The key action of estradiol and progesterone enables GnRH delivery during gestation in the South American plains vizcacha, Lagostomus maximus
Publication date: Available online 15 February 2020Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Pablo I.F. Inserra, Santiago E. Charif, Victoria Fidel, Mariela Giacchino, Alejandro R. Schmidt, Federico M. Villarreal, Sofía Proietto, Santiago A. Cortasa, María C. Corso, María C. Gariboldi, Noelia P. Leopardo, Nicolás A. Fraunhoffer, Noelia P. Di Giorgio, Victoria A. Lux-Lantos, Julia Halperin, Alfredo D. Vitullo, Verónica B. DorfmanAbstractThe South American plains vizcacha, Lagostomus maximus, is the only mammal described so far that shows expression of estr...
Source: The Journal of Steroid Biochemistry and Molecular Biology - February 16, 2020 Category: Biochemistry Source Type: research

Lipoid Congenital Adrenal Hyperplasia due to Steroid Acute Regulatory Protein (STAR) Variants in Three Chinese Patients
Publication date: Available online 14 February 2020Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Hong Chen, Qianru Zhang, Ruimin Chen, Xin Yuan, Xiangquan Lin, Xiaohong Yang, Ying ZhangAbstractCongenital adrenal hyperplasia (CAH) encompasses a group of autosomal recessive diseases characterized by enzymatic defects in steroid synthesis. Lipoid congenital adrenal hyperplasia (LCAH) is the most severe form of CAH, insofar as the initial step of steroid synthesis is impaired. Variants in the steroid acute regulatory (STAR) gene are responsible for LCAH. To describe the clinical and genetic charac...
Source: The Journal of Steroid Biochemistry and Molecular Biology - February 15, 2020 Category: Biochemistry Source Type: research

Serum Vitamin D Level is Associated with Speed of Processing in Multiple Sclerosis Patients
Publication date: Available online 12 February 2020Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Hala Darwish, Natali Farran, Salem Hannoun, Natalie Tadros, Bassem Yamout, Nabil K. El Ayoubi, Samia J. KhouryAbstractMultiple Sclerosis (MS) is often associated with low serum 25(OH)D levels, as well as cognitive dysfunctions. The relationship between 25(OH)D and the most commonly affected cognitive domain in MS; processing speed, is poorly explored. The purpose of this study is to: (1) assess the effect of serum 25(OH)D change on processing speed in MS, and (2) explore the relationship between se...
Source: The Journal of Steroid Biochemistry and Molecular Biology - February 13, 2020 Category: Biochemistry Source Type: research

Depression-like behaviors are accompanied by disrupted mitochondrial energy metabolism in chronic corticosterone-induced mice
Publication date: Available online 8 February 2020Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Xiaoxian Xie, Qichen Shen, Chunan Yu, Qingfeng Xiao, Jiafeng Zhou, Ze Xiong, Zezhi Li, Zhengwei FuAbstractStress exerts its negative effects by interference with mitochondrial energy production in rodents, and is able to impair mitochondrial bioenergetics. However, the underlying mechanism that stress hormone impacts depression-like behaviors and mitochondrial energy metabolism is still not well understood. Here, we investigated the changes of depression-like behaviors and mitochondrial energy metab...
Source: The Journal of Steroid Biochemistry and Molecular Biology - February 10, 2020 Category: Biochemistry Source Type: research

CYP17A1 exhibits 17αhydroxylase/17,20-lyase activity towards 11β-hydroxyprogesterone and 11-ketoprogesterone metabolites in the C11-oxy backdoor pathway
In this study the 17α-hydroxylase and 17,20-lyase activity of CYP17A1 towards the unconventional C11-oxy C21 steroid substrates and their 5α- and 3α,5α-reduced metabolites was investigated in transfected HEK-293 cells. CYP17A1 catalysed the 17α-hydroxylation of 11OHP4 to 21dF and 11-ketoprogesterone (11KP4) to 21-deoxycortisone (21dE) with negligible hydroxylation of their 5α-reduced metabolites while no lyase activity was detected. The 3α,5α-reduced C11-oxy C21 steroids—5α-pregnan-3α,11β-diol-20-one (3,11diOH-DHP4) and 5α-pregnan-3α-ol-11,2...
Source: The Journal of Steroid Biochemistry and Molecular Biology - February 8, 2020 Category: Biochemistry Source Type: research

Lower total 25-hydroxyvitamin D but no difference in calculated or measured free 25-hydroxyvitamin D serum levels in patients with primary hyperparathyroidism
Publication date: Available online 3 February 2020Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Lingqiong Meng, Chi Su, Sue A Shapses, Aseel Al-Dayyeni, Yuling He, Xiangbing WangAbstractTo evaluate the measured free 25-hydroxyvitamin D [25(OH)D] levels in patients with hyperparathyroidism (PHPT) and healthy controls. Eighty patients with PHPT(n = 40) and age and BMI matched controls (n = 40) were examined. Serum levels of total or free 25(OH)D, vitamin D binding protein (DBP), intact parathyroid hormone (iPTH) and calcium were measured. There was no significant diff...
Source: The Journal of Steroid Biochemistry and Molecular Biology - February 4, 2020 Category: Biochemistry Source Type: research

