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Total 92 results found since Jan 2013.

Comparative Study of the Effects of Ferrochelatase ‐siRNA Transfection Mediated by Ultrasound Microbubbles and Polyethyleneimine in Combination with Low‐dose ALA to Enhance PpIX Accumulation in Human Endometrial Cancer Xenograft Nude Mice Models
Red fluorescence caused by the accumulation of PpIX in endometrial cancer xenografts in nude mice after high ‐dose ALA injection could be detected easily byin  vivo animal imaging system, but low ‐dose ALA (1.0 mg kg−1) was injected into xenografts, there was no red fluorescence of tumor. However, knockdown of FECH in nude mice was performed by transfection with FECH ‐siRNA mediated by PEI and ultrasound microbubbles alone or in combination, then 1.0 mg kg−1 ALA was injected, apparent red fluorescence of the xenografts was observed, and the fluorescence intensity was PEI+ ultrasound microbubbles  >  PEI...
Source: Photochemistry and Photobiology - February 11, 2019 Category: Science Authors: Xian Zhang, Longfei Chen, Lvfen Gao, Xuesong Gao, Nan Li, Yuwei Song, Xinke Huang, Shaoqiang Lin, Xiaoyu Wang Tags: Research Article Source Type: research

Low-intensity pulsed ultrasound attenuates cardiac inflammation of CVB3-induced viral myocarditis via regulation of caveolin-1 and MAPK pathways.
Abstract The aggressive immunological activity elicited by acute viral myocarditis contributes to a large amount of cardiomyocytes loss and poor prognosis of patients in clinic. Low-intensity pulsed ultrasound (LIPUS), which is an effective treatment modality for osteoarthropathy, has been recently illustrated regulating the overactive inflammatory response in various diseases. Here, we aimed to investigate whether LIPUS could attenuate coxsackievirus B3 (CVB3) infection-induced injury by coordinating the inflammatory response. Male BALB/c mice were inoculated intraperitoneally with CVB3 to establish the model of ...
Source: J Cell Mol Med - December 27, 2018 Category: Molecular Biology Authors: Zheng C, Wu SM, Lian H, Lin YZ, Zhuang R, Thapa S, Chen QZ, Chen YF, Lin JF Tags: J Cell Mol Med Source Type: research

Comparative Study of the Effects of FECH ‐siRNA Transfection Mediated by Ultrasound Microbubbles and PEI in Combination with Low‐dose ALA to Enhance PpIX Accumulation in Human Endometrial Cancer Xenograft Nude Mice Models
This article is protected by copyright. All rights reserved.
Source: Photochemistry and Photobiology - December 24, 2018 Category: Science Authors: Xian Zhang, Longfei Chen, Lvfen Gao, Xuesong Gao, Nan Li, Yuwei Song, Xinke Huang, Shaoqiang Lin, Xiaoyu Wang Tags: Research Article Source Type: research

Nucleic Acid Delivery System by the Combination of Lipid bubbles and Ultrasound.
CONCLUSION: Ultrasound technology is widely used in clinical settings not only for diagnosis but also for therapy. Ultrasonic devices are being actively developed. Computer-controlled ultrasound systems can provide precise exposure to the target site. The combination of precise ultrasound exposure and LBs might be useful for target site-specific nucleic acids delivery, and holds potential to be developed into a beneficial therapeutic and diagnostic system for various diseases. PMID: 30084324 [PubMed - as supplied by publisher]
Source: Current Pharmaceutical Design - August 7, 2018 Category: Drugs & Pharmacology Authors: Endo-Takahashi Y, Maruyama K, Negishi Y Tags: Curr Pharm Des Source Type: research

Ultrasound assisted gene and photodynamic synergistic therapy with multifunctional FOXA1-siRNA loaded porphyrin microbubbles for enhancing therapeutic efficacy for breast cancer.
Abstract To improve the non-invasive therapeutic efficacy for ER positive breast cancer (ER+ BC), we fabricated a multifunctional FOXA1 loaded porphyrin microbubble to combine photodynamic therapy (PDT) and gene therapy of FOXA1 knockdown (KD) with ultrasound targeted microbubble destruction (UTMD) technology under the guidance of contrast enhanced ultrasound (CEUS). Cationic porphyrin microbubbles (CpMBs) were firstly fabricated from a porphyrin grafted lipid with two cationic amino groups (PGL-NH2) and fluorocarbon inert gas of C3F8. Porphyrin group in the CpMBs monolayer could be used as a photosensitizer for ...
Source: Biomaterials - May 3, 2018 Category: Materials Science Authors: Zhao R, Liang X, Zhao B, Chen M, Liu R, Sun S, Yue X, Wang S Tags: Biomaterials Source Type: research

