Krt5+ Urothelial Cells are Developmental and Tissue Repair Progenitors in the Kidney.
In this study, we traced the origins of adult Upk+ RUCs during normal development and in response to UTO. Fate mapping analysis demonstrates that adult Upk+ RUCs derive from embryonic and neonatal Krt5+ RUCs, whereas Krt5+ RUCs lose this progenitor capacity and become lineage restricted by postnatal day (P)14. However, in response to UTO, P14 labeled adult Krt5+ RUCs break their lineage restriction and robustly differentiate into Upk+ RUCs. Thus, Krt5+ RUCs drive renal urothelial formation during normal ontogeny and following UTO by differentiating into Upk+ RUCs in a temporally restricted manner. PMID: 31322419 [PubM...
Source: Am J Physiol Renal P... - July 19, 2019 Category: Urology & Nephrology Authors: Jackson AR, Hoff ML, Li B, Ching CB, McHugh KM, Becknell B Tags: Am J Physiol Renal Physiol Source Type: research

Am J Physiol Renal Physiol; +19 new citations
19 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Physiol Renal Physiol These pubmed results were generated on 2019/07/17PubMed comprises more than millions of citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites. (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - July 17, 2019 Category: Urology & Nephrology Tags: Report Source Type: research

Am J Physiol Renal Physiol; +57 new citations
57 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Physiol Renal Physiol These pubmed results were generated on 2019/07/11PubMed comprises more than millions of citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites. (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - July 12, 2019 Category: Urology & Nephrology Tags: Report Source Type: research

Profiling histone modifications in the normal mouse kidney and after unilateral ureteric obstruction.
In this study, we hypothesised that unilateral ureteric obstruction (UUO), a widely used model of tubulointerstitial injury, would be associated with a distinct pattern of histone (H) modifications (marks) in the kidney. Mass spectrometry (MS) was used to profile 63 different histone marks in normal mouse kidney and those after 10 days of UUO. A subsequent histochemical analysis further examined examples of specific marks that changed significantly after UUO, for which antisera are available. Histone marks were much more widely distributed and abundant in the normal kidney than usually appreciated. Although aggregate analy...
Source: Am J Physiol Renal P... - July 3, 2019 Category: Urology & Nephrology Authors: Hewitson TD, Holt SG, Samuel CS, Wigg B, Smith ER Tags: Am J Physiol Renal Physiol Source Type: research

Mitochondrial activity contributes to impaired renal metabolic homeostasis and renal pathology in STZ-induced diabetic mice.
Abstract Diabetic nephropathy (DN) has become the main cause of end-stage renal disease worldwide while the efficacy of current therapeutic strategies on DN remains unsatisfactory. Recent researches reported the involvement of metabolic re-arrangement in the pathological process of DN, and of all the disturbances in metabolism, mitochondria serve as key regulatory hubs. In the present study, high-resolution mass spectrometry-based none-target metabolomics was employed to uncover the metabolic characteristics of early diabetic kidney with or without the inhibition of mitochondrial activity. At first, we observed a ...
Source: Am J Physiol Renal P... - July 3, 2019 Category: Urology & Nephrology Authors: Wu M, Li S, Yu X, Chen W, Ma H, Shao C, Zhang Y, Zhang A, Huang S, Jia Z Tags: Am J Physiol Renal Physiol Source Type: research

Sex Differences in Diabetes and Kidney Disease: Mechanisms and Consequences.
Abstract Type 1 and Type 2 diabetes, along with its accompanying hyperglycemia, is associated with a multitude of comorbidities including the development of diabetic kidney disease. While the hallmarks of these metabolic disorders have been well-characterized in population and animal studies, it is becoming increasingly apparent that diabetes manifests itself differently in men and women. This review summarizes the recent diabetic literature with a focus on known sex differences in clinical and preclinical studies. It explores the physiological differences of glucose handling and the development of diabetes betwee...
Source: Am J Physiol Renal P... - June 26, 2019 Category: Urology & Nephrology Authors: Shepard BD Tags: Am J Physiol Renal Physiol Source Type: research

The contribution of collecting duct NOS1 to the concentrating mechanisms in male and female mice.
In conclusion CD NOS1 plays only a minor role in urine concentrating mechanisms. PMID: 31241990 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - June 26, 2019 Category: Urology & Nephrology Authors: Mendoza LD, Hyndman KA Tags: Am J Physiol Renal Physiol Source Type: research

In human nephrectomy specimens, the kidney level of tubular transport proteins does not correlate with their abundance in urinary extracellular vesicles.
sen P Abstract Human urinary extracellular vesicles (uEVs) contain proteins from all nephron segments. An assumption for years has been that uEVs might provide a non-invasive liquid biopsy that reflect physiological regulation of transporter protein expression in human. We hypothesized that protein abundance in human kidney tissue and uEV are directly related and tested this in paired collections of nephrectomy tissue and urine sample from 12 patients. Kidney tissue was fractioned into total kidney protein, crude membrane (plasma membrane and large intracellular vesicles) and intracellular vesicle enriched fractio...
Source: Am J Physiol Renal P... - June 26, 2019 Category: Urology & Nephrology Authors: Sabaratnam R, Geertsen L, Skjødt K, Hojlund K, Dimke H, Lund L, Svenningsen P Tags: Am J Physiol Renal Physiol Source Type: research

