Phosphate overload directly induces systemic inflammation and malnutrition as well as vascular calcification in uremia.
Abstract Hyperphosphatemia contributes to increased cardiovascular mortality through vascular calcification (VC) in patients with chronic kidney disease (CKD). Malnutrition and inflammation are also closely linked to increased risk of cardiovascular death in CKD. However, the effects of Pi overload on inflammation and malnutrition remain to be elucidated. The aim of the present study was to investigate the effects of dietary phosphate loading on the interaction among inflammation, malnutrition and VC in CKD. We used control rats fed normal diets and adenine-induced CKD rats fed diets with different phosphate conce...
Source: Am J Physiol Renal P... - May 7, 2014 Category: Urology & Nephrology Authors: Yamada S, Tokumoto M, Tatsumoto N, Taniguchi M, Noguchi H, Nakano T, Masutani K, Ooboshi H, Tsuruya K, Kitazono T Tags: Am J Physiol Renal Physiol Source Type: research

Letter to the editor: "Quantifying albumin permeability with multiphoton microscopy: why the difference?"
Letter to the editor: "Quantifying albumin permeability with multiphoton microscopy: why the difference?" Am J Physiol Renal Physiol. 2014 May;306(9):F1098-F1100 Authors: Sandoval RM, Molitoris BA PMID: 24785957 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - May 1, 2014 Category: Urology & Nephrology Authors: Sandoval RM, Molitoris BA Tags: Am J Physiol Renal Physiol Source Type: research

Reply to "Letter to the editor: 'Quantifying albumin permeability with multiphoton microscopy: why the difference?'"
Reply to "Letter to the editor: 'Quantifying albumin permeability with multiphoton microscopy: why the difference?'" Am J Physiol Renal Physiol. 2014 May;306(9):F1101-F1103 Authors: Castrop H PMID: 24785958 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - May 1, 2014 Category: Urology & Nephrology Authors: Castrop H Tags: Am J Physiol Renal Physiol Source Type: research

Reply to "Letter to the editor: 'Quantifying albumin permeability with multiphoton microscopy: why the difference?'"
Reply to "Letter to the editor: 'Quantifying albumin permeability with multiphoton microscopy: why the difference?'" Am J Physiol Renal Physiol. 2014 May;306(9):F1104-F1105 Authors: Piston DW PMID: 24785959 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - May 1, 2014 Category: Urology & Nephrology Authors: Piston DW Tags: Am J Physiol Renal Physiol Source Type: research

Sodium and Potassium Regulate Endothelial Phospholipase C Gamma and Bmx.
Abstract The amount of sodium and potassium in the diet promotes significant changes in endothelial cell function. In the present study, a series of in vitro and in vivo experiments determined the role of sodium and potassium in the regulation of two Pleckstrin Homology domain-containing intracellular signaling molecules - phospholipase C gamma-1 (PLCγ-1) and Epithelial and endothelial tyrosine kinase/Bone marrow tyrosine kinase on chromosome X (Bmx) - and agonist-generated calcium signaling in the endothelium. Extracellular [K(+)] regulated the levels of activated PLCγ-1, Bmx and carbachol-stimulated ...
Source: Am J Physiol Renal P... - April 30, 2014 Category: Urology & Nephrology Authors: Ying WZ, Aaron KJ, Sanders PW Tags: Am J Physiol Renal Physiol Source Type: research

Syncytial communication in descending vasa recta includes myoendothelial coupling.
Abstract Using dual cell patch clamp recording, we examined pericyte, endothelial and myoendothelial cell-to-cell communication in descending vasa recta. Graded current injections into pericytes or endothelia yielded input resistances of 220 ± 21 and 128 ± 20 MOhm, respectively (P
Source: Am J Physiol Renal P... - April 30, 2014 Category: Urology & Nephrology Authors: Zhang Z, Payne K, Pallone TL Tags: Am J Physiol Renal Physiol Source Type: research

LIM homeobox transcription factor 1B expression affects renal interstitial fibrosis and apoptosis in unilateral ureteral obstructed rats.
In conclusion, reduced LMX1B levels clearly represent a risk factor for renal fibrosis, whereas overexpression affords some level of protection. In general, LMX1B may be considered to be a negative regulator of the fibrosis index, transforming growth factor-βl, collagen-III, fibronectin, cleaved caspase-3, cell apoptosis, reactive oxygen species and malon dialdehyde (r= -0.756, -0.698, -0.921, -0.923, -0.843, -0.794, -0.883, -0.825; each P
Source: Am J Physiol Renal P... - April 30, 2014 Category: Urology & Nephrology Authors: Zhou TB, Ou C, Qin YH, Lei FY, Huang WF, Drummen GP Tags: Am J Physiol Renal Physiol Source Type: research

Am J Physiol Renal Physiol; +19 new citations
19 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Physiol Renal Physiol These pubmed results were generated on 2014/04/29PubMed, a service of the National Library of Medicine, includes over 15 million citations for biomedical articles back to the 1950's. These citations are from MEDLINE and additional life science journals. PubMed includes links to many sites providing full text articles and other related resources. (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - April 29, 2014 Category: Urology & Nephrology Tags: Report Source Type: research

