Analysis of the Cadmium Removal Mechanism of Human Gut Bacteria Enterococcus faecalis Strain ATCC19433 from a Genomic Perspective
This study evaluated the Cd removal capability and mechanism of Enterococcus faecalis strain ATCC19433 while annotating the genetic functions related to Cd removal, which may facilitate the development of potential human gut strains for the removal of Cd.PMID:38602649 | DOI:10.1007/s12011-024-04169-6 (Source: Cell Research)
Source: Cell Research - April 11, 2024 Category: Cytology Authors: Yu Zheng Zhibin Duan Yonggui Wu Yang Luo Xiaoyu Peng Jianye Wu Source Type: research
Acesulfame potassium upregulates PD-L1 in HCC cells by attenuating autophagic degradation
Biochem Biophys Res Commun. 2024 Apr 9;711:149921. doi: 10.1016/j.bbrc.2024.149921. Online ahead of print.ABSTRACTArtificial sweeteners, which contain no or few calories, have been widely used in various foods and beverages, and are regarded as safe alternatives to sugar by the Food and Drug Administration. While several studies suggest that artificial sweeteners are not related to cancer development, some research has reported their potential association with the risk of cancers, including hepatocellular carcinoma (HCC). Here, we investigated whether acesulfame potassium (Ace K), a commonly used artificial sweetener, indu...
Source: Cell Research - April 11, 2024 Category: Cytology Authors: Dong-Ho Kim Eun-Jun Kwon Keun-Gyu Park Jonghwa Jin Jun-Kyu Byun Source Type: research
Synergistic effects of graphene microgrooves and electrical stimulation on M2 macrophage polarization
In this study, we prepared a graphene free-standing substrate with 20 μm microgrooves using capillary forces induced by water evaporation. Subsequently, we established an ES cell culture platform for macrophage cultivation by integrating a self-designed multi-well chamber cell culture device. We observed that graphene microgrooves, in combination with ES, significantly reduce cell spreading area and circularity. Results from immunofluorescence, ELISA, and flow cytometry demonstrate that the synergistic effect of graphene microgrooves and ES effectively promotes macrophage M2 phenotypic polarization. Finally, RNA sequencin...
Source: Cell Research - April 11, 2024 Category: Cytology Authors: Yan Yan Yilin Zhang Kexin Li Yudong Li Wei Qian Wenbo Zhang Yue Wang Wensheng Ma Linhao Li Source Type: research
Identification and validation of anoikis-related genes to clarify the prognosis and immune mechanisms of patients with low-grade glioma
CONCLUSIONS: This study confirmed that TIMP1 promoted the malignant progression of LGG by inhibiting anoikis, providing insights into LGG pathogenesis and potential therapeutic targets.PMID:38603834 | DOI:10.1016/j.bbrc.2024.149894 (Source: Cell Research)
Source: Cell Research - April 11, 2024 Category: Cytology Authors: Ji Wang Yantin Liu Min Qian Wen Xu Jianfeng Chen Yuanguo Deng Ran Luo Jian Chen Wenxue Jia Longhu Liu Chunlei Tian Source Type: research
Neutrophil extracellular traps induced by IL-1 β promote endothelial dysfunction and aggravate limb ischemia
This study investigated the functional significance of NETs in vascular inflammation and remodeling associated with limb ischemia. Single-cell RNA sequencing (scRNA-seq) and flow cytometry revealed neutrophil activation and upregulated NETs formation in human limb ischemia, with immunofluorescence confirming IL-1β-induced release of NETs for vascular inflammation. Endothelial cell activation was examined via scRNA-seq and western blotting, indicating enhanced proliferation, expression of adhesion molecules (VCAM-1, ICAM-1), inflammatory cytokines (IL-1β, IL-6) and decreased expression of VE-cadherin, that could be mediat...
Source: Cell Research - April 11, 2024 Category: Cytology Authors: Shigang Lin Pengwei Zhu Liujun Jiang Yujian Hu Lirui Huang Yangyan He Hongkun Zhang Source Type: research
Anaerobic co-digestion of microalgal biomass, sugarcane vinasse, and residual glycerol from biodiesel using simplex-centroid mixture design: methane potential, synergic effect, and microbial diversity
Environ Sci Pollut Res Int. 2024 Apr 12. doi: 10.1007/s11356-024-33193-1. Online ahead of print.ABSTRACTMicroalgal biomass (MB) is a promising feedstock for bioenergy production. Nonetheless, the cell recalcitrance and the low C/N ratio limit the methane yield during anaerobic digestion. As an alternative to overcome these challenges, MB co-digestion with different feedstocks has been proposed. Thus, this study evaluated the anaerobic co-digestion (AcoD) of MB cultivated in wastewater with sugarcane vinasse (VIN) and residual glycerol from biodiesel production (GLY). Batch tests were conducted using augmented simplex-centr...
