DNM3OS/miR-127-5p/CDH11, activates Wnt3a/ β-catenin/LEF-1 pathway to form a positive feedback and aggravate spine facet joint osteoarthritis
In this study, spine facet joint tissue of patients were collected. In vitro and in vivo models were constructed with SW1353 cells and rats. Hematoxylin and eosin (HE) staining, Safranin O-fast Green, Alcian blue staining, and Tolueine blue O (TBO) staining were employed for histology analyses. Quantitative PCR, western blotting, and Immunofluorescence were performed to evaluate the expression of genes. The levels of inflammatory cytokines were measured by enzyme-linked immunosorbent assay analysis. Cell Counting Kit-8 and flow cytometry were used for cell activity and apoptosis evaluation. The targeting sites between micr...
Source: Cell Research - March 20, 2024 Category: Cytology Authors: Jing Wang Zhenyu Yang Xiuming He Yeyang Wang Dixin Luo Wangyang Xu Hongtao Zhang Xiaozhong Zhou Source Type: research

AKAP12 Upregulation Associates With PDE8A to Accelerate Cardiac Dysfunction
CONCLUSIONS: AKAP12 upregulation in cardiac tissue is associated with accelerated cardiac dysfunction through the AKAP12-PDE8 axis.PMID:38506047 | DOI:10.1161/CIRCRESAHA.123.323655 (Source: Cell Research)
Source: Cell Research - March 20, 2024 Category: Cytology Authors: Hanan Qasim Mehrdad Rajaei Ying Xu Arfaxad Reyes-Alcaraz Hala Y Abdelnasser M David Stewart Satadru K Lahiri Xander H T Wehrens Bradley K McConnell Source Type: research

Economic value of toripalimab plus axitinib as first-line treatment for advanced renal cell carcinoma in China: a model-based cost-effectiveness analysis
CONCLUSIONS: Toripalimab plus axitinib was unlikely to be the cost-effective first-line therapy for patients with previously untreated advanced RCC compared with sunitinib from the Chinese health-care system perspective.PMID:38506058 | DOI:10.1080/14737167.2024.2333334 (Source: Cell Research)
Source: Cell Research - March 20, 2024 Category: Cytology Authors: Shuo Kang Jintuo Yin Source Type: research

Role of mitochondrial dysfunction in the pathogenesis of rheumatoid arthritis: Looking closely at fibroblast- like synoviocytes
Mitochondrion. 2023 Nov;73:62-71. doi: 10.1016/j.mito.2023.10.004. Epub 2023 Oct 30.ABSTRACTRheumatoid arthritis (RA) is a chronic, autoimmune, and inflammatory disease that primarily targets the joints, leading to cartilage and bone destruction.Fibroblast-like synoviocytes (FLS) are specialized cells of the synovial lining in the joint that plays a fundamental role in the development of RA. Particularly, FLS of RA patients (RA-FLS) in the joint exhibit specific characteristics like higher invading and immunogenic properties, hyperproliferation, and reduced apoptotic capacity, suggesting a dysfunctional mitochondrial pool ...
Source: Cell Research - March 20, 2024 Category: Cytology Authors: Lakra Promila Anubha Joshi Shazia Khan Amita Aggarwal Amit Lahiri Source Type: research

Ephrin B1 Regulates Inflammatory Pathways in Retinal M üller Cells
In conclusion, a reduction in ephrin B1 in the diabetic retina may offer a new therapeutic modality for diabetic retinopathy.PMID:38501146 | PMC:PMC10947218 | DOI:10.33696/diabetes.6.056 (Source: Cell Research)
Source: Cell Research - March 19, 2024 Category: Cytology Authors: Li Liu Youde Jiang Mohamed Al-Shabrawey Jena J Steinle Source Type: research

The long-term effect of periodontitis treatment on changes in blood inflammatory markers in patients with generalized aggressive periodontitis
CONCLUSION: This study indicated that periodontal therapy may have beneficial effects on peripheral venous blood inflammatory markers in patients with GAgP during long-term observation.PMID:38501229 | DOI:10.1111/jre.13251 (Source: Cell Research)
Source: Cell Research - March 19, 2024 Category: Cytology Authors: Xiancheng Zeng Xiane Wang Xiaoyuan Guan Xianghui Feng Ruifang Lu Huanxin Meng Source Type: research