The recruitment and subsequent proliferation of bone marrow-derived cells in postischemic kidney are important to the progression of fibrosis.
Abstract Acute kidney injury (AKI) is an independent risk factor of the development of chronic kidney disease (CKD). Kidney fibrosis is a typical feature of CKD and is characterized as an expansion of interstitium due to increases in extracellular matrix molecules and interstitial cells caused by accumulations of extrarenal cells and by the proliferation or differentiation of intrarenal cells. However, the role of bone marrow-derived cells (BMDCs) in AKI-induced kidney fibrosis remains to be defined. Here, we investigated the role of BMDCs in kidney fibrosis following ischemia/reperfusion injury (IRI)-induced AKI ...
Source: Am J Physiol Renal P... - April 16, 2014 Category: Urology & Nephrology Authors: Jang HS, Kim JI, Han SJ, Park KM Tags: Am J Physiol Renal Physiol Source Type: research

Active removal of inorganic phosphate from cerebrospinal fluid by the choroid plexus.
The objective of the present study was to characterize the possible role of the choroid plexus (CP) in determining CSF [Pi]. The large sheet-like IVth CP of the shark was mounted in Ussing chambers where unidirectional (33)Pi fluxes revealed potent active transport from CSF to blood side under short-circuited conditions. The flux ratio was 8:1 with an average transepithelial resistance of 87 ± 17.9 Ω x cm(2) and electrical potential difference of +0.9 ± 0.17 mV, CSF side positive. The active Pi absorption from CSF was inhibited by 10 mM arsenate, 0.2 mM ouabain, Na(+)-free medium, and by increasing [K(+...
Source: Am J Physiol Renal P... - April 16, 2014 Category: Urology & Nephrology Authors: Guerreiro PM, Bataille AM, Parker SL, Renfro JL Tags: Am J Physiol Renal Physiol Source Type: research

COX-2 Mediates Angiotensin II-Induced (Pro)Renin Receptor Expression in the Rat Renal Medulla.
Abstract (Pro)renin receptor (PRR) is predominantly expressed in the distal nephron where it is activated by angiotensin II (AngII), resulting in increased renin activity in the renal medulla thereby amplifying the de novo generation and action of local AngII. The goal of the present study was to test the role of cycloxygenase-2 (COX-2) in meditating AngII-induced PRR expression in the renal medulla in vitro and in vivo. Exposure of primary rat inner medullary collecting duct (IMCD) cells to AngII induced sequential increases in COX-2 and PRR protein expression. When the cells were pretreated with a COX-2 inhibito...
Source: Am J Physiol Renal P... - April 16, 2014 Category: Urology & Nephrology Authors: Wang F, Lu X, Peng K, Zhou L, Li C, Wang W, Yu X, Kohan DE, Zhou SF, Yang T Tags: Am J Physiol Renal Physiol Source Type: research

Hemodialysis restored iron distribution which was sequestered in spleen by bilateral nephrectomy.
Conclusions: BNx moved iron from hemoglobin and the plasma to the spleen, which is associated with an increase in plasma hepcidin. A single HD session accelerated the release of iron from the spleen, and the increased plasma iron was linked to the removal of hepcidin. Our data suggested that hepcidin might dynamically modulate the iron metabolism in BNx as well as in HD. PMID: 24740789 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - April 16, 2014 Category: Urology & Nephrology Authors: Kida A, Kuragano T, Furuta M, Otaki Y, Hasuike Y, Matsuda S, Akaike N, Kokuba Y, Nakanishi T Tags: Am J Physiol Renal Physiol Source Type: research

Phosphodiesterase V Inhibition Ameliorates Angiontensin II-induced Podocyte Dysmotility via the Protein Kinase G-mediated Downregulation of TRPC6 Activity.
Abstract The emerging role of TRPC6 as a central contributor to various pathologic processes affecting podocytes has generated interest in the development of therapeutics to modulate its function. Recent insights into the regulation of TRPC6 have revealed protein kinase G (PKG) as a potent negative modulator of TRPC6 conductance and associated signaling via its phosphorylation at 2 highly conserved amino acid residues, threonine 69 (Thr69 in murines; Thr70 in humans) and serine 321 (Ser321 in murines; Ser322 in humans). Here, we tested the role of PKG in modulating TRPC6-dependent responses in primary and conditio...
Source: Am J Physiol Renal P... - April 16, 2014 Category: Urology & Nephrology Authors: Hall G, Rowell J, Farinelli F, Gbadegesin RA, Lavin P, Wu G, Homstad A, Malone AF, Lindsey T, Jiang R, Spurney R, Tomaselli GF, Kass DA, Winn MP Tags: Am J Physiol Renal Physiol Source Type: research

