Am J Physiol Renal Physiol; +64 new citations
64 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Physiol Renal Physiol These pubmed results were generated on 2017/07/14PubMed comprises more than millions of citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites. (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - July 14, 2017 Category: Urology & Nephrology Tags: Report Source Type: research

Am J Physiol Renal Physiol; +46 new citations
46 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Physiol Renal Physiol These pubmed results were generated on 2017/07/08PubMed comprises more than millions of citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites. (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - July 9, 2017 Category: Urology & Nephrology Tags: Report Source Type: research

Am J Physiol Renal Physiol; +31 new citations
31 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Physiol Renal Physiol These pubmed results were generated on 2017/07/07PubMed comprises more than millions of citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites. (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - July 7, 2017 Category: Urology & Nephrology Tags: Report Source Type: research

Am J Physiol Renal Physiol; +36 new citations
36 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Physiol Renal Physiol These pubmed results were generated on 2017/07/04PubMed comprises more than millions of citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites. (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - July 4, 2017 Category: Urology & Nephrology Tags: Report Source Type: research

Am J Physiol Renal Physiol; +22 new citations
22 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Physiol Renal Physiol These pubmed results were generated on 2017/06/28PubMed comprises more than millions of citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites. (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - June 29, 2017 Category: Urology & Nephrology Tags: Report Source Type: research

The role of capsaicin-sensitive C-fiber afferent pathways in the control of micturition in spinal intact and spinal cord injured mice.
We examined bladder and urethral sphincter activity in mice with or without spinal cord injury (SCI) after C-fiber afferent desensitization induced by capsaicin pretreatment and changes in electrophysiological properties of mouse bladder afferent neurons 4 weeks after SCI. Female C57BL/6N mice were divided into 4 groups; (1) spinal intact (SI)-control, (2) SI-capsaicin pretreatment (Cap), (3) SCI-control and (4) SCI-Cap groups. Continuous cystometry and external urethral sphincter (EUS)-electromyogram (EMG) were conducted under an awake condition. In Cap groups, capsaicin (25, 50 or 100 mg/kg) was injected subcutaneously 4...
Source: Am J Physiol Renal P... - June 21, 2017 Category: Urology & Nephrology Authors: Kadekawa K, Majima T, Shimizu T, Wada N, de Groat WC, Kanai AJ, Goto M, Yoshiyama M, Sugaya K, Yoshimura N Tags: Am J Physiol Renal Physiol Source Type: research

Biophysics and Biofluiddynamics of Primary Cilia: evidence for and against the flow-sensing function.
Abstract Primary cilia have been called "the forgotten organelle" for over 20 years. As cilia now have their own Journal and several books devoted to their study, perhaps it's time to reconsider the moniker "forgotten organelle". In fact, during the drafting of this review, 12 relevant publications have been issued- we therefore apologize in advance for any relevant work we inadvertently omitted. What purpose is yet another ciliary review? The primary goal of this review is to specifically examine the evidence for and against the hypothesized flow-sensing function of primary cilia expressed by ...
Source: Am J Physiol Renal P... - June 21, 2017 Category: Urology & Nephrology Authors: Nag S, Resnick A Tags: Am J Physiol Renal Physiol Source Type: research

The effects of neuromodulation in a novel obese-prone rat model of detrusor underactivity.
Abstract Obesity is a global epidemic associated with an increased risk for lower urinary tract dysfunction. Inefficient voiding and urinary retention may arise in late-stage obesity when the expulsive force of the detrusor smooth muscle cannot overcome outlet resistance. Detrusor underactivity (DUA) and impaired contractility may contribute to the pathogenesis of non-obstructive urinary retention. We used cystometry and electrical stimulation of peripheral nerves (pudendal and pelvic n.) to characterize and improve bladder function in urethane-anesthetized obese-prone (OP) and obese-resistant (OR) rats following ...
Source: Am J Physiol Renal P... - June 21, 2017 Category: Urology & Nephrology Authors: Gonzalez EJ, Grill WM Tags: Am J Physiol Renal Physiol Source Type: research

Harnessing basic and clinic tools to evaluate SGLT2 inhibitor nephrotoxicity.
Abstract N/A. PMID: 28637789 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - June 21, 2017 Category: Urology & Nephrology Authors: Saly D, Perazella MA Tags: Am J Physiol Renal Physiol Source Type: research

Protection of kidneys by magnesium in cisplatin chemotherapy: a fight between two metals.
Abstract Commentary on: Kumar G, Solanki MH, Xue X, Mintz R, Madankumar S, Chatterjee PK, and Metz CN. Magnesium improves cisplatin-mediated tumor killing while protecting against cisplatin-induced nephrotoxicity. Am J Physiol Renal Physiol ajprenal 00688 02016, 2017. PMID: 28637790 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - June 21, 2017 Category: Urology & Nephrology Authors: Li F, Livingston MJ, Dong Z Tags: Am J Physiol Renal Physiol Source Type: research

