Impact of template denaturation prior to whole genome amplification on gene detection in high GC-content species, Burkholderia mallei and B. pseudomallei
In this study, we sought to determine the types and prevalence of antimicrobial resistance determinants (ARDs) in Burkholderia spp. strains using the Antimicrobial Resistance Determinant Microarray (ARDM). (Source: BMC Research Notes)
Source: BMC Research Notes - March 12, 2024 Category: Research Authors: Chris R. Taitt, Tomasz A. Leski, Jaimee R. Compton, Amy Chen, Kimberly L. Berk, Robert W. Dorsey, Shanmuga Sozhamannan, Dianne L. Dutt and Gary J. Vora Tags: Research Note Source Type: research
Foremost report of the whole genome of Spirabiliibacterium mucosae from India and comparative genomics of the novel genus Spirabiliibacterium
Gene. 2023 Mar 12:147359. doi: 10.1016/j.gene.2023.147359. Online ahead of print.ABSTRACTSeveral Pasteurella like organisms isolated from various avian species were recently reclassified into new genus based on whole genome sequence analysis. One such Pasteurella like organism, Bisgaard taxon 14 was classified as Spirabiliibacterium mucosae. In the present study, a Gram-negative organism was isolated from ailing pigeons with respiratory infection from a farm in Tamil Nadu, India and the organism was misidentified as Burkholderia mallei by Vitek 2 compact system based on biochemical characterization. Since, B. mallei is hig...
Source: Gene - March 14, 2023 Category: Genetics & Stem Cells Authors: Kumaragurubaran Karthik Subbaiyan Anbazhagan Murugesan Ananda Chitra Rajendran Ramya Ramaswamy Sridhar Gopal Dhinakar Raj Source Type: research
Foremost report of the whole genome of Spirabiliibacterium mucosae from India and comparative genomics of the novel genus Spirabiliibacterium
Gene. 2023 Mar 12:147359. doi: 10.1016/j.gene.2023.147359. Online ahead of print.ABSTRACTSeveral Pasteurella like organisms isolated from various avian species were recently reclassified into new genus based on whole genome sequence analysis. One such Pasteurella like organism, Bisgaard taxon 14 was classified as Spirabiliibacterium mucosae. In the present study, a Gram-negative organism was isolated from ailing pigeons with respiratory infection from a farm in Tamil Nadu, India and the organism was misidentified as Burkholderia mallei by Vitek 2 compact system based on biochemical characterization. Since, B. mallei is hig...
Source: Gene - March 14, 2023 Category: Genetics & Stem Cells Authors: Kumaragurubaran Karthik Subbaiyan Anbazhagan Murugesan Ananda Chitra Rajendran Ramya Ramaswamy Sridhar Gopal Dhinakar Raj Source Type: research
Foremost report of the whole genome of Spirabiliibacterium mucosae from India and comparative genomics of the novel genus Spirabiliibacterium
Gene. 2023 Mar 12:147359. doi: 10.1016/j.gene.2023.147359. Online ahead of print.ABSTRACTSeveral Pasteurella like organisms isolated from various avian species were recently reclassified into new genus based on whole genome sequence analysis. One such Pasteurella like organism, Bisgaard taxon 14 was classified as Spirabiliibacterium mucosae. In the present study, a Gram-negative organism was isolated from ailing pigeons with respiratory infection from a farm in Tamil Nadu, India and the organism was misidentified as Burkholderia mallei by Vitek 2 compact system based on biochemical characterization. Since, B. mallei is hig...
Source: Gene - March 14, 2023 Category: Genetics & Stem Cells Authors: Kumaragurubaran Karthik Subbaiyan Anbazhagan Murugesan Ananda Chitra Rajendran Ramya Ramaswamy Sridhar Gopal Dhinakar Raj Source Type: research
Expression of virulence and antimicrobial related proteins in < i > Burkholderia mallei < /i > and < i > Burkholderia pseudomallei < /i >
ConclusionsAnalyzing the expressed proteomes revealed differences betweenB.mallei andB.pseudomallei but also between isolates from the same species. Proteome analysis can be used not only to identifyB.mallei andB.pseudomallei but also to characterize the presence of important factors that putatively contribute to the pathogenesis ofB.mallei andB.pseudomallei. (Source: PLoS Neglected Tropical Diseases)
Source: PLoS Neglected Tropical Diseases - January 6, 2023 Category: Tropical Medicine Authors: Armand Paauw Source Type: research
Evaluation of two different vaccine platforms for immunization against melioidosis and glanders
Burkholderia pseudomallei and the closely related species, Burkholderia mallei, produce similar multifaceted diseases which range from rapidly fatal to protracted and chronic, and are a major cause of mortality in endemic regions. Besides causing natural infections, both microbes are Tier 1 potential biothreat agents. Antibiotic treatment is prolonged with variable results, hence effective vaccines are urgently needed. The purpose of our studies was to compare candidate vaccines that target both melioidosis and glanders to identify the most efficacious one(s) and define residual requirements for their transition to the non...
