The promiscuous estrogen receptor: Evolution of physiological estrogens and response to phytochemicals and endocrine disruptors
Publication date: Available online 20 July 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Michael E. Baker, Richard LatheAbstractMany actions of estradiol (E2), the principal physiological estrogen in vertebrates, are mediated by estrogen receptor-α (ERα) and ERβ. An important physiological feature of vertebrate ERs is their promiscuous response to several physiological steroids, including estradiol (E2), Δ5-androstenediol, 5α-androstanediol, and 27-hydroxycholesterol. A novel structural characteristic of Δ5-androstenediol, 5α-androstanediol, and 27-...
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 20, 2018 Category: Biochemistry Source Type: research

Cerebroprotection by progesterone following ischemic stroke: multiple effects and role of the neural progesterone receptors
Publication date: Available online 19 July 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Xiaoyan Zhu, Magalie Fréchou, Michael Schumacher, Rachida GuennounABSTRACTTreatment with progesterone limits brain damage after stroke. However, the cellular bases of the cerebroprotective effects of progesterone are not well documented. The aims of this study were to determine neural cells and functions that are affected by progesterone treatment and the role of neural progesterone receptors (PR) after stroke. Adult male PRNesCre mice, selectively lacking PR in the central nervous system, and t...
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 20, 2018 Category: Biochemistry Source Type: research

Initial characterization of human DHRS1 (SDR19C1), a member of the short-chain dehydrogenase/reductase superfamily
Publication date: Available online 18 July 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Zemanová Lucie, Navrátilová Hana, Andrýs Rudolf, Šperková Kristýna, Andrejs Jiří, Kozáková Klára, Marc Meier, Gabriele Möller, Eva Novotná, Šafr Miroslav, Jerzy Adamski, Wsól VladimírAbstractMany enzymes from the short-chain dehydrogenase/reductase superfamily (SDR) have already been well characterized, particularly those that participate in crucial biochemical reactions in the human body (e.g...
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 19, 2018 Category: Biochemistry Source Type: research

Characterizing the Steroidal Milieu in Amniotic Fluid of Mid-Gestation: A LC-MS/MS Study
Publication date: Available online 18 July 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): R. Wang, D. Tiosano, A. Sánchez-Guijo, M.F. Hartmann, S.A. WudyAbstractGrowth and development of an embryo or fetus during human pregnancy mainly depend on intact hormone biosynthesis and metabolism in maternal amniotic fluid (AF). We investigated the hormonal milieu in AF and developed a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the determination of 14 sulfated and 6 unconjugated steroids in AF. 65 A F samples (male: female = 35: 30) of mid-gestation ranging f...
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 19, 2018 Category: Biochemistry Source Type: research

Generation of 1,25-dihydroxyvitamin D3 in Cyp27b1 knockout mice by treatment with 25-hydroxyvitamin D3 rescued their rachitic phenotypes
Publication date: Available online 18 July 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Miyu Nishikawa, Kaori Yasuda, Masashi Takamatsu, Keisuke Abe, Kimie Nakagawa, Naoko Tsugawa, Yoshihisa Hirota, Kazuma Tanaka, Shigeaki Yamashita, Shinichi Ikushiro, Tatsuo Suda, Toshio Okano, Toshiyuki SakakiAbstractWe have reported that 25-hydroxyvitamin D3 [25(OH)D3] binds to vitamin D receptor and exhibits several biological functions directly in vitro. To evaluate the direct effect of 25(OH)D3 in vivo, we used Cyp27b1 knockout (KO) mice, which had no detectable plasma 1α,25-dihydroxyvitamin D...
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 19, 2018 Category: Biochemistry Source Type: research

Identification of 9-oxo-1,2,3,4,5,6,10,19-octanor-13,17-secoandrost-8(14)-ene-7,17-dioic acid as a metabolite of steroid degradation in Comamonas testosteroni TA441 and the genes involved in the conversion
Publication date: Available online 17 July 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Masae Horinouchi, Hiroyuki Koshino, Michal Malon, Hiroshi Hirota, Toshiaki HayashiABSTRACTComamonas testosteroni TA441 degrades steroid compounds via aromatization of the A-ring to produce 9,17-dioxo-1,2,3,4,10,19-hexanorandrostan-5-oic acid (a metabolite with C- and D-rings), which is presumed to be further degraded via β-oxidation. In elucidating the complete steroid degradation process in C. testosteroni, we isolated 9-oxo-1,2,3,4,5,6,10,19-octanor-13,17-secoandrost-8(14)-ene-7,17-dioic acid an...
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 18, 2018 Category: Biochemistry Source Type: research