Mechanisms of AT1a receptor-mediated uptake of angiotensin II by proximal tubule cells: a novel role of the multi-ligand endocytic receptor megalin.
Abstract The present study tested the hypothesis that the multi-ligand endocytic receptor megalin is partially involved in the uptake of angiotensin II (ANG II) and downstream signaling responses in mouse proximal tubule cells (mPCT) by interacting with AT1a receptors. mPCT cells of wild-type (WT) and AT1a receptor-deficient (AT1a-KO) mice were treated with vehicle, the AT1 receptor blocker losartan (10 µM), or a selective megalin siRNA for 48 h. The uptake of fluorescein (FITC)-labeled ANG II (10 nM, 37(o)C) and downstream signaling responses were analyzed by fluorescence imaging and western blot. AT1a rece...
Source: Am J Physiol Renal P... - April 16, 2014 Category: Urology & Nephrology Authors: Li XC, Zhuo JL Tags: Am J Physiol Renal Physiol Source Type: research

Mesangial Cell αvβ8 Integrin Regulates Glomerular Capillary Integrity and Repair.
Mesangial Cell αvβ8 Integrin Regulates Glomerular Capillary Integrity and Repair. Am J Physiol Renal Physiol. 2014 Apr 16; Authors: Lakhe-Reddy S, Li V, Arnold TD, Khan S, Schelling JR Abstract Integrin αvβ8 is most abundantly expressed in kidney, brain and female reproductive organs, and its cognate ligand is latent transforming growth factor-β (LTGFβ). Kidney αvβ8 localizes to mesangial cells, and global Itgb8 deletion results in embryonic lethality due to impaired placentation and cerebral hemorrhage. To circumvent the lethality, and better define kidney &...
Source: Am J Physiol Renal P... - April 16, 2014 Category: Urology & Nephrology Authors: Lakhe-Reddy S, Li V, Arnold TD, Khan S, Schelling JR Tags: Am J Physiol Renal Physiol Source Type: research

Natriuretic peptides buffer renin-dependent hypertension.
Abstract The renin-angiotensin-aldosterone system and cardiac natriuretic peptides (atrial and B-type natriuretic peptide, ANP / BNP) are opposing control mechanisms for arterial blood pressure. Accordingly, an inverse relationship between the plasma concentrations of renin (PRC) and ANP exists in most circumstances. However, PRC and ANP levels are both elevated in renovascular hypertension. Because ANP can directly suppress renin release, we used ANP knockout mice (ANP(-/-)) to investigate whether high ANP levels attenuate the increase in PRC in response to renal hypoperfusion, thus buffering renovascular hyperte...
Source: Am J Physiol Renal P... - April 9, 2014 Category: Urology & Nephrology Authors: Demerath T, Staffel J, Schreiber A, Valletta D, Schweda F Tags: Am J Physiol Renal Physiol Source Type: research

Individuality of the plasma sodium concentration.
Abstract Older literature suggested that the plasma sodium concentration is not individual - that it is neither intrinsic to an individual nor reproducible, longitudinally. We recently observed that plasma sodium concentration is heritable. Because demonstrable heritability requires individuality of the relevant phenotype, we hypothesized that plasma sodium concentration was substantially individual. In two large health plan-based cohorts, we demonstrated individuality of the plasma sodium concentration over a ten-year interval; intraclass correlation coefficient (ICC) averaged 0.4 - 0.5. Individuality of plasma s...
Source: Am J Physiol Renal P... - April 9, 2014 Category: Urology & Nephrology Authors: Zhang Z, Duckart J, Slatore CG, Fu Y, Petrik AF, Thorp ML, Cohen DM Tags: Am J Physiol Renal Physiol Source Type: research

Spontaneous Voiding by Mice Reveals Strain-Specific Lower Urinary Tract Function to be a Quantitative Genetic Trait.
Abstract Lower urinary tract symptoms (LUTS) become prevalent with aging and affect millions; however, therapy is often ineffective because etiology is unknown. Existing assays of LUT function in animal models are often invasive, however a non-invasive assay is required to study symptom progression and determine genetic correlates. Here we present a spontaneous voiding assay which is simple, reproducible, quantitative and non-invasive. Young females from eight strains of inbred mice - 129S1/SvImJ, A/J, C57BL/6J, NOD/ShiLtJ, NZO/H1LtJ, CAST/EiJ, PWK/PhJ, and WSB/EiJ - were tested for urination patterns on filter pa...
Source: Am J Physiol Renal P... - April 9, 2014 Category: Urology & Nephrology Authors: Yu W, Ackert-Bicknell C, Larigakis JD, Maciver B, Steers WD, Churchill GA, Hill WG, Zeidel ML Tags: Am J Physiol Renal Physiol Source Type: research

Albumin inhibits the insulin-mediated ACE2 increase in cultured podocytes.
er MJ Abstract Podocytes are key cells in the glomerular filtration barrier with a major role in the development of diabetic nephropathy. Podocytes are insulin-sensitive cells and have a functionally active local renin-angiotensin system. The presence and activity of angiotensin-converting enzyme 2 (ACE2), the main role of which is cleaving pro-fibrotic and pro-inflammatory angiotensin-II into angiotensin-(1-7), has been demonstrated in podocytes. Conditionally immortalized mouse podocytes were cultured with insulin in the presence and absence of albumin. We found that insulin increases ACE2 gene and protein expre...
Source: Am J Physiol Renal P... - March 26, 2014 Category: Urology & Nephrology Authors: Márquez E, Riera M, Pascual J, Soler MJ Tags: Am J Physiol Renal Physiol Source Type: research

