A mutation in the cardiac KV7.1 channel possibly disrupts interaction with Yotiao protein
Biochem Biophys Res Commun. 2024 Apr 16;714:149947. doi: 10.1016/j.bbrc.2024.149947. Online ahead of print.ABSTRACTHere, we characterized the p.Arg583His (R583H) Kv7.1 mutation, identified in two unrelated families suffered from LQT syndrome. This mutation is located in the HС-HD linker of the cytoplasmic portion of the Kv7.1 channel. This linker, together with HD helix are responsible for binding the A-kinase anchoring protein 9 (AKAP9), Yotiao. We studied the electrophysiological characteristics of the mutated channel expressed in CHO-K1 along with KCNE1 subunit and Yotiao protein, using the whole-cell patch-clamp techn...
Source: Cell Research - April 24, 2024 Category: Cytology Authors: Bowen Li Maria Karlova Han Zhang Oksana B Pustovit Lisha Mai Valery Novoseletsky Dmitry Podolyak Elena V Zaklyazminskaya Denis V Abramochkin Olga S Sokolova Source Type: research
Crystal structures of human CD40 in complex with monoclonal antibodies dacetuzumab and bleselumab
Biochem Biophys Res Commun. 2024 Apr 18;714:149969. doi: 10.1016/j.bbrc.2024.149969. Online ahead of print.ABSTRACTCD40 is a member of the tumor necrosis factor receptor superfamily, and it is widely expressed on immune and non-immune cell types. The interaction between CD40 and the CD40 ligand (CD40L) plays an essential function in signaling, and the CD40/CD40L complex works as an immune checkpoint molecule. CD40 has become a therapeutic target, and a variety of agonistic/antagonistic anti-CD40 monoclonal antibodies (mAbs) have been developed. To better understand the mode of action of anti-CD40 mAbs, we determined the X-...
Source: Cell Research - April 24, 2024 Category: Cytology Authors: Risa Asano Makoto Nakakido Jorge Fern ández Pérez Tomoko Ise Jose M M Caaveiro Satoshi Nagata Kouhei Tsumoto Source Type: research
Transforming growth factor-beta signals promote progression of squamous cell carcinoma by inducing epithelial-mesenchymal transition and angiogenesis
In this study, we examined the effects of TGF-β signaling on the murine squamous cell carcinoma, SCCVII cells in vitro and in vivo. The incubation of SCCVII cells with TGF-β induced the activation of TGF-β signals and epithelial-mesenchymal transition (EMT). Notably, the motility of SCCVII cells was increased upon the activation of the TGF-β signaling. RNA sequencing revealed upregulation of genes related to EMT and angiogenesis. Consistent with these in vitro results, the inhibition of TGF-β signals in SCCVII cell-derived primary tumors resulted in suppressed angiogenesis. Furthermore, we identified six candidate fac...
Source: Cell Research - April 24, 2024 Category: Cytology Authors: Haruka Ibi Kazuki Takahashi Hiroyuki Harada Tetsuro Watabe Katarzyna A Podyma-Inoue Source Type: research
Curcumin-loaded liposomes modulating the synergistic role of EpCAM and estrogen receptor alpha in lung cancer management
This study delves into the in vitro potential of liposomal therapeutics utilizing Curcumin-loaded PlexoZome® (CUR-PLXZ) in targeting EpCAM/TROP1 and Estrogen Receptor Alpha (ERα) signalling pathways for LC management. The prevalence of LC, particularly non-small cell lung cancer (NSCLC), underscores the urgent need for effective treatments. Biomarkers like EpCAM/TROP1 and ERα/NR3A1 play crucial roles in guiding targeted therapies and influencing prognosis. EpCAM plays a key role in cell-cell adhesion and signalling along with ERα which is a nuclear receptor that binds estrogen and regulates gene expression in response ...
Source: Cell Research - April 24, 2024 Category: Cytology Authors: Manisha Singh Gabriele De Rubis Sofia Kokkinis Keshav Raj Paudel Stewart Yeung Philip Michael Hansbro Brian Gregory George Oliver Kamal Dua Source Type: research
Clinical characteristics, proteins, and genes related to interstitial pneumonia-associated squamous cell carcinoma of the lungs
Pathol Res Pract. 2024 Apr 7;257:155292. doi: 10.1016/j.prp.2024.155292. Online ahead of print.ABSTRACTSquamous cell carcinoma (SCC) is a common histological type of lung carcinoma that is associated with interstitial pneumonia (IP). We hypothesized that identifying specific genetic alterations or molecular markers of SCC with IP may aid the development of novel therapeutic strategies for the same. Therefore, in the present study, we aimed to identify tumorigenic genetic alterations and molecular markers in cases of SCC with IP. We included 28 lung SCC cases (14 cases with IP and 14 cases without IP). We performed immunohi...