Identification of 4-methyl-5-oxo-octane-1,8-dioic acid and the derivatives as metabolites of steroidal C,D-ring degradation in Comamonas testosteroni TA441
In this study, we isolated and identified 4-methyl-5-oxo-octane-1,8-dioic acid and 4-methyl-5-oxo-3-octene-1,8-dioic acid from the culture of the ORF3 (scdN)-null mutant as metabolites of steroid degradation (ADD and cholic acid analogues; cholic acid, chenodeoxycholic acid, deoxycholic acid, and lithocholic acid). In addition of these compounds, UHPLC/MS analysis of the culture of the scdN-null mutant revealed significant accumulation of another compound, which was detected as a dominant peak of m/z 155 ([M-CO2]-) accompanied by a small peak of parental ion (m/z 199 [M-]). On the bases of experimental data, this compound ...
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 18, 2018 Category: Biochemistry Source Type: research

Fatty-acylation target sequence in the ligand-binding domain of vertebrate steroid receptors demarcates evolution from estrogen-related receptors
We report that target sequences for fatty-acylation (palmitoylation) at a key cysteine residue (corresponding to Cys447 in human estrogen receptor ERα) in helix 8 of the ligand-binding domain accurately demarcate SRs from ERRs. Docking studies are consistent with the hypothesis that palmitate embeds into a key groove in the receptor surface. The implications of lipidation, and of potential alternative ligands for the key cysteine residue, for receptor function and the evolution of SRs are discussed.Graphical abstract (Source: The Journal of Steroid Biochemistry and Molecular Biology)
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 18, 2018 Category: Biochemistry Source Type: research

Ecdysteroid and juvenile hormone biosynthesis, receptors and their signaling in the freshwater microcrustacean Daphnia
Publication date: Available online 13 December 2017Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Hitoshi Miyakawa, Tomomi Sato, You Song, Knut Erik Tollefsen, Taisen IguchiAbstractThe two essential insect hormones, ecdysteroids and juvenile hormones, are possessed not only by insects, but also widely by arthropods, and regulate various developmental and physiological processes. In contrast to the abundant information about molecular endocrine mechanisms in insects, the knowledge of non-insect arthropod endocrinology is still limited. In this review, we summarize recent reports about the molecu...
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 11, 2018 Category: Biochemistry Source Type: research

It is high time to discontinue use of misidentified and contaminated cells: Guidelines for description and authentication of cell lines
Publication date: Available online 29 December 2017Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Tea Lanišnik Rižner, Jerzy Adamski (Source: The Journal of Steroid Biochemistry and Molecular Biology)
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 11, 2018 Category: Biochemistry Source Type: research

Juvenile hormone and sesquiterpenoids in arthropods: Biosynthesis, signaling, and role of MicroRNA
This article provides an overview of microRNAs that are known to regulate sesquiterpenoid production in arthropods. (Source: The Journal of Steroid Biochemistry and Molecular Biology)
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 11, 2018 Category: Biochemistry Source Type: research

Discovery and development of ODM-204: A Novel nonsteroidal compound for the treatment of castration-resistant prostate cancer by blocking the androgen receptor and inhibiting CYP17A1
We report the discovery of a novel nonsteroidal dual-action compound, ODM-204, that holds promise for treating patients with castration-resistant prostate cancer (CRPC), an advanced form of prostate cancer characterised by high androgen receptor (AR) expression and persistent activation of the AR signaling axis by residual tissue androgens. For ODM-204, has a dual mechanism of action. The compound is anticipated to efficiently dampen androgenic stimuli in the body by inhibiting CYP17A1, the prerequisite enzyme for the formation of dihydrotestosterone (DHT) and testosterone (T), and by blocking AR with high affinity and spe...
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 11, 2018 Category: Biochemistry Source Type: research

A possible principal function of corticosteroid signaling that is conserved in vertebrate evolution: Lessons from receptor-knockout small fish
Publication date: Available online 23 February 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Tatsuya Sakamoto, Susumu Hyodo, Wataru TakagiAbstractCorticosteroid receptors are critical for homeostasis maintenance, but understanding of the principal roles of the glucocorticoid receptor (GR) and mineralocorticoid receptor (MR) throughout vertebrates is limited. Lines of constitutive GR-knockout zebrafish and MR-knockout medaka have recently been generated as the first adult-viable corticosteroid receptor-knockout animals, in contrast to the lethality of these receptor knockouts in mice. Here,...
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 11, 2018 Category: Biochemistry Source Type: research