OMA1 mediates OPA1 proteolysis and mitochondrial fragmentation in experimental models of ischemic kidney injury.
In this study, we have determined the role of OMA1 in OPA1 proteolysis and mitochondrial fragmentation in experimental models of ischemic AKI. In ATP-depletion injury, knockdown of OMA1 suppressed OPA1 proteolysis, mitochondrial fragmentation, cytochrome c release and consequent apoptosis in renal proximal tubular cells. In mice, OMA1-deficiency prevented ischemic AKI as indicated by better renal function, less tubular damage, and lower apoptosis. OPA1 proteolysis and mitochondrial injury during ischemic AKI were ameliorated in OMA1-deficient mice. Thus OMA1-mediated OPA1 proteolysis plays an important role in the disrupti...
Source: Am J Physiol Renal P... - March 26, 2014 Category: Urology & Nephrology Authors: Xiao X, Hu Y, Quirós PM, Wei Q, Lopez-Otin C, Dong Z Tags: Am J Physiol Renal Physiol Source Type: research

Am J Physiol Renal Physiol; +19 new citations
19 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Physiol Renal Physiol These pubmed results were generated on 2014/03/20PubMed, a service of the National Library of Medicine, includes over 15 million citations for biomedical articles back to the 1950's. These citations are from MEDLINE and additional life science journals. PubMed includes links to many sites providing full text articles and other related resources. (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - March 20, 2014 Category: Urology & Nephrology Tags: Report Source Type: research

Na+-K+-2Cl- co-transporter-mediated fluid secretion increases under hypotonic osmolarity in the mouse submandibular salivary gland.
In this study, the effects of a hypo-osmotic challenge on carbachol (CCh)-induced fluid secretion was investigated using an ex vivo submandibular gland perfusion technique and intracellular pH and Ca(2+) measurements. The osmolality of the perfusion solution was altered to examine the response of the gland to a hypotonic challenge. The flow rate was increased by 34% with a 30% hypotonic solution (225 mOsm), although the Ca(2+) response was unchanged. Lowering the external Cl(-) by 50% abolished this increase in the 30% hypotonic solution. Furthermore, bumetanide, an inhibitor of the Na(+)-K(+)-2Cl(-) co-transporter, comple...
Source: Am J Physiol Renal P... - March 12, 2014 Category: Urology & Nephrology Authors: Kidokoro M, Nakamoto T, Mukaibo T, Kondo Y, Munemasa T, Imamura A, Masaki C, Hosokawa R Tags: Am J Physiol Renal Physiol Source Type: research

High glucose and diabetes enhanced store-operated ca2+ entry and increased expression of its siganling proteins in mesangial cells.
HIGH GLUCOSE AND DIABETES ENHANCED STORE-OPERATED CA2+ ENTRY AND INCREASED EXPRESSION OF ITS SIGANLING PROTEINS IN MESANGIAL CELLS. Am J Physiol Renal Physiol. 2014 Mar 12; Authors: Chaudhari S, Wu P, Wang Y, Ding Y, Yuan J, Begg M, Ma R Abstract The present study was conducted to determine whether and how store-operated Ca(2+) entry (SOCE) in glomerular mesangial cells (MCs) was altered by high glucose (HG) and diabetes. Human MCs were treated with either normal glucose or HG for different time periods. Cyclopiazonic acid-induced SOCE was significantly greater in the MCs with 7 day HG treatment and the re...
Source: Am J Physiol Renal P... - March 12, 2014 Category: Urology & Nephrology Authors: Chaudhari S, Wu P, Wang Y, Ding Y, Yuan J, Begg M, Ma R Tags: Am J Physiol Renal Physiol Source Type: research

Human mesenchymal stem cells alter macrophage phenotype and promote regeneration via homing to the kidney following ischemia/reperfusion injury.
In conclusion, MSCs home to injured kidneys and promote repair, which may be mediated by their ability to promote M2 macrophage polarization. PMID: 24623144 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - March 12, 2014 Category: Urology & Nephrology Authors: Wise AF, Williams TM, Kiewiet MB, Payne NL, Siatskas C, Samuel CS, Ricardo SD Tags: Am J Physiol Renal Physiol Source Type: research

Endogenous Tim-1 promotes severe systemic autoimmunity and renal disease MRL-Fas{uparrow}lpr mice.
This study investigated the effects of an inhibitory anti-T-cell immunoglobulin mucin 1 antibody (RMT1-10) in lupus-prone MRL-Fas(lpr) mice. MRL-Fas(lpr) mice were treated with RMT1-10 or a control antibody intraperitoneally twice weekly from three mo of age for sixteen wks. RMT1-10 treatment significantly improved survival, limited the development of lymphadenopathy and skin lesions, preserved renal function and decreased proteinuria, reduced serum anti-DNA antibody levels and attenuated renal leukocyte accumulation. Th1 and Th17 cellular responses systemically and intrarenally were reduced, but regulatory T and B cells w...
Source: Am J Physiol Renal P... - March 12, 2014 Category: Urology & Nephrology Authors: Nozaki Y, Kitching AR, Akiba H, Yagita H, Kinoshita K, Funauchi M, Matsumura I Tags: Am J Physiol Renal Physiol Source Type: research

