Oxysterols and the NeuroVascular Unit (NVU): a far true love with bright and dark sides
Publication date: Available online 23 April 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Julien Saint-Pol, Fabien GosseletAbstractThe brain is isolated from the whole body by the blood-brain barrier (BBB) which is located in brain microvessel endothelial cells (ECs). Through physical and metabolic properties induced by brain pericytes, astrocytes and neurons (these cells and the ECs referred to as the neurovascular unit (NVU)), the BBB hardly restricts exchanges of molecules between the brain and the bloodstream. Among them, cholesterol exchanges between these two compartments are very li...
Source: The Journal of Steroid Biochemistry and Molecular Biology - April 25, 2019 Category: Biochemistry Source Type: research

IGF-1 reverses the osteogenic inhibitory effect of dexamethasone on BMP9-induced osteogenic differentiation in mouse embryonic fibroblasts via PI3K/AKT/COX-2 pathway
This study may provide new clinical management strategy for GIOP cases. (Source: The Journal of Steroid Biochemistry and Molecular Biology)
Source: The Journal of Steroid Biochemistry and Molecular Biology - April 22, 2019 Category: Biochemistry Source Type: research

High expression of adrenal P450 aromatase (CYP19A1) in association with ARMC5-primary bilateral macronodular adrenocortical hyperplasia
Publication date: Available online 20 April 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Annabel Berthon, Fady Hannah-Shmouni, Andrea Gutierrez Maria, Fabio R. Faucz, Constantine A. StratakisAbstractPrimary bilateral macronodular adrenocortical hyperplasia (PBMAH) is a rare cause of ACTH-independent Cushing syndrome (CS), which has been associated with ectopic G-protein coupled receptors (GPCRs) in the adrenal cortex. We recently studied a 51-year-old male with PBMAH who presented with severe CS and hyperestronemia, manifesting clinically with a cushingoid appearance, gynecomastia, telang...
Source: The Journal of Steroid Biochemistry and Molecular Biology - April 22, 2019 Category: Biochemistry Source Type: research

Nuclear receptors, cholesterol homeostasis and the immune system
Publication date: Available online 16 April 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Sayyed Hamed Shahoei, Erik R. NelsonAbstractCholesterol is essential for maintaining membrane fluidity in eukaryotes. Additionally, the synthetic cascade of cholesterol results in precursor molecules important for cellular function such as lipid raft formation and protein prenylation. As such, cholesterol homeostasis is tightly regulated. Interestingly, it is now known that some cholesterol precursors and many metabolites serve as active signaling molecules, binding to different classes of receptors i...
Source: The Journal of Steroid Biochemistry and Molecular Biology - April 17, 2019 Category: Biochemistry Source Type: research

An evaluation of total 25-hydroxyvitamin D assay standardization: Where are we today?
Publication date: June 2019Source: The Journal of Steroid Biochemistry and Molecular Biology, Volume 190Author(s): Lise Nørkjær Bjerg, Jens Romlund Halgreen, Svend Høime Hansen, Howard A. Morris, Niklas Rye JørgensenAbstractBackgroundSerum total 25-hydroxyvitamin D is a measure of the total circulating 25-hydroxyvitamin D concentration and is the primary measurement for estimating vitamin D status. A number of automated immunoassays are commercially available, and in an attempt to standardize the assays the Vitamin D Standardization Program (VDSP) was established in 2010. Therefore, the aim of th...
Source: The Journal of Steroid Biochemistry and Molecular Biology - April 17, 2019 Category: Biochemistry Source Type: research

Update on ERbeta
Publication date: Available online 14 April 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Jan-Ake Gustafsson, Anders Strom, Margaret WarnerAbstractERbeta (ERβ) celebrated its 20th birthday in 2016 and although the overwhelming data in the literature indicate a role for this receptor in the control of epithelial proliferation, neurodegeneration and immune function, no ERβ agonists have yet made it to the clinics. This is the situation, despite the fact that very good safe ERβ agonists have been synthesized and at least one has been donated to the NIH for distribution to resea...
Source: The Journal of Steroid Biochemistry and Molecular Biology - April 16, 2019 Category: Biochemistry Source Type: research

The role and mechanism of microbial 3-ketosteroid Δ1-dehydrogenases in steroid breakdown
Publication date: Available online 13 April 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Ali Rohman, Bauke W. DijkstraAbstract3-Ketosteroid Δ1-dehydrogenases are FAD-dependent enzymes that catalyze the introduction of a double bond between the C1 and C2 atoms of the A-ring of 3-ketosteroid substrates. These enzymes are found in a large variety of microorganisms, especially in bacteria belonging to the phylum Actinobacteria. They play a critical role in the early steps of the degradation of the steroid core. 3-Ketosteroid Δ1-dehydrogenases are of particular interest for the eti...
Source: The Journal of Steroid Biochemistry and Molecular Biology - April 14, 2019 Category: Biochemistry Source Type: research