Conservation of DNA and ligand binding properties of retinoid X receptor from the placozoan Trichoplax adhaerens to human
Publication date: Available online 3 March 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Adam M. Reitzel, Jason Macrander, Daniel Mane-Padros, Bin Fang, Frances M. Sladek, Ann M. TarrantAbstractNuclear receptors are a superfamily of transcription factors restricted to animals. These transcription factors regulate a wide variety of genes with diverse roles in cellular homeostasis, development, and physiology. The origin and specificity of ligand binding within lineages of nuclear receptors (e.g., subfamilies) continues to be a focus of investigation geared toward understanding how the funct...
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 11, 2018 Category: Biochemistry Source Type: research

HMGA1a induces alternative splicing of estrogen receptor alpha in MCF-7 human breast cancer cells
Publication date: Available online 17 April 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Kenji Ohe, Shinsuke Miyajima, Ichiro Abe, Tomoko Tanaka, Yuriko Hamaguchi, Yoshihiro Harada, Yuta Horita, Yuki Beppu, Fumiaki Ito, Takafumi Yamasaki, Hiroki Terai, Masayoshi Mori, Yusuke Murata, Makito Tanabe, Kenji Ashida, Kunihisa Kobayashi, Munechika Enjoji, Toshihiko Yanase, Nobuhiro Harada, Toshiaki UtsumiAbstractThe high-mobility group A protein 1a (HMGA1a) protein is known as an oncogene whose expression level in cancer tissue correlates with the malignant potential, and known as a component of...
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 11, 2018 Category: Biochemistry Source Type: research

Polycystic ovary syndrome (PCOS), an inflammatory, systemic, lifestyle endocrinopathy
Publication date: Available online 17 April 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Seema PatelAbstractPolycystic ovary syndrome (PCOS) is an endocrine disorder, afflicting females of reproductive age. This syndrome leads to infertility, insulin resistance, obesity, and cardiovascular problems, including a litany of other health issues. PCOS is a polygenic, polyfactorial, systemic, inflammatory, dysregulated steroid state, autoimmune disease, manifesting largely due to lifestyle errors. The advent of biochemical tests and ultrasound scanning has enabled the detection of PCOS in the a...
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 11, 2018 Category: Biochemistry Source Type: research

Serum 25-hydroxyvitamin D and breast cancer risk by pathological subtype (MCC-Spain)
This study analyzes the association between serum 25-hydroxyvitamin D (25(OH)D) and breast cancer risk by pathologic subtype, stage at diagnosis and specific breast cancer risk factors. We conducted a population-based multicase-control study where 546 histologically-confirmed breast cancer cases and 558 population controls, frequently matched by geographic area, age and body mass index, were recruited in 12 Spanish provinces (MCC-Spain). Information was collected by a questionnaire and plasma 25(OH)D was measured by solid-phase extraction on-line coupled to liquid chromatography–tandem mass spectrometry (SPE–LC...
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 11, 2018 Category: Biochemistry Source Type: research

Vitamin D deficiency promotes prostatic hyperplasia in middle-age mice through exacerbating local inflammation
This study investigated the effects of vitamin D deficiency in early life on prostatic hyperplasia in middle-aged mice. In control group, dams and their male pups were fed with standard-chow diets. In VDD group, dams were fed with vitamin D deficient (VDD) diets throughout pregnancy and lactation. After weaning, male pups continued to be fed with VDD diets. As expected, prostate weight was elevated and prostatic hyperplasia was observed in VDD-fed mice. The number of prostatic Ki-67-positive epithelial cells, a proliferation marker, was increased in VDD-fed mice. Further analysis found that vitamin D deficiency promoted in...
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 11, 2018 Category: Biochemistry Source Type: research