Source: Cell Research - April 24, 2024 Category: Cytology Authors: Akiko Mochizuki Haruto Nishida Ryo Kaimori Yoshihiko Kondo Hiroko Kadowaki Takahiro Kusaba Kazuhiro Kawamura Atsushi Osoegawa Kenji Sugio Tsutomu Daa Source Type: research
Exosomal long non-coding RNA AU020206 alleviates macrophage pyroptosis in atherosclerosis by suppressing CEBPB-mediated NLRP3 transcription
In conclusion, this study demonstrates that AU020206 delivered by BMSC-EXO alleviates macrophage pyroptosis in AS by blocking CEBPB-mediated transcriptional activation of NLRP3.PMID:38657723 | DOI:10.1016/j.yexcr.2024.114054 (Source: Cell Research)
Source: Cell Research - April 24, 2024 Category: Cytology Authors: Nan Zhang Yuxin Luo Jiawei Shao Huanhuan Sun Kai Ma Xiang Gao Source Type: research
Reconstruction with antibiotic loaded single-side gore-tex "Tartine" methyl-methacrylate cementoplasty for pediatric chest wall reconstruction: a 10-case series
DISCUSSION: "Tartine" cementoplasty is a simple, low-cost technique for pediatric chest wall reconstruction. It is well tolerated and checks key demands for chest wall reconstructions.LEVEL OF EVIDENCE: IV; retrospective case series.PMID:38657749 | DOI:10.1016/j.otsr.2024.103895 (Source: Cell Research)
Source: Cell Research - April 24, 2024 Category: Cytology Authors: Coline Ducrot Max Piffoux Pierre Yves Rabattu Anne Pourquet Quoc Peyrot Yohann Robert Franck Chotel Fr éderic Hameury Source Type: research
Targeting inflammasomes and pyroptosis in retinal diseases-molecular mechanisms and future perspectives
Prog Retin Eye Res. 2024 Apr 22:101263. doi: 10.1016/j.preteyeres.2024.101263. Online ahead of print.ABSTRACTRetinal diseases encompass various conditions associated with sight-threatening immune responses and are leading causes of blindness worldwide. These diseases include age-related macular degeneration, diabetic retinopathy, glaucoma and uveitis. Emerging evidence underscores the vital role of the innate immune response in retinal diseases, beyond the previously emphasized T-cell-driven processes of the adaptive immune system. In particular, pyroptosis, a newly discovered programmed cell death process involving inflam...
Source: Cell Research - April 24, 2024 Category: Cytology Authors: Yimeng Sun Fan Li Yunfei Liu Dijie Qiao Xinyu Yao Guei-Sheung Liu Dequan Li Chuanle Xiao Tao Wang Wei Chi Source Type: research
Discovery of baloxavir sodium as a novel anti-CCHFV inhibitor: biological evaluation of in vitro and in vivo
This study identified baloxavir sodium as a novel scaffold structure and mechanism of anti-CCHF compound, providing a promising new strategy for clinical treatment of CCHF after further optimization.PMID:38657838 | DOI:10.1016/j.antiviral.2024.105890 (Source: Cell Research)
Source: Cell Research - April 24, 2024 Category: Cytology Authors: Kai Liu Liushuai Li Yajie Liu Xi Wang Jia Liu Jiang Li Fei Deng Runze Zhang Yiwu Zhou Zhihong Hu Wu Zhong Manli Wang Chun Guo Source Type: research
Vitamin C and inhibition of the fibroblast growth factor pathway synergistically enhance myogenesis
Biochim Biophys Acta Mol Cell Res. 2024 Apr 22:119737. doi: 10.1016/j.bbamcr.2024.119737. Online ahead of print.NO ABSTRACTPMID:38657910 | DOI:10.1016/j.bbamcr.2024.119737 (Source: Cell Research)
Source: Cell Research - April 24, 2024 Category: Cytology Authors: Xiajie Fu Masayuki Funaba Source Type: research
The role of ion homeostasis in adaptation and tolerance to acetic acid stress in yeasts
FEMS Yeast Res. 2024 Apr 24:foae016. doi: 10.1093/femsyr/foae016. Online ahead of print.ABSTRACTMaintenance of asymmetric ion concentrations across cellular membranes is crucial for proper yeast cellular function. Disruptions of these ionic gradients can significantly impact membrane electrochemical potential and the balance of other ions, particularly under stressful conditions such as exposure to acetic acid. This weak acid, ubiquitous to both yeast metabolism and industrial processes, is a major inhibitor of yeast cell growth in industrial settings and a key determinant of host colonization by pathogenic yeast. Acetic a...
