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Thymoquinone induces apoptosis and increase ROS in ovarian cancer cell line.
In this study, the anticancer properties of thyomquinone (TQ), the active ingredient of N. sativa, were studied using ovarian cancer cell line (Caov-3 cells). The anti-proliferative activity of TQ was determined using MTT and the apoptosis was investigated using Flowcytometry and Annexin-V Assays. Multiparameteric cytotoxicity bioassays were used to quantify the changes in cell permeability and mitochondrial membrane potential. Reactive oxygen species (ROS) and apoptosis-involved cell markers were examined to verify cell death mechanism. The MTT-assay showed that TQ induces anti-proliferative activity on Caov-3 with an IC5...
Source: Cellular and Molecular Biology - June 7, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Human amniotic fluid derived mesenchymal stem cells cause an anti-cancer effect on breast cancer cell line in vitro.
Authors: Ghafarzadeh M, Eatemadi A, Fakhravar Z Abstract Human amniotic fluid stem cells (hAFSCs) have the ability to self-renew, and multipotent differentiation into three germ layer cells. We obtained 5 ml amniotic fluid from ten 16-20 week pregnant women undergoing amniocentesis. hAFSCs were isolated from all samples, co-cultured with T47D breast cancer cell line and characterized using flow cytometry and RT-PCR. After 3, 4 and 5 days, T47D and HSFCs viability were evaluated with MTT assay. After 5 days of co-culture T47D cells viability were decreased. Our findings showed that hAFSCs can release soluble factors...
Source: Cellular and Molecular Biology - June 7, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Knockdown of eIF3a attenuates the pro-fibrogenic response of hepatic stellate cells induced by TGF-β1.
Knockdown of eIF3a attenuates the pro-fibrogenic response of hepatic stellate cells induced by TGF-β1. Cell Mol Biol (Noisy-le-grand). 2016;62(6):107-111 Authors: He P, Yu ZJ, Sun CY, Jiao SJ, Jiang HQ Abstract Activation of hepatic stellate cells (HSCs) plays an important role in the development of liver fibrosis. The eukaryotic translation initiation factor (eIF) 3a is the largest subunit of the eIF3 complex and has been involved in pulmonary fibrosis. However, the role of eIF3a in liver fibrosis remains largely unknown. Therefore, in this study, we investigated the role of eIF3a in transforming...
Source: Cellular and Molecular Biology - June 7, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The effect of Morus alba leaves extract and powder on resistin levels and liver transaminase enzymes activities in diabetes.
Authors: Salemi Z, Barzin Tond S, Fallah S, Shojaii A, Seifi M Abstract The current study was designed to investigate the changes of the resistin, aspartate aminotransferase (AST), alanine aminotransferase (ALT) levels of diabetic rats after treatment with Morus alba leaves flavonoid extract (MLE) and Morus alba leaves powder (MLP). Thirty male wistar rats in five groups including control and diabetic groups were included. Diabetic groups consisted of diabetic control, sham and treated group with MLE and MLP. Type 2 diabetes was induced in rats by administration of streptozotocin (STZ) and - nicotinamide. The serum...
Source: Cellular and Molecular Biology - June 7, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

PFKFB3 potentially contributes to paclitaxel resistance in breast cancer cells through TLR4 activation by stimulating lactate production.
In this study, we sought to examine the role of 6-Phosphofructo-2-kinase (PFKFB3), a critical regulator of glycolysis, in paclitaxel resistance development. Two clones of paclitaxel resistant breast cancer cells, MCF-7RA and MCF-7RB, were established by a long term exposure of MCF-7 cells to paclitaxel. Consequently, PFKFB3 expression was found to be increased in MCF-7RA and MCF-7RB cells compared with MCF-7 cells. Silencing PFKFB3 expression markedly reduced the IC50 concentrations of MCF-7RA and MCF-7RB cells. Moreover, PFKFB3 modulated toll like receptor 4 (TLR4) and MyD88 expression as well as interleukin (IL)-6 and IL...
Source: Cellular and Molecular Biology - June 7, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

G2013 modulates TLR4 signaling pathway in IRAK-1 and TARF-6 dependent and miR-146a independent manner.
Authors: Hajivalili M, Pourgholi F, Majidi J, Aghebati-Maleki L, Movassaghpour AA, Samadi Kafil H, Mirshafiey A, Yousefi M Abstract Inflammation is inseparable part of different diseases especially cancer and autoimmunity. During inflammation process toll like receptor 4(TLR4) responds to lipopolysaccharide (LPS), one of the bacterial components, and TLR4 signaling leads to interleukine-1 receptor associated kinase-1 (IRAK1) and tumor necrosis factor (TNF) receptor associated factor6 (TRAF6) activation which ultimately results in nuclear factor- ĸB (NF-ĸB) activation as the main transcription factor of inflammato...
