Renal PKC epsilon deficiency attenuates acute kidney injury and ischemic allograft injury via TNF alpha dependent inhibition of apoptosis and inflammation.
Abstract Acute kidney injury (AKI) increases the risk of morbidity and mortality after major surgery and transplantation. We investigated the effect of protein kinase C (PKC) epsilon deficiency on AKI and ischemic allograft damage after kidney transplantation (ktx). PKC epsilon deficient and wild type (WT) control mice were subjected to 35 minutes of renal pedicle clamping to induced AKI. PKC epsilon deficiency was associated with a marked improvement in survival and an attenuated loss of kidney function. Furthermore, functional MRI studies revealed better renal perfusion in PKC epsilon deficient than WT mice one ...
Source: Am J Physiol Renal P... - July 23, 2014 Category: Urology & Nephrology Authors: Rong S, Hueper K, Kirsch T, Greite R, Klemann C, Mengel M, Meier M, Menne J, Leitges M, Susnik N, Meier M, Haller H, Shushakova N, Gueler F Tags: Am J Physiol Renal Physiol Source Type: research

Extra-renal citrulline disposal in mice with impaired renal function.
EXTRA-RENAL CITRULLINE DISPOSAL IN MICE WITH IMPAIRED RENAL FUNCTION. Am J Physiol Renal Physiol. 2014 Jul 23; Authors: Marini JC, Cajo Didelija I, Fiorotto ML Abstract The endogenous synthesis of arginine, a semi-essential amino acid, relies on the production of citrulline by the gut and its conversion into arginine by the kidney in what has been called the 'intestinal-renal axis' for arginine synthesis. Although the kidney is the main site for citrulline disposal, it only accounts for ~60-70% of the citrulline produced. Because the only known fate for citrulline is arginine synthesis and the enzymes...
Source: Am J Physiol Renal P... - July 23, 2014 Category: Urology & Nephrology Authors: Marini JC, Cajo Didelija I, Fiorotto ML Tags: Am J Physiol Renal Physiol Source Type: research

Inhibition of smooth muscle force generation by focal adhesion kinase (FAK) inhibitors in the hyperplastic human prostate.
In conclusion, α1-adrenergic smooth muscle contraction or its regulation involves FAK in the human prostate. Consequently, FAK may be involved in pathophysiology of LUTS, and in current or future LUTS therapies. PMID: 25056351 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - July 23, 2014 Category: Urology & Nephrology Authors: Kunit T, Gratzke C, Schreiber A, Strittmatter F, Waidelich R, Rutz B, Loidl W, Andersson KE, Stief CG, Hennenberg M Tags: Am J Physiol Renal Physiol Source Type: research

Somatic Modulation of Spinal Reflex Bladder Activity Mediated by Nociceptive Bladder Afferent Nerve Fibers in Cats.
Abstract The goal of this study was to determine if supraspinal pathways are necessary for inhibition of bladder reflex activity induced by activation of somatic afferents in the pudendal or tibial nerve. Cats anesthetized with α-chloralose were studied after acute spinal cord transection at the thoracic T9/T10 level. Dilute (0.25%) acetic acid (AA) was used to irritate bladder, activate nociceptive afferent C-fibers and trigger spinal reflex bladder contractions (amplitude 19.3±2.9 cmH2O). Hexamethonium (a ganglionic blocker, i.v.) significantly (p
Source: Am J Physiol Renal P... - July 23, 2014 Category: Urology & Nephrology Authors: Xiao Z, Rogers MJ, Shen B, Wang J, Schwen Z, Roppolo JR, de Groat WC, Tai C Tags: Am J Physiol Renal Physiol Source Type: research

Nanotechnology and Adeno-associated virus based decorin gene therapy ameliorates Peritoneal Fibrosis.
Conclusion: This study shows that both GNP-delivered and AAV-mediated decorin gene therapies significantly decrease peritoneal fibrosis in vivo in rodent model. This approach has important clinical translational potential in providing a therapeutic strategy to prevent peritoneal fibrosis in PD patients. PMID: 25056353 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - July 23, 2014 Category: Urology & Nephrology Authors: Chaudhary K, Moore H, Tandon A, Gupta S, Khanna R, Mohan RR Tags: Am J Physiol Renal Physiol Source Type: research

A Knowledge Base of Vasopressin Actions in Kidney.
Abstract Biological information is growing at a rapid pace, making it difficult for individual investigators to be familiar with all information that is relevant to their own research. Computers are beginning to be used to extract and curate biological information; however, the complexity of human language used in research papers continues to be a critical barrier to full automation of knowledge extraction. Here, we report a manually curated knowledge base of vasopressin actions in renal epithelial cells that is designed to be readable either by humans or by computer programs using natural language processing algo...
Source: Am J Physiol Renal P... - July 23, 2014 Category: Urology & Nephrology Authors: Sanghi A, Zaringhalam M, Corcoran CC, Saeed F, Hoffert JD, Sandoval PC, Pisitkun T, Knepper MA Tags: Am J Physiol Renal Physiol Source Type: research

