G protein-coupled receptor 30 mediates meiosis resumption and gap junction communications downregulation in goat cumulus-oocyte complexes by 17β-estradiol
Publication date: Available online 8 November 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Hui Zhang, Qiang Wei, Zhen Gao, Chiyuan Ma, Zhenshan Yang, Hui Zhao, Chen Liu, Jie Liu, Xiaoe Zhao, Baohua MaAbstractEstrogen plays a critical role in the regulation of gap junctions between oocytes and granulosa cells in mammalian ovaries. G protein-coupled receptor 30 (GPR30) was identified as a membrane estrogen receptor, mediating rapid, nongenomic signaling events that might be responsible for the regulation of oocyte meiosis resumption and gap junction intercellular communications (GJICs). The...
Source: The Journal of Steroid Biochemistry and Molecular Biology - November 9, 2018 Category: Biochemistry Source Type: research

The Prevalence and risk factors of vitamin D inadequacy among male athletes in Kuwait: A cross-sectional study
Publication date: Available online 6 November 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Meshari J. Alkoot, Fiona Boland, Ruairi Brugha, Regien BiesmaAbstractThe aim of this study was to determine the prevalence of vitamin D inadequacy in male athletes living in Kuwait, and to assess its relationship with possible risk factors including body composition, lifestyle and fasting Ramadan. A Cross- sectional study was conducted on a total of 250 male athletes registered in official sports clubs in Kuwait were included. Blood tests, anthropometric measurements and a lifestyle questionnaire we...
Source: The Journal of Steroid Biochemistry and Molecular Biology - November 7, 2018 Category: Biochemistry Source Type: research

Determinants of vitamin D receptor gene expression in visceral and subcutaneous adipose tissue in non-obese, obese, and morbidly obese subjects
Publication date: Available online 6 November 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Emad Yuzbashian, Golaleh Asghari, Mehdi Hedayati, Maryam Zarkesh, Parvin Mirmiran, Alireza KhalajAbstractWe aimed to illustrate determinants of VDR gene expression in visceral and subcutaneous adipose tissue among individuals without diabetes. We gathered visceral and subcutaneous adipose tissues during an elective abdominal surgery form 33 morbidly obese (BMI > 40 kg/m2), 23 obese (BMI = 30–40 kg/m2), and 35 non-obese (BMI
Source: The Journal of Steroid Biochemistry and Molecular Biology - November 6, 2018 Category: Biochemistry Source Type: research

Association of Sun and UV exposure with Blood pressure and Cardiovascular Disease: A systematic review
Publication date: Available online 6 November 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Robert Scragg, Juma Rahman, Simon ThornleyABSTRACTBackgroundEcological studies show that sun or ultraviolet (UV) exposure have inverse associations with blood pressure (BP) and cardiovascular disease (CVD). Although sunlight is the primary source of vitamin D, recent meta-analyses of clinical trials have not reported a beneficial effect from vitamin D supplementation on BP or CVD. Sunlight may have beneficial effects independent of vitamin D. We carried out a systematic review to appraise the extent...
Source: The Journal of Steroid Biochemistry and Molecular Biology - November 6, 2018 Category: Biochemistry Source Type: research

Airway infection with Nontypeable Haemophilus influenzae is more rapidly eradicated in vitamin D deficient mice
Publication date: Available online 3 November 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Jef Serré, Carolien Mathyssen, Tom Tanjeko Ajime, Hannelie Korf, Karen Maes, Nele Heulens, Conny Gysemans, Chantal Mathieu, Bart Vanaudenaerde, Wim Janssens, Ghislaine Gayan-RamirezAbstractChronic obstructive pulmonary disease (COPD), which is characterized by an excessive inflammatory response of the airways, is often complicated by exacerbations. Vitamin D deficiency has been associated with an increased risk for COPD and may predispose COPD patients to a higher exacerbation rate, particula...
Source: The Journal of Steroid Biochemistry and Molecular Biology - November 4, 2018 Category: Biochemistry Source Type: research

25-hydroxycholesterol down-regulates oxysterol binding protein like 2 (OSBPL2) via the p53/SREBF2/NFYA signaling pathway
This study aimed to explore the molecular mechanism of 25-OHC regulating the expression of OSBPL2. Using dual-luciferase reporter assay, we found a decrease of nuclear transcription factor Y subunit alpha (NFYA) bound with OSBPL2 promoter when HeLa cells were treated with by 25-OHC. Furthermore, transcriptome sequencing and RNA interference results revealed that the p53/sterol regulatory element binding transcription factor 2 (SREBF2) signaling pathway was involved in the NFYA-dependent transcription of OSBPL2 induced by 25-OHC. Based on these results, we concluded that pleomorphic adenoma gene 1 (PLAG1) and NFYA participa...
Source: The Journal of Steroid Biochemistry and Molecular Biology - November 3, 2018 Category: Biochemistry Source Type: research