Source: Cellular and Molecular Biology - May 19, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Overexpression of X chromosome-linked inhibitor of apoptosis by inhibiting microRNA-24 protects periodontal ligament cells against hydrogen peroxide-induced cell apoptosis.
Authors: Liu C, Chen Z, Wang J, Hu H Abstract Hydrogen peroxide (H2O2), a common oral clinical drug for the tooth bleaching, induces severe cell apoptosis of periodontal ligament cells (PDLCs). The excessive cell apoptosis of PDLCs impairs periodontal tissue damage and repair. However, the underlying mechanism is incompletely understood. Here, we showed that microRNA-24 (miR-24) played an important role in regulating H2O2-induced cell apoptosis of PDLCs. We found that miR-24 expression was increased in PDLCs in response to H2O2 treatment. Down-regulation of miR-24 obviously rescued H2O2-induced cell apoptosis in PD...
Source: Cellular and Molecular Biology - May 19, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Cell-free fetal DNA in amniotic fluid supernatant for prenatal diagnosis.
Authors: Soltani M, Nemati M, Maralani M, Estiar MA, Andalib S, Fardiazar Z, Sakhinia E Abstract In widespread conviction, amniotic fluid is utilized for prenatal diagnosis. Amniotic fluid supernatant is usually discarded, notwithstanding being a good source of fetal DNA. The aim of the present study was to assess cell-free fetal DNA extracted from amniotic fluid supernatant for application in prenatal diagnosis such as gender determination and early diagnosis of β-thalassemia. Samples of amniotic fluid of 70 pregnant women were collected and went through routine tests along with tests for cell-free fetal DNA ...
Source: Cellular and Molecular Biology - May 19, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Perfluorocarbon inhibits lipopolysaccharide-induced macrophage inflammatory protein-2 expression and activation of ATF-2 and c-Jun in A549 pulmonary epithelial cells.
Authors: Hu Y, Li CS, Li YQ, Liang Y, Cao L, Chen LA Abstract The signaling pathway that mediates the anti-inflammatory effects of perfluorocarbon (PFC) in alveolar epithelial cells treated with lipopolysaccharide (LPS) remains unclear. To evaluate the role of macrophage-inflammatory protein-2 (MIP-2), four A549 treatment groups were utilized: (1) untreated control, (2) 10 μg/mL of LPS, (3) 10 μg/mL of LPS+30% PFC and (4) 30% PFC. MIP-2 mRNA expression was determined by qPCR and ELISA. Mitogen-activated protein kinase (MAPK) activation was determined by Western blot analysis, and MIP-2 expression was determin...
Source: Cellular and Molecular Biology - May 19, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The role of Iron on breast cancer stem-like cells.
In this study, therefore, iron dependency in HER2 overexpression and CSC survival is examined in breast cancer cell line, MCF7. It has shown that cells overexpressing HER2 require iron more than their vector counterparts and HER2-increased CSCs are vulnerable to iron chelation. Additionally, this sensitivity of CSCs to iron reduction is obviously indicated in various breast cancer cell lines; HCC1954, MDA-MB-435 and Hs578T. Finally, the concept is also shown in neoplastically transformed breast cancer cell line, HMLER. Altogether, this study demonstrates that iron depletion causes toxicity for CSCs. PMID: 27188730 [Pu...
Source: Cellular and Molecular Biology - May 19, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Detection of the novel IL-1 family cytokines by QAH-IL1F-1 assay in rheumatoid arthritis.
In this study, we detected serum levels of IL-36 subfamily cytokines (including IL-36α, IL-36β, IL-36γ, IL-36Ra and IL-38), IL-37, IL-33 and aimed to investigate the roles of these cytokines in rheumatoid arthritis (RA) preliminarily. A total of 10 RA patients and 10 healthy controls (HCs) were involved in this study, we measured IL-36 subfamily cytokines, IL-37 and IL-33 levels in the serum of the experiment subjects by QAH-IL1F-1 assay. Clinical and laboratory data of the subjects were collected and analyzed by Spearman's rank test. Compared to that of HCs, IL-36α, IL-36β, IL-36Ra, IL-38 and I...