Renal MODY-fier Genes.
Abstract Editorial Focus - F -422-2013. PMID: 25056355 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - July 23, 2014 Category: Urology & Nephrology Authors: Calvet JP Tags: Am J Physiol Renal Physiol Source Type: research

Mechanism of impaired afferent arteriole myogenic response in Dahl salt sensitive rats: role of 20-HETE.
Abstract The afferent arteriole (Af-Art) controls glomerular capillary pressure, an important determinant of glomerular injury. Af-Art myogenic response is mediated by ATP, and ATP signaling is in turn mediated by 20-HETE. Dahl salt-sensitive rats (Dahl SS) have decreased renal 20-HETE production. We hypothesized that Dahl SS have an impaired myogenic response and constrictor response to ATP, due to decreased 20-HETE. Af-Arts from Dahl SS or Dahl salt resistant rats (Dahl SR) were microdissected and perfused. When myogenic response was induced by increasing Af-Art perfusion pressure from 60 to 140 mmHg, luminal Af...
Source: Am J Physiol Renal P... - July 2, 2014 Category: Urology & Nephrology Authors: Ren Y, D'Ambrosio MA, Garvin JL, Peterson EL, Carretero OA Tags: Am J Physiol Renal Physiol Source Type: research

Renal cortical hexokinase and pentose phosphate pathway activation through the EGFR/Akt signaling pathway in endotoxin-induced acute kidney injury.
Abstract While disruption of energy production is an important contributor to renal injury, metabolic alterations in sepsis-induced AKI remain understudied. We assessed changes in renal cortical glycolytic metabolism in a mouse model of sepsis-induced AKI. A specific and rapid increase in hexokinase (HK) activity (~2 fold) was observed 3 h after lipopolysaccharide (LPS) exposure and maintained up to 18 h, in association with a decline in renal function as measured by BUN. LPS-induced hexokinase activation occurred independently of hexokinase isoform expression or mitochondrial localization. No other changes in gly...
Source: Am J Physiol Renal P... - July 2, 2014 Category: Urology & Nephrology Authors: Smith JA, Stallons LJ, Schnellmann RG Tags: Am J Physiol Renal Physiol Source Type: research

Mammalian target of rapamycin complex 1 activation in podocytes promotes cellular crescent formation.
Abstract Abstract Podocytes play a key role in the formation of cellular crescents in experimental and human diseases. However, the underlying mechanisms for podocytes in promoting crescent formation need further investigation. Here we demonstrated that mammalian target of rapamycin complex 1 (mTORC1) signaling was remarkably activated and HIF1alpha expression was largely induced in cellular crescents from patients with crescentic glomerular diseases. Specific deletion of Tsc1 in podocytes led to mTORC1 activation in podocytes and kidney dysfunction in mice. Interestingly, 33 of 36 knockouts developed cellular or ...
Source: Am J Physiol Renal P... - July 2, 2014 Category: Urology & Nephrology Authors: Mao J, Zeng Z, Xu Z, Li J, Jiang L, Fang Y, Xu X, Hu Z, He W, Yang J, Dai C Tags: Am J Physiol Renal Physiol Source Type: research

Do Large Pores in the Glomerular Capillary Wall Account for Albuminuria in Nephrotic States?
In this study we test for large pore formation in a novel manner. We account for the rates of plasma elimination as determined for tritium labeled tracers of uncharged polydisperse Ficoll (radii range 35-85 Å) and two globular 14C-labelled proteins, albumin (radius 36 Å) and IgG (radius 55 Å), in control and puromycin aminonucleoside nephrotic (PAN) Sprague-Dawley rats. The plasma elimination rates were then matched to the urinary excretion of these labelled materials (n=7). Albumin and IgG plasma retention rates were identical and far enhanced as compared to the retention rates of inert transport markers...
Source: Am J Physiol Renal P... - July 2, 2014 Category: Urology & Nephrology Authors: Vuchkova J, Koltun M, Greive K, Comper WD Tags: Am J Physiol Renal Physiol Source Type: research

Long-term recording of external urethral sphincter EMG activity in unanesthetized unrestrained rats.
We describe a method for long-term recording of EUS EMG by implantation of fine wires adjacent to the EUS that are secured to the pubic bone. Wires pass subcutaneously to a skull-mounted plug and connect to the recording apparatus by a flexible cable attached to a commutator. A force transducer-mounted cup under a metabolic cage collected urine, allowing recording of EUS EMG and voided urine weight without anesthesia or restraint. Implant durability permitted EUS EMG recording during repeated (up to 3x weekly) 24-hour sessions for more than eight weeks. EMG and voiding properties were stable over weeks 2-8. The degree of E...
Source: Am J Physiol Renal P... - July 2, 2014 Category: Urology & Nephrology Authors: LaPallo BK, Wolpaw JR, Chen XY, Carp JS Tags: Am J Physiol Renal Physiol Source Type: research

