Development of Multiplex Molecular Assays for Simultaneous Detection of Dengue Serotypes and Chikungunya Virus for Arbovirus Surveillance
Curr Issues Mol Biol. 2024 Mar 6;46(3):2093-2104. doi: 10.3390/cimb46030134.ABSTRACTThe major arboviruses mainly belong to the Bunyaviridae, Togaviridae, and Flaviviridae families, among which the chikungunya virus and dengue virus have emerged as global public health problems. The main objective of this study was to develop specific, sensitive, and cost-effective molecular multiplex RT-PCR and RT-qPCR assays for the rapid and simultaneous detection of CHIKV and the four serotypes of DENV for arbovirus surveillance. Specific primers for all viruses were designed, and one-step multiplex RT-PCR (mRT-PCR) and RT-qPCR (mRT-qPC...
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Louis Robert W Belem Sylvester Agha Ibemgbo Michel Kir éopori Gomgnimbou Dileep Kumar Verma Antoinette Kabor é Ankit Kumar Ibrahim Sangar é Sujatha Sunil Source Type: research
Relationship between air temperature and dengue incidence: time series study in Minas Gerais, Brazil (2010-2019)
Cad Saude Publica. 2024 Mar 25;40(3):e00076723. doi: 10.1590/0102-311XPT076723. eCollection 2024.ABSTRACTAir temperature is a climatic factor that affects the incidence of dengue, with effects varying according to time and space. We investigated the relationship between minimum air temperature and dengue incidence in Minas Gerais, Brazil, and evaluated the influence of socioeconomic and geographic variables on this relationship. This is a time series study with analysis conducted in three distinct stages: modeling using a distributed lag non-linear model, meta-analysis of models obtained, and meta-regression with geographi...
Source: Cadernos de Saude Publica - March 27, 2024 Category: International Medicine & Public Health Authors: Jo ão Pedro Medeiros Gomes Igor Magaton Ribas Pedro Augusto Rosa Valadares Lucas Santos Jardim M ário Círio Nogueira C ássia de Castro Martins Ferreira Aripuan ã Sakurada Aranha Watanabe Let ícia de Castro Martins Ferreira Source Type: research
Development of Multiplex Molecular Assays for Simultaneous Detection of Dengue Serotypes and Chikungunya Virus for Arbovirus Surveillance
Curr Issues Mol Biol. 2024 Mar 6;46(3):2093-2104. doi: 10.3390/cimb46030134.ABSTRACTThe major arboviruses mainly belong to the Bunyaviridae, Togaviridae, and Flaviviridae families, among which the chikungunya virus and dengue virus have emerged as global public health problems. The main objective of this study was to develop specific, sensitive, and cost-effective molecular multiplex RT-PCR and RT-qPCR assays for the rapid and simultaneous detection of CHIKV and the four serotypes of DENV for arbovirus surveillance. Specific primers for all viruses were designed, and one-step multiplex RT-PCR (mRT-PCR) and RT-qPCR (mRT-qPC...
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Louis Robert W Belem Sylvester Agha Ibemgbo Michel Kir éopori Gomgnimbou Dileep Kumar Verma Antoinette Kabor é Ankit Kumar Ibrahim Sangar é Sujatha Sunil Source Type: research
Relationship between air temperature and dengue incidence: time series study in Minas Gerais, Brazil (2010-2019)
Cad Saude Publica. 2024 Mar 25;40(3):e00076723. doi: 10.1590/0102-311XPT076723. eCollection 2024.ABSTRACTAir temperature is a climatic factor that affects the incidence of dengue, with effects varying according to time and space. We investigated the relationship between minimum air temperature and dengue incidence in Minas Gerais, Brazil, and evaluated the influence of socioeconomic and geographic variables on this relationship. This is a time series study with analysis conducted in three distinct stages: modeling using a distributed lag non-linear model, meta-analysis of models obtained, and meta-regression with geographi...