Silencing of Hypoxia Inducible Factor-1α Gene Attenuates Chronic Ischemic Renal Injury in Two-Kidney One-Clip Rats.
Conclusion: long-term over-activation of HIF-1α is a pathogenic factor in chronic renal injury associated with ischemia/hypoxia. PMID: 24623146 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - March 12, 2014 Category: Urology & Nephrology Authors: Wang Z, Zhu Q, Li PL, Dhaduk R, Zhang F, Gehr TW, Li N Tags: Am J Physiol Renal Physiol Source Type: research

Differences in susceptibility to develop parameters of diabetic nephropathy in four mouse strains with type 1 diabetes.
In conclusion, diabetic C57Bl/6 and NMRI both developed glomerular hyperfiltration, but neither presented with histological damage, although NMRI developed low-degree proteinuria. Thus, these strains may be suitable when investigating the mechanism causing hyperfiltration. Neither BALB/c nor 129Sv developed hyperfiltration although both developed pronounced proteinuria. However, only BALB/c developed detectable histological damage. Thus, BALB/c may be suitable when studying the roles of proteinuria and histological alterations for the progression of diabetic nephropathy. PMID: 24623147 [PubMed - as supplied by publish...
Source: Am J Physiol Renal P... - March 12, 2014 Category: Urology & Nephrology Authors: Franzén S, Friederich-Persson M, Fasching A, Hansell P, Nangaku M, Palm F Tags: Am J Physiol Renal Physiol Source Type: research

Renal vasoconstriction by vasopressin V1a receptors is modulated by nitric oxide, prostanoids, and superoxide but not the ADP ribosyl cyclase CD38.
Abstract Renal blood flow (RBF) responses to arginine vasopressin (AVP) were tested in anesthetized wild-type (WT) and CD38(-/-) mice that lack the major calcium mobilizing second messenger cyclic ADP ribose. AVP (3-25 ng) injected iv produced dose-dependent decreases in RBF, reaching a maximum of 25±2% below basal RBF in WT and 27±2% in CD38(-/-) mice with 25 ng of AVP. Renal vascular resistance (RVR) increased 75±6% and 78±6% in WT and CD38(-/-) mice. Inhibition of NO synthase with L-NAME increased the maximum RVR response to AVP to 308±76% in WT and 388±81% in CD38(-/-) (P
Source: Am J Physiol Renal P... - March 12, 2014 Category: Urology & Nephrology Authors: Moss NG, Kopple TE, Arendshorst WJ Tags: Am J Physiol Renal Physiol Source Type: research

Renal Section Young Investigator Award.
Abstract NA. PMID: 24623149 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - March 12, 2014 Category: Urology & Nephrology Authors: Welch WJ Tags: Am J Physiol Renal Physiol Source Type: research

Theoretical Assessment of Renal Autoregulatory Mechanisms.
Abstract A mathematical model of renal hemodynamics is used to assess the individual contributions of the tubuloglomerular feedback (TGF) mechanism and the myogenic response to glomerular filtration rate regulation in the rat kidney. The model represents an afferent arteriole segment, glomerular filtration, and a short loop of Henle. The afferent arteriole model exhibits myogenic response, which is activated by hydrostatic pressure variations to induce changes in membrane potential and vascular muscle tone. The tubule model predicts tubular fluid and Cl00; transport. Macula densa Cl00; concentration is sensed as t...
Source: Am J Physiol Renal P... - March 12, 2014 Category: Urology & Nephrology Authors: Sgouralis I, Layton AT Tags: Am J Physiol Renal Physiol Source Type: research

Am J Physiol Renal Physiol; +21 new citations
21 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Physiol Renal Physiol These pubmed results were generated on 2014/03/10PubMed, a service of the National Library of Medicine, includes over 15 million citations for biomedical articles back to the 1950's. These citations are from MEDLINE and additional life science journals. PubMed includes links to many sites providing full text articles and other related resources. (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - March 10, 2014 Category: Urology & Nephrology Tags: Report Source Type: research

Am J Physiol Renal Physiol; +16 new citations
16 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Physiol Renal Physiol These pubmed results were generated on 2014/03/05PubMed, a service of the National Library of Medicine, includes over 15 million citations for biomedical articles back to the 1950's. These citations are from MEDLINE and additional life science journals. PubMed includes links to many sites providing full text articles and other related resources. (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - March 6, 2014 Category: Urology & Nephrology Tags: Report Source Type: research

Human podocytes perform polarized, caveolae-dependent albumin endocytosis.
Abstract The renal glomerulus forms a selective filtration barrier that allows the passage of water, ions and small solutes into the urinary space while restricting the passage of cells and macromolecules. The three layers of the glomerular filtration barrier include the vascular endothelium, glomerular basement membrane (GBM) and podocyte epithelium. Podocytes are capable of internalizing albumin and are hypothesized to clear proteins that traverse the GBM. The present study followed the fate of FITC-labeled albumin to establish the mechanisms of albumin endocytosis and processing by podocytes. Confocal imaging a...
Source: Am J Physiol Renal P... - February 26, 2014 Category: Urology & Nephrology Authors: Dobrinskikh E, Okamura K, Kopp JB, Doctor RB, Blaine J Tags: Am J Physiol Renal Physiol Source Type: research

