The Burkholderia thailandensis Phages ΦE058 and ΦE067 Represent Distinct Prototypes of a New Subgroup of Temperate Burkholderia Myoviruses
In this study we isolated two phages from B. pseudomallei and three phages from B. thailandensis and determined their morphology, host range, and relationship. All five phages belong to the family Myoviridae, but some of them revealed different host specificities. DNA-DNA hybridization experiments indicated that the phages belong to two groups. One group, composed of ΦE058 (44,121 bp) and ΦE067 (43,649 bp), represents a new subgroup of Burkholderia myoviruses that is not related to known phages. The genomes of ΦE058 and ΦE067 are similar but also show some striking differences. Repressor proteins differ clearly allowin...
Source: Frontiers in Microbiology - May 26, 2020 Category: Microbiology Source Type: research
Development of a microsphere-based immunoassay for the serological detection of glanders in equids.
Abstract
Burkholderia mallei is the etiologic agent of glanders, an infectious disease of solipeds, with renewed scientific interest due to its increasing incidence in different parts of the world. More rapid, sensitive and specific assays are required by laboratories for confirmatory testing of this disease. A microsphere-based immunoassay consisting of beads coated with B. mallei recombinant proteins (BimA, GroEL, Hcp1, and TssB) has been developed for the serological diagnosis of glanders. The proteins' performance was compared with the OIE reference complement fixation test (CFT) and an indirect enzyme...
Source: Acta Tropica - April 13, 2020 Category: Infectious Diseases Authors: Laroucau K, Saqib M, Martin B, Deshayes T, Bertin C, Wernery U, Joseph S, Singha H, Tripathi BN, Beck C Tags: Acta Trop Source Type: research
Combinations of early generation antibiotics and antimicrobial peptides are effective against a broad spectrum of bacterial biothreat agents
In this study, the therapeutic potential of combinations of an antibiotic and a broad-spectrum antimicrobial peptide (AMP) was evaluated against five bacterial biothreats, the etiologic agents of glanders (Burkholderia mallei), melioidosis (Burkholderia pseudomallei), plague (Yersinia pestis), tularemia (Francisella tularensis), and anthrax (Bacillus anthracis). The therapeutics included licensed early generation antibiotics which are now rarely used. Three antibiotics and one 24- amino acid AMP were selected based on MIC assay data. Combinations of the AMP and tigecycline, minocycline, or novobiocin were screened for syne...
Source: Microbial Pathogenesis - February 9, 2020 Category: Infectious Diseases Source Type: research
Virulence from the rhizosphere: ecology and evolution of Burkholderia pseudomallei-complex species.
Abstract
Burkholderia pseudomallei (Bp) and Burkholderia mallei (Bm) cause the often-lethal infectious diseases melioidosis and glanders, respectively. Curiously, closely related species within the Bp complex share a nearly identical arsenal of virulence traits, yet are harmless to humans. Clues to the origin of virulence in this group can be found in their genetics and ecology. As a resident of the rhizosphere, Bp faces competition for nutrients and predation by other organisms. Adaptation over millennia has enabled Bp to accumulate mechanisms that overlap in their ability to promote fitness in the enviro...
Source: Current Opinion in Microbiology - February 2, 2020 Category: Microbiology Authors: French CT, Bulterys PL, Woodward CL, Tatters AO, Ng KR, Miller JF Tags: Curr Opin Microbiol Source Type: research
Molecules, Vol. 24, Pages 4516: A Machine Learning-Based Raman Spectroscopic Assay for the Identification of Burkholderia mallei and Related Species
inrich Neubauer
Burkholderia (B.) mallei, the causative agent of glanders, and B. pseudomallei, the causative agent of melioidosis in humans and animals, are genetically closely related. The high infectious potential of both organisms, their serological cross-reactivity, and similar clinical symptoms in human and animals make the differentiation from each other and other Burkholderia species challenging. The increased resistance against many antibiotics implies the need for fast and robust identification methods. The use of Raman microspectroscopy in microbial diagnostic has the potential for rapid and reliable identif...
