Regulation of cellular responses to X-ray irradiation through the activation of lysophosphatidic acid (LPA) receptor-3 (LPA < sub > 3 < /sub > ) and LPA < sub > 2 < /sub > in osteosarcoma cells
Pathol Res Pract. 2024 Apr 5;257:155293. doi: 10.1016/j.prp.2024.155293. Online ahead of print.ABSTRACTLysophosphatidic acid (LPA) binds to its specific G protein-coupled LPA receptors (LPA1 to LPA6), resulting in the activation of various cellular functions. LPA receptor-mediated signaling facilitates tumor progression in human malignancies. In the present study, we investigated whether LPA receptor-mediated signaling contributes to cellular responses to X-ray irradiation in osteosarcoma MG-63 cells. After X-ray irradiation (2, 4 and 8 Gy), LPAR2 and LPAR3 expression levels in MG-63 cells were significantly elevated in a ...
Source: Cell Research - April 14, 2024 Category: Cytology Authors: Hiroko Ikeda Miwa Takai Narumi Yashiro Yuka Amano Koki Hara Mao Yamamoto Toshifumi Tsujiuchi Source Type: research
Eliminating extracellular autoinducing peptide signals inhibits the Staphylococcus aureus quorum sensing agr system
This study evaluated the effectiveness of inhibiting agr QS wherein AIP trap carriers were made to coexist when culturing Staphylococcus aureus. Immersing a nitrocellulose (NC) membrane in Staphylococcus aureus ATCC 12600 culture inhibited QS-dependent α-hemolysin production, which significantly reduced the hemolysis ratio of sheep red blood cells by the culture supernatant. A quartz crystal microbalance analysis supported AIP adsorption onto the NC membrane. Adding the NC membrane during culture was found to maintain the expression levels of the agr QS gene agrA and α-hemolysin gene hla lower than that when it was not a...
Source: Cell Research - April 14, 2024 Category: Cytology Authors: Ruki Inagaki Ayaka Koshiba Eri Nasuno Norihiro Kato Source Type: research
Roles of HIF-1 α signaling in Mycobacterium tuberculosis infection: New targets for anti-TB therapeutics?
Biochem Biophys Res Commun. 2024 Apr 9;711:149920. doi: 10.1016/j.bbrc.2024.149920. Online ahead of print.ABSTRACTTuberculosis (TB), a deadly infectious disease induced by Mycobacterium tuberculosis (Mtb), continues to be a global public health issue that kill millions of patents every year. Despite significant efforts have been paid to identify effective TB treatments, the emergence of drug-resistant strains of the disease and the presence of comorbidities in TB patients urges us to explore the detailed mechanisms involved in TB immunity and develop more effective innovative anti-TB strategies. HIF-1α, a protein involved...
Source: Cell Research - April 14, 2024 Category: Cytology Authors: Chaowei Li Jiajun Wang Jun-Fa Xu Jiang Pi Biying Zheng Source Type: research
Generation of two induced pluripotent stem cell lines (LVPEIi007-B, LVPEIi008-B) from patients harboring homozygous mutation in ABCA4 (c.6088C > T) using non-integrative Sendai virus-based approach
Stem Cell Res. 2024 Apr 9;77:103418. doi: 10.1016/j.scr.2024.103418. Online ahead of print.ABSTRACTMutations in ABCA4 gene leads to the most common form of an inherited retinal disease namely, the Stargardt disease, type 1. Here, we report the generation of two different patient-specific induced pluripotent stem cell lines (LVPEIi007-B and LVPEIi008-B), carrying an identical homozygous mutation, (c.6088C>T) within the exon 44 of ABCA4 gene. These lines were generated by the reprogramming of patient-specific dermal fibroblasts, using the integration-free, Sendai viral vectors. Both lines were stably expanded and expresse...
Source: Cell Research - April 14, 2024 Category: Cytology Authors: Divya Pidishetty Savitri Maddileti Sudipta Mahato Trupti Agrawal Vinay Kumar Pulimamidi Milind Naik Chitra Kannabiran Subhadra Jalali Indumathi Mariappan Source Type: research
Generation of integration-free human induced pluripotent stem cells from a patient with sporadic Parkinson's disease
Stem Cell Res. 2024 Apr 8;77:103416. doi: 10.1016/j.scr.2024.103416. Online ahead of print.ABSTRACTA human induced pluripotent stem cell (iPSC) line (KKUi002-A) was generated from a skin fibroblast of a 57-years-old (at sampling) male patient diagnosed with a sporadic Parkinson's disease (PD). A non-integration system was used to reprogram fibroblasts into iPSCs by an episomal vector (OCT4/p53, SOX2/KLF4, L-MYC/LIN28). The KKUi002-A iPSCs displayed typical iPSC morphology, expressed pluripotency markers, differentiated into derivatives of three germ layers, and had a normal karyotype. These PD-derived iPSCs can be used to ...