Store operated calcium entry suppressed tgf β1-smad3 signaling pathway in glomerular mesangial cells.
STORE OPERATED CALCIUM ENTRY SUPPRESSED TGFβ1-SMAD3 SIGNALING PATHWAY IN GLOMERULAR MESANGIAL CELLS. Am J Physiol Renal Physiol. 2017 Jun 21;:ajprenal.00483.2016 Authors: Chaudhari S, Li W, Wang Y, Jiang H, Ma Y, Davis ME, Zuckerman JE, Ma R Abstract Our previous study demonstrated that the abundance of extracellular matrix proteins was suppressed by store-operated Ca2+ entry in mesangial cells (MCs). The present study was conducted to investigate the underlying mechanism focused on the transforming growth factor beta 1 (TGFβ1) - Smad3 pathway, a critical pathway for ECM expansion in diabeti...
Source: Am J Physiol Renal P... - June 21, 2017 Category: Urology & Nephrology Authors: Chaudhari S, Li W, Wang Y, Jiang H, Ma Y, Davis ME, Zuckerman JE, Ma R Tags: Am J Physiol Renal Physiol Source Type: research

Endothelial markers expressing stromal cells are critical for kidney formation.
Abstract Kidneys are highly vascularized and contain many distinct vascular beds. However, the origins and roles of developing renal endothelial cells in the formation of the kidney are unclear. We have shown that the Foxd1-positive renal stroma gives rise to endothelial marker expressing progenitors that are incorporated within a subset of peritubular capillaries; however, the significance of these cells is unclear. The purpose of this study was to determine whether deletion of Flk1 in the Foxd1 stroma was important for renal development. To that end, we conditionally deleted Flk1 (critical for endothelial cell d...
Source: Am J Physiol Renal P... - May 24, 2017 Category: Urology & Nephrology Authors: Mukherjee E, Maringer KV, Papke E, Bushnell DS, Schaefer CM, Kramann R, Ho J, Humphreys BD, Bates CM, Sims-Lucas S Tags: Am J Physiol Renal Physiol Source Type: research

Spatiotemporal analysis of spontaneous myogenic contractions in the urinary bladder of the rabbit; timing and patterns reflect reported electrophysiology.
Abstract The dynamics of propagating myogenic contractions in the wall of the resting ex vivo urinary bladder of the rabbit were characterised by spatiotemporal maps and related to cyclic variation in intravesical pressure (pves). Patches of propagating contractions (PPCs) enlarged and involuted in near synchrony with peaks in intravesical pressure (pves) (3.85 mean ± 0.3cpm). and were preceded by regions of stretch. The maximum area of the bladder undergoing contraction (55.28±2.65 %) and the sizes of individual PPCs (42.61 ± 1.65 mm2) coincided with the peak in pves. PPCs, originated and pro...
Source: Am J Physiol Renal P... - May 24, 2017 Category: Urology & Nephrology Authors: Hulls CM, Lentle RG, King QM, Reynolds GW, Chambers JP Tags: Am J Physiol Renal Physiol Source Type: research

Special K: Once the fun is over an EMT arrives for the bladder.
Abstract Wang et al. report that in rats exposed to daily ketamine for sixteen weeks, the urothelium begins to undergo phenoconversion to a more mesenchymal state. This epithelial to mesenchymal transition (EMT), has several classic molecular hallmarks and Wang et al. demonstrate convincingly that a number of these including downregulation of E-cadherin, upregulation of mesenchymal markers vimentin and fibroblast specific protein (FSP-1), colocalization of both epithelial and mesenchymal markers in a subset of urothelium-associated cells and upregulation of TGFβ expression in urothelium are all present. ...
Source: Am J Physiol Renal P... - May 24, 2017 Category: Urology & Nephrology Authors: Hill WG, Yu W Tags: Am J Physiol Renal Physiol Source Type: research

Nutritional regulation of renal lipogenic factor expression in mice: Comparison to regulation in the liver and skeletal muscle.
Abstract Regulation of lipogenesis by pathophysiological factors in the liver and skeletal muscle is well understood; however, regulation in the kidney is still unclear. To elucidate nutritional regulation of lipogenic factors in the kidney, we measured the renal expression of lipogenic transcriptional factors and enzymes during fasting and refeeding in chow-fed and high-fat-fed mice. We also examined the regulatory effect of the liver X receptor (LXR) on the expression of lipogenic factors. The renal gene expression of sterol regulatory element-binding protein (SREBP)-1c and fatty acid synthase (FAS) was reduced ...
Source: Am J Physiol Renal P... - May 24, 2017 Category: Urology & Nephrology Authors: Kim SJ, Kim JE, Kim YW, Kim JY, Park SY Tags: Am J Physiol Renal Physiol Source Type: research