Contribution of nutrition science to the vitamin D field—clarity or confusion?
Publication date: Available online 2 November 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Kevin D. Cashman, Mairead KielyAbstractRecent opinion pieces have questioned whether nutrition science is fit for purpose, suggesting that the evidence-base for dietary recommendations is populated with poor quality science and unresolved controversy. Nutrition science is accused of not keeping up with the times and making little real-world progress to stem the growing global malnutrition crisis, by failing to apply cutting-edge techniques to nutrition problems. Nutritional epidemiology has been bla...
Source: The Journal of Steroid Biochemistry and Molecular Biology - November 3, 2018 Category: Biochemistry Source Type: research

Synthesis and characterization of 20-hydroxyvitamin D3 with the A-ring modification
Publication date: Available online 30 October 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Toshie Fujishima, Tsutomu Suenaga, Masatoshi Kawahata, Kentaro YamaguchiAbstractTwo novel 20-hydroxyvitamin D3 analogues (4a,b) with the A-ring modification have been synthesized by a convergent manner. An alternative pathway of vitamin D3 metabolism by cytochrome P450scc CYP11A1 was reported to afford 20-hydroxyvitamin D3 (3), functions of which remain to be explored. Based on the structure of the 20-hydroxy metabolite, novel analogues (4a,b) with the modifications, including the 1α-hydroxy, ...
Source: The Journal of Steroid Biochemistry and Molecular Biology - October 31, 2018 Category: Biochemistry Source Type: research

MicroRNA-128 is involved in dexamethasone-induced lipid accumulation via repressing SIRT1 expression in cultured pig preadipocytes
In this study, pig preadipocytes were firstly treated with 10-6 M DEX for 48 h to explore the role of dexamethasone (DEX, a chemically synthesized long-acting glucocorticoid) on lipid accumulation. Then, miRNA scrambled control (miR-SC), miR-128 overexpression plasmid and miR-128 inhibitor were respectively transfected into pig preadipocytes at 24 h before DEX treatment for 48 h (miR-SC-DEX, miR-128-DEX and miR-128-inhibitor-DEX) to illustrate the regulatory role of miR-128 on DEX-induced lipid accumulation. Compared with control preadipocytes, 10-6 M Dex significantly increased triglyceride (TG) level...
Source: The Journal of Steroid Biochemistry and Molecular Biology - October 29, 2018 Category: Biochemistry Source Type: research

A reduced protein diet modulates enzymes of vitamin D and cholesterol metabolism in young ruminants
Publication date: Available online 28 October 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Mirja R. Wilkens, Caroline S. Firmenich, Nadine Schnepel, Alexandra S. Muscher-BanseAbstractBesides other adverse effects, a low protein diet has been shown to modulate cholesterol and vitamin D metabolism in monogastric species like rats and humans. As ruminants can increase the efficiency of the rumino-hepatic circulation of urea, it is assumed that goats should be able to compensate for a low dietary protein intake better. After a dietary protein restriction (9% vs. 20%) for six weeks, plasma con...
Source: The Journal of Steroid Biochemistry and Molecular Biology - October 29, 2018 Category: Biochemistry Source Type: research

Neutrophils life under estrogenic and xenoestrogenic control
Publication date: Available online 28 October 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Karolina Nowak, Ewa Jabłońska, Wioletta Ratajczak-WronaAbstractOver 100 years ago, scientists had identified cells that represent the most abundant population of peripheral blood leukocytes; they called this population neutrophils. Day by day, the knowledge specific to neutrophils is augmented with new and often surprising aspects and facts about neutrophils’ life or death. Estrogens (estrone, estriol, and estradiol) are relevant for the regulation of immune responses that are related with n...
Source: The Journal of Steroid Biochemistry and Molecular Biology - October 29, 2018 Category: Biochemistry Source Type: research

Effects of lisdexamfetamine on plasma steroid concentrations compared with D-amphetamine in healthy subjects: a randomized, double-blind, placebo-controlled study
Publication date: Available online 28 October 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Petra Strajhar, Patrick Vizeli, Melanie Patt, Patrick C. Dolder, Denise V. Kratschmar, Matthias E. Liechti, Alex OdermattAbstractThe novel D-amphetamine prodrug lisdexamfetamine is applied to treat attention-deficit/hyperactivity disorder (ADHD). D-amphetamine releases dopamine and norepinephrine and stimulates the hypothalamic-pituitary-adrenal (HPA) axis, which may contribute to its reinforcing effects and risk of abuse. However, no data is currently available on the effects of lisdexamfetamine on...
Source: The Journal of Steroid Biochemistry and Molecular Biology - October 29, 2018 Category: Biochemistry Source Type: research