Synthesis and anti–tumour, immunomodulating activity of diosgenin and tigogenin conjugates
Publication date: Available online 1 February 2020Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): O. Michalak, P. Krzeczyński, M. Cieślak, P. Cmoch, M. Cybulski, K. Królewska-Golińska, J. Kaźmierczak-Barańska, B. Trzaskowski, K. OstrowskaAbstractA series of novel diosgenin (DSG) and tigogenin (TGG) derivatives with diosgenin or tigogenin steroid aglycons linked to levulinic and 3,4–dihydroxycinnamic acids, dipeptides and various amino acids by an ester bond at the C3–oxygen atom of the steroid skeleton has been synthesized. Diosgenyl esters have been prepared by an esteri...
Source: The Journal of Steroid Biochemistry and Molecular Biology - February 2, 2020 Category: Biochemistry Source Type: research

Selective LC-MRM/SIM-MS based profiling of adrenal steroids reveals metabolic signatures of 17α-hydroxylase deficiency
Publication date: Available online 31 January 2020Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Chaelin Lee, Jung Hee Kim, Sun Joon Moon, Jaeyoon Shim, Hugh I. Kim, Man Ho ChoiABSTRACTAdrenal steroids are generated in the adrenal cortex and metabolized by various enzymes such as hydroxylases, dehydrogenases, and reductases. Determining the comprehensive metabolic signatures of adrenal steroids can provide insight into their metabolic functions and roles in the pathophysiology of adrenal diseases, including Cushing’s syndrome (CS) and congenital adrenal hyperplasia (CAH). To this end, we ...
Source: The Journal of Steroid Biochemistry and Molecular Biology - February 1, 2020 Category: Biochemistry Source Type: research

Maternal Vitamin D Deficiency Induces Transcriptomic Changes in Newborn Rat Lungs
Publication date: Available online 30 January 2020Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Erica Mandell, Sharon Ryan, Gregory J. Seedorf, Tania Gonzalez, Steven H. Abman, James C. FleetAbstractVitamin D deficiency (VDD) during pregnancy is common and related to several maternal and fetal morbidities. Vitamin D (VD) plays a role in normal lung development and VDD causes abnormal airway, alveolar, and vascular growth in newborn rats. Here we use an unbiased transcriptomic approach to identify pathways altered in the lungs of offspring from VDD dams. The lungs of newborn offspring from VD r...
Source: The Journal of Steroid Biochemistry and Molecular Biology - February 1, 2020 Category: Biochemistry Source Type: research

Gestational hypercalcemia: prevalence and biochemical profile
In conclusion, most common causes of hypercalcemia were excluded in the majority of women. Hypercalcemic women had a relatively high serum 1,25(OH)2D concentration despite an appropriately suppressed PTH, suggestive of abnormal gestational adaptions. (Source: The Journal of Steroid Biochemistry and Molecular Biology)
Source: The Journal of Steroid Biochemistry and Molecular Biology - February 1, 2020 Category: Biochemistry Source Type: research

Circulating 25-hydroxyvitamin D concentration and cause-specific mortality in the Melbourne Collaborative Cohort Study
Publication date: Available online 30 January 2020Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Alicia K Heath, Allison M Hodge, Peter R Ebeling, David Kvaskoff, Darryl W Eyles, Graham G Giles, Dallas R English, Elizabeth J WilliamsonAbstractVitamin D deficiency is associated with higher all-cause mortality, but associations with specific causes of death are unclear. We investigated the association between circulating 25-hydroxyvitamin D (25(OH)D) concentration and cause-specific mortality using a case-cohort study within the Melbourne Collaborative Cohort Study (MCCS). Eligibility for the cas...
Source: The Journal of Steroid Biochemistry and Molecular Biology - February 1, 2020 Category: Biochemistry Source Type: research

CYP17A1 exhibits 17α-hydroxylase/17,20-lyase activity towards 11β-hydroxyprogesterone and 11-ketoprogesterone metabolites in the C11-oxy backdoor pathway
In this study the 17α-hydroxylase and 17,20-lyase activity of CYP17A1 towards the unconventional C11-oxy C21 steroid substrates and their 5α- and 3α,5α-reduced metabolites was investigated in transfected HEK-293 cells. CYP17A1 catalysed the 17α-hydroxylation of 11OHP4 to 21 dF and 11-ketoprogesterone (11KP4) to 21-deoxycortisone (21dE) with negligible hydroxylation of their 5α-reduced metabolites while no lyase activity was detected. The 3α,5α-reduced C11-oxy C21 steroids —5α-pregnan-3α,11β-diol-20-one (3,11diOH-DHP4) and 5α-pregnan-3&alpha...
Source: The Journal of Steroid Biochemistry and Molecular Biology - February 1, 2020 Category: Biochemistry Source Type: research