Spironolactone reduces oxidative stress in living donor kidney transplantation: a randomized controlled trial.
In conclusion, spironolactone reduces the acute increase in urinary oxidative stress in living-donor KT recipients. PMID: 31241992 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - June 26, 2019 Category: Urology & Nephrology Authors: Morales-Buenrostro LE, Ortega Trejo JA, Pérez-Villalva R, Marino LA, Gonzalez Bobadilla Y, Juarez H, Zamora Mejia FM, Gonzalez N, Espinoza R, Barrera-Chimal J, Bobadilla NA Tags: Am J Physiol Renal Physiol Source Type: research

Monophosphoryl lipid A induces protection against LPS in medullary thick ascending limb through induction of Tollip and negative regulation of IRAK-1.
Abstract LPS inhibits HCO3- absorption in the medullary thick ascending limb (MTAL) through a TLR4-MyD88-ERK pathway that is upregulated by sepsis. Pretreatment with the nontoxic immunomodulator monophosphoryl lipid A (MPLA) prevents inhibition by LPS through activation of a TLR4-TRIF-PI3K pathway that prevents LPS-induced ERK activation. Here, we identify molecular mechanisms that underlie the protective inhibitory interaction between the MPLA-PI3K and LPS-ERK pathways. Treatment of mouse MTALs with LPS in vitro increased phosphorylation of interleukin-1 receptor-associated kinase (IRAK)-1, a critical mediator of...
Source: Am J Physiol Renal P... - June 26, 2019 Category: Urology & Nephrology Authors: Watts BA, Tamayo EH, Sherwood ER, Good DW Tags: Am J Physiol Renal Physiol Source Type: research

Smooth muscle-specific deletion of MnSOD exacerbates diabetes-induced bladder dysfunction in mice.
Abstract Bladder dysfunction in diabetes progresses gradually over time. However, the mechanisms of the development are not clear. We test the hypothesis that oxidative stress plays a key role in the development of diabetic bladder dysfunction, using an inducible smooth muscle (SM)-specific Sod2 gene knockout (SM-Sod2 KO) mouse model. Eight-week-old male Sod2lox/lox, SM-CreERT2(ki)Cre/+ mice and wild-type mice were assigned to diabetic or control groups. 4-hydroxytamoxifen was injected into Sod2lox/lox, SM-CreERT2(ki)Cre/+ mice to activate CreERT2-mediated deletion of Sod2. Diabetes was induced by injection of str...
Source: Am J Physiol Renal P... - June 26, 2019 Category: Urology & Nephrology Authors: Elrashidy RA, Kavran M, Asker ME, Mohamed HE, Daneshgari F, Liu G Tags: Am J Physiol Renal Physiol Source Type: research

APOL1 and Kidney Cell Function.
Abstract The APOL1 gene is unique to humans and gorillas and appeared approximately 33 million years ago. Since the majority of the mammals do not carry APOL1, it seems to be dispensable for kidney function. APOL1 renal risk variants (RRVs, G1 and G2) are associated with the development as well as the progression of chronic kidney diseases (CKDs) at higher rates in the population with African ancestry. Cellular expression of two APOL1 RRVs has been demonstrated to induce cytotoxicity, including necrosis, apoptosis, and pyroptosis in several cell types including podocytes; mechanistically, these toxicities were att...
Source: Am J Physiol Renal P... - June 26, 2019 Category: Urology & Nephrology Authors: Kumar V, Singhal PC Tags: Am J Physiol Renal Physiol Source Type: research

Renal T cell Infiltration Occurs Despite Attenuation of Development of Hypertension with Hydralazine in Envigo's Female Dahl Rat Maintained on a Low Sodium Diet.
Abstract Many studies suggest renal T cell infiltration contributes to the pathogenesis of salt-sensitive hypertension. To further investigate this mechanism, we determined T cell profiles in the kidney and lymphoid tissues as a function of blood pressure in the female Envigo Dahl salt-sensitive (SS) rat maintained on low-sodium (LS)-diet. Mean arterial pressure (MAP) and heart rate (HR) were measured by telemetry in SS rats from 1 (juveniles) to 4 months (mo) old. Normotensive salt-resistant (SR) rats were included as controls. The frequencies of helper (CD4+) T cells were greater in the kidney, lymph nodes (LN) ...
Source: Am J Physiol Renal P... - June 26, 2019 Category: Urology & Nephrology Authors: Pai AV, West CA, Arlindo de Souza AM, Kadam PS, Pollner EJ, West DA, Li J, Ji H, Wu X, Zhu M, Baylis C, Sandberg K Tags: Am J Physiol Renal Physiol Source Type: research

Salt-Sensitive Increase of Macrophages in the Kidneys of Dahl SS Rats.
Abstract Studies of Dahl Salt-Sensitive (SS) rats have shown that renal CD3+ T-cells and ED-1+ macrophages are involved in the development of SS hypertension and renal damage. The present study demonstrated that the increase in renal immune cells, which accompanies renal hypertrophy and albuminuria in high salt-fed Dahl SS rats, is absent in Sprague Dawley and SSBN13 rats that are protected from the SS disease phenotype. Flow cytometric analysis demonstrated that>70% of the immune cells in the SS kidney are M1 macrophages. PCR profiling of renal myeloid cells showed a salt-induced upregulation in 9 of 84 genes ...
Source: Am J Physiol Renal P... - June 19, 2019 Category: Urology & Nephrology Authors: Fehrenbach DJ, Abais-Battad JM, Dasinger JH, Lund H, Mattson DL Tags: Am J Physiol Renal Physiol Source Type: research

