Batch Fermentation of Recombinant Intracellular Motility A Protein in for the Diagnosis of Equine Glanders
Burkholderia mallei is the causative agent of glanders, and it is a category B biothreat agent. A simple and rapid diagnostic tool is essential for control of glanders. We have previously shown the usefulness of a novel Burkholderia intracellular motility A (BimA) protein in glanders diagnosis. Efficient strategies for the production of this novel recombinant protein are important because of the requirement of high amounts of high-quality protein. We optimized the production and downstream process of recombinant (rBimA) protein from Escherichia coli host. (Source: Journal of Equine Veterinary Science)
Source: Journal of Equine Veterinary Science - December 11, 2014 Category: Veterinary Research Authors: Anil K. Singh, Saurabh Shrivastava, Subodh Kumar, Vijai Pal, Nataragan Gopalan Tags: Original Research Source Type: research
Batch fermentation of recombinant intracellular motility A (rBimA) protein in for the diagnosis of equine glanders
Burkholderia mallei is the causative agent of glanders and it is a category B biothreat agent. Simple and rapid diagnostic tool is essential for control of glanders. We have previously shown the usefulness of a novel Burkholderia intracellular motility A (BimA) protein in glanders diagnosis. Efficient strategies for the production of this novel recombinant protein are important because of the requirement of high amounts of high-quality protein. We optimized the production and downstream process of recombinant (rBimA) protein from Escherichia coli host. (Source: Journal of Equine Veterinary Science)
Source: Journal of Equine Veterinary Science - December 11, 2014 Category: Veterinary Research Authors: Anil K. Singh, Saurabh Shrivastava, Subodh Kumar, Vijai Pal, N. Gopalan Source Type: research
Oropharyngeal Aspiration of Burkholderia mallei and Burkholderia pseudomallei in BALB/c Mice
by Kevin L. Schully, Matthew G. Bell, Jerrold M. Ward, Andrea M. Keane-Myers
Burkholderia mallei and Burkholderia pseudomallei are potentially lethal pathogens categorized as biothreat agents due, in part, to their ability to be disseminated via aerosol. There are no protective vaccines against these pathogens and treatment options are limited and cumbersome. Since disease severity is greatest when these agents are inhaled, efforts to develop pre- or post-exposure prophylaxis focus largely on inhalation models of infection. Here, we demonstrate a non-invasive and technically simple method for affecting the inhalational ch...
Source: PLoS One - December 11, 2014 Category: Biomedical Science Authors: Kevin L. Schully et al. Source Type: research
Synthesis and characterization of biocompatible tenorite nanoparticles and potential property against biofilm formation
Publication date: Available online 26 November 2014 Source:Saudi Pharmaceutical Journal Author(s): Davoodbasha MubarakAli , Jegatheesan Arunkumar , Pratheesh Pooja , Gopalakrishnan Subramanian , Nooruddin Thajuddin To assess the anti-biofilm property of tenorite nanoparticles and to study their suitability as a possible coating material for medical implants. Tenorite (CuO) nanoparticles were synthesized by the optimized thermal decomposition method and characterized using TEM, XRD, FTIR and Uv-Vis analysis. Their influence on biofilm forming of microbes was studied by growing multi drug resistant bacterial strains...
Source: Saudi Pharmaceutical Journal - November 26, 2014 Category: Drugs & Pharmacology Source Type: research
Characterization of cellular immune response and innate immune signaling in human and nonhuman primate primary mononuclear cells exposed to Burkholderia mallei
In this study, we examined the primary cellular immune response to B. mallei in PBMC cultures of non-human primates (NHPs), Chlorocebus aethiops (African Green Monkeys), Macaca fascicularis (Cynomolgus macaque), and Macaca mulatta (Rhesus macaque) and humans. Our results demonstrated that B. mallei elicited strong primary pro-inflammatory cytokines (IFN-γ, TNF-α, IL-1β, and IL-6) equivalent to the levels of B. pseudomallei in primary PBMC cultures of NHPs and humans. When we examined IL-1β and other cytokine responses by comparison to Escherichia coli LPS, African Green Monkeys appears to be most responsive to B. m...