Secosterol-B affects endoplasmic reticulum structure in endothelial cells
Publication date: Available online 13 April 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Francesca Luchetti, Rita Crinelli, Maria Gemma Nasoni, Erica Cesarini, Barbara Canonico, Loretta Guidi, Chiara Zerbinati, Mauro Magnani, Stefano Papa, Luigi IulianoAbstractOxysterols, oxidized derivatives of cholesterol found in LDL and atherosclerotic plaques, trigger several biological responses involved in the initiation and progression of atherosclerosis. Endothelial dysfunction, which occurs when vascular homeostasis is altered, plays a key role in the pathogenesis of several metabolic diseases. ...
Source: The Journal of Steroid Biochemistry and Molecular Biology - April 14, 2019 Category: Biochemistry Source Type: research

Performance of LC–MS/MS and immunoassay based 24-h urine free cortisol in the diagnosis of Cushing's syndrome
We examined 24-h urine samples from patients with surgically confirmed CS (n = 77; Cushing’s disease (n = 44), ectopic CS (n = 5), adrenal CS (n = 28)) and patients in whom Cushing’s syndrome was excluded (n = 97) by long-term follow up. UFC was first measured by automated chemiluminescence immunoassays (ADVIA Centaur, Siemens; LIAISON, DiaSorin). Aliquots of all samples were also analyzed by liquid chromatography-tandem mass spectrometry (LC–MS/MS). Statistics: Passing-Bablok Regression, Receiver operating characteristic (ROC) analysis...
Source: The Journal of Steroid Biochemistry and Molecular Biology - April 11, 2019 Category: Biochemistry Source Type: research

Flavonoids differentially modulate liver X receptors activity—Structure-function relationship analysis
Publication date: June 2019Source: The Journal of Steroid Biochemistry and Molecular Biology, Volume 190Author(s): Allan Fouache, Nada Zabaiou, Cyrille De Joussineau, Laurent Morel, Sandrine Silvente-Poirot, Amira Namsi, Gérard Lizard, Marc Poirot, Makoto Makishima, Silvère Baron, Jean-Marc A. Lobaccaro, Amalia TroussonAbstractLiver X receptors (LXRs) α (NR1H3) and β (NR1H2) are nuclear receptors that have been involved in the regulation of many physiological processes, principally in the control of cholesterol homeostasis, as well as in the control of the cell death and proliferation balance. Thes...
Source: The Journal of Steroid Biochemistry and Molecular Biology - April 10, 2019 Category: Biochemistry Source Type: research

Expression of aldosterone synthase CYP11B2 was inversely correlated with longevity
Publication date: Available online 8 April 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Taiki Hayashi, Zhen Zhang, Ghaith Al-Eyd, Atsushi Sasaki, Masami Yasuda, Masafumi Oyama, Celso E. Gomez-Sanchez, Hirotaka Asakura, Tsugio Seki, Kuniaki Mukai, Koshiro NishimotoAbstractImmunohistochemistry of human aldosterone synthase (CYP11B2) has revealed that most of aldosterone is autonomously produced in aldosterone-producing cell clusters (APCCs) beneath the capsule of adult adrenals rather than physiologically in the zona glomerulosa (ZG). APCCs have been occasionally found to harbor a somatic m...
Source: The Journal of Steroid Biochemistry and Molecular Biology - April 10, 2019 Category: Biochemistry Source Type: research

Clinical spectrum of Li-Fraumeni syndrome/Li-Fraumeni-like syndrome in Brazilian individuals with the TP53 p.R337H mutation
Publication date: Available online 8 April 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Amanda Meneses Ferreira, Maria Candida Fragoso (Source: The Journal of Steroid Biochemistry and Molecular Biology)
Source: The Journal of Steroid Biochemistry and Molecular Biology - April 10, 2019 Category: Biochemistry Source Type: research

Assessment of free testosterone concentration
Publication date: Available online 7 April 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Brian G Keevil, Jo AdawayAbstractTestosterone (T) is strongly bound to sex hormone binding globulin and measurement of free T may be more appropriate than measuring total serum T, according to the free hormone theory. This view remains controversial and it has its detractors who claim that little extra benefit is gained than simply measuring total T, but it is endorsed by recent clinical practice guidelines for investigation of androgen disorders in both men and women. Free T measurement is very challe...
Source: The Journal of Steroid Biochemistry and Molecular Biology - April 8, 2019 Category: Biochemistry Source Type: research

