ENaC inhibition stimulates HCl secretion in the mouse cortical collecting duct II: Bafilomycin-sensitive H+ secretion.
CONCLUSIONS: 1) in CCDs from aldosterone-treated mice, benzamil application stimulates HCl secretion mediated by the apical H(+)-ATPase and a yet to be identified conductive Cl(-) transport pathway. 2) Benzamil-induced HCl secretion is reversed with the application of stilbene inhibitors or H+-ATPase inhibitors to the perfusate. 3) Benzamil reduces VT not only by inhibiting conductive Na+ absorption, but also by stimulating H(+) secretion. PMID: 26017972 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - May 27, 2015 Category: Urology & Nephrology Authors: Nanami M, Pech V, Lazo-Fernandez Y, Weinstein AM, Wall SM Tags: Am J Physiol Renal Physiol Source Type: research

Role of Brainstem in Tibial Inhibition of the Micturition Reflex in Cats.
This study examined the role of the brainstem in inhibition of bladder reflexes induced by tibial nerve stimulation (TNS) in α-chloralose anesthetized decerebrate cats. Repeated cystometrograms (CMGs) were performed by infusing saline or 0.25% acetic acid (AA) to elicit normal or overactive bladder reflexes, respectively. TNS (5 or 30 Hz) at three times threshold (3T) intensity for inducing toe movement was applied for 30 min between CMGs to induce post-TNS inhibition or applied during the CMGs to induce acute TNS inhibition. Inhibition was evident as an increase in bladder capacity without a change in amplitude of b...
Source: Am J Physiol Renal P... - May 27, 2015 Category: Urology & Nephrology Authors: Ferroni MC, Slater RC, Shen B, Xiao Z, Wang J, Lee A, Roppolo JR, de Groat WC, Tai C Tags: Am J Physiol Renal Physiol Source Type: research

Changes In Glomerular Parietal Epithelial Cells In Mouse Kidneys With Advanced Age.
Abstract Kidney aging is accompanied by characteristic changes in the glomerulus, but little is known about the effect of aging on glomerular parietal epithelial cells (PECs), nor if the characteristic glomerular changes in man and rats also occur in very old mice. Accordingly, a descriptive analysis was undertaken in 27-month-old C57B6 mice, considered advanced age. PEC density was significantly lower in older mice compared to young mice (aged 3 months), and the decrease was more pronounced in juxta-medullary glomeruli compared with outer-cortical glomeruli. In addition to segmental and global glomerulosclerosis ...
Source: Am J Physiol Renal P... - May 27, 2015 Category: Urology & Nephrology Authors: Roeder SS, Stefanska A, Eng DG, Kaverina NV, Sunseri MW, McNicholas BA, Rabinovitch P, Engel FB, Daniel C, Amann K, Lichtnekert J, Pippin JW, Shankland SJ Tags: Am J Physiol Renal Physiol Source Type: research

Pleiotropic signaling evoked by tumor necrosis factor in podocytes.
Abstract Tumor necrosis factor (TNF) has been implicated in glomerular diseases but its actions on podocytes are not well understood. Endogenous TNF expression is markedly increased in mouse podocytes exposed to sera from patients with recurrent focal and segmental glomerulosclerosis, and TNF is able to increase its own expression in these cells. Exposing podocytes to TNF increased phosphorylation of NFκB p65-RelA, followed by increased tyrosine phosphorylation of STAT3. STAT3 activation was blocked by the NFκB inhibitor JSH-23 and by the STAT3 inhibitor stattic, whereas TNF-evoked NFκB activatio...
Source: Am J Physiol Renal P... - May 27, 2015 Category: Urology & Nephrology Authors: Abkhezr M, Kim EY, Roshanravan H, Nikilos F, Thomas C, Hagmann H, Benzing T, Dryer SE Tags: Am J Physiol Renal Physiol Source Type: research

Molecular Nephropathology: Ready for Prime Time?
Abstract In the current era of precision medicine, the existing nephropathology paradigm of light microscopy, immunofluorescence, and electron microscopy will become increasingly insufficient. There will be an expectation to supplement these traditional diagnostic tools with patient-specific information related to a growing understanding of molecular pathophysiology. Next generation sequencing technologies are expected to play a key role in the future of nephropathology, but transcriptomics is poised to represent the first major foray into routine molecular testing. The introduction of molecular techniques into cl...
Source: Am J Physiol Renal P... - May 27, 2015 Category: Urology & Nephrology Authors: Adam B, Mengel M Tags: Am J Physiol Renal Physiol Source Type: research

Inhibition of COX-1 attenuates the formation of thromboxane A2 and ameliorates the acute decrease in glomerular filtration rate in endotoxemic mice.
This study demonstrates that endotoxemia-induced thromboxane A2 formation depends on the activity of COX-1. Our study further indicates that the COX-1 inhibitor SC-560 has a protective effect on the decrease in renal function in response to endotoxin. Therefore, our data support a role for thromboxane A2 in the development of AKI in response to LPS. PMID: 26017977 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - May 27, 2015 Category: Urology & Nephrology Authors: Mederle K, Meurer M, Castrop H, Höcherl K Tags: Am J Physiol Renal Physiol Source Type: research

