Development of Multiplex Molecular Assays for Simultaneous Detection of Dengue Serotypes and Chikungunya Virus for Arbovirus Surveillance
Curr Issues Mol Biol. 2024 Mar 6;46(3):2093-2104. doi: 10.3390/cimb46030134.ABSTRACTThe major arboviruses mainly belong to the Bunyaviridae, Togaviridae, and Flaviviridae families, among which the chikungunya virus and dengue virus have emerged as global public health problems. The main objective of this study was to develop specific, sensitive, and cost-effective molecular multiplex RT-PCR and RT-qPCR assays for the rapid and simultaneous detection of CHIKV and the four serotypes of DENV for arbovirus surveillance. Specific primers for all viruses were designed, and one-step multiplex RT-PCR (mRT-PCR) and RT-qPCR (mRT-qPC...
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Louis Robert W Belem Sylvester Agha Ibemgbo Michel Kir éopori Gomgnimbou Dileep Kumar Verma Antoinette Kabor é Ankit Kumar Ibrahim Sangar é Sujatha Sunil Source Type: research
Relationship between air temperature and dengue incidence: time series study in Minas Gerais, Brazil (2010-2019)
Cad Saude Publica. 2024 Mar 25;40(3):e00076723. doi: 10.1590/0102-311XPT076723. eCollection 2024.ABSTRACTAir temperature is a climatic factor that affects the incidence of dengue, with effects varying according to time and space. We investigated the relationship between minimum air temperature and dengue incidence in Minas Gerais, Brazil, and evaluated the influence of socioeconomic and geographic variables on this relationship. This is a time series study with analysis conducted in three distinct stages: modeling using a distributed lag non-linear model, meta-analysis of models obtained, and meta-regression with geographi...
Source: Cadernos de Saude Publica - March 27, 2024 Category: International Medicine & Public Health Authors: Jo ão Pedro Medeiros Gomes Igor Magaton Ribas Pedro Augusto Rosa Valadares Lucas Santos Jardim M ário Círio Nogueira C ássia de Castro Martins Ferreira Aripuan ã Sakurada Aranha Watanabe Let ícia de Castro Martins Ferreira Source Type: research
Development of Multiplex Molecular Assays for Simultaneous Detection of Dengue Serotypes and Chikungunya Virus for Arbovirus Surveillance
Curr Issues Mol Biol. 2024 Mar 6;46(3):2093-2104. doi: 10.3390/cimb46030134.ABSTRACTThe major arboviruses mainly belong to the Bunyaviridae, Togaviridae, and Flaviviridae families, among which the chikungunya virus and dengue virus have emerged as global public health problems. The main objective of this study was to develop specific, sensitive, and cost-effective molecular multiplex RT-PCR and RT-qPCR assays for the rapid and simultaneous detection of CHIKV and the four serotypes of DENV for arbovirus surveillance. Specific primers for all viruses were designed, and one-step multiplex RT-PCR (mRT-PCR) and RT-qPCR (mRT-qPC...
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Louis Robert W Belem Sylvester Agha Ibemgbo Michel Kir éopori Gomgnimbou Dileep Kumar Verma Antoinette Kabor é Ankit Kumar Ibrahim Sangar é Sujatha Sunil Source Type: research
Relationship between air temperature and dengue incidence: time series study in Minas Gerais, Brazil (2010-2019)
Cad Saude Publica. 2024 Mar 25;40(3):e00076723. doi: 10.1590/0102-311XPT076723. eCollection 2024.ABSTRACTAir temperature is a climatic factor that affects the incidence of dengue, with effects varying according to time and space. We investigated the relationship between minimum air temperature and dengue incidence in Minas Gerais, Brazil, and evaluated the influence of socioeconomic and geographic variables on this relationship. This is a time series study with analysis conducted in three distinct stages: modeling using a distributed lag non-linear model, meta-analysis of models obtained, and meta-regression with geographi...