Source: Cellular and Molecular Biology - May 19, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Enhancing immune responses of EV71 VP1 DNA vaccine by co-inoculating plasmid IL-12 or GM-CSF expressing vector in mice.
This study provides a frame of reference for the design of DNA vaccines against EV71. PMID: 27188732 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - May 19, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The roles of SPBC409.08 and SPAC9.02c hypothetical genes in cell cycle and stress response, in Schizosaccharomyces pombe.
In this study, our aim is to characterize two hypothetical Schizosaccharomyces pombe genes, SPBC409.08 and SPAC9.02c, which show sequence similarity to spermine family transporters and polyamine N-acetyltransferases, respectively. To this end, we generated deletion mutants of SPBC409.08 and SPAC9.02c genes using Bahler method and checked the cell cycle progression and stress responses of these mutants. Our results showed that SPBC409.08Δ cells showed some defects in the cell size, while SPAC9.02cΔ cells showed some sensitivity to UV irradiation. These data support their potential roles in the cell cycle and str...
Source: Cellular and Molecular Biology - May 19, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Efficacy of hematopoietic stem cell formultiple sclerosis, an evidence based meta-analysis.
Authors: Li C, Feng J, Chen S, He Y Abstract Multiple sclerosis (MS) is thought to be a serious autoimmune disease. However, few therapy method was efficient for MS. The hematopoietic cell transplant (HCT) has been reported for a long time and can be used for MS. The clinical trials consisted of small samples and gave confusing results. This systematic review and meta-analysis aims to estimate the effects of HCT for adults with MS. We searched the database of CNKI, PUBMED, EMBASE, WEB of SCIENCE and the Cochrane Center Register of Controlled Trials to find initial studies and selected the appropriate researches inc...
Source: Cellular and Molecular Biology - May 19, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Genetic diversity of Iranian honey bee (Apis mellifera meda Skorikow, 1829) populations based on ISSR markers.
In this study, the genetic diversity of different Iranian honey bee populations was evaluated using inter simple sequence repeat (ISSR) markers. During May to September 2014, 108 young worker honey bees were collected from six different populations in 30 different geoclimatic locations from Golestan, Mazendaran, Guilan, West Azerbaijan, East Azerbaijan, Ardebil provinces of Iran. DNA was extracted from the worker honey bees. The quality and quantity of extracted DNA were measured. A set of ten primers were screened with the laboratory populations of honey bees. The number of fragments produced in the different honey bee po...
Source: Cellular and Molecular Biology - May 19, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Expression and significance of aquaporin protein in sprague-dawley rats after experimental intraventricular hemorrhage.
Authors: Gu W, Li F, Zhang W, Jia P Abstract The AQP4 protein in Sprague-Dawley (SD) rats was expressed after experimental intraventricular hemorrhage to investigate the possible pathogenesis of chronic posthemorrhagic hydrocephalus. Seventy SD rats were randomly divided into two groups - sham-operated group (n=35) and experimental group (n=35). Saline and citrated autologous blood were injected into the lateral ventricle of the SD rats in the two groups, respectively. The rats in experimental group were further divided into seven subgroups based on different time points at 3 h, 6 h, 12 h, 1 d, 3 d, 5 d and 7 d. Th...
Source: Cellular and Molecular Biology - May 19, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effect of ketamine anesthesia on cognitive function and immune function in young rats.
Authors: Sun YT, Hou M, Zou T, Liu Y, Liu J, Wang YL Abstract The aim of the current study was to explore the effect of the ketamine on the immune function and cognitive function in young rats. The young rats (40) rats were randomly divided into two groups where each group contains 20 rats, such as Group I: normal control; Group II: Ketamine treated group. All group rats received the intravenous injection of treatment for three times and the hippocampal neuronal apoptosis and the immune parameters such as IL-2, IL-4 and IL-10 and whole brain IL-1β level were estimated. The cognitive ability effect of the young...
Source: Cellular and Molecular Biology - May 19, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Molecular and characterization of NnPPO cDNA from lotus (Nelumbo nucifera) in rhizome browning.
Authors: Dong C, Yu AQ, Yang MG, Zhou MQ, Hu ZL Abstract The complete cDNA (NnPPO) of polyphenol oxidase in Nelumbo nucifera was successfully isolated, using Rapid amplification cDNA end (RACE) assays. The full-length cDNA of NnPPO was 2069 bp in size, containing a 1791 bp open reading frame coding 597 amino acids. The putative NnPPO possessed the conserved active sites and domains for PPO function. Phylogenetic analysis revealed that NnPPO shared high homology with PPO of high plants, and the homology modeling proved that NnPPO had the typical structure of PPO family. In order to characterize the role of NnPPO, Re...