Presence of the vitamin D inactivating enzyme CYP24A1 in human sperm and prediction of the success of intrauterine insemination: a prospective study
Publication date: Available online 6 April 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Lasse Bøllehuus Hansen, Mette Lorenzen, Ursula Bentin-Ley, John Erik Nielsen, Hans Krog, Anders Hayden Berg, Bonnie Sofie Håkansson, Astrid Munk Pedersen, Thomas Høst, Anders Juul, Martin Blomberg JensenAbstractMale fertility is routinely evaluated by semen analysis, although semen quality variables such as; sperm count, motility and morphology have low predictive value for spontaneous pregnancies and fertility treatment outcomes. Vitamin D has been suggested to be beneficial for ma...
Source: The Journal of Steroid Biochemistry and Molecular Biology - April 8, 2019 Category: Biochemistry Source Type: research

Identification of the fungicide epoxiconazole by virtual screening and biological assessment as inhibitor of human 11β-hydroxylase and aldosterone synthase
This study applied in silico screening and in vitro evaluation for the discovery of xenobiotics inhibiting CYP11B1 and CYP11B2. Several databases comprising environmentally relevant pollutants, chemicals in body care products, food additives and drugs were virtually screened using CYP11B1 and CYP11B2 pharmacophore models. A first round of biological testing used hamster cells overexpressing human CYP11B1 or CYP11B2 to analyze 25 selected virtual hits. Three compounds inhibited CYP11B1 and CYP11B2 with IC50 values below 3 µM. The most potent inhibitor was epoxiconazole (IC50 value of 623 nM for CYP11B1 and 113&...
Source: The Journal of Steroid Biochemistry and Molecular Biology - April 8, 2019 Category: Biochemistry Source Type: research

Circulating 11-oxygenated androgens across species
This study provides a comprehensive analysis of a set of C19 steroids, including the traditional androgens and 11-oxyandrogens, across 18 animal species. As previously shown, serum DHEA and DHEAS were much higher in primates than all other species. Circulating 11-oxyandrogens, especially 11KT, were observed in notable amounts in male, but not in female trout, consistent with gonadal origin in fish. The circulating concentrations of 11-oxyandrogens ranged from 0.1 to 10 nM in pigs, guinea pigs and in all the primates studied (rhesus macaque, baboon, chimpanzee and human) but not in rats or mice, and 11OHA4 was consis...
Source: The Journal of Steroid Biochemistry and Molecular Biology - April 7, 2019 Category: Biochemistry Source Type: research

The adrenal gland in stress – adaptation on a cellular level
Publication date: Available online 5 April 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Ilona Berger, Martin Werdermann, Stefan R. Bornstein, Charlotte SteenblockAbstractHuman individuals are constantly confronted to various kinds of stressors and the body’s response and adaptation is essential for human health. The adrenal gland as the main producer of stress hormones plays a major role in the response to physiological challenges and is able to adapt to these physiological needs. Proper adaptation is of particular importance since dysregulation of the stress system is the cause of ...
Source: The Journal of Steroid Biochemistry and Molecular Biology - April 7, 2019 Category: Biochemistry Source Type: research

Major glucuronide metabolites of testosterone are primarily transported by MRP2 and MRP3 in human liver, intestine and kidney
Publication date: Available online 5 April 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Cindy Yanfei Li, Abdul Basit, Anshul Gupta, Zsuzsanna Gáborik, Emese Kis, Bhagwat PrasadAbstractTestosterone glucuronide (TG), androsterone glucuronide (AG), etiocholanolone glucuronide (EtioG) and dihydrotestosterone glucuronide (DHTG) are the major metabolites of testosterone (T), which are excreted in urine and bile. Glucuronides can be deconjugated to active androgen in gut lumen after biliary excretion, which in turn can affect physiological levels of androgens. The goal of this study was t...
Source: The Journal of Steroid Biochemistry and Molecular Biology - April 7, 2019 Category: Biochemistry Source Type: research

Performance of LC-MS/MS and immunoassay based 24-h urine free cortisol in the diagnosis of Cushing's syndrome
We examined 24-h urine samples from patients with surgically confirmed CS (n = 77; Cushing’s disease (n = 44), ectopic CS (n = 5), adrenal CS (n = 28)) and patients in whom Cushing’s syndrome was excluded (n = 97) by long-term follow up. UFC was first measured by automated chemiluminescence immunoassays (ADVIA Centaur, Siemens; LIAISON, DiaSorin). Aliquots of all samples were also analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Statistics: Passing-Bablok Regression, Receiver operating characteristic (ROC) analysis with ...
Source: The Journal of Steroid Biochemistry and Molecular Biology - April 7, 2019 Category: Biochemistry Source Type: research

