Basement Membrane Mimetic Hydrogel Cooperates with Rho-Associated Protein Kinase Inhibitor to Promote the Development of Acini-Like Salivary Gland Spheroids
Adv Nanobiomed Res. 2023 Nov;3(11):2300088. doi: 10.1002/anbr.202300088. Epub 2023 Oct 8.ABSTRACTSuccessful engineering of functional salivary glands necessitates the creation of cell-instructive environments for ex vivo expansion and lineage specification of primary human salivary gland stem cells (hS/PCs). Herein, basement membrane mimetic hydrogels were prepared using hyaluronic acid, cell adhesive peptides, and hyperbranched polyglycerol (HPG), with or without sulfate groups, to produce "hyperGel+" or "hyperGel", respectively. Differential scanning fluorescence experiments confirmed the ability of the sulphated HPG pre...
Source: Cell Research - April 22, 2024 Category: Cytology Authors: Eric W Fowler Robert L Witt Xinqiao Jia Source Type: research
Integrated Analysis Identified TGFBI as a Biomarker of Disease Severity and Prognosis Correlated with Immune Infiltrates in Patients with Sepsis
CONCLUSION: TGFBI warrants additional exploration as a promising biomarker for assessing illness severity and prognosis in patients with sepsis, considering its significant association with immunological and inflammatory responses in this condition.PMID:38645878 | PMC:PMC11027929 | DOI:10.2147/JIR.S456132 (Source: Cell Research)
Source: Cell Research - April 22, 2024 Category: Cytology Authors: Mingjie Shi Yue Wei Runmin Guo Fei Luo Source Type: research
Phosphoproteomic Analysis Identified Mutual Phosphorylation of FAK and Src as a Mechanism of Osimertinib Resistance in < em > EGFR < /em > -Mutant Lung Cancer
CONCLUSIONS: Phosphoproteomic analysis may help elucidate the mechanisms of resistance to molecular-targeted therapies in lung cancer. Mutual phosphorylation of FAK and Src is involved in osimertinib resistance. Thus, FAK and Src inhibition may be novel treatment strategies for osimertinib-resistant NSCLC.PMID:38646155 | PMC:PMC11031815 | DOI:10.1016/j.jtocrr.2024.100668 (Source: Cell Research)
Source: Cell Research - April 22, 2024 Category: Cytology Authors: Takehiro Tozuka Rintaro Noro Keisuke Yoshida Satoshi Takahashi Mariko Hirao Kuniko Matsuda Yasuhiro Kato Shinji Nakamichi Susumu Takeuchi Masaru Matsumoto Akihiko Miyanaga Shinobu Kunugi Kazufumi Honda Jun Adachi Masahiro Seike Source Type: research
Trem2 promotes foamy macrophage lipid uptake and survival in atherosclerosis
Nat Cardiovasc Res. 2023 Nov;2(11):1015-1031. doi: 10.1038/s44161-023-00354-3. Epub 2023 Oct 30.ABSTRACTAtherosclerosis is driven by the expansion of cholesterol-loaded 'foamy' macrophages in the arterial intima. Factors regulating foamy macrophage differentiation and survival in plaque remain poorly understood. Here we show, using trajectory analysis of integrated single-cell RNA sequencing data and a genome-wide CRISPR screen, that triggering receptor expressed on myeloid cells 2 (Trem2) is associated with foamy macrophage specification. Loss of Trem2 led to a reduced ability of foamy macrophages to take up oxidized low-...
