Predicting Sex Differences in the Effects of Diuretics in Renal Epithelial Transport during Angiotensin II-induced Hypertension
Am J Physiol Renal Physiol. 2024 Mar 14. doi: 10.1152/ajprenal.00398.2023. Online ahead of print.ABSTRACTChronic angiotensin II (AngII) infusion is an experimental model that induces hypertension in rodents. The natriuresis, diuresis, and blood pressure responses differ between males and females. This is perhaps not unexpected, given the rodent kidney, which plays a key role in blood pressure regulation, exhibit marked sex differences. Under normotensive conditions, compared to males, the female rat nephron exhibits lower Na+/H+ exchanger 3 (NHE3) activity along the proximal tubule, but higher Na+ transporter activities al...
Source: Am J Physiol Renal P... - March 14, 2024 Category: Urology & Nephrology Authors: Kaixin Zheng Anita T Layton Source Type: research
Tnik depletion induces inflammation and apoptosis ininjured renal proximal tubule epithelial cells
Am J Physiol Renal Physiol. 2024 Mar 14. doi: 10.1152/ajprenal.00262.2023. Online ahead of print.ABSTRACTIn the aftermath of acute kidney injury (AKI), surviving proximal tubule epithelia repopulate injured tubules to promote repair. However, a portion of cells fail to repair (termed Failed Repair Proximal Tubule Cells, FR-PTCs) and exert ongoing proinflammatory and profibrotic effects. To better understand the molecular drivers of the FR-PTC state, we reanalyzed a mouse ischemia-reperfusion injury single nucleus RNA-sequencing (snRNA-seq) atlas to identify Traf2 and Nck Interacting Kinase, Tnik, to be exclusively expresse...
Source: Am J Physiol Renal P... - March 14, 2024 Category: Urology & Nephrology Authors: Shayna T J Bradford Haojia Wu Yuhei Kirita Changfeng Chen Nicole P Malvin Yasuhiro Yoshimura Yoshiharu Muto Benjamin D Humphreys Source Type: research
Mice with a < em > Pax2 < /em > missense variant display impaired glomerular repair
Am J Physiol Renal Physiol. 2024 Mar 14. doi: 10.1152/ajprenal.00259.2023. Online ahead of print.ABSTRACTPAX2 regulates kidney development and its expression persists in parietal epithelial cells (PECs), potentially serving as a podocyte reserve. We hypothesize that mice with a Pax2 pathogenic missense variant (Pax2A220G/+) have impaired PEC-mediated podocyte regeneration. Embryonic wild type mouse kidneys showed overlapping expression of PAX2/WT-1 until PEC and podocyte differentiation, reflecting a close lineage relationship. Embryonic and adult Pax2A220G/+ mice have reduced nephron number but demonstrated no glomerular ...
Source: Am J Physiol Renal P... - March 14, 2024 Category: Urology & Nephrology Authors: Joanna Cunanan Sarada Sriya Rajyam Bedra Sharif Khalil Udwan Akanchaya Rana Vanessa De Gregorio Samantha Ricardo Andrew Elia Brian Brooks Astrid Weins Martin Pollak Rohan John Moumita Barua Source Type: research
Quantitative phenotyping of < em > Nphs1 < /em > knockout mice as a prerequisite for gene replacement studies
Am J Physiol Renal Physiol. 2024 Mar 14. doi: 10.1152/ajprenal.00412.2023. Online ahead of print.ABSTRACTSteroid-resistant nephrotic (SRNS) syndrome is the second most frequent cause of chronic kidney disease before the age of 25 years. Nephrin, encoded by NPHS1, localizes to the slit diaphragm of glomerular podocytes and is the predominant structural component of the glomerular filtration barrier. Biallelic variants in NPHS1 can cause congenital nephrotic syndrome of the Finnish type (CNS-1) for which, to date, no causative therapy is available. Recently, adeno-associated virus (AAV) vectors targeting the glomerular podoc...