Source: Frontiers in Microbiology - August 17, 2022 Category: Microbiology Source Type: research
A case report of Burkholderia mallei infection leading to pneumonia
CONCLUSIONS: Glanders is a rare disease that can cause skin infection, lymphadenitis, and pneumonia and in severe cases, it can be life-threatening. The diagnosis of this disease mainly relies on microbiological culture and pathological biopsy. Diagnosis is also facilitated by multiplex PCR-based targeted gene sequencing facilitates the diagnosis of this disease. Glanders is treated with cephalosporins, carbapenems, and other sensitive antibiotics.PMID:35579163 | DOI:10.2174/1386207325666220509152221 (Source: Combinatorial Chemistry and High Throughput Screening)
Source: Combinatorial Chemistry and High Throughput Screening - May 17, 2022 Category: Chemistry Authors: Guanfeng He Yu Zeng Qizhong He Tuxuan Liu Nanhong Li Hui Lin Muhong Zeng Yonglong Li Min Peng Junfen Cheng Wang Liu Weimin Yao Source Type: research
A case report of Burkholderia mallei infection leading to pneumonia
CONCLUSIONS: Glanders is a rare disease that can cause skin infection, lymphadenitis, and pneumonia and in severe cases, it can be life-threatening. The diagnosis of this disease mainly relies on microbiological culture and pathological biopsy. Diagnosis is also facilitated by multiplex PCR-based targeted gene sequencing facilitates the diagnosis of this disease. Glanders is treated with cephalosporins, carbapenems, and other sensitive antibiotics.PMID:35579163 | DOI:10.2174/1386207325666220509152221 (Source: Combinatorial Chemistry and High Throughput Screening)
Source: Combinatorial Chemistry and High Throughput Screening - May 17, 2022 Category: Chemistry Authors: Guanfeng He Yu Zeng Qizhong He Tuxuan Liu Nanhong Li Hui Lin Muhong Zeng Yonglong Li Min Peng Junfen Cheng Wang Liu Weimin Yao Source Type: research
A case report of Burkholderia mallei infection leading to pneumonia
CONCLUSIONS: Glanders is a rare disease that can cause skin infection, lymphadenitis, and pneumonia and in severe cases, it can be life-threatening. The diagnosis of this disease mainly relies on microbiological culture and pathological biopsy. Diagnosis is also facilitated by multiplex PCR-based targeted gene sequencing facilitates the diagnosis of this disease. Glanders is treated with cephalosporins, carbapenems, and other sensitive antibiotics.PMID:35579163 | DOI:10.2174/1386207325666220509152221 (Source: Combinatorial Chemistry and High Throughput Screening)
Source: Combinatorial Chemistry and High Throughput Screening - May 17, 2022 Category: Chemistry Authors: Guanfeng He Yu Zeng Qizhong He Tuxuan Liu Nanhong Li Hui Lin Muhong Zeng Yonglong Li Min Peng Junfen Cheng Wang Liu Weimin Yao Source Type: research
First glanders cases detected in Nepal underscore the need for surveillance and border controls
Glanders is a transmissible zoonotic disease caused by Burkholderia mallei that infects equids and humans. No glanders cases in equids were reported so far in Nepal. (Source: BMC Veterinary Research)
Source: BMC Veterinary Research - April 6, 2022 Category: Veterinary Research Authors: Koirala P, Maharjan M, Manandhar S, Pandey KR, Deshayes T, Wang G, Valvano MA and Laroucau K Tags: Case Report Source Type: research
Molecular characterization of Burkholderia mallei strains isolated from horses in Brazil (2014-2017)
The objective of this work was to provide additional knowledge on the diversity of the strains circulating in Brazil. Six Burkholderia mallei isolates obtained during necropsies of glanderous horses between 2014 and 2017 in two different states (Pernambuco and Alagoas) were analyzed by polymerase chain reaction-high-resolution melting (PCR-HRM). While four strains (9902 RSC, BM_campo 1, BM_campo 3 and UFAL2) clustered in the L3B2 branch, which already includes the Brazilian 16-2438_BM#8 strain, two strains (BM_campo 2.1 and BM_campo 2.2) clustered within the L3B3sB3 branch, which mostly includes older isolates, from Europe...
