Intramolecular Aglycon Delivery
Enables the Synthesis
of 6-Deoxy-β-d-manno-heptosides as Fragments of Burkholderia pseudomallei and Burkholderia mallei Capsular Polysaccharide
The Journal of Organic ChemistryDOI: 10.1021/jo500640n (Source: The Journal of Organic Chemistry)
Source: The Journal of Organic Chemistry - May 2, 2014 Category: Chemistry Authors: Marielle Tamigney Kenfack, Yves Blériot and Charles Gauthier Source Type: research
Characterization of an autotransporter adhesin protein shared by Burkholderia mallei and Burkholderia pseudomallei
BpaC, an autotransporter adhesion protein found in the pathogenic species Burkholderia mallei and Burkholderia pseudomallei, mediates adherence to respiratory epithelial cells and is expressed in vivo during the course of aerosol infection. (Source: Epidemiologic Perspectives and Innovations)
Source: Epidemiologic Perspectives and Innovations - April 14, 2014 Category: Epidemiology Authors: Eric R LafontaineRachel BalderFrank MichelRobert J Hogan Source Type: research
Evaluation of a Latex Agglutination Assay for the Identification of Burkholderia pseudomallei and Burkholderia mallei.
Abstract
Cases of melioidosis and glanders are rare in the United States, but the etiologic agents of each disease (Burkholderia pseudomallei and Burkholderia mallei, respectively) are classified as Tier 1 select agents because of concerns about their potential use as bioterrorism agents. A rapid, highly sensitive, and portable assay for clinical laboratories and field use is required. Our laboratory has further evaluated a latex agglutination assay for its ability to identify B. pseudomallei and B. mallei isolates. This assay uses a monoclonal antibody that specifically recognizes the capsular polysacchar...
Source: The American Journal of Tropical Medicine and Hygiene - April 7, 2014 Category: Tropical Medicine Authors: Duval BD, Elrod MG, Gee JE, Chantratita N, Tandhavanant S, Limmathurotsakul D, Hoffmaster AR Tags: Am J Trop Med Hyg Source Type: research
Gas chromatography–mass spectrometry method for rapid identification and differentiation of Burkholderia pseudomallei and Burkholderia mallei from each other, Burkholderia thailandensis and several members of the Burkholderia cepacia complex
ConclusionsThe method is fast, accurate and easy to use. The algorithm is robust against different growth conditions (medium and temperature).
Significance and Impact of the StudyThis assay may prove beneficial in a clinical diagnostic setting, where the rapid identification of B. pseudomallei is essential to effective treatment. This method could also be easily employed after a biological attack to confirm the presence of either B. pseudomallei or B. mallei. (Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - August 13, 2013 Category: Microbiology Authors: D. Li, J.K. March, T.M. Bills, B.C. Holt, C.E. Wilson, W. Lowe, H.D. Tolley, M.L. Lee, R.A. Robison Tags: Original Article Source Type: research
Gas chromatography‐mass spectrometry method for rapid identification and differentiation of Burkholderia pseudomallei and Burkholderia mallei from each other, Burkholderia thailandensis, and several members of the Burkholderia cepacia complex
ConclusionsThe method is fast, acurate, and easy to use. The algorithm is robust against different growth conditions (medium and temperature).
Significance and Impact of StudyThis assay may prove beneficial in a clinical diagnostic setting, where the rapid identification of B. pseudomallei is essential to effective treatment. This method could also be easily employed after a biological attack to confirm the presence of either B. pseudomallei or B. mallei.This article is protected by copyright. All rights reserved. (Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - July 24, 2013 Category: Microbiology Authors: Dan Li, Jordon K. March, Teri M. Bills, Brian C. Holt, Christopher E. Wilson, Woan Lowe, H. Dennis Tolley, Milton L. Lee, Richard A. Robison Tags: Original Article Source Type: research
Nitro versus Hydroxamate in Siderophores of Pathogenic Bacteria: Effect of Missing Hydroxylamine Protection in Malleobactin Biosynthesis.
Abstract
The elusive structure of malleobactin, a virulence factor of pathogens belonging to the Burkholderia mallei family, was finally unveiled by genetic and chemical analyses. The novel nitro-substituted siderophore is derived from an unusual, unprotected hydroxylamine, which undergoes spontaneous oxidation, as shown by in vitro assays and detection of analogues featuring hydroxylamino, nitroso, and azoxide groups.
PMID: 23821334 [PubMed - as supplied by publisher] (Source: Angewandte Chemie)
Source: Angewandte Chemie - July 2, 2013 Category: Chemistry Authors: Franke J, Ishida K, Ishida-Ito M, Hertweck C Tags: Angew Chem Int Ed Engl Source Type: research
Novel Burkholderia mallei Virulence Factors Linked to Specific Host-Pathogen Protein Interactions.
Abstract
Burkholderia mallei is an infectious intracellular pathogen whose virulence and resistance to antibiotics makes it a potential bioterrorism agent. Given its genetic origin as a commensal soil organism, it is equipped with an extensive and varied set of adapted mechanisms to cope with and modulate host-cell environments. One essential virulence mechanism constitutes the specialized secretion systems that are designed to penetrate host-cell membranes and insert pathogen proteins directly into the host cell's cytosol. However, the secretion systems' proteins and, in particular, their host targets are...
Source: Molecular and Cellular Proteomics : MCP - June 24, 2013 Category: Molecular Biology Authors: Memisevic V, Zavaljevski N, Pieper R, Rajagopala SV, Kwon K, Townsend K, Yu C, Yu X, Deshazer D, Reifman J, Wallqvist A Tags: Mol Cell Proteomics Source Type: research
Trimethoprim/sulfamethoxazole (co-trimoxazole) prophylaxis is effective against acute murine inhalational melioidosis and glanders
In this study, the antibiotic co-trimoxazole (trimethoprim/sulfamethoxazole) was assessed for the post-exposure prophylaxis of experimental infection in mice with B. pseudomallei and its close phylogenetic relative Burkholderia mallei, the causative agent of glanders. Co-trimoxazole was effective against an inhalational infection with B. pseudomallei or B. mallei. However, oral co-trimoxazole delivered twice daily did not eradicate infection when administered from 6h post exposure for 14 days or 21 days, since infected and antibiotic-treated mice succumbed to infection following relapse or immunosuppression. These data hig...
Source: International Journal of Antimicrobial Agents - March 20, 2013 Category: Drugs & Pharmacology Authors: Kay B. Barnes, Jackie Steward, Joanne E. Thwaite, M. Stephen Lever, Carwyn H. Davies, Stuart J. Armstrong, Thomas R. Laws, Neil Roughley, Sarah V. Harding, Timothy P. Atkins, Andrew J.H. Simpson, Helen S. Atkins Tags: Full length articles Source Type: research
Multiplex qPCR for reliable detection and differentiation of Burkholderia mallei and Burkholderia pseudomallei
Conclusions:
The new assay permits rapid detection of pathogenic Burkholderia and combines enhanced sensitivity, species differentiation, and inclusion of an internal control for both DNA extraction and PCR amplification. (Source: BMC Infectious Diseases)
Source: BMC Infectious Diseases - February 14, 2013 Category: Infectious Diseases Authors: Ingmar JanseRaditijo HamidjajaAmber HendriksBart van Rotterdam Source Type: research