Source: Cellular and Molecular Biology - May 19, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Expression and variability of lipid metabolism genes in intracranial aneurysm.
The objective of this study was to investigate the association between mRNA expression and single nucleotide polymorphisms (SNPs) of the ATP-binding cassette transporter (ABCA1) gene, apolipoprotein A1 (APOA1) gene, low-density lipoprotein (LDLR) gene and RNA gene located in the CDKN2B-CDKN2A cluster (CDKN2B-AS1) involved in lipid metabolism and the occurrence of intracranial aneurysm (IA). Fifty three IA patients, and 27 controls (IA-free) were enrolled in this study and were genotyped for seven single nucleotide polymorphisms. Increased expression of the LDLR gene in IA patients was observed. The A/G genotype and the A a...
Source: Cellular and Molecular Biology - May 19, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Amyloid beta directed antibody for Alzheimer's disease, an evidence based meta-analysis.
In conclusion, Antibody was not benefit for AD based on MMSE and CDR-SB but had a little effect according to ADAS-cog. PMID: 27188740 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - May 19, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Meta-analysis of traditional herbal medicine in the treatment of nonalcoholic fatty liver disease.
Authors: Peng H, He Y, Zheng G, Zhang W, Yao Z, Xie W Abstract Traditional Chinese herbal medicine (TCM) has been found effective for nonalcoholic fatty liver disease (NAFLD) based on its unique theory system and substantial herb remedies. The aim of this meta-analysis was to evaluate the efficiency and safety of one of the TCM, Danshen in the treatment of NAFLD. Seven English and Chinese databases were searched from inceptions to December 2015. RCTs which compared Danshen with placebo in adult patients with NAFLD were included. Totally, 8 RCTs with a total of 800 patients were identified. The results showed that c...
Source: Cellular and Molecular Biology - May 19, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

In Vitro and in Vivo antioxidant activity of ethyl acetate extraction of purple rice.
Authors: Shen Y, Chen L, Zhang H, Zhang Y, Zhang H, Wang L, Qian H, Qi X, Zhang H Abstract The antioxidant activities of ethyl acetate extraction of purple rice (EAEPR) were evaluated by various methods in vitro and in vivo. In in vitro antioxidant assays, EAEPR was found to have strong 2, 2-diphenyl-1-picrylhydrazyl radical scavenging activity hydroxyl radical, reducing power and metal-ion chelating activity. In in vivo antioxidant assays, mice were administered with EAEPR via gavage for 42 consecutive days. As a result, administration of EAEPR significantly enhanced the activities of glutathione peroxidase in ser...
Source: Cellular and Molecular Biology - May 19, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Hsa-miRNA-31 regulates epithelial cell barrier function by inhibiting TNFSF15 expression.
Authors: Nan X, Qin S, Yuan Z, Li Y, Zhang J, Li C, Tan X, Yan Y Abstract Ulcerative colitis (UC) is characterized by epithelial barrier disruption and alterations in immune regulation but with the etiology unknown. MicroRNA-31 is the most consistent differentially expressed miRNA in ulcerative colitis tissue. The aim of this project is to study the important roles of miRNA-31 in regulation of intestinal epithelial barrier function. We found that expression of miRNA-31 is proportional to the proliferation of Caco2-BBE cells and overexpression of miRNA-31 can increase its trans-epithelial resistance (TER) by decreas...
Source: Cellular and Molecular Biology - May 19, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The efficacy of high-intensity, focused ultrasound treatment for non-neoplastic epithelial disorders of the vulva.
Authors: Zhou W, Zhu L, Zhou H, Shen K, Lang J, Cui Q, Shi H Abstract Non-neoplastic epithelial disorders of the vulva (NNEDV) are common types of vulval lesions. Although corticosteroids represent a first-line treatment for NNEDV, concerns exist about the safety associated with long-term topical corticosteroid use. Recently, several clinical trials have identified high-intensity focused ultrasound (HIFU) as a promising treatment modality for NNEDV. The aim of this multi-center, randomized, controlled clinical trial was to investigate the efficacy of HIFU therapy in women with NNEDV based on histological alteration...
Source: Cellular and Molecular Biology - May 19, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Edible bird's nest enhances antioxidant capacity and increases lifespan in Drosophila Melanogaster.