Source: Cell Research - April 14, 2024 Category: Cytology Authors: Yukyeong Lee Dahee Jeong Seokbeom Ham Jaeseung Son Kinarm Ko Source Type: research
Metabolic Syndrome and Cardiac Vessel Remodeling Associated with Vessel Rarefaction: A Possible Underlying Mechanism May Result from a Poor Angiogenic Response to Altered VEGF Signaling Pathways
J Vasc Res. 2024 Apr 12:1-9. doi: 10.1159/000538361. Online ahead of print.ABSTRACTBACKGROUND: Elevated mortality rates in patients with metabolic syndrome (MetS) are partly due to adverse remodeling of multiple organs, which may lead to cardiovascular disease, nonalcoholic fatty liver disease, kidney failure, or other conditions. MetS symptoms, such as obesity, hypertension, hyperglycemia, dyslipidemia, associated with insulin and leptin resistance, are recognized as major cardiovascular risk factors that adversely affect the heart.SUMMARY: Pathological cardiac remodeling is accompanied by endothelial cell dysfunction whi...
Source: Cell Research - April 14, 2024 Category: Cytology Authors: Krzysztof Bartkowiak Mateusz Bartkowiak Ewa Jankowska-Steifer Anna Ratajska Marek Kujawa Olga Anio łek Justyna Niderla-Bieli ńska Source Type: research
Redox signaling in cell fate: Beyond damage
Biochim Biophys Acta Mol Cell Res. 2024 Apr 12:119722. doi: 10.1016/j.bbamcr.2024.119722. Online ahead of print.ABSTRACTThis review explores the nuanced role of reactive oxygen species (ROS) in cell fate, challenging the traditional view that equates ROS with cellular damage. Through significant technological advancements in detecting localized redox states and identifying oxidized cysteines, a paradigm shift has emerged: from ROS as merely damaging agents to crucial players in redox signaling. We delve into the intricacies of redox mechanisms, which, although confined, exert profound influences on cellular physiological r...
Source: Cell Research - April 14, 2024 Category: Cytology Authors: Felix Lamontagne Cynthia Paz Trejo Natalia Zamorano Cuervo Nathalie Grandvaux Source Type: research
Urolithin a inhibits breast cancer progression via activating TFEB-mediated mitophagy in tumor macrophages
CONCLUSION: Our study elucidated the anti-breast cancer effect of UA from the perspective of tumor-associated macrophages. Specifically, TFEB is a crucial downstream target in macrophages.PMID:38615740 | DOI:10.1016/j.jare.2024.04.010 (Source: Cell Research)
Source: Cell Research - April 14, 2024 Category: Cytology Authors: Bowen Zheng Yuying Wang Baian Zhou Fengyuan Qian Diya Liu Danrong Ye Xiqian Zhou Lin Fang Source Type: research
Tanshinone IIA destabilizes SLC7A11 by regulating PIAS4-mediated SUMOylation of SLC7A11 through KDM1A, and promotes ferroptosis in breast cancer
CONCLUSION: Tan IIA promoted ferroptosis and inhibited tumor growth and metastasis via suppressing KDM1A/PIAS4/SLC7A11 axis.PMID:38615741 | DOI:10.1016/j.jare.2024.04.009 (Source: Cell Research)
Source: Cell Research - April 14, 2024 Category: Cytology Authors: Na Luo KeJing Zhang Xin Li Yu Hu Lei Guo Source Type: research
Health risk assessment of heavy metal(loid)s in road dust via dermal exposure pathway from a low latitude plateau provincial capital city: The importance of toxicological verification
In this study, the road dust samples were harvested from a typical low-latitude plateau provincial capital city Kunming, Southwest China. The total concentration and dermal bioaccessibility of heavy metal(loid)s in road dust were determined, and their health risks and cytotoxicity on human skin keratinocytes were also assessed. The average concentrations of As (28.5 mg/kg), Cd (2.65 mg/kg), Mn (671 mg/kg), and Zn (511 mg/kg) exceeded the soil background values. Arsenic had the highest bioaccessibility after 2 h (3.79%), 8 h (4.24%), and 24 h (16.6%) extraction. Although soil assessment methods showed no health risk in the ...