In memoriam: AW Norman, the founding father of the Vitamin D Workshops
Publication date: Available online 30 January 2020Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Roger KU Leuven (Source: The Journal of Steroid Biochemistry and Molecular Biology)
Source: The Journal of Steroid Biochemistry and Molecular Biology - January 30, 2020 Category: Biochemistry Source Type: research

Unveiling molecular associations of polymorphic variants of VDR gene (FokI, BsmI and ApaI) in multiple myeloma patients of Indian population
In conclusion, this study provided initial evidences of association between 25(OH)D insufficiency, VDR gene polymorphism and MM development. Thus, we suggest that a study involving assessment of 25(OH)D levels and VDR gene polymorphism in large patients’ cohort might substantiate their role in MM development which would further provide impetus to give 25(OH)D supplementation along with conventional chemotherapeutic agents for myeloma treatment in future. (Source: The Journal of Steroid Biochemistry and Molecular Biology)
Source: The Journal of Steroid Biochemistry and Molecular Biology - January 30, 2020 Category: Biochemistry Source Type: research

Fibroblast growth factor 23 counters vitamin D metabolism and action in human mesenchymal stem cells
In this study, we tested the hypothesis that FGF23 inhibits vitamin D metabolism and action in hMSCs. hMSCs were isolated from discarded marrow during hip arthroplasty, including two subjects receiving hemodialysis and a series of 20 subjects (aged 49-83 years) with estimated glomerular filtration rate (eGFR) data. The direct in vitro effects of rhFGF23 on hMSCs were analyzed by RT-PCR, Western immunoblot, and biochemical assays. Ex vivo analyses showed positive correlations for both secreted and membrane-bound αKlotho gene expression in hMSCs with eGFR of the subjects from whom hMSCs were isolated. There was a downr...
Source: The Journal of Steroid Biochemistry and Molecular Biology - January 30, 2020 Category: Biochemistry Source Type: research

Prevalence and factors associated with hypovitaminosis D in adolescents from a sunny country: findings from the ERICA survey
In conclusion, there is a high prevalence of Brazilian adolescents at risk of hypovitaminosis D, independent of region. Due to their potential benefits, lifestyle changes should be stimulated, including healthier food choices and spending more time outdoors (with sun protection). (Source: The Journal of Steroid Biochemistry and Molecular Biology)
Source: The Journal of Steroid Biochemistry and Molecular Biology - January 30, 2020 Category: Biochemistry Source Type: research

In silico identification of novel transcription factors associated with CYP27B1 transcriptional regulation in LPS-challenged mononuclear phagocytes
Publication date: Available online 27 January 2020Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Romina P. Martinelli, Julia M. Rodriguez, Lucas D. Daurelio, Luis EstebanAbstractRenal and extrarenal production of the active form of vitamin D, 1,25-dihydroxyvitamin D (1,25(OH)2D), is catalyzed by CYP27B1, an enzyme also called 1-α-hydroxylase. The overproduction of 1,25(OH)2D has been described in granulomatous diseases. High circulating concentrations of 1,25(OH)2D can lead to hypercalcemia. The aim of this work was to characterize the transcriptional regulation of CYP27B1 in human mononu...
Source: The Journal of Steroid Biochemistry and Molecular Biology - January 29, 2020 Category: Biochemistry Source Type: research

Vitamin D intake and the 10-year risk of urgency urinary incontinence in women
The objective was to assess whether greater vitamin D intake was associated with decreased risk of incident urgency and mixed UI over 10 years using 2 large prospective cohorts of middle-aged and older women. We analyzed 38,101 women from the Nurses’ Health Study I (NHS I) and 35,190 women from NHS II who were free of UI at baseline. We followed incident UI, defined as new UI occurring at least monthly, separately by subtype (urgency, mixed, stress UI), from 2002-2012. We categorized vitamin D intake from supplements and diet. We estimated relative risk for developing UI according to vitamin D intake using Cox-propor...
Source: The Journal of Steroid Biochemistry and Molecular Biology - January 29, 2020 Category: Biochemistry Source Type: research

miR-326 down-regulate CYP19A1 expression and estradiol-17b production in buffalo granulosa cells through CREB and C/EBP-β
Publication date: Available online 26 January 2020Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Vaishali Chaurasiya, Suman Kumari, Suneel Kumar Onteru, Dheer SinghAbstractOvarian granulosa cells, known to be endocrine cells, have well active TLR4-/NFKB signalling mediated innate immune capabilities. We have previously shown that endotoxin not only transiently regulates proinflammatory cytokines but cells become tolerant on repeated exposure to endotoxin and impaired granulosa cells functions, which includes downregulation of CYP19A1 gene. To understand further endotoxin tolerance and impaired ...
Source: The Journal of Steroid Biochemistry and Molecular Biology - January 27, 2020 Category: Biochemistry Source Type: research