Fetuin B links vitamin D deficiency and pediatric obesity: Direct negative regulation by vitamin D
Publication date: Available online 21 April 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Gillian E. Walker, Antonia Follenzi, Valentina Bruscaggin, Marcello Manfredi, Simonetta Bellone, Emilio Marengo, Luigi Maiuri, Flavia Prodam, Gianni BonaAbstractVitamin D (VD) deficiency (VDD) correlates to obesity, with VD a recognized mediator of metabolic diseases. From a previous proteomic study identifying adiponectin as a link between VDD and pediatric obesity, herein we analysed another protein (SSP2301) increased with VDD. A focused 2D-electrophoretic analysis identified 4 corresponding plasma...
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 11, 2018 Category: Biochemistry Source Type: research

Impairment of the Gnα11-controlled expression of claudin-1 and MMP-9 and collective migration of human breast cancer MCF-7 cells by DHEAS
Publication date: Available online 21 April 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Neha Upmanyu, Ahmed Bulldan, Dimitrios Papadopoulos, Raimund Dietze, Viveka Nand Malviya, Georgios Scheiner-BobisAbstractAlthough dehydroepiandrosterone sulfate (DHEAS) constitutes the most abundant steroid in humans, in-depth investigations of its effects are rather scarce. We address here DHEAS effects on the estrogen receptor-positive metastatic human breast cancer cell line MCF-7. We focus on DHEAS-mediated signaling that might influence expression of claudin-1 and matrix metalloproteinase-9 (MMP-...
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 11, 2018 Category: Biochemistry Source Type: research

A sorafenib-sparing effect in the treatment of thyroid carcinoma cells attained by co-treatment with a novel isoflavone derivative and 1,25 dihydroxyvitamin D3
ConclusionsThe demonstration that low concentrations of cDtboc and 1.25D markedly amplify the inhibitory effect of sorafenib on the growth of human PTC supports the use of a 10-fold lower concentration of sorafenib. The findings may promote a new combination treatment for progressive radioactive iodine-refractory PTC. (Source: The Journal of Steroid Biochemistry and Molecular Biology)
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 11, 2018 Category: Biochemistry Source Type: research

Signaling pathways involved in the rapid biphasic effect of aldosterone on Na+/H+ exchanger in rat proximal tubule cells
Publication date: Available online 24 April 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Deise C.A. Leite-Dellova, Shirley J. Szriber, Giovana K.F. Merighe, Juliano Z. Polidoro, Nancy A. Rebouças, Maria Oliveira-Souza, Margarida de Mello-AiresAbstractThe receptors and signaling pathways for nongenomic effects of aldosterone (Aldo) on the proximal Na+/H+ exchanger are still unknown; therefore, the aim of this study was to investigate the mineralocorticoid receptor (MR) and/or glucocorticoid receptor (GR) participation in rapid Aldo effects on NHE1 (basolateral Na+/H+ exchanger isofo...
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 11, 2018 Category: Biochemistry Source Type: research

Prenatal exposure to benzophenone-3 (BP-3) induces apoptosis, disrupts estrogen receptor expression and alters the epigenetic status of mouse neurons
This study revealed for the first time that prenatal exposure to BP-3 led to apoptosis and neurotoxicity, altered the levels of estrogen receptors (ERs) and changed the epigenetic status of mouse neurons. In the present study, subcutaneous injections of pregnant mice with BP-3 at 50 mg/kg, which is an environmentally relevant dose, evoked activation of caspase-3 and lactate dehydrogenase (LDH) release as well as substantial loss of mitochondrial membrane potential in neocortical cells of their embryonic offspring. Apoptosis-focused microarray analysis of neocortical cells revealed up-regulation of 22 genes involved in ap...
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 11, 2018 Category: Biochemistry Source Type: research

Structure and dynamics of neurosteroid binding to the α1β2γ2 GABAA receptor
Publication date: Available online 26 April 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Lautaro D. Alvarez, Adali PecciAbstractNeurosteroids are the principal endogenous modulators of the γ-Aminobutyric acid receptors (GABAARs), pentameric membrane-bound proteins that can be assembled from at least 19 subunits. In the most abundant GABAAR arrangement (α1β2γ2), neurosteroids can potentiate the GABA action as well as produce a direct activation of the channel. The recent crystal structures of neurosteroids bound to α homopentameric GABAAR reveal binding to five...
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 11, 2018 Category: Biochemistry Source Type: research