A randomised controlled trial of vitamin D and omega-3 long chain polyunsaturated fatty acids in the treatment of irritability and hyperactivity among children with Autism Spectrum Disorder
Publication date: Available online 26 October 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Hajar Mazahery, Cathryn A. Conlon, Kathryn L. Beck, Owen Mugridge, Marlena C. Kruger, Welma Stonehouse, Carlos A. Camargo, Barbara J. Meyer, Beatrix Jones, Pamela R. von HurstAbstractIrritability and hyperactivity are common in children with Autism Spectrum Disorder (ASD). Because pharmacological treatments may have adverse effects, and despite limited evidence, caregivers/parents often use dietary supplements such as vitamin D and omega-3 fatty acids to address these behavioural symptoms. As a seco...
Source: The Journal of Steroid Biochemistry and Molecular Biology - October 28, 2018 Category: Biochemistry Source Type: research

Identification of the metabolic fingerprints in women with polycystic ovary syndrome using the multiplatform metabolomics technique
Publication date: Available online 27 October 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Magdalena Buszewska-Forajta, Dominik Rachoń, Anna Stefaniak, Renata Wawrzyniak, Aleksandra Konieczna, Agnieszka Kowalewska, Michał Jan MarkuszewskiABSTRACTIn addition to chronic anovulation and clinical signs of hyperandrogenism women with polycystic ovary syndrome (PCOS) are insulin resistant and therefore, develop central obesity with its long term consequences such as dyslipidaemia, hypertension, atherosclerosis and type 2 diabetes mellitus (T2DM), which all lead to the development of cardiovas...
Source: The Journal of Steroid Biochemistry and Molecular Biology - October 28, 2018 Category: Biochemistry Source Type: research

Concise Synthesis of 23-Hydroxylated Vitamin D3 Metabolites
Publication date: Available online 24 October 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Fumihiro Kawagoe, Toru Sugiyama, Kaori Yasuda, Motonari Uesugi, Toshiyuki Sakaki, Atsushi KittakaAbstractThree 23-hydroxylated vitamin D3 derivatives, which are metabolites of 25-hydroxyvitamin D3 produced by CYP24A1 and a related diastereomer, were efficiently synthesized. Each C23 hydroxy unit was constructed by the Claisen condensation reaction with ethyl acetate or the Grignard reaction with 2-methylallymagnesium chloride. Stereochemistry at the C23 position was determined by a modified Mosher&r...
Source: The Journal of Steroid Biochemistry and Molecular Biology - October 25, 2018 Category: Biochemistry Source Type: research

Effect of 16-weeks vitamin D replacement on calcium-phosphate homeostasis in overweight and obese adults
Publication date: Available online 25 October 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Jakub Mesinovic, Aya Mousa, Kirsty Wilson, Robert Scragg, Magdalena Plebanski, Maximilian de Courten, David Scott, Negar Naderpoor, Barbora de CourtenAbstractThis randomised placebo-controlled trial aimed to determine the effect of 16-weeks cholecalciferol supplementation on calcium-phosphate homeostasis and bone mineral density (BMD) in overweight and obese adults. Fifty-four vitamin D-deficient (25OHD
Source: The Journal of Steroid Biochemistry and Molecular Biology - October 25, 2018 Category: Biochemistry Source Type: research

Negative correlation between testosterone and TNF-α in umbilical cord serum favors a weakened immune milieu in the human male fetoplacental unit
Publication date: Available online 22 October 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Andrea Olmos-Ortiz, Janice García-Quiroz, Ali Halhali, Euclides Avila, Verónica Zaga-Clavellina, Roberto Chavira-Ramírez, Rocío García-Becerra, Felipe Caldiño-Soto, Fernando Larrea, Lorenza DíazAbstractClinical and epidemiological evidence supports that pregnancies carrying a male fetus are more vulnerable to infections and preterm birth, probably due to testosterone immunosuppressive properties. In human placentas, testosterone lowers the expression ...
Source: The Journal of Steroid Biochemistry and Molecular Biology - October 24, 2018 Category: Biochemistry Source Type: research

Vitamin D effects on sphingosine 1-phosphate signaling and metabolism in monocytes from type 2 diabetes patients and controls
Publication date: Available online 15 October 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Nojan Nejatian, Sandra Trautmann, Dominique Thomas, Josef Pfeilschifter, Klaus Badenhoop, Alexander Koch, Marissa Penna-MartinezABSTRACTElevated sphingosine 1-phopshate (S1P) concentration was observed in type 2 diabetes mellitus (T2D). On the other side, 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3) can influence the formation of sphingosine 1-phopshate (S1P) and the expression of S1P receptors, which are known to be involved in T2D. In order to evaluate mechanisms for the antiinflammatory potentia...
Source: The Journal of Steroid Biochemistry and Molecular Biology - October 15, 2018 Category: Biochemistry Source Type: research

