Modification of a Selective NTRK2 Agonist and Confirmation of Activity in a Glaucoma-on-a-Chip Model
CONCLUSION: The modified peptide studied herein showed improved stability over the original peptide (RNYK) and demonstrated potential for use as a BDNF agonist with neuroprotective properties for treatment of neurodegenerative disorders such as glaucoma.PMID:38638624 | PMC:PMC11022028 | DOI:10.18502/jovr.v19i1.15439 (Source: Cell Research)
Source: Cell Research - April 19, 2024 Category: Cytology Authors: Fatemeh Nafian Shahin Yazdani Mohammad Javad Rasaee Babak Kamali Doust Azad Narsis Daftarian Mozhgan Rezaei Kanavi Source Type: research
Polymeric Propranolol Nanoparticles for Intraocular Delivery: Formulation, Characterization, and Vitreous Pharmacokinetics
CONCLUSION: PLGA NPs can prolong the existence of propranolol in both vitreous and posterior ocular tissues, and thus, may provide an effective approach in treatment of posterior segment neovascularization.PMID:38638633 | PMC:PMC11022021 | DOI:10.18502/jovr.v19i1.15436 (Source: Cell Research)
Source: Cell Research - April 19, 2024 Category: Cytology Authors: Farkhondeh Chaharband Reyhaneh Varshochian Rassoul Dinarvand Hamideh Sabbaghi Mozhgan Rezaei Kanavi Narsis Daftarian Ramin Nourinia Source Type: research
PsmiR159b-PsMYB65 module functions in the resumption of bud growth after endodormancy by affecting the cell cycle in tree peony
Hortic Res. 2024 Feb 23;11(4):uhae052. doi: 10.1093/hr/uhae052. eCollection 2024 Apr.ABSTRACTBud endodormancy in perennial plants is a sophisticated system that adapts to seasonal climatic changes. Growth-promoting signals such as low temperature and gibberellins (GAs) are crucial for facilitating budbreak following endodormancy release (EDR). However, the regulatory mechanisms underlying GA-mediated budbreak in tree peony (Paeonia suffruticosa) remain unclear. In tree peony, the expression of PsmiR159b among three differentially expressed miR159 members was inhibited with the prolonged chilling, and overexpression of PsMI...
Source: Cell Research - April 19, 2024 Category: Cytology Authors: Tao Zhang Xinyu Wang Yanchao Yuan Shoujie Zhu Chunying Liu Yuxi Zhang Shupeng Gai Source Type: research
Single-Cell Gene-Regulatory Networks of Advanced Symptomatic Atherosclerosis
CONCLUSIONS: By identifying and integrating the most gene-rich single-cell subclusters of atherosclerosis to date with a coronary artery disease framework of GRNs, GRN39 was identified and independently validated as being critical for the transformation of contractile SMCs into an osteogenic phenotype promoting advanced-stage, symptomatic atherosclerosis.PMID:38639096 | DOI:10.1161/CIRCRESAHA.123.323184 (Source: Cell Research)
Source: Cell Research - April 19, 2024 Category: Cytology Authors: Giuseppe Mocci Katyayani Sukhavasi Tiit Örd Sean Bankier Prosanta Singha Uma Thanigai Arasu Olayinka Oluwasegun Agbabiaje Petri M äkinen Lijiang Ma Chani J Hodonsky Redouane Aherrahrou Lars Muhl Jianping Liu Sonja Gustafsson Byambajav Byandelger Ying Wa Source Type: research
GLI1+ Cells Contribute to Vascular Remodeling in Pulmonary Hypertension
CONCLUSIONS: Our data highlight GLI1+ cells as an alternative cellular source of VSMCs in pulmonary hypertension and suggest that these cells and the associated signaling pathways represent an important therapeutic target for further studies.PMID:38639105 | DOI:10.1161/CIRCRESAHA.123.323736 (Source: Cell Research)
Source: Cell Research - April 19, 2024 Category: Cytology Authors: Xuran Chu Vahid Kheirollahi Arun Lingampally Prakash Chelladurai Chanil Valasarajan Ana Ivonne Vazquez-Armendariz Stefan Hadzic Ali Khadim Oleg Pak Stefano Rivetti Jochen Wilhelm Marek Bartkuhn Slaven Crnkovic Alena Moiseenko Monika Heiner Simone Kraut Le Source Type: research
Bisphenol-F and Bisphenol-S (BPF and BPS) Impair the Stemness of Neural Stem Cells and Neuronal Fate Decision in the Hippocampus Leading to Cognitive Dysfunctions
Mol Neurobiol. 2024 Apr 18. doi: 10.1007/s12035-024-04160-1. Online ahead of print.ABSTRACTNeurogenesis occurs throughout life in the hippocampus of the brain, and many environmental toxicants inhibit neural stem cell (NSC) function and neuronal generation. Bisphenol-A (BPA), an endocrine disrupter used for surface coating of plastic products causes injury in the developing and adult brain; thus, many countries have banned its usage in plastic consumer products. BPA analogs/alternatives such as bisphenol-F (BPF) and bisphenol-S (BPS) may also cause neurotoxicity; however, their effects on neurogenesis are still not known. ...