Source: Cell Research - April 22, 2024 Category: Cytology Authors: Michael T Patterson Maria M Firulyova Yingzheng Xu Hannah Hillman Courtney Bishop Alisha Zhu Grant H Hickok Patricia R Schrank Christine E Ronayne Zakariya Caillot Gavin Fredrickson Ainsley E Kennedy Nisha Acharya Jaap G Neels Giulia Chinetti Xavier Revel Source Type: research
Inhibition of CDK7 mitigates doxorubicin cardiotoxicity and enhances anticancer efficacy
CONCLUSIONS: Activation of CDK7 is necessary for DOX-induced cardiomyocyte apoptosis and cardiomyopathy. Our findings uncover a novel proapoptotic role for CDK7 in cardiomyocytes. Moreover, this study suggests that inhibition of CDK7 attenuates DOX-induced cardiotoxicity, but augments the anticancer efficacy of DOX. Therefore, combined administration of CDK7 inhibitor and DOX may exhibit diminished cardiotoxicity but superior anticancer activity.PMID:38646672 | DOI:10.1093/cvr/cvae084 (Source: Cell Research)
Source: Cell Research - April 22, 2024 Category: Cytology Authors: Jingrui Chen Jing Wei Peng Xia Yuening Liu Mahder Dawit Belew Ryan Toohill Boyang Jason Wu Zhaokang Cheng Source Type: research
Aquaporins enriched in endothelial vacuole membrane regulate the diameters of microvasculature in hyperglycemia
CONCLUSION: EC-enriched aquaporins regulate the diameter of blood vessels through an intracellular vacuole-mediated process under hyperglycemic conditions. These findings collectively suggest that aquaporins expressed in ECs hold significant promise as potential targets for gene therapy aimed at addressing vascular perfusion defects associated with diabetes.PMID:38646676 | DOI:10.1093/cvr/cvae085 (Source: Cell Research)
Source: Cell Research - April 22, 2024 Category: Cytology Authors: Changsheng Chen Yinyin Qin Yidan Xu Xiaoning Wang Wei Lei Xiaozhong Shen Lixun Chen Linnong Wang Jie Gong Yongming Wang Shijun Hu Dong Liu Source Type: research
Urease and human glioblastoma (U87) cells growth inhibitory iridoid glycoside acetates from < em > Nyctanthes arbor-tristis < /em > Linn
Nat Prod Res. 2024 Apr 22:1-9. doi: 10.1080/14786419.2024.2340059. Online ahead of print.ABSTRACTThe present study was undertaken on the chemical constituents of ethanol extract of aerial parts of Nyctanthes arbor-tristis Linn., and their determination of growth inhibitory activity against glioblastoma multiforme cell line (U87) and urease inhibitory activity. Six constituents were isolated including two new arbortristoside F tetraacetate (1) and arbortristoside G heptaacetate (2) and four known arborside A tetraacetate (3), arborside C pentaacetate (4), 6,7-di-O-acetyl-6β-hydroxyloganin hexaacetate (5) and nyctanthoside ...
Source: Cell Research - April 22, 2024 Category: Cytology Authors: Talea Sana Sumbul Zehra Majid Khan Bina Shaheen Siddiqui Shabana Usman Simjee Sabira Begum Taibi B Hadda Source Type: research
A new benzoquinone and a new stilbenoid from < em > Paphiopedilum exul < /em > (Ridl.) Rolfe
Nat Prod Res. 2024 Apr 22:1-9. doi: 10.1080/14786419.2024.2344196. Online ahead of print.ABSTRACTOne new alkyl benzoquinone, paphionone (1), one new trans-stilbenoid, (E)-6,5'-dihydroxy-2,3'-dimethoxystilbene (2), and eight known stilbenoids and flavonoids (3-10) were isolated from the leaves and roots of Paphiopedilum exul (Orchidaceae). Their chemical structures were determined based on IR, ECD, MS and NMR analyses. Cytotoxicity of all isolated compounds towards human hepatocellular carcinoma (HepG2) cell line was examined in vitro by MTT assay. The para-hydroxybenzyl substituted stilbene 10 was potently cytotoxic to the...
Source: Cell Research - April 22, 2024 Category: Cytology Authors: Nonthalert Lertnitikul Ladawan Teerasukpimol Pattatorn Aekanantakul Naphatsawan Pooreecharurot Suchada Sukrong Cherdsak Boonyong Preeyaporn Poldorn Thanyada Rungrotmongkol Edwin Risky Sukandar Chanat Aonbangkhen Rutt Suttisri Source Type: research
Role of Mesenchymal Stem Cell-Derived Conditioned Medium in Modulating the Benzalkonium Chloride-Induced Cytotoxic Effects in Cultured Corneal Epithelial Cells In Vitro
CONCLUSIONS: Our results suggest that the MSC-CM is effective in modulating the BAK-induced cell death, retardation of metabolic activity and proliferation in cultured HCECs, particularly at lower concentration (0.001%) and shorter exposure (15 min) of BAK.PMID:38646923 | DOI:10.1080/02713683.2024.2342355 (Source: Cell Research)
Source: Cell Research - April 22, 2024 Category: Cytology Authors: Sreya Mitra Vasudeva Tati Sayan Basu Sachin Shukla Source Type: research
Rose Bengal Photodynamic Therapy (RB-PDT) Modulates the Inflammatory Response in LPS-Stimulated Human Corneal Fibroblasts By Influencing NF- κB and p38 MAPK Signaling Pathways
CONCLUSION: Rose bengal photodynamic therapy of lipopolysaccharide-stimulated human corneal fibroblasts can modify the inflammatory response by inducing interleukin-6 and interleukin-8 expression, and decreasing intercellular adhesion molecule-1 production. C-C motif chemokine ligand-4, interferon regulatory factor-3, and interferon α and β expressions are not affected by rose bengal photodynamic therapy in these cells. The underlying mechanisms may be associated with nuclear factor kappa B and p38 mitogen-activated protein kinase pathway activation.PMID:38646925 | DOI:10.1080/02713683.2024.2342600 (Source: Cell Research)
Source: Cell Research - April 22, 2024 Category: Cytology Authors: Ning Chai Tanja Stachon Tim Berger Zhen Li Maryam Amini Shweta Suiwal Berthold Seitz Achim Langenbucher N óra Szentmáry Source Type: research
Live-cell imaging reveals the trade-off between target search flexibility and efficiency for Cas9 and Cas12a
Nucleic Acids Res. 2024 Apr 22:gkae283. doi: 10.1093/nar/gkae283. Online ahead of print.ABSTRACTCRISPR-Cas systems have widely been adopted as genome editing tools, with two frequently employed Cas nucleases being SpyCas9 and LbCas12a. Although both nucleases use RNA guides to find and cleave target DNA sites, the two enzymes differ in terms of protospacer-adjacent motif (PAM) requirements, guide architecture and cleavage mechanism. In the last years, rational engineering led to the creation of PAM-relaxed variants SpRYCas9 and impLbCas12a to broaden the targetable DNA space. By employing their catalytically inactive varia...