Nuclear progestin receptor (Pgr) knockouts resulted in subfertility in male tilapia (Oreochromis niloticus)
Publication date: Available online 27 April 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Xuelian Fang, Limin Wu, Lanying Yang, Lingyun Song, Jing Cai, Feng Luo, Jing Wei, Linyan Zhou, Deshou WangAbstractIt was documented that 17α, 20β-dihydroxy-4-pregnen-3-one (DHP), a fish specific progestin, might play critical roles in spermatogenesis, sperm maturation and spermiation partially through activating nuclear receptor (Pgr). However, no direct evidence is available to demonstrate the functions of DHP in fish spermatogenesis. To further elucidate the roles of DHP in teleosts, we g...
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 11, 2018 Category: Biochemistry Source Type: research

Delineating the regulation of estrogen and androgen receptor expression by sex steroids during rat spermatogenesis
Publication date: Available online 27 April 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Anita Kumar, Kushaan Dumasia, Sharvari Deshpande, Sanketa Raut, N.H. BalasinorAbstractEstrogen receptors (ERα and β) and androgen receptor (AR) regulate various critical processes during spermatogenesis. Since spermatogenesis is very sensitive to hormonal stimuli and perturbations, it is important to understand the regulation of expression of these receptors by sex steroid hormones. Although many studies have reported deregulation of steroid receptors on endocrine disruption, there is no co...
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 11, 2018 Category: Biochemistry Source Type: research

Human-specific features of the G-quadruplex in the androgen receptor gene promoter: A comparative structural and dynamics study
Publication date: Available online 28 April 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Christian Solís-Calero, Taize M. Augusto, Hernandes F. CarvalhoAbstractThe androgen receptor (AR) promoter contains guanine-rich regions that are able to fold into polymorphic G-quadruplex (GQ) structures, and whose deletion decreases AR gene transcription. Our attention was focused on this region because of the frequent termination of sequencing reactions during promoter methylation studies. UV and circular dichroism (CD) spectroscopy of synthetic oligonucleotides encompassing these guanine-ri...
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 11, 2018 Category: Biochemistry Source Type: research

The contribution of cholesterol and epigenetic changes to the pathophysiology of breast cancer
Publication date: Available online 4 May 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Maliha T. Munir, Christopher Ponce, Catherine A. Powell, Kaiser Tarafdar, Teru Yanagita, Mahua Choudhury, Lauren S. Gollahon, Shaikh M. RahmanAbstractBreast cancer is one of the most commonly diagnosed cancers in women. Accumulating evidence suggests that cholesterol plays an important role in the development of breast cancer. Even though the mechanistic link between these two factors is not well understood, one possibility is that dysregulated cholesterol metabolism may affect lipid raft and membrane fl...
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 11, 2018 Category: Biochemistry Source Type: research

Estrogens and selective estrogen receptor modulators differentially antagonize Runx2 in ST2 mesenchymal progenitor cells
Publication date: Available online 8 May 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Yonatan Amzaleg, Jie Ji, Donlaporn Kittivanichkul, Anna E Törnqvist, Sara Windahl, Elias Sabag, Aysha B. Khalid, Hal Sternberg, Michael West, John A. Katzenellenbogen, Susan A. Krum, Nyam-Osor Chimge, Dustin E. Schones, Yankel Gabet, Claes Ohlsson, Baruch FrenkelAbstractEstrogens attenuate bone turnover by inhibiting both osteoclasts and osteoblasts, in part through antagonizing Runx2. Apparently conflicting, stimulatory effects in osteoblast lineage cells, however, sway the balance between bone res...
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 11, 2018 Category: Biochemistry Source Type: research

Basal glucocorticoid receptor activation induces proliferation and inhibits neuronal differentiation of human induced pluripotent stem cell-derived neuronal precursor cells
Publication date: Available online 8 May 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Elina Nürnberg, Sandra Horschitz, Patrick Schloss, Andreas Meyer-LindenbergAbstractGlucocorticoids (GC) have first been shown to originate from the adrenal glands where synthesis and release is controlled by the hypothalamic-pituitary-adrenal (HPA) axis. Recently, it was shown that GC and other steroid hormones are also synthesized in the central nervous system, so-called neurosteroids. GC bind to specific GC receptors (GR) which function as ligand-activated transcription factors. GR are expressed i...
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 11, 2018 Category: Biochemistry Source Type: research

