omran Yasmene F Alanazi Mohamed Sakran Abdulrahman Alasmari Fahad M Alzuaibr Ayman El Sabagh Danial Kahrizi A K M Aminul Islam Source Type: research
