Integrative analysis of single-cell and bulk transcriptome data reveal the significant role of macrophages in lupus nephritis
CONCLUSION: Our results present an important pathophysiological role for macrophages in LN, and our preliminary results demonstrate significant pro-inflammatory effects of LGALS9 gene in LN macrophages.PMID:38610007 | DOI:10.1186/s13075-024-03311-y (Source: Cell Research)
Source: Cell Research - April 12, 2024 Category: Cytology Authors: Shuping Wei Haiyun Shen Yidan Zhang Chunrui Liu Shoushan Li Jing Yao Zhibin Jin Hongliang Yu Source Type: research
Circ-10720 as a ceRNA adsorbs microRNA-1238 and modulates ZEB2 to boost NSCLC development by activating EMT
CONCLUSIONS: Circ-10720 acts as a ceRNA to adsorb miR-1238 and modulate ZEB2 to facilitate the proliferation, migration, invasion, and EMT of NSCLC cells.PMID:38610009 | DOI:10.1186/s40001-024-01715-9 (Source: Cell Research)
Source: Cell Research - April 12, 2024 Category: Cytology Authors: Wei Zhang Ping Xiao Bin Liu Yan Zhang Source Type: research
Augmented ERO1 α upon mTORC1 activation induces ferroptosis resistance and tumor progression via upregulation of SLC7A11
CONCLUSIONS: The ERO1α/IL-6/STAT3/SLC7A11 pathway is crucial for mTORC1-mediated ferroptosis resistance and tumor growth, and combining ERO1α inhibition with ferroptosis inducers is a novel and effective treatment for mTORC1-related tumors.PMID:38610018 | DOI:10.1186/s13046-024-03039-2 (Source: Cell Research)
Source: Cell Research - April 12, 2024 Category: Cytology Authors: Zixi Wang Huaiyuan Zong Weiwei Liu Wei Lin Anjiang Sun Zhao Ding Xu Chen Xiaofeng Wan Yanyan Liu Zhongdong Hu Hongbing Zhang Hongwu Li Yehai Liu Dapeng Li Sumei Zhang Xiaojun Zha Source Type: research
Adipose-derived stem cells can alleviate RHDV2 induced acute liver injury in rabbits
Res Vet Sci. 2024 Apr 2;172:105255. doi: 10.1016/j.rvsc.2024.105255. Online ahead of print.ABSTRACTRabbit hemorrhagic disease virus (RHDV) can cause fatal fulminant hepatitis, which is very similar to human acute liver failure. The aim of this study was to investigate whether adipose-derived stem cells (ADSCs) could alleviate RHDV2-induced liver injury in rabbits. Twenty 50-day-old rabbits were divided randomly into two groups (RHDV2 group, ADSCs + RHDV2 group). Starting from the 1st day, two groups of rabbits were given 0.5 ml of viral suspensions by subcutaneous injection in the neck. Meanwhile, the ADSCs + RHDV2 group w...
Source: Cell Research - April 12, 2024 Category: Cytology Authors: Lihui Shi Yumei Liu Qianni Liu Chenhao Chang Weiqi Liu Ziqiang Zhang Source Type: research
Generation and characterization of the iPS cell line (SYSUSHi001-A) derived from the peripheral blood mononuclear cells (PBMCs) of a 33-year-old patient with acute myeloid leukemia (AML)
Stem Cell Res. 2024 Feb 23;77:103360. doi: 10.1016/j.scr.2024.103360. Online ahead of print.ABSTRACTWe successfully developed an induced pluripotent stem cell (iPSC) line, SYSUSHi001-A, from the peripheral blood mononuclear cells (PBMC) of a patient with Acute Myeloid Leukemia, harboring two genetic mutations (XPO1: c.591-4_591-3dupTT; PALB2: c.3296C > T; p.T1099M). This iPSC line was facilitated through the use of episomal plasmids encoding OCT4, SOX2, KLF4, L-MYC, and human miR-302. The SYSUSHi001-A iPSC line exhibited characteristic embryonic stem cell-like morphology, maintained the XPO1 and PALB2 mutations, express...
