[Silencing the target rhoptry neck protein (RON) gene responsible for the invasion of Toxoplasma gondii with siRNA transfection].

[Silencing the target rhoptry neck protein (RON) gene responsible for the invasion of Toxoplasma gondii with siRNA transfection]. Mikrobiyol Bul. 2019 Jan;53(1):81-95 Authors: Yürük M, Aksoy T, Sivcan E, Nergiz H Abstract Toxoplasma gondii is a coccidian protozoan that causes toxoplasmosis is a common disease in Turkey as well as all over the world. It causes various clinical symptoms depending on the immune system status, age, or location of the disease. There is an organelle called the apical complex at the anterior end of the parasite. Rhoptry Neck Proteins (RONs), a component of this organelle, play a critical role in the formation of "moving junction" and parasitophorous vacuoles during host cell invasion. On the other hand, interfering RNA (iRNA) treatment options developed in recent years have emerged. With small iRNAs (siRNA) it is also possible to treat and control parasitic diseases, too. From here it is thought to use this method against toxoplasmosis. Within the scope of the project, it is aimed to silence RON1 gene the target invasion molecules of T.gondii with siRNA transfection. In the study, the negative control group constitute HeLa cells, the positive control group constitute HeLa cells infected with T.gondii tachyzoites and the experimental group constitute HeLa cells infected with T.gondii tachyzoites after siRNA transfection were used. Samples were collected in each study group at 30 seconds, 1 minute, 5 minute...
Source: Mikrobiyoloji Bulteni - Category: Microbiology Tags: Mikrobiyol Bul Source Type: research