Bifidobacterium pseudolongum reduces triglycerides by modulating gut microbiota in mice fed high-fat food
Publication date: Available online 24 January 2020Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Tingbei Bo, Jing Wen, Yuanchun Zhao, Shuangjie Tian, Xueying Zhang, Dehua WangAbstractObesity has become a growing concern around the world. The purpose of this study was to investigate the potential benefit of Bifidobacterium pseudolongum (B. pseudolongum) on obesity, gut microbiota, and its physiological mechanism. The obese mice model was established with a high-fat diet (HFD), and the treatment were used the strain B. pseudolongum. We investigated the changes in fat content, plasma metabolites a...
Source: The Journal of Steroid Biochemistry and Molecular Biology - January 25, 2020 Category: Biochemistry Source Type: research

Systemic distribution of progesterone receptor subtypes in human tissues
In this study, we immunolocalized PR expression in various human tissues using PR (1294) specific antibody, which is capable of detecting both PRA and PRB, and PRB (250H11) specific antibody. Tissues from the uterus, ovary, breast, placenta, prostate, testis, cerebrum, cerebellum, pituitary, spinal cord, esophagus, stomach, small intestine, colon, pancreas, liver, kidney, urinary bladder, lung, heart, aorta, thymus, adrenal gland, thyroid, spleen, skin, and bone were examined in four different age groups (fetal, pediatric, young, and old) in male and female subjects. PR and PRB were detected in the nuclei of cells in the f...
Source: The Journal of Steroid Biochemistry and Molecular Biology - January 25, 2020 Category: Biochemistry Source Type: research

Canadian Recommendations for Vitamin D Intake for Persons Affected by Multiple Sclerosis
Publication date: Available online 22 January 2020Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Stephanie A. Atkinson, James C. FleetAbstractIn 2016, the Multiple Sclerosis (MS) Society of Canada convened a panel of expert scientists, clinicians and patient advocate to review the evidence for an association between vitamin D status and MS prevention and/or disease modification. The goal was to develop clear and accurate recommendations on optimal vitamin D intake and status for people affected by MS for use in clinical practice and public health policy. The final consensus report was based on ...
Source: The Journal of Steroid Biochemistry and Molecular Biology - January 23, 2020 Category: Biochemistry Source Type: research

Impact of Dietary Vitamin D on Initiation and Progression of Oral Cancer
Publication date: Available online 22 January 2020Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Aparajita Verma, Vui King Vincent-Chong, Hendrik DeJong, Pamela A. Hershberger, Mukund SeshadriABSTRACTCalcitriol, the active metabolite of vitamin D, has been widely studied for its preventive and therapeutic activity against several cancers including oral squamous cell carcinoma (OSCC). However, the impact of dietary vitamin D supplementation on initiation and progression of OSCC is unclear. To address this gap in knowledge, we conducted preclinical trials using the 4-nitroquinoline-1-oxide (4NQO)...
Source: The Journal of Steroid Biochemistry and Molecular Biology - January 23, 2020 Category: Biochemistry Source Type: research

Molecular mechanisms of posaconazole- and itraconazole-induced pseudohyperaldosteronism and assessment of other systemically used azole antifungals
Publication date: Available online 23 January 2020Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Katharina R. Beck, Lucija Telisman, Chris J. van Koppen, George R. Thompson, Alex OdermattAbstractRecent reports described cases of severe hypertension and hypokalemia accompanied by low renin and aldosterone levels during antifungal therapy with posaconazole and itraconazole. These conditions represent characteristics of secondary endocrine hypertension caused by mineralocorticoid excess. Different mechanisms can cause mineralocorticoid excess, including inhibition of the adrenal steroidogenic enzy...
Source: The Journal of Steroid Biochemistry and Molecular Biology - January 23, 2020 Category: Biochemistry Source Type: research

Molecular docking, anti-proliferative activity and induction of apoptosis in human liver cancer cells treated with androstane derivatives: Implication of PI3K/AKT/mTOR pathway
Publication date: Available online 23 January 2020Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Shahad W. Kattan, Mohamed S. Nafie, Gamal A. Elmgeed, Walla Alelwani, Muhammad Badar, Mohamed A. TantawyAbstractWorldwide, cancer still an area with high unmet medical need. Lead optimization efforts toward structure-based drug design were employed to discover newly synthesized hetero-steroid derivatives with promising anticancer effects against hepatocellular carcinoma (HCC). The aim of our study is to evaluate the anti-proliferative activity, a dual PI3K/mTOR inhibitor, and mechanism of action of ...
Source: The Journal of Steroid Biochemistry and Molecular Biology - January 23, 2020 Category: Biochemistry Source Type: research