In this study, we aims to investigate the effects of edible bird's nest (EBN) on anti-aging efficacy. In order to investigate lifespan and mortality rate of flies, we treated flies with various doses of EBN. Besides, fecundity, water content and food are determined and heat-stress test is conducted after flies treating with different medium. Effects of EBN on total antioxidant activity (T-AOC), super-oxide dismutase activity (SOD), catalase activity (CAT), and malondialdehyde (MDA) were examined in drosophila melanogaster. Results indicated that flies in EBN treated group illustrated significantly lower mortality rates and...
Source: Cellular and Molecular Biology - May 19, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Icariin induces S-phase arrest and apoptosis in medulloblastoma cells.
Authors: Sun Y, Sun XH, Fan WJ, Jiang XM, Li AW Abstract Medulloblastoma is the most common type of malignant brain tumor in children. Despite a relatively high long-term survival rate, complications still represent great burden for the majority of patients receiving traditional therapy. Therefore, the development of new effective treatments and drugs is urgently needed. A cell counting kit-8 (CCK-8) and colony formation assay were used to evaluate medulloblastoma cell proliferation and colony formation, respectively. Cell cycles and apoptosis were assessed by flow cytometry. A western blot was performed to determi...
Source: Cellular and Molecular Biology - May 19, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Editorial.
Authors: Farooqi AA Abstract To optimize treatment, we need to understand biology of different diseases in much more detail with emphasis on morphological, proteomic, genetic and epigenetic grounds. Keeping in view the facts and stimulating developments in molecular pathology, it is worthwhile to present an up-date on this topic.It is becoming progressively more understandable that exciting fields of pharmacogenomics and pharmacogenetics have revolutionized field of medicine. Better understanding of underlying mechanisms of different diseases has provided us with better ways to treat illnesses. There cannot be a di...
Source: Cellular and Molecular Biology - May 19, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The effect of glycyrrhizic acid on traumatic spinal cord injury in rats.
Authors: Sehitoglu MH, Guven M, Yüksel Y, Akman T, Bozkurt Aras A, Farooqi AA, Cosar M Abstract Spine injury associated with traumatic spinal cord injury eventuates in oxidative stress, inflammation and neuronal apoptosis. The aim of this study is to find out whether the glycyrrhizic acid treatment protects spinal cord from traumatic injuries in rats. To this end, the rats were divided into three groups: group I; control group (no drug or operation, n=8), group II; traumatic spinal cord injury group (TSCI, n=8) and group III; glycyrrhizic acid group (TSCI-GA, 80 mg/kg, n=8). Total laminectomy was performed at ...
Source: Cellular and Molecular Biology - May 19, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

The effects of Advanced Glycation End Products (RAGE)-374T/A and Gly82Ser variants and soluble-RAGE levels to obesity in children.
Authors: Kucukhuseyin O, Ozgen T, Karagedik EH, Cesur Y, Yilmaz Aydogan H, Yaylim I, Ergen HA Abstract In recent years, studies related to advanced glycation end products (AGE) and their interaction with their receptors (RAGE) have advanced our knowledge of the roles of these molecules in different diseases. However, studies concerning AGE-RAGE interaction in obesity are limited and the results are conflicting. RAGE gene is located on 6p21.3, has several polymorphic sites including -374T/A, a functional polymorphism in the promoter region, and Gly82Ser, present within the ligand-binding domain. In the present study...
Source: Cellular and Molecular Biology - May 19, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Investigation of endocrine and immunological response in fat tissue to hyperbaric oxygen administration in rats.
In conclusion, in our study we identified that HBO administration affected the endochrinological functions of fat tissue. PMID: 27188864 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - May 19, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Relationship between betatrophin levels and metabolic parameters in patients with polycystic ovary syndrome.
Authors: Erbag G, Eroglu M, Turkon H, Sen H, Binnetoglu E, Aylanc N, Asik M Abstract To evaluate the status of serum betatrophin levels and potential relations between metabolic parameters and betatrophin levels in patients with polycystic ovary syndrome. We included patients newly diagnosed with PCOS in our study. Fifty-seven female patients (30 patients with PCOS and 27 healthy control subjects) were enrolled in this study. Serum betatrophin levels were measured using a betatrophin enzyme-linked immunosorbent assay kit. Insulin resistance was calculated using the homeostasis model of the assessment-insulin resist...
Source: Cellular and Molecular Biology - May 19, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Are there possible associations between MnSOD and GPx1 gene variants for laryngeal cancer risk or disease progression?