Heme Oxygenase-2 Protects Against Ischemic Acute Kidney Injury: The Influence of Age and Sex.
This study examined whether HO-2 is protective in ischemic AKI. Renal ischemia was imposed on young and aged HO-2+/+ and HO-2-/- mice. On days 1 and 2 after renal ischemia, there were no significant differences in renal function either between young male HO-2+/+ and HO-2-/- mice, between young female HO-2+/+ and HO-2-/- mice, or between aged female HO-2+/+ and HO-2-/- mice. However, in aged male mice, HO-2 deficiency worsened renal function on days 1 and 2 after ischemic AKI, and, at day 2 after ischemia, such deficiency augmented upregulation of injury-related genes and worsened histologic injury. Renal HO activity was ma...
Source: Am J Physiol Renal P... - June 19, 2019 Category: Urology & Nephrology Authors: Nath KA, Garovic VD, Grande JP, Croatt AJ, Ackerman AW, Farrugia G, Katusic ZS, Belcher JD, Vercellotti GM Tags: Am J Physiol Renal Physiol Source Type: research

Urinary dysfunction in transgenic sickle cell mice: model of idiopathic overactive bladder syndrome.
In conclusion, our comprehensive behavioral and functional study of the SCD mouse lower genitourinary tract, in correlation with that of the NO deficient mouse, reveals NO effector actions in voiding function and suggests that NO signaling derangements are associated with an OAB phenotype. These findings may allow further study of molecular targets for characterization and evaluation of OAB. PMID: 31215803 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - June 19, 2019 Category: Urology & Nephrology Authors: Karakus S, Anele UA, Silva FH, Musicki B, Burnett AL Tags: Am J Physiol Renal Physiol Source Type: research

Effects of reactive oxygen species on renal tubular transport.
Abstract Reactive oxygen species (ROS) play a critical role in regulating nephron transport both via the transcellular and paracellular pathways under physiological and pathological circumstances. Here we review the progress made in the past ~10 years in understanding how ROS regulate solute and water transport in individual nephron segments. Still our knowledge in this field is rudimentary with basic information lacking. This is most obvious when looking at the reported disparate effects of superoxide (O2-) and hydrogen peroxide (H2O2) on proximal nephron transport, where there are no easy explanations as to how ...
Source: Am J Physiol Renal P... - June 19, 2019 Category: Urology & Nephrology Authors: Gonzalez-Vicente A, Hong NJ, Garvin JL Tags: Am J Physiol Renal Physiol Source Type: research

Urine citrate excretion identifies changes in acid retention as eGFR declines in patients with chronic kidney disease.
We examined as secondary analysis if increased acid (H+) retention occurring as eGFR decreases in patients with chronic kidney disease (CKD) stage 2 eGFR (60-89 ml.min-1.73m-2) (CKD 2) without metabolic acidosis and followed over ten years is predicted by changes in 8 hour urine citrate excretion (UcitrateV). We randomized 120 CKD 2 non-diabetic, hypertension-associated nephropathy patients with plasma total CO2 (PTCO2)> 24 mM to receive 0.5 mEq/kg bw/day NaHCO3 (HCO3-, n=40), 0.5 mEq/kg bw/day NaCl (NaCl, n=40), or Usual Care (UC, n=40). We assessed eGFR (CKD-EPI) and H+ retention by comparing observed to expected PTCO...
Source: Am J Physiol Renal P... - June 19, 2019 Category: Urology & Nephrology Authors: Goraya N, Simoni J, Sager LN, Mamun A, Madias NE, Wesson DE Tags: Am J Physiol Renal Physiol Source Type: research

ANP-stimulated sodium secretion in the collecting duct prevents sodium retention in renal adaptation to acid load.
In conclusion, paracrine stimulation of HKA2 by ANP is responsible for the escape of the sodium retaining effect of aldosterone during metabolic acidosis. PMID: 31188029 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - June 12, 2019 Category: Urology & Nephrology Authors: Cheval L, Bakouh N, Walter C, Tembely D, Morla L, Escher G, Vogt B, Crambert G, Planelles G, Doucet A Tags: Am J Physiol Renal Physiol Source Type: research

Transcriptional profiling of the zebrafish proximal tubule.
Abstract The hepatocyte nuclear factor 1 beta (Hnf1b) transcription factor is a key regulator of kidney tubule formation and is associated with a syndrome of renal cysts and early onset diabetes. To further our understanding of Hnf1b in the developing zebrafish kidney, we have performed RNA-Seq of proximal tubules from hnf1b-deficient larvae. This analysis revealed an enrichment of gene transcripts encoding transporters of the solute carrier (SLC) superfamily, including multiple members of the slc2 and slc5 glucose transporters. An investigation of the expression of slc2a1a, slc2a2, slc5a2, as well as a poorly stu...
Source: Am J Physiol Renal P... - June 12, 2019 Category: Urology & Nephrology Authors: Sander V, Salleh L, Naylor RW, Schierding W, Sontam DM, O'Sullivan JM, Davidson AJ Tags: Am J Physiol Renal Physiol Source Type: research