Source: Cadernos de Saude Publica - March 27, 2024 Category: International Medicine & Public Health Authors: Jo ão Pedro Medeiros Gomes Igor Magaton Ribas Pedro Augusto Rosa Valadares Lucas Santos Jardim M ário Círio Nogueira C ássia de Castro Martins Ferreira Aripuan ã Sakurada Aranha Watanabe Let ícia de Castro Martins Ferreira Source Type: research
Development of Multiplex Molecular Assays for Simultaneous Detection of Dengue Serotypes and Chikungunya Virus for Arbovirus Surveillance
Curr Issues Mol Biol. 2024 Mar 6;46(3):2093-2104. doi: 10.3390/cimb46030134.ABSTRACTThe major arboviruses mainly belong to the Bunyaviridae, Togaviridae, and Flaviviridae families, among which the chikungunya virus and dengue virus have emerged as global public health problems. The main objective of this study was to develop specific, sensitive, and cost-effective molecular multiplex RT-PCR and RT-qPCR assays for the rapid and simultaneous detection of CHIKV and the four serotypes of DENV for arbovirus surveillance. Specific primers for all viruses were designed, and one-step multiplex RT-PCR (mRT-PCR) and RT-qPCR (mRT-qPC...
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Louis Robert W Belem Sylvester Agha Ibemgbo Michel Kir éopori Gomgnimbou Dileep Kumar Verma Antoinette Kabor é Ankit Kumar Ibrahim Sangar é Sujatha Sunil Source Type: research
Endothelial and inflammatory pathophysiology in dengue shock: New insights from a prospective cohort study in Vietnam
by Angela McBride, Huynh Thi Le Duyen, Nguyen Lam Vuong, Phan Vinh Tho, Luong Thi Hue Tai, Nguyen Thanh Phong, Nguyen Thanh Ngoc, Lam Minh Yen, Phung Tran Huy Nhat, Tran Thuy Vi, Martin J. Llewelyn, Louise Thwaites, Nguyen Van Hao, Sophie Yacoub
Dengue shock (DS) is the most severe complication of dengue infection; endothelial hyperpermeability leads to profound plasma leakage, hypovolaemia and extravascular fluid accumulation. At present, the only treatment is supportive with intravenous fluid, but targeted endothelial stabilising therapies and host immune modulators are needed. With the aim of prioritising potential the...
Source: PLoS Neglected Tropical Diseases - March 27, 2024 Category: Tropical Medicine Authors: Angela McBride Source Type: research
Potential use of proprotein convertase subtilisin/kexin type 9 (PCSK9) inhibition and prevention method in viral infection
Microb Cell Fact. 2024 Mar 25;23(1):90. doi: 10.1186/s12934-024-02355-8.ABSTRACTCellular lipid membranes serve as the primary barrier preventing viral infection of the host cell and provide viruses with a critical initial point of contact. Occasionally, viruses can utilize lipids as viral receptors. Viruses depend significantly on lipid rafts for infection at virtually every stage of their life cycle. The pivotal role that proprotein convertase subtilisin/kexin Type 9 (PCSK9) plays in cholesterol homeostasis and atherosclerosis, primarily by post-transcriptionally regulating hepatic low-density lipoprotein receptor (LDLR) ...
Source: Atherosclerosis - March 26, 2024 Category: Cardiology Authors: Khursheed Muzammil Mohammad Hosseini Hooshiar Shirin Varmazyar Thabit Moath Omar Manal Morad Karim Sadeq Aadi Shaylan Kalavi Saman Yasamineh Source Type: research
Potential use of proprotein convertase subtilisin/kexin type 9 (PCSK9) inhibition and prevention method in viral infection
Microb Cell Fact. 2024 Mar 25;23(1):90. doi: 10.1186/s12934-024-02355-8.ABSTRACTCellular lipid membranes serve as the primary barrier preventing viral infection of the host cell and provide viruses with a critical initial point of contact. Occasionally, viruses can utilize lipids as viral receptors. Viruses depend significantly on lipid rafts for infection at virtually every stage of their life cycle. The pivotal role that proprotein convertase subtilisin/kexin Type 9 (PCSK9) plays in cholesterol homeostasis and atherosclerosis, primarily by post-transcriptionally regulating hepatic low-density lipoprotein receptor (LDLR) ...