Source: Cell Research - April 22, 2024 Category: Cytology Authors: Lorenzo Olivi Cleo Bagchus Victor Pool Ezra Bekkering Konstantin Speckner Hidde Offerhaus Wen Y Wu Martin Depken Koen J A Martens Raymond H J Staals Johannes Hohlbein Source Type: research
Extensive long-range polycomb interactions and weak compartmentalization are hallmarks of human neuronal 3D genome
Nucleic Acids Res. 2024 Apr 22:gkae271. doi: 10.1093/nar/gkae271. Online ahead of print.ABSTRACTChromatin architecture regulates gene expression and shapes cellular identity, particularly in neuronal cells. Specifically, polycomb group (PcG) proteins enable establishment and maintenance of neuronal cell type by reorganizing chromatin into repressive domains that limit the expression of fate-determining genes and sustain distinct gene expression patterns in neurons. Here, we map the 3D genome architecture in neuronal and non-neuronal cells isolated from the Wernicke's area of four human brains and comprehensively analyze ne...
Source: Cell Research - April 22, 2024 Category: Cytology Authors: Ilya A Pletenev Maria Bazarevich Diana R Zagirova Anna D Kononkova Alexander V Cherkasov Olga I Efimova Eugenia A Tiukacheva Kirill V Morozov Kirill A Ulianov Dmitriy Komkov Anna V Tvorogova Vera E Golimbet Nikolay V Kondratyev Sergey V Razin Philipp Khai Source Type: research
SifiNet: a robust and accurate method to identify feature gene sets and annotate cells
Nucleic Acids Res. 2024 Apr 22:gkae307. doi: 10.1093/nar/gkae307. Online ahead of print.ABSTRACTSifiNet is a robust and accurate computational pipeline for identifying distinct gene sets, extracting and annotating cellular subpopulations, and elucidating intrinsic relationships among these subpopulations. Uniquely, SifiNet bypasses the cell clustering stage, commonly integrated into other cellular annotation pipelines, thereby circumventing potential inaccuracies in clustering that may compromise subsequent analyses. Consequently, SifiNet has demonstrated superior performance in multiple experimental datasets compared with...
Source: Cell Research - April 22, 2024 Category: Cytology Authors: Qi Gao Zhicheng Ji Liuyang Wang Kouros Owzar Qi-Jing Li Cliburn Chan Jichun Xie Source Type: research
Comprehensive profiling of L1 retrotransposons in mouse
Nucleic Acids Res. 2024 Apr 22:gkae273. doi: 10.1093/nar/gkae273. Online ahead of print.ABSTRACTL1 elements are retrotransposons currently active in mammals. Although L1s are typically silenced in most normal tissues, elevated L1 expression is associated with a variety of conditions, including cancer, aging, infertility and neurological disease. These associations have raised interest in the mapping of human endogenous de novo L1 insertions, and a variety of methods have been developed for this purpose. Adapting these methods to mouse genomes would allow us to monitor endogenous in vivo L1 activity in controlled, experimen...
Source: Cell Research - April 22, 2024 Category: Cytology Authors: Xuanming Zhang Ivana Celic Hannah Mitchell Sam Stuckert Lalitha Vedula Jeffrey S Han Source Type: research
admetSAR3.0: a comprehensive platform for exploration, prediction and optimization of chemical ADMET properties
Nucleic Acids Res. 2024 Apr 22:gkae298. doi: 10.1093/nar/gkae298. Online ahead of print.ABSTRACTAbsorption, distribution, metabolism, excretion and toxicity (ADMET) properties play a crucial role in drug discovery and chemical safety assessment. Built on the achievements of admetSAR and its successor, admetSAR2.0, this paper introduced the new version of the series, admetSAR3.0, as a comprehensive platform for chemical ADMET assessment, including search, prediction and optimization modules. In the search module, admetSAR3.0 hosted over 370 000 high-quality experimental ADMET data for 104 652 unique compounds, and supplemen...
Source: Cell Research - April 22, 2024 Category: Cytology Authors: Yaxin Gu Zhuohang Yu Yimeng Wang Long Chen Chaofeng Lou Chen Yang Weihua Li Guixia Liu Yun Tang Source Type: research