Detection of cellular hypoxia by pimonidazole adduct immunohistochemistry in kidney disease: Methodological pitfalls and their solution.
Abstract Pimonidazole adduct immunohistochemistry is one of the few available methods for assessing renal tissue hypoxia at the cellular level. It appears to be prone to artifactual false-positive staining under some circumstances. Here, we assessed the nature of this false-positive staining and, having determined how to avoid it, re-examined the nature of cellular hypoxia in rat models of kidney disease. When a mouse-derived anti-pimonidazole primary antibody was used, two types of staining were observed. Firstly, there was diffuse staining of the cytoplasm of tubular epithelial cells which was largely absent whe...
Source: Am J Physiol Renal P... - June 12, 2019 Category: Urology & Nephrology Authors: Ow CPC, Ullah MM, Ngo JP, Sayakkarage A, Evans RG Tags: Am J Physiol Renal Physiol Source Type: research

Renal Iron Accelerates the Progression of Diabetic Nephropathy in HFE (High Fe-iron) Gene Knockout Mouse Model of Iron Overload.
Abstract Diabetic Nephropathy (DN) is the most common cause of end-stage renal disease associated with high mortality worldwide. Increase in iron levels have been reported in the diabetic rat kidneys as well as in the human urine of diabetic patients. In addition, low iron diet or iron chelators delay DN progression in diabetic patients and in animal models of diabetes. Possible maladaptive mechanisms of organ damage by tissue iron accumulation have not been well studied. We recently reported that iron induced retinal renin angiotensin system (RAS) and accelerated the progression of diabetic retinopathy. However, ...
Source: Am J Physiol Renal P... - June 12, 2019 Category: Urology & Nephrology Authors: Chaudhary K, Chilakala A, Ananth S, Mandala A, Veeranan-Karmegam R, Powell FL, Ganapathy V, Gnana-Prakasam JP Tags: Am J Physiol Renal Physiol Source Type: research

Cyclophilin d knockout protects the mouse kidney against cyclosporin a-induced oxidative stress.
CYCLOPHILIN D KNOCKOUT PROTECTS THE MOUSE KIDNEY AGAINST CYCLOSPORIN A-INDUCED OXIDATIVE STRESS. Am J Physiol Renal Physiol. 2019 Jun 12;: Authors: Klawitter J, Klawitter J, Pennington AT, Kirkpatrick B, Roda G, Kotecha NC, Thurman JM, Christians U Abstract Mitochondrial dysfunction and oxidative stress have been implicated in cyclosporin A (CsA)-induced nephrotoxicity. CsA interacts with cyclophilin D (CypD), an essential component of the mitochondrial permeability transition pore (MPTP) and regulator of cell death processes. Controversial reports have suggested that CypD deletion may or may not be p...
Source: Am J Physiol Renal P... - June 12, 2019 Category: Urology & Nephrology Authors: Klawitter J, Klawitter J, Pennington AT, Kirkpatrick B, Roda G, Kotecha NC, Thurman JM, Christians U Tags: Am J Physiol Renal Physiol Source Type: research

Regulation of Renal NaDC1 Expression and Citrate Excretion by NBCe1-A.
This study's purpose was to determine the role of the NBCe1 A variant (NBCe1-A) in citrate metabolism under basal conditions and in response to acid-loading and hypokalemia. NBCe1-A deletion increased citrate excretion and decreased NaDC1 expression in proximal convoluted tubule (PCT) and proximal straight tubule (PST) in medullary ray (PST-MR), but not in PST in outer medulla (PST-OM). Acid-loading wild-type (WT) mice decreased citrate excretion. NaDC1 expression increased only in PCT and PST-MR, and not in PST-MR. In NBCe1-A KO mice, the acid-loading change in citrate excretion was unaffected, changes in PCT NaDC1 expres...
Source: Am J Physiol Renal P... - June 12, 2019 Category: Urology & Nephrology Authors: Osis G, Webster KL, Harris AN, Lee HW, Chen C, Fang L, Romero MF, Khattri RB, Merritt ME, Verlander JW, Weiner ID Tags: Am J Physiol Renal Physiol Source Type: research

Pioglitazone decreased renal calcium oxalate crystal formation by suppressing M1-macrophage polarization via PPAR γ-microRNA-23 axis.
This study had proven that PGZ decreased renal calcium oxalate crystal formation and renal inflammatory injury by suppressing M1Mps polarization through a PPARγ-miR-23-Irf1/Pknox1 axis. PGZ is liable to be a potential therapeutical medicine for treating urolithiasis. PMID: 31091119 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - May 15, 2019 Category: Urology & Nephrology Authors: Chen Z, Yuan P, Sun X, Tang K, Liu H, Han S, Ye T, Liu X, Yang X, Zeng J, Yan L, Xing J, Xiao K, Ye Z, Xu H Tags: Am J Physiol Renal Physiol Source Type: research

