Filtered By:
Specialty: Microbiology

This page shows you your search results in order of relevance.

Order by Relevance | Date

Total 170 results found since Jan 2013.

Targeted siRNA-immunoliposomes as a promising therapeutic agent against highly pathogenic avian influenza A (H5N1) virus infection.
This study describes a proof of concept study on the use of siRNA-immunoliposomes as a therapeutic agent against H5N1 influenza virus infection. SiRNA specific for influenza virus nucleoprotein (NP) mRNA was employed as the key antiviral agent to inhibit viral replication in this study. A humanized single-chain Fv antibody (huscFv) against the hemagglutinin (HA) of H5N1 highly pathogenic avian influenza (HPAI) was used as the targeting molecule to HA of H5N1 virus, which is abundantly expressed on the surface of infected cells. The huscFv was decorated onto cationic PEGylated DC-Chol/DOPE liposomes to generate immunoliposo...
Source: Antimicrobial Agents and Chemotherapy - March 10, 2014 Category: Microbiology Authors: Khantasup K, Kopermsub P, Chaichoun K, Dharakul T Tags: Antimicrob Agents Chemother Source Type: research

Cystathionine-gamma-lyase gene silencing with siRNA in monocytes/macrophages protects mice against acute pancreatitis.
Abstract Hydrogen sulphide (H2S) is an endogenous inflammatory mediator produced by cystathionine-γ-lyase (CSE) in monocytes/macrophages. To determine the role of H2S and macrophages in inflammation, we used small interference RNA (siRNA) to target the CSE gene and investigated its effect in a mouse model of acute pancreatitis. Acute pancreatitis is characterised by increased levels of plasma amylase, myeloperoxidase (MPO) activity and pro-inflammatory cytokines and chemokines in the pancreas and lung. SiRNA treatment attenuated inflammation in the pancreas and lungs of mice following caerulein-induced acute panc...
Source: Applied Microbiology and Biotechnology - September 28, 2015 Category: Microbiology Authors: Badiei A, Chambers ST, Gaddam RR, Fraser R, Bhatia M Tags: Appl Microbiol Biotechnol Source Type: research

A novel conditional gene silencing method using a tumor-specific and heat-inducible siRNA system.
In this study, we inserted three kinds of HSE into tumor specific promoter hTERT, which aims to construct a temperature-inducible and tumor-specific RNAi plasmid vector. In our system, the expression of mature siRNA is tightly controlled by the heat shock-inducible and tumor-specific promoters. From the expression level of RNA and protein, we determined the efficiency of the inducible siRNA system by targeting SNCG gene in HepG2 and MCF-7 cells. Results showed that the controllable siRNA system could be induced to initiate siRNA expression by heat-induce. The silencing effect of SNCG is on a relative low level (10 %) at 3...
Source: Journal of Industrial Microbiology and Biotechnology - March 30, 2016 Category: Microbiology Authors: Feng J, Wang X, Liao Y, Feng J, Tang L Tags: J Ind Microbiol Biotechnol Source Type: research

Silencing the target rhoptry neck protein (RON) gene responsible for the invasion of Toxoplasma gondii with siRNA transfection.
Authors: Yürük M, Aksoy T, Sivcan E, Nergiz H Abstract Toxoplasma gondii is a coccidian protozoan that causes toxoplasmosis is a common disease in Turkey as well as all over the world. It causes various clinical symptoms depending on the immune system status, age, or location of the disease. There is an organelle called the apical complex at the anterior end of the parasite. Rhoptry Neck Proteins (RONs), a component of this organelle, play a critical role in the formation of "moving junction" and parasitophorous vacuoles during host cell invasion. On the other hand, interfering RNA (iRNA) treatment options develo...
Source: Mikrobiyoloji Bulteni - January 27, 2019 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

siRNA Screen of Early Poxvirus Genes Identifies the AAA+ ATPase D5 as the Virus Genome-Uncoating Factor.
Abstract Poxvirus genome uncoating is a two-step process. First, cytoplasmic viral cores are activated and early viral genes are expressed. Next, cores are disassembled and the genomes released. This second step depends on an early viral factor(s) that has eluded identification for over 40 years. We used a large-scale, high-throughput RNAi screen directed against vaccinia virus (VACV) to identify the VACV AAA+ ATPase D5 as the poxvirus uncoating factor. We show that the ATPase activity of D5 is required for uncoating. Superresolution microscopy suggests that D5 acts directly at viral cores for genome release. Thus...
Source: Cell Host and Microbe - January 15, 2014 Category: Microbiology Authors: Kilcher S, Schmidt FI, Schneider C, Kopf M, Helenius A, Mercer J Tags: Cell Host Microbe Source Type: research

Characterization of Novel Aptamers Specifically Directed to Red-Spotted Grouper Nervous Necrosis Virus (RGNNV)-Infected Cells for Mediating Targeted siRNA Delivery
In this study, we generated three aptamers against red-spotted grouper nervous necrosis virus (RGNNV)-infected grouper brain (GB) cells using the Cell-SELEX (cell based-systematic evolution of ligands by exponential enrichment) technology. The selected aptamers formed stable stem-loop structures, and could specifically recognize RGNNV-infected GB cells, with calculated dissociation constants (Kd) of 27.96, 29.3, and 59.5 nM for aptamers GBN2, GBN10, and GBN34, respectively. They also recognized RGNNV-infected brain tissues. The three aptamers were non-toxic and showed antiviral activities both in vitro and in vivo. Fluores...
Source: Frontiers in Microbiology - April 29, 2020 Category: Microbiology Source Type: research