Persistence of 1,25D-Induced Hypercalciuria in Alendronate Treated Genetic Hypercalciuric Stone-Forming Rats Fed a Low Calcium Diet.
Abstract Genetic hypercalciuric stone-forming (GHS) rats demonstrate increased intestinal Ca absorption, increased bone resorption and reduced renal tubular Ca reabsorption leading to hypercalciuria, and all form kidney stones. GHS have increased vitamin D receptors (VDR) at these sites of Ca transport. Injection of 1,25(OH)2D3 (1,25D) leads to a greater increase in uCa in GHS than in control Sprague-Dawley (SD), possibly due to the additional VDR. In GHS the increased uCa persists on a low Ca diet (LCD) suggesting enhanced bone resorption. We tested the hypothesis that LCD, coupled to inhibition of bone resorptio...
Source: Am J Physiol Renal P... - February 26, 2014 Category: Urology & Nephrology Authors: Frick KK, Asplin JR, Culbertson CD, Granja I, Krieger NS, Bushinsky DA Tags: Am J Physiol Renal Physiol Source Type: research

Increasing cGMP-dependent protein kinase activity attenuates unilateral ureteral obstruction-induced renal fibrosis.
Abstract Our previous studies support the protective effect of cGMP and cGMP-dependent protein kinase I (PKG-I) pathway on the development of renal fibrosis. Therefore, in the present studies, we determined whether pharmacologically or genetically increased PKG activity attenuates renal fibrosis in a unilateral ureteral obstruction (UUO) model and also examined the mechanisms involved. To increase PKG activity, we used the phosphodiesterase 5 (PDE5) inhibitor-sildenafil and PKG transgenic mice. UUO model was induced in wild type or PKG-I transgenic mice by ligating the left lateral ureteral and the renal fibrosis ...
Source: Am J Physiol Renal P... - February 26, 2014 Category: Urology & Nephrology Authors: Cui W, Maimaitiyiming H, Qi X, Norman H, Zhou Q, Wang X, Fu J, Wang S Tags: Am J Physiol Renal Physiol Source Type: research

Activation of ERK1/2 by NADPH oxidase-originated reactive oxygen species mediates uric acid-induced mesangial cell proliferation.
In conclusion, UA-induced MC proliferation was mediated by NADPH/ROS/ERK1/2 signaling pathway. This novel finding not only reveals the mechanism of UA-induced MC cell proliferation, but also provides some potential targets for future treatment of UA-related glomerular injury. PMID: 24573389 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - February 26, 2014 Category: Urology & Nephrology Authors: Zhuang Y, Feng Q, Ding G, Zhao M, Che R, Bai M, Bao H, Zhang A, Huang S Tags: Am J Physiol Renal Physiol Source Type: research

Novel MAPK-dependent and independent tubulogenes identified via microarray analysis of 3D-cultured Madin-Darby canine kidney cells.
Abstract Cystogenesis and tubulogenesis are basic building blocks for many epithelial organs, including the kidney. Most researchers have used two-dimensional (2D) cell culture to investigate signaling pathways downstream of hepatocyte growth factor (HGF). We hypothesize that three-dimensional (3D) collagen-grown Madin-Darby canine kidney (MDCK) cells, which form cysts and then tubulate in response to hepatocyte growth factor (HGF), is a much more in vivo-like system for the identification of novel tubulogenes. Using a canine microarray containing over 20,000 genes, 2,417 genes were identified as potential tubulog...
Source: Am J Physiol Renal P... - February 26, 2014 Category: Urology & Nephrology Authors: Chacon-Heszele MF, Zuo X, Hellman NE, McKenna S, Choi SY, Huang L, Tobias JW, Park KM, Lipschutz JH Tags: Am J Physiol Renal Physiol Source Type: research

2014 Carl W. Gottschalk Distinguished Lecturer of the American Physiological Society Renal Section.
Abstract winner. PMID: 24573391 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - February 26, 2014 Category: Urology & Nephrology Authors: Sands JM Tags: Am J Physiol Renal Physiol Source Type: research

Renal tubular Notch signaling triggers a pro-senescent state after acute kidney injury.
In conclusion, our data suggest that sustained Notch activation after IR might contribute to the inferior outcome of old kidneys after injury. Sustained epithelial activation of Notch is associated with a pro-senescent phenotype and a maladaptive repair. PMID: 24573392 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - February 26, 2014 Category: Urology & Nephrology Authors: Soerensen-Zender I, Rong S, Susnik N, Zender S, Pennekamp P, Melk A, Haller H, Schmitt R Tags: Am J Physiol Renal Physiol Source Type: research