Source: Microbial Pathogenesis - November 20, 2014 Category: Infectious Diseases Source Type: research
Immunopotentiation for Bacterial Biodefense.
Abstract
Activation of the innate immune system can enhance resistance to a variety of bacterial and viral infections. In situations where the etiological agent of disease is unknown, such as a bioterror attack, stimulation of innate immunity may be particularly useful as induced immune responses are often capable of providing protection against a broad range of pathogens. In particular, the threat of an intentional release of a highly virulent bacterial pathogen that is either intrinsically resistant to antibiotics, or has been weaponized via the introduction of antibiotic resistance, makes immunopotentia...
Source: Current Topics in Medicinal Chemistry - November 6, 2014 Category: Chemistry Authors: Skyberg JA Tags: Curr Top Med Chem Source Type: research
Structure‐Based Design and Synthesis of a Small Molecule that Exhibits Anti‐inflammatory Activity by Inhibition of MyD88‐mediated Signaling to Bacterial Toxin Exposure
This article is protected by copyright. All rights reserved.
A synthetic dimeric compound 4210 mimicking BB‐loop of MyD88 was evaluated for potential toxin therapeutic in context with the inhibition of MyD88‐mediated signaling for proinflammatory responses with exposure to bacterial toxins. In a cell based reporter and biochemical assays, the compound 4210 demonstrates anti‐inflammatory activity by targeting MyD88, attenuated cytokine production in human primary cultures, and protected mice from lethal toxic Shock. (Source: Chemical Biology and Drug Design)
Source: Chemical Biology and Drug Design - November 1, 2014 Category: Biology Authors: Shahabuddin Alam, Sacha Javor, Melissa Degardin, Dariush Ajami, Mitra Rebek, Teri L. Kissner, David M. Waag, Julius Rebek, Kamal U. Saikh Tags: Research Article Source Type: research
Comparative experimental subcutaneous glanders and melioidosis in the common marmoset (Callithrix jacchus)
Summary
Glanders and melioidosis are caused by two distinct Burkholderia species and have generally been considered to have similar disease progression. While both of these pathogens are HHS/CDC Tier 1 agents, natural infection with both these pathogens is primarily through skin inoculation. The common marmoset (Callithrix jacchus) was used to compare disease following experimental subcutaneous challenge. Acute, lethal disease was observed in marmosets following challenge with between 26 and 1.2 × 108 cfu Burkholderia pseudomallei within 22–85 h. The reproducibility and progression of the disease were assessed follo...
Source: International Journal of Experimental Pathology - October 1, 2014 Category: Pathology Authors: Michelle Nelson, Francisco J. Salguero, Rachel E. Dean, Sarah A. Ngugi, Sophie J. Smither, Timothy P. Atkins, Mark S. Lever Tags: Original Article Source Type: research
Kdo hydroxylase is an inner core assembly enzyme in the Ko-containing lipopolysaccharide biosynthesis.
Abstract
The lipopolysaccharide (LPS) isolated from certain important Gram-negative pathogens including a human pathogen Yersinia pestis and opportunistic pathogens Burkholderia mallei and Burkholderia pseudomallei contains d-glycero-d-talo-oct-2-ulosonic acid (Ko), an isosteric analog of 3-deoxy-d-manno-oct-2-ulosonic acid (Kdo). Kdo 3-hydroxylase (KdoO), a Fe(2+)/α-KG/O2 dependent dioxygenase from Burkholderia ambifaria and Yersinia pestis is responsible for Ko formation with Kdo2-lipid A as a substrate, but in which stage KdoO functions during the LPS biosynthesis has not been established. Here we puri...