REGULATION OF (PRO)RENIN RECEPTOR EXPRESSION IN mIMCD VIA GSK3β-NFAT5- SIRT-1 SIGNALING PATHWAY.
REGULATION OF (PRO)RENIN RECEPTOR EXPRESSION IN mIMCD VIA GSK3β-NFAT5- SIRT-1 SIGNALING PATHWAY. Am J Physiol Renal Physiol. 2014 Jul 2; Authors: Quadri S, Siragy HM Abstract The localization and regulation of (Pro)renin receptor expression (PRR) in the kidney collecting duct cells is not well established. We hypothesized that low salt contributes to the regulation of PRR expression in these cells via GSK3β-NFAT5-SIRT-1 signaling pathway. mIMCD were treated with NaCl at 130mM (NS), 63mM (LS) or 209mM (HS) alone or in combination with NFAT5 scrambled siRNA, NFAT5 siRNA, or the SIRT-1 inhibito...
Source: Am J Physiol Renal P... - July 2, 2014 Category: Urology & Nephrology Authors: Quadri S, Siragy HM Tags: Am J Physiol Renal Physiol Source Type: research

Leishmania infantum-chagasi activates SHP-1 and reduces NFAT5/TonEBP activity in the mouse kidney inner medulla.
Abstract Visceral leishmaniasis patients have been reported to have a urine concentration defect. Concentration of urine by the renal inner medulla is essentially dependent on a transcription factor, NFAT5/TonEBP, because it activates expression of osmoprotective genes BGT1 and SMIT, and water channel aquaporin-2, all of which are imperative for concentrating urine. Leishmania parasites evade macrophage immune defenses by activating protein tyrosine phosphatases, among which SHP-1 is critical. We previously demonstrated that SHP-1 inhibits tonicity-dependent activation of NFAT5/TonEBP in HEK293 cells through scree...
Source: Am J Physiol Renal P... - July 2, 2014 Category: Urology & Nephrology Authors: Zhou X, Wang H, Koles NL, Zhang A, Aronson NE Tags: Am J Physiol Renal Physiol Source Type: research

Myeloperoxidase deficiency ameliorates progression of chronic kidney disease in mice.
Conclusion: These results demonstrate that MPO deficiency ameliorates renal injury in the renal ablation model of CKD in mice. PMID: 24990898 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - July 2, 2014 Category: Urology & Nephrology Authors: Lehners A, Lange S, Niemann G, Rosendahl A, Meyer-Schwesinger C, Oh J, Stahl RA, Ehmke H, Benndorf R, Klinke A, Baldus S, Wenzel U Tags: Am J Physiol Renal Physiol Source Type: research

Interactive Roles of CD73 and Tissue Non-Specific Alkaline Phosphatase in the Renal Vascular Metabolism of 5'-AMP.
Conclusion: CD73 and TNAP play interactive roles to metabolize luminally applied 5'-AMP in the renal vasculature such that inhibition of both is required to inhibit the production of adenosine. PMID: 24990899 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - July 2, 2014 Category: Urology & Nephrology Authors: Jackson EK, Cheng D, Verrier JD, Janesko-Feldman K, Kochanek PM Tags: Am J Physiol Renal Physiol Source Type: research

Insights into Acidosis-induced Regulation of SLC26A4 (pendrin) and SLC4A9 (AE4) Transporters Using 3-Dimensional Morphometric Analysis of β-Intercalated Cells.
Conclusion: Acidosis induces down-regulation of anion exchanger expression in β-ICs and diminished presence of pendrin in apical recycling endosomes. PMID: 24990900 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - July 2, 2014 Category: Urology & Nephrology Authors: Purkerson JM, Heintz EV, Nakamori A, Schwartz GJ Tags: Am J Physiol Renal Physiol Source Type: research

Voluntary wheel running augments aortic l-arginine transport and endothelial function in rats with chronic kidney disease.
VOLUNTARY WHEEL RUNNING AUGMENTS AORTIC L-ARGININE TRANSPORT AND ENDOTHELIAL FUNCTION IN RATS WITH CHRONIC KIDNEY DISEASE. Am J Physiol Renal Physiol. 2014 Jun 25; Authors: Martens CR, Kuczmarski JM, Kim J, Guers JJ, Harris MB, Lennon-Edwards S, Edwards DG Abstract Reduced nitric oxide (NO) synthesis contributes to risk for cardiovascular disease in chronic kidney disease (CKD). Vascular uptake of the NO precursor L-arginine (ARG) is attenuated in rodents with CKD, resulting in reduced substrate availability for NO synthesis and impaired vascular function. We tested the effect of 4 weeks of voluntary ...
Source: Am J Physiol Renal P... - June 25, 2014 Category: Urology & Nephrology Authors: Martens CR, Kuczmarski JM, Kim J, Guers JJ, Harris MB, Lennon-Edwards S, Edwards DG Tags: Am J Physiol Renal Physiol Source Type: research

