The measurement of vitamin D metabolites: part I-metabolism of vitamin D and the measurement of 25-hydroxyvitamin D.
Authors: Makris K, Sempos C, Cavalier E
Abstract
It has been more than 80 years since the discovery of vitamin D and its ability to cure rickets in children. Vitamin D is a secosteroid and comes in two distinct forms, vitamin D2 and vitamin D3. During the last 40 years, the synthesis and metabolism of vitamin D were elucidated and more than 50 metabolites of vitamin D have been discovered, though commercial measurement procedures have been developed for only a few of them. The clinical significance of vitamin D in calcium and phosphorus homeostasis is well appreciated. However, recent epidemiological dat...
Source: Hormones - January 11, 2020 Category: Endocrinology Tags: Hormones (Athens) Source Type: research
Vitamin D and Calcium Supplement Attenuate Bone Loss among HIV-infected Patients Receiving Tenofovir Disoproxil Fumarate/Emtricitabine/Efavirenz: An Open-label, randomized Controlled Trial.
CONCLUSION: Vitamin D2 and calcium supplements should be considered for HIV-infected patients receiving TDF/FTC/EFV in a resource-limited setting where there are limited ART options (Clinicaltrials.gov NCT0287643).
PMID: 31906840 [PubMed - as supplied by publisher] (Source: Current HIV Research)
Source: Current HIV Research - January 5, 2020 Category: Infectious Diseases Authors: Boontanondha P, Nimitphong H, Musikarat S, Ragkho A, Kiertiburanakul S Tags: Curr HIV Res Source Type: research
Quantitative analysis of vitamin D and its main metabolites in human milk by supercritical fluid chromatography coupled to tandem mass spectrometry.
Abstract
A novel method to quantitate vitamin D and its main metabolites (vitamin D3, vitamin D2, and their 25-hydroxy metabolites) in breast milk by supercritical fluid chromatography has been developed and fully validated. A small volume of sample (1 mL) is subjected to ethanolic protein precipitation and liquid-liquid extraction. Final extracts are derivatized with 4-phenyl-1,2,4-triazoline-3,5-dione and vitamin D derivatives analyzed by supercritical fluid chromatography hyphenated to tandem mass spectrometry with atmospheric pressure chemical ionization. Multiple reaction monitoring is used for quanti...
Source: Analytical and Bioanalytical Chemistry - December 11, 2019 Category: Chemistry Authors: Oberson JM, Bénet S, Redeuil K, Campos-Giménez E Tags: Anal Bioanal Chem Source Type: research
Molecules, Vol. 24, Pages 4517: Simultaneous LC/MS Analysis of Carotenoids and Fat-Soluble Vitamins in Costa Rican Avocados (Persea americana Mill.)
hilla
Avocado (a fruit that represents a billion-dollar industry) has become a relevant crop in global trade. The benefits of eating avocados have also been thoroughly described as they contain important nutrients needed to ensure biological functions. For example, avocados contain considerable amounts of vitamins and other phytonutrients, such as carotenoids (e.g., β-carotene), which are fat-soluble. Hence, there is a need to assess accurately these types of compounds. Herein we describe a method that chromatographically separates commercial standard solutions containing both fat-soluble vitamins (vitamin...
Source: Molecules - December 9, 2019 Category: Chemistry Authors: Carolina Cort és-Herrera Andrea Chac ón Graciela Artavia Fabio Granados-Chinchilla Tags: Article Source Type: research
Ergocalciferol Versus Cholecalciferol in Non-Dialysis Dependent Chronic Kidney Disease Patients: A Small Retrospective Cohort Study.
CONCLUSION: In this small pilot study, cholecalciferol treatment appeared to increase serum phosphorous concentrations compared to ergocalciferol. These observations may warrant further large-scale studies that are appropriately powered to validate such findings.
PMID: 31804923 [PubMed - in process] (Source: J Pharm Pharm Sci)
Source: J Pharm Pharm Sci - December 6, 2019 Category: Drugs & Pharmacology Authors: Al-Makki A, Frost K, Yun SA, Overholser B, Shepler B Tags: J Pharm Pharm Sci Source Type: research
Vitamin D and Health Outcomes
Not long ago, vitamin D was riding high. Beyond its role in calcium homeostasis and bone health, animal studies linked vitamin D deficiency to numerous chronic illnesses including hypertension, diabetes, autoimmunity, and malignancy. Corroborating human observational studies reported associations between vitamin D deficiency and increased risks of hypertension, diabetes, cardiovascular disease, autoimmunity, and cancer. The lay press seized on this chorus of observational studies, testing of serum 25-hydroxyvitamin D levels proliferated, and supplementation with cholecalciferol (vitamin D3) and ergocalciferol (vitamin D2) ...
Source: JAMA - Journal of the American Medical Association - November 8, 2019 Category: General Medicine Source Type: research
Enhancement of sorafenib-mediated death of Hepatocellular carcinoma cells by Carnosic acid and Vitamin D2 analog combination
Publication date: Available online 5 November 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Qunfeng Wu, Xuening Wang, Kien Pham, Aesis Luna, George P Studzinski, Chen LiuAbstractHepatocellular carcinoma (HCC) is the most common form of liver cancer and it is the third leading cause of global cancer mortality. Sorafenib (Sf) is the first oral multi-kinase inhibitor approved for systemic treatment of advanced HCC, and can prolong survival, although only for three months longer than placebo treated patients. Preclinical studies showed that active forms of vitamin D can induce cell differentia...