Renal-specific and inducible depletion of NaPi-IIc/Slc34A3, the cotransporter mutated in HHRH, does not affect phosphate or calcium homeostasis in mice.
Abstract The proximal renal epithelia express three different Na-dependent inorganic phosphate (Pi)-cotransporters: NaPi-IIa/SLC34A1, NaPi-IIc/SLC34A3 and PiT2/SLC20A2. Constitutive mouse knockout models of NaPi-IIa and NaPi-IIc suggested that NaPi-IIa mediates the bulk of renal reabsorption of Pi whereas the contribution of NaPi-IIc to this process is minor and probably restricted to young mice. However, many reports indicate that mutations of NaPi-IIc in humans lead to Hereditary Hypophosphatemic Rickets with Hypercalciuria (HHRH). Here we report the generation of a kidney-specific and inducible NaPi-IIc deficie...
Source: Am J Physiol Renal P... - February 19, 2014 Category: Urology & Nephrology Authors: Myakala K, Motta S, Murer H, Wagner CA, Koesters R, Biber J, Hernando N Tags: Am J Physiol Renal Physiol Source Type: research

Regulation of the Proximal Tubule Vacuolar H+-ATPase by PKA and AMP-Activated Protein Kinase.
We examined the role of PKA and AMP-activated protein kinase (AMPK) in the regulation of the V-ATPase in the proximal tubule as these two kinases co-regulate the V-ATPase in the collecting duct. As the proximal tubule V-ATPases have different subunit compositions from other nephron segments, we postulated that V-ATPase regulation in the proximal tubule could differ from other kidney tubule segments. Immunofluorescence labeling of rat ex vivo kidney slices revealed that the V-ATPase was present in the proximal tubule both at the apical pole, co-localizing with the brush border marker wheat germ agglutinin, and in the cytoso...
Source: Am J Physiol Renal P... - February 19, 2014 Category: Urology & Nephrology Authors: Al-Bataineh MM, Gong F, Marciszyn A, Myerburg MM, Pastor-Soler NM Tags: Am J Physiol Renal Physiol Source Type: research

ATP acting through P2Y receptors causes activation of podocyte TRPC6 channels: Role of podocin and reactive oxygen species.
Abstract Extracellular ATP may contribute to Ca(2+) signaling in podocytes during tubuloglomerular feedback (TGF) and possibly as a result of local tissue damage. TRPC6 channels are Ca(2+)-permeable cationic channels that have been implicated in the pathophysiology of podocyte diseases. Here we show using whole-cell recordings that ATP evokes robust activation of TRPC6 channels in mouse podocyte cell lines and in rat podocytes attached to glomerular capillaries in ex vivo glomerular explants. The ED50 for ATP is around 10 μM, is maximal at 100 μM, and currents were blocked by the P2 antagonist suramin. In te...
Source: Am J Physiol Renal P... - February 19, 2014 Category: Urology & Nephrology Authors: Roshanravan H, Dryer SE Tags: Am J Physiol Renal Physiol Source Type: research

Dominant factors that govern pressure natriuresis in diuresis and antidiuresis: a mathematical model.
Abstract We have developed a whole-kidney model of the urine concentrating mechanism and renal autoregulation. The model represents the tubuloglomerular feedback (TGF) and myogenic mechanisms, which together affect the resistance of the afferent arteriole and thus glomerular filtration rate. TGF is activated by fluctuations in macula densa [Cl(-)] and myogenic mechanism by changes in hydrostatic pressure. The model was used to investigate the relative contributions of medullary blood flow autoregulation and inhibition of transport in the proximal convoluted tubule to pressure natriuresis in both diuresis and antid...
Source: Am J Physiol Renal P... - February 19, 2014 Category: Urology & Nephrology Authors: Moss R, Layton AT Tags: Am J Physiol Renal Physiol Source Type: research

Novel cardiolipin therapeutic protects endothelial mitochondria during renal ischemia and mitigates microvascular rarefaction, inflammation and fibrosis.
NOVEL CARDIOLIPIN THERAPEUTIC PROTECTS ENDOTHELIAL MITOCHONDRIA DURING RENAL ISCHEMIA AND MITIGATES MICROVASCULAR RAREFACTION, INFLAMMATION AND FIBROSIS. Am J Physiol Renal Physiol. 2014 Feb 19; Authors: Liu S, Soong Y, Seshan SV, Szeto HH Abstract Microvascular rarefaction, or loss of microvascular density, is increasingly implicated in the progression from acute ischemic kidney injury to chronic kidney disease. Microvascular dropout results in chronic tissue hypoxia, interstitial inflammation and fibrosis. There is currently no therapeutic intervention for microvascular rarefaction. We hypothesize that c...
Source: Am J Physiol Renal P... - February 19, 2014 Category: Urology & Nephrology Authors: Liu S, Soong Y, Seshan SV, Szeto HH Tags: Am J Physiol Renal Physiol Source Type: research