Hierarchy of molecules in tgf-β1 signaling relevant to myofibroblast activation and renal fibrosis.
HIERARCHY OF MOLECULES IN TGF-β1 SIGNALING RELEVANT TO MYOFIBROBLAST ACTIVATION AND RENAL FIBROSIS. Am J Physiol Renal Physiol. 2014 Jun 25; Authors: Zhan M, Kanwar YS Abstract editorial focus- 546-2013. PMID: 24966086 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - June 25, 2014 Category: Urology & Nephrology Authors: Zhan M, Kanwar YS Tags: Am J Physiol Renal Physiol Source Type: research

Chronic kidney disease: Targeting prostaglandin E2 receptors.
Abstract Chronic kidney disease is a leading cause of morbidity and mortality in the world. A better understanding of disease mechanisms has been gained in recent years, but the current management strategies are ineffective at preventing disease progression. A widespread focus of research is placed on elucidating the specific processes implicated to find more effective therapeutic options. Prostaglandin E2 acting on its four EP receptors regulates many renal disease processes, thus EP receptors could prove to be important targets for kidney disease intervention strategies. This review summarizes the major pathogen...
Source: Am J Physiol Renal P... - June 25, 2014 Category: Urology & Nephrology Authors: Nasrallah R, Hassouneh R, Hebert RL Tags: Am J Physiol Renal Physiol Source Type: research

Aldosterone Sensitizes Connecting Tubule Glomerular Feedback via the Aldosterone Receptor GPR30.
Abstract Increasing Na delivery to epithelial Na channels (ENaC) in the connecting tubule (CNT) dilates the afferent arteriole (Af-Art), a process we call connecting tubule glomerular feedback (CTGF). We hypothesize that aldosterone sensitizes CTGF via a nongenomic mechanism that stimulates CNT ENaC via the aldosterone receptor GPR30. Rabbit Af-Arts and their adherent CNTs were microdissected and simultaneously perfused. Two consecutive CTGF curves were elicited by increasing luminal NaCl in the CNT. During the control period, the concentration of NaCl that elicited a half-maximal response (EC50) was 37.0±2...
Source: Am J Physiol Renal P... - June 25, 2014 Category: Urology & Nephrology Authors: Ren Y, D'Ambrosio MA, Garvin JL, Leung P, Kutskill K, Wang H, Peterson EL, Carretero OA Tags: Am J Physiol Renal Physiol Source Type: research

Cyp2c44-epoxygenase in the collecting duct (CD) is essential for high potassium (K) intake-induced antihypertensive effect.
Abstract The P450 Cyp2c44 epoxygenase metabolizes arachidonic acid (AA) to epoxyeicosatrienoic acids (EETs) in kidney and vascular tissues. We now used real time quantitative PCR techniques (qPCR) to examine the effect of high salt or high potassium (HK) intake on the expression of Cyp2c44, a major Cyp2c epoxygenase in the mouse kidney. We detected Cyp2c44 in proximal convoluted tubule (PCT), thick ascending limb (TAL), distal convoluted tubule (DCT)/connecting tubule (CNT), and collecting duct (CD). A high salt diet increased the expression of Cyp2c44 in the TAL and DCT/CNT, but not in the PCT and CD. In contrast...
Source: Am J Physiol Renal P... - June 25, 2014 Category: Urology & Nephrology Authors: Wang WH, Zhang C, Lin D, Wang L, Graves JP, Zeldin DC, Capdevila JH Tags: Am J Physiol Renal Physiol Source Type: research

TRPV4 activation mediates flow-induced nitric oxide production in the rat thick ascending limb.
Abstract Nitric oxide (NO) regulates renal function. Luminal flow stimulates NO production in the thick ascending limb (TAL). Transient Receptor Potential Vanilloid 4 (TRPV4) is a mechano-sensitive channel activated by luminal flow in different types of cells. We hypothesized that TRPV4 mediates flow-induced NO production in the rat TAL. We measured NO production in isolated, perfused rat TALs using the fluorescent dye DAF FM. Increasing luminal flow from 0 to 20 nL/min stimulated NO from 8±3 to 45±12 arbitrary units (AU)/min (n=5;p
Source: Am J Physiol Renal P... - June 25, 2014 Category: Urology & Nephrology Authors: Cabral PD, Garvin JL Tags: Am J Physiol Renal Physiol Source Type: research

Differential effect of T-type voltage-gated calcium channel disruption on renal plasma flow and glomerular filtration rate in vivo.
In conclusion, Cav 3.2 channels support dilatation of efferent arterioles and affect GFR while Cav 3.1 channels in vivo contribute to renal vascular resistance. It is suggested that endothelial and nerve localization of Cav 3.2 and Cav 3.1, respectively, may account for the observed effects. PMID: 24966091 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - June 25, 2014 Category: Urology & Nephrology Authors: Thuesen AD, Andersen H, Cardel M, Toft A, Walter S, Marcussen N, Jensen BL, Bie P, Hansen PB Tags: Am J Physiol Renal Physiol Source Type: research