Flavonoids differentially modulate Liver X Receptors activity. Structure-function relationship analysis
Publication date: Available online 5 April 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Allan Fouache, Nada Zabaiou, Cyrille De Joussineau, Laurent Morel, Sandrine Silvente-Poirot, Amira Namsi, Gérard Lizard, Marc Poirot, Makoto Makishima, Silvère Baron, Jean-Marc A. Lobaccaro, Amalia TroussonAbstractLiver X receptors (LXRs) α (NR1H3) and β (NR1H2) are nuclear receptors that have been involved in the regulation of many physiological processes, principally in the control of cholesterol homeostasis, as well as in the control of the cell death and proliferation balan...
Source: The Journal of Steroid Biochemistry and Molecular Biology - April 7, 2019 Category: Biochemistry Source Type: research

Sp1 Regulates Steroidogenic Genes and LHCGR Expression in Primary Human Luteinized Granulosa Cells
This study aimed to characterize the expression of LHCGR mRNA in primary human luteinized granulosa cells (hLGCs) obtained from patients undergoing in vitro fertilization and to correlate LHCGR expression with the response of hLGCs to hCG by assessing the expression of genes known to be markers of hCG actions. The results show that LHCGR expression is low in freshly isolated cells but recovers rapidly in culture and that hCG maintains LHCGR expression, suggesting a positive feedback loop. The activity of a LHCGR-LUC reporter increased in cells treated with hCG but not with follicle-stimulating hormone. Treatment with hCG a...
Source: The Journal of Steroid Biochemistry and Molecular Biology - April 5, 2019 Category: Biochemistry Source Type: research

Isolation and functional characterization of a novel endogenous inverse agonist of estrogen related receptors (ERRs) from human pregnancy urine
Publication date: Available online 5 April 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Faegheh Ghanbari, Andrea Hebert-Losier, Janelle Barry, Donald Poirier, Vincent Giguere, Sylvie Mader, Anie PhilipAbstractEstrogen-receptor related receptors (ERRs) which consists of ERRα, ERRβ and ERRγ belong to the orphan nuclear receptor subfamily 3, group B (NR3B) subfamily, and are constitutively active. ERRs have been shown to actively modulate estrogenic responses, and to play an essential role in pregnancy, and are implicated in breast cancer progression. Despite intensive effor...
Source: The Journal of Steroid Biochemistry and Molecular Biology - April 5, 2019 Category: Biochemistry Source Type: research

Alterations in cholesterol metabolism as a risk factor for developing Alzheimer’s disease: Potential novel targets for treatment
Publication date: June 2019Source: The Journal of Steroid Biochemistry and Molecular Biology, Volume 190Author(s): Raúl Loera-Valencia, Julen Goikolea, Cristina Parrado-Fernandez, Paula Merino-Serrais, Silvia MaioliAbstractAlzheimer's disease (AD) is the most common form of dementia and it is characterized by the deposition of amyloid-β (Aβ) plaques and neurofibrillary tangles in the brain. However, the complete pathogenesis of the disease is still unknown. High level of serum cholesterol has been found to positively correlate with an increased risk of dementia and some studies have reported a decreased pr...
Source: The Journal of Steroid Biochemistry and Molecular Biology - April 3, 2019 Category: Biochemistry Source Type: research

First international descriptive and interventional survey for cholesterol and non-cholesterol sterol determination by gas- and liquid-chromatography - Urgent need for harmonisation of analytical methods
Publication date: Available online 30 March 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Dieter Lütjohann, Ingemar Björkhem, Silvia Friedrichs, Anja Kerksiek, Anita Lövgren-Sandblom, Wolf-Jochen Geilenkeuser, Robert Ahrends, Isabel Andrade, Diana Ansorena, Iciar Astiasarán, Lucía Baila-Rueda, Bianca Barriuso, Susen Becker, Lionel Bretillon, Richard W. Browne, Claudio Caccia, Uta Ceglarek, Ana Cenarro, Peter J. Crick, Günter FaulerAbstractSerum concentrations of lathosterol, the plant sterols campesterol and sitosterol and the cholesterol metabolite 5&alph...
Source: The Journal of Steroid Biochemistry and Molecular Biology - March 31, 2019 Category: Biochemistry Source Type: research

