The Glycosylation Sites in RBD of Spike Protein Attenuate the Immunogenicity of PEDV AH2012/12

In this study, we constructed three recombinant PEDVs which removed the glycosylation sites in RBD. Viral infection assays revealed that similar replication characteristics between the recombinant viruses and parental PEDV. Although animal challenging study demonstrated that the glycosylation sites in RBD do not affect the pathogenicity of PEDV. However, we found that the removing the glycosylation sites on the RBD regions could promote the IgG and neutralization titer in vivo, suggesting deglycosylation in RBD could enhance the immunogenicity of PEDV. These findings demonstrated that removal of the glycosylation sites in RBD is a promising method to develop PEDV vaccines.PMID:38679392 | DOI:10.1016/j.virusres.2024.199381
Source: Virus Research - Category: Virology Authors: Source Type: research