Am J Physiol Renal Physiol; +18 new citations
18 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Physiol Renal Physiol These pubmed results were generated on 2014/06/24PubMed, a service of the National Library of Medicine, includes over 15 million citations for biomedical articles back to the 1950's. These citations are from MEDLINE and additional life science journals. PubMed includes links to many sites providing full text articles and other related resources. (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - June 24, 2014 Category: Urology & Nephrology Tags: Report Source Type: research

Blockade of Cysteine-rich Protein 61 Attenuates Renal Inflammation and Fibrosis after Ischemic Kidney Injury.
This study aimed to investigate the role of Cysteine-rich protein 61 (Cyr61) after unilateral kidney ischemia-reperfusion injury (IRI) in mice. After IRI, increased expression of Cyr61 was detected, predominately in the proximal tubular epithelium. This was confirmed by in vitro experiments, which showed that hypoxia stimulates Cyr61 expression in cultured proximal tubular epithelial cells. The proinflammatory property of Cyr61 was indicated by its ability to upregulate monocyte chemoattractant protein (MCP)-1 and interleukin (IL)-6. Additionally, we found elevated urinary Cyr61 excretion in patients with AKI. Notably, tre...
Source: Am J Physiol Renal P... - June 11, 2014 Category: Urology & Nephrology Authors: Lai CF, Lin SL, Chiang WC, Chen YM, Wu VC, Young GH, Ko WJ, Kuo ML, Tsai TJ, Wu KD Tags: Am J Physiol Renal Physiol Source Type: research

WNK3 abrogates the NEDD4-2-mediated inhibition of the renal Na+:Cl- Cotransporter.
Abstract The serine/threonine kinase WNK3 and the ubiquitin-protein ligase NEDD4-2 are key regulators of the thiazide-sensitive Na(+):Cl- cotransporter (NCC), WNK3 as an activator and NEDD4-2 as an inhibitor. Nedd4-2 was identified as an interacting partner of WNK3 through a GST pulldown assay using the N-terminal domain of WNK3, combined with LC-MS/MS analysis. This was validated by co-immunoprecipitation of WNK3 and NEDD4-2 expressed in HEK293 cells. Our data also revealed that the interaction between Nedd4-2 and WNK3 does not involve the PY-like motif found in WNK3. The level of WNK3 ubiquitylation did not chan...
Source: Am J Physiol Renal P... - June 11, 2014 Category: Urology & Nephrology Authors: Lagnaz D, Arroyo JP, Chavez-Canales M, Vazquez N, Rizzo F, Spirli A, Debonneville A, Staub O, Gamba G Tags: Am J Physiol Renal Physiol Source Type: research

Angiotensin II stimualtes fibronectin protein synthesis via a G βγ⁄arachidonic acid-dependent pathway.
In this study, we determined the effects of Ang II on fibronectin expression in cultured rabbit proximal tubule cells and elucidted the signaling pathways associated with such expression. We found that Ang II and transfection of Gβγ subunits directly increased fibronectin protein expression, and this increase was inhibited by overexpression of βARK-ct or a DN-Src. Moreover, Ang II-induced fibronectin protein expression was significantly abrogated by the AT2 receptor antagonist PD123319. In addition, inhibition of cPLA2) diminished Ang II-induced fibronectin expression. Endogenous arachidonic acid mimicked A...
Source: Am J Physiol Renal P... - June 11, 2014 Category: Urology & Nephrology Authors: Alexander LD, Ding Y, Alagarsamy S, Cui X Tags: Am J Physiol Renal Physiol Source Type: research

Evidence for pericyte origin of TSC-associated renal angiomyolipomas and implications for angiotensin receptor inhibition therapy.
Abstract Nearly all patients with tuberous sclerosis complex (TSC) develop renal angiomyolipomas, although the tumor cell of origin is unknown. We observed decreased renal angiomyolipoma development in patients with the TSC2-PKD1 deletion syndrome and hypertension that were treated from an early age with angiotensin converting enzyme inhibitors or angiotensin receptor blockers. TSC-associated renal angiomyolipomas expressed angiotensin II type 1 receptor, platelet derived growth factor receptor β and VEGFR2, but did not express the adipocyte marker S100 or the endothelial marker CD31. Sera of TSC patients exh...
Source: Am J Physiol Renal P... - June 11, 2014 Category: Urology & Nephrology Authors: Siroky BJ, Yin H, Dixon BP, Reichert RJ, Hellmann AR, Ramkumar T, Tsuchihashi Z, Bunni MA, Dillon J, Bell PD, Sampson JR, Bissler JJ Tags: Am J Physiol Renal Physiol Source Type: research