Source: Cell Research - April 11, 2024 Category: Cytology Authors: Edilberto Mariano da Silva Sayonara Costa de Ara újo Shyrlane Torres Soares Veras Agnes Adam Duarte Pinheiro Fabr ício Motteran Mario Takayuki Kato Lourdinha Florencio Wanderli Rog ério Moreira Leite Source Type: research
Chitin-mediated blockade of chitinase-like proteins reduces tumor immunosuppression, inhibits lymphatic metastasis and enhances anti-PD-1 efficacy in complementary TNBC models
CONCLUSIONS: Chitin, as a general CLP blocker, reduces CLP production, enhances anti-tumor immunity as well as ICB responses, supporting its potential clinical relevance in immunosuppressed TNBC patients.PMID:38605414 | DOI:10.1186/s13058-024-01815-8 (Source: Cell Research)
Source: Cell Research - April 11, 2024 Category: Cytology Authors: Robbe Salembier Caro De Haes Julie Bellemans Kristel Demeyere Wim Van Den Broeck Niek N Sanders Steven Van Laere Traci R Lyons Evelyne Meyer Jonas Steenbrugge Source Type: research
HERC5 downregulation in non-small cell lung cancer is associated with altered energy metabolism and metastasis
CONCLUSIONS: Taken together, these results indicate that low HERC5 expression increases the metastatic potential of NSCLC in vitro and in vivo. Furthermore, HERC5-induced proteomic changes influence mitochondrial pathways, ultimately leading to alterations in energy metabolism and demonstrating its role as a new potential metastasis suppressor gene.PMID:38605423 | DOI:10.1186/s13046-024-03020-z (Source: Cell Research)
Source: Cell Research - April 11, 2024 Category: Cytology Authors: Svenja Schneegans Jana L öptien Angelika Mojzisch Desir ée Loreth Oliver Kretz Christoph Raschdorf Annkathrin Hanssen Antonia Gocke Bente Siebels Karthikeyan Gunasekaran Yi Ding Leticia Oliveira-Ferrer Laura Brylka Thorsten Schinke Hartmut Schl üter Il Source Type: research
LncRNAs: Emerging biomarkers and therapeutic targets in rectal cancer
Pathol Res Pract. 2024 Apr 4;257:155294. doi: 10.1016/j.prp.2024.155294. Online ahead of print.ABSTRACTAccording to findings, long non-coding RNAs (lncRNAs) have an important function in the onset and growth of various cancers, including rectal cancer (RC). RC offers unique issues in terms of diagnosis, treatment, and results, needing a full understanding of the cellular mechanisms that cause it to develop. This thorough study digs into the various functions that lncRNAs perform in RC, giving views into their multiple roles as well as possible therapeutic consequences. The function of lncRNAs in RC cell proliferation, apop...
Source: Cell Research - April 11, 2024 Category: Cytology Authors: None Abida Mohd Imran Lina Eltaib Akbar Ali Razan Abdulaziz Salem Alanazi Neelam Singla Syed Mohammed Basheeruddin Asdaq Marwan Al-Hajeili Fatemah Abdulaziz Alhakami Saleh Al-Abdulhadi Altaf A Abdulkhaliq Ali A Rabaan Source Type: research
Low-molecular-weight fucoidan increases telomere length and immunostimulatory effects on NK-92 cells following inhaled anesthetic injury
In this study, we determined the effect of isoflurane on telomeres and examined the potential of LMF to ameliorate the harmful effects of isoflurane. Male Lewis rats, the mouse lymphoma cell line YAC-1, and the human nature killer cell line NK-92 MI were exposed to isoflurane. The relative telomere length (T/S) ratio and mRNA expression were determined by quantitative PCR. The viability assay was used to assess cell viability. In vivo, 2% isoflurane exposure, which is a clinically relevant concentration, reduced telomere length, and correlated with exposure frequency and duration. Isoflurane concentrations above 2% shorten...
