Genome comparison of long-circulating field CnmeGV isolates from the same region
Virus Res. 2024 May 4:199390. doi: 10.1016/j.virusres.2024.199390. Online ahead of print.ABSTRACTCnaphalocrocis medinalis granulovirus (CnmeGV), belonging to Betabaculovirus cnamedinalis, can infect the rice pest, the rice leaf roller. In 1979, a CnmeGV isolate, CnmeGV-EP, was collected from Enping County, China. In 2014, we collected another CnmeGV isolate, CnmeGV-EPDH3, at the same location and obtained the complete virus genome sequence using Illumina and ONT sequencing technologies. By combining these two virus isolates, we updated the genome annotation of CnmeGV and conducted an in-depth analysis of its genome feature...
Source: Virus Research - May 6, 2024 Category: Virology Authors: Yachao Zuo Jiawen Yang Hao Zhang Lu Li Jing Luo Yanrong Lv Meijin Yuan Kai Yang Source Type: research
NLRP3-dependent pyroptosis exacerbates Coxsackievirus A16 and Coxsackievirus A10-induced inflammatory response and viral replication in SH-SY5Y cells
Virus Res. 2024 May 3:199386. doi: 10.1016/j.virusres.2024.199386. Online ahead of print.ABSTRACTCoxsackievirus A16 (CV-A16) and Coxsackievirus A10 (CV-A10), more commonly etiological agents of hand, foot and mouth disease (HFMD), are capable of causing severe neurological syndromes with high fatalities, but their neuropathogenesis has rarely been studied. Mounting evidence indicated that pyroptosis is an inflammatory form of cell death that might be widely involved in the pathogenic mechanisms of neurotropic viruses. Our study was designed to examine the effects of NLRP3-mediated pyroptosis in CV-A16- and CV-A10-induced i...
Source: Virus Research - May 5, 2024 Category: Virology Authors: Yajie Hu Wei Zhao Yaming Lv Hui Li Jiang Li Mingmei Zhong Dandan Pu Fuping Jian Jie Song Yunhui Zhang Source Type: research
NLRP3-dependent pyroptosis exacerbates Coxsackievirus A16 and Coxsackievirus A10-induced inflammatory response and viral replication in SH-SY5Y cells
Virus Res. 2024 May 3:199386. doi: 10.1016/j.virusres.2024.199386. Online ahead of print.ABSTRACTCoxsackievirus A16 (CV-A16) and Coxsackievirus A10 (CV-A10), more commonly etiological agents of hand, foot and mouth disease (HFMD), are capable of causing severe neurological syndromes with high fatalities, but their neuropathogenesis has rarely been studied. Mounting evidence indicated that pyroptosis is an inflammatory form of cell death that might be widely involved in the pathogenic mechanisms of neurotropic viruses. Our study was designed to examine the effects of NLRP3-mediated pyroptosis in CV-A16- and CV-A10-induced i...
Source: Virus Research - May 5, 2024 Category: Virology Authors: Yajie Hu Wei Zhao Yaming Lv Hui Li Jiang Li Mingmei Zhong Dandan Pu Fuping Jian Jie Song Yunhui Zhang Source Type: research
Impact of Annexin A2 on virus life cycles
Virus Res. 2024 May 1:199384. doi: 10.1016/j.virusres.2024.199384. Online ahead of print.ABSTRACTDue to the limited size of viral genomes, hijacking host machinery by the viruses taking place throughout the virus life cycle is inevitable for the survival and proliferation of the virus in the infected hosts. Recent reports indicated that Annexin A2 (AnxA2), a calcium- and lipid-binding cellular protein, plays an important role as a critical regulator in various steps of the virus life cycle. The multifarious AnxA2 functions in cells, such as adhesion, adsorption, endocytosis, exocytosis, cell proliferation and division, inf...
Source: Virus Research - May 3, 2024 Category: Virology Authors: In-Woo Park Hope K Fiadjoe Pankaj Chaudhary Source Type: research
Impact of Annexin A2 on virus life cycles
Virus Res. 2024 May 1:199384. doi: 10.1016/j.virusres.2024.199384. Online ahead of print.ABSTRACTDue to the limited size of viral genomes, hijacking host machinery by the viruses taking place throughout the virus life cycle is inevitable for the survival and proliferation of the virus in the infected hosts. Recent reports indicated that Annexin A2 (AnxA2), a calcium- and lipid-binding cellular protein, plays an important role as a critical regulator in various steps of the virus life cycle. The multifarious AnxA2 functions in cells, such as adhesion, adsorption, endocytosis, exocytosis, cell proliferation and division, inf...