Renal blood flow and oxygenation drive nephron progenitor differentiation.
Abstract During kidney development, the vasculature develops via both angiogenesis (branching from major vessels) and vasculogenesis (de novo vessel formation). The formation and perfusion of renal blood vessels are vastly understudied. Here, we investigate the regulatory role of renal blood flow and oxygen concentration on nephron progenitor differentiation during ontogeny. To elucidate the presence of blood flow, ultrasound-guided intracardiac microinjection was performed and FITC-tagged tomato lectin was perfused through the embryo. Kidneys were co-stained for vasculature, ureteric epithelium, nephron progenito...
Source: Am J Physiol Renal P... - June 11, 2014 Category: Urology & Nephrology Authors: Rymer C, Paredes J, Halt K, Schaefer C, Wiersch J, Zhang G, Potoka DA, Vainio S, Gittes GK, Bates CM, Sims-Lucas S Tags: Am J Physiol Renal Physiol Source Type: research

Am J Physiol Renal Physiol; +16 new citations
16 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Physiol Renal Physiol These pubmed results were generated on 2014/05/30PubMed, a service of the National Library of Medicine, includes over 15 million citations for biomedical articles back to the 1950's. These citations are from MEDLINE and additional life science journals. PubMed includes links to many sites providing full text articles and other related resources. (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - May 31, 2014 Category: Urology & Nephrology Tags: Report Source Type: research

Effects of NKCC2 isoform regulation and NaCl transport in the thick ascending limb and macula densa cells: a modeling study.
This study aims to understand the extent to which modulation of NKCC2 differential splicing affects NaCl delivery to the macula densa. NaCl absorption by the thick ascending limb and macula densa cells is mediated by the apical Na+-K+-2Cl- cotransporter NKCC2. A recent study has indicated that differential splicing by NKCC2 is modulated by dietary salt (Schiebl et al., Am J Physiol Renal Physiol, 2013). Given the markedly different ion affinities of its splice variants, modulation of NKCC2 differential splicing is believed to impact NaCl reabsorption. To assess the validity of that hypothesis, we have developed a mathemati...
Source: Am J Physiol Renal P... - May 21, 2014 Category: Urology & Nephrology Authors: Edwards A, Castrop H, Laghmani K, Vallon V, Layton AT Tags: Am J Physiol Renal Physiol Source Type: research

Am J Physiol Renal Physiol; +18 new citations
18 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Physiol Renal Physiol These pubmed results were generated on 2014/05/20PubMed, a service of the National Library of Medicine, includes over 15 million citations for biomedical articles back to the 1950's. These citations are from MEDLINE and additional life science journals. PubMed includes links to many sites providing full text articles and other related resources. (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - May 20, 2014 Category: Urology & Nephrology Tags: Report Source Type: research

New Insights into the Determinants of the Serum Sodium and Risk for Dysnatremias.
Abstract editorial focus F-585-2013. PMID: 24808532 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - May 7, 2014 Category: Urology & Nephrology Authors: Rosner MH Tags: Am J Physiol Renal Physiol Source Type: research

Combination of bladder ultrasonography and novel cystometry method in mice reveals rapid decrease of bladder capacity and compliance in LPS-induced cystitis.
Abstract Various animal models have been used in research into bladder dysfunction, and in vivo cystometry is a common method to analyze bladder function in animals. However, it is rather difficult to perform reliably in small animals. Transabdominal bladder ultrasonography combined with cystometry in urethane-anesthetized mice have revealed physical inhibition of bladder wall movement by a bladder catheter conventionally placed in the bladder apex. For reliable evaluation of mouse lower urinary tract function, we established a novel cystometry method in which a catheter was placed in the bladder anterior wall, in...
Source: Am J Physiol Renal P... - May 7, 2014 Category: Urology & Nephrology Authors: Takezawa K, Kondo M, Kiuchi H, Soda T, Takao T, Miyagawa Y, Tsujimura A, Nonomura N, Shimada S Tags: Am J Physiol Renal Physiol Source Type: research

First D1-like receptor PET imaging of the rat and primate kidney: implications for human disease monitoring.
Abstract The intrarenal dopamine system is important for signaling and natriuresis, and significant dysfunction is associated with hypertension and kidney disease in ex vivo studies. Dopamine receptors also modulate and are modulated by the renin-angiotensin-aldosterone system (RAAS). Here we show the first in vivo measurement of D1-like receptors in the renal cortex of Sprague Dawley rat and Papio anubis baboon using [(11)C]NNC 112, a positron emission tomography (PET) radioligand for D1-like receptors. In addition we show a D1-like binding potential response to angiotensin II blockade in rat using losartan. Demo...
Source: Am J Physiol Renal P... - May 7, 2014 Category: Urology & Nephrology Authors: Granda ML, Schroeder FA, Borra RH, Schauer N, Aisaborhale E, Guimaraes AR, Hooker JM Tags: Am J Physiol Renal Physiol Source Type: research

pH-responsive, gluconeogenic renal epithelial LLC-PK1-FBPase+cells, a versatile in vitro model to study renal proximal tubule metabolism and function.
Abstract Ammoniagenesis and gluconeogenesis are prominent metabolic features of the renal proximal convoluted tubule that contribute to maintenance of systemic acid-base homeostasis. Molecular analysis of the mechanisms that mediate the coordinate regulation of the two pathways required development of a cell line that recapitulates these features in vitro. By adapting porcine renal epithelial LLC-PK1 cells to essentially glucose-free medium, a gluconeogenic subline, termed LLC-PK1-FBPase(+) cells, was isolated. LLC-PK1-FBPase(+) cells grow in the absence of hexoses and pentoses and exhibit enhanced oxidative metab...
Source: Am J Physiol Renal P... - May 7, 2014 Category: Urology & Nephrology Authors: Curthoys NP, Gstraunthaler G Tags: Am J Physiol Renal Physiol Source Type: research