Source: Molecules - December 9, 2019 Category: Chemistry Authors: Amira A. Moawad Anja Silge Thomas Bocklitz Katja Fischer Petra R ösch Uwe Roesler Mandy C. Elschner J ürgen Popp Heinrich Neubauer Tags: Article Source Type: research
Lipopolysaccharides from Different Burkholderia Species with Different Lipid A Structures Induce Toll-Like Receptor 4 Activation and React with Melioidosis Patient Sera [Host Response and Inflammation]
Lipopolysaccharides (LPSs) of Gram-negative bacteria comprise lipid A, core, and O-polysaccharide (OPS) components. Studies have demonstrated that LPSs isolated from the pathogenic species Burkholderia pseudomallei and Burkholderia mallei and from less-pathogenic species, such as Burkholderia thailandensis, are potent immune stimulators. The LPS structure of B. pseudomallei, the causative agent of melioidosis, is highly conserved in isolates from Thailand; however, the LPSs isolated from other, related species have not been characterized to enable understanding of their immune recognition and antigenicities. Here, we descr...
Source: Infection and Immunity - November 17, 2019 Category: Infectious Diseases Authors: Sengyee, S., Yoon, S. H., West, T. E., Ernst, R. K., Chantratita, N. Tags: Host Response and Inflammation Source Type: research
Untargeted accurate identification of highly pathogenic bacteria directly from blood culture flasks.
Abstract
To improve the preparedness against exposure to highly pathogenic bacteria and to anticipate the wide variety of bacteria that can cause bloodstream infections (BSIs), a safe, unbiased and highly accurate identification method was developed. Our liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based method can identify highly pathogenic bacteria, their near-neighbors and bacteria that are common causes of BSIs directly from positive blood culture flasks. The developed Peptide-Based Microbe Detection Engine (http://proteome2pathogen.com) relies on a two-step workflow: a genus-level search ...
Source: International Journal of Medical Microbiology - November 10, 2019 Category: Microbiology Authors: Berendsen EM, Levin E, Braakman R, Prodan A, van Leeuwen HC, Paauw A Tags: Int J Med Microbiol Source Type: research
GC-072: A Novel Therapeutic Candidate for Oral Treatment of Melioidosis and Infections Caused by Select Biothreat Pathogens.
Abstract
Burkholderia pseudomallei (B. pseudomallei), the etiological agent of melioidosis, is a Gram-negative bacterium with additional concern as a biothreat pathogen. The mortality rate from B. pseudomallei varies depending on the type of infection and extent of available health care, but in the case of septicemia left untreated it can range from 50 - 90%. Current therapy for melioidosis is biphasic, consisting of parenteral acute-phase treatment for two weeks or longer, followed by oral eradication-phase treatment lasting several months. An effective oral therapeutic for outpatient treatment of acute-p...
Source: Antimicrobial Agents and Chemotherapy - September 22, 2019 Category: Microbiology Authors: Shearer JD, Saylor ML, Butler CM, Treston AM, Heine HS, Chirakul S, Schweizer HP, Louie A, Drusano GL, Zumbrun SD, Warfield KL Tags: Antimicrob Agents Chemother Source Type: research
Development of an immunoblotting assay for serodiagnosis of Burkholderia mallei infection: the whole-cell proteome-based paradigm.
Conclusion: The whole-cell proteome-based immunoblot proved reliable and straightforward in our study. The prepared antigen was adaptable for application in immunoblotting. We assumed this improved immunoblotting system provides appropriate sensitivity and also specificity expected in serodiagnosis of glanders in endemic areas and typically in less-developed countries.