Source: Atherosclerosis - March 26, 2024 Category: Cardiology Authors: Khursheed Muzammil Mohammad Hosseini Hooshiar Shirin Varmazyar Thabit Moath Omar Manal Morad Karim Sadeq Aadi Shaylan Kalavi Saman Yasamineh Source Type: research
First-in-human trial evaluating safety and pharmacokinetics of AT-752, a novel nucleotide prodrug with pan-serotype activity against dengue virus
Antimicrob Agents Chemother. 2024 Mar 25:e0161523. doi: 10.1128/aac.01615-23. Online ahead of print.ABSTRACTAT-752 is a novel guanosine nucleotide prodrug inhibitor of the dengue virus (DENV) polymerase with sub-micromolar, pan-serotype antiviral activity. This phase 1, double-blind, placebo-controlled, first-in-human study evaluated the safety, tolerability, and pharmacokinetics of ascending single and multiple oral doses of AT-752 in healthy subjects. AT-752 was well tolerated when administered as a single dose up to 1,500 mg or when administered as multiple doses up to 750 mg three times daily (TID). No serious adverse ...
Source: Antimicrobial Agents and Chemotherapy - March 25, 2024 Category: Microbiology Authors: Xiao-Jian Zhou Jason Lickliter Maureen Montrond Laura Ishak Keith Pietropaolo Dayle James Bruce Belanger Arantxa Horga Janet Hammond Source Type: research
Reemergence of Sylvatic Dengue Virus Serotype 2 in Kedougou, Senegal, 2020
Emerg Infect Dis. 2024 Apr;30(4):770-774. doi: 10.3201/eid3004.231301.ABSTRACTIn 2020, a sylvatic dengue virus serotype 2 infection outbreak resulted in 59 confirmed dengue cases in Kedougou, Senegal, suggesting those strains might not require adaptation to reemerge into urban transmission cycles. Large-scale genomic surveillance and updated molecular diagnostic tools are needed to effectively prevent dengue virus infections in Senegal.PMID:38526209 | DOI:10.3201/eid3004.231301 (Source: Emerging Infectious Diseases)
Source: Emerging Infectious Diseases - March 25, 2024 Category: Infectious Diseases Authors: Idrissa Dieng Maryam Diarra Bacary Djilocalisse Sadio Bocar Sow Alioune Gaye Amadou Diallo Martin Faye Marie Henriette Dior Ndione Diawo Diallo Safietou Sankhe Mignane Ndiaye Fode Danfakha Boly Diop Amadou Alpha Sall Gamou Fall Oumar Faye Cheikh Loucoubar Source Type: research
First-in-human trial evaluating safety and pharmacokinetics of AT-752, a novel nucleotide prodrug with pan-serotype activity against dengue virus
Antimicrob Agents Chemother. 2024 Mar 25:e0161523. doi: 10.1128/aac.01615-23. Online ahead of print.ABSTRACTAT-752 is a novel guanosine nucleotide prodrug inhibitor of the dengue virus (DENV) polymerase with sub-micromolar, pan-serotype antiviral activity. This phase 1, double-blind, placebo-controlled, first-in-human study evaluated the safety, tolerability, and pharmacokinetics of ascending single and multiple oral doses of AT-752 in healthy subjects. AT-752 was well tolerated when administered as a single dose up to 1,500 mg or when administered as multiple doses up to 750 mg three times daily (TID). No serious adverse ...