Source: Cell Research - April 14, 2024 Category: Cytology Authors: Jingya Li Daolei Cui Ziyue Yang Jiaoyang Ma Jianjun Liu Yingxin Yu Xianfeng Huang Ping Xiang Source Type: research
Possible Retinotoxicity of Long-term Vardenafil Treatment
Exp Eye Res. 2024 Apr 12:109890. doi: 10.1016/j.exer.2024.109890. Online ahead of print.ABSTRACTPhosphodiesterase (PDE) inhibitors - such as vardenafil - are used primarily for treating erectile dysfunction via increasing cyclic guanosine monophosphate (cGMP) levels. Recent studies have also demonstrated their significant cardioprotective effects in several diseases, including diabetes, upon long-term, continuous application. However, PDE inhibitors are not specific for PDE5 and also inhibit the retinal isoform. A sustained rise in cGMP in photoreceptors is known to be toxic; therefore, we hypothesized that long-term varde...
Source: Cell Research - April 14, 2024 Category: Cytology Authors: Klaudia Szab ó Bulcs ú Dékány Anna Énzsöly Rozina Ida Hajd ú Lenke Korn élia Laurik-Feuerstein Arnold Szab ó Tam ás Radovits Csaba M átyás Attila Ol áh Kriszti án András Kovács Ágoston Szél G ábor Márk Somfai Ákos Lukáts Source Type: research
Metabolic Regulation of the Th17/Treg Balance in Inflammatory Bowel Disease
Pharmacol Res. 2024 Apr 12:107184. doi: 10.1016/j.phrs.2024.107184. Online ahead of print.ABSTRACTInflammatory bowel disease (IBD) is a long-lasting and inflammatory autoimmune condition affecting the gastrointestinal tract, impacting millions of individuals globally. The balance between T helper 17 (Th17) cells and regulatory T cells (Tregs) is pivotal in the pathogenesis and progression of IBD. This review summarizes the pivotal role of Th17/Treg equilibrium in maintaining intestinal homeostasis, elucidating how its dysregulation contributes to the development and exacerbation of IBD. It comprehensively synthesizes the c...
Source: Cell Research - April 14, 2024 Category: Cytology Authors: Shunfen Zhang Ruqing Zhong Shanlong Tang Liang Chen Hongfu Zhang Source Type: research
12,13-diHOME attenuates high glucose-induced calcification of vascular smooth muscle cells through repressing CPT1A-mediated HMGB1 succinylation
This study enrolled 159 elderly patients with type 2 diabetes and divided them into three groups, T1, T2 and T3, according to brachial-ankle pulse wave velocity(BaPWV). There were statistically significant differences in the waist circumference, waist hip ratio, systolic blood pressure, 12,13-diHOME (a lipokin) concentration among T1, T2 and T3. 12,13-diHOME levels were positively correlated to high density lipoprotein cholesterol and total cholesterol, but negatively correlated to with waist circumference, waist hip ratio, systolic blood pressure and baPWV. Studies in vitro showed that 12,13-diHOME effectively inhibits ca...
Source: Cell Research - April 14, 2024 Category: Cytology Authors: Huahua Li Lingling Zou Jin Zheng Tao Yang Source Type: research
Tissue-specific inflammation and insulin sensitivity in subjects with obesity
Diabetes Res Clin Pract. 2024 Apr 12:111663. doi: 10.1016/j.diabres.2024.111663. Online ahead of print.ABSTRACTObesity is associated with low-grade inflammation and insulin resistance (IR). The contribution of adipose tissue (AT) and hepatic inflammation to IR remains unclear. We conducted a study across three cohorts to investigate this relationship. The first cohort consists of six women with normal weight and twenty with obesity. In women with obesity, we found an upregulation of inflammatory markers in subcutaneous and visceral adipose tissue, isolated AT macrophages, and the liver, but no linear correlation with tissu...
Source: Cell Research - April 14, 2024 Category: Cytology Authors: S M Oussaada M Kilicarslan B A de Weijer P W Gilijamse A Şekercan S Virtue I M C Janssen A van de Laar A Demirkiran B A van Wagensveld A P J Houdijk A Jongejan P D Moerland J Verheij T B Geijtenbeek V W Bloks M C de Goffau J A Romijn M Nieuwdorp A Vidal- Source Type: research
Influence of a mesial cantilever on stress, strain, and axial force in fixed partial dentures with a distally tilted implant in the atrophic posterior maxilla
CONCLUSIONS: Mesial cantilever usage had minimal effect on stress or strain distribution in FPD implants, abutments, or prostheses. However, it helped resist screw loosening. The distal screw access hole was preferably positioned close to the prosthetic end.PMID:38616127 | DOI:10.2186/jpr.JPR_D_23_00218 (Source: Cell Research)
Source: Cell Research - April 14, 2024 Category: Cytology Authors: Xiaohui Fan Li Chen Qiuren Chen Feng Wang Yiqun Wu Yuanyuan Sun Source Type: research