An evaluation of total 25-hydroxyvitamin D assay standardization; where are we today?
Publication date: Available online 30 March 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Lise Nørkjær Bjerg, Jens Romlund Halgreen, Svend Høime Hansen, Howard A. Morris, Niklas Rye JørgensenAbstractBackgroundSerum total 25-hydroxyvitamin D is a measure of the total circulating 25-hydroxyvitamin D concentration and is the primary measurement for estimating vitamin D status. A number of automated immunoassays are commercially available, and in an attempt to standardize the assays the Vitamin D Standardization Program (VDSP) was established in 2010. Therefore, the ...
Source: The Journal of Steroid Biochemistry and Molecular Biology - March 31, 2019 Category: Biochemistry Source Type: research

Highlights from the 21st Workshop on Vitamin D in Barcelona, May 2018
Publication date: May 2019Source: The Journal of Steroid Biochemistry and Molecular Biology, Volume 189Author(s): James C. Fleet, Moray J. Campbell, Carsten Carlberg, Marie B. Demay, Peter R. Ebeling, Inez Schoenmakers, John H. White, JoEllen Welsh, Martin Hewison (Source: The Journal of Steroid Biochemistry and Molecular Biology)
Source: The Journal of Steroid Biochemistry and Molecular Biology - March 30, 2019 Category: Biochemistry Source Type: research

Expression and regulation of GnRHR2 gene and testosterone secretion mediated by GnRH2 and GnRHR2 within porcine testes
This study also explores the regulation mechanism of testosterone secretion mediated by GnRH2 and GnRHR2 in porcine Leydig cells.Graphical abstract (Source: The Journal of Steroid Biochemistry and Molecular Biology)
Source: The Journal of Steroid Biochemistry and Molecular Biology - March 30, 2019 Category: Biochemistry Source Type: research

Paradoxical effect of testosterone supplementation therapy on cardiac ischemia/reperfusion injury in aged rats
Publication date: Available online 28 March 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Fernando A.C. Seara, Raiana A.Q. Barbosa, Marcus V.N. Santos, Ainá E. Domingos, Gustavo Monnerat, Adriana B. Carvalho, Emerson L. Olivares, José G. Mill, Jose H.M. Nascimento, Antonio C. Campos de CarvalhoAbstractAging is followed by numerous physiological limitations that reduce health span, particularly cardiovascular and metabolic disorders. Testosterone supplementation therapy (TST) has been widely used in the treatment of aging dysfunctions in either adult or aged patients, although...
Source: The Journal of Steroid Biochemistry and Molecular Biology - March 30, 2019 Category: Biochemistry Source Type: research

Modulation of exercise training related adaptation of body composition and regulatory pathways by anabolic steroids
This study aims at investigating the changes of body composition and the expression of selected genes and pathways essential for this adaptation process. Therefore, male wistar rats were treated with the anabolic steroid metandienone in two groups (n = 16; metandienone, metandienone + exercise) alongside with control groups (n = 16; control, exercise). Following a 6-week steep-angle treadmill training protocol, weight of organs, visceral fat and muscles was determined. M. gastrocnemius was histologically assessed by ATPase staining, mRNA and protein levels of factors of regeneratio...
Source: The Journal of Steroid Biochemistry and Molecular Biology - March 27, 2019 Category: Biochemistry Source Type: research

Progesterone Receptor Isoform B Expression in Pulmonary Neuroendocrine Cells Decreases Cell Proliferation
Publication date: Available online 26 March 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Teeranut Asavasupreechar, Ryoko Saito, Dean P. Edwards, Hironobu Sasano, Viroj BoonyaratanakornkitAbstractThe progesterone receptor (PR) has been reported to play important roles in lung development and function, such as alveolarization, alveolar fluid clearance (AFC) and upper airway dilator muscle activity. In the lung, pulmonary neuroendocrine cells (PNECs) are important in the etiology and progression of lung neuroendocrine tumors (NETs). Women with lung NETs had significantly better survival rate...
Source: The Journal of Steroid Biochemistry and Molecular Biology - March 27, 2019 Category: Biochemistry Source Type: research

Highlights from the 21th Workshop on Vitamin D in Barcelona, May 2018
Publication date: Available online 26 March 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): James C. Fleet, Moray J. Campbell, Carsten Carlberg, Marie B. Demay, Peter R. Ebeling, Inez Schoenmakers, John White, JoEllen Welsh, Martin Hewison (Source: The Journal of Steroid Biochemistry and Molecular Biology)
Source: The Journal of Steroid Biochemistry and Molecular Biology - March 27, 2019 Category: Biochemistry Source Type: research