Source: Biochemical and Biophysical Research communications - September 6, 2014 Category: Biochemistry Authors: Chung HS, Yang EG, Hwang D, Lee JE, Guan Z, Raetz CR Tags: Biochem Biophys Res Commun Source Type: research
A gold nanoparticle-linked glycoconjugate vaccine against
Burkholderia mallei are Gram-negative bacteria, responsible for the disease glanders. B. mallei has recently been classified as a Tier 1 agent owing to the fact that this bacterial species can be weaponised for aerosol release, has a high mortality rate and demonstrates multi-drug resistance. Furthermore, there is no licensed vaccine available against this pathogen. Lipopolysaccharide (LPS) has previously been identified as playing an important role in generating host protection against Burkholderia infection. (Source: Nanomedicine : Nanotechnology, Biology, and Medicine)
Source: Nanomedicine : Nanotechnology, Biology, and Medicine - September 3, 2014 Category: Nanotechnology Authors: Anthony E. Gregory, Barbara M. Judy, Omar Qazi, Carla A. Blumentritt, Katherine A. Brown, Andrew M. Shaw, Alfredo G. Torres, Richard W. Titball Source Type: research
Inactivation of bacterial and viral biothreat agents on metallic copper surfaces.
Abstract
In recent years several studies in laboratory settings and in hospital environments have demonstrated that surfaces of massive metallic copper have intrinsic antibacterial and antiviral properties. Microbes are rapidly inactivated by a quick, sharp shock known as contact killing. The underlying mechanism is not yet fully understood; however, in this process the cytoplasmic membrane is severely damaged. Pathogenic bacterial and viral high-consequence species able to evade the host immune system are among the most serious lethal microbial challenges to human health. Here, we investigated contact-kil...
Source: Biometals - August 7, 2014 Category: Biochemistry Authors: Bleichert P, Espírito Santo C, Hanczaruk M, Meyer H, Grass G Tags: Biometals Source Type: research
DBSecSys: a database of Burkholderia mallei secretion systems
Conclusions:
DBSecSys provides a comprehensive, systematically organized resource of experimental and computational data associated with B. mallei secretion systems. It provides the unique ability to study secretion systems not only through characterization of their corresponding pathogen proteins, but also through characterization of their host-interacting partners.The database is available at https://applications.bhsai.org/dbsecsys. (Source: BMC Bioinformatics - Latest articles)
Source: BMC Bioinformatics - Latest articles - July 16, 2014 Category: Bioinformatics Authors: Vesna Memi¿evi¿Kamal KumarLi ChengNela ZavaljevskiDavid DeShazerAnders WallqvistJaques Reifman Source Type: research
Construction and characterization of stable, constitutively expressed, chromosomal green and red fluorescent transcriptional fusions in the select agents, Bacillus anthracis, Yersinia pestis, Burkholderia mallei, and Burkholderia pseudomallei
Abstract
Here, we constructed stable, chromosomal, constitutively expressed, green and red fluorescent protein (GFP and RFP) as reporters in the select agents, Bacillus anthracis, Yersinia pestis, Burkholderia mallei, and Burkholderia pseudomallei. Using bioinformatic approaches and other experimental analyses, we identified P0253 and P1 as potent promoters that drive the optimal expression of fluorescent reporters in single copy in B. anthracis and Burkholderia spp. as well as their surrogate strains, respectively. In comparison, Y. pestis and its surrogate strain need two chromosomal copies of cysZK promoter (P2cysZK) fo...
Source: MicrobiologyOpen - July 9, 2014 Category: Microbiology Authors: Shengchang Su, Hansraj Bangar, Roland Saldanha, Adin Pemberton, Bruce Aronow, Gary E. Dean, Thomas J. Lamkin, Daniel J. Hassett Tags: Original Research Source Type: research
Raman spectroscopic detection and identification of Burkholderia mallei and Burkholderia pseudomallei in feedstuff.
In this study, Raman microspectroscopy, a cultivation-independent typing technique for single bacterial cells with the potential for being a rapid point-of-care analysis system, is evaluated to identify and differentiate B. mallei and B. pseudomallei within hours. Here, not only broth-cultured microbes but also bacteria isolated out of pelleted animal feedstuff were taken into account. A database of Raman spectra allowed a calculation of classification functions, which were trained to differentiate Raman spectra of not only both pathogens but also of five further Burkholderia spp. and four species of the closely related ge...
Source: Analytical and Bioanalytical Chemistry - June 1, 2014 Category: Chemistry Authors: Stöckel S, Meisel S, Elschner M, Melzer F, Rösch P, Popp J Tags: Anal Bioanal Chem Source Type: research