Class I HDAC activity is required for renal protection and regeneration after acute kidney injury.
In this study, we demonstrated that all class I HDAC isoforms (1, 2, 3, and 8) were expressed in the renal epithelial cells of the mouse kidney. Inhibition of class I HDACs with MS-275, a highly selective inhibitor, resulted in more severe tubular injury in the mouse model of AKI induced by folic acid or rhabdomyolysis, as indicated by worsening renal dysfunction, increased NGAL expression, and enhanced apoptosis and caspase-3 activation. Blocking class I HDAC activity also impaired renal regeneration as evidenced by decreased expression of renal Pax-2, vimentin and proliferating cell nuclear antigen. Injury to the kidney ...
Source: Am J Physiol Renal P... - May 7, 2014 Category: Urology & Nephrology Authors: Tang J, Yan Y, Zhao TC, Gong R, Bayliss G, Yan H, Zhuang S Tags: Am J Physiol Renal Physiol Source Type: research

Dysfunction of the PGC-1α/mitochondria axis confers adriamycin-induced podocytes injury.
Dysfunction of the PGC-1α/mitochondria axis confers adriamycin-induced podocytes injury. Am J Physiol Renal Physiol. 2014 May 7; Authors: Zhu C, Xuan X, Che R, Ding G, Zhao M, Bai M, Jia Z, Huang S, Zhang A Abstract Adriamycin (ADR)-induced nephropathy in animals is an experimental analogue of human focal segmental glomerulosclerosis (FSGS), which presents as severe podocyte injury and massive proteinuria and has a poorly understood mechanism. The current study was designed to test the hypothesis that the peroxisome proliferator-activated receptor-γ coactivator 1α (PGC-1α)/mito...
Source: Am J Physiol Renal P... - May 7, 2014 Category: Urology & Nephrology Authors: Zhu C, Xuan X, Che R, Ding G, Zhao M, Bai M, Jia Z, Huang S, Zhang A Tags: Am J Physiol Renal Physiol Source Type: research

Novel Mechanisms of Sodium Retention in Obesity: Phosphorylation of NKCC2 and Regulation of SPAK/OSR1 by AMPK.
In conclusion, this study identifies increased phosphorylation of NKCC2 on S126 as a hitherto unrecognized mediator of enhanced sodium reabsorption in obesity, and identifies a new role for AMPK in regulating the activity of SPAK/OSR1. PMID: 24808538 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - May 7, 2014 Category: Urology & Nephrology Authors: Davies MR, Fraser SA, Galic S, Choy SW, Katerelos M, Gleich K, Kemp BE, Mount PF, Power DA Tags: Am J Physiol Renal Physiol Source Type: research

Role of Medullary Progenitor Cells in Epithelial Cell Migration and Proliferation.
This study is aimed at characterizing medullary interstitial progenitor cells and to examine their capacity to induce tubular epithelial cell migration and proliferation. We have isolated a progenitor cell side population from a primary medullary interstitial cell line. We show that the medullary progenitor cells (MPCs) express CD24, CD44, CXCR7, CXCR4, nestin, and PAX7. MPCs are CD34 negative, which indicates that they are not bone marrow-derived stem cells. MPCs survive more than 50 passages, and when grown in epithelial differentiation medium develop phenotypic characteristics of epithelial cells. Inner medulla collecti...
Source: Am J Physiol Renal P... - May 7, 2014 Category: Urology & Nephrology Authors: Chen D, Chen Z, Zhang Y, Park C, Al-Omari A, Moeckel GW Tags: Am J Physiol Renal Physiol Source Type: research

Role of 2',3'-Cyclic Nucleotide 3'-Phosphodiesterase in the Renal 2',3'-cAMP-Adenosine Pathway.
Conclusion: Kidneys express CNPase, and renal CNPase mediates in part the renal 2',3'-cAMP-adenosine pathway. Key Words: 2',3'-cyclic adenosine monophosphate; 2'-adenosine monophosphate; 3'-adenosine monophosphate; adenosine; 2',3'-cyclic nucleotide 3'-phosphodiesterase; CNPase. PMID: 24808540 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - May 7, 2014 Category: Urology & Nephrology Authors: Jackson EK, Gillespie DG, Mi Z, Cheng D, Bansal R, Janesko-Feldman K, Kochanek PM Tags: Am J Physiol Renal Physiol Source Type: research