Defining Vitamin D Status Using Multi-Metabolite Mathematical Modelling: A Pregnancy Perspective
Publication date: Available online 26 March 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): C.H.L. Beentjes, J.P. Taylor-King, A. Bayani, C.N. Davis, J.L. Dunster, S. Jabbari, G.R. Mirams, C. Jenkinson, M. Kilby, M. Hewison, J.A. TamblynAbstractVitamin D deficiency is linked to adverse pregnancy outcomes such as pre-eclampsia (PET) but remains defined by serum measurement of 25-hydroxyvitamin D3 (25(OH)D3) alone. To identify broader changes in vitamin D metabolism during normal and PET pregnancies we developed a relatively simple but fully parametrised mathematical model of the vitamin D met...
Source: The Journal of Steroid Biochemistry and Molecular Biology - March 27, 2019 Category: Biochemistry Source Type: research

Vitamin D3 mediated regulation of steroidogenesis mitigates testicular activity in an aged rat model
Publication date: Available online 25 March 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Malsawmhriatzuala Jeremy, Guruswami Gurusubramanian, Vikas Kumar RoyAbstractTesticular aging leads to a decrease in spermatogenesis and steroidogenesis. Vitamin D plays an important role in reproduction by modulating testicular steroidogenesis. As the role of vitamin D3 in testicular steroidogenesis during aging has not been explored, the aim of this study was to evaluate the effects of vitamin D3 on testicular functions in D-gal-induced aged rats. Vitamin D3 treatment on D-gal-induced aged rats resul...
Source: The Journal of Steroid Biochemistry and Molecular Biology - March 26, 2019 Category: Biochemistry Source Type: research

A comprehensive method to determine sterol species in human faeces by GC-triple quadrupole MS
Publication date: Available online 25 March 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Sonja Kunz, Silke MatysikAbstractThe human gut microbiome plays a crucial role in both health and disease. Metabolites in human faeces related to microbial activity might therefore be attractive surrogate markers to track changes of microbiota induced by diet or disease.The hyphenation of gas chromatography with triple quadrupole mass spectrometry is a promising approach to increase sensitivity and selectivity as compared to single quad MS instruments. The versatility of gas chromatography-tandem mass...
Source: The Journal of Steroid Biochemistry and Molecular Biology - March 26, 2019 Category: Biochemistry Source Type: research

Tacalcitol increases the sensitivity of colorectal cancer cells to 5-fluorouracil by downregulating the thymidylate synthase
In conclusion, we suggest that both VDR and CaSR might be useful as molecular markers for predicting treatment outcomes and identifying the CRC patient subgroups who might benefit from 5-FU-based chemotherapy combined with vitamin D analog. (Source: The Journal of Steroid Biochemistry and Molecular Biology)
Source: The Journal of Steroid Biochemistry and Molecular Biology - March 26, 2019 Category: Biochemistry Source Type: research

A phase inversion based nanoemulsion fabrication process to encapsulate vitamin D3 for food applications
Publication date: Available online 25 March 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Vaibhav Kumar Maurya, Manjeet AggarwalAbstract•A phase inversion based nanoemulsion fabrication process was developed to encapsulate vitamin D3 by blending caprylic-/capric triglyceride (CCTG), Leciva S70, Kolliphor® HS 15, vitamin D3 and aqueous phase (sodium chloride solution). In order to find out nanoparticle formation zone (NFZ), a ternary diagram was plotted with 41 possible combinations of three components CCTG, Kolliphor® HS 15 and aqueous phase. Out of forty one, only twelve comb...
Source: The Journal of Steroid Biochemistry and Molecular Biology - March 26, 2019 Category: Biochemistry Source Type: research

Hydroperoxy steroids and triterpenoids derived from plant and fungi: Origin, structures and biological activities
Publication date: Available online 25 March 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Vera A. Vil, Alexander O. Terent’ev, Nick Savidov, Tatyana A. Gloriozova, Vladimir V. Poroikov, Tatyana A. Pounina, Valery M. DembitskyAbstractHydroperoxides (R-OOH) represent a small family of natural metabolites that have been isolated from higher plants, fungi, and marine organisms. This paper is devoted to the distribution of hydroperoxides in plants, fungi and terrestrial fungal endophytes and their biological activity. Hydroperoxides of plants demonstrate a wide range of biological activit...
Source: The Journal of Steroid Biochemistry and Molecular Biology - March 26, 2019 Category: Biochemistry Source Type: research

combined oral contraceptive IN FEMALE MICE CAUSES HYPERINSULINEMIA DUE TO β-cell HYPERSECRETION AND REDUCTION IN INSULIN CLEARANCE
Publication date: Available online 25 March 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Cremilda Amaral Roso de Oliveira, Thiago dos Reis Araujo, Gésily de Souza Aguiar, Joel Alves da Silva Junior, Jean Franciesco Vettorazzi, Israelle Netto Freitas, Kênia Moreno de Oliveira, Antonio Carlos Boschero, Maria Lúcia Bonfleur, Júlia Rosauro Clarke, Helene Nara Henriques, Rosane Aparecida RibeiroAbstractOral contraception is the most commonly used interventional method in the world. However, several women employ the continuous use of these hormones to avoid pre- and m...
Source: The Journal of Steroid Biochemistry and Molecular Biology - March 26, 2019 Category: Biochemistry Source Type: research