Chronic kidney disease induced by adenine. A suitable model of growth retardation in uremia.
In conclusion, adenine-induced CKD in young rats is associated with growth retardation and disturbed endochondral ossification. This animal protocol may be a useful new experimental model to study growth in CKD. PMID: 25972508 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - May 13, 2015 Category: Urology & Nephrology Authors: Claramunt D, Gil-Peña H, Fuente R, García-López E, Loredo V, Hernández Frías O, Ordóñez FA, Rodríguez-Suárez J, Santos F Tags: Am J Physiol Renal Physiol Source Type: research

Incipient renal transplant dysfunction associates with tubular syndecan-1 expression and shedding.
Abstract Syndecan-1 is a transmembrane heparan sulfate proteoglycan involved in regenerative growth and cellular adhesion. We hypothesize that induction of tubular syndecan-1 is a repair response to incipient renal damage in apparently stable, uncomplicated renal transplant recipients. We quantified tubular syndecan-1 in unselected renal protocol biopsies taken one year after transplantation. Spearman rank correlation analysis revealed an inverse correlation between tubular syndecan-1 expression and creatinine clearance at time of biopsy (r = -0.483; p
Source: Am J Physiol Renal P... - May 13, 2015 Category: Urology & Nephrology Authors: Adepu S, Rosman CW, Dam W, van Dijk MC, Navis G, van Goor H, Bakker SJ, van den Born J Tags: Am J Physiol Renal Physiol Source Type: research

Aberrant glomerular filtration of urokinase-plasminogen activator in nephrotic syndrome leads to amiloride-sensitive plasminogen activation in urine.
In conclusion, nephrotic syndrome is associated with aberrant filtration of uPA across the injured glomerular barrier. Amiloride inhibits urine uPA activity which attenuates plasminogen activation and urine protease activity in vivo. Urine uPA is a relevant target for amiloride in vivo. PMID: 25972510 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - May 13, 2015 Category: Urology & Nephrology Authors: Stæhr M, Buhl KB, Andersen RF, Svenningsen P, Nielsen F, Hinrichs GR, Bistrup C, Jensen BL Tags: Am J Physiol Renal Physiol Source Type: research

Continence and Micturition - Physiologic Mechanisms Under Behavioral Control.
PMID: 25972511 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - May 13, 2015 Category: Urology & Nephrology Authors: Chai TC Tags: Am J Physiol Renal Physiol Source Type: research

Adaptation by the collecting duct to an exogenous acid load is blunted by deletion of the proton-sensing receptor GPR4.
Abstract We previously reported that the deletion of the pH sensor GPR4 causes a non-gap metabolic acidosis and defective net acid excretion (NAE) in the GPR4 knockout mouse (GPR4-/-). Since the major regulatory site of NAE in the kidney is the collecting duct (CD), we examined acid-base transport proteins in intercalated cells of CD and found comparable mRNA expression of kAE1 (anion exchanger 1), pendrin, and a4 subunit of H+-ATPase in GPR4-/-vs.+/+. However, NH4Cl loading elicited adaptive doubling of AE1 mRNA in GPR4+/+, but a 50% less pronounced response in GPR4-/-. In GPR4+/+, NH4Cl loading evoked a cellular...
Source: Am J Physiol Renal P... - May 13, 2015 Category: Urology & Nephrology Authors: Sun X, Stephens L, DuBose TD, Petrovic S Tags: Am J Physiol Renal Physiol Source Type: research

Pendrin gene ablation alters ENaC subcellular distribution and open probability.
This study explored whether the intercalated cell Cl(-)/HCO3 (-) exchanger, pendrin, modulates ENaC function by changing channel open probability and/or channel density. To do so, we measured ENaC subunit subcellular distribution by immunohistochemistry, single channel recordings in split open cortical collecting ducts (CCDs), as well as transepithelial voltage (VT), and Na(+) absorption (JNa) in CCDs from aldosterone-treated wild type and pendrin null mice. Because pendrin gene ablation reduced 70 more than 85 kD γ ENaC band density, we asked if pendrin gene ablation interferes with ENaC cleavage. We observed that E...
Source: Am J Physiol Renal P... - May 13, 2015 Category: Urology & Nephrology Authors: Pech V, Wall SM, Nanami M, Bao HF, Kim YH, Lazo-Fernandez Y, Yue Q, Pham TD, Eaton DC, Verlander JW Tags: Am J Physiol Renal Physiol Source Type: research

Am J Physiol Renal Physiol; +24 new citations
24 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Physiol Renal Physiol These pubmed results were generated on 2015/05/03PubMed comprises more than 24 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites. (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - May 3, 2015 Category: Urology & Nephrology Tags: Report Source Type: research