Impact of steroid hormones E2 and P on the NLRP3/ASC/Casp1 axis in primary mouse astroglia and BV-2 cells after in vitro hypoxia
Publication date: Available online 15 May 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Alexander Slowik, Leoni Lammerding, Adib Zendedel, Pardes Habib, Cordian BeyerAbstractClinical and animal model studies have demonstrated the neuroprotective and anti-inflammatory effects of 17beta-estradiol (E2) and progesterone (P) in different disease models of the central nervous system (CNS) including ischemic stroke. Inflammasomes are involved in the interleukin-1 beta (IL1beta) maturation, in particular, NLRP3, the adaptor protein apoptosis-associated speck-like protein containing a CARD (ASC) an...
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 11, 2018 Category: Biochemistry Source Type: research

Dehydroepiandrosterone: Molecular mechanisms and therapeutic implications in osteoarthritis
Publication date: Available online 19 May 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Kai Huang, Li-dong WuAbstractDehydroepiandrosterone (DHEA), a 19-carbon steroid hormone primarily synthesized in the adrenal gland, exerts a chondroprotective effect against osteoarthritis (OA) and has been considered an effective candidate of disease-modifying OA drugs (DMOADs) that slow disease progression. We and others previously demonstrated that DHEA exerted a beneficial effect on osteoarthritic cartilage by positively modulating the balance between anabolic and catabolic factors (e.g., MMPs/TIMP-...
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 11, 2018 Category: Biochemistry Source Type: research

Hormone-dependent breast cancer: Targeting autophagy and PI3K overcomes Exemestane-acquired resistance
Publication date: Available online 20 May 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Cristina Amaral, Tiago Vieira Augusto, Elisiário Tavares-da-Silva, Fernanda M.F. Roleira, Georgina Correia-da-Silva, Natércia TeixeiraAbstractThe leading cause of cancer death in women around the world is breast cancer. The aromatase inhibitors (AIs) are considered - as first-line treatment for estrogen receptor-positive (ER+) breast tumors, in postmenopausal women. Exemestane (Exe) is a powerful steroidal AI, however, despite its therapeutic success, Exe-acquired resistance may occur lead...
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 11, 2018 Category: Biochemistry Source Type: research

Impact of genetic influence on serum total- and free 25-hydroxyvitamin-D in humans
Publication date: Available online 21 May 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Balázs Szili, Boglárka Szabó, Péter Horváth, Bence Bakos, Györgyi Kirschner, János P. Kósa, Erzsébet Toldy, Zsuzsanna Putz, Péter Lakatos, Ádám Tabák, István TakácsAbstractSerum 25-hydroxyvitamin D /25OHD/ levels in humans are determined primarily by environmental factors such as UV-B radiation and diet, including vitamin D intake. Although some genetic determinants of 25OHD levels have been shown, the...
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 11, 2018 Category: Biochemistry Source Type: research

Estradiol dimer inhibits tubulin polymerization and microtubule dynamics
Publication date: Available online 24 May 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Michal Jurášek, Markéta Černohorská, Jiří Řehulka, Vojtěch Spiwok, Tetyana Sulimenko, Eduarda Dráberová, Maria Darmostuk, Soňa Gurská, Ivo Frydrych, Renata Buriánová, Tomáš Ruml, Marián Hajdúch, Petr Bartůněk, Pavel Dráber, Petr Džubák, Pavel B. Drašar, David SedlákAbstractMicrotubule dynamics is one of the major targets for new chemotherapeutic agents. This communication...
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 11, 2018 Category: Biochemistry Source Type: research

Steroid sulfatase inhibition success and limitation in breast cancer clinical assays: an underlying mechanism
This study has clarified sex-hormone control by sulfatase in BC, suggesting that the different roles of estradiol and 5α-dihydrotestosterone can lead to a reduction in the effect of sulfatase inhibition when compared with 17β-HSD7 inhibition. This suggests that combined treatment of sulfatase inhibitors with 17β-HSD inhibitors such as the type7 inhibitor could hold promise for hormone-dependent breast cancer.Graphic abstract (Source: The Journal of Steroid Biochemistry and Molecular Biology)
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 11, 2018 Category: Biochemistry Source Type: research

Response of serum 25(OH)D to Vitamin D and calcium supplementation in school-children from a semi-rural setting in India
The objectives of this study were to: 1) Determine the impact of varying baseline serum 25OHD on increase in vitamin D concentrations after daily supplementation with vitamin D and calcium (1000 IU + 500 mg respectively) for six months in school-children from a semi-rural setting 2) Test the efficacy of daily vitamin D-calcium supplementation on improvement in serum vitamin D concentrations to ≥75 nmol/L. Data collected from 106 subjects (58 boys, 48 girls), aged 6–12 years, included anthropometric measures like height and weight, body composition analysis, three one-day dietary recalls and sunlight exposu...
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 11, 2018 Category: Biochemistry Source Type: research