Vitamin D supplementation improves waist-to-hip ratio and fasting blood glucose in vitamin D deficient, overweight or obese Asians: A pilot secondary analysis of a randomised controlled trial
Publication date: Available online 12 October 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): David Scott, Aya Mousa, Negar Naderpoor, Maximilian P.J. de Courten, Robert Scragg, Barbora de CourtenAbstractRecent trials do not support a role for vitamin D supplementation in prevention or treatment of type 2 diabetes mellitus, although effects may differ in Asian populations. In this pilot secondary analysis of a placebo-controlled randomised trial of overweight or obese individuals with low 25-hydroxyvitamin D (25(OH)D
Source: The Journal of Steroid Biochemistry and Molecular Biology - October 13, 2018 Category: Biochemistry Source Type: research

Discovery of New Non-steroidal Selective Glucocorticoid Receptor Agonists
Publication date: Available online 12 October 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Constantinos Potamitis, Dimitra Siakouli, Konstantinos D. Papavasileiou, Athina Boulaka, Vassiliki Ganou, Marina Roussaki, Theodora Calogeropoulou, Panagiotis Zoumpoulakis, Michael N. Alexis, Maria Zervou, Dimitra J. MitsiouAbstractGlucocorticoids (GCs) are widely used as potent anti-inflammatory drugs; however, GC therapy is often accompanied by adverse side effects. The anti-inflammatory action of GCs is exerted through the glucocorticoid receptor (GR) in part by antagonizing the pro-inflammatory ...
Source: The Journal of Steroid Biochemistry and Molecular Biology - October 13, 2018 Category: Biochemistry Source Type: research

Antineoplastic effect of 1α,25(OH)2D3 in spheroids from endothelial cells transformed by Kaposi’s sarcoma-associated herpesvirus G protein coupled receptor
In conclusion, these data have revealed that 1α,25(OH)2D3 inhibits vGPCR MCS proliferation and induces apoptosis similar to vGPCR cells growing in 2D-cultures. (Source: The Journal of Steroid Biochemistry and Molecular Biology)
Source: The Journal of Steroid Biochemistry and Molecular Biology - October 9, 2018 Category: Biochemistry Source Type: research

Avoiding maternal vitamin D deficiency may lower blood glucose in pregnancy
ConclusionAvoiding maternal vitamin D deficiency in early pregnancy is associated with lower blood glucose in early pregnancy and throughout pregnancy. Higher 25OHD in late pregnancy was associated with higher odds of LGA at birth. (Source: The Journal of Steroid Biochemistry and Molecular Biology)
Source: The Journal of Steroid Biochemistry and Molecular Biology - October 8, 2018 Category: Biochemistry Source Type: research

Laboratory Trend in Vitamin D Status in Ireland: Dual Concerns about Low and High 25OHD
In this study, all 25OHD results from 2014 to 2016 were retrieved (n = 67,922) and trimmed to 40,307 results after duplicates were excluded. The average monthly actual 25OHD was slightly higher than the average monthly forecast 25OHD (p = 0.028) with a strong correlation between the actual 25OHD and forecast 25OHD (r = 0.79, p 
Source: The Journal of Steroid Biochemistry and Molecular Biology - October 6, 2018 Category: Biochemistry Source Type: research

Vitamin D C3-epimer levels are proportionally higher with oral vitamin D supplementation compared to ultraviolet irradiation of skin in mice but not humans
This study aimed to compare the effect of exposure to ultraviolet radiation (UVR) and oral vitamin D3 supplementation on vitamin D C3-epimer levels. C57Bl/6 female mice were fed either vitamin D-sufficient (vitamin D3 2000 IU/kg) or -deficient diets (no vitamin D3) for 4 weeks. Among the vitamin D-deficient group, the shaved backs of half were irradiated daily for 4 days with 1 kJ/m2 UVR, followed by twice weekly irradiation for 4 weeks. Despite similar 25(OH)D3 levels, the UV-irradiated group had a lower proportion of C3-epi 25(OH)D3 at week 7 (p 
Source: The Journal of Steroid Biochemistry and Molecular Biology - October 6, 2018 Category: Biochemistry Source Type: research