Evaluation of Voiding Assays in Mice: Impact of Genetic Strains, Gender.
Abstract Void spot assays (VSA) and cystometry are two of the most common tests performed in mice, to assess lower urinary tract function. Assay protocols and methodology vary greatly among laboratories, and little is known about reproducibility of results generated by different laboratories. We performed VSA in four mouse strains, comparing males with females and comparing results between two independent laboratories. Unique aspects of the current study include direct comparison of results of VSA performed in a similar manner in two locations and comparison of cystometry performed using two different rates of inf...
Source: Am J Physiol Renal P... - April 22, 2015 Category: Urology & Nephrology Authors: Bjorling DE, Wang Z, Vezina CM, Ricke WA, Keil KP, Yu W, Guo L, Zeidel M, Hill WG Tags: Am J Physiol Renal Physiol Source Type: research

Urinary Vimentin mRNA as a Potential Novel Biomarker of Renal Fibrosis.
Abstract Renal fibrosis is a histological outcome of chronic kidney disease (CKD) progression. However, the non-invasive detection of renal fibrosis remains a challenge. Here we constructed a renal fibrosis target mRNA array and used it to detect urinary mRNAs of CKD patients for investigating potential non-invasive biomarkers of renal fibrosis. We collected urine samples from 39 biopsy-proven CKD patients and 11 healthy controls in the training set. Urinary mRNA profiles of 86 genes showed a total of 22 mRNAs that were differentially expressed between CKD patients and controls (P
Source: Am J Physiol Renal P... - April 22, 2015 Category: Urology & Nephrology Authors: Cao YH, Lv LL, Zhang X, Hu H, Ding LH, Yin D, Zhang YZ, Ni HF, Chen PS, Liu BC Tags: Am J Physiol Renal Physiol Source Type: research

Melatonin Pre-Treatment of Human Adipose Tissue-Derived Mesenchymal Stromal Cells Enhances their Pro-survival and Protective Effects on Human Kidney Cells.
Abstract The efficacy of cell therapy for many diseases can be limited by the poor survival of implanted cells in an environment of tissue injury. Melatonin has been reported to have anti-oxidative and anti-apoptotic effects. Adipose tissue-derived mesenchymal stromal cells (ASCs), cells easily obtained in high amounts and with minimal discomfort, have shown great promise in cell therapy applications, such as in acute kidney injury. We hypothesized that melatonin pre-treatment of human ASCs (hASCs) would improve their renoprotective and pro-survival effects. We therefore investigated the action of melatonin on hAS...
Source: Am J Physiol Renal P... - April 22, 2015 Category: Urology & Nephrology Authors: Zhao J, Young YK, Fradette J, Eliopoulos N Tags: Am J Physiol Renal Physiol Source Type: research

Hyperglycemia in the absence of cilia accelerates cystogenesis and induces renal damage.
HYPERGLYCEMIA IN THE ABSENCE OF CILIA ACCELERATES CYSTOGENESIS AND INDUCES RENAL DAMAGE. Am J Physiol Renal Physiol. 2015 Apr 22;:ajprenal.00652.2014 Authors: Sas KM, Yin H, Fitzgibbon WR, Baicu CF, Zile MR, Steele SL, Amria MY, Saigusa T, Funk JA, Bunni MA, Siegal GP, Siroky BJ, Bissler JJ, Bell PD Abstract In polycystic kidney disease (PKD) the rate of cyst formation and disease progression is highly variable. The lack of predictability in disease progression may be due to additional environmental factors or pathophysiological processes called "third-hits". Diabetes is a growing epidemic a...
Source: Am J Physiol Renal P... - April 22, 2015 Category: Urology & Nephrology Authors: Sas KM, Yin H, Fitzgibbon WR, Baicu CF, Zile MR, Steele SL, Amria MY, Saigusa T, Funk JA, Bunni MA, Siegal GP, Siroky BJ, Bissler JJ, Bell PD Tags: Am J Physiol Renal Physiol Source Type: research

Tubular Cross-Talk in Acute Kidney Injury: a Story of Sense and Sensibility.
Abstract The mammalian kidney is an organ composed of numerous functional units or nephrons. Beyond the filtering glomerulus of each nephron, various tubular segments with distinct populations of epithelial cells sequentially span the kidney from cortex to medulla. The highly organized folding of the tubules results in a spatial distribution that allows intimate contact between various tubular sub-segments. This unique arrangement can promote a newly recognized type of horizontal epithelial-to- epithelial cross-talk. In this review, we will discuss the importance of this tubular cross-talk in shaping the response ...
Source: Am J Physiol Renal P... - April 15, 2015 Category: Urology & Nephrology Authors: El-Achkar TM, Dagher PC Tags: Am J Physiol Renal Physiol Source Type: research