Plasmas, membranes, and transport across the membranes
Publication date: February 2018 Source:Clinical Plasma Medicine, Volume 9, Supplement Author(s): Lluis M. Mir Physical agents, like cold plasma, may affect the cells at various levels, amongst which the cell membrane. Cell or “plasma” membrane is a vital “organ” for the cell. Indeed if cell membrane integrity is affected, transports from cell outside to cell inside and from cell inside to cell outside can no longer be regulated [1]. It is essential for the cell physiology and survival to keep fully regulated the transport across the cell membrane of ions (Na+, Ca++, K+, Cl-), of small molecules (sugars, ATP, amino...
Source: Clinical Plasma Medicine - April 10, 2018 Category: Research Source Type: research

Ultrasound-mediated nanobubble destruction (UMND) facilitates the delivery of A10-3.2 aptamer targeted and siRNA-loaded cationic nanobubbles for therapy of prostate cancer.
In this study, we synthesized a promising anti-tumor targeted FoxM1 siRNA-loaded cationic nanobubbles (CNBs) conjugated with an A10-3.2 aptamer (siFoxM1-Apt-CNBs), which demonstrate high specificity when binding to prostate-specific membrane antigen (PSMA) positive LNCaP cells. Uniform nanoscaled siFoxM1-Apt-CNBs were developed using a thin-film hydration sonication, carbodiimide chemistry approaches, and electrostatic adsorption methods. Fluorescence imaging as well as flow cytometry evidenced the fact that the siFoxM1-Apt-CNBs were productively developed and that they specifically bound to PSMA-positive LNCaP cells. siFo...
Source: Drug Delivery - January 11, 2018 Category: Drugs & Pharmacology Tags: Drug Deliv Source Type: research

Cationic gas-filled microbubbles for ultrasound-based nucleic acids delivery.
Abstract The use of ultrasound has gained great interest for nucleic acids delivery. Ultrasound can reach deep tissues in non-invasive manner. The process of sonoporation is based on the use of low frequency ultrasound combined with gas-filled microbubbles allowing an improved delivery of molecules including nucleic acids in the insonified tissue. For in vivo gene transfer, the engineering of cationic microbubbles is essential for creating strong electrostatic interactions between microbubbles and nucleic acids leading to their protection against nucleases degradation and high concentration within the target tissu...
Source: Bioscience Reports - November 27, 2017 Category: Biomedical Science Authors: Delalande A, Bastié C, Pigeon L, Manta S, Lebertre M, Mignet N, Midoux P, Pichon C Tags: Biosci Rep Source Type: research

Enhanced delivery of biodegradable mPEG-PLGA-PLL nanoparticles loading Cy3-labelled PDGF-BB siRNA by UTMD to rat retina.
Abstract We investigated the efficacy and safety of ultrasound (US)-targeted microbubble (MB) destruction (UTMD)-enhanced delivery of monomethoxypoly(ethylene glycol)-poly(lactic-co-glycolic acid)-poly-L-lysine (mPEG-PLGA-PLL) nanoparticles (NPs) loading Cy3-labelled platelet-derived growth factor BB (PDGF-BB) siRNA to rat retina in vivo. Eighty Wistar rats were divided into five groups (G). The right eyes, respectively, received an intravitreal injection as follows: normal saline (NS) (G1), NPs and NS (G2), NPs and MBs (G3), NPs and NS (G4) and NPs and MBs (G5). In G4 and G5, the eyes were exposed to US for 5 min...
Source: Journal of Biosciences - June 1, 2017 Category: Biomedical Science Authors: DU J, Sun Y, Li FH, DU LF, Duan YR Tags: J Biosci Source Type: research

Nucleic acids-based nanotherapeutics crossing the blood brain barrier.
Abstract The restless endeavors revealing the molecular pathways underlying many neurodegenerative diseases and brain tumors have paved the way for the introduction of the selective exogenous gene-based therapeutics. The implicated active biomolecules encompass mainly negatively-charged nucleic acids ranging from DNA, mRNA, non-coding RNAs (small-interfering RNA, siRNA, and microRNA, miRNA), to antisense oligonucleotides. They selectively interfere with the genes translational and/or transcriptional processes. Although many reviews previously addressed brain targeting, a thorough correlation between the molecular ...
Source: Current Gene Therapy - May 10, 2017 Category: Genetics & Stem Cells Authors: Nafee N, Gouda N Tags: Curr Gene Ther Source Type: research