Cystitis-induced bladder pain is Toll-like receptor 4 dependent in a transgenic autoimmune cystitis murine model: A MAPP Research Network Animal Study.
In this study, we sought to determine whether altered TLR4 activation plays a role in pelvic/bladder pain seen in IC/BPS patients using our validated IC/BPS-like transgenic autoimmune cystitis model (URO-OVA). URO-OVA mice developed responses consistent with pelvic and bladder pain after cystitis induction, which was associated with increased splenocyte production of TLR4-mediated proinflammatory cytokines interleukin (IL)-1b, IL-6 and tumor necrosis factor (TNF)-a. Increased spinal expression of mRNAs for proinflammatory cytokines IL-6 and TNF-a, glial activation markers CD11b and glial fibrillary acidic protein (GFAP), a...
Source: Am J Physiol Renal P... - May 15, 2019 Category: Urology & Nephrology Authors: Cui X, Jing X, Lutgendorf SK, Bradley CS, Schrepf A, Erickson BA, Magnotta VA, Ness TJ, Kreder KJ, O'Donnell MA, Luo Y Tags: Am J Physiol Renal Physiol Source Type: research

A versatile aquaporin-2 cell system for quantitative temporal expression and live-cell imaging.
In this study we generated and validated a Flp-In T-REx MDCK cell system for temporal and quantitative control of AQP2 and phospho-mimicking mutants. We have verified that expression levels can be temporally and quantitatively controlled and that AQP2 translocated to the plasma membrane in response to elevated cAMP, which also induced S256 phosphorylation. The phospho-mimicking mutants AQP2-S256A and AQP2-S256D localized as previously described primarily intracellular and to the plasma membrane, respectively. Induction of AQP2 expression in combination with transient, low expression of EGFP-AQP2 enabled expression without ...
Source: Am J Physiol Renal P... - May 15, 2019 Category: Urology & Nephrology Authors: Holst MR, Nejsum LN Tags: Am J Physiol Renal Physiol Source Type: research

Renal subcapsular transplantation of hepatocyte growth factor-producing mesothelial cell sheets improves ischemia-reperfusion injury.
We examined the effects of human mesothelial cell (MC) sheets and hepatocyte growth factor (HGF)-transgenic mesothelial cell (HGF-tg MC) sheets transplanted under the renal capsule in an IRI rat model, and compared these two treatments with the intravenous administration of HGF protein and no treatment through serum, histological, and mRNA analyses over 28 days. The MC sheets and HGF-tg MC sheets produced HGF protein and significantly improved acute renal dysfunction, acute tubular necrosis, and survival rate. The improvement in necrosis was likely due to the cell sheets promoting the migration and proliferation of renal t...
Source: Am J Physiol Renal P... - May 15, 2019 Category: Urology & Nephrology Authors: Miyabe Y, Sekiya S, Sugiura N, Oka M, Karasawa K, Moriyama T, Nitta K, Shimizu T Tags: Am J Physiol Renal Physiol Source Type: research

Bile acid signaling in renal water regulation.
Abstract Emerging evidence has shown that bile acids play important roles in renal physiology and diseases by activating two major receptors, the nuclear farnesoid X receptor (FXR) and the membrane G protein-coupled bile acid receptor-1 (Gpbar1, also known as TGR5). Both FXR and TGR5 have been identified in human and rodent kidneys, where they are profoundly involved in renal water handling. In mice, FXR- or TGR5-related gene deficiency has been associated with reduced aquaporin-2 (AQP2) expression accompanied with impaired urinary concentration ability. In this mini-review, we briefly discuss the current understa...
Source: Am J Physiol Renal P... - May 15, 2019 Category: Urology & Nephrology Authors: Li S, Li C, Wang W Tags: Am J Physiol Renal Physiol Source Type: research

Stress granules are formed in renal proximal tubular cells during metabolic stress and ischemic injury for cell survival.
Abstract Stress granule (SG) is a type of cytoplasmic structures formed in eukaryotic cells upon cell stress, which mainly contains RNA-binding proteins and RNAs. The formation of SG is generally regarded as a mechanism for cells to survive a harsh insult. However, little is known about SG formation and function in kidneys. To address this, we applied different kinds of stressors to cultured proximal tubular cells as well as a short period of ischemia/reperfusion to mouse kidneys. It was found that glycolytic inhibitors such as 2DG (2-deoxy-D-glucose) and 3PO (3-(3-pyridinyl)-1-(4-pyridinyl)-2-propen-1-one) induce...
Source: Am J Physiol Renal P... - May 15, 2019 Category: Urology & Nephrology Authors: Wang S, Kwon SH, Su Y, Dong Z Tags: Am J Physiol Renal Physiol Source Type: research

Renal tubular injury exacerbated by Vasohibin-1 deficiency in a murine cisplatin-induced acute kidney injury model.
Abstract Acute kidney injury (AKI) is frequently encountered in clinical practice, particularly secondary to cardiovascular surgery and administration of nephrotoxic agents, and is increasingly recognized for initiating a transition to chronic kidney disease. Clarifying the pathogenesis of AKI could facilitate the development of novel preventive strategies because the occurrence of hospital-acquired AKI is often anticipated. Vasohibin-1 (VASH1) was initially identified as an antiangiogenic factor derived from endothelial cells. VASH1 expression in endothelial cells has subsequently been reported to enhance cellula...
Source: Am J Physiol Renal P... - May 15, 2019 Category: Urology & Nephrology Authors: Tanimura S, Tanabe K, Miyake H, Masuda K, Tsushida K, Morioka T, Sugiyama H, Sato Y, Wada J Tags: Am J Physiol Renal Physiol Source Type: research