Chitosan-eurycomanone nanoformulation acts on steroidogenesis pathway genes to increase the reproduction rate in fish
Publication date: Available online 22 September 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Irfan Ahmad Bhat, Irshad Ahmad, Ishfaq Nazir Mir, Raja Aadil Hussain Bhat, Gireesh-Babu P, Mukunda Goswami, Sundaray J.K., Rupam SharmaAbstractThe study was undertaken to explore the molecular mechanism of eurycomanone, a major compound of Eurycoma longifolia plant in increasing the reproductive processes in the male fish model. Chitosan-nanoconjugated eurycomanone nanoparticles with a significant particle size [130 nm (CED1); 144.1 nm (CED2)] and stable zeta potentials (+49.1&thinsp...
Source: The Journal of Steroid Biochemistry and Molecular Biology - October 5, 2018 Category: Biochemistry Source Type: research

Synthesis of 1α,25-dihydroxyvitamin D3 analogues with α,α-difluorocycloketone at the CD-ring side chains and their biological properties in ovariectomized rats
Publication date: Available online 22 September 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Wei Wang, Guo-Dong Zhao, Ying-jie Cui, Min-Qi Li, Zhao-Peng LiuAbstractTwo novel 1α,25-dihydroxyvitamin D3 derivatives containing a α,α-difluorocyclopentanone (3) or α,α-difluorocyclohexanone (4) moiety at the CD-ring side chains were designed, synthesized, and evaluated for their biological properties on restoring bone mass in ovariectomized (OVX) rats with established osteopenia. The synthesis of compounds 3 and 4 utilized the Wittig-Horner coupling to build up the ...
Source: The Journal of Steroid Biochemistry and Molecular Biology - October 5, 2018 Category: Biochemistry Source Type: research

GPER and ERα mediate estradiol enhancement of mitochondrial function in inflamed adipocytes through a PKA dependent mechanism
In conclusionE2 prevents IL6 induced inflammation in adipocytes and promotes mitochondrial function through the combined activation of both GPER and ERα. These findings expand our understanding of ER interactions under inflammatory conditions in female rodent white adipose tissue.Graphical asbtract (Source: The Journal of Steroid Biochemistry and Molecular Biology)
Source: The Journal of Steroid Biochemistry and Molecular Biology - October 5, 2018 Category: Biochemistry Source Type: research

Paramount importance of sample quality in pre-clinical and clinical research – need for standard operating procedures (SOPs)
Publication date: Available online 24 September 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Tea Lanisnik Rizner, Jerzy Adamski (Source: The Journal of Steroid Biochemistry and Molecular Biology)
Source: The Journal of Steroid Biochemistry and Molecular Biology - October 5, 2018 Category: Biochemistry Source Type: research

How high-resolution techniques enable reliable steroid identification and quantification
Publication date: Available online 27 September 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Andrea Bileck, Christa E. Fluck, Nasser Dhayat, Michael GroesslAbstractDue to possible matrix interferences and artefact generation during sample preparation, careful method validation is required for quantitative bioanalytical methods, especially for analytes that are only present in low concentrations. Using the identification and quantification of progesterone metabolite in the urine of newborns as an example, we show how modern high-resolution instruments can be used to verify analyte assignme...
Source: The Journal of Steroid Biochemistry and Molecular Biology - October 5, 2018 Category: Biochemistry Source Type: research

Ligand chirality can affect histidine protonation of vitamin-D receptor: ab initio molecular orbital calculations in water
Publication date: Available online 29 September 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Yuta Terauchi, Rie Suzuki, Ryosuke Takeda, Ittetsu Kobayashi, Atsushi Kittaka, Midori Takimoto-Kamimura, Noriyuki KuritaAbstractVitamin D is recognized to play important roles in the onset of immunological diseases as well as the regulation of the amount of Ca in the blood. Since these physiological actions caused by active vitamin D are triggered by the specific interaction between the vitamin D receptor (VDR) and active vitamin D, many types of compounds have been developed as potent ligands aga...
Source: The Journal of Steroid Biochemistry and Molecular Biology - October 5, 2018 Category: Biochemistry Source Type: research

Longitudinal Changes in Serum Vitamin D Binding Protein and Free 25-hydroxyvitamin D in a Multiracial Cohort of Pregnant Adolescents
Publication date: Available online 29 September 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Cora M Best, Eva K Pressman, Ruth Anne Queenan, Elizabeth Cooper, Kimberly O O’BrienAbstractSerum free 25-hydroxyvitamin D (25(OH)D) rather than total 25(OH)D may better indicate vitamin D status during pregnancy given the pregnancy-associated increase in serum vitamin D binding protein (DBP) concentration. Our aims were to assess changes in DBP and free 25(OH)D across gestation and to determine whether free compared with total 25(OH)D more strongly correlates with markers of vitamin D and c...
Source: The Journal of Steroid Biochemistry and Molecular Biology - October 5, 2018 Category: Biochemistry Source Type: research