Source: The Journal of Steroid Biochemistry and Molecular Biology - November 6, 2019 Category: Biochemistry Source Type: research
Effects of UV-C treatment and ultrafine-grinding on the biotransformation of ergosterol to vitamin D2, physiochemical properties, and antioxidant properties of shiitake and Jew’s ear
Publication date: Available online 21 October 2019Source: Food ChemistryAuthor(s): Ziyun Xu, Maninder Meenu, Baojun XuAbstractIn the present study, the impact of ultraviolet (UV)-C treatment and ultrafine grinding on the conversion of ergosterol to vitamin D2, physiochemical properties, and antioxidant properties of shiitake and Jew’s ear was assessed. After exposure to UV-C vitamin D2 contents of both the mushroom samples has increased significantly (p < 0.05). Whereas, ultrafine grinding along with UV-C treatment has a synergistic effect on bioconversion of ergosterol to vitamin D2 and this effect is more prom...
Source: Food Chemistry - October 22, 2019 Category: Food Science Source Type: research
Toxicological evaluation of ergocalciferol, cholecalciferol, and their metabolites by a category approach
Volume 44, Issue 6, November 2021, Page 661-667 . (Source: Drug and Chemical Toxicology)
Source: Drug and Chemical Toxicology - August 15, 2019 Category: Toxicology Authors: Gulcin Tugcu Mohammad Charehsaz Ahmet Ayd ın Source Type: research
Toxicological evaluation of ergocalciferol, cholecalciferol, and their metabolites by a category approach
. (Source: Drug and Chemical Toxicology)
Source: Drug and Chemical Toxicology - August 14, 2019 Category: Toxicology Authors: Gulcin Tugcu Mohammad Charehsaz Ahmet Ayd ın Source Type: research
Phosphaturic Mesenchymal Tumor of Soft Tissue of the Foot: Report of a Case With Review of the Literature
We report a case of a PMT in a young adult male who presented in 2007 with the classic constellation of signs and symptoms. A small soft-tissue tumor was detected in his right heel, 3 years after exhaustively seeking for it by various imaging techniques performed at different institutions. Before the tumor was detected, attempts to manage this patient’s osteomalacia with phosphate and vitamin D (both calcitriol and ergocalciferol) supplementation were unsuccessful. Following surgical resection, the patient experienced prompt correction of the phosphaturia and gradual reconstitution of his bone mineralization. The patholo...
Source: Advances in Anatomic Pathology - August 9, 2019 Category: Pathology Tags: AMR Series Source Type: research
Differential response of lung cancer cell lines to vitamin D derivatives depending on EGFR, KRAS, p53 mutation status and VDR polymorphism
Publication date: Available online 18 July 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Ewa Maj, Justyna Trynda, Beata Maj, Katarzyna Gębura, Katarzyna Bogunia-Kubik, Michał Chodyński, Andrzej Kutner, Joanna WietrzykAbstractVitamin D reveals antiproliferative activity against many types of cancer cells. Calcitriol (1,25(OH)2D3), the most active form of vitamin D3, acts mainly through the vitamin D receptor, regulating the expression of target genes. Cells with reasonable expression of VDR are considered to be sensitive to antiproliferative activity of 1,25(OH)2D3. However, a few allele...
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 18, 2019 Category: Biochemistry Source Type: research
Vitamin D for skeletal and non-skeletal health: What we should know
Publication date: Available online 13 July 2019Source: Journal of Clinical Orthopaedics and TraumaAuthor(s): Nipith Charoenngam, Arash Shirvani, Michael F. HolickAbstractVitamin D plays an essential role in regulating calcium and phosphate metabolism and maintaining a healthy mineralized skeleton. Humans obtain vitamin D from sunlight exposure, dietary foods and supplements. There are two forms of vitamin D: vitamin D3 and vitamin D2. Vitamin D3 is synthesized endogenously in the skin and found naturally in oily fish and cod liver oil. Vitamin D2 is synthesized from ergosterol and found in yeast and mushrooms. Once vitamin...
Source: Journal of Clinical Orthopaedics and Trauma - July 14, 2019 Category: Orthopaedics Source Type: research
Molecules, Vol. 24, Pages 2369: Electrochemical Oxidation as a Tool for Generating Vitamin D Metabolites
Rohn
The electrochemical behavior of the vitamers cholecalciferol and ergocalciferol was investigated in order to determine whether it is possible to evaluate phase-I and phase-II metabolism of these steroids and yield metabolites that can serve as reference material. The vitamers were electrochemically-oxidized using an electrochemical system (ROXY&trade; EC system). The influence of pH value, solvent, and potential was evaluated. When using methanol or ethanol, the formation of artificial methoxy or ethoxy groups, respectively, was observed, while the use of acetonitrile did not show any formation of furt...
Source: Molecules - June 25, 2019 Category: Chemistry Authors: Navarro Suarez Thein Kallinich Rohn Tags: Article Source Type: research