MCP-1 Promotes Detrimental Cardiac Physiology, Pulmonary Edema and Death in the cpk model of Polycystic Kidney Disease.
We report that KO of MCP-1 in these mice increased survival. Surprisingly, however, there was no significant difference in renal macrophage concentration, nor was there improvement in cystic disease or kidney function. Examination of cpk mice revealed cardiac hypertrophy, and measurement of ECG parameters revealed cardiac repolarization abnormalities compared to non-cystic mice. MCP-1 KO did not affect the number of cardiac macrophages nor did it alleviate the cardiac aberrancies. However, MCP-1 KO did prevent development of pulmonary edema, which occurred in cpk mice, and promoted a decreased resting heart rate and an inc...
Source: Am J Physiol Renal P... - May 15, 2019 Category: Urology & Nephrology Authors: Salah SM, Meisenheimer JD, Rao R, Peda JD, Wallace DP, Foster D, Li X, Li X, Zhou X, Vallejo JA, Wacker MJ, Fields TA, Swenson Fields KI Tags: Am J Physiol Renal Physiol Source Type: research

Knockout of Na-glucose-cotransporter SGLT1 mitigates diabetes-induced upregulation of nitric oxide synthase-1 in macula densa and glomerular hyperfiltration.
Abstract The Na-glucose-cotransporter SGLT1 mediates glucose reabsorption in late proximal tubules. SGLT1 also mediates macula densa-sensing of an increase in luminal glucose, which increases nitric oxide synthase-1 (MD-NOS1)-mediated NO formation and potentially GFR. Here the contribution of SGLT1 was tested by gene-knockout (-/-) in type 1 diabetic Akita mice. A low-glucose diet was fed to prevent intestinal malabsorption in Sglt1-/- mice and minimize the contribution of intestinal SGLT1. Hyperglycemia was modestly reduced in Sglt1-/- vs littermate wild-type (WT) Akita mice (480 vs. 550mg/dl), associated with re...
Source: Am J Physiol Renal P... - May 15, 2019 Category: Urology & Nephrology Authors: Song P, Huang W, Onishi A, Patel R, Kim YC, van Ginkel C, Fu Y, Freeman B, Koepsell H, Thomson SC, Liu R, Vallon V Tags: Am J Physiol Renal Physiol Source Type: research

Superoxide increases surface nkcc2 in the rat thick ascending limbs via pkc.
SUPEROXIDE INCREASES SURFACE NKCC2 IN THE RAT THICK ASCENDING LIMBS VIA PKC. Am J Physiol Renal Physiol. 2019 May 15;: Authors: Haque MZ, Oritz P Abstract The apical Na/K/2Cl cotransporter NKCC2 mediates NaCl reabsorption by the thick ascending limbs (TAL). The free radical superoxide (O2-) stimulates TAL NaCl absorption by enhancing NKCC2 activity. In contrast, NO scavenges O2- and inhibits NKCC2. NKCC2 activity depends on the number of NKCC2 transporters in the TAL apical membrane and its phosphorylation. We hypothesized that O2- stimulates NKCC2 activity by enhancing apical surface NKCC2 expression...
Source: Am J Physiol Renal P... - May 15, 2019 Category: Urology & Nephrology Authors: Haque MZ, Oritz P Tags: Am J Physiol Renal Physiol Source Type: research

Doxorubicin induces detrusor smooth muscle impairments through myosin dysregulation, leading to a risk of lower urinary tract dysfunction.
In this study, we tested the hypothesis that systemic doxorubicin administration also affects detrusor smooth muscle function in the urinary bladder, especially when administered at a young age. The effects on the detrusor smooth muscle and bladder function were assessed in BALB/cJ mice that received 6 weekly intravenous injections of doxorubicin (3mg/kg/wk) or saline for the control group. Systemic doxorubicin administration resulted in detrusor smooth muscle hypertrophy, increased voiding frequency and a significant attenuation of detrusor smooth muscle contractility followed by a slower relaxation compared to the contro...
Source: Am J Physiol Renal P... - May 8, 2019 Category: Urology & Nephrology Authors: Iguchi N, Dönmez Mİ, Carrasco A, Wilcox DT, Pineda RH, Malykhina AP, Cost NG Tags: Am J Physiol Renal Physiol Source Type: research

Am J Physiol Renal Physiol; +20 new citations
20 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Physiol Renal Physiol These pubmed results were generated on 2019/05/07PubMed comprises more than millions of citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites. (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - May 7, 2019 Category: Urology & Nephrology Tags: Report Source Type: research

NHE8 attenuates calcium influx into NRK cells and the proximal tubule epithelium.
Abstract To garner insight into the renal regulation of calcium homeostasis, we performed an mRNA micro-array on kidneys from mice treated with the calcium sensing receptor (CaSR) agonist cinacalcet. This revealed decreased gene expression of the sodium/proton exchanger isoform 8 (NHE8) in response to CaSR activation. These results were confirmed by quantitative real-time PCR. Moreover, administration of vitamin D also decreased NHE8 mRNA expression. In contrast, renal NHE8 protein expression from the same samples was increased. To examine the role of NHE8 in transmembrane calcium fluxes, we employed the normal ra...
Source: Am J Physiol Renal P... - May 1, 2019 Category: Urology & Nephrology Authors: Wiebe SA, Plain A, Pan W, O'Neill D, Braam B, Alexander RT Tags: Am J Physiol Renal Physiol Source Type: research