Source: Antimicrobial Agents and Chemotherapy - March 25, 2024 Category: Microbiology Authors: Xiao-Jian Zhou Jason Lickliter Maureen Montrond Laura Ishak Keith Pietropaolo Dayle James Bruce Belanger Arantxa Horga Janet Hammond Source Type: research
Reemergence of Sylvatic Dengue Virus Serotype 2 in Kedougou, Senegal, 2020
Emerg Infect Dis. 2024 Apr;30(4):770-774. doi: 10.3201/eid3004.231301.ABSTRACTIn 2020, a sylvatic dengue virus serotype 2 infection outbreak resulted in 59 confirmed dengue cases in Kedougou, Senegal, suggesting those strains might not require adaptation to reemerge into urban transmission cycles. Large-scale genomic surveillance and updated molecular diagnostic tools are needed to effectively prevent dengue virus infections in Senegal.PMID:38526209 | DOI:10.3201/eid3004.231301 (Source: Emerging Infectious Diseases)
Source: Emerging Infectious Diseases - March 25, 2024 Category: Infectious Diseases Authors: Idrissa Dieng Maryam Diarra Bacary Djilocalisse Sadio Bocar Sow Alioune Gaye Amadou Diallo Martin Faye Marie Henriette Dior Ndione Diawo Diallo Safietou Sankhe Mignane Ndiaye Fode Danfakha Boly Diop Amadou Alpha Sall Gamou Fall Oumar Faye Cheikh Loucoubar Source Type: research
First-in-human trial evaluating safety and pharmacokinetics of AT-752, a novel nucleotide prodrug with pan-serotype activity against dengue virus
Antimicrob Agents Chemother. 2024 Mar 25:e0161523. doi: 10.1128/aac.01615-23. Online ahead of print.ABSTRACTAT-752 is a novel guanosine nucleotide prodrug inhibitor of the dengue virus (DENV) polymerase with sub-micromolar, pan-serotype antiviral activity. This phase 1, double-blind, placebo-controlled, first-in-human study evaluated the safety, tolerability, and pharmacokinetics of ascending single and multiple oral doses of AT-752 in healthy subjects. AT-752 was well tolerated when administered as a single dose up to 1,500 mg or when administered as multiple doses up to 750 mg three times daily (TID). No serious adverse ...
Source: Antimicrobial Agents and Chemotherapy - March 25, 2024 Category: Microbiology Authors: Xiao-Jian Zhou Jason Lickliter Maureen Montrond Laura Ishak Keith Pietropaolo Dayle James Bruce Belanger Arantxa Horga Janet Hammond Source Type: research
Reemergence of Sylvatic Dengue Virus Serotype 2 in Kedougou, Senegal, 2020
Emerg Infect Dis. 2024 Apr;30(4):770-774. doi: 10.3201/eid3004.231301.ABSTRACTIn 2020, a sylvatic dengue virus serotype 2 infection outbreak resulted in 59 confirmed dengue cases in Kedougou, Senegal, suggesting those strains might not require adaptation to reemerge into urban transmission cycles. Large-scale genomic surveillance and updated molecular diagnostic tools are needed to effectively prevent dengue virus infections in Senegal.PMID:38526209 | DOI:10.3201/eid3004.231301 (Source: Emerging Infectious Diseases)
Source: Emerging Infectious Diseases - March 25, 2024 Category: Infectious Diseases Authors: Idrissa Dieng Maryam Diarra Bacary Djilocalisse Sadio Bocar Sow Alioune Gaye Amadou Diallo Martin Faye Marie Henriette Dior Ndione Diawo Diallo Safietou Sankhe Mignane Ndiaye Fode Danfakha Boly Diop Amadou Alpha Sall Gamou Fall Oumar Faye Cheikh Loucoubar Source Type: research
Reemergence of Sylvatic Dengue Virus Serotype 2 in Kedougou, Senegal, 2020
Emerg Infect Dis. 2024 Apr;30(4):770-774. doi: 10.3201/eid3004.231301.ABSTRACTIn 2020, a sylvatic dengue virus serotype 2 infection outbreak resulted in 59 confirmed dengue cases in Kedougou, Senegal, suggesting those strains might not require adaptation to reemerge into urban transmission cycles. Large-scale genomic surveillance and updated molecular diagnostic tools are needed to effectively prevent dengue virus infections in Senegal.PMID:38526209 | PMC:PMC10977847 | DOI:10.3201/eid3004.231301 (Source: Emerging Infectious Diseases)
Source: Emerging Infectious Diseases - March 25, 2024 Category: Infectious Diseases Authors: Idrissa Dieng Maryam Diarra Bacary Djilocalisse Sadio Bocar Sow Alioune Gaye Amadou Diallo Martin Faye Marie Henriette Dior Ndione Diawo Diallo Safietou Sankhe Mignane Ndiaye Fode Danfakha Boly Diop Amadou Alpha Sall Gamou Fall Oumar Faye Cheikh Loucoubar Source Type: research