Source: Am J Physiol Renal P... - March 14, 2024 Category: Urology & Nephrology Authors: Florian Buerger Lea M Merz Ken Saida Seyoung Yu Daanya Salmanullah Katharina Lemberg Nils D Mertens Bshara Mansour Caroline M Kolvenbach Kirollos Yousef Alina Braun Gijs A C Franken Nicole Endlich Ronen Schneider Shrilee Shril Friedhelm Hildebrandt Source Type: research
Predicting Sex Differences in the Effects of Diuretics in Renal Epithelial Transport during Angiotensin II-induced Hypertension
Am J Physiol Renal Physiol. 2024 Mar 14. doi: 10.1152/ajprenal.00398.2023. Online ahead of print.ABSTRACTChronic angiotensin II (AngII) infusion is an experimental model that induces hypertension in rodents. The natriuresis, diuresis, and blood pressure responses differ between males and females. This is perhaps not unexpected, given the rodent kidney, which plays a key role in blood pressure regulation, exhibit marked sex differences. Under normotensive conditions, compared to males, the female rat nephron exhibits lower Na+/H+ exchanger 3 (NHE3) activity along the proximal tubule, but higher Na+ transporter activities al...
Source: Am J Physiol Renal P... - March 14, 2024 Category: Urology & Nephrology Authors: Kaixin Zheng Anita T Layton Source Type: research
Tnik depletion induces inflammation and apoptosis ininjured renal proximal tubule epithelial cells
Am J Physiol Renal Physiol. 2024 Mar 14. doi: 10.1152/ajprenal.00262.2023. Online ahead of print.ABSTRACTIn the aftermath of acute kidney injury (AKI), surviving proximal tubule epithelia repopulate injured tubules to promote repair. However, a portion of cells fail to repair (termed Failed Repair Proximal Tubule Cells, FR-PTCs) and exert ongoing proinflammatory and profibrotic effects. To better understand the molecular drivers of the FR-PTC state, we reanalyzed a mouse ischemia-reperfusion injury single nucleus RNA-sequencing (snRNA-seq) atlas to identify Traf2 and Nck Interacting Kinase, Tnik, to be exclusively expresse...
Source: Am J Physiol Renal P... - March 14, 2024 Category: Urology & Nephrology Authors: Shayna T J Bradford Haojia Wu Yuhei Kirita Changfeng Chen Nicole P Malvin Yasuhiro Yoshimura Yoshiharu Muto Benjamin D Humphreys Source Type: research
Mice with a < em > Pax2 < /em > missense variant display impaired glomerular repair
Am J Physiol Renal Physiol. 2024 Mar 14. doi: 10.1152/ajprenal.00259.2023. Online ahead of print.ABSTRACTPAX2 regulates kidney development and its expression persists in parietal epithelial cells (PECs), potentially serving as a podocyte reserve. We hypothesize that mice with a Pax2 pathogenic missense variant (Pax2A220G/+) have impaired PEC-mediated podocyte regeneration. Embryonic wild type mouse kidneys showed overlapping expression of PAX2/WT-1 until PEC and podocyte differentiation, reflecting a close lineage relationship. Embryonic and adult Pax2A220G/+ mice have reduced nephron number but demonstrated no glomerular ...
Source: Am J Physiol Renal P... - March 14, 2024 Category: Urology & Nephrology Authors: Joanna Cunanan Sarada Sriya Rajyam Bedra Sharif Khalil Udwan Akanchaya Rana Vanessa De Gregorio Samantha Ricardo Andrew Elia Brian Brooks Astrid Weins Martin Pollak Rohan John Moumita Barua Source Type: research
Quantitative phenotyping of < em > Nphs1 < /em > knockout mice as a prerequisite for gene replacement studies
Am J Physiol Renal Physiol. 2024 Mar 14. doi: 10.1152/ajprenal.00412.2023. Online ahead of print.ABSTRACTSteroid-resistant nephrotic (SRNS) syndrome is the second most frequent cause of chronic kidney disease before the age of 25 years. Nephrin, encoded by NPHS1, localizes to the slit diaphragm of glomerular podocytes and is the predominant structural component of the glomerular filtration barrier. Biallelic variants in NPHS1 can cause congenital nephrotic syndrome of the Finnish type (CNS-1) for which, to date, no causative therapy is available. Recently, adeno-associated virus (AAV) vectors targeting the glomerular podoc...