A study of sirolimus and an mTOR kinase inhibitor (TORKi) in a hypomorphic Pkd1 mouse model of autosomal dominant polycystic kidney disease (ADPKD).
In conclusion, both torin2 and sirolimus were equally effective in decreasing cyst burden, improving kidney function, and mediated comparable effects on mTORC1 and 2 signaling and proliferation in the Pkd1RC/RC kidney. PMID: 31042058 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - May 1, 2019 Category: Urology & Nephrology Authors: Holditch SJ, Brown CN, Atwood DJ, Lombardi AM, Nguyen KN, Toll HW, Hopp K, Edelstein CL Tags: Am J Physiol Renal Physiol Source Type: research

Early podocyte injury and elevated levels of urinary podocyte-derived extracellular vesicles in swine with metabolic syndrome: Role of podocyte mitochondria.
CONCLUSIONS: Early MetS induces podocyte injury and mitochondrial damage, which can be blunted by mitoprotection. Urinary pEVs reflecting podocyte injury might represent early markers of MetS-related kidney disease and a novel therapeutic target. PMID: 31042059 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - May 1, 2019 Category: Urology & Nephrology Authors: Zhang LH, Zhu XY, Eirin A, Aghajani Nargesi A, Woollard JR, Santelli A, Sun IO, Textor SC, Lerman LO Tags: Am J Physiol Renal Physiol Source Type: research

Deficiency of the T-type calcium channels Cav3.1 and Cav3.2 has no effect on angiotensin II hypertension but differential effect on plasma aldosterone in mice.
In conclusion, T-type channels Cav3.1and Cav3.2do not contribute to baseline blood pressure level and angiotensin II-induced hypertension. Cav3.1, not Cav3.2, contributes to aldosterone secretion. Aldosterone promotes cardiac hypertrophy during hypertension. PMID: 31042060 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - May 1, 2019 Category: Urology & Nephrology Authors: Thuesen AD, Finsen SH, Rasmussen LL, Andersen DC, Jensen BL, Hansen PBL Tags: Am J Physiol Renal Physiol Source Type: research

Characterizing Novel Olfactory Receptors Expressed in the Renal Cortex.
In this study, we cloned and studied 7 renal ORs, which we previously found to be expressed in murine renal cortex. As most ORs are orphan receptors, our goal was to identify ligands for these ORs in the hope that this will guide future research into their functional roles. We identified novel ligands for 2 ORs: Olfr558 and Olfr90. For Olfr558, we confirmed activation by previously reported ligands, and identified 16 additional carboxylic acids that activated this OR. The strongest activation of Olfr558 was produced by butyric, cyclobutanecarboxylic, isovaleric, 2-, 3-, and 4-methylvaleric, and valeric acids. The primary i...
Source: Am J Physiol Renal P... - May 1, 2019 Category: Urology & Nephrology Authors: Halperin Kuhns VL, Sanchez J, Sarver DC, Khalil Z, Rajkumar P, Marr KA, Pluznick JL Tags: Am J Physiol Renal Physiol Source Type: research

Am J Physiol Renal Physiol; +31 new citations
31 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Physiol Renal Physiol These pubmed results were generated on 2019/04/24PubMed comprises more than millions of citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites. (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - April 24, 2019 Category: Urology & Nephrology Tags: Report Source Type: research

Am J Physiol Renal Physiol; +19 new citations
19 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Physiol Renal Physiol These pubmed results were generated on 2019/04/18PubMed comprises more than millions of citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites. (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - April 18, 2019 Category: Urology & Nephrology Tags: Report Source Type: research

MST3 (Mammalian Ste20 Kinase 3) is involved in ENaC-mediated hypertension.
Abstract Liddle syndrome is an inherited form of human hypertension caused by increasing ENaC (epithelial sodium channel) expression. Increased Na+ retention through ENaC with subsequent volume expansion causes hypertension. In addition to ENaC, NKCC (Na-K-Cl cotransporter) and NCC (Na-Cl symporter) are responsible for Na+ reabsorption in the kidneys. Several Na+ transporters are evolutionarily regulated by the Ste20 kinase family. Ste20-related proline/alanine-rich kinase (SPAK) and oxidative stress-responsive kinase-1 (OSR1) phosphorylate downstream NKCC2 and NCC to maintain Na+ and blood pressure (BP) homeostas...
Source: Am J Physiol Renal P... - April 10, 2019 Category: Urology & Nephrology Authors: Lu TJ, Kan WC, Yang SS, Jiang ST, Wu SN, Ling P, Bao BY, Lin CY, Yang ZY, Weng YP, Chan CH, Lu TL Tags: Am J Physiol Renal Physiol Source Type: research