Source: Cell Research - April 12, 2024 Category: Cytology Authors: Lihua Yuan Mei Xie Yuan Tao Xi Chen Xiaojun Xu Xiaobo Wang Source Type: research
Targeting notch-related lncRNAs in cancer: Insights into molecular regulation and therapeutic potential
Pathol Res Pract. 2024 Apr 1;257:155282. doi: 10.1016/j.prp.2024.155282. Online ahead of print.ABSTRACTCancer is a group of diseases marked by unchecked cell proliferation and the ability for the disease to metastasize to different body areas. Enhancements in treatment and early detection are crucial for improved outcomes. LncRNAs are RNA molecules that encode proteins and have a length of more than 200 nucleotides. LncRNAs are crucial for chromatin architecture, gene regulation, and other cellular activities that impact both normal growth & pathological processes, even though they are unable to code for proteins. LncR...
Source: Cell Research - April 12, 2024 Category: Cytology Authors: Raihan Siddique Gaurav Gupta Johar Mgm Ashwani Kumar Harpreet Kaur I A Ariffin Atreyi Pramanik Waleed Hassan Almalki Haider Ali Moyad Shahwan Neeraj Patel Krishna Murari Riya Mishra Riya Thapa Asif Ahmad Bhat Source Type: research
Circulating immune cell phenotyping is potentially relevant for diabetic retinopathy risk assessment
CONCLUSION: We have demonstrated a strong correlation between immune cell traits and DR using a genetic approach. This finding offers valuable insights for early DR prevention and future clinical research and treatment.PMID:38609020 | DOI:10.1016/j.diabres.2024.111667 (Source: Cell Research)
Source: Cell Research - April 12, 2024 Category: Cytology Authors: Bo Li Xu Zhao Zhenzhen Hong Yan Ding Yi Zhang Source Type: research
Freezing pre-treatment improves radio frequency explosion puffing (RFEP) quality by altering the cellular structure of purple sweet potato [Ipomoea batatas (L) Lam.]
In conclusion, freezing pre-treatment before RFEP can significantly enhance the puffing quality, making this an effective method for preparing oil-free puffing products for fruits and vegetables.PMID:38609243 | DOI:10.1016/j.foodres.2024.114265 (Source: Cell Research)
Source: Cell Research - April 12, 2024 Category: Cytology Authors: Chao Mao Yurui Chen Tong Liu Pengfei Ye Yequn Wang Xiangwei Chen Hongfei Fu Yunyang Wang Ke Wang Source Type: research
Optimizing multi-domain hematologic biomarkers and clinical features for the differential diagnosis of unipolar depression and bipolar depression
Npj Ment Health Res. 2023 Apr 10;2(1):4. doi: 10.1038/s44184-023-00024-z.ABSTRACTThere is a lack of objective features for the differential diagnosis of unipolar and bipolar depression, especially those that are readily available in practical settings. We investigated whether clinical features of disease course, biomarkers from complete blood count, and blood biochemical markers could accurately classify unipolar and bipolar depression using machine learning methods. This retrospective study included 1160 eligible patients (918 with unipolar depression and 242 with bipolar depression). Patient data were randomly split into...
Source: Cell Research - April 12, 2024 Category: Cytology Authors: Jinkun Zeng Yaoyun Zhang Yutao Xiang Sugai Liang Chuang Xue Junhang Zhang Ya Ran Minne Cao Fei Huang Songfang Huang Wei Deng Tao Li Source Type: research
Functional analysis and validation of oncodrive gene AP3S1 in ovarian cancer through filtering of mutation data from whole-exome sequencing
CONCLUSION: This comprehensive analysis of somatic mutations in HGSOC provides insight into potential therapeutic targets and molecular pathways for targeted interventions. AP3S1 was identified as being a key player in tumor immunity and prognosis, thus providing new perspectives for personalized treatment strategies. The findings of this study contribute to the understanding of HGSOC pathogenesis and provide a foundation for improved outcomes in patients with this aggressive disease.PMID:38609993 | DOI:10.1186/s40001-024-01814-7 (Source: Cell Research)
Source: Cell Research - April 12, 2024 Category: Cytology Authors: Deshui Kong Yu Wu Qiyu Liu Cuiyu Huang Tongxia Wang Zongyao Huang Yan Gao Yuan Li Hongyan Guo Source Type: research
Metabolomics assisted by transcriptomics analysis to reveal metabolic characteristics and potential biomarkers associated with treatment response of neoadjuvant therapy with TCbHP regimen in HER2 + breast cancer
CONCLUSION: Serum-metabolomics could be used as a powerful tool for exploring informative biomarkers for predicting or monitoring treatment efficacy. Metabolomics integrated with transcriptomics analysis could assist in obtaining new insights into biochemical pathophysiology and might facilitate the development of new treatment targets for insensitive patients.PMID:38610016 | DOI:10.1186/s13058-024-01813-w (Source: Cell Research)
Source: Cell Research - April 12, 2024 Category: Cytology Authors: Ningning Zhang Yuxin Huang Guanwen Wang Yimei Xiang Zhouhong Jing Junjie Zeng Feng Yu Xianjun Pan Wenqi Zhou Xiaohua Zeng Source Type: research
The soluble (pro)renin receptor promotes a preeclampsia-like phenotype both in vitro and in vivo
Hypertens Res. 2024 Apr 11. doi: 10.1038/s41440-024-01678-8. Online ahead of print.ABSTRACTPreeclampsia is classified as new-onset hypertension coupled with gross endothelial dysfunction. Placental (pro)renin receptor ((P)RR) and plasma soluble (P)RR (s(P)RR) are elevated in patients with preeclampsia. Thus, we aimed to interrogate the role (P)RR may play in the pathogenesis of preeclampsia. Human uterine microvascular endothelial cells (HUtMECs, n = 4) were cultured with either; vehicle (PBS), 25-100 nM recombinant s(P)RR, or 10 ng/ml TNF-a (positive control) for 24 h. Conditioned media and cells were assessed for endothe...