Source: Cell Research - April 18, 2024 Category: Cytology Authors: Saurabh Tiwari None Phoolmala Shweta Goyal Ranjeet Kumar Yadav Rajnish Kumar Chaturvedi Source Type: research
Performance of NiO doped on alkaline sludge from waste photovoltaic industries for catalytic dry reforming of methane
Environ Sci Pollut Res Int. 2024 Apr 18. doi: 10.1007/s11356-024-33325-7. Online ahead of print.ABSTRACTAlkali sludge (AS) is waste abundantly generated from solar photovoltaic (PV) solar cell industries. Since this potential basic material is still underutilized, a combination with NiO catalyst might greatly influence coke resentence, especially in high-temperature thermochemical reactions (Arora and Prasad, RSC Adv. 6:108,668-108688, 2016). This paper investigated alkaline sludge containing 3CaO-2SiO2 doped with well-known NiO to enhance the dry reforming of methane (DRM) reaction. The wet-impregnation method was used to...
Source: Cell Research - April 18, 2024 Category: Cytology Authors: Mohd Razali Shamsuddin Siow Hwa Teo Tengku Sharifah Marliza Tengku Azmi Azizul Hakim Lahuri Yun Hin Taufiq-Yap Source Type: research
Correlation between erythrocyte parameters and iron status in cyclic Spanish Purebred mares
Vet Res Commun. 2024 Apr 18. doi: 10.1007/s11259-024-10376-2. Online ahead of print.ABSTRACTThe aim of this study was to evaluate the serum iron status and its relation to hematological indexes in cyclic mares. Blood samples were taken from 40 Spanish Purebred mares on days - 5, 0, + 5 and + 16 of their cycle. Concentration of transferrin (TRF) was significantly lower on day 0 than on days + 5 and + 16, transferrin saturation (TSAT) decreased significantly on days 0 and + 16 compared to day - 5, total iron-binding capacity (TIBC) on day + 16 was significantly higher than those on days - 5 and 0, and on day + 5 it was also ...
Source: Cell Research - April 18, 2024 Category: Cytology Authors: Katiuska Satu é Esterina Fazio Elena Dami á Pietro Medica Cristina Cravana Source Type: research
Arginine ingestion inhibits phagocyte invasion in eccentrically contracted rat fast-twitch muscle
This study aimed to investigate whether prior ingestion of L-arginine (ARG), a nitric oxide precursor, inhibits ECC-induced macrophage invasion. Male Wistar rats received ARG in water for 7 days, beginning 3 days prior to ECC. ECCs were induced in the anterior crural muscles for 200 cycles. Three days later, the tibialis anterior and extensor digitorum longus muscles were excised for biochemical analysis and force measurement, respectively. ARG ingestion increased nitrite and nitrate levels in plasma and muscle, inhibiting force depression and reducing CD68 content in muscles subjected to ECC. ARG ingestion also ameliorate...
Source: Cell Research - April 18, 2024 Category: Cytology Authors: Keita Kanzaki Masanobu Wada Source Type: research
Validation of a novel western blot assay to monitor patterns and levels of alpha dystroglycan in skeletal muscle of patients with limb girdle muscular dystrophies
J Muscle Res Cell Motil. 2024 Apr 18. doi: 10.1007/s10974-024-09670-y. Online ahead of print.ABSTRACTThe cell membrane protein, dystroglycan, plays a crucial role in connecting the cytoskeleton of a variety of mammalian cells to the extracellular matrix. The α-subunit of dystroglycan (αDG) is characterized by a high level of glycosylation, including a unique O-mannosyl matriglycan. This specific glycosylation is essential for binding of αDG to extracellular matrix ligands effectively. A subset of muscular dystrophies, called dystroglycanopathies, are associated with aberrant, dysfunctional glycosylation of αDG. This de...