PMID: 31523407 [PubMed] (Source: Iranian Journal of Microbiology)
Source: Iranian Journal of Microbiology - September 18, 2019 Category: Microbiology Tags: Iran J Microbiol Source Type: research
An in situ high-throughput screen identifies inhibitors of intracellular Burkholderia pseudomallei with therapeutic efficacy [Microbiology]
Burkholderia pseudomallei (Bp) and Burkholderia mallei (Bm) are Tier-1 Select Agents that cause melioidosis and glanders, respectively. These are highly lethal human infections with limited therapeutic options. Intercellular spread is a hallmark of Burkholderia pathogenesis, and its prominent ties to virulence make it an attractive therapeutic target. We developed a... (Source: Proceedings of the National Academy of Sciences)
Source: Proceedings of the National Academy of Sciences - September 9, 2019 Category: Science Authors: Philip L. Bulterys, Isabelle J. Toesca, Michael H. Norris, Jeffrey P. Maloy, Sorel T. Fitz-Gibbon, Bryan France, Babak Toffig, Marco Morselli, Nawarat Somprasong, Matteo Pellegrini, Herbert P. Schweizer, Apichai Tuanyok, Robert Damoiseaux, Christopher T. Tags: Biological Sciences Source Type: research
Transcriptome analysis of human monocytic cells infected with Burkholderia species and exploration of pentraxin-3 as part of the innate immune response against the organisms
Burkholderia mallei (Bm) is a facultative intracellular bacterial pathogen causing highly-fatal glanders in solipeds and humans. The ability of Bm to thrive intracellularly is thought to be related to exploitatio... (Source: BMC Medical Genomics)
Source: BMC Medical Genomics - September 6, 2019 Category: Genetics & Stem Cells Authors: Sophie A. Aschenbroich, Eric R. Lafontaine, Maria Cecilia Lopez, Henry V. Baker and Robert J. Hogan Tags: Research article Source Type: research
A general protein O-glycosylation machinery conserved in Burkholderia species improves bacterial fitness and elicits glycan immunogenicity in humans [Microbiology]
The Burkholderia genus encompasses many Gram-negative bacteria living in the rhizosphere. Some Burkholderia species can cause life-threatening human infections, highlighting the need for clinical interventions targeting specific lipopolysaccharide proteins. Burkholderia cenocepacia O-linked protein glycosylation has been reported, but the chemical structure of the O-glycan and the machinery required for its biosynthesis are unknown and could reveal potential therapeutic targets. Here, using bioinformatics approaches, gene-knockout mutants, purified recombinant proteins, LC-MS–based analyses of O-glycans, and NMR-based st...
Source: Journal of Biological Chemistry - September 5, 2019 Category: Chemistry Authors: Yasmine Fathy Mohamed, Nichollas E. Scott, Antonio Molinaro, Carole Creuzenet, Ximena Ortega, Ganjana Lertmemongkolchai, Michael M. Tunney, Heather Green, Andrew M. Jones, David DeShazer, Bart J. Currie, Leonard J. Foster, Rebecca Ingram, Cristina De Cast Tags: Glycobiology and Extracellular Matrices Source Type: research
Melioidosis Patient Serum-Reactive Synthetic Tetrasaccharides Bearing the Predominant Epitopes of Burkholderia pseudomallei and Burkholderia mallei O-Antigens
Org. Biomol. Chem., 2019, Accepted Manuscript DOI: 10.1039/C9OB01711A, PaperMaude Cloutier, Emmanilo Delar, Kevin Muru, Seynabou Ndong, Robert R. Hoyeck, Taniya Kaewarpai, Narisara Chantratita, Mary N. Burtnick, Paul J. Brett, Charles Gauthier Melioidosis and glanders, respectively caused by the Gram-negative bacteriaBurkholderia pseudomallei (Bp) andBurkholderia mallei(Bm), are considered as urgent public health issues in developing countries and potential bioterrorism agents.... The content of this RSS Feed (c) The Royal Society of Chemistry (Source: RSC - Organic and Biomolecular Chemistry)
Source: RSC - Organic and Biomolecular Chemistry - September 4, 2019 Category: Molecular Biology Authors: Maude Cloutier Source Type: research
Evaluation of < i > Burkholderia mallei < /i > Δ < i > tonB < /i > Δ < i > hcp1 < /i > (CLH001) as a live attenuated vaccine in murine models of glanders and melioidosis
Conclusions/SignificanceOur data indicate that CLH001 serves as an effective live attenuated vaccine to prevent glanders and melioidosis. The quantity and quality of antibody responses as well as improving cell-mediated immune responses following vaccination need to be further investigated prior to advancement to preclinical studies. (Source: PLoS Neglected Tropical Diseases)
Source: PLoS Neglected Tropical Diseases - July 14, 2019 Category: Tropical Medicine Authors: Nittaya Khakhum Source Type: research
Molecular Mechanisms of AhpC in Resistance to Oxidative Stress in Burkholderia thailandensis
Burkholderia thailandensis is a model organism for human pathogens Burkholderia mallei and Burkholderia pseudomallei. The study of B. thailandensis peroxiredoxin is helpful for understanding the survival, pathogenic infection, and antibiotic resistance of its homologous species. Alkyl hydroperoxide reductase subunit C (AhpC) is an important peroxiredoxin involved in oxidative damage defense. Here, we report that BthAhpC exhibits broad specificity for peroxide substrates, including inorganic and organic peroxides and peroxynitrite. AhpC catalyzes the reduction of oxidants using the N-terminal conserved Cys57 as a peroxidati...
Source: Frontiers in Microbiology - July 1, 2019 Category: Microbiology Source Type: research