Periodontal diseases and adverse pregnancy outcomes: Is there a role for vitamin D?
Publication date: June 2018Source: The Journal of Steroid Biochemistry and Molecular Biology, Volume 180Author(s): Anne Marie Uwitonze, Peace Uwambaye, Moses Isyagi, Chrispinus H. Mumena, Alice Hudder, Afrozul Haq, Kamrun Nessa, Mohammed S. RazzaqueAbstractStudies have shown a relationship between maternal periodontal diseases (PDs) and premature delivery. PDs are commonly encountered oral diseases which cause progressive damage to the periodontal ligament and alveolar bones, leading to loss of teeth and oral disabilities. PDs also adversely affect general health by worsening of cardiovascular and metabolic diseases. Moreo...
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 11, 2018 Category: Biochemistry Source Type: research

Impact of vitamin D on pregnancy-related disorders and on offspring outcome
Publication date: June 2018Source: The Journal of Steroid Biochemistry and Molecular Biology, Volume 180Author(s): Karoline von Websky, Ahmed Abdallah Hasan, Christoph Reichetzeder, Oleg Tsuprykov, Berthold HocherAbstractObservational studies from all over the world continue to find high prevalence rates of vitamin D insufficiency and deficiency in many populations, including pregnant women. Beyond its classical function as a regulator of calcium and phosphate metabolism, vitamin D elicits numerous effects in the human body. Current evidence highlights a vital role of vitamin D in mammalian gestation. During pregnancy, ada...
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 11, 2018 Category: Biochemistry Source Type: research

Editorial Board
Publication date: June 2018Source: The Journal of Steroid Biochemistry and Molecular Biology, Volume 180Author(s): (Source: The Journal of Steroid Biochemistry and Molecular Biology)
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 11, 2018 Category: Biochemistry Source Type: research

Highlights from the 6th International Conference on Vitamin D Deficiency, “Nutrition and Human Health”, Abu Dhabi, United Arab Emirates, March 9-10, 2017
Publication date: June 2018Source: The Journal of Steroid Biochemistry and Molecular Biology, Volume 180Author(s): Afrozul Haq, Sunil J. Wimalawansa, Carsten Carlberg (Source: The Journal of Steroid Biochemistry and Molecular Biology)
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 11, 2018 Category: Biochemistry Source Type: research

Calcium and vitamin D in human health: Hype or real?
Publication date: June 2018Source: The Journal of Steroid Biochemistry and Molecular Biology, Volume 180Author(s): Sunil J. Wimalawansa, Mohammed S. Razzaque, Nasser M. Al-DaghriAbstractThe incidence and prevalence of vitamin D deficiency are increasing worldwide. It is estimated that over 50% of the world’s population have low vitamin D (i.e., hypovitaminosis D; serum levels below 30 ng/mL). In vitamin D inadequacy, human physiological systems work inefficiently. In humans, 80% of the vitamin D is synthesized in the presence of ultraviolet rays from sunlight; for the rest, we rely on diet and nowadays, supplements...
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 11, 2018 Category: Biochemistry Source Type: research

Effect of vitamin D supplementation on serum sclerostin levels in chronic kidney disease
Publication date: June 2018Source: The Journal of Steroid Biochemistry and Molecular Biology, Volume 180Author(s): Ashok Kumar Yadav, Vivek Kumar, Debasish Banerjee, Krishan Lal Gupta, Vivekanand JhaAbstractVitamin D deficiency, cardiovascular disease and abnormal bone mineral metabolism are common in chronic kidney disease (CKD). Abnormal bone mineral metabolism has been linked to vascular calcification in CKD. Sclerostin has emerged as an important messenger in cross talk between bone-vascular axis. We analyzed sclerostin in subjects who participated in the randomized, double blind, placebo controlled trial investigating...
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 11, 2018 Category: Biochemistry Source Type: research