Candidate genes for hypertension: Insights from the Dahl S rat.
Abstract Human genetic linkage and association studies have nominated many genes as possible contributors to disease. Mutating or deleting these genes in a relevant disease model can validate their association with disease and potentially uncover novel mechanisms of pathogenesis. Targeted genetic mutagenesis has only recently been developed in the rat, and this technique has been applied in the Dahl salt-sensitive (S) rat to investigate human candidate genes associated with hypertension. This mini-review communicates the findings of these studies and displays how targeted genetic mutagenesis may contribute to the ...
Source: Am J Physiol Renal P... - April 15, 2015 Category: Urology & Nephrology Authors: Rudemiller NP, Mattson DL Tags: Am J Physiol Renal Physiol Source Type: research

Impaired Natriuretic Response to High-NaCl Diet plus Aldosterone Infusion in Mice Over-expressing Human CD39, an Ectonucleotidase (NTPDase1).
Abstract Extracellular nucleotides acting through P2 receptors facilitate natriuresis. To define how purinergic mechanisms are involved in sodium homeostasis, we used transgenic mice (TG) globally over-expressing human CD39 (hCD39, NTPDase1), an ectonucleotidase which hydrolyzes extracellular ATP/ADP to AMP, resulting in altered extracellular purine profile. On a high-sodium diet (HSD, 3.5% Na(+)), urine volume and serum sodium were elevated in TG mice without altering sodium excretion; however, there was significant impairment in HSD-induced decrease in urinary aldosterone in TG mice. Western analysis revealed si...
Source: Am J Physiol Renal P... - April 15, 2015 Category: Urology & Nephrology Authors: Zhang Y, Robson SC, Morris KL, Heiney KM, Dwyer KM, Kishore BK, Ecelbarger CM Tags: Am J Physiol Renal Physiol Source Type: research

Angiotensin AT2 receptor agonist prevents salt-sensitive hypertension in obese Zucker rats.
Abstract High sodium intake is a risk factor for the pathogenesis of hypertension, especially in obesity. The present study is designed to investigate whether angiotensin type-2 receptor activation with selective agonist C21 prevents high-sodium diet (HSD)-induced hypertension in obese animals. Male obese rats were treated with AT2R agonist C21 (1 mg/kg/day, oral) while maintained on either normal-sodium diet (NSD, 0.4%) or HSD (4%) for 2-weeks. Radio-telemetric recording showed a time dependent increase in systolic blood pressure in HSD-fed obese rats, being maximal increase (~27 mmHg) at day 12 of the HSD regime...
Source: Am J Physiol Renal P... - April 8, 2015 Category: Urology & Nephrology Authors: Ali Q, Patel SN, Hussain T Tags: Am J Physiol Renal Physiol Source Type: research

Modeling Oxygen Consumption in the Proximal Tubule: Effects of NHE and SGLT2 inhibition.
The objective of this study was to investigate how physiological, pharmacological, and pathological conditions that alter sodium reabsorption (TNa) in the proximal tubule affect oxygen consumption (QO2) and Na(+) transport efficiency (TNa/QO2). To do so, we expanded a mathematical model of solute transport in the proximal tubule of the rat kidney. The model represents compliant S1, S2 and S3 segments, and accounts for their specific apical and basolateral transporters. Sodium is reabsorbed transcellularly, via apical Na(+)/H(+) exchangers (NHE) and Na(+)-glucose (SGLT) cotransporters, and paracellularly. Our results sugges...
Source: Am J Physiol Renal P... - April 8, 2015 Category: Urology & Nephrology Authors: Layton AT, Vallon V, Edwards A Tags: Am J Physiol Renal Physiol Source Type: research

Impact of a folic acid enriched diet on urinary tract function in mice treated with testosterone and estradiol.
Abstract Aging men are susceptible to developing lower urinary tract symptoms, but the underlying etiology is unknown and the influence of dietary and environmental factors on them is unclear. We tested whether a folic acid enriched diet changed urinary tract physiology and biology in control male mice and male mice with urinary dysfunction induced by exogenous testosterone and estradiol (T+E2), which mimics changing hormone levels in aging humans. T+E2 treatment increased mouse urine output, time between voiding events, bladder capacity and compliance. Consumption of a folic acid enriched diet moderated these cha...
Source: Am J Physiol Renal P... - April 8, 2015 Category: Urology & Nephrology Authors: Keil KP, Abler LL, Altmann HM, Wang Z, Wang P, Ricke WA, Bjorling DE, Vezina CM Tags: Am J Physiol Renal Physiol Source Type: research