Glycolysis inhibitors suppress renal interstitial fibrosis via divergent effects on fibroblasts and tubular cells.
In this study, we first showed that blockade of glycolysis with either Dichloroacetate (DCA) or shikonin to target different glycolytic enzymes reduced renal fibrosis in the mouse model of unilateral ureteral obstruction (UUO). Both inhibitors evidently suppressed the induction of fibronectin and collagen I in obstructed kidneys with DCA also showed inhibitory effects on collagen IV and a-smooth muscle actin (a-SMA). Histological examination also confirmed less collagen deposition in DCA treated kidneys. Both DCA and shikonin significantly inhibited renal tubular apoptosis but not interstitial apoptosis in UUO. Macrophage ...
Source: Am J Physiol Renal P... - April 10, 2019 Category: Urology & Nephrology Authors: Wei Q, Su J, Dong G, Zhang M, Huo Y, Dong Z Tags: Am J Physiol Renal Physiol Source Type: research

Apparently normal kidney development in mice with conditional disruption of ANGII-AT1 receptor genes in FoxD1 positive stroma cell precursors.
Abstract An intact renin-angiotensin-system involving ANGII-AT1 receptors is crucial for normal kidney development. It is still unclear in which cell types AT1 receptor signaling is required for normal kidney development, maturation and function. Since all kidney cells deriving from stroma progenitor cells express AT1 receptors and since stromal cells fundamentally influence nephrogenesis and tubular maturation, we investigated the relevance of AT1 receptors in stromal progenitors and their descendants for renal development and function. For this aim, we have generated and analyzed mice with a conditional deletion...
Source: Am J Physiol Renal P... - April 10, 2019 Category: Urology & Nephrology Authors: Schrankl J, Neubauer B, Fuchs M, Gerl K, Wagner C, Kurtz A Tags: Am J Physiol Renal Physiol Source Type: research

The Relationship between Maternal Global Nutrient Restriction during Pregnancy and Offspring Kidney Structure and Function:A Systematic Review of Animal studies.
CONCLUSION: The current body of evidence in animals suggests that exposure to maternal global nutrient restriction during pregnancy has detrimental effects on offspring kidney structure and function, such as lower kidney weight, lower nephron endowment, larger glomerular size and lower GFR. Further long-term follow-up of studies in large animal models investigating kidney function through to adulthood are warranted. PMID: 30969805 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - April 10, 2019 Category: Urology & Nephrology Authors: Lee YQ, Beckett EL, Sculley DV, Rae K, Collins CE, Pringle KG Tags: Am J Physiol Renal Physiol Source Type: research

Peptidyl arginine deiminase-4 exacerbates ischemic AKI by finding NEMO (NF κB Essential Modulator).
Peptidyl arginine deiminase-4 exacerbates ischemic AKI by finding NEMO (NFκB Essential Modulator). Am J Physiol Renal Physiol. 2019 Apr 03;: Authors: Rabadi MM, Han SJ, Kim M, D'Agati VD, Lee HT Abstract Peptidyl arginine deiminase-4 (PAD4) catalyzes the conversion of peptidylarginine residues to peptidylcitrulline. We previously showed that kidney ischemia and reperfusion (IR) injury increases renal proximal tubular PAD4 expression and activity. Furthermore, kidney PAD4 plays a critical role in ischemic acute kidney injury (AKI) injury by promoting renal tubular inflammation, neutrophil infiltr...
Source: Am J Physiol Renal P... - April 3, 2019 Category: Urology & Nephrology Authors: Rabadi MM, Han SJ, Kim M, D'Agati VD, Lee HT Tags: Am J Physiol Renal Physiol Source Type: research

Genetic Disruption of Npr1 Depletes T Regulatory Cells and Provokes High Levels of Proinflammatory Cytokines and Fibrosis in the Kidneys of Female Mutant Mice.
Abstract The present study was designed to determine the effects of gene-knockout of guanylyl cyclase/natriuretic peptide receptor-A (GC-A/NPRA) on immunogenic responses affecting kidney function and blood pressure (BP) in Npr1 (coding for GC-A/NPRA) null mutant mice. We used female Npr1 gene-disrupted ( Npr1-/-, 0-copy), heterozygous ( Npr1+/-, 1-copy), wild-type ( Npr1+/+, 2-copy) and gene-duplicated ( Npr1++/++, 4-copy) mice. Expression levels of Toll-like receptor 2/4 (TLR2/TLR4) mRNA were increased 4- to 5-fold in 1-copy mice and 6- to 10-fold in 0-copy mice; protein levels were increased 2.5- to 3-fold in 1-...
Source: Am J Physiol Renal P... - April 3, 2019 Category: Urology & Nephrology Authors: Gogulamudi VR, Mani I, Subramanian U, Pandey KN Tags: Am J Physiol Renal Physiol Source Type: research

Loss of reticulocalbin 2 lowers blood pressure and restrains angiotensin II-induced hypertension in vivo.
Abstract Hypertension affects over one billion people worldwide and increases risk for heart failure, stroke and chronic kidney disease. Despite high prevalence and devastating impact, its etiology still remains poorly understood for most hypertensive cases. Rcn2, encoding reticulocalbin 2, is a candidate gene for atherosclerosis we previously demonstrated in mice. Here we identified Rcn2 as a novel regulator of blood pressure in mice. Rcn2 was dramatically upregulated in arteries undergoing structural remodeling. Deletion of Rcn2 lowered basal blood pressure and attenuated angiotensin II-induced hypertension in C...
Source: Am J Physiol Renal P... - April 3, 2019 Category: Urology & Nephrology Authors: Li J, Cechova S, Wang L, Isakson BE, Le T, Shi W Tags: Am J Physiol Renal Physiol Source Type: research