Periodontal tissue regeneration with cementogenesis after application of brain-derived neurotrophic factor in 3-wall inflamed intra-bony defect
CONCLUSION: These findings suggest that BDNF could induce cementum regeneration in early regenerative phase by stimulating proliferation of periodontal ligament cells and differentiation into periodontal tissue cells, resulting in enhancement of periodontal tissue regeneration in inflamed 3-wall intra-bony defects.PMID:38501357 | DOI:10.1111/jre.13244 (Source: Cell Research)
Source: Cell Research - March 19, 2024 Category: Cytology Authors: Mari Kiyota Tomoyuki Iwata Naohiko Hasegawa Shinya Sasaki Yuri Taniguchi Yuta Hamamoto Shinji Matsuda Kazuhisa Ouhara Katsuhiro Takeda Tsuyoshi Fujita Hidemi Kurihara Hiroyuki Kawaguchi Noriyoshi Mizuno Source Type: research

Mechanical properties of human kidney cells and their effects on the atomic force microscope beam vibrations
In conclusion, the FEM and experimental results were compared and found to be in good agreement. HIGHLIGHTS: Using Cisplatin anti-cancer drug increases the elastic modulus of ACHN cell. Applying plasma treatment increases the elastic modulus of ACHN cell. For both of the chemo and plasma therapies, increasing the incubation time increases the influence of therapies oh the cell mechanics. Using finite element modeling (FEM) the real dynamic behavior of atomic force microscope cantilever by considering human kidney cells as the soft samples is possible.PMID:38501545 | DOI:10.1002/jemt.24543 (Source: Cell Research)
Source: Cell Research - March 19, 2024 Category: Cytology Authors: Ali Jafari Ali Sadeghi Mansoureh Lafouti Source Type: research

A new labdane diterpenoid, < em > in vitro < /em > and < em > in silico < /em > cytotoxicity, and protease inhibitory effects of phytochemicals from < em > Juniperus polycarpos < /em > K. Koch leaves
Nat Prod Res. 2024 Mar 19:1-12. doi: 10.1080/14786419.2024.2323542. Online ahead of print.ABSTRACTCytotoxicity-guided purification of Juniperus polycarpos K. Koch leaves (Cupressaceae) led to the isolation of a new labdane diterpenoid, 3-(acetyloxy)-acetylisocupressic acid (1), together with isocupressic acid (2), 3,4-dimethoxycinnamoyl alcohol (3) and deoxypodophyllotoxin (4). The chemical structures of 1-4 were established by detailed 1D and 2D NMR, HRFAB-MS and LRESI-MS, as well as by comparing the spectral data with those reported in the literature. Compound 1 was ineffective against HepG2 cells and protease enzyme, wh...
Source: Cell Research - March 19, 2024 Category: Cytology Authors: Moslem Naderian Salar Hafez Ghoran Muhammad Ikhlas Abdjan Zahra Sabahi Soheila Moein Amir Reza Jassbi Mahmoodreza Moein Source Type: research

Study on chitosan/gelatin hydrogels containing ceria nanoparticles for promoting the healing of diabetic wound
J Biomed Mater Res A. 2024 Mar 19. doi: 10.1002/jbm.a.37701. Online ahead of print.ABSTRACTChronic inflammation at diabetic wound sites results in the uncontrolled accumulation of pro-inflammatory factors and reactive oxygen species (ROS), which impedes cell proliferation and delays wound healing. To promote the healing of diabetic wounds, chitosan/gelatin hydrogels containing ceria nanoparticles (CNPs) of various sizes were created in the current study. CNPs' efficacy in removing O 2 • - $$ {\mathrm{O}}_2^{\bullet -} $$ , •OH, and H2 O2 was demonstrated, and the scavenging ability of CNPs of varying sizes was com...
Source: Cell Research - March 19, 2024 Category: Cytology Authors: Yonghui Wu Qianqian Wu Xialian Fan Lu Yang Ling Zou Qingshan Liu Guangyou Shi Xiaochao Yang Keyong Tang Source Type: research

Two new megastigmane glycosides from the leaves of < em > Nicotiana tabacum < /em > and their anti-inflammatory activities
Nat Prod Res. 2024 Mar 19:1-5. doi: 10.1080/14786419.2024.2331042. Online ahead of print.ABSTRACTTwo new megastigmane glycosides, (6 R,7E,9R)-3-oxo-α-ionyl-9-O-α-L-rhamnopyranosyl-(1''→4')-β-D-glucopyranoside (1) and (6 R,7E,9R)-3-oxo-α-ionyl-9-O-β-D-glucopyranosyl-(1''→6')-β-D-glucopyranoside (2), together with six known analogues (3-8) were isolated from the leaves of Nicotiana tabacum. The structures of all metabolites were determined by comprehensive analysis of NMR and MS spectroscopic data as well as by comparison with those of previously reported. The in vitro anti-inflammatory activity of all isolates was...
Source: Cell Research - March 19, 2024 Category: Cytology Authors: Peng-Fei Yang Wei Wu Hang-Hang Han Qing-Fu Wang Lei Li Ze-Ning Wang Duo-Bin Mao Zhi-Fei Chen Source Type: research