Authors: Coskun C, Verim A, Farooqi AA, Turan S, Mezani B, Kucukhuseyin O, Tolgahan Hakan M, Ergen A, Yaylim I Abstract Laryngeal squamous cell carcinoma (LSCC) is a multifaceted and genomically complex disease and cellular and preclinical studies have demystified wide ranging molecular mechanisms which underpin its development and progression and resistance against wide ranging molecular therapeutics. Oxidative stress is a widely studied molecular mechanism and reportedly involved in carcinogenesis. Increasingly it is being realized that accumulation of Reactive Oxygen Species (ROS) activates defensive mechanism t...
Source: Cellular and Molecular Biology - May 19, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Hypoxia and laser enhance expression of SDF-1 in muscles cells.
Authors: Mirahmadi M, Ahmadiankia N, Naderi-Meshkin H, Heirani-Tabasi A, Bidkhori HR, Afsharian P, Bahrami AR Abstract Targeted homing of transplanted mesenchymal stem cells (MSCs) is a decades old discussion in regenerative medicine. It has been proved that stromal cell-derived factor-1 (SDF-1α) is a potent chemoattractant of MSCs. Therefore, different strategies have been used to increase secretion of SDF-1α in damaged tissues to elevate targeted homing of MSCs. Previous studies have revealed that increased SDF-1α expression in hypoxic necrotic tissues and also low-level laser exposure enhanced ...
Source: Cellular and Molecular Biology - May 19, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Tudor tells about new twists in the story tale of SMURFs.
Authors: Qureshi MZ, Jabeen S, Butt G, Aslam A, Naqvi SK, Attar R, Tabassum S, Nasir M, Farooqi AA Abstract Smad ubiquitin regulatory factors (SMURFS) belong to the HECT- family of E3 ubiquitin ligases. This family has two members, SMURF1 and SMURF2. SMURFs have emerged as well studied negative regulators of TGF induced intracellular signaling. However, increasingly it is being realized that SMURFs tactfully modulate an array of proteins in different cancers. This review sets spotlight on how SMURF1 and SMURF2 communicate with effectors of different signaling pathways during the multistep progression to cancer. We ...
Source: Cellular and Molecular Biology - May 19, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Adipose derived mesenchymal stem cells express keratinocyte lineage markers in a co-culture model.
Authors: Irfan-Maqsood M, Matin MM, Heirani-Tabasi A, Bahrami M, Naderi-Meshkin H, Mirahmadi M, Hassanzadeh H, Sanjar Moussavi N, Raza-Shah H, Raeesolmohaddeseen M, Bidkhori H, Bahrami AR Abstract Cutaneous wound healing is a complex type of biological event involving proliferation, differentiation, reprograming, trans/de-differentiation, recruitment, migration, and apoptosis of a number of cells (keratinocytes, fibroblasts, endothelial cells, nerve cells and stem cells) to regenerate a multi-layered tissue that is damaged by either internal or external factors. The exact regeneration mechanism of damaged skin is s...
Source: Cellular and Molecular Biology - May 19, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Ganoderma lucidum total triterpenes attenuate DLA induced ascites and EAC induced solid tumours in Swiss albino mice.
Authors: Smina TP, Mathew J, Janardhanan KK Abstract G. lucidum total triterpenes were assessed for its apoptosis-inducing and anti-tumour activities. The ability of the total triterpenes to induce apoptosis was evaluated in Dalton's lymphoma ascites (DLA) and Ehrlich's ascites carcinoma (EAC) cell lines. Total triterpenes were found to be highly cytotoxic to DLA and EAC cell lines with IC50 values 5 ± 0.32 and 7.9 ± 0.2 µg/ml respectively. Total triterpenes induced apoptosis in both cell lines which is evident from the DNA fragmentation assay. Anti-tumour activity was accessed using DLA induced...
Source: Cellular and Molecular Biology - May 19, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Anticancer activity of Cynodon dactylon and Oxalis corniculata on Hep2 cell line.
Authors: Salahuddin H, Mansoor Q, Batool R, Farooqi AA, Mahmood T, Ismail M Abstract Bioactive chemicals isolated from plants have attracted considerable attention over the years and overwhelmingly increasing laboratory findings are emphasizing on tumor suppressing properties of these natural agents in genetically and chemically induced animal carcinogenesis models. We studied in vitro anticancer activity of organic extracts of Cynodon dactylon and Oxalis corniculata on Hep2 cell line and it was compared with normal human corneal epithelial cells (HCEC) by using MTT assay. Real Time PCR was conducted for p53 and PT...
Source: Cellular and Molecular Biology - May 19, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

MicroRNA-1280 modulates cell growth and invasion of thyroid carcinoma through targeting estrogen receptor α.