Development and application of a LC–MS/MS assay for simultaneous analysis of 25-hydroxyvitamin-D and 3-epi-25-hydroxyvitamin-D metabolites in canine serum
We describe a simplified workflow utilising supported liquid extraction (SLE) without derivatization that provides good linearity (mean r> 0.996) and accuracy across a broad dynamic range of 4–500 nmol/L for D3 metabolites and 7.8–500 nmol/L for D2 metabolites. Upon application of this assay to 117 canine serum samples, 25(OH)D3 was detectable in all samples with a median concentration of 82.1 nmol/L (inter-quartile range (IQR) 59.7–101.8 nmol/L). 3-epi-25(OH)D3 could be detected in 87.2 % of the study population, with a median concentration of 5.2 nmol/L (2.4–8.1 nmol/L). However, 3-...
Source: The Journal of Steroid Biochemistry and Molecular Biology - January 23, 2020 Category: Biochemistry Source Type: research

Expression of estrogen receptor α variants and c-Fos in rat mammary gland and tumors
Publication date: Available online 20 January 2020Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Alicia Gutiérrez, Lorena Sambuco, Laura Álvarez, Mariel Núñez, Rosa Bergoc, Elsa Zotta, Gabriela Martín, Andrea RandiAbstractBreast cancer is currently the leading cause of cancer death among women worldwide. AP-1 (c-Fos/c-Jun) is associated with proliferation and survival, while cytoplasmic c-Fos activates phospholipid synthesis in cells induced to differentiate or grow. Estrogen receptor α 46 (ERα46) is a splice variant of full-length ERα66 and ...
Source: The Journal of Steroid Biochemistry and Molecular Biology - January 21, 2020 Category: Biochemistry Source Type: research

Characteristics and sex dimorphism of 17β-hydroxysteroid dehydrogenase family genes in the olive flounder Paralichthys olivaceus
In this study, a total of ten Hsd17b family genes, including Hsd17b3, -4, -7, -8, -9, -10, -12a, -12b, -14 and -15, were identified in the flounder, which encoded critical enzymes acting on sex steroid synthesis and metabolism. Hsd17b genes were distributed on eight chromosomes. Hsd17b12a and -12b were located on chromosomes 19 and 7, respectively. It was speculated that these two genes were just highly similar rather than different transcripts derived from the same gene. According to the results of domain and motif analyses, they all belonged to the SDR superfamily and contained conserved Hsd17b motifs TGxxxGxG, PGxxxT, N...
Source: The Journal of Steroid Biochemistry and Molecular Biology - January 18, 2020 Category: Biochemistry Source Type: research

Daily and seasonal mitochondrial protection: unraveling common possible mechanisms involving vitamin d and melatonin
Publication date: Available online 16 January 2020Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Feres José Mocayar Marón, León Ferder, Russel J. Reiter, Walter ManuchaAbstractFrom an evolutionary point of view, vitamin D and melatonin appeared very early and share functions related to defense mechanisms. In the current clinical setting, vitamin D is exclusively associated with phosphocalcic metabolism. Meanwhile, melatonin has chronobiological effects and influences the sleep-wake cycle. Scientific evidence, however, has identified new actions of both molecules in differen...
Source: The Journal of Steroid Biochemistry and Molecular Biology - January 17, 2020 Category: Biochemistry Source Type: research

Sex dimorphism in an animal model of multiple sclerosis: focus on pregnenolone synthesis
Publication date: Available online 17 January 2020Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): S. Giatti, R. Rigolio, S. Diviccaro, E. Falvo, D. Caruso, L.M. Garcia-Segura, G. Cavaletti, R.C. MelcangiABSTRACTNeuroactive steroids, molecules produced from cholesterol in steroidogenic cells (i.e., peripheral glands and nervous system) are physiological modulators and protective agents of nervous function. A possible role for neuroactive steroids in the sex-dimorphic clinical manifestation, onset and progression of Multiple Sclerosis (MS) has been recently suggested. To explore this possibility, ...
Source: The Journal of Steroid Biochemistry and Molecular Biology - January 17, 2020 Category: Biochemistry Source Type: research

VDR in Salivary Gland Homeostasis and Cancer
Publication date: Available online 17 January 2020Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Kara A. DeSantis, Samantha L. Robilotto, Mark Matson, Noor M. Kotb, Cathryn M. Lapierre, Zenab Minhas, Alana A. Leder, Khushbakht Abdul, Emily M. Facteau, JoEllen WelshAbstractThe vitamin D receptor (VDR) and its ligand 1,25(OH)2D3 (1,25D) impact differentiation and exert anti-tumor effects in many tissues, but its role in salivary gland has yet to be defined. Using immunohistochemistry (IHC), we have detected strong VDR expression in murine and human salivary gland ducts. Compared to normal gland, ...
Source: The Journal of Steroid Biochemistry and Molecular Biology - January 17, 2020 Category: Biochemistry Source Type: research

