Unraveling DEHP influence on hemoglobin S polymerization in sickle cell disease: Ex vivo, in vitro and in silico analysis
This study aimed to unravel DEHP effect on SCD patient's hemoglobin function. We found that HbS polymerization using whole erythrocytes is decreased by DEHP in ex vivo experiments and this effect might be mediated by the DEHP-VAL6 interaction, evaluated in silico. Isolated HbS exhibited less polymerization at low DEHP concentrations and increased polymerization rate at higher concentration. When analyzing the propensity to aggregate, HbS is more inclined to aggregate when compared to HbA due to the residue 6 mutation. Circular dichroism showed characteristic hemoglobin peaks for oxygenated HbS that are lost when oxygen is ...
Source: Toxicology in Vitro - April 23, 2024 Category: Toxicology Authors: Rodrigo Abreu Camacho Aghata Vitoria Machado Fernanda de Oliveira Mendon ça Lyzes Rosa Teixeira-Alves Camila Cristina Guimar ães-Nobre Evelyn Mendon ça-Reis Priscila Ferreira da Silva Thyago R Cardim-Pires Leandro Miranda-Alves Clemilson Berto-Junior Source Type: research
Absence of evidence of transfusion transmission risk of Creutzfeldt-Jakob disease in the United States: Results froma 28-year lookback study
We report on a 28-year lookback study of recipients of blood from donors who subsequently developed CJD.METHODS: Patients with diagnosed CJD and a history of blood donation were identified. Blood centers were asked to provide information about the distribution of the donations and consignees were requested to provide information about the recipients of the donations. Vital status of each available recipient was determined and, if deceased, the reported cause(s) of death were obtained primarily from the National Death Index. All recipients included in the study database contributed person-time up to the last recorded review...
Source: Transfusion - April 23, 2024 Category: Hematology Authors: Lauren A Crowder Roger Y Dodd Lawrence B Schonberger Source Type: research
Absence of evidence of transfusion transmission risk of Creutzfeldt-Jakob disease in the United States: Results froma 28-year lookback study
We report on a 28-year lookback study of recipients of blood from donors who subsequently developed CJD.METHODS: Patients with diagnosed CJD and a history of blood donation were identified. Blood centers were asked to provide information about the distribution of the donations and consignees were requested to provide information about the recipients of the donations. Vital status of each available recipient was determined and, if deceased, the reported cause(s) of death were obtained primarily from the National Death Index. All recipients included in the study database contributed person-time up to the last recorded review...
Source: Transfusion - April 23, 2024 Category: Hematology Authors: Lauren A Crowder Roger Y Dodd Lawrence B Schonberger Source Type: research
Unraveling DEHP influence on hemoglobin S polymerization in sickle cell disease: Ex vivo, in vitro and in silico analysis
This study aimed to unravel DEHP effect on SCD patient's hemoglobin function. We found that HbS polymerization using whole erythrocytes is decreased by DEHP in ex vivo experiments and this effect might be mediated by the DEHP-VAL6 interaction, evaluated in silico. Isolated HbS exhibited less polymerization at low DEHP concentrations and increased polymerization rate at higher concentration. When analyzing the propensity to aggregate, HbS is more inclined to aggregate when compared to HbA due to the residue 6 mutation. Circular dichroism showed characteristic hemoglobin peaks for oxygenated HbS that are lost when oxygen is ...
Source: Toxicology in Vitro - April 23, 2024 Category: Toxicology Authors: Rodrigo Abreu Camacho Aghata Vitoria Machado Fernanda de Oliveira Mendon ça Lyzes Rosa Teixeira-Alves Camila Cristina Guimar ães-Nobre Evelyn Mendon ça-Reis Priscila Ferreira da Silva Thyago R Cardim-Pires Leandro Miranda-Alves Clemilson Berto-Junior Source Type: research
Laparoscopic totally extraperitoneal hernia repair in patients with a history of previous abdominopelvic surgery
AbstractA retrospective cohort study of patients undergoing laparoscopic inguinal hernia repair compared short- and long-term outcomes between individuals with or without history of previous abdominopelvic surgery, aiming to determine the feasibility of totally extraperitoneal (TEP) repair within this population. All patients who underwent elective TEP inguinal hernia repair by one consultant surgeon across three London hospitals from January 2017 to May 2023 were retrospectively analysed to assess perioperative outcomes. Two hundred sixty-two patients were identified, of whom two hundred forty-three (93%) underwent laparo...
Source: Updates in Surgery - April 23, 2024 Category: Surgery Source Type: research
Adult Langerhans Cell Histiocytosis Presenting With Skin Lesions, Sclerosing Cholangitis and Pneumothorax
(Source: Indian Journal of Hematology and Blood Transfusion)
Source: Indian Journal of Hematology and Blood Transfusion - April 23, 2024 Category: Hematology Source Type: research
A Retrospective Analysis of BCR-ABL-1 Kinase Domain Mutations in Frontline TKI Resistant Chronic Myeloid Leukemia Patients: A Single Centre Experience
AbstractCML is a commonly diagnosed myeloproliferative neoplasm in India. Tyrosine kinase inhibitors (TKIs) are the current standard of care for management of CML. Mutations in Tyrosine kinase Domain (TKD) result in resistance to TKIs. The aim of this study is to evaluate the pattern of TKD mutations in CML patients having inadeq uate response or resistance to first line TKIs and to analyse the outcome of CML patients with and without mutations. It is a retrospective observational study. Medical records of 1633 CML patients from year 2014 to 2021 were analysed. Out of these 108 patients (6.6%) lost their response or d...
Source: Indian Journal of Hematology and Blood Transfusion - April 23, 2024 Category: Hematology Source Type: research
Robotic-Assisted Surgery for Rectal Cancer: An Expedited Summary of the Clinical Evidence
CONCLUSIONS: Robotic-assisted rectal cancer surgery may result in similar or improved clinical outcomes compared with laparoscopic and open rectal cancer surgery.PMID:38645608 | PMC:PMC11031254 (Source: Ontario Health Technology Assessment Series)
Source: Ontario Health Technology Assessment Series - April 22, 2024 Category: General Medicine Authors: Ontario Health Source Type: research