Antenatal glucocorticoid treatment alters sodium uptake in renal proximal tubule cells from adult offspring in a sex specific manner.
ANTENATAL GLUCOCORTICOID TREATMENT ALTERS SODIUM UPTAKE IN RENAL PROXIMAL TUBULE CELLS FROM ADULT OFFSPRING IN A SEX SPECIFIC MANNER. Am J Physiol Renal Physiol. 2015 Apr 1;:ajprenal.00047.2015 Authors: Su Y, Bi J, Pulgar VM, Figueroa JP, Chappell MC, Rose JC Abstract We have shown a sex specific effect of fetal programming on sodium excretion in adult sheep. The site of this effect in the kidney is unknown. Therefore, we tested the hypothesis that renal proximal tubule cells (RPTC) from adult male sheep exposed to betamethasone (Beta) before birth have greater sodium uptake than do RPTC from vehicle ...
Source: Am J Physiol Renal P... - April 1, 2015 Category: Urology & Nephrology Authors: Su Y, Bi J, Pulgar VM, Figueroa JP, Chappell MC, Rose JC Tags: Am J Physiol Renal Physiol Source Type: research

Kidney adysplasia and variable hydronephrosis, a new mutation affecting the odd-skipped related 1 gene in the mouse.
We describe here a new recessive mutation (variable hydronephrosis, vhn) in the mouse that leads to down regulation of Osr1 transcript, causing several kidney defects: agenesis, hypoplasia and hydronephrosis with variable age of onset. The mutation is closely associated with a reciprocal translocation, T(12;17)4Rk, whose Chr 12 breakpoint is upstream from Osr1. This breakpoint may cause the genetic lesion. The vhn/vhn mutant provides a model to study kidney development and test therapies for hydronephrosis. PMID: 25834070 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - April 1, 2015 Category: Urology & Nephrology Authors: Davisson MT, Cook SA, Akeson EC, Liu D, Heffner C, Gudis P, Fairfield H, Murray SA Tags: Am J Physiol Renal Physiol Source Type: research

Platelets are relevant mediators of renal injury induced by primary endothelial lesions.
In conclusion, platelets are relevant mediators of renal injury induced by primary endothelial lesions early on as demonstrated by platelet depletion as well as platelet inhibition via the P2Y12 receptor. While strategies to prevent platelet-endothelial interaction showed protective effects, platelets' contribution during renal regeneration remains unknown. PMID: 25834071 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - April 1, 2015 Category: Urology & Nephrology Authors: Schwarzenberger C, Sradnick J, Lerea KM, Goligorsky MS, Nieswandt B, Hugo CP, Hohenstein B Tags: Am J Physiol Renal Physiol Source Type: research

Activation of Nuclear Factor of Activated T Cells 5 in the peritoneal membrane of uremic patients.
CONCLUSION: "Uremia" results in NFAT5 induction, which might promote early changes of the peritoneum. Up-regulation of NFAT5 in PD-patients is associated with NFκB induction, potentially resulting in the recruitment of macrophages. PMID: 25834072 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - April 1, 2015 Category: Urology & Nephrology Authors: Kitterer D, Latus J, Ulmer C, Fritz P, Biegger D, Ott G, Alscher MD, Witowski J, Kawka E, Joerres A, Seeger H, Segerer S, Braun N Tags: Am J Physiol Renal Physiol Source Type: research

Memories that Last in Hypertension.
Abstract In recent years it has become clear that the immune system contributes to the genesis of hypertension. Hypertensive stimuli such as angiotensin II, DOCA-salt, norepinephrine cause T cells and monocytes/macrophages to accumulate in the kidney and vasculature. These cells release inflammatory cytokines such as IL-6, IFN γ, and IL-17 that promote renal and vascular dysfunction. These cytokines also promote angiotensinogen production in the proximal tubule and sodium retention in the distal nephron and contribute to renal fibrosis and glomerular damage. For several years, we have observed accumulation o...
Source: Am J Physiol Renal P... - April 1, 2015 Category: Urology & Nephrology Authors: Itani HA, Harrison DG Tags: Am J Physiol Renal Physiol Source Type: research

KCNJ10 (Kir4.1) is expressed in the basolateral membrane of the cortical thick ascending limb.
Abstract The aim of the present study is to examine the role of Kcnj10 (Kir.4.1) in contributing to the basolateral K conductance in the cortical thick ascending limb (cTAL) using Kcnj10+/+ (WT) and Kcnj10-/- (KO) mice. The patch-clamp experiments detected a 40 pS and an 80 pS K channel in the basolateral membrane of the cTAL. Moreover, the probability of finding the 40 pS K was significantly higher in the top half of the cTAL than those in the low half. Immunostaining showed that Kcnj10 staining was detected in the basolateral membrane of the cTAL but the expression was not uniformly distributed. The disruption o...
Source: Am J Physiol Renal P... - April 1, 2015 Category: Urology & Nephrology Authors: Zhang C, Wang L, Su XT, Lin DH, Wang WH Tags: Am J Physiol Renal Physiol Source Type: research

Corrigendum.
Authors: PMID: 25834130 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - April 1, 2015 Category: Urology & Nephrology Tags: Am J Physiol Renal Physiol Source Type: research