Vascular function and cholecalciferol supplementation in CKD: A self-controlled case series
Publication date: June 2018Source: The Journal of Steroid Biochemistry and Molecular Biology, Volume 180Author(s): Vivek Kumar, Ashok Kumar Yadav, Manphool Singhal, Vinod Kumar, Anupam Lal, Debasish Banerjee, Krishan Lal Gupta, Vivekanand JhaAbstractVitamin D deficiency is common and associated with mortality in chronic kidney disease (CKD) patients. Cardiovascular disease (CVD) is the commonest cause of mortality in CKD patients. In a randomized, double blind, placebo controlled trial, we have recently reported favorable effects of vitamin D supplementation on vascular & endothelial function and inflammatory biomarker...
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 11, 2018 Category: Biochemistry Source Type: research

Efficacy of different modes of vitamin D supplementation strategies in Saudi adolescents
This study was designed to investigate the effects of different vitamin D repletion strategies commonly used on serum vitamin D levels of Saudi adolescents. Study design: A 6-month multi-center, controlled, clinical study, involving 34 schools in the central region of Riyadh, Saudi Arabia. Different strategies of vitamin D supplementation were tested (200 ml fortified milk of different brands or vitamin D tablet (1,000IU). Anthropometrics were taken and fasting blood samples withdrawn at baseline and after intervention for the quantification of serum glucose, lipid profile and 25(OH) vitamin D. A significant increase in ...
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 11, 2018 Category: Biochemistry Source Type: research

Efficacy of Vitamin D interventional strategies in saudi children and adults
In conclusion, oral vitamin D supplementation was the most effective strategy in improving vitamin D status in Saudi adults and children than sunlight exposure or consumption of vitamin D-fortified dairy products. (Source: The Journal of Steroid Biochemistry and Molecular Biology)
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 11, 2018 Category: Biochemistry Source Type: research

Vitamin D: Effects on human reproduction, pregnancy, and fetal well-being
Publication date: June 2018Source: The Journal of Steroid Biochemistry and Molecular Biology, Volume 180Author(s): E.L. Heyden, S.J. WimalawansaAbstractPregnancy places exceptional demands on vitamin D and calcium availability; thus, their deficiencies during pregnancy threaten the woman and her fetus. Globally, vitamin D and other micronutrient deficiencies are common during pregnancy, especially in developing countries where pregnant women have less access to nutritional supplements. Vitamin D deficiency has been reported to be as high as 40% among pregnant women. As a pregnancy progresses, the requirements for vitamin D...
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 11, 2018 Category: Biochemistry Source Type: research

In vivo response of the human epigenome to vitamin D: A Proof-of-principle study
Publication date: June 2018Source: The Journal of Steroid Biochemistry and Molecular Biology, Volume 180Author(s): Carsten Carlberg, Sabine Seuter, Tarja Nurmi, Tomi-Pekka Tuomainen, Jyrki K. Virtanen, Antonio NemeAbstractIn vitro cell culture studies showed that the hormonal form of vitamin D3, 1α,25-dihydroxyvitamin D3, significantly (p 
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 11, 2018 Category: Biochemistry Source Type: research

Serum 25-hydroxyvitamin D levels in multiple sclerosis patients from the north of Portugal
Publication date: June 2018Source: The Journal of Steroid Biochemistry and Molecular Biology, Volume 180Author(s): Andreia Bettencourt, Daniela Boleixa, Henrique Reguengo, Raquel Samões, Ernestina Santos, José Carlos Oliveira, Berta Silva, Paulo Pinho Costa, Ana Martins da SilvaAbstractIncreasing evidence has shown that individuals with Multiple Sclerosis (MS) have lower 25-hydroxyvitamin D [25(OH)D] levels compared to healthy controls. There is no information regarding 25(OH)D levels and MS in Portugal. Therefore the aim of the current study was to examine the levels of 25(OH)D in a group of patients with MS...
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 11, 2018 Category: Biochemistry Source Type: research

Sex-specific expression of apolipoprotein levels following replenishment of vitamin D
Publication date: June 2018Source: The Journal of Steroid Biochemistry and Molecular Biology, Volume 180Author(s): Nasser M. Al-Daghri, Kaiser Wani, Shaun Sabico, Spiro D. Garbis, George P. Chrousos, Osama E. Amer, Mohammed Ghouse Ahmed Ansari, Yousef Al-Saleh, Naji J. Aljohani, Omar S. Al-Attas, Majed S. AlokailAbstractNumerous studies have been done to establish the relationship between vitamin D and lipids, yet a definitive causal link is not found. This interventional study aims to evaluate and compare levels of apolipoproteins among vitamin D deficient subjects at baseline and after they achieved full vitamin D status...
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 11, 2018 Category: Biochemistry Source Type: research