Altered expression and modulation of the two-pore domain (K2p) mechano-gated potassium channel TREK-1 in overactive human detrusor.
Conclusions Our data confirmed a significant down-regulation of TREK-1 expression in human DO specimens, and provided evidence of close association between the channel, cell cytoskeleton and caveolins. Up-regulation of TREK-1 expression by saRNA could be a future step for the development of in vivo pharmacological and genetic approaches to treat DO in humans. PMID: 28539337 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - May 24, 2017 Category: Urology & Nephrology Authors: Pineda RH, Nedumaran B, Hypolite JA, Pan XQ, Wilson S, Meacham RB, Malykhina AP Tags: Am J Physiol Renal Physiol Source Type: research

Expression of Trans- and Paracellular Calcium and Magnesium Transport Proteins in Renal and Intestinal Epithelia During Lactation.
In conclusion, proteins involved in transcellular Ca2+ and Mg2+ transport pathways increase during lactation, while expression of paracellular transport proteins remained unchanged. Increased fractional Ca2+ excretion can be explained by vitamin D-dependent intestinal hyperabsorption and bone demineralization, despite enhanced transcellular Ca2+ uptake by the kidney. PMID: 28539338 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - May 24, 2017 Category: Urology & Nephrology Authors: Beggs MR, Appel I, Svenningsen P, Skjødt K, Alexander RT, Dimke H Tags: Am J Physiol Renal Physiol Source Type: research

MIF-2/D-DT Enhances Proximal Tubular Cell Regeneration Through SLPI and ATF4-dependent Mechanisms.
Abstract Macrophage migration inhibitory factor (MIF) is a cytokine with pleiotropic actions that is produced by several organs and cell types. Depending on the target cell and the inflammatory context, MIF can engage its two component receptor complex CD74 and CD44, and the chemokine receptors CXCR2/4. MIF is constitutively expressed in renal proximal tubular cells, stored in intracellular preformed pools and is released at a low rate. Recently a second MIF-like protein (i.e. MIF-2/D-DT) has been characterized in mammals. Our study was aimed at examining the role of MIF-2/D-DT, which mediates tissue protection in...
Source: Am J Physiol Renal P... - May 24, 2017 Category: Urology & Nephrology Authors: Ochi A, Chen D, Schulte W, Leng L, Moeckel NG, Piecychna M, Averdunk L, Stoppe C, Bucala R, Moeckel GW Tags: Am J Physiol Renal Physiol Source Type: research

Cross-sex transplantation alters gene expression and enhances inflammatory response in the transplanted kidneys.
In conclusion, cross-sex-KTX alters gene expression levels and induces inflammatory responses, which might play an important role in long-term graft function. PMID: 28515172 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - May 17, 2017 Category: Urology & Nephrology Authors: Wang L, Song J, Wang S, Buggs J, Chen R, Zhang J, Wang L, Rong S, Li W, Wei J, Liu R Tags: Am J Physiol Renal Physiol Source Type: research

Heme Oxygenase-1 Mitigates Ferroptosis in Renal Proximal Tubule Cells.
Abstract Ferroptosis is an iron-dependent form of regulated, non-apoptotic cell death, which contributes to damage in models of acute kidney injury (AKI). Heme oxygenase-1 (HO-1) is a cytoprotective enzyme induced in response to cellular stress, and is protective against AKI due to its anti-apoptotic and anti-inflammatory properties. However, the role of HO-1 in regulating ferroptosis is unclear. The purpose of this study was to elucidate the role of HO-1 in regulating ferroptotic cell death in renal proximal tubule cells (PTCs). Immortalized PTCs obtained from HO-1+/+ and HO-1-/- mice were treated with erastin or...
Source: Am J Physiol Renal P... - May 17, 2017 Category: Urology & Nephrology Authors: Adedoyin O, Boddu R, Traylor AM, Lever JM, Bolisetty S, George J, Agarwal A Tags: Am J Physiol Renal Physiol Source Type: research

Functional Assessment of Sodium Chloride Co-transporter NCC Mutants in Polarized Mammalian Epithelial Cells.
In conclusion, this system allows the activity, cellular localization, and abundance of wildtype or mutant NCC to be examined in the same polarized mammalian expression system in a rapid, easy, and low cost fashion. PMID: 28515174 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - May 17, 2017 Category: Urology & Nephrology Authors: Rosenbaek LL, Rizzo F, MacAulay N, Staub O, Fenton RA Tags: Am J Physiol Renal Physiol Source Type: research

Role for reactive oxygen species in flow stimulated inner medullary collecting duct endothelin-1 production.
Abstract Inner medullary collecting duct (IMCD)-derived endothelin-1 (ET-1) is stimulated by volume expansion, in part through augmented luminal flow, whereupon it can elicit natriuresis and diuresis. Since flow can alter nitric oxide (NO) and reactive oxygen species (ROS), both of which can affect collecting duct salt transport, we asked whether NO and/or ROS mediate flow-stimulated IMCD ET-1. Mouse IMCD3 cells were exposed to flow and ET-1/GAPDH mRNA assessed. A shear stress of 10 dyne/cm2 for 1 hr increased ET-1 mRNA by 4-fold compared to no flow (ET-1 flow response). Global NO synthase (NOS) inhibition (L-NAME...
Source: Am J Physiol Renal P... - May 17, 2017 Category: Urology & Nephrology Authors: Wheatley W, Kohan DE Tags: Am J Physiol Renal Physiol Source Type: research