Source: Cell Research - April 11, 2024 Category: Cytology Authors: Lachlan G Schofield Sarah J Delforce Jennifer C Pryor Saije K Endacott Eugenie R Lumbers Sarah A Marshall Kirsty G Pringle Source Type: research
Neutrophil extracellular traps induced by IL-1 β promote endothelial dysfunction and aggravate limb ischemia
This study investigated the functional significance of NETs in vascular inflammation and remodeling associated with limb ischemia. Single-cell RNA sequencing (scRNA-seq) and flow cytometry revealed neutrophil activation and upregulated NETs formation in human limb ischemia, with immunofluorescence confirming IL-1β-induced release of NETs for vascular inflammation. Endothelial cell activation was examined via scRNA-seq and western blotting, indicating enhanced proliferation, expression of adhesion molecules (VCAM-1, ICAM-1), inflammatory cytokines (IL-1β, IL-6) and decreased expression of VE-cadherin, that could be mediat...
Source: Cell Research - April 11, 2024 Category: Cytology Authors: Shigang Lin Pengwei Zhu Liujun Jiang Yujian Hu Lirui Huang Yangyan He Hongkun Zhang Source Type: research
Structural basis for human Ca < sub > v < /sub > 3.2 inhibition by selective antagonists
Cell Res. 2024 Apr 11. doi: 10.1038/s41422-024-00959-8. Online ahead of print.ABSTRACTThe Cav3.2 subtype of T-type calcium channels has been targeted for developing analgesics and anti-epileptics for its role in pain and epilepsy. Here we present the cryo-EM structures of Cav3.2 alone and in complex with four T-type calcium channel selective antagonists with overall resolutions ranging from 2.8 Å to 3.2 Å. The four compounds display two binding poses. ACT-709478 and TTA-A2 both place their cyclopropylphenyl-containing ends in the central cavity to directly obstruct ion flow, meanwhile extending their polar tails into the...
Source: Cell Research - April 11, 2024 Category: Cytology Authors: Jian Huang Xiao Fan Xueqin Jin Chen Lyu Qinmeng Guo Tao Liu Jiaofeng Chen Ama ël Davakan Philippe Lory Nieng Yan Source Type: research
System-level time computation and representation in the suprachiasmatic nucleus revealed by large-scale calcium imaging and machine learning
Cell Res. 2024 Apr 11. doi: 10.1038/s41422-024-00956-x. Online ahead of print.ABSTRACTThe suprachiasmatic nucleus (SCN) is the mammalian central circadian pacemaker with heterogeneous neurons acting in concert while each neuron harbors a self-sustained molecular clockwork. Nevertheless, how system-level SCN signals encode time of the day remains enigmatic. Here we show that population-level Ca2+ signals predict hourly time, via a group decision-making mechanism coupled with a spatially modular time feature representation in the SCN. Specifically, we developed a high-speed dual-view two-photon microscope for volumetric Ca2+...
Source: Cell Research - April 11, 2024 Category: Cytology Authors: Zichen Wang Jing Yu Muyue Zhai Zehua Wang Kaiwen Sheng Yu Zhu Tianyu Wang Mianzhi Liu Lu Wang Miao Yan Jue Zhang Ying Xu Xianhua Wang Lei Ma Wei Hu Heping Cheng Source Type: research