Source: Cadernos de Saude Publica - March 27, 2024 Category: International Medicine & Public Health Authors: Jo ão Pedro Medeiros Gomes Igor Magaton Ribas Pedro Augusto Rosa Valadares Lucas Santos Jardim M ário Círio Nogueira C ássia de Castro Martins Ferreira Aripuan ã Sakurada Aranha Watanabe Let ícia de Castro Martins Ferreira Source Type: research
Development of Multiplex Molecular Assays for Simultaneous Detection of Dengue Serotypes and Chikungunya Virus for Arbovirus Surveillance
Curr Issues Mol Biol. 2024 Mar 6;46(3):2093-2104. doi: 10.3390/cimb46030134.ABSTRACTThe major arboviruses mainly belong to the Bunyaviridae, Togaviridae, and Flaviviridae families, among which the chikungunya virus and dengue virus have emerged as global public health problems. The main objective of this study was to develop specific, sensitive, and cost-effective molecular multiplex RT-PCR and RT-qPCR assays for the rapid and simultaneous detection of CHIKV and the four serotypes of DENV for arbovirus surveillance. Specific primers for all viruses were designed, and one-step multiplex RT-PCR (mRT-PCR) and RT-qPCR (mRT-qPC...
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Louis Robert W Belem Sylvester Agha Ibemgbo Michel Kir éopori Gomgnimbou Dileep Kumar Verma Antoinette Kabor é Ankit Kumar Ibrahim Sangar é Sujatha Sunil Source Type: research
Relationship between air temperature and dengue incidence: time series study in Minas Gerais, Brazil (2010-2019)
Cad Saude Publica. 2024 Mar 25;40(3):e00076723. doi: 10.1590/0102-311XPT076723. eCollection 2024.ABSTRACTAir temperature is a climatic factor that affects the incidence of dengue, with effects varying according to time and space. We investigated the relationship between minimum air temperature and dengue incidence in Minas Gerais, Brazil, and evaluated the influence of socioeconomic and geographic variables on this relationship. This is a time series study with analysis conducted in three distinct stages: modeling using a distributed lag non-linear model, meta-analysis of models obtained, and meta-regression with geographi...
Source: Cadernos de Saude Publica - March 27, 2024 Category: International Medicine & Public Health Authors: Jo ão Pedro Medeiros Gomes Igor Magaton Ribas Pedro Augusto Rosa Valadares Lucas Santos Jardim M ário Círio Nogueira C ássia de Castro Martins Ferreira Aripuan ã Sakurada Aranha Watanabe Let ícia de Castro Martins Ferreira Source Type: research
Development of Multiplex Molecular Assays for Simultaneous Detection of Dengue Serotypes and Chikungunya Virus for Arbovirus Surveillance
Curr Issues Mol Biol. 2024 Mar 6;46(3):2093-2104. doi: 10.3390/cimb46030134.ABSTRACTThe major arboviruses mainly belong to the Bunyaviridae, Togaviridae, and Flaviviridae families, among which the chikungunya virus and dengue virus have emerged as global public health problems. The main objective of this study was to develop specific, sensitive, and cost-effective molecular multiplex RT-PCR and RT-qPCR assays for the rapid and simultaneous detection of CHIKV and the four serotypes of DENV for arbovirus surveillance. Specific primers for all viruses were designed, and one-step multiplex RT-PCR (mRT-PCR) and RT-qPCR (mRT-qPC...
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Louis Robert W Belem Sylvester Agha Ibemgbo Michel Kir éopori Gomgnimbou Dileep Kumar Verma Antoinette Kabor é Ankit Kumar Ibrahim Sangar é Sujatha Sunil Source Type: research
Relationship between air temperature and dengue incidence: time series study in Minas Gerais, Brazil (2010-2019)
Cad Saude Publica. 2024 Mar 25;40(3):e00076723. doi: 10.1590/0102-311XPT076723. eCollection 2024.ABSTRACTAir temperature is a climatic factor that affects the incidence of dengue, with effects varying according to time and space. We investigated the relationship between minimum air temperature and dengue incidence in Minas Gerais, Brazil, and evaluated the influence of socioeconomic and geographic variables on this relationship. This is a time series study with analysis conducted in three distinct stages: modeling using a distributed lag non-linear model, meta-analysis of models obtained, and meta-regression with geographi...