Acute Exercise does not Impair Renal Function in Non-Dialysis Chronic Kidney Disease Patients Regardless of Disease Stage.
In conclusion, a single 30-minute moderate-intensity aerobic exercise bout does not impair renal function in non-dialysis CKD patients, regardless of disease stage, supporting the notion that exercise training can be safe in this disease. PMID: 28515176 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - May 17, 2017 Category: Urology & Nephrology Authors: Santana DA, Poortmans JR, Dorea EL, Machado JBA, Fernandes AL, Pinto ALS, Gualano B, Roschel H Tags: Am J Physiol Renal Physiol Source Type: research

Do β3-adrenoceptor agonists cause urinary bladder smooth muscle relaxation by inhibiting acetylcholine release?
Do β3-adrenoceptor agonists cause urinary bladder smooth muscle relaxation by inhibiting acetylcholine release? Am J Physiol Renal Physiol. 2017 May 17;:ajprenal.00215.2017 Authors: Okeke K, Gravas S, Michel MC Abstract The mechanisms leading to improvement of overactive bladder syndrome upon treatment with a β3-adrenoceptor agonist remain controversial. New research points to a role of inhibition of neuronal acetylcholine release. Whether this occurs by a direct effect on prejunctional β3-adrenoceptors or indirectly by formation of adenosine and activation of A1 adenosine receptors is ...
Source: Am J Physiol Renal P... - May 17, 2017 Category: Urology & Nephrology Authors: Okeke K, Gravas S, Michel MC Tags: Am J Physiol Renal Physiol Source Type: research

A Model Specific Role of MicroRNA-223 as a Mediator of Kidney Injury During Experimental Sepsis.
Abstract Sepsis outcomes are heavily dependent on the development of septic organ injury, but no interventions exist to interrupt or reverse this process. MicroRNA-223 (miR-223) is known to be involved in both inflammatory gene regulation and host-pathogen interactions key to the pathogenesis of sepsis. The goal of this study was to determine the role of miR-223 as a mediator of septic kidney injury. Using miR-223 knockout mice and multiple models of experimental sepsis, we found that miR-223 differentially influences acute kidney injury (AKI) based on the model used. In the absence of miR-223, mice demonstrated e...
Source: Am J Physiol Renal P... - May 17, 2017 Category: Urology & Nephrology Authors: Colbert JF, Ford JA, Haeger SM, Yang Y, Dailey KL, Allison KC, Neudecker V, Evans CM, Richardson VL, Brodsky K, Faubel S, Eltzschig HK, Schmidt EP, Ginde AA Tags: Am J Physiol Renal Physiol Source Type: research

Arginase-2 Mediates Renal Ischemia/Reperfusion Injury.
Abstract Novel therapeutic interventions for preventing or attenuating kidney injury following ischemia/reperfusion injury (IRI) remain a focus of significant interest. Currently, there are no definitive therapeutic or preventive approaches available for ischemic acute kidney injury (AKI). Our objective is to determine (a) whether renal arginase activity or expression is increased in renal IRI and (b) whether arginase plays a role in development of renal IRI. The impact of arginase activity and expression on renal damage was evaluated in male C57BL/6J (WT) and arginase-2 deficient (Arg2(-/-) ) mice subjected to bi...
Source: Am J Physiol Renal P... - May 17, 2017 Category: Urology & Nephrology Authors: Raup-Konsavage WM, Gao T, Cooper TK, Morris SM, Reeves WB, Awad AS Tags: Am J Physiol Renal Physiol Source Type: research

ATP-citrate lyase is essential for high glucose-induced histone hyperacetylation and fibrogenic gene up-regulation in mesangial cells.
Abstract TThe goal of this study was to address the role of ATP-citrate lyase (ACL), an enzyme that converts citrate to acetyl-CoA, in high glucose (HG)-induced histone acetylation and pro-fibrotic gene expression. Our recent ChIP-Seq studies have demonstrated that HG induces genome-wide histone hyperacetylation in mesangial cells (MCs). Here we showed that exposure of MCs to HG markedly increased histone acetylation at the H3K9/14 and H3K18 marks and induced the expression of potent pro-fibrotic factors TGF-β1, TGF-β3 and CTGF. The induction of these pro-fibrotic factors was further enhanced by histone ...
Source: Am J Physiol Renal P... - May 10, 2017 Category: Urology & Nephrology Authors: Deb DK, Chen Y, Sun J, Wang Y, Li YC Tags: Am J Physiol Renal Physiol Source Type: research