Source: Cell Research - April 24, 2024 Category: Cytology Authors: Miguel Antunes Isabel S á-Correia Source Type: research
Structural insights into human exon-defined spliceosome prior to activation
Cell Res. 2024 Apr 24. doi: 10.1038/s41422-024-00949-w. Online ahead of print.ABSTRACTSpliceosome is often assembled across an exon and undergoes rearrangement to span a neighboring intron. Most states of the intron-defined spliceosome have been structurally characterized. However, the structure of a fully assembled exon-defined spliceosome remains at large. During spliceosome assembly, the pre-catalytic state (B complex) is converted from its precursor (pre-B complex). Here we report atomic structures of the exon-defined human spliceosome in four sequential states: mature pre-B, late pre-B, early B, and mature B. In the p...
Source: Cell Research - April 24, 2024 Category: Cytology Authors: Wenyu Zhang Xiaofeng Zhang Xiechao Zhan Rui Bai Jianlin Lei Chuangye Yan Yigong Shi Source Type: research
Hirschsprung's disease: m6A methylase VIRMA suppresses cell migration and proliferation by regulating GSK3beta
CONCLUSION: VIRMA can inhibit cell migration and proliferation by upregulating the expression of GSK3β, contributing to the onset of HSCR.IMPACT: The expressions of VIRMA were significantly reduced in HSCR, while GSK3β expression was increased in HSCR, and can be used as a molecular marker. VIRMA overexpression promoted the proliferation and migration of SH-SY5Y and HEK-293T cells. VIRMA can inhibit the degradation of GSK3β mRNA and increase the expression of GSK3β.PMID:38658662 | DOI:10.1038/s41390-024-03136-0 (Source: Cell Research)
Source: Cell Research - April 24, 2024 Category: Cytology Authors: Yang Yang Mengzhen Zhang Nan Li Chen Wang Huirong Yang Xinwei Hou Jiaming Yang Kaisi Fan Liucheng Yang Kai Wu Source Type: research
GFRA4 improves the neurogenic potential of enteric neural crest stem cells via hedgehog pathway
CONCLUSION: GFRA4 promotes proliferation and differentiation but inhibits apoptosis of ENCSCs via the hedgehog pathway in HSCR.IMPACT: This study confirms that GFRA4 improves the proliferation and differentiation of ENCSCs via modulation of the hedgehog pathway. This study for the first time revealed the role and the mechanism of the action of GFRA4 in HSCR, which indicates that GFRA4 may play a role in the pathological development of HSCR. Our findings may lay the foundation for further investigation of the mechanisms underlying HSCR development and into targets of HSCR treatment.PMID:38658664 | DOI:10.1038/s41390-024-031...
Source: Cell Research - April 24, 2024 Category: Cytology Authors: Fangfang Zhang Mingyu Cui Lijuan Zhang Bangzhen Ma Feng Guo Gang Wang Source Type: research
CircPDE5A-encoded novel regulator of the PI3K/AKT pathway inhibits esophageal squamous cell carcinoma progression by promoting USP14-mediated de-ubiquitination of PIK3IP1
CONCLUSION: The novel protein PDE5A-500aa encoded by circPDE5A can act as an inhibitor of the PI3K/AKT signaling pathway to inhibit the progression of ESCC by promoting USP14-mediated de-ubiquitination of PIK3IP1 and may serve as a potential target for the development of therapeutic agents.PMID:38658954 | DOI:10.1186/s13046-024-03054-3 (Source: Cell Research)
Source: Cell Research - April 24, 2024 Category: Cytology Authors: Kai Lei Ruihao Liang Jialu Liang Nan Lu Jing Huang Ke Xu Binghua Tan Kexi Wang Yicheng Liang Wenjian Wang Huayue Lin Minghui Wang Source Type: research