Source: Cadernos de Saude Publica - March 27, 2024 Category: International Medicine & Public Health Authors: Jo ão Pedro Medeiros Gomes Igor Magaton Ribas Pedro Augusto Rosa Valadares Lucas Santos Jardim M ário Círio Nogueira C ássia de Castro Martins Ferreira Aripuan ã Sakurada Aranha Watanabe Let ícia de Castro Martins Ferreira Source Type: research
Development of Multiplex Molecular Assays for Simultaneous Detection of Dengue Serotypes and Chikungunya Virus for Arbovirus Surveillance
Curr Issues Mol Biol. 2024 Mar 6;46(3):2093-2104. doi: 10.3390/cimb46030134.ABSTRACTThe major arboviruses mainly belong to the Bunyaviridae, Togaviridae, and Flaviviridae families, among which the chikungunya virus and dengue virus have emerged as global public health problems. The main objective of this study was to develop specific, sensitive, and cost-effective molecular multiplex RT-PCR and RT-qPCR assays for the rapid and simultaneous detection of CHIKV and the four serotypes of DENV for arbovirus surveillance. Specific primers for all viruses were designed, and one-step multiplex RT-PCR (mRT-PCR) and RT-qPCR (mRT-qPC...
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Louis Robert W Belem Sylvester Agha Ibemgbo Michel Kir éopori Gomgnimbou Dileep Kumar Verma Antoinette Kabor é Ankit Kumar Ibrahim Sangar é Sujatha Sunil Source Type: research
Relationship between air temperature and dengue incidence: time series study in Minas Gerais, Brazil (2010-2019)
Cad Saude Publica. 2024 Mar 25;40(3):e00076723. doi: 10.1590/0102-311XPT076723. eCollection 2024.ABSTRACTAir temperature is a climatic factor that affects the incidence of dengue, with effects varying according to time and space. We investigated the relationship between minimum air temperature and dengue incidence in Minas Gerais, Brazil, and evaluated the influence of socioeconomic and geographic variables on this relationship. This is a time series study with analysis conducted in three distinct stages: modeling using a distributed lag non-linear model, meta-analysis of models obtained, and meta-regression with geographi...
Source: Cadernos de Saude Publica - March 27, 2024 Category: International Medicine & Public Health Authors: Jo ão Pedro Medeiros Gomes Igor Magaton Ribas Pedro Augusto Rosa Valadares Lucas Santos Jardim M ário Círio Nogueira C ássia de Castro Martins Ferreira Aripuan ã Sakurada Aranha Watanabe Let ícia de Castro Martins Ferreira Source Type: research
Development of Multiplex Molecular Assays for Simultaneous Detection of Dengue Serotypes and Chikungunya Virus for Arbovirus Surveillance
Curr Issues Mol Biol. 2024 Mar 6;46(3):2093-2104. doi: 10.3390/cimb46030134.ABSTRACTThe major arboviruses mainly belong to the Bunyaviridae, Togaviridae, and Flaviviridae families, among which the chikungunya virus and dengue virus have emerged as global public health problems. The main objective of this study was to develop specific, sensitive, and cost-effective molecular multiplex RT-PCR and RT-qPCR assays for the rapid and simultaneous detection of CHIKV and the four serotypes of DENV for arbovirus surveillance. Specific primers for all viruses were designed, and one-step multiplex RT-PCR (mRT-PCR) and RT-qPCR (mRT-qPC...
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Louis Robert W Belem Sylvester Agha Ibemgbo Michel Kir éopori Gomgnimbou Dileep Kumar Verma Antoinette Kabor é Ankit Kumar Ibrahim Sangar é Sujatha Sunil Source Type: research
Relationship between air temperature and dengue incidence: time series study in Minas Gerais, Brazil (2010-2019)
Cad Saude Publica. 2024 Mar 25;40(3):e00076723. doi: 10.1590/0102-311XPT076723. eCollection 2024.ABSTRACTAir temperature is a climatic factor that affects the incidence of dengue, with effects varying according to time and space. We investigated the relationship between minimum air temperature and dengue incidence in Minas Gerais, Brazil, and evaluated the influence of socioeconomic and geographic variables on this relationship. This is a time series study with analysis conducted in three distinct stages: modeling using a distributed lag non-linear model, meta-analysis of models obtained, and meta-regression with geographi...