Development and application of a LC-MS/MS assay for simultaneous analysis of 25-hydroxyvitamin-D and 3-epi-25-hydroxyvitamin-D metabolites in canine serum
We describe a simplified workflow utilising supported liquid extraction (SLE) without derivatization that provides good linearity (mean r> 0.996) and accuracy across a broad dynamic range of 4 - 500 nmol/L for D3 metabolites and 7.8 – 500 nmol/L for D2 metabolites. Upon application of this assay to 117 canine serum samples, 25(OH)D3 was detectable in all samples with a median concentration of 82.1 nmol/L (inter-quartile range (IQR) 59.7 – 101.8 nmol/L). 3-epi-25(OH)D3 could be detected in 87.2% of the study population, with a median concentration of 5.2 nmol/L (2.4 – ...
Source: The Journal of Steroid Biochemistry and Molecular Biology - January 17, 2020 Category: Biochemistry Source Type: research

Impact of Maternal Smoking Associated Lyso-phosphatidylcholine 20:3 on Offspring Brain Development
Publication date: Available online 15 January 2020Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Lu Yong-Ping, Christoph Reichetzeder, Cornelia Prehn, Liang-Hong Yin, Chang Chu, Saban Elitok, Bernhard K. Krämer, Jerzy Adamski, Berthold HocherAbstractMaternal smoking during pregnancy affects fetal neurological development. Metabolomic studies in the general population suggest that smoking is associated with characteristic metabolic alterations. We investigated the association between the maternal smoking status, the fetal metabolome and head circumference at birth, as a surrogate parameter ...
Source: The Journal of Steroid Biochemistry and Molecular Biology - January 16, 2020 Category: Biochemistry Source Type: research

Synthesis and application of steroidal 22,16β-carbolactones: a review
Publication date: Available online 14 January 2020Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Izabella JastrzebskaGraphical abstract (Source: The Journal of Steroid Biochemistry and Molecular Biology)
Source: The Journal of Steroid Biochemistry and Molecular Biology - January 15, 2020 Category: Biochemistry Source Type: research

Vitamin D and sex steroid production in men with normal or impaired Leydig cell function
Publication date: Available online 15 January 2020Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Rune Holt, Li Juel Mortensen, Katrine Harpelunde Poulsen, John Erik Nielsen, Hanne Frederiksen, Niels Jørgensen, Anne Jørgensen, Anders Juul, Martin Blomberg JensenAbstractProduction of testosterone is under tight control by human chorion gonadotropin (hCG) during fetal life and luteinizing hormone (LH) in adulthood. Several animal and human studies have linked vitamin D status with sex steroid production although it is not clear whether there exist a direct or indirect involvement in ...
Source: The Journal of Steroid Biochemistry and Molecular Biology - January 15, 2020 Category: Biochemistry Source Type: research

25(OH)D3 stimulates the expression of vitamin D target genes in renal tubular cells when Cyp27b1 is abrogated
Publication date: Available online 13 January 2020Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Takahiro Kikuyama, Takao Susa, Mimi Tamamori-Adachi, Masayoshi Iizuka, Miho Akimoto, Hiroko Okinaga, Yoshihide Fujigaki, Shunya Uchida, Shigeru Shibata, Tomoki OkazakiAbstractRecently, it was reported that 25(OH)D3 (25D3) has physiological bioactivity in certain tissues derived from Cyp27b1 knockout mice. To investigate the function of 25D3 in the kidney as an informational crossroad of various calciotropic substances, we employed the CRISPR-Cas9 system to knock out Cyp27b1 in the mouse renal distal...
Source: The Journal of Steroid Biochemistry and Molecular Biology - January 14, 2020 Category: Biochemistry Source Type: research

Cholestenoic Acid Analogues as Inverse Agonists of the Liver X Receptors
Publication date: Available online 10 January 2020Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Lautaro D. Alvarez, María V. Dansey, María F. Ogara, Carina I. Peña, René Houtman, Adriana S. Veleiro, Adali Pecci, Gerardo BurtonAbstractLiver X Receptors (LXRs) are ligand dependent transcription factors activated by oxidized cholesterol metabolites (oxysterols) that play fundamental roles in the transcriptional control of lipid metabolism, cholesterol transport and modulation of inflammatory responses. In the last decade, LXRs have become attractive pharmacological tar...
Source: The Journal of Steroid Biochemistry and Molecular Biology - January 11, 2020 Category: Biochemistry Source Type: research