Valproate induced hepatic steatosis by enhanced fatty acid uptake and triglyceride synthesis.
Abstract Steatosis is the characteristic type of VPA-induced hepatotoxicity and may result in life-threatening hepatic lesion. Approximately 61% of patients treated with VPA have been diagnosed with hepatic steatosis through ultrasound examination. However, the mechanisms underlying VPA-induced intracellular fat accumulation are not yet fully understood. Here we demonstrated the involvement of fatty acid uptake and lipogenesis in VPA-induced hepatic steatosis in vitro and in vivo by using quantitative real-time PCR (qRT-PCR) analysis, western blotting analysis, fatty acid uptake assays, Nile Red staining assays, a...
Source: Toxicology and Applied Pharmacology - March 30, 2017 Category: Toxicology Authors: Bai X, Hong W, Cai P, Chen Y, Xu C, Cao D, Yu W, Zhao Z, Huang M, Jin J Tags: Toxicol Appl Pharmacol Source Type: research

Exogenous H2S regulates endoplasmic reticulum-mitochondria cross-talk to inhibit apoptotic pathways in STZ-induced type I diabetes
This study investigated cardiac function, ultrastructural changes in the ER and mitochondria, apoptotic rate using TUNEL, and the expression of ER stress-associated proteins and mitochondrial apoptotic proteins in cardiac tissues in STZ-induced type I diabetic rats treated with or without NaHS (donor of H2S). Mitochondria of cardiac tissues were isolated, and MPTP opening and cytochrome c (cyt C) and Mfn-2 expression were also detected. Our data showed that hyperglycemia decreased the cardiac function by ultrasound cardiogram, and the administration of exogenous H2S ameliorated these changes. We demonstrated that the expre...
Source: AJP: Endocrinology and Metabolism - March 1, 2017 Category: Endocrinology Authors: Yang, F., Yu, X., Li, T., Wu, J., Zhao, Y., Liu, J., Sun, A., Dong, S., Wu, J., Zhong, X., Xu, C., Lu, F., Zhang, W. Tags: Research Article Source Type: research

LIPUS suppressed LPS ‐induced IL‐1α through the inhibition of NF‐κB nuclear translocation via AT1‐PLCβ pathway in MC3T3‐E1
This article is protected by copyright. All rights reserved
Source: Journal of Cellular Physiology - January 6, 2017 Category: Cytology Authors: Mayu Nagao, Natsuko Tanabe, Soichiro Manaka, Masako Naito, Kumiko Nakai, Tadahiro Takayama, Takayuki Kawato, Go Torigoe, Jumpei Sekino, Naoya Tsukune, Masao Maeno, Naoto Suzuki, Shuichi Sato Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

Exogenous H2S regulates endoplasmic reticulum-mitochondria cross-talk to inhibit apoptotic pathways in STZ-induced type I diabetes.
CONCLUSION: Hyperglycemia stimulated ER interactions and mitochondrial apoptotic pathways, which were inhibited by exogenous H2S treatment through the regulation of Mfn-2 expression. PMID: 27998959 [PubMed - as supplied by publisher]
Source: Am J Physiol Endocri... - December 19, 2016 Category: Endocrinology Authors: Yang F, Yu X, Li T, Wu J, Zhao Y, Sun A, Dong S, Wu J, Zhong X, Xu C, Lu F, Zhang W Tags: Am J Physiol Endocrinol Metab Source Type: research

Gene delivery systems by the combination of lipid bubbles and ultrasound.
Authors: Negishi Y, Endo-Takahashi Y, Maruyama K Abstract Gene therapy is promising for the treatment of many diseases including cancers and genetic diseases. From the viewpoint of safety, ultrasound (US)-mediated gene delivery with nano/ microbubbles was recently developed as a novel non-viral vector system. US-mediated gene delivery using nano/microbubbles are able to produce transient changes in the permeability of the cell membrane after US-induced cavitation while reducing cellular damage and enables the tissue-specific or the site-specific intracellular delivery of gene both in vitro and in vivo. We have rece...
Source: Drug Discoveries and Therapeutics - November 1, 2016 Category: Drugs & Pharmacology Tags: Drug Discov Ther Source Type: research