Effects of the pesticide chlorpyrifos on breast cancer disease. implication of epigenetic mechanisms
Publication date: Available online 2 October 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): C. Ventura, C.D. Zappia, M. Lasagna, W. Pavicic, S. Richard, A.D. Bolzan, F. Monczor, M. Núñez, C. CoccaAbstractChlorpyrifos (CPF) is an organophosphorus pesticide used for agricultural pest control all over the world. We have previously demonstrated that environmental concentrations of this pesticide alter mammary gland histological structure and hormonal balance in rats chronically exposed. In this work, we analyzed the effects of CPF on mammary tumors development. Our results demonst...
Source: The Journal of Steroid Biochemistry and Molecular Biology - October 5, 2018 Category: Biochemistry Source Type: research

The Intrinsically Disordered C-terminal F Domain of the Ecdysteroid Receptor from Aedes aegypti Exhibits Metal Ion-binding Ability
Publication date: Available online 20 September 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Anna Więch, Magdalena Rowińska-Żyrek, Joanna Wątły, Aleksandra Czarnota, Rafał Hołubowicz, Zbigniew Szewczuk, Andrzej Ożyhar, Marek OrłowskiAbstractThe dominant vector of dengue and Zika diseases is a female Aedes aegypti mosquito. Its reproduction is controlled by the formation of an active heterodimer complex of the 20-hydroxyecdysone receptor (EcR) and Ultraspiracle protein (Usp). Although EcR exhibits a structural and functional organization typical of nuclear receptors (NRs), the EcR...
Source: The Journal of Steroid Biochemistry and Molecular Biology - September 22, 2018 Category: Biochemistry Source Type: research

Synthesis of 28,28,28-trideutero-25-hydroxydihydrotachysterol2
Publication date: Available online 19 September 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Rita Sigüeiro, Julian Loureiro, Patricia González-Berdullas, Antonio Mouriño, Miguel A. MaestroAbstractAs part of our program on synthesis of labeled vitamin D metabolites and analogs, we describe here an efficient and versatile synthetic approach to 28,28,28-trideutero- 25-hydroxydihydrotachysterol2 where isotopic labeling was incorporated stereoselectively in the last step of the synthesis. This deuterated compound will allow the study this analog in vitro or in vivo and to me...
Source: The Journal of Steroid Biochemistry and Molecular Biology - September 20, 2018 Category: Biochemistry Source Type: research

Synthesis of 19-norcalcitriol analogs with pegylated alkylidene chains at C-2
Publication date: Available online 20 September 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Adrian Fabisiak, Pawel Brzeminski, Klaudia Berkowska, Ewa Marcinkowska, Rafal R. SicinskiAbstractThe results presented in this paper constitute an extension of our synthetic efforts focused on 19-norvitamin D compounds possessing elongated 2-alkylidene substituents. Based on our previous results, molecular modeling studies, and docking experiments, we selected a novel 19-norcalcitriol analog with long chain at C-2 containing several ether moieties and terminated by 2-(pyridin-2’-yl)ethylamin...
Source: The Journal of Steroid Biochemistry and Molecular Biology - September 20, 2018 Category: Biochemistry Source Type: research

Localization of the 1,25-dihydroxyvitamin D-mediated response in the intestines of mice
Publication date: Available online 17 September 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Carmen J. Reynolds, Nicholas J. Koszewski, Ronald L. Horst, Donald C. Beitz, Jesse P. GoffABSTRACT1,25-Dihydroxyvitamin D3 (1,25(OH)2D) elicits a transcriptional response in the intestines. Assessments of this response are often derived from crude tissue homogenates and eliminate the ability to discriminate among different cell types. Here, we used an RNA in situ hybridization assay, RNAScope (Advanced Cell Diagnostics, Newark, CA), to identify the cells in the intestine that respond to 1,25(OH)2D...
Source: The Journal of Steroid Biochemistry and Molecular Biology - September 18, 2018 Category: Biochemistry Source Type: research

Estrogen and progesterone dependent expression of visfatin/NAMPT regulates proliferation and apoptosis in mice uterus during estrous cycle
This study showed that expression of visfatin protein is steroid dependent in mouse uterus which is involved in the regulation of proliferation and apoptosis via modulating antioxidant system in the uterus of mice during the reproductive cycle.Graphical abstract (Source: The Journal of Steroid Biochemistry and Molecular Biology)
Source: The Journal of Steroid Biochemistry and Molecular Biology - September 16, 2018 Category: Biochemistry Source Type: research