Am J Physiol Renal Physiol; +19 new citations
19 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Physiol Renal Physiol These pubmed results were generated on 2015/03/29PubMed comprises more than 24 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites. (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - March 29, 2015 Category: Urology & Nephrology Tags: Report Source Type: research

Impairment of HNF4α Binding to stim1 Promoter Contributes to High Glucose-induced Upregulation of STIM1 Expression in Glomerular Mesangial Cells.
Impairment of HNF4α Binding to stim1 Promoter Contributes to High Glucose-induced Upregulation of STIM1 Expression in Glomerular Mesangial Cells. Am J Physiol Renal Physiol. 2015 Mar 18;:ajprenal.00563.2014 Authors: Wang Y, Chaudhari S, Ren Y, Ma R Abstract The present study was carried out to investigate if hepatic nuclear factor 4α (HNF4α) contributed to high glucose-induced increase in STIM1 protein abundance in glomerular mesangial cells (MCs). Western blot and immunofluorescence studies showed HNF4α expression in MCs. Knockdown of HNF4α using siRNA approach significa...
Source: Am J Physiol Renal P... - March 18, 2015 Category: Urology & Nephrology Authors: Wang Y, Chaudhari S, Ren Y, Ma R Tags: Am J Physiol Renal Physiol Source Type: research

Am J Physiol Renal Physiol; +24 new citations
24 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Physiol Renal Physiol These pubmed results were generated on 2015/03/13PubMed comprises more than 24 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites. (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - March 14, 2015 Category: Urology & Nephrology Tags: Report Source Type: research

IR- Interleukin-22 in kidney injury and regeneration.
Abstract Interleukins have become well known regulators of innate and adaptive immunity-related tissue inflammation. Recently, IL-22 has gained a lot of interest for its unique functions in maintaining and regaining epithelial integrity. IL-22 is exclusively secreted by different immune cell subsets, while IL-22 receptors are mainly expressed by epithelial cells. As the kidney is largely an epithelial organ the functional role of IL-22 in the kidney deserves to be explored in detail. Here we briefly summarize the key features of IL-22 biology and review the available data on its expression and functional roles in ...
Source: Am J Physiol Renal P... - March 4, 2015 Category: Urology & Nephrology Authors: Weidenbusch M, Rodler S, Anders HJ Tags: Am J Physiol Renal Physiol Source Type: research

N-Acetyl-Seryl-Aspartyl-Lysyl-Proline: mechanisms of renal protection in mouse model of systemic lupus erythematosus.
Abstract Systemic lupus erythematosus is an autoimmune disease characterized by the development of auto antibodies against a variety of self-antigens and deposition of immune complexes that lead to inflammation, fibrosis and end organ damage. Up to 60% of lupus patients develop nephritis and renal dysfunction leading to kidney failure. N-acetyl-seryl-aspartyl-lysyl-proline, i.e. Ac-SDKP, is a natural tetrapeptide that in hypertension prevents inflammation and fibrosis in heart, kidney, and vasculature. In experimental autoimmune myocarditis Ac-SDKP prevents cardiac dysfunction by decreasing innate and adaptive imm...
Source: Am J Physiol Renal P... - March 4, 2015 Category: Urology & Nephrology Authors: Liao TD, Nakagawa P, Janic B, D'Ambrosio MA, Worou ME, Peterson EL, Rhaleb NE, Yang XP, Carretero OA Tags: Am J Physiol Renal Physiol Source Type: research

mTOR inhibition with temsirolimus causes acute increases in glomerular permeability, but inhibits the dynamic permeability actions of puromycin aminonucleoside.
Abstract Inhibitors of the mammalian target of rapamycin (mTORi) can produce de novo proteinuria in kidney transplant patients. On the other hand, mTORi has been shown to suppress disease progression in several animal models of kidney disease. In the present study we investigated whether glomerular permeability can be acutely altered by the mTORi, temsirolimus, and whether mTORi can affect acute purumycin aminonucleoside (PAN) or angiotensin II (AngII) induced glomerular hyperpermeability. In anaesthetized Wistar rats, the left ureter was cannulated for urine collection, while simultaneously, blood access was achi...
Source: Am J Physiol Renal P... - March 4, 2015 Category: Urology & Nephrology Authors: Axelsson J, Rippe A, Rippe B Tags: Am J Physiol Renal Physiol Source Type: research

Am J Physiol Renal Physiol; +27 new citations
27 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Physiol Renal Physiol These pubmed results were generated on 2015/02/14PubMed comprises more than 24 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites. (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - February 14, 2015 Category: Urology & Nephrology Tags: Report Source Type: research

Am J Physiol Renal Physiol; +22 new citations
22 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Physiol Renal Physiol These pubmed results were generated on 2015/02/07PubMed comprises more than 24 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites. (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - February 7, 2015 Category: Urology & Nephrology Tags: Report Source Type: research

Am J Physiol Renal Physiol; +19 new citations
19 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Physiol Renal Physiol These pubmed results were generated on 2015/01/24PubMed comprises more than 24 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites. (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - January 24, 2015 Category: Urology & Nephrology Tags: Report Source Type: research