Source: Cadernos de Saude Publica - March 27, 2024 Category: International Medicine & Public Health Authors: Jo ão Pedro Medeiros Gomes Igor Magaton Ribas Pedro Augusto Rosa Valadares Lucas Santos Jardim M ário Círio Nogueira C ássia de Castro Martins Ferreira Aripuan ã Sakurada Aranha Watanabe Let ícia de Castro Martins Ferreira Source Type: research
Development of Multiplex Molecular Assays for Simultaneous Detection of Dengue Serotypes and Chikungunya Virus for Arbovirus Surveillance
Curr Issues Mol Biol. 2024 Mar 6;46(3):2093-2104. doi: 10.3390/cimb46030134.ABSTRACTThe major arboviruses mainly belong to the Bunyaviridae, Togaviridae, and Flaviviridae families, among which the chikungunya virus and dengue virus have emerged as global public health problems. The main objective of this study was to develop specific, sensitive, and cost-effective molecular multiplex RT-PCR and RT-qPCR assays for the rapid and simultaneous detection of CHIKV and the four serotypes of DENV for arbovirus surveillance. Specific primers for all viruses were designed, and one-step multiplex RT-PCR (mRT-PCR) and RT-qPCR (mRT-qPC...
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Louis Robert W Belem Sylvester Agha Ibemgbo Michel Kir éopori Gomgnimbou Dileep Kumar Verma Antoinette Kabor é Ankit Kumar Ibrahim Sangar é Sujatha Sunil Source Type: research
Relationship between air temperature and dengue incidence: time series study in Minas Gerais, Brazil (2010-2019)
Cad Saude Publica. 2024 Mar 25;40(3):e00076723. doi: 10.1590/0102-311XPT076723. eCollection 2024.ABSTRACTAir temperature is a climatic factor that affects the incidence of dengue, with effects varying according to time and space. We investigated the relationship between minimum air temperature and dengue incidence in Minas Gerais, Brazil, and evaluated the influence of socioeconomic and geographic variables on this relationship. This is a time series study with analysis conducted in three distinct stages: modeling using a distributed lag non-linear model, meta-analysis of models obtained, and meta-regression with geographi...
Source: Cadernos de Saude Publica - March 27, 2024 Category: International Medicine & Public Health Authors: Jo ão Pedro Medeiros Gomes Igor Magaton Ribas Pedro Augusto Rosa Valadares Lucas Santos Jardim M ário Círio Nogueira C ássia de Castro Martins Ferreira Aripuan ã Sakurada Aranha Watanabe Let ícia de Castro Martins Ferreira Source Type: research
Development of Multiplex Molecular Assays for Simultaneous Detection of Dengue Serotypes and Chikungunya Virus for Arbovirus Surveillance
Curr Issues Mol Biol. 2024 Mar 6;46(3):2093-2104. doi: 10.3390/cimb46030134.ABSTRACTThe major arboviruses mainly belong to the Bunyaviridae, Togaviridae, and Flaviviridae families, among which the chikungunya virus and dengue virus have emerged as global public health problems. The main objective of this study was to develop specific, sensitive, and cost-effective molecular multiplex RT-PCR and RT-qPCR assays for the rapid and simultaneous detection of CHIKV and the four serotypes of DENV for arbovirus surveillance. Specific primers for all viruses were designed, and one-step multiplex RT-PCR (mRT-PCR) and RT-qPCR (mRT-qPC...
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Louis Robert W Belem Sylvester Agha Ibemgbo Michel Kir éopori Gomgnimbou Dileep Kumar Verma Antoinette Kabor é Ankit Kumar Ibrahim Sangar é Sujatha Sunil Source Type: research