The dual sex hormone specificity for human reductive 17β-hydroxysteroid dehydrogenase type 7: synergistic function in estrogen and androgen control
Publication date: Available online 15 September 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Jean-François Thériault, Sheng-Xiang LinAbstractHuman 17β-hydroxysteroid dehydrogenase (17β-HSD) type 1 and 7 catalyze the final step of estrogen activation and the first step in androgen inactivation. It has been shown in breast cancer cells that DHT has a suppression effect on cell proliferation, counteracting the estrogen growth effect. However, the exact kinetic function of 17β-HSD7 in steroidogenesis was not determined. Here we report the steady-state kinetics and...
Source: The Journal of Steroid Biochemistry and Molecular Biology - September 16, 2018 Category: Biochemistry Source Type: research

Lifetime risk of cardiometabolic mortality according to vitamin D status of middle and older-aged adults: NHANES III mortality follow-up
In this study, we followed up 7,958 participants in the Third National Health and Nutrition Examination Survey from 1988-1994 (NHANES III) until the occurrence of cardiometabolic death or attainment of 95 years of age (median follow-up 17.9 years, 1,371 cardiometabolic-deaths). Lifetime risks were estimated according to recommended total 25(OH)D cutoffs by national guidelines, and a combination of total 25(OH)D status and traditional risk factor burden. We also explored variation in lifetime risk estimates by levels of body mass index (BMI). The results of this study showed that annualized total 25(OH)D
Source: The Journal of Steroid Biochemistry and Molecular Biology - September 14, 2018 Category: Biochemistry Source Type: research

The serum vitamin D metabolome: What we know and what is still to discover
Publication date: Available online 8 September 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Robert C Tuckey, Chloe Y. S Cheng, Andrzej T SlominskiAbstractVitamin D, referring to the two forms, D2 from the diet and D3 primarily derived from phototransformation in the skin, is a prohormone important in human health. The most hormonally active form, 1α,25-dihydroxyvitamin D (1α,25(OH)2D), formed from vitamin D via 25-hydroxyvitamin D (25(OH)D), is not only important for regulating calcium metabolism, but has many pleiotropic effects including regulation of the immune system and h...
Source: The Journal of Steroid Biochemistry and Molecular Biology - September 9, 2018 Category: Biochemistry Source Type: research

The Characterization of Androstenone Transport in Boar Plasma
Publication date: Available online 8 September 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Christine Bone, Courtney Anderson, Yanping Lou, E.J. SquiresAbstractThe transport of steroids by plasma proteins influences the amount of steroid available for uptake by the target tissue. In the boar, androstenone is transported to the adipose tissue where it accumulates to cause an off-odour or off-flavour in pork, known as boar taint. The mechanism of the transport of androstenone in the boar remains unclear, and the plasma protein responsible for binding androstenone has yet to be identified. T...
Source: The Journal of Steroid Biochemistry and Molecular Biology - September 9, 2018 Category: Biochemistry Source Type: research

Vitamin D supplementation may improve back pain disability in vitamin D deficient and overweight or obese adults
Publication date: Available online 7 September 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Sharmayne R.E. Brady, Negar Naderpoor, Maximilian P.J. de Courten, Robert Scragg, Flavia Cicuttini, Aya Mousa, Barbora de CourtenAbstractBack pain is currently the greatest cause of disability worldwide, and there are very limited therapeutic options available. Vitamin D deficiency and obesity are both risk factors for back pain. The few randomised controlled trials examining the effects of vitamin D supplementation on back pain have methodological limitations and largely include non-vitamin D defi...
Source: The Journal of Steroid Biochemistry and Molecular Biology - September 8, 2018 Category: Biochemistry Source Type: research

Molecular identification of steroidogenesis-related genes in scallops and their potential roles in gametogenesis
In this study, we characterized several steroidogenesis-related genes in M. yessoensis and proposed a putative biosynthetic pathway for sex steroids that is similar to that of vertebrates. Specifically, we identified several steroidogenesis-related gene sequences that encode steroid metabolizing enzymes: StAR-related lipid transfer (START) protein, 17α-hydroxylase, 17,20-lyase (cyp17a), 17β-hydroxysteroid dehydrogenase (hsd17b), and 3β-hydroxysteroid dehydrogenase (hsd3b). We sampled adult scallops throughout their reproductive phase to compare their degree of maturation with their intensity of mRNA express...
Source: The Journal of Steroid Biochemistry and Molecular Biology - September 7, 2018 Category: Biochemistry Source Type: research

