Investigation of vitamin D2 and vitamin D3 hydroxylation by Kutzneria albida
In this study, a novel strain, Kutzneria albida , was identified, showing the ability to form 25-OH-D 2 and 25-OH-D 3 . To further improve the conversion of the poorly soluble substrates, several solubilizers were tested. 100-fold higher product concentrations of 25-OH-D 3 and 10-fold higher concentrations of 25-OH-D 2 after addition of 5% (w/v) 2-hydroxypropyl β-cyclodextrin (2-HPβCD) were reached. Besides the single hydroxylation products, the human double hydroxylation products 1,25-(OH) 2 -D 2 and 1,25-(OH) 2 -D 3 and various other potential single and double hydroxylation products were detected. Thus, K. albida repr...
Source: Chembiochem - March 1, 2021 Category: Biochemistry Authors: Lisa Marie Schmitz Alina Kinner Kirsten Althoff Katrin Rosenthal Stephan L ütz Source Type: research
Investigation of vitamin D2 and vitamin D3 hydroxylation by Kutzneria albida
In this study, a novel strain, Kutzneria albida , was identified, showing the ability to form 25-OH-D 2 and 25-OH-D 3 . To further improve the conversion of the poorly soluble substrates, several solubilizers were tested. 100-fold higher product concentrations of 25-OH-D 3 and 10-fold higher concentrations of 25-OH-D 2 after addition of 5% (w/v) 2-hydroxypropyl β-cyclodextrin (2-HPβCD) were reached. Besides the single hydroxylation products, the human double hydroxylation products 1,25-(OH) 2 -D 2 and 1,25-(OH) 2 -D 3 and various other potential single and double hydroxylation products were detected. Thus, K. albida repr...
Source: Chembiochem - March 1, 2021 Category: Biochemistry Authors: Lisa Marie Schmitz Alina Kinner Kirsten Althoff Katrin Rosenthal Stephan L ütz Source Type: research