Arterial stiffness : an independent determinant of adaptive glomerular hyperfiltration after kidney donation.
Abstract After kidney donation, the remaining kidney tends to hyperfiltrate thus limiting the initial loss of renal function. The potential determinants of this adaptive glomerular hyperfiltration (GHF) and specifically the influence of arterial function are poorly known. In 45 normotensive healthy kidney donors (51 +/- 10 yrs [mean+/-SD], 39 females), glomerular filtration rate (GFR) was measured as the clearance of continuously infused (99m)Tc-DTPA and timed urine collections at baseline -i.e. before donation- and 1 year after donation. GHF was computed as post-donation GFR minus half of baseline GFR. Arterial f...
Source: Am J Physiol Renal P... - January 7, 2015 Category: Urology & Nephrology Authors: Fesler P, Mourad G, du Cailar G, Ribstein J, Mimran A Tags: Am J Physiol Renal Physiol Source Type: research

An Angiotensin-(1-7) Endopeptidase in the Kidney Cortex, Proximal Tubules and Human HK-2 Epithelial Cells that is Distinct from Insulin Degrading Enzyme.
Abstract Angiotensin 1-7 (Ang-(1-7)) is expressed within the kidney and exhibits renoprotective actions that antagonizes the inflammatory, fibrotic and pro-oxidant effects of Ang II. We previously identified an endopeptidase that preferentially metabolized Ang-(1-7) to Ang-(1-4) in the brain medulla and cerebrospinal fluid (CSF) of sheep; thus, the present study established the expression of the peptidase in the kidney. Utilizing a sensitive HPLC-based approach, we demonstrate an endopeptidase activity that hydrolyzed Ang-(1-7) to Ang-(1-4) in the sheep cortex, isolated tubules and human HK-2 renal epithelial cell...
Source: Am J Physiol Renal P... - January 7, 2015 Category: Urology & Nephrology Authors: Wilson BA, Cruz-Diaz N, Marshall AC, Pirro NT, Su Y, Gwathmey TM, Rose JC, Chappell MC Tags: Am J Physiol Renal Physiol Source Type: research

Am J Physiol Renal Physiol; +17 new citations
17 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Physiol Renal Physiol These pubmed results were generated on 2015/01/01PubMed, a service of the National Library of Medicine, includes over 15 million citations for biomedical articles back to the 1950's. These citations are from MEDLINE and additional life science journals. PubMed includes links to many sites providing full text articles and other related resources. (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - January 1, 2015 Category: Urology & Nephrology Tags: Report Source Type: research

Am J Physiol Renal Physiol; +32 new citations
32 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Physiol Renal Physiol These pubmed results were generated on 2014/12/20PubMed, a service of the National Library of Medicine, includes over 15 million citations for biomedical articles back to the 1950's. These citations are from MEDLINE and additional life science journals. PubMed includes links to many sites providing full text articles and other related resources. (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - December 21, 2014 Category: Urology & Nephrology Tags: Report Source Type: research

Renal Human Organic Anion Transporter 3 increases the Susceptibility of Lymphoma Cells to Bendamustine Uptake.
We examined the effects of structural analogs of p-aminohippurate, melphalan, chlorambucil and bendamustine, on OAT1-mediated [(3)H]PAH uptake, and OAT3- and OAT4- mediated [(3)H]estrone sulfate uptake in stably-transfected HEK293 cells. Melphalan had no significant inhibitory effect on any OAT whereas chlorambucil reduced OAT1-, OAT3- and OAT4-mediated uptake of PAH or estrone sulfate down to 14.6%, 16.3% and 66.0% of control, respectively. Bendamustine inhibited only the OAT3-mediated estrone sulfate uptake, which was reduced down to 14.3% of control cells, suggesting that it interacts exclusively with OAT3. The IC50 val...
Source: Am J Physiol Renal P... - December 4, 2014 Category: Urology & Nephrology Authors: Hagos Y, Hundertmark P, Shnitsar V, Marada VV, Wulf G, Burckhardt G Tags: Am J Physiol Renal Physiol Source Type: research

STE20/SPS1-related Proline/alanine-rich Kinase (SPAK) is Critical for Sodium Reabsorption in Isolated Perfused Thick Ascending Limb.
In conclusion, the combined net effect of SPAK isoforms on sodium reabsorption in TAL is stimulatory. SPAK is not essential for vasopressin stimulation of sodium reabsorption in TAL. PMID: 25477470 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - December 4, 2014 Category: Urology & Nephrology Authors: Cheng CJ, Yoon J, Baum M, Huang CL Tags: Am J Physiol Renal Physiol Source Type: research