Human dehydrogenase/reductase SDR family member 11 (DHRS11) and aldo-keto reductase 1C isoforms in comparison: Substrate and reaction specificity in the reduction of 11-keto-C19-steroids
Publication date: Available online 8 January 2020Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Satoshi Endo, Yoshifumi Morikawa, Yudai Kudo, Koichi Suenami, Toshiyuki Matsunaga, Akira Ikari, Akira HaraAbstractRecent studies have shown that an adrenal steroid 11β-hydroxy-4-androstene-3,17-dione serves as the precursor to androgens, 11-ketotestosterone and 11-ketodihydrotestosterone (11KDHT). The biosynthetic pathways include the reduction of 3- and 17-keto groups of the androgen precursors 11-keto-C19-steroids, which has been reported to be mediated by three human enzymes; aldo-keto reduct...
Source: The Journal of Steroid Biochemistry and Molecular Biology - January 9, 2020 Category: Biochemistry Source Type: research

Chemotherapy and vitamin D supplement use are determinants of serum 25-hydroxyvitamin D levels during the first six months after colorectal cancer diagnosis
In conclusion, vitamin D supplement use and treatment appear to be important determinants of 25(OH)D3 levels during the first six months after CRC diagnosis, although the difference in 25(OH)D3 levels was minor.ClinicalTrials.gov Identifier: NCT03191110 (Source: The Journal of Steroid Biochemistry and Molecular Biology)
Source: The Journal of Steroid Biochemistry and Molecular Biology - January 8, 2020 Category: Biochemistry Source Type: research

Investigating a suitable model for the study of vitamin D mediated regulation of human placental gene expression
This study compares the expression of amino acid transporters and vitamin D related genes in human term placenta with the BeWo and human embryonic kidney (HEK)293 cell lines. HEK293 cells, as transporting epithelium may be more similar to placenta. Gene expression in term placenta was much more similar to HEK293 than BeWo. This study provides further evidence that the BeWo cell line is not an appropriate model for villous trophoblast and a model that more closely represents the human placenta is now required to investigate the effects of vitamin D on the placenta ex-vivo. (Source: The Journal of Steroid Biochemistry and Molecular Biology)
Source: The Journal of Steroid Biochemistry and Molecular Biology - January 3, 2020 Category: Biochemistry Source Type: research

The role of diosgenin in diabetes and diabetic complications
This article reviews the effects of diosgenin through multiple targets and multiple pathways in diabetes and its complications which including diabetic nephropathy, diabetic liver disease, diabetic neuropathy, diabetic vascular disease, diabetic cardiomyopathy, diabetic reproductive dysfunction, and diabetic eye disease. (Source: The Journal of Steroid Biochemistry and Molecular Biology)
Source: The Journal of Steroid Biochemistry and Molecular Biology - December 31, 2019 Category: Biochemistry Source Type: research

Association of nonalcoholic fatty liver disease with serum vitamin D levels in combination of physical fitness in Korean older adults
Publication date: Available online 28 December 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Inhwan Lee, Eunmi Park, Jinkyung ChoAbstractThe prevalence of non-alcoholic fatty liver disease (NAFLD) tends to increase with age, but little is known regarding relations between the risk of NAFLD in older adults and serum vitamin D (25-hydroxyvitamin D) and physical fitness levels. The aim of this study was to investigate the relationship between NAFLD risk and serum vitamin D levels combined with physical fitness in elderly adults using a non-invasive diagnostic approach. We enrolled 533 Korean ...
Source: The Journal of Steroid Biochemistry and Molecular Biology - December 30, 2019 Category: Biochemistry Source Type: research

The implications of vitamin content in the plasma in reference to the parameters of carbohydrate metabolism and hormone and lipid profiles in PCOS
Publication date: Available online 26 December 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Małgorzata Szczuko, Viktoria Hawryłkowicz, Justyna Kikut, Arleta DrozdAbstractSo far, there have been no analyses of correlations between the level of water-soluble vitamins in women with polycystic ovary syndrome (PCOS) and hormone and lipid profiles as well as carbohydrate metabolism. The unpopular concept that PCOS may also be conditioned by a chronic infection leads to a suspicion that water-soluble vitamins may be involved in the struggle against PCOS. This is why the aim of this research wa...
Source: The Journal of Steroid Biochemistry and Molecular Biology - December 27, 2019 Category: Biochemistry Source Type: research

Structure-dependent retention of steroid hormones by common laboratory materials
Publication date: Available online 26 December 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Jeffrey M. McManus, Nima SharifiAbstractThe tendency of steroid molecules to adsorb to various materials, particularly plastics, has been known of for decades but has not received widespread attention in the scientific community, and a modern, systematic study is lacking. This adsorption is an important consideration for researchers working with steroid hormones as it could skew the results of various experiments. Here we show that steroids adsorb to various vessels used in experiments, including m...
Source: The Journal of Steroid Biochemistry and Molecular Biology - December 27, 2019 Category: Biochemistry Source Type: research