MicroRNA-1280 modulates cell growth and invasion of thyroid carcinoma through targeting estrogen receptor α. Cell Mol Biol (Noisy-le-grand). 2016;62(3):1-6 Authors: Meng D, Li Z, Ma X, Fu L, Qin G Abstract Thyroid cancer (TC) is one of the most common endocrine malignancies, with a steadily increasing incidence and lethality over the last several decades. ERα is a nuclear hormone receptor that has a key role in different cellular process and participates in the development and progression of thyroid cancer. ERα is the predicted target gene of microRNA-1280 (miR-1280). The present stud...
Source: Cellular and Molecular Biology - April 13, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

In vitro induction of α-pinene, pulegone, menthol, menthone and limonene in cell suspension culture of pennyroyal (Mentha pulegium).
In vitro induction of α-pinene, pulegone, menthol, menthone and limonene in cell suspension culture of pennyroyal (Mentha pulegium). Cell Mol Biol (Noisy-le-grand). 2016;62(3):7-9 Authors: Darvishi E, Kahrizi D, Bahraminejad S, Mansouri M Abstract Medicinal plants are known as important sources of secondary metabolites. Because of the economic value of pennyroyal [Mentha pulegium L. (Lamiaceae)] in food industries, propagation of this valuable plant has special importance. Plant cell suspension culture can increase some produced components. The aim of this research was performing cell culture for...
Source: Cellular and Molecular Biology - April 13, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Detection and putative effect of GATA4 gene variants in patients with congenital cardiac septal defects.
We examined 20 patients with atrial septal defect (ASD), ventricle septal defect (VSD), atrioventricular septal defects (AVSD) and A-V canal disturbance defect and compared with examined 10 unaffected individuals as normal control. The patients were referred from Congenital Heart Disease Clinic of the Clinical Genetics department at the National Research Centre. All patients were subjected to clinical evaluation, echocardiography and karyotyping. Genomic DNA was extracted from all cases and subjected to PCR followed by direct sequencing. The predicted effect of variants was done by a variety of proper prediction tools. We ...
Source: Cellular and Molecular Biology - April 13, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Molecular detection and extraction of pyrene in plasma and tissues of Sprague-Dawley rats.
This study provides an efficient method to detect pyrene in different tissues as well as an extraction method at the molecular level, which might contribute to the development of modern molecular diagnosis and identification in vivo. PMID: 27064868 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - April 13, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Tetanus neurotoxin HCC protein commits T cells to IFN-γ producing cells.
Tetanus neurotoxin HCC protein commits T cells to IFN-γ producing cells. Cell Mol Biol (Noisy-le-grand). 2016;62(3):20-4 Authors: Hosseinimehr SJ, Torabi Goudarzi S, Hajivalili M, Hosseini M, Ghafari Khamene M, Yazdani Y, Sadreddini S, Sadreddini S, Miahipour A, Younesi V, Yousefi M Abstract A protective response against tetanus toxin and toxoid demands efficient specific T cell and B cell responses. Tetanus neurotoxin (TeNT), a 150 kDa polypeptide, is the main cause of tetanus disease. TeNT consists of two structurally distinct chains, a 50 kDa N-terminal light (L) and a 100 kDa C-terminal heavy...
Source: Cellular and Molecular Biology - April 13, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Deregulation of seven CpG island-harboring miRNAs in bladder cancer: miR-155 and miR-23b as the most promising oncomiRs.
Authors: Pençe S, Özbek E, Ozan Tiryakioğlu N, Ersoy Tunali N Abstract Analyses of differential miRNA expressions in tumor and normal tissues can identify specific miRNAs involved in cancer pathogenesis, which can then be used as diagnostic, therapeutic and prognostic biomarkers. In this respect, we aimed to investigate expression levels of seven CpG island-harboring miRNAs in 50 paired UBC tissues by qRT-PCR. miR-21 and miR-155 were found to be significantly upregulated, and miR-23b, miR-126, miR-129-5p, miR-143a and miR-218-5p were downregulated. ROC analysis indicated miR-155 as the most promising c...
Source: Cellular and Molecular Biology - April 13, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Preparation and characterization of a de-cellularized rabbit aorta as a promising scaffold in vascular tissue engineering.
In conclusion, we successfully prepared a decellularized rabbit aorta that not only largely maintains its extra-cellular structure, but also shows little toxicity. It may constitute a potential tool as a small diameter scaffold in vascular tissue engineering. PMID: 27064871 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - April 13, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Knockdown of JMJD1C, a target gene of hsa-miR-590-3p, inhibits mitochondrial dysfunction and oxidative stress in MPP+-treated MES23.5 and SH-SY5Y cells.