Source: Virus Research - May 3, 2024 Category: Virology Authors: In-Woo Park Hope K Fiadjoe Pankaj Chaudhary Source Type: research
The alteration of NK cells phenotypes related to the functions and dengue disease outcomes
This study explored the profile of NK cells in dengue infected pediatric patients with different degrees of disease severity. DHF patients contained higher frequency of activated NK cells but lower ratio of CD56dim:CD56bright NK subsets. Activated NK cells exhibited alterations in several NK receptors. Interestingly, the frequencies of NKp30 expressing activated NK cells were more pronounced in dengue fever (DF) than in DHF pediatric patients. In vitro functional analysis indicated that degranulation of NK cells in responding to dengue infected dendritic cells (DCs) required cell-cell contact and type I IFNs. Meanwhile, In...
Source: Virus Research - May 2, 2024 Category: Virology Authors: Napas Taechasan Iris Scherwitzl Piyada Supasa Wanwisa Dejnirattisai Kanokwan Sriruksa Wannee Limpitikul Prida Malasit Gavin R Screaton Juthathip Mongkolsapaya Thaneeya Duangchinda Source Type: research
A dual-targeting approach using a human bispecific antibody against the receptor-binding domain of the Middle East Respiratory Syndrome Coronavirus
Virus Res. 2024 Apr 30:199383. doi: 10.1016/j.virusres.2024.199383. Online ahead of print.ABSTRACTThe emergence of the Middle East Respiratory Syndrome Coronavirus (MERS-CoV) has posed a significant global health concern due to its severe respiratory illness and high fatality rate. Currently, despite the potential for resurgence, there are no specific treatments for MERS-CoV, and only supportive care is available. Our study aimed to address this therapeutic gap by developing a potent neutralizing bispecific antibody (bsAb) against MERS-CoV. Initially, we isolated four human monoclonal antibodies (mAbs) that specifically ta...
Source: Virus Research - May 2, 2024 Category: Virology Authors: Ji Hyun Lee Ji Woong Kim Hee Eon Lee Jin Young Song Ah Hyun Cho Jae Hyeon Hwang Kyun Heo Sukmook Lee Source Type: research
The alteration of NK cells phenotypes related to the functions and dengue disease outcomes
This study explored the profile of NK cells in dengue infected pediatric patients with different degrees of disease severity. DHF patients contained higher frequency of activated NK cells but lower ratio of CD56dim:CD56bright NK subsets. Activated NK cells exhibited alterations in several NK receptors. Interestingly, the frequencies of NKp30 expressing activated NK cells were more pronounced in dengue fever (DF) than in DHF pediatric patients. In vitro functional analysis indicated that degranulation of NK cells in responding to dengue infected dendritic cells (DCs) required cell-cell contact and type I IFNs. Meanwhile, In...
Source: Virus Research - May 2, 2024 Category: Virology Authors: Napas Taechasan Iris Scherwitzl Piyada Supasa Wanwisa Dejnirattisai Kanokwan Sriruksa Wannee Limpitikul Prida Malasit Gavin R Screaton Juthathip Mongkolsapaya Thaneeya Duangchinda Source Type: research
A dual-targeting approach using a human bispecific antibody against the receptor-binding domain of the Middle East Respiratory Syndrome Coronavirus
Virus Res. 2024 Apr 30:199383. doi: 10.1016/j.virusres.2024.199383. Online ahead of print.ABSTRACTThe emergence of the Middle East Respiratory Syndrome Coronavirus (MERS-CoV) has posed a significant global health concern due to its severe respiratory illness and high fatality rate. Currently, despite the potential for resurgence, there are no specific treatments for MERS-CoV, and only supportive care is available. Our study aimed to address this therapeutic gap by developing a potent neutralizing bispecific antibody (bsAb) against MERS-CoV. Initially, we isolated four human monoclonal antibodies (mAbs) that specifically ta...
Source: Virus Research - May 2, 2024 Category: Virology Authors: Ji Hyun Lee Ji Woong Kim Hee Eon Lee Jin Young Song Ah Hyun Cho Jae Hyeon Hwang Kyun Heo Sukmook Lee Source Type: research
The Glycosylation Sites in RBD of Spike Protein Attenuate the Immunogenicity of PEDV AH2012/12
In this study, we constructed three recombinant PEDVs which removed the glycosylation sites in RBD. Viral infection assays revealed that similar replication characteristics between the recombinant viruses and parental PEDV. Although animal challenging study demonstrated that the glycosylation sites in RBD do not affect the pathogenicity of PEDV. However, we found that the removing the glycosylation sites on the RBD regions could promote the IgG and neutralization titer in vivo, suggesting deglycosylation in RBD could enhance the immunogenicity of PEDV. These findings demonstrated that removal of the glycosylation sites in ...