Aerobic exercise training and dehydroepiandrosterone administration increase testicular sex steroid hormones and enhance reproductive function in high-sucrose-induced obese rats
This study assessed the effects of chronic dehydroepiandrosterone (DHEA) administration and exercise training on testicular sex steroid hormone levels and reproductive function in high-sucrose induced obese rats. After 14 weeks of a high-sucrose diet, Wistar male rats were assigned randomly to the control, exercise training (running at 25 m/min for 1 h, 5 days/week), DHEA administration, and combined exercise training and DHEA administration groups (n = 7 each group). Six weeks of DHEA administration and/or exercise training significantly increased plasma concentrations of DHEA and 5α-dihy...
Source: The Journal of Steroid Biochemistry and Molecular Biology - March 26, 2019 Category: Biochemistry Source Type: research

Age-dependent expression of the Vitamin D receptor and the protective effect of Vitamin D receptor activation on H2O2-induced apoptosis in rat intervertebral disc cells
Publication date: Available online 21 March 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Tong Tong, Zhihui Liu, Hua Zhang, Jinglei Sun, Dongxia Zhang, Feng Wang, Dechao Miao, Yong ShenAbstractAccumulating evidence shows that genetic polymorphism of the vitamin D receptor (VDR) gene is associated with intervertebral disc degeneration (IDD), implying that VDR may be involved in the pathogenesis of IDD. However, the exact relationship between VDR and IDD remains unknown. The aim of this study was to investigate the age-dependent expression of VDR in rat intervertebral discs and to determine ...
Source: The Journal of Steroid Biochemistry and Molecular Biology - March 22, 2019 Category: Biochemistry Source Type: research

Enzymatic interconversion of the oxysterols 7β,25-dihydroxycholesterol and 7-keto,25-hydroxycholesterol by 11β-hydroxysteroid dehydrogenase type 1 and 2
In conclusion, these results demonstrate a novel glucocorticoid-independent pre-receptor regulation mediated by 11β-HSDs.Graphical abstract (Source: The Journal of Steroid Biochemistry and Molecular Biology)
Source: The Journal of Steroid Biochemistry and Molecular Biology - March 21, 2019 Category: Biochemistry Source Type: research

Comparison of Free and Total 25-hydroxyvitamin D in Normal Human Pregnancy
Publication date: Available online 20 March 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Oleg Tsuprykov, Claudia Buse, Roman Skoblo, Berthold HocherAbstractVitamin D status correct monitoring during pregnancy is critically important for both maternal and fetal health. 25-hydroxyvitamin D (25(OH)D) – a prohormone of a biologically active 1,25-dihydroxyvitamin D (1,25(OH)2D), despite the lack of biological activity, during the past decades has been routinely used as a main biomarker characterizing vitamin D status. About 85% of 25(OH)D in the bloodstream is bound to its specific carri...
Source: The Journal of Steroid Biochemistry and Molecular Biology - March 21, 2019 Category: Biochemistry Source Type: research

The Isoflavones Genistein and Daidzein Increase Hepatic Concentration of Thyroid Hormones and Affect Cholesterol Metabolism in Middle-Aged Male Rats
We examined whether isoflavones interfere with thyroid homeostasis, increase hepatic thyroid hormone concentrations and affect cholesterol metabolism in middle-aged (MA) male rats. Thirteen-month-old Wistar rats were injected subcutaneously with 35 mg/kg b.w./day of genistein, daidzein or vehicle (controls) for four weeks. Hepatic Dio1 gene expression was up-regulated by 70% (p 
Source: The Journal of Steroid Biochemistry and Molecular Biology - March 16, 2019 Category: Biochemistry Source Type: research