Source: Am J Physiol Renal P... - March 14, 2024 Category: Urology & Nephrology Authors: Florian Buerger Lea M Merz Ken Saida Seyoung Yu Daanya Salmanullah Katharina Lemberg Nils D Mertens Bshara Mansour Caroline M Kolvenbach Kirollos Yousef Alina Braun Gijs A C Franken Nicole Endlich Ronen Schneider Shrilee Shril Friedhelm Hildebrandt Source Type: research
Predicting Sex Differences in the Effects of Diuretics in Renal Epithelial Transport during Angiotensin II-induced Hypertension
Am J Physiol Renal Physiol. 2024 Mar 14. doi: 10.1152/ajprenal.00398.2023. Online ahead of print.ABSTRACTChronic angiotensin II (AngII) infusion is an experimental model that induces hypertension in rodents. The natriuresis, diuresis, and blood pressure responses differ between males and females. This is perhaps not unexpected, given the rodent kidney, which plays a key role in blood pressure regulation, exhibit marked sex differences. Under normotensive conditions, compared to males, the female rat nephron exhibits lower Na+/H+ exchanger 3 (NHE3) activity along the proximal tubule, but higher Na+ transporter activities al...
Source: Am J Physiol Renal P... - March 14, 2024 Category: Urology & Nephrology Authors: Kaixin Zheng Anita T Layton Source Type: research
Tnik depletion induces inflammation and apoptosis ininjured renal proximal tubule epithelial cells
Am J Physiol Renal Physiol. 2024 Mar 14. doi: 10.1152/ajprenal.00262.2023. Online ahead of print.ABSTRACTIn the aftermath of acute kidney injury (AKI), surviving proximal tubule epithelia repopulate injured tubules to promote repair. However, a portion of cells fail to repair (termed Failed Repair Proximal Tubule Cells, FR-PTCs) and exert ongoing proinflammatory and profibrotic effects. To better understand the molecular drivers of the FR-PTC state, we reanalyzed a mouse ischemia-reperfusion injury single nucleus RNA-sequencing (snRNA-seq) atlas to identify Traf2 and Nck Interacting Kinase, Tnik, to be exclusively expresse...
Source: Am J Physiol Renal P... - March 14, 2024 Category: Urology & Nephrology Authors: Shayna T J Bradford Haojia Wu Yuhei Kirita Changfeng Chen Nicole P Malvin Yasuhiro Yoshimura Yoshiharu Muto Benjamin D Humphreys Source Type: research
Mice with a < em > Pax2 < /em > missense variant display impaired glomerular repair
Am J Physiol Renal Physiol. 2024 Mar 14. doi: 10.1152/ajprenal.00259.2023. Online ahead of print.ABSTRACTPAX2 regulates kidney development and its expression persists in parietal epithelial cells (PECs), potentially serving as a podocyte reserve. We hypothesize that mice with a Pax2 pathogenic missense variant (Pax2A220G/+) have impaired PEC-mediated podocyte regeneration. Embryonic wild type mouse kidneys showed overlapping expression of PAX2/WT-1 until PEC and podocyte differentiation, reflecting a close lineage relationship. Embryonic and adult Pax2A220G/+ mice have reduced nephron number but demonstrated no glomerular ...
Source: Am J Physiol Renal P... - March 14, 2024 Category: Urology & Nephrology Authors: Joanna Cunanan Sarada Sriya Rajyam Bedra Sharif Khalil Udwan Akanchaya Rana Vanessa De Gregorio Samantha Ricardo Andrew Elia Brian Brooks Astrid Weins Martin Pollak Rohan John Moumita Barua Source Type: research
Quantitative phenotyping of < em > Nphs1 < /em > knockout mice as a prerequisite for gene replacement studies
Am J Physiol Renal Physiol. 2024 Mar 14. doi: 10.1152/ajprenal.00412.2023. Online ahead of print.ABSTRACTSteroid-resistant nephrotic (SRNS) syndrome is the second most frequent cause of chronic kidney disease before the age of 25 years. Nephrin, encoded by NPHS1, localizes to the slit diaphragm of glomerular podocytes and is the predominant structural component of the glomerular filtration barrier. Biallelic variants in NPHS1 can cause congenital nephrotic syndrome of the Finnish type (CNS-1) for which, to date, no causative therapy is available. Recently, adeno-associated virus (AAV) vectors targeting the glomerular podoc...