Source: Cell Research - April 11, 2024 Category: Cytology Authors: Cheng-Hsi Chang Pai-An Hwang Source Type: research
Cytotoxicity of emerging halophenylacetamide disinfection byproducts in drinking water: Mechanism and prediction
This study provided the first comprehensive investigation on the cytotoxicity mechanisms of HPAcAm DBPs.PMID:38604064 | DOI:10.1016/j.watres.2024.121562 (Source: Cell Research)
Source: Cell Research - April 11, 2024 Category: Cytology Authors: Shaoyang Hu Xiangxiang Li Falin He Yuntao Qi Beibei Zhang Rutao Liu Source Type: research
The effect of Schizophyllan on the differentiation of osteoclasts and osteoblasts
In this study, its effects on bone cells, particularly osteoclasts and osteoblasts, were examined. We demonstrated that SPG dose-dependently inhibited osteoclastogenesis and reduced gene expression associated with osteoclast differentiation. SPG also decreased bone resorption and F-actin ring formation. This inhibition could have been due to the downregulation of transcription factors c-Fos and nuclear factor of activated T cells 1 (NFATc1) via the MAPKs (JNK and p38), IκBα, and PGC1β/PPARγ pathways. In coculture, SPG lowered osteoclastogenic activity in calvaria-derived osteoblasts by reducing macrophage colony-stimul...
Source: Cell Research - April 11, 2024 Category: Cytology Authors: Soo-Ho Kim Keun Ha Park Jun Lee Seoung Hoon Lee Jeong-Hwa Baek Source Type: research
Exosomal Tenascin-C Primes Macrophage Pyroptosis Amplifying Aberrant Inflammation during Sepsis-Induced Acute Lung Injury
Transl Res. 2024 Apr 9:S1931-5244(24)00078-1. doi: 10.1016/j.trsl.2024.04.001. Online ahead of print.ABSTRACTSepsis-induced acute lung injury (ALI) is a serious complication of sepsis and the predominant cause of death. Exosomes released by lung tissue cells critically influence the progression of ALI during sepsis by modulating the inflammatory microenvironment. However, the molecular mechanisms by which exosome-mediated intercellular signaling exacerbates ALI in septic infection remain undefined. Our study found increased levels of exosomal Tenascin-C (TNC) in the plasma of both patients and mice with ALI, showing a stro...
Source: Cell Research - April 11, 2024 Category: Cytology Authors: Ting Gong Xuedi Zhang Xiaolei Liu Yinfeng Ye Zhiyuan Tian Shuang Yin Min Zhang Jing Tang Youtan Liu Source Type: research
Proteomic and metabolomic insights into seed germination of Ferula assa-foetida
J Proteomics. 2024 Apr 9:105176. doi: 10.1016/j.jprot.2024.105176. Online ahead of print.ABSTRACTCold stratification is known to affect the speed of seed germination; however, its regulation at the molecular level in Ferula assa-foetida remains ambiguous. Here, we used cold stratification (4 °C in the dark) to induce germination in Ferula assa-foetida and adopted a proteomic and metabolomic approach to understand the molecular mechanism of germination. Compared to the control, we identified 209 non-redundant proteins and 96 metabolites in germinated F. assa-foetida seed. Results highlight the common and unique regulatory ...
Source: Cell Research - April 11, 2024 Category: Cytology Authors: Ashwani Punia Manglesh Kumari Monika Chouhan Vishal Saini Robin Joshi Ashok Kumar Rajiv Kumar Source Type: research
Isobavachalcone alleviates ischemic stroke by suppressing HDAC1 expression and improving M2 polarization
In this study, we evaluated the influence of ISO in both transient middle cerebral artery occlusion/reperfusion (tMCAO/R) rat models and oxygen-glucose deprivation/reperfusion (OGD/R) cell models. We observed that pretreatment with 50mg/kg ISO diminished the volume of brain infarction, reduced brain edema, and ameliorated neurological deficits in rats. A reduction in Nissl bodies was noted in the tMCAO/R group, which was reversed following treatment with 50mg/kg ISO. TUNEL/NeuN double staining revealed a decrease in TUNEL-positive cells in tMCAO/R rats treated with ISO. Furthermore, ISO treatment suppressed the expression ...
Source: Cell Research - April 11, 2024 Category: Cytology Authors: Qiannan Zhang Junting Dai Yongzhong Lin L I Miao Source Type: research
