Comprehensive genomic features indicative for Notch responsiveness
Nucleic Acids Res. 2024 Apr 22:gkae292. doi: 10.1093/nar/gkae292. Online ahead of print.ABSTRACTTranscription factor RBPJ is the central component in Notch signal transduction and directly forms a coactivator complex together with the Notch intracellular domain (NICD). While RBPJ protein levels remain constant in most tissues, dynamic expression of Notch target genes varies depending on the given cell-type and the Notch activity state. To elucidate dynamic RBPJ binding genome-wide, we investigated RBPJ occupancy by ChIP-Seq. Surprisingly, only a small set of the total RBPJ sites show a dynamic binding behavior in response ...
Source: Cell Research - April 22, 2024 Category: Cytology Authors: Benedetto Daniele Giaimo Tobias Friedrich Francesca Ferrante Marek Bartkuhn Tilman Borggrefe Source Type: research
Redox Status as a Key Driver of Healthy Pancreatic Beta-Cells
Physiol Res. 2024 Apr 22. Online ahead of print.ABSTRACTRedox status plays a multifaceted role in the intricate physiology and pathology of pancreatic beta-cells, the pivotal regulators of glucose homeostasis through insulin secretion. They are highly responsive to changes in metabolic cues where reactive oxygen species are part of it, all arising from nutritional intake. These molecules not only serve as crucial signaling intermediates for insulin secretion but also participate in the nuanced heterogeneity observed within the beta-cell population. A central aspect of beta-cell redox biology revolves around the localized p...
Source: Cell Research - April 22, 2024 Category: Cytology Authors: B Holendov á Š Benáková M K řivonosková L Plecit á-Hlavatá Source Type: research
Mitochondrial Physiology of Cellular Redox Regulations
Physiol Res. 2024 Apr 22. Online ahead of print.ABSTRACTMitochondria (mt) represent the vital hub of the molecular physiology of the cell, being decision-makers in cell life/death and information signaling, including major redox regulations and redox signaling. Now we review recent advances in understanding mitochondrial redox homeostasis, including superoxide sources and H2O2 consumers, i.e., antioxidant mechanisms, as well as exemplar situations of physiological redox signaling, including the intramitochondrial one and mt-to-cytosol redox signals, which may be classified as acute and long-term signals. This review exempl...
Source: Cell Research - April 22, 2024 Category: Cytology Authors: P Je žek A Dlaskov á H Engstov á J Špačková J Tauber P Pr ůchová E Kloppel O Mozheitova M Jab ůrek Source Type: research
DROSHA regulates mesenchymal gene expression in Wilms tumor
This study reveals genotype-transcriptome relationships in Wilms tumor and points to ferroptosis as a potentially therapeutic vulnerability in one subset of Wilms tumor.PMID:38647377 | DOI:10.1158/1541-7786.MCR-23-0930 (Source: Cell Research)
Source: Cell Research - April 22, 2024 Category: Cytology Authors: Patricia D B Tiburcio Kavita Desai Jiwoong Kim Qinbo Zhou Lei Guo Xue Xiao Li Zhou Aysen Yuksel Daniel R Catchpoole James F Amatruda Lin Xu Kenneth S Chen Source Type: research
Promising anticancer activity of cromolyn in colon cancer: in vitro and in vivo analysis
CONCLUSION: Our study showed that cromolyn is a selective and strong drug in inhibiting the proliferation of colon cancer cells. Based on our results, the efficacy of C in vitro analysis (MTT assays and apoptosis), as well as animal studies is competitive with the FDA-approved drug doxorubicin. C is very promising as a low-complication and good-efficacy drug for cancer drug repositioning. This requires clinical research study designs to comprehensively evaluate its anti-cancer effects.PMID:38647571 | DOI:10.1007/s00432-024-05741-2 (Source: Cell Research)
Source: Cell Research - April 22, 2024 Category: Cytology Authors: Amin Aliabadi Mohammad Reza Haghshenas Razie Kiani Mohammad Reza Panjehshahin Nasrollah Erfani Source Type: research
Global profiling of protein S-palmitoylation in the second-generation merozoites of Eimeria tenella
Parasitol Res. 2024 Apr 22;123(4):190. doi: 10.1007/s00436-024-08204-2.ABSTRACTThe intracellular protozoan Eimeria tenella is responsible for avian coccidiosis which is characterized by host intestinal damage. During developmental cycle, E. tenella undergoes versatile transitional stages such as oocyst, sporozoites, merozoites, and gametocytes. These developmental transitions involve changes in cell shape and cell size requiring cytoskeletal remodeling and changes in membrane proteins, which may require transcriptional and translational regulations as well as post-translational modification of proteins. Palmitoylation is a...