Cyclic stretch induced TGF-β1 and fibronectin expression is mediated by β1 integrin through c-Src- and STAT3- dependent pathways in renal epithelial cells.
In this study, we investigated the mechanism by which mechanical stretch, an in vitro model for chronic obstructive nephropathy, regulates fibronectin and transforming growth factor-β1 (TGF-β1) expression in cultured human proximal tubular epithelium (HK-2) cells. Mechanical stretch up-regulated fibronectin and TGF-β1 expression and activated STAT3 in a time-dependent manner. Stretch-induced fibronectin and TGF-β1 were suppressed by a STAT3 inhibitor, S3I-201 and by small interfering RNA (siRNA) targeting human STAT3 (STAT3 siRNA). Similarly, fibronectin and TGF-β1 expression and STAT3 activation i...
Source: Am J Physiol Renal P... - December 4, 2014 Category: Urology & Nephrology Authors: Hamzeh MT, Sridhara R, Alexander LD Tags: Am J Physiol Renal Physiol Source Type: research

Hemodynamic basis for the limited renal injury in rats with Angiotensin II-induced hypertension.
Abstract ANG II is thought to increase the susceptibility to hypertension-induced renal disease (HIRD) via BP-dependent and BP-independent pathways; however, the quantitative relationships between BP and HIRD have not been examined in ANG II-infused hypertensive rats. We compared the relationship between radiotelemetrically-measured BP and HIRD in Sprague-Dawley rats (Harlan) chronically administered ANG II (300-500 ng/kg/min, n=19) for 4 weeks vs. another commonly employed pharmacologic model of hypertension induced by the chronic administration of N(ω)-nitro-L-arginine methyl ester (L-NAME, 50 mg/kg/day, n...
Source: Am J Physiol Renal P... - December 4, 2014 Category: Urology & Nephrology Authors: Polichnowski AJ, Griffin KA, Picken MM, Licea-Vargas H, Long J, Williamson GA, Bidani AK Tags: Am J Physiol Renal Physiol Source Type: research

Generation of WNK1 knockout cell lines by CRISPR/Cas-mediated genome editing.
Abstract Sodium-coupled SLC12 cation chloride cotransporters play important roles in cell volume and chloride homeostasis, epithelial fluid secretion, and renal tubular salt reabsorption. These cotransporters are phosphorylated and activated indirectly by With-No-Lysine (WNK) kinases through their downstream effector kinases, SPAK and OSR1. Multiple WNK kinases can coexist within a single cell type, although their relative contributions to SPAK/OSR1 activation and salt transport remain incompletely understood. Deletion of specific WNKs from cells that natively express a functional WNK-SPAK/OSR1 network will help r...
Source: Am J Physiol Renal P... - December 4, 2014 Category: Urology & Nephrology Authors: Roy A, Goodman JH, Begum G, Donnelly BF, Pittman G, Weinman EJ, Sun D, Subramanya AR Tags: Am J Physiol Renal Physiol Source Type: research

Theobromine increases NAD+/Sirt1 activity and protects the kidney under diabetic conditions.
Abstract Reduction in sirtuin 1 (Sirt-1) is associated with extracellular matrix (ECM) accumulation in the diabetic kidney. Theobromine may reduce kidney ECM accumulation in diabetic rats. In the current study, we aimed to unravel, under diabetic conditions, the mechanism of kidney ECM accumulation induced by a reduction in Sirt-1 and the effect of theobromine in these events. In vitro, we used immortalized human mesangial cells (iHMCs) exposed to high glucose (HG, 30 mM), with or without small interfering RNA for NOX4 and Sirt1. In vivo, spontaneously hypertensive rats (SHR) were rendered diabetic by means of str...
Source: Am J Physiol Renal P... - November 19, 2014 Category: Urology & Nephrology Authors: Papadimitriou A, Silva KC, Peixoto EB, Borges CM, Lopes de Faria JM, Lopes de Faria JB Tags: Am J Physiol Renal Physiol Source Type: research

Renalase regulates peripheral and central dopaminergic activities.
RENALASE REGULATES PERIPHERAL AND CENTRAL DOPAMINERGIC ACTIVITIES. Am J Physiol Renal Physiol. 2014 Nov 19;:ajprenal.00274.2014 Authors: Quelhas-Santos J, Serrão MP, Soares-Silva I, Fernandes-Cerqueira C, Simões-Silva L, Pinho MJ, Remião F, Sampaio-Maia B, Desir GV, Pestana M Abstract Renalase is a recently identified FAD/NADH-dependent amine oxidase mainly expressed in kidney, which is secreted into blood and urine where it was suggested to metabolize catecholamines. The present study evaluated central and peripheral dopaminergic activities in the renalase knockout (KO) mouse mod...
Source: Am J Physiol Renal P... - November 19, 2014 Category: Urology & Nephrology Authors: Quelhas-Santos J, Serrão MP, Soares-Silva I, Fernandes-Cerqueira C, Simões-Silva L, Pinho MJ, Remião F, Sampaio-Maia B, Desir GV, Pestana M Tags: Am J Physiol Renal Physiol Source Type: research