Changes in repair pathways of radiation-induced DNA double-strand breaks at the midblastula transition in Xenopus embryo

This study examines the impact of X-ray irradiation on Xenopus embryos before and after the MBT. The findings reveal a heightened X-ray sensitivity in embryos prior to the MBT, indicating a distinct shift in the DNA repair pathway during embryo development. Importantly, we show a transition in the dominant DSB repair pathway from NHEJ to HR before and after the MBT. These results suggest that the MBT plays a crucial role in altering DSB repair mechanisms, thereby influencing the IR sensitivity of developing embryos.PMID:38648785 | DOI:10.1093/jrr/rrae012
Source: Cell Research - Category: Cytology Authors: Source Type: research