Automated segmentation of cell organelles in volume electron microscopy using deep learning
Microsc Res Tech. 2024 Mar 19. doi: 10.1002/jemt.24548. Online ahead of print.ABSTRACTRecent advances in computing power triggered the use of artificial intelligence in image analysis in life sciences. To train these algorithms, a large enough set of certified labeled data is required. The trained neural network is then capable of producing accurate instance segmentation results that will then need to be re-assembled into the original dataset: the entire process requires substantial expertise and time to achieve quantifiable results. To speed-up the process, from cell organelle detection to quantification across electron m...
Source: Cell Research - March 19, 2024 Category: Cytology Authors: Neboj ša Nešić Xavier Heiligenstein Lydia Zopf Valentin Bl üml Katharina S Keuenhof Michael Wagner Johanna L H öög Heng Qi Zhiyang Li Georgios Tsaramirsis Christopher J Peddie Milo š Stojmenović Andreas Walter Source Type: research

ATG-101 is a tetravalent PD-L1 ×4-1BB bispecific antibody that stimulates anti-tumor immunity through PD-L1 blockade and PD-L1-directed 4-1BB activation
Cancer Res. 2024 Mar 19. doi: 10.1158/0008-5472.CAN-23-2701. Online ahead of print.ABSTRACTImmune checkpoint inhibitors (ICI) have transformed cancer treatment. However, only a minority of patients achieve a profound response. Many patients are innately resistant while others acquire resistance to ICIs. Furthermore, hepatotoxicity and suboptimal efficacy have hampered the clinical development of agonists of 4-1BB, a promising immune stimulating target. To effectively target 4-1BB and treat diseases resistant to ICIs, we engineered ATG-101, a tetravalent "2+2" PD-L1×4-1BB bispecific antibody. ATG-101 bound PD-L1 and 4-1BB ...
Source: Cell Research - March 19, 2024 Category: Cytology Authors: Hui Yuwen Huajing Wang Tengteng Li Yijing Ren Yun-Kai Zhang Peng Chen Ao Sun Gang Bian Bohua Li David Flowers Marc Presler Kalyanasundaram Subramanian Jia Xue Jingjing Wang Kevin Lynch Jay Mei Xiaowen He Bo Shan Bing Hou Source Type: research

Spindle cell embryonal rhabdomyosarcoma of the prostate in an adult patient: a case report and review of the literature
J Int Med Res. 2024 Mar;52(3):3000605241234755. doi: 10.1177/03000605241234755.ABSTRACTRhabdomyosarcoma of the prostate is a rare mesenchymal tumor that originates from undifferentiated mesenchymal cells. Spindle cell rhabdomyosarcoma is a variant of embryonal rhabdomyosarcoma. The vast majority of these two pathological types occur in children, with only a few adult cases reported to date, and both are associated with poor clinical outcomes. We herein report a case involving a man in his early 40s with spindle cell embryonal rhabdomyosarcoma of the prostate. His chief complaint was difficult urination. The diagnosis was c...
Source: Cell Research - March 19, 2024 Category: Cytology Authors: Hanwen Luo Xiangtao Weng Zibin Zhong Xiongwei Shan Wenli Lin Siyi Li Chiming Gu Source Type: research

A matching-based machine learning approach to estimating optimal dynamic treatment regimes with time-to-event outcomes
Stat Methods Med Res. 2024 Mar 19:9622802241236954. doi: 10.1177/09622802241236954. Online ahead of print.ABSTRACTObservational data (e.g. electronic health records) has become increasingly important in evidence-based research on dynamic treatment regimes, which tailor treatments over time to patients based on their characteristics and evolving clinical history. It is of great interest for clinicians and statisticians to identify an optimal dynamic treatment regime that can produce the best expected clinical outcome for each individual and thus maximize the treatment benefit over the population. Observational data impose v...
Source: Cell Research - March 19, 2024 Category: Cytology Authors: Xuechen Wang Hyejung Lee Benjamin Haaland Kathleen Kerrigan Sonam Puri Wallace Akerley Jincheng Shen Source Type: research