Alterations in cholesterol metabolism as a risk factor for developing Alzheimer's disease: Potential novel targets for treatment
Publication date: Available online 13 March 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Raúl Loera-Valencia, Julen Goikolea, Cristina Parrado-Fernandez, Paula Merino-Serrais, Silvia MaioliAbstractAlzheimer's disease (AD) is the most common form of dementia and it is characterized by the deposition of amyloid-β (Aβ) plaques and neurofibrillary tangles in the brain. However, the complete pathogenesis of the disease is still unknown. High level of serum cholesterol has been found to positively correlate with an increased risk of dementia and some studies have reported a dec...
Source: The Journal of Steroid Biochemistry and Molecular Biology - March 15, 2019 Category: Biochemistry Source Type: research

Editorial Board
Publication date: April 2019Source: The Journal of Steroid Biochemistry and Molecular Biology, Volume 188Author(s): (Source: The Journal of Steroid Biochemistry and Molecular Biology)
Source: The Journal of Steroid Biochemistry and Molecular Biology - March 15, 2019 Category: Biochemistry Source Type: research

Alterations in cholesterol metabolism as a risk factor for developing Alzheimer´s disease: Potential novel targets for treatment
Publication date: Available online 13 March 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Raúl Loera-Valencia, Julen Goikolea, Cristina Parrado-Fernandez, Paula Merino-Serrais, Silvia MaioliAbstractAlzheimer's disease (AD) is the most common form of dementia and it is characterized by the deposition of amyloid-β (Aβ) plaques and neurofibrillary tangles in the brain. However, the complete pathogenesis of the disease is still unknown. High level of serum cholesterol has been found to positively correlate with an increased risk of dementia and some studies have reported a dec...
Source: The Journal of Steroid Biochemistry and Molecular Biology - March 13, 2019 Category: Biochemistry Source Type: research

Screening of Novel 3α5β-Neurosteroids for Neuroprotective Activity against Glutamate- or NMDA-Induced Excitotoxicity
Publication date: Available online 11 March 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Marketa Smidkova, Miroslav Hajek, Santosh Kumar Adla, Barbora Slavikova, Hana Chodounska, Marika Matousova, Helena Mertlikova-Kaiserova, Eva KudovaAbstractA broad variety of central nervous system diseases have been associated with glutamate induced excitotoxicity under pathological conditions. The neuroprotective effects of neurosteroids can combat this excitotoxicity. Herein, we have demonstrated the neuroprotective effect of novel steroidal N-methyl-D-aspartate receptor inhibitors against glutamate...
Source: The Journal of Steroid Biochemistry and Molecular Biology - March 12, 2019 Category: Biochemistry Source Type: research

Structural and energetic basis for novel epicatechin derivatives acting as GPER agonists through the MMGBSA method
Publication date: Available online 6 March 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Martiniano Bello, David Méndez-Luna, Viviana Sarmiento, José Correa Basurto, Nayelli Najera, Francisco Villarreal, Guillermo CeballosAbstract(-)-Epicatechin (Epi) has been demonstrated to activate pathways involved in GPER-stimulated nitric oxide (NO) production via endothelial NO synthase, known as the eNOS/NO pathway. Previous studies combining synthesis of four Epi derivatives demonstrated that Epi and Epi-prop, Epi-4-prop and Epi-5-prop were able to bind GPER by acting as GPER agonist...
Source: The Journal of Steroid Biochemistry and Molecular Biology - March 8, 2019 Category: Biochemistry Source Type: research

Resveratrol and other dietary polyphenols are inhibitors of estrogen metabolism in human breast cancer cells
Publication date: Available online 6 March 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Stefan Poschner, Alexandra Maier-Salamon, Theresia Thalhammer, Walter JägerAbstractPolyphenols in foods and dietary supplements are commonly used for the prevention and treatment of a variety of malignancies, including breast cancer. However, daily intake by patients with breast cancer is controversial, as these compounds may stimulate cancer growth. Estrogens serve key roles in breast cancer cell proliferation; therefore, understanding the interaction between endogenous steroid hormones and natur...
Source: The Journal of Steroid Biochemistry and Molecular Biology - March 8, 2019 Category: Biochemistry Source Type: research

Estrogens and breast cancer: Mechanisms involved in obesity-related development, growth and progression
Publication date: Available online 6 March 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Priya Bhardwaj, CheukMan C. Au, Alberto Benito-Martin, Heta Ladumor, Sofya Oshchepkova, Ruth Moges, Kristy A. BrownAbstractObesity is a risk factor for estrogen receptor-positive (ER+) breast cancer after menopause. The pro-proliferative effects of estrogens are well characterized and there is a growing body of evidence to also suggest an important role in tumorigenesis. Importantly, obesity not only increases the risk of breast cancer, but it also increases the risk of recurrence and cancer-associated...
Source: The Journal of Steroid Biochemistry and Molecular Biology - March 8, 2019 Category: Biochemistry Source Type: research