Substitution of both histidines in the active site of yeast alcohol dehydrogenase 1 exposes underlying pH dependencies
Chem Biol Interact. 2024 Apr 3:110992. doi: 10.1016/j.cbi.2024.110992. Online ahead of print.ABSTRACTHistidine residues 44 and 48 in yeast alcohol dehydrogenase (ADH) bind to the coenzymes NAD(H) and contribute to catalysis. The individual H44R and H48Q substitutions alter the kinetics and pH dependencies, and now the roles of other ionizable groups in the enzyme were studied in the doubly substituted H44R/H48Q ADH. The substitutions make the enzyme more resistant to inactivation by diethyl pyrocarbonate, modestly improve affinity for coenzymes, and substantially decrease catalytic efficiencies for ethanol oxidation and ac...
Source: Chemico-Biological Interactions - April 5, 2024 Category: Molecular Biology Authors: Tobias Jacobi Darla Ann Kratzer Bryce V Plapp Source Type: research
Use of complementary medicine and its association with SARS-CoV-2 vaccination during the COVID-19 pandemic: a longitudinal cohort study
CONCLUSION: While complementary medicine is increasingly used, it is rarely discussed with primary care physicians. Complementary medicine use, especially for COVID-19 prevention, is associated with COVID-19 vaccination status. Communication between physicians, patients, and complementary medicine therapists is encouraged to facilitate a truly holistic approach to making a shared decision based on the best available information.PMID:38579302 | DOI:10.57187/s.3505 (Source: Swiss Medical Weekly)
Source: Swiss Medical Weekly - April 5, 2024 Category: General Medicine Authors: Mayssam Nehme Olivia Braillard Pierre-Yves Rodondi Idris Guessous Source Type: research
Integrated assessment of potentially toxic elements in soil of the Kangdian metallogenic province: A two-point machine learning approach
This study explored a comprehensive approach that combinescombines the spatial prediction by the two-point machine learning method and ecological-health risk assessment to quantitatively assess the comprehensive potential ecological risk index (PERI), the total hazard index (THI) and the total carcinogenic risk (TCR). The proportions of copper (Cu), cadmium (Cd), manganese (Mn), lead (Pb), zinc (Zn), and arsenic (As) concentrations exceeding the risk screening values (RSVs) were 15.03%, 5.1%, 3.72%, 1.24%, 1.1%, and 0.13%, respectively, across the 725 collected samples. Spatial prediction revealed elevated levels of As, Cd...
Source: Ecotoxicology and Environmental Safety - April 5, 2024 Category: Environmental Health Authors: Wantao Yang Liankai Zhang Bingbo Gao Xiaojie Liu Xingwu Duan Chenyi Wang Ya Zhang Qiang Li Lingqing Wang Source Type: research
Transition Metal-Free Catalytic C-H Zincation and Alumination
Angew Chem Int Ed Engl. 2024 Apr 5:e202404848. doi: 10.1002/anie.202404848. Online ahead of print.ABSTRACTC-H metalation is the most efficient method to prepare aryl-zinc and -aluminium complexes that are ubiquitous nucleophiles. Virtually all C-H metalation routes to form Al/Zn organometallics require stoichiometric, strong Brønsted bases with no base-catalyzed reactions reported. Herein we present a catalytic in amine/ammonium salt (Et3N/[(Et3N)H]+) C-H metalation process to form aryl-zinc and aryl-aluminium complexes. Key to this approach is coupling an endergonic C-H metalation step with a sufficiently exergonic dehyd...
Source: Angewandte Chemie - April 5, 2024 Category: Chemistry Authors: Milan Kumar Bisai Justyna Losiewicz Lia Sotorrios Gary Nichol Andrew Dominey Michael Cowley Stephen Thomas Stuart Macgregor Michael Ingleson Source Type: research
Zinc-ion Anchor Induced Highly Reversible Zn Anodes for High Performance Zn-ion Batteries
Angew Chem Int Ed Engl. 2024 Apr 5:e202403050. doi: 10.1002/anie.202403050. Online ahead of print.ABSTRACTUnstable Zn interface with serious detrimental parasitic side-reactions and uncontrollable Zn dendrites severely plagues the practical application of aqueous zinc-ion batteries. The interface stability was closely related to the electrolyte configuration and Zn2+ depositional behavior. In this work, a unique Zn-ion anchoring strategy is originally proposed to manipulate the coordination structure of solvated Zn-ions and guide the Zn-ion depositional behavior. Specifically, the amphoteric charged ion additives (denoted ...
Source: Angewandte Chemie - April 5, 2024 Category: Chemistry Authors: Shuang Zhou Xinyu Meng Yining Chen Jianwen Li Shangyong Lin Chao Han Xiaobo Ji Zhi Chang Anqiang Pan Source Type: research