Reversible plasticity of detrusor smooth muscle: Evidence for a key role of "slipping" actomyosin crossbridges in the control of urinary bladder compliance.
Reversible plasticity of detrusor smooth muscle: Evidence for a key role of "slipping" actomyosin crossbridges in the control of urinary bladder compliance. Am J Physiol Renal Physiol. 2017 May 10;:ajprenal.00227.2017 Authors: Eddinger TJ Abstract none. PMID: 28490527 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - May 10, 2017 Category: Urology & Nephrology Authors: Eddinger TJ Tags: Am J Physiol Renal Physiol Source Type: research

Combining new tools to assess renal function and morphology: a holistic approach to study the effects of aging and a congenital nephron deficit.
etz N Abstract Recently, new methods for assessing renal function in conscious mice (transcutaneous assessment) and for counting and sizing all glomeruli in whole kidneys (MRI) were described. In the present study, these methods were used to assess renal structure and function in aging mice, and in mice born with a congenital low nephron endowment. Age-related nephron loss was analysed in adult C57BL/6 mice (10-50 weeks of age) and congenital nephron deficit was assessed in glial cell line-derived neurotrophic factor heterozygous (GDNF HET) null mutant mice. Renal function was measured through the transcutaneous q...
Source: Am J Physiol Renal P... - May 10, 2017 Category: Urology & Nephrology Authors: Geraci S, Chacón-Caldera J, Cullen-McEwen LA, Schad LR, Sticht C, Puelles VG, Bertram JF, Gretz N Tags: Am J Physiol Renal Physiol Source Type: research

Effect of sodium nitrite on renal function, sodium and water excretion and brachial and central blood pressure in healthy subjects. A dose-response study.
Abstract Sodium nitrite (NaNO2) is converted to nitric oxide (NO) in vivo and has vasodilatory and natriuretic effects. Our aim was to examine the effects of NaNO2 on hemodynamics, sodium excretion and GFR. In a single-blinded, placebo-controlled, cross-over study, we infused placebo (0.9% NaCl) or 0.58, 1.74, or 3.48 μmol NaNO2/kg/hour for two hours in twelve healthy subjects, after four days standard diet. Subjects were supine and water-loaded. We measured brachial and central blood pressure (BP), plasma concentrations of renin, angiotensin II, aldosterone, arginine vasopressin (P-AVP), and plasma nitrite (P-...
Source: Am J Physiol Renal P... - May 10, 2017 Category: Urology & Nephrology Authors: Rosenbaek JB, Therwani SA, Jensen JM, Mose FH, Wandall-Frostholm C, Pedersen EB, Bech JN Tags: Am J Physiol Renal Physiol Source Type: research

IL-6 promotes epithelial-to-mesenchymal transition of human peritoneal mesothelial cells possibly through JAK2/STAT3 signaling pathway.
This study was designed to investigate the role of IL-6 in EMT and peritoneal membrane dysfunction in long-term PD patients by assessing the level of IL-6 in dialysate and exploring the relationship between IL-6, the related signaling pathway JAK2/STAT3, and EMT, using in vitro cellular and molecular techniques. Plasma and dialysate levels of IL-6 were significantly higher in PD ultrafiltration failure patients compared to those in patients without ultrafiltration failure and were negatively correlated with measures of PD adequacy. In vitro, IL-6 treatment changed human peritoneal mesothelial cell phenotype from a typical ...
Source: Am J Physiol Renal P... - May 10, 2017 Category: Urology & Nephrology Authors: Xiao J, Gong Y, Chen Y, Yu D, Wang X, Zhang X, Dou Y, Liu D, Cheng G, Lu S, Yuan W, Li Y, Zhao Z Tags: Am J Physiol Renal Physiol Source Type: research

THE EFFECTS OF ANGIOTENSIN-(1-7) ON THE NHE3 EXCHANGER AND ON [Ca2+]i IN THE PROXIMAL TUBULES OF SPONTANEOUSLY HYPERTENSIVE RATS.
Abstract The acute effects of Ang-(1-7) on the reabsorptive bicarbonate flow (JHCO3-) were evaluated using stationary microperfusion in vivo in the proximal tubules of spontaneously hypertensive rats (SHR) and their normotensive controls, Wistar-Kyoto (WKY) rats, using a microelectrode sensitive to H+. In WKY rats, the control JHCO3- was 2.40 ± 0.10 nmol.cm-2.s-1 (28); Losartan (10-7 M) or A779 (10-6 M, a specific Mas antagonist), alone or in combination with Losartan, decreased the JHCO3-. Ang-(1-7) had biphasic effects on JHCO3-: at 10-9 M, it inhibited and at 10-6, it stimulated the flow. S3226 (10-6 M, ...
Source: Am J Physiol Renal P... - May 10, 2017 Category: Urology & Nephrology Authors: Castelo-Branco R, Leite-Dellova DCA, Fernandes F, Malnic G, Mello-Aires M Tags: Am J Physiol Renal Physiol Source Type: research