Source: Cell Research - April 22, 2024 Category: Cytology Authors: Zigang Qu Yuqiong Li Wenhui Li Nianzhang Zhang Joshua Seun Olajide Xiaoyun Mi Baoquan Fu Source Type: research
Progress of Mitochondrial Function Regulation in Cardiac Regeneration
This article reviews mitochondria's multifaceted role in cardiac regeneration, particularly in myocardial infarction and heart failure models. Understanding mitochondrial function in cardiac regeneration aims to enhance myocardial infarction and heart failure treatment methods and insights.PMID:38647881 | DOI:10.1007/s12265-024-10514-w (Source: Cell Research)
Source: Cell Research - April 22, 2024 Category: Cytology Authors: Yi-Xi Chen An-Ran Zhao Tian-Wen Wei Hao Wang Lian-Sheng Wang Source Type: research
Pirin Promotes the Progression of Non-Small-Cell Lung Cancer by Increasing ODC1 to Suppress Autophagy
J Proteome Res. 2024 Apr 22. doi: 10.1021/acs.jproteome.3c00871. Online ahead of print.ABSTRACTNon-small-cell lung cancer (NSCLC), a common malignant tumor, requires deeper pathogenesis investigation. Autophagy is an evolutionarily conserved lysosomal degradation process that is frequently blocked during cancer progression. It is an urgent need to determine the novel autophagy-associated regulators in NSCLC. Here, we found that pirin was upregulated in NSCLC, and its expression was positively correlated with poor prognosis. Overexpression of pirin inhibited autophagy and promoted NSCLC proliferation. We then performed data...
Source: Cell Research - April 22, 2024 Category: Cytology Authors: Yan-Ping Li Zi-Jia Huang Quan-Kuo He Yi-Xiang Li Xiang-Pei Zhao Zhong-Qi Ma Mei-Jing Qin Ai-Wen Chen Qiu Wei Yang Wang Chun-Hua Lu Source Type: research
Kinesin Facilitates Phenotypic Targeting of Therapeutic Resistance in Advanced Prostate Cancer
In this study, we use in vitro and in vivo preclinical MDA PCa PDX models of resistant human prostate cancer to determine molecular mechanisms of cross-resistance between anti-androgen therapy and taxane chemotherapy, underlying the therapeutically resistant phenotype. Transcriptomic profiling revealed that resistant and sensitive prostate cancer C4-2B cells have a unique differential gene signature response to cabazitaxel. Gene pathway analysis showed that sensitive cells exhibit increase in DNA damage, while resistant cells express genes associated with protein regulation in response to cabazitaxel. These PDX specimens a...
Source: Cell Research - April 22, 2024 Category: Cytology Authors: Maddison Archer Diane Begemann Edgar Gonzalez-Kozlova Prerna R Nepali Estefania Labanca Peter Shepherd Navneet Dogra Nora Navone Natasha Kyprianou Source Type: research
Nardilysin in vascular smooth muscle cells controls blood pressure via the regulation of calcium dynamics
In this study, we aimed to clarify the role of NRDC in vascular smooth muscle cell (VSMC) by generating VSMC-specific Nrdc knockout (VSMC-KO) mice. Our findings reveal that VSMC-KO mice also exhibit hypotension. Aortas isolated from VSMC-KO mice exhibited a weakened contractile response to phenylephrine, accompanied by reduced phosphorylation of myosin light chain 2 and decreased rhoA expression. VSMC isolated from VSMC-KO aortas showed a reduced increase in intracellular Ca2+ concentration induced by α-stimulants. These findings suggest that NRDC in VSMC regulates vascular contraction and blood pressure by modulating Ca2...