Source: Am J Physiol Renal P... - March 14, 2024 Category: Urology & Nephrology Authors: Florian Buerger Lea M Merz Ken Saida Seyoung Yu Daanya Salmanullah Katharina Lemberg Nils D Mertens Bshara Mansour Caroline M Kolvenbach Kirollos Yousef Alina Braun Gijs A C Franken Nicole Endlich Ronen Schneider Shrilee Shril Friedhelm Hildebrandt Source Type: research
Predicting Sex Differences in the Effects of Diuretics in Renal Epithelial Transport during Angiotensin II-induced Hypertension
Am J Physiol Renal Physiol. 2024 Mar 14. doi: 10.1152/ajprenal.00398.2023. Online ahead of print.ABSTRACTChronic angiotensin II (AngII) infusion is an experimental model that induces hypertension in rodents. The natriuresis, diuresis, and blood pressure responses differ between males and females. This is perhaps not unexpected, given the rodent kidney, which plays a key role in blood pressure regulation, exhibit marked sex differences. Under normotensive conditions, compared to males, the female rat nephron exhibits lower Na+/H+ exchanger 3 (NHE3) activity along the proximal tubule, but higher Na+ transporter activities al...
Source: Am J Physiol Renal P... - March 14, 2024 Category: Urology & Nephrology Authors: Kaixin Zheng Anita T Layton Source Type: research
Tnik depletion induces inflammation and apoptosis ininjured renal proximal tubule epithelial cells
Am J Physiol Renal Physiol. 2024 Mar 14. doi: 10.1152/ajprenal.00262.2023. Online ahead of print.ABSTRACTIn the aftermath of acute kidney injury (AKI), surviving proximal tubule epithelia repopulate injured tubules to promote repair. However, a portion of cells fail to repair (termed Failed Repair Proximal Tubule Cells, FR-PTCs) and exert ongoing proinflammatory and profibrotic effects. To better understand the molecular drivers of the FR-PTC state, we reanalyzed a mouse ischemia-reperfusion injury single nucleus RNA-sequencing (snRNA-seq) atlas to identify Traf2 and Nck Interacting Kinase, Tnik, to be exclusively expresse...
Source: Am J Physiol Renal P... - March 14, 2024 Category: Urology & Nephrology Authors: Shayna T J Bradford Haojia Wu Yuhei Kirita Changfeng Chen Nicole P Malvin Yasuhiro Yoshimura Yoshiharu Muto Benjamin D Humphreys Source Type: research
Mice with a < em > Pax2 < /em > missense variant display impaired glomerular repair
Am J Physiol Renal Physiol. 2024 Mar 14. doi: 10.1152/ajprenal.00259.2023. Online ahead of print.ABSTRACTPAX2 regulates kidney development and its expression persists in parietal epithelial cells (PECs), potentially serving as a podocyte reserve. We hypothesize that mice with a Pax2 pathogenic missense variant (Pax2A220G/+) have impaired PEC-mediated podocyte regeneration. Embryonic wild type mouse kidneys showed overlapping expression of PAX2/WT-1 until PEC and podocyte differentiation, reflecting a close lineage relationship. Embryonic and adult Pax2A220G/+ mice have reduced nephron number but demonstrated no glomerular ...
Source: Am J Physiol Renal P... - March 14, 2024 Category: Urology & Nephrology Authors: Joanna Cunanan Sarada Sriya Rajyam Bedra Sharif Khalil Udwan Akanchaya Rana Vanessa De Gregorio Samantha Ricardo Andrew Elia Brian Brooks Astrid Weins Martin Pollak Rohan John Moumita Barua Source Type: research