Source: Infection, Genetics and Evolution - February 20, 2022 Category: Genetics & Stem Cells Authors: Marcus V D Falc ão Karine Laroucau Fabien Vorimore Thomas Deshayes Vania L A Santana Karla P C Silva Sergio A do Nascimento Roberto S de Castro Flabio R Ara újo Rinaldo A Mota Source Type: research
Molecular characterization of Burkholderia mallei strains isolated from horses in Brazil (2014-2017)
The objective of this work was to provide additional knowledge on the diversity of the strains circulating in Brazil. Six Burkholderia mallei isolates obtained during necropsies of glanderous horses between 2014 and 2017 in two different states (Pernambuco and Alagoas) were analyzed by polymerase chain reaction-high-resolution melting (PCR-HRM). While four strains (9902 RSC, BM_campo 1, BM_campo 3 and UFAL2) clustered in the L3B2 branch, which already includes the Brazilian 16-2438_BM#8 strain, two strains (BM_campo 2.1 and BM_campo 2.2) clustered within the L3B3sB3 branch, which mostly includes older isolates, from Europe...
Source: Infection, Genetics and Evolution - February 20, 2022 Category: Genetics & Stem Cells Authors: Marcus V D Falc ão Karine Laroucau Fabien Vorimore Thomas Deshayes Vania L A Santana Karla P C Silva Sergio A do Nascimento Roberto S de Castro Flabio R Ara újo Rinaldo A Mota Source Type: research
Polymerase Spiral Reaction (PSR) as a novel rapid colorimetric isothermal point of care assay for detection of Trypanosoma evansi genomic DNA
Vet Parasitol. 2021 Dec 28;302:109644. doi: 10.1016/j.vetpar.2021.109644. Online ahead of print.ABSTRACTPolymerase spiral reaction (PSR) opens new avenues for specific diagnosis of pathogens known for cryptic infection at field level and its application is still unexplored in the field of parasitology. The present study aimed to explore and optimize colorimetric based PSR technique for the detection of Trypanosoma evansi in the blood of the host by targeting the 196bp Invariable Surface Glycoprotein (ISG) gene of Trypanosoma evansi. The specificity of the test was determined against Theileria equi, Theileria annulata, Babe...
Source: Herpes - January 15, 2022 Category: Infectious Diseases Authors: Diksha Sharma Snehil Gupta Khushboo Sethi Sanjay Kumar Rajender Kumar Source Type: research
Development of a sensitive competitive enzyme-linked immunosorbent assay for serodiagnosis of < i > Burkholderia mallei < /i > , a Tier 1 select agent
In this study, we raised a monoclonal antibody (mAb) against the lipopolysaccharide (LPS) ofB.mallei and developed a competitive enzyme-linked immunosorbent assay (cELISA) forB.mallei infection. Using the titrated optimal conditions ofB.mallei-LPS (2 ng) for microtiter plate coating, sample serum dilution at 1:20 and 3.5 ng/ μL anti-LPS mAb B5, the cutoff value of the cELISA was determined using serum samples from 136 glanders-free seronegative horses in Hong Kong. All calculated percentage inhibition (PI) values from these seronegative samples were below 39.6% inhibition (1.5 standard deviations above mean PI) and was us...
Source: PLoS Neglected Tropical Diseases - December 21, 2021 Category: Tropical Medicine Authors: Ulrich Wernery Source Type: research
16S rDNA and ITS Sequence Diversity of Burkholderia mallei Isolated from Glanders-Affected Horses and Mules in India (2013-2019)
This study implies that 16S rDNA and ITS region may be used for molecular characterization of B. mallei associated with glanders in resource-limited settings.PMID:34921617 | DOI:10.1007/s00284-021-02701-8 (Source: Current Microbiology)
Source: Current Microbiology - December 18, 2021 Category: Microbiology Authors: Karuppusamy Shanmugasundaram Harisankar Singha Sheetal Saini Bhupendra N Tripathi Source Type: research