1,25-dihydroxyvitamin D3 modulates the effects of sublethal BPA on mitochondrial function via activating PI3K-Akt pathway and 17β-estradiol secretion in rat granulosa cells
Publication date: Available online 5 September 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Ching-Tien Lee, Jiz-Yuh Wang, Kuang-Yi Chou, Ming-I HsuAbstractBisphenol A (BPA), an endocrine-disrupting chemical, is capable of producing reproductive toxicity. BPA results in mitochondrial DNA (mtDNA) deletion and mitochondrial dysfunction; however, the effect of BPA on the mitochondria of ovarian granulosa cells is not clear. Further, 1,25-dihydroxyvitamin D3 (1,25D3) may play a role in reproduction, because its receptor, VDR, contributes to the inhibition of oxidative stress and predominantly ...
Source: The Journal of Steroid Biochemistry and Molecular Biology - September 6, 2018 Category: Biochemistry Source Type: research

Virtual screening and drug repositioning as strategies for the discovery of new antifungal inhibitors of oxidosqualene cyclase
Publication date: Available online 5 September 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Vitor Won-Held Rabelo, Daiane de Jesus Viegas, Erline Machado Neves Tucci, Nelilma Correia Romeiro, Paula Alvarez AbreuABSTRACTCandidiasis is the most common fungal infection in immunocompromised patients, and Candida albicans is the fourth leading agent of nosocomial infections. Mortality from this infection is significant; however, the therapeutic treatment is limited, which demands the search for new drugs and new targets. In this context, oxidosqualene cyclase (OSC) catalyzes the cyclization of...
Source: The Journal of Steroid Biochemistry and Molecular Biology - September 5, 2018 Category: Biochemistry Source Type: research

Editorial Board
Publication date: October 2018Source: The Journal of Steroid Biochemistry and Molecular Biology, Volume 183Author(s): (Source: The Journal of Steroid Biochemistry and Molecular Biology)
Source: The Journal of Steroid Biochemistry and Molecular Biology - September 4, 2018 Category: Biochemistry Source Type: research

So similar and so different: Circulating androgens and androgen origin in bulimic women
The objective of the study was to determine whether androgen levels and androgen origin differs in bulimic women compared to control subjects.Forty-six women with bulimia nervosa (BN), 31 with eating disorder not otherwise specified, purging type (EDNOS P) and 56 matched healthy controls were studied with respect to serum testosterone (T), 5alpha-dihydrotestosterone (DHT), sex hormone-binding globulin (SHBG), deyhydroepiahndrosterone sulfate (DHEAS) and luteinizing hormone (LH) and to ovarian morphology.Despite all groups had almost identical androgen and SHBG levels; there were differences in the origin of circulating T a...
Source: The Journal of Steroid Biochemistry and Molecular Biology - August 31, 2018 Category: Biochemistry Source Type: research

The crustacean ecdysone cassette: A gatekeeper for molt and metamorphosis
Publication date: Available online 26 August 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Cameron J. Hyde, Abigail Elizur, Tomer VenturaAbstractArthropods have long been utilized as models to explore molecular function, and the findings derived from them can be applied throughout metazoa, including as a basis for medical research. This has led to the adoption of many representative insect models beyond Drosophila, as each lends its own unique perspective to questions in endocrinology and genetics. However, non-insect arthropods are yet to be realised for the potential insight they may pro...
Source: The Journal of Steroid Biochemistry and Molecular Biology - August 27, 2018 Category: Biochemistry Source Type: research

Glucocorticoid-induced insulin resistance is related to macrophage visceral adipose tissue infiltration
Publication date: Available online 23 August 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Thi Thu Huong Do, Garcia Marie, Dalle Héloïse, Dorothée Guillaume, Moldes Marthe, Fève Bruno, Buyse MarionAbstractInsulin resistance is frequently present in patients with glucocorticoid (GC) excess (Cushing’s syndrome) or treated with high doses of GCs. Furthermore, others similarities between metabolic syndrome (visceral obesity, elevated blood glucose levels, dyslipidemia) and Cushing’s syndrome suggest that GCs could play a role in obesity-linked complication...
Source: The Journal of Steroid Biochemistry and Molecular Biology - August 24, 2018 Category: Biochemistry Source Type: research

Progesterone up-regulates p27 through an increased binding of the progesterone receptor-A-p53 protein complex onto the non-canonical p53 binding motif in HUVEC
In this study, we identified three putative p53 protein binding domains at the p27 promoter. Luciferase assay showed that the activity of ectopically introduced p27 promoter constructs containing the potential p53 protein binding region was significantly increased by P4. Immunoblotting analysis indicated that P4 increased the level of p53 protein. Treatment with pifithrin-α-HBr (PFTα), a specific blocker of p53-responsive gene transactivation, reduced the P4-increased p27 promoter activity and p27 protein expression. Transfection with dominant-negative mutants of p53 (C135Y, R175H and R248 W) abolished t...
Source: The Journal of Steroid Biochemistry and Molecular Biology - August 24, 2018 Category: Biochemistry Source Type: research