Ordered structure-forming properties of the intrinsically disordered AB region of hRXRγ and its ability to promote liquid-liquid phase separation
Publication date: Available online 24 December 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Katarzyna Sołtys, Andrzej OżyharAbstractThe retinoid X receptor (RXR) is a member of the nuclear receptor (NR) superfamily that occupies the central position among other NRs by forming both homodimers and heterodimers with other representatives of the family. RXR shares similar structural domains with other members of NRs. The major differences in the subtypes and isoforms of RXR are in the AB region. To date, there have been no data concerning the molecular properties of the AB region of hRXR&ga...
Source: The Journal of Steroid Biochemistry and Molecular Biology - December 26, 2019 Category: Biochemistry Source Type: research

Targeting 1,25(OH)2D-Mediated Calcium Absorption Machinery in Proximal Colon with Calcitriol Glycosides and Glucuronides
Publication date: Available online 24 December 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): H. Jiang, R.L. Horst, N.J. Koszewski, J.P. Goff, S. Christakos, J.C. FleetAbstractHigh intestinal calcium (Ca) absorption efficiency is associated with high peak bone mass in adolescents and reduced bone loss in adulthood. Transepithelial intestinal Ca absorption is mediated by 1,25-dihydroxyvitamin D (1,25(OH)2D, calcitriol) through the vitamin D receptor (VDR). Most research on Ca absorption focuses on the proximal small intestine but evidence shows that large intestine plays a crucial role in wh...
Source: The Journal of Steroid Biochemistry and Molecular Biology - December 26, 2019 Category: Biochemistry Source Type: research

Plasma oxysterol levels in luminal subtype breast cancer patients are associated with clinical data
In conclusion, this study shows, for the first time, that circulating levels of oxysterols, especially cholestane-3β,5α,6β-triol, may have prognostic roles in patients with luminal subtype breast cancer. (Source: The Journal of Steroid Biochemistry and Molecular Biology)
Source: The Journal of Steroid Biochemistry and Molecular Biology - December 24, 2019 Category: Biochemistry Source Type: research

Bacterial steroid-17,20-desmolase is a taxonomically rare enzymatic pathway that converts prednisone to 1,4-androstanediene-3,11,17-trione, a metabolite that causes proliferation of prostate cancer cells
Publication date: Available online 20 December 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Lindsey K. Ly, Joe L. Rowles, Hans Müller Paul, João M.P. Alves, Camdon Yemm, Patricia M. Wolf, Saravanan Devendran, Matthew E. Hudson, David J. Morris, John W. Erdman, Jason M. RidlonAbstractThe adrenal gland has traditionally been viewed as a source of “weak androgens”; however, emerging evidence indicates 11-oxy-androgens of adrenal origin are metabolized in peripheral tissues to potent androgens. Also emerging is the role of gut bacteria in the conversion of C21 glucocor...
Source: The Journal of Steroid Biochemistry and Molecular Biology - December 21, 2019 Category: Biochemistry Source Type: research

Vitamin D Receptor Fokl polymorphism is a determinant of both maternal and neonatal Vitamin D concentrations at birth
Publication date: Available online 20 December 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Spyridon N. Karras, Theocharis Koufakis, Vasiliki Antonopoulou, Dimitrios G. Goulis, Merve Alaylıoğlu, Erdinc Dursun, Diugy Gezen-Ak, Cedric Annweiler, Stefan Pilz, Hana Fakhoury, Fatme Al Anouti, Vikentia Harizopoulou, Declan P. Naughton, Pantelis Zebekakis, Kalliopi KotsaAbstractMaternal vitamin D deficiency is considered to be the key determinant of the development of neonatal vitamin D deficiency at birth and during early infancy. Specific vitamin D receptor (VDR) gene polymorphisms have been...
Source: The Journal of Steroid Biochemistry and Molecular Biology - December 21, 2019 Category: Biochemistry Source Type: research

Vitamin D deficiency in Kazakhstan: cross-sectional study
This study objected to evaluate Vitamin D status in adult population of both sexes residing in different geographical areas of Kazakhstan and to elucidate the possible contributing factors related to VDD.This cross-sectional study covered 6 regions of Kazakhstan and applied the systematic random sampling to recruit 1,347 healthy adults (of whom 819 were females) with mean age 44 ± 14 years. The concentration of 25-hydroxy vitamin D (25-OHD) was measured from May 2018 to August 2018 with Architect 25OH Vitamin D assay (Abbott Ireland Diagnostics Division Lisnamuck, Longford Co. Longford Ireland). Vitami...
Source: The Journal of Steroid Biochemistry and Molecular Biology - December 6, 2019 Category: Biochemistry Source Type: research