Modulation of angiotensin ii-induced inflammatory cytokines by epac1-rap1a-nhe3 pathway: implications in renal tubular pathobiology.
MODULATION OF ANGIOTENSIN II-INDUCED INFLAMMATORY CYTOKINES BY EPAC1-RAP1A-NHE3 PATHWAY: IMPLICATIONS IN RENAL TUBULAR PATHOBIOLOGY. Am J Physiol Renal Physiol. 2014 Feb 19; Authors: Xie P, Joladarashi D, Dudeja PK, Sun L, Kanwar YS Abstract Besides glomerulus tubulo-interstitium is often concomitantly affected in certain diseases, e.g., diabetic nephropathy, and activation of renin-angiotensin system, to a certain extent, worsens its outcome because of perturbations in hemodynamics and possibly tubulo-glomerular feedback. Certain studies suggest that pathobiology of tubulo-interstitial is influenced by sm...
Source: Am J Physiol Renal P... - February 19, 2014 Category: Urology & Nephrology Authors: Xie P, Joladarashi D, Dudeja PK, Sun L, Kanwar YS Tags: Am J Physiol Renal Physiol Source Type: research

Angiotensin 1-7 mediates renoprotection against diabetic nephropathy by reducing oxidative stress, inflammation and lipotoxicity.
Abstract The renin-angiotensin system, especially angiotensin (Ang) II, plays a key role in the development and progression of diabetic nephropathy. Ang 1-7 has counteracting effects of Ang II and is known to exert beneficial effects on diabetic nephropathy. We studied the mechanism of Ang 1-7-induced beneficial effects on diabetic nephropathy in db/db mice. We administered Ang 1-7 (0.5 mg·kg(-1)·d(-1)) or saline to 5-month old db/db mice for 28 days via implanted micro-osmotic pumps. Ang 1-7 treatment reduced kidney weight, ameliorated mesangial expansion and the increased urinary albumin excretion,...
Source: Am J Physiol Renal P... - February 19, 2014 Category: Urology & Nephrology Authors: Mori J, Patel VB, Ramprasath T, Alrob OA, Desaulniers J, Scholey JW, Lopaschuk GD, Oudit GY Tags: Am J Physiol Renal Physiol Source Type: research

Am J Physiol Renal Physiol; +21 new citations
21 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Physiol Renal Physiol These pubmed results were generated on 2014/02/14PubMed, a service of the National Library of Medicine, includes over 15 million citations for biomedical articles back to the 1950's. These citations are from MEDLINE and additional life science journals. PubMed includes links to many sites providing full text articles and other related resources. (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - February 14, 2014 Category: Urology & Nephrology Tags: Report Source Type: research

Albumin-bound fatty acids but not albumin itself alter redox balance in tubular epithelial cells and induce a peroxide-mediated redox-sensitive apoptosis.
Abstract Albuminuria is associated with metabolic syndrome and diabetes. It correlates with the progression of chronic kidney disease, particularly with tubular atrophy. The fatty acid load on albumin significantly increases in obesity, presenting a proinflammatory environment to the proximal tubules. However, little is known about changes in the redox milieu during fatty acid overload and how redox-sensitive mechanisms mediate cell death. Here, we show that albumin with fatty acid impurities or conjugated with palmitate, but not albumin itself compromised mitochondrial and cell viability, membrane potential and r...
Source: Am J Physiol Renal P... - February 5, 2014 Category: Urology & Nephrology Authors: Ruggiero C, Elks CM, Kruger C, Cleland E, Addison K, Noland RC, Stadler K Tags: Am J Physiol Renal Physiol Source Type: research

Regulation of Renalase Expression by D5 Dopamine Receptors in Rat Renal Proximal Tubule Cells.
Abstract The dopaminergic and sympathetic systems interact to regulate blood pressure. Our previous studies showed that a regulation of α1-adrenergic receptor function by D1-like dopamine receptors in vascular smooth muscle cells. Because renalase could regulate circulating epinephrine levels and dopamine production in renal proximal tubules (RPTs), we tested the hypothesis that D1-like receptors regulate renalase expression in kidney. The effect of D1-like receptor stimulation on renalase expression and function was measured in immortalized RPT cells from Wistar-Kyoto (WKY) and spontaneously hypertensive ra...
Source: Am J Physiol Renal P... - February 5, 2014 Category: Urology & Nephrology Authors: Wang S, Lu X, Yang J, Wang H, Chen C, Han Y, Ren H, Zheng S, He D, Zhou L, Asico LD, Wang WE, Jose PA, Zeng C Tags: Am J Physiol Renal Physiol Source Type: research

Down-regulation of renal type IIa sodium-dependent phosphate co-transporter during lipopolysaccharide-induced acute inflammation.
We examined the regulation of Pi metabolism and renal Npt2a expression during lipopolysaccharide (LPS)-induced inflammation in mice. Dose-response and time-course studies with LPS showed significant increases of plasma Pi and intact parathyroid hormone (iPTH) levels and renal Pi excretion; while renal calcium excretion was significantly decreased. There was no difference in plasma 1,25-dihydroxyvitamin D levels, but the induction of plasma intact fibroblast growth factor 23 levels peaked 3 h after LPS treatment. Western blotting, immunostaining, and quantitative real-time PCR showed that LPS administration significantly de...
Source: Am J Physiol Renal P... - February 5, 2014 Category: Urology & Nephrology Authors: Ikeda S, Yamamoto H, Masuda M, Takei Y, Nakahashi O, Kozai M, Tanaka S, Nakao M, Taketani Y, Segawa H, Iwano M, Miyamoto KI, Takeda E Tags: Am J Physiol Renal Physiol Source Type: research