High-Throughput Characterization of Viral and Cellular Protein Expression Patterns During JC Polyomavirus Infection
Discussion The study of viral infections in vitro has provided innumerable advances to the field of virology. However, the lack of rapid and efficient screening tools has hindered research progress for some viruses, like JCPyV (Houff et al., 1983; Zu Rhein, 1983; Assetta and Atwood, 2017). To overcome this challenge, the development of high-throughput analyses is needed to help aid in the production of large data sets and generation of multiple lines of inquiry. Current methodologies for analyzing JCPyV infectivity predominantly rely on manual quantitation of infection by indirect immunodetection of viral proteins by epif...
Source: Frontiers in Microbiology - April 16, 2019 Category: Microbiology Source Type: research

Autophagy Is a Defense Mechanism Inhibiting Invasion and Inflammation During High-Virulent Haemophilus parasuis Infection in PK-15 Cells
In this study, we sought to investigate whether SH0165 (serovar 5, high-virulent strain) and HN0001 (serovar 6, non-virulent strain) infection induces autophagy and the specific role of autophagy in bacterial invasion and inflammation during H. parasuis infection. Moreover, we explored the mechanism underlying autophagy regulated inflammation through inflammatory signaling cascades during H. parasuis infection. This observation could provide useful information for further understanding the role of autophagy in H. parasuis infection and improve our knowledge of new strategies against this pathogen. Materials and Methods B...
Source: Frontiers in cellular and infection microbiology - April 15, 2019 Category: Microbiology Source Type: research

Therapeutic potentials of short interfering RNAs.
Abstract Short interfering RNA (siRNA) is one of the members of the family of RNA interference (RNAi). Coupled with the RNA-induced silencing complex (RISC), siRNA is able to trigger the cleavage of target RNAs which serve as a defensive system against pathogens. Meanwhile, siRNA in gene silencing opens a new avenue for the treatment of various diseases. SiRNA can effectively inhibit viral infection and replication and suppress tumorigenesis and various inflammation-associated diseases and cardiovascular diseases by inactivation of viral genes and downregulation of oncogene expression. Recently, endogenous siRNAs ...
Source: Applied Microbiology and Biotechnology - August 8, 2017 Category: Microbiology Authors: Tam C, Wong JH, Cheung RCF, Zuo T, Ng TB Tags: Appl Microbiol Biotechnol Source Type: research

Effect of small interfering RNA against Paracin 1.7 bacteriocin produced by Lactobacillus paracasei HD1‐7
In this study, the effect of siRNA on the expression of the most important proteins in the production of Paracin 1.7, sensor kinase (prcK) and response regulator (prcR), was investigated. SiRNA were designed against prcK and prcR, and qRT‐PCR was performed to examine the expression of prcK and prcR mRNA. The efficacy of siRNA was determined by comparing the level of antimicrobial activity of the strains. qRT‐PCR showed that siRNA‐K4 and siRNA‐K5 significantly inhibited the expression of prcK mRNA, and siRNA‐R4 and siRNA‐R6 significantly inhibited the expression of prcR mRNA. The proteins levels and antibacteria...
Source: Journal of Basic Microbiology - May 22, 2015 Category: Microbiology Authors: Jingping Ge, Yang Wang, Dongni Gao, Wenxiang Ping Tags: Full Paper Source Type: research

Long-term suppression of HIV-1C virus production in human peripheral blood mononuclear cells by LTR heterochromatization with a short double-stranded RNA
Conclusions We have identified a potent dsRNA that causes long-term suppression of HIV-1C virus production in vitro and ex vivo by heritable epigenetic modification at the targeted C-LTR region. This dsRNA has promising therapeutic potential in HIV-1C infection, the clade responsible for more than half of AIDS cases worldwide.
Source: Journal of Antimicrobial Chemotherapy - January 9, 2014 Category: Microbiology Authors: Singh, A., Palanichamy, J. K., Ramalingam, P., Kassab, M. A., Bhagat, M., Andrabi, R., Luthra, K., Sinha, S., Chattopadhyay, P. Tags: Original research Source Type: research

Prophylactic intranasal administration of lipid nanoparticle formulated siRNAs reduce SARS-CoV-2 and RSV lung infection
J Microbiol Immunol Infect. 2023 Mar 8:S1684-1182(23)00068-3. doi: 10.1016/j.jmii.2023.02.010. Online ahead of print.ABSTRACTRNA interference (RNAi) is an emerging and promising therapy for a wide range of respiratory viral infections. This highly specific suppression can be achieved by the introduction of short-interfering RNA (siRNA) into mammalian systems, resulting in the effective reduction of viral load. Unfortunately, this has been hindered by the lack of a good delivery system, especially via the intranasal (IN) route. Here, we have developed an IN siRNA encapsulated lipid nanoparticle (LNP) in vivo delivery system...
Source: Journal of Microbiology, Immunology, and Infection - March 18, 2023 Category: Microbiology Authors: Aroon Supramaniam Yaman Tayyar Daniel T W Clarke Gabrielle Kelly Dhruba Acharya Kevin V Morris Nigel A J McMillan Adi Idris Source Type: research