Authors: Wang J, Le T, Wei R, Jiao Y Abstract MicroRNAs have been shown to be closely related to many neurodegenerative disorders. The present study focuses on the role of hsa-miR-590-3p and its function in Parkinson's disease (PD). Our study showed a remarkable down-regulation of miR-590-3p expression in the 1-methyl-4 phenylpyridinium (MPP+)-treated MES23.5 and SH-SY5Y cells. Furthermore, JMJD1C was identified as a target gene of miR-590-3p in humans via the luciferase reporter assay. Our study also demonstrated that up-regulation of miR-590-3p and knockdown of JMJD1C increased the expression of peroxisome prolif...
Source: Cellular and Molecular Biology - April 13, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

DNA repair gene XPD Asp312Asn and XRCC4 G-1394T polymorphisms and the risk of autism spectrum disorder.
Authors: Dasdemir S, Guven M, Pekkoc KC, Ulucan H, Dogangun B, Kirtas E, Kadak MT, Kucur M, Seven M Abstract Autism spectrum disorder (ASD) is a complex disorder, and its extreme heterogeneity further complicates our understanding of its biology. Epidemiological evidence from family and twin studies supports a strong genetic component in ASD etiology. Oxidative stress and abnormal DNA methylation have been implicated in the pathophysiology of ASD. Brain tissues from ASD cases showed higher levels of oxidative stress biomarkers than healthy controls in postmortem analysis. Association between oxidative stress and DN...
Source: Cellular and Molecular Biology - April 13, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Alteration of N-glycoproteins/N-glycosites in human hepatic stellate cells activated with transforming growth factor-β1.
In conclusion, increased expression of N-glycoproteins and N-glycosites play important roles in cellular contractility, signal transduction, and responses to stimuli in the activated HSCs, which might provide useful information for discovering novel molecular mechanism of HSC activation and therapeutic targets in liver fibrosis. PMID: 27064874 [PubMed - in process] (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - April 13, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Monocyte chemoattractant protein-1 promoter -2518 polymorphism and susceptibility to vasculitis, rheumatoid arthritis, and multiple sclerosis: A meta-analysis.
Authors: Lee YH, Bae SC Abstract The purpose of this study was to examine whether the monocyte chemoattractant protein-1 (MCP-1) promoter -2518 A/G polymorphism (rs1024611) is associated with susceptibility to vasculitis, rheumatoid arthritis (RA), or multiple sclerosis (MS). A meta-analysis was conducted on the association between the MCP-1 -2518 A/G polymorphism and vasculitis, RA, and MS. Fourteen studies from 13 articles, including six on vasculitis, five on RA, and three on MS, consisting of 3,038 patients and 3,545 controls were available for the meta-analysis. The meta-analysis revealed no association betwee...
Source: Cellular and Molecular Biology - April 13, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Human colon cancer HT-29 cell death responses to doxorubicin and Morus Alba leaves flavonoid extract.
Authors: Fallah S, Karimi A, Panahi G, Gerayesh Nejad S, Fadaei R, Seifi M Abstract The mechanistic basis for the biological properties of Morus alba flavonoid extract (MFE) and chemotherapy drug of doxorubicin on human colon cancer HT-29 cell line death are unknown. The effect of doxorubicin and flavonoid extract on colon cancer HT-29 cell line death and identification of APC gene expression and PARP concentration of HT-29 cell line were investigated. The results showed that flavonoid extract and doxorubicin induce a dose dependent cell death in HT-29 cell line. MFE and doxorubicin exert a cytotoxic effect on huma...
Source: Cellular and Molecular Biology - April 13, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Urtica dioica dichloromethane extract induce apoptosis from intrinsic pathway on human prostate cancer cells (PC3).
Authors: Mohammadi A, Mansoori B, Aghapour M, Baradaran B Abstract Prostate cancer is considered as the major cause of death among men around the world. There are a number of medicinal plants triggering apoptosis response in cancer cells, thus have a therapeutic potential. Therefore, further studies to characterize beneficial properties of these plants in order to introduce novel anti-cancer drugs are the interest of recent researches on the alternative medicine. On the other hand, due to traditional uses and availability of Urtica dioica extract, we decided to evaluate the efficacy of this medicinal herb on pc3 pr...
Source: Cellular and Molecular Biology - April 13, 2016 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research