Source: Cell Research - April 22, 2024 Category: Cytology Authors: Mend Amar Batbaatar Takeshi Kinoshita Shinya Ikeda Kiyoto Nishi Hirotaka Iwasaki Narangerel Ganbaatar Mikiko Ohno Eiichiro Nishi Source Type: research
Changes in repair pathways of radiation-induced DNA double-strand breaks at the midblastula transition in Xenopus embryo
This study examines the impact of X-ray irradiation on Xenopus embryos before and after the MBT. The findings reveal a heightened X-ray sensitivity in embryos prior to the MBT, indicating a distinct shift in the DNA repair pathway during embryo development. Importantly, we show a transition in the dominant DSB repair pathway from NHEJ to HR before and after the MBT. These results suggest that the MBT plays a crucial role in altering DSB repair mechanisms, thereby influencing the IR sensitivity of developing embryos.PMID:38648785 | DOI:10.1093/jrr/rrae012 (Source: Cell Research)
Source: Cell Research - April 22, 2024 Category: Cytology Authors: Ryosuke Morozumi Naoto Shimizu Kouhei Tamura Makoto Nakamura Atsushi Suzuki Hiroko Ishiniwa Hiroshi Ide Masataka Tsuda Source Type: research
Chronic Sleep Deprivation Impairs Retinal Circadian Transcriptome and Visual Function
This study investigates these effects using a chronic sleep deprivation (CSD) model in mice. Our investigation reveals that CSD significantly alters the retinal circadian transcriptome, leading to remarkable changes in the temporal patterns of enriched pathways. This perturbation extends to metabolic and immune-related transcriptomes, coupled with an accumulation of reactive oxygen species in the retina. Notably, CSD rhythmically affects the thickness of the ganglion cell complex, along with diurnal shifts in microglial migration and morphology within the retina. Most critically, we observe a marked decrease in both scotop...
Source: Cell Research - April 22, 2024 Category: Cytology Authors: Shenzhen Huang Wenxiao Zhang Shuting Xuan Hongli Si Duliurui Huang Mengru Ba Di Qi Xiaoting Pei Dingli Lu Zhijie Li Source Type: research
Inhibition of Pim kinases triggers a broad antiviral activity by affecting innate immunity and via the PI3K-Akt-mTOR axis the endolysosomal system
Antiviral Res. 2024 Apr 20:105891. doi: 10.1016/j.antiviral.2024.105891. Online ahead of print.ABSTRACTZoonoses such as ZIKV and SARS-CoV-2 pose a severe risk to global health. There is urgent need for broad antiviral strategies based on host-targets filling gaps between pathogen emergence and availability of therapeutic or preventive strategies. Significant reduction of pathogen titers decreases spread of infections and thereby ensures health systems not being overloaded and public life to continue. Based on previously observed interference with FGFR1/2-signaling dependent impact on interferon stimulated genes (ISGs)-expr...
Source: Cell Research - April 22, 2024 Category: Cytology Authors: Mirco Glitscher Nuka Ivalu Benz Catarina Sabino Robin Murra Sascha Hein Tobias Zahn Ines Mhedhbi Debora Stefanova Daniela Bender Sabine Werner Eberhard Hildt Source Type: research
Dietary docosahexaenoic acid (DHA) downregulates liver DHA synthesis by inhibiting eicosapentaenoic acid elongation
In conclusion, we identify a novel feedback inhibition pathway where dietary DHA downregulates its liver synthesis by inhibiting EPA elongation.PMID:38649096 | DOI:10.1016/j.jlr.2024.100548 (Source: Cell Research)
Source: Cell Research - April 22, 2024 Category: Cytology Authors: Adam H Metherel Rodrigo Valenzuela Brinley J Klievik Giulia Cisbani Ruxandra D Rotarescu Melissa Gonzalez-Soto C éline Cruciani-Guglielmacci Sophie Lay é Christophe Magnan David M Mutch Richard P Bazinet Source Type: research
Evaluation puramatrix as a 3D microenvironment for neural differentiation of human breastmilk stem cells
In this study, we employed the PuraMatrix hydrogel scaffold as an advanced cell culture platform for the neural differentiation of stem cells derived from human breastmilk to design an opportune model for tissue engineering. Isolated stem cells from breastmilk were cultured and differentiated into neural-like cells on PuraMatrix peptide hydrogel and in the two-dimensional system. The compatibility of breastmilk-derived stem cells with PuraMatrix and cell viability was evaluated by scanning electron microscopy and MTT assay, respectively. Induction of differentiation was achieved by exposing cells to the neurogenic medium. ...
Source: Cell Research - April 22, 2024 Category: Cytology Authors: Nasim Goudarzi Ronak Shabani Fatemeh Moradi Marzieh Ebrahimi Majid Katebi Amir Jafari Shayesteh Mehdinejadiani Gelareh Vahabzade Mansoure Soleimani Source Type: research