Podocyte specific knockout (KO) of cyclooxygenase 2 (COX2) exacerbates diabetic kidney disease.
Abstract Enhanced expression of cyclooxygenase 2 (COX2) in podocytes contributes to glomerular injury in diabetic kidney disease, but some basal level of podocyte COX2 expression might be required to promote podocyte attachment and/or survival. To investigate the role of podocyte COX2 expression in diabetic kidney disease, we deleted COX2 specifically in podocytes in a mouse model of type 1 diabetes mellitus (Akita mice). Podocyte specific knockout (KO) of COX2 did not affect renal morphology or albuminuria in non-diabetic mice. Albuminuria was significantly increased in wild type (WT) and KO Akita mice compared t...
Source: Am J Physiol Renal P... - May 10, 2017 Category: Urology & Nephrology Authors: Wang L, Sha Y, Bai J, Eisner W, Sparks MA, Buckley AF, Spurney RF Tags: Am J Physiol Renal Physiol Source Type: research

The Tryptophan-Kynurenine Pathway, Systemic Inflammation, and Long-Term Outcome after Kidney Transplantation.
Abstract BACKGROUND: Tryptophan is metabolized along the kynurenine pathway, initially to kynurenine, and subsequently to cytotoxic 3-hydroxykynurenine. There is increasing interest in this pathway, because of its pro-inflammatory nature, and drugs interfering in it receive increasing attention. We aimed to investigate whether serum and urinary parameters of the tryptophan-kynurenine pathway, and particularly cytotoxic 3-hydroxykynurenine, are associated with systemic inflammation and long-term outcome in renal transplant recipients (RTR). METHODS: Data were collected in outpatient RTR with a functioning graf...
Source: Am J Physiol Renal P... - May 10, 2017 Category: Urology & Nephrology Authors: de Vries LV, Minovic I, Franssen CF, van Faassen M, Sanders JF, Berger SP, Navis G, Kema IP, Bakker SJ Tags: Am J Physiol Renal Physiol Source Type: research

Urine RAS components in mice and people with type 1 diabetes and chronic kidney disease.
Abstract Pathways implicated in diabetic kidney disease (DKD) are largely derived from animal models. To examine if renin-angiotensin system (RAS) changes in humans are concordant with rodent models, we measured concentration of angiotensinogen (AOG), cathepsin D(CTSD), angiotensin converting enzyme(ACE), ACE2 and enzymatic activities of ACE, ACE2 and aminopeptidase-A in FVB mice 13-20 weeks after streptozotocin (n=9) or vehicle (n=15) and people with longstanding type 1 diabetes, with (n=37) or without (n=81) DKD. In streptozotocin-treated mice, urine AOG and CTSD were 10.4-and 3.0-fold higher than controls, respectively (p-values
Source: Am J Physiol Renal P... - May 3, 2017 Category: Urology & Nephrology Authors: Wysocki J, Goodling A, Burgaya M, Whitlock K, Ruzinski J, Batlle D, Afkarian M Tags: Am J Physiol Renal Physiol Source Type: research

Ovariectomy Uncovers Purinergic Receptor Activation of Endothelin-dependent Natriuresis.
Abstract We recently reported that natriuresis produced by renal medullary salt loading is dependent on endothelin (ET)-1 and purinergic (P2) receptors in male rats. Since sex differences in ET-1 and P2 signaling have been reported, we decided to test whether ovarian sex hormones regulate renal medullary ET-1 and P2-dependent natriuresis. The effect of medullary NaCl loading on Na+ excretion was determined in intact and ovariectomized (OVX) female Sprague Dawley rats with and without ET-1 or P2 receptor antagonism. Isosmotic saline (284 mOsmol/kg H2O) was infused into the renal medullary interstitium of anesthetiz...
Source: Am J Physiol Renal P... - May 3, 2017 Category: Urology & Nephrology Authors: Gohar EY, Kasztan M, Becker BK, Speed JS, Pollock DM Tags: Am J Physiol Renal Physiol Source Type: research

Coping with nephron loss: transport at a price.
Abstract EF - F-00018-2017. PMID: 28468963 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - May 3, 2017 Category: Urology & Nephrology Authors: Weinstein AM Tags: Am J Physiol Renal Physiol Source Type: research

Luminal angiotensin II is internalized as a complex with AT1R/AT2R heterodimers to target endoplasmic reticulum in LLC-PK1 cells.
owe J Abstract Angiotensin II (ANG II) has many biological effects in renal physiology, particularly in Ca(2+) handling in the regulation of fluid and solute reabsorption. It involves the systemic endocrine renin-angiotensin system (RAS), but tissue and intracrine ANG II are also known. We have shown that ANG II induces heterodimerization of its AT1 and AT2 receptors (AT1R and AT2R) to stimulate sarco(endo)plasmic reticulum Ca(2+)-ATPase (SERCA) activity. Thus, we investigated whether ANG II-AT1R/AT2R complex is formed and internalized, and also looked at the intracellular localization of this complex to determine...
Source: Am J Physiol Renal P... - May 3, 2017 Category: Urology & Nephrology Authors: Ferrão FM, Cardoso LHD, Drummond HA, Li XC, Zhuo JL, Gomes DS, Lara LS, Vieyra A, Lowe J Tags: Am J Physiol Renal Physiol Source Type: research