Phosphate overload directly induces systemic inflammation and malnutrition as well as vascular calcification in uremia.
Abstract Hyperphosphatemia contributes to increased cardiovascular mortality through vascular calcification (VC) in patients with chronic kidney disease (CKD). Malnutrition and inflammation are also closely linked to increased risk of cardiovascular death in CKD. However, the effects of Pi overload on inflammation and malnutrition remain to be elucidated. The aim of the present study was to investigate the effects of dietary phosphate loading on the interaction among inflammation, malnutrition and VC in CKD. We used control rats fed normal diets and adenine-induced CKD rats fed diets with different phosphate conce...
Source: Am J Physiol Renal P... - May 7, 2014 Category: Urology & Nephrology Authors: Yamada S, Tokumoto M, Tatsumoto N, Taniguchi M, Noguchi H, Nakano T, Masutani K, Ooboshi H, Tsuruya K, Kitazono T Tags: Am J Physiol Renal Physiol Source Type: research

Letter to the editor: "Quantifying albumin permeability with multiphoton microscopy: why the difference?"
Letter to the editor: "Quantifying albumin permeability with multiphoton microscopy: why the difference?" Am J Physiol Renal Physiol. 2014 May;306(9):F1098-F1100 Authors: Sandoval RM, Molitoris BA PMID: 24785957 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - May 1, 2014 Category: Urology & Nephrology Authors: Sandoval RM, Molitoris BA Tags: Am J Physiol Renal Physiol Source Type: research

Reply to "Letter to the editor: 'Quantifying albumin permeability with multiphoton microscopy: why the difference?'"
Reply to "Letter to the editor: 'Quantifying albumin permeability with multiphoton microscopy: why the difference?'" Am J Physiol Renal Physiol. 2014 May;306(9):F1101-F1103 Authors: Castrop H PMID: 24785958 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - May 1, 2014 Category: Urology & Nephrology Authors: Castrop H Tags: Am J Physiol Renal Physiol Source Type: research

Reply to "Letter to the editor: 'Quantifying albumin permeability with multiphoton microscopy: why the difference?'"
Reply to "Letter to the editor: 'Quantifying albumin permeability with multiphoton microscopy: why the difference?'" Am J Physiol Renal Physiol. 2014 May;306(9):F1104-F1105 Authors: Piston DW PMID: 24785959 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - May 1, 2014 Category: Urology & Nephrology Authors: Piston DW Tags: Am J Physiol Renal Physiol Source Type: research

Sodium and Potassium Regulate Endothelial Phospholipase C Gamma and Bmx.
Abstract The amount of sodium and potassium in the diet promotes significant changes in endothelial cell function. In the present study, a series of in vitro and in vivo experiments determined the role of sodium and potassium in the regulation of two Pleckstrin Homology domain-containing intracellular signaling molecules - phospholipase C gamma-1 (PLCγ-1) and Epithelial and endothelial tyrosine kinase/Bone marrow tyrosine kinase on chromosome X (Bmx) - and agonist-generated calcium signaling in the endothelium. Extracellular [K(+)] regulated the levels of activated PLCγ-1, Bmx and carbachol-stimulated ...
Source: Am J Physiol Renal P... - April 30, 2014 Category: Urology & Nephrology Authors: Ying WZ, Aaron KJ, Sanders PW Tags: Am J Physiol Renal Physiol Source Type: research

Syncytial communication in descending vasa recta includes myoendothelial coupling.
Abstract Using dual cell patch clamp recording, we examined pericyte, endothelial and myoendothelial cell-to-cell communication in descending vasa recta. Graded current injections into pericytes or endothelia yielded input resistances of 220 ± 21 and 128 ± 20 MOhm, respectively (P
Source: Am J Physiol Renal P... - April 30, 2014 Category: Urology & Nephrology Authors: Zhang Z, Payne K, Pallone TL Tags: Am J Physiol Renal Physiol Source Type: research

LIM homeobox transcription factor 1B expression affects renal interstitial fibrosis and apoptosis in unilateral ureteral obstructed rats.
In conclusion, reduced LMX1B levels clearly represent a risk factor for renal fibrosis, whereas overexpression affords some level of protection. In general, LMX1B may be considered to be a negative regulator of the fibrosis index, transforming growth factor-βl, collagen-III, fibronectin, cleaved caspase-3, cell apoptosis, reactive oxygen species and malon dialdehyde (r= -0.756, -0.698, -0.921, -0.923, -0.843, -0.794, -0.883, -0.825; each P
Source: Am J Physiol Renal P... - April 30, 2014 Category: Urology & Nephrology Authors: Zhou TB, Ou C, Qin YH, Lei FY, Huang WF, Drummen GP Tags: Am J Physiol Renal Physiol Source Type: research

Am J Physiol Renal Physiol; +19 new citations
19 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Physiol Renal Physiol These pubmed results were generated on 2014/04/29PubMed, a service of the National Library of Medicine, includes over 15 million citations for biomedical articles back to the 1950's. These citations are from MEDLINE and additional life science journals. PubMed includes links to many sites providing full text articles and other related resources. (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - April 29, 2014 Category: Urology & Nephrology Tags: Report Source Type: research