Source: Cell Research - April 18, 2024 Category: Cytology Authors: Thulashitha Rajasingham Hector M Rodriguez Andreas Betz Douglas M Sproule Uma Sinha Source Type: research
Coadministration of fluconazole to boost subtherapeutic sirolimus concentrations: A case report
Pharmacol Res Perspect. 2024 Jun;12(3):e1198. doi: 10.1002/prp2.1198.ABSTRACTIndividual sirolimus whole blood concentrations are highly variable, critically influenced by the concomitant use of cytochrome P450 (CYP) 3A inducers or inhibitors, and also modulated by food. Therapeutic drug monitoring is therefore recommended, especially at treatment start or in circumstances that can influence sirolimus exposure. In this case report, we highlight the challenge of achieving therapeutic sirolimus concentrations and present pragmatic solutions with regimen adaptions, pharmacokinetic enhancement (use of a drug-drug interaction), ...
Source: Cell Research - April 18, 2024 Category: Cytology Authors: Camilo Scherkl Andreas D Meid Sven E Cuntz Laura Classen Johanna Weiss David Czock Walter E Haefeli Source Type: research
Hemisynthesis and cytotoxic evaluation of manoyl oxide analogs from sclareol: effect of two tertiary hydroxyls & amp; Heck coupling on cytotoxicity
Nat Prod Res. 2024 Apr 18:1-10. doi: 10.1080/14786419.2024.2342558. Online ahead of print.ABSTRACTSclareol, a bioactive diterpene alcohol isolated from Salvia sclarea, was subjected to structural modification and cytotoxic evaluation. Boron-Heck-coupled analogs of manoyl oxide were prepared from sclareol in a two-step reaction scheme. In the first step manoyl oxide was prepared from sclareol using cerium (IV) ammonium nitrate. Further the structural modification of manoyl oxide via Palladium (II) catalysed Boron-Heck coupling reaction produced a new series of compounds. All the synthesised compounds were screened for in vi...
Source: Cell Research - April 18, 2024 Category: Cytology Authors: Ifshana Gani Zahidul Islam Sofi Gursimar Kaur Anindya Goswami Khursheed Ahmad Bhat Source Type: research
Saikosaponin a promotes neutrophil extracellular trap formation and bactericidal activity
This study aimed to investigate the effects of SSa, one of the major triterpenoid saponins extracted from Radix bupleuri, on neutrophil extracellular trap (NET) formation and the mechanism associated with this process. Using Sytox green and immunofluorescence assays, we found SSa rapidly induced NET formation, which depended on NADPH oxidase (NOX)-independent ROS production and autophagy. Pharmacologic inhibitor studies indicated that ERK and PI3K/AKT signalling were also required for SSa-induced NET formation, whereas protein arginine deiminase 4 (PAD4) was not required. Furthermore, we found that SSa promoted neutrophil ...
Source: Cell Research - April 18, 2024 Category: Cytology Authors: Lanqiu Zhang Zhengwei Hu Lei Yang Tianyu Liu Jing Xun Qi Zhang Ximo Wang Hejun Gao Zhongkui Jin Source Type: research
Deuterium metabolic imaging differentiates glioblastoma metabolic subtypes and detects early response to chemoradiotherapy
Cancer Res. 2024 Apr 18. doi: 10.1158/0008-5472.CAN-23-2552. Online ahead of print.ABSTRACTMetabolic subtypes of glioblastoma have different prognoses and responses to treatment. Deuterium metabolic imaging with 2H-labeled substrates is a potential approach to stratify patients into metabolic subtypes for targeted treatment. Here, we used 2H magnetic resonance spectroscopy (MRS) and spectroscopic imaging (MRSI) measurements of [6,6'-2H2]glucose metabolism to identify metabolic subtypes and their responses to chemoradiotherapy in patient-derived glioblastoma xenografts in vivo. The metabolism of patient-derived cells was fi...
Source: Cell Research - April 18, 2024 Category: Cytology Authors: Jacob Chen Ming Low Jianbo Cao Friederike Hesse Alan J Wright Anastasia Tsyben Islam Alshamleh Richard Mair Kevin M Brindle Source Type: research
Omental preadipocytes stimulate matrix remodeling and IGF signaling to support ovarian cancer metastasis
Cancer Res. 2024 Apr 18. doi: 10.1158/0008-5472.CAN-23-2613. Online ahead of print.ABSTRACTOvarian cancer can metastasize to the omentum, which is associated with a complex tumor microenvironment. Omental stromal cells facilitate ovarian cancer colonization by secreting cytokines and growth factors. Improved understanding of the tumor supportive functions of specific cell populations in the omentum could identify strategies to prevent and treat ovarian cancer metastasis. Here, we showed that omental preadipocytes enhance the tumor initiation capacity of ovarian cancer cells. Secreted factors from preadipocytes supported ca...
Source: Cell Research - April 18, 2024 Category: Cytology Authors: Jennifer A Waters Mikella Robinson Omar Lujano-Olazaba Cassidy Lucht Samuel F Gilbert Carrie D House Source Type: research