Cell specific qRT-PCR of renal epithelial cells reveals a novel innate immune signature in murine collecting duct.
Abstract The urinary tract is usually culture negative despite its close proximity to microbial flora. The precise mechanisms by which the kidneys and urinary tract defends against infection is not well understood. The initial kidney cells to encounter ascending pathogens are the collecting tubule cells which consist of principal cells (PCs) that express aquaporin 2 (AQP2) and intercalated cells (ICs) that express vacuolar H+-ATPase (V-ATPase, B1 subunit). We have previously shown that ICs are involved with the human renal innate immune defense. Here we generated two reporter mice, VATPase B1-cre+tdT+ mice to fluo...
Source: Am J Physiol Renal P... - May 3, 2017 Category: Urology & Nephrology Authors: Saxena V, Hains DS, Ketz J, Chanley M, Spencer JD, Becknell B, Pierce KR, Nelson RD, Purkerson JM, Schwartz GJ, Schwaderer AL Tags: Am J Physiol Renal Physiol Source Type: research

Protein Disulfide Isomerase Regulates Renal AT1 Receptor Function and Blood Pressure in Rats.
Abstract Role and mechanism of renal PDI in blood pressure (BP) regulation has not been tested before. Here, we are testing this possibility in Sprague-Dawley (SD) rats. Rats were treated with PDI inhibitor bacitracin (100mg/kg, i.p./day for 14 days) followed by determining BP and renal AT1 receptor (AT1R) function in anesthetized rats. Renal AT1R function was determined as the ability of candesartan (an AT1R blocker) to increase diuresis and natriuresis. Second set of vehicle and bacitracin treated rats were used to determine biochemical parameters. Systolic BP increased in bacitracin-treated compared to vehicle-...
Source: Am J Physiol Renal P... - May 3, 2017 Category: Urology & Nephrology Authors: Wang X, Asghar M Tags: Am J Physiol Renal Physiol Source Type: research

Corrigendum.
Authors: PMID: 28428335 [PubMed - in process] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - April 22, 2017 Category: Urology & Nephrology Tags: Am J Physiol Renal Physiol Source Type: research

Corrigendum.
Authors: PMID: 28428336 [PubMed - in process] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - April 22, 2017 Category: Urology & Nephrology Tags: Am J Physiol Renal Physiol Source Type: research

Adaptive Changes in GFR, Tubular Morphology and Transport in Subtotal Nephrectomized Kidneys: Modeling and Analysis.
Abstract Removal of renal mass stimulates anatomical and functional adaptations in the surviving nephrons, including elevation in single-nephron glomerular filtration rate (SNGFR) and tubular hypertrophy. A goal of this study is to assess the extent to which the concomitant increases in filtered load and tubular transport capacity preserve homeostasis of water and salt. To accomplish that goal, we developed computational models to simulate solute transport and metabolism along nephron populations in a uninephrectomized (UNX) rat and a 5/6-nephrectomized (5/6-NX) rat. Model simulations indicate that nephrectomy-ind...
Source: Am J Physiol Renal P... - March 22, 2017 Category: Urology & Nephrology Authors: Layton AT, Edwards A, Vallon V Tags: Am J Physiol Renal Physiol Source Type: research

Proximal Tubule Glutamine Synthetase Expression is Necessary for the Normal Response to Dietary Protein Restriction.
Abstract Dietary protein restriction has multiple benefits in kidney disease. Because protein intake is a major determinant of endogenous acid production, it is important that net acid excretion change in parallel during changes in dietary protein intake. Dietary protein restriction decreases endogenous acid production and ¬decreases urinary ammonia excretion, a major component of net acid excretion. Glutamine synthetase (GS) catalyzes the reaction of NH4+ and glutamate, which regenerates the essential amino acid glutamine and decreases net ammonia generation. Because renal proximal tubule GS expression increa...
Source: Am J Physiol Renal P... - March 22, 2017 Category: Urology & Nephrology Authors: Lee HW, Osis G, Handlogten ME, Verlander JW, Weiner ID Tags: Am J Physiol Renal Physiol Source Type: research