Source: Virus Research - April 28, 2024 Category: Virology Authors: Gege Zhang Qi Peng Shiyu Liu Baochao Fan Chuanhong Wang Xu Song Qiuxia Cao Chengcheng Li Hong Xu Hongting Lu Meiying Bao Shanshan Yang Yunchuan Li Jiaxiang Wang Bin Li Source Type: research
The Glycosylation Sites in RBD of Spike Protein Attenuate the Immunogenicity of PEDV AH2012/12
In this study, we constructed three recombinant PEDVs which removed the glycosylation sites in RBD. Viral infection assays revealed that similar replication characteristics between the recombinant viruses and parental PEDV. Although animal challenging study demonstrated that the glycosylation sites in RBD do not affect the pathogenicity of PEDV. However, we found that the removing the glycosylation sites on the RBD regions could promote the IgG and neutralization titer in vivo, suggesting deglycosylation in RBD could enhance the immunogenicity of PEDV. These findings demonstrated that removal of the glycosylation sites in ...
Source: Virus Research - April 28, 2024 Category: Virology Authors: Gege Zhang Qi Peng Shiyu Liu Baochao Fan Chuanhong Wang Xu Song Qiuxia Cao Chengcheng Li Hong Xu Hongting Lu Meiying Bao Shanshan Yang Yunchuan Li Jiaxiang Wang Bin Li Source Type: research
The Glycosylation Sites in RBD of Spike Protein Attenuate the Immunogenicity of PEDV AH2012/12
In this study, we constructed three recombinant PEDVs which removed the glycosylation sites in RBD. Viral infection assays revealed that similar replication characteristics between the recombinant viruses and parental PEDV. Although animal challenging study demonstrated that the glycosylation sites in RBD do not affect the pathogenicity of PEDV. However, we found that the removing the glycosylation sites on the RBD regions could promote the IgG and neutralization titer in vivo, suggesting deglycosylation in RBD could enhance the immunogenicity of PEDV. These findings demonstrated that removal of the glycosylation sites in ...
Source: Virus Research - April 28, 2024 Category: Virology Authors: Gege Zhang Qi Peng Shiyu Liu Baochao Fan Chuanhong Wang Xu Song Qiuxia Cao Chengcheng Li Hong Xu Hongting Lu Meiying Bao Shanshan Yang Yunchuan Li Jiaxiang Wang Bin Li Source Type: research
The Glycosylation Sites in RBD of Spike Protein Attenuate the Immunogenicity of PEDV AH2012/12
In this study, we constructed three recombinant PEDVs which removed the glycosylation sites in RBD. Viral infection assays revealed that similar replication characteristics between the recombinant viruses and parental PEDV. Although animal challenging study demonstrated that the glycosylation sites in RBD do not affect the pathogenicity of PEDV. However, we found that the removing the glycosylation sites on the RBD regions could promote the IgG and neutralization titer in vivo, suggesting deglycosylation in RBD could enhance the immunogenicity of PEDV. These findings demonstrated that removal of the glycosylation sites in ...
Source: Virus Research - April 28, 2024 Category: Virology Authors: Gege Zhang Qi Peng Shiyu Liu Baochao Fan Chuanhong Wang Xu Song Qiuxia Cao Chengcheng Li Hong Xu Hongting Lu Meiying Bao Shanshan Yang Yunchuan Li Jiaxiang Wang Bin Li Source Type: research
In vitro and in vivo antiviral effects of CLEVir-X against porcine reproductive and respiratory syndrome virus
Virus Res. 2024 Apr 27;345:199380. doi: 10.1016/j.virusres.2024.199380. Online ahead of print.ABSTRACTThe aim of this study was to investigate the in vitro and in vivo antiviral effects of CLEVir-X, against porcine reproductive and respiratory syndrome virus (PRRSV). CLEVir-X is a nucleoside analogue and a dialdehyde form of xanthosine. CLEVir-X demonstrated antiviral action during the in vitro portion of this experiment with its inosine monophosphate dehydrogenase (IMPDH) inhibition against PRRSV. The anti-PRRSV effect of CLEVir-X was recovered through supplementation with guanosine. This suggests that PRRSV replication m...
Source: Virus Research - April 24, 2024 Category: Virology Authors: Jeongmin Suh Sehyeong Ham Youngnam Kim Sunghun Kim Ahreum Cho Hojin Moon Chanhee Chae Source Type: research
In vitro and in vivo antiviral effects of CLEVir-X against porcine reproductive and respiratory syndrome virus
Virus Res. 2024 Apr 22:199380. doi: 10.1016/j.virusres.2024.199380. Online ahead of print.ABSTRACTThe aim of this study was to investigate the in vitro and in vivo antiviral effects of CLEVir-X, against porcine reproductive and respiratory syndrome virus (PRRSV). CLEVir-X is a nucleoside analogue and a dialdehyde form of xanthosine. CLEVir-X demonstrated antiviral action during the in vitro portion of this experiment with its inosine monophosphate dehydrogenase (IMPDH) inhibition against PRRSV. The anti-PRRSV effect of CLEVir-X was recovered through supplementation with guanosine. This suggests that PRRSV replication may b...
Source: Virus Research - April 24, 2024 Category: Virology Authors: Jeongmin Suh Sehyeong Ham Youngnam Kim Sunghun Kim Ahreum Cho Hojin Moon Chanhee Chae Source Type: research