An Intracellular Matrix Metalloproteinase-2 Isoform Induces Tubular Regulated Necrosis: Implications for Acute Kidney Injury.
Abstract Acute kidney injury (AKI) causes severe morbidity, mortality, and chronic kidney disease (CKD). Mortality is particularly marked in the elderly and with pre-existing CKD. Oxidative stress is a common theme in models of AKI induced by ischemia/reperfusion (I/R) injury. We recently characterized an intracellular isoform of matrix metalloproteinase-2 (MMP-2) induced by oxidative stress-mediated activation of an alternate promoter in the first intron of the MMP-2 gene. This generates an N-terminal truncated MMP-2 isoform (NTT-MMP-2) that is intracellular and associated with mitochondria. The NTT-MMP-2 isoform...
Source: Am J Physiol Renal P... - March 22, 2017 Category: Urology & Nephrology Authors: Ceron CS, Baligand C, Joshi SK, Wanga S, Cowley PM, Walker JP, Song SH, Mahimkar R, Baker AJ, Raffai RL, Wang ZJ, Lovett DH Tags: Am J Physiol Renal Physiol Source Type: research

Hypoxia inducible factor-1 α activation improves renal oxygenation and mitochondrial function in early chronic kidney disease.
In conclusion, we report significant impairment of renal oxygenation and mitochondrial function at the early stages of CKD and demonstrate the beneficial role of HIF-1α activation on renal function and metabolism. PMID: 28331062 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - March 22, 2017 Category: Urology & Nephrology Authors: Thomas JL, Pham H, Li Y, Hall E, Perkins GA, Ali SS, Patel HH, Singh P Tags: Am J Physiol Renal Physiol Source Type: research

MicroRNA-148b regulates megalin expression and is associated with receptor downregulation in mice with unilateral ureteral obstruction.
Abstract Megalin is a multiligand, endocytic receptor important for the normal, proximal tubule reabsorption of filtered proteins, hormones, enzymes, essential nutrients, and nephrotoxins. Megalin dysfunction has been associated with acute as well as chronic kidney diseases. Tubular proteinuria has been observed following unilateral ureteral obstruction (UUO) suggesting megalin dysfunction; however, the pathophysiological mechanism has not been resolved. In order to identify potential regulators of megalin expression, we examined renal microRNAs (miRNAs) expression and observed an upregulation of microRNA-148b (mi...
Source: Am J Physiol Renal P... - March 22, 2017 Category: Urology & Nephrology Authors: Wen L, Andersen PK, Husum DM, Norregaard R, Zhao Z, Liu Z, Birn H Tags: Am J Physiol Renal Physiol Source Type: research

Maternal Separation Diminishes Alpha-Adrenergic Receptors Density and Function in Renal Vasculature from male Wistar Kyoto rats.
Abstract Adult rats exposed to maternal separation (MatSep) are normotensive but display lower glomerular filtration rate and increased renal neuroadrenergic drive. The aim of this study was to determine the renal alpha-adrenergic receptor density and the renal vascular responsiveness to adrenergic stimulation in male rats exposed to MatSep. In addition, baroreflex sensitivity was assessed to determine a component of neural control of the vasculature. Using tissue collected from 4-month-old MatSep and control rats, alpha 1-adrenergic receptors (α1-ARs) were measured in renal cortex and isolated renal vascula...
Source: Am J Physiol Renal P... - March 22, 2017 Category: Urology & Nephrology Authors: Loria AS, Osborn JL Tags: Am J Physiol Renal Physiol Source Type: research

Urothelial proliferation and regeneration after spinal cord injury.
Abstract The basal, intermediate, and superficial cell layers of the urothelium undergo rapid and complete recovery following acute injury; however, the effects of chronic injury on urothelial regeneration have not been well defined. To address this discrepancy, we employed a mouse model to explore urothelial changes in response to spinal cord injury (SCI), a condition characterized by life-long bladder dysfunction. One-day post SCI there was a focal loss of umbrella cells, which are large cells that populate the superficial cell layer and normally express uroplakins (UPKs) and KRT20, but not KRT5, KRT14, or TP63....
Source: Am J Physiol Renal P... - March 22, 2017 Category: Urology & Nephrology Authors: Kullmann FA, Clayton DR, Ruiz WG, Wolf-Johnson A, Gauthier C, Kanai AJ, Birder LA, Apodaca G Tags: Am J Physiol Renal Physiol Source Type: research

Ketamine-induced bladder fibrosis involves epithelial-to-mesenchymal transition mediated by transforming growth factor- β1.
Ketamine-induced bladder fibrosis involves epithelial-to-mesenchymal transition mediated by transforming growth factor-β1. Am J Physiol Renal Physiol. 2017 Mar 22;:ajprenal.00686.2016 Authors: Wang J, Chen Y, Gu D, Zhang G, Chen J, Zhao J, Wu P Abstract Bladder wall fibrosis is a major complication of ketamine-induced cystitis (KC), but the underlying pathogenesis is poorly understood. The aim of the present study was to elucidate the mechanism of ketamine-induced fibrosis in association with epithelial-to-mesenchymal transition (EMT) mediated by transforming growth factor-β1 (TGF-β1). ...
Source: Am J Physiol Renal P... - March 22, 2017 Category: Urology & Nephrology Authors: Wang J, Chen Y, Gu D, Zhang G, Chen J, Zhao J, Wu P Tags: Am J Physiol Renal Physiol Source Type: research