Ultrasound-mediated nanobubble destruction (UMND) facilitates the delivery of A10-3.2 aptamer targeted and siRNA-loaded cationic nanobubbles for therapy of prostate cancer.
In this study, we synthesized a promising anti-tumor targeted FoxM1 siRNA-loaded cationic nanobubbles (CNBs) conjugated with an A10-3.2 aptamer (siFoxM1-Apt-CNBs), which demonstrate high specificity when binding to prostate-specific membrane antigen (PSMA) positive LNCaP cells. Uniform nanoscaled siFoxM1-Apt-CNBs were developed using a thin-film hydration sonication, carbodiimide chemistry approaches, and electrostatic adsorption methods. Fluorescence imaging as well as flow cytometry evidenced the fact that the siFoxM1-Apt-CNBs were productively developed and that they specifically bound to PSMA-positive LNCaP cells. siFoxM1-Apt-CNBs combined with ultrasound-mediated nanobubble destruction (UMND) significantly improved transfection efficiency, cell apoptosis, and cell cycle arrest in vitro while reducing FoxM1 expression. In vivo xenografts tumors in nude-mouse model results showed that siFoxM1-Apt-CNBs combined with UMND led to significant inhibition of tumor growth and prolonged the survival of the mice, with low toxicity, an obvious reduction in FoxM1 expression, and a higher apoptosis index. Our study suggests that siFoxM1-Apt-CNBs combined with UMND might be a promising targeted gene delivery strategy for therapy of prostate cancer. PMID: 29313393 [PubMed - in process]
Authors: De Luca S, Fiori C, Bollito E, Garrou D, Aimar R, Cattaneo G, De Cillis S, Manfredi M, Tota D, Massa F, Passera R, Porpiglia F Abstract BACKGROUND: To evaluate if multiparametric magnetic resonance (mpMRI)- transrectal ultrasound (TRUS) fusion targeted biopsy (TBx) versus untargeted standard biopsy (SBx) may decrease the rate of pathological upgrading of Gleason score (GS) 3+4 prostate cancer (PCa) at radical prostatectomy (RP). We also evaluated the impact of percent pattern 4 and cribriform glands at biopsy in the risk of GS 3 + 4 = 7 upgrading. METHODS: 301 patients with GS 3+4 PCa on biopsy (159 SB...
Paul Sayer, 64, from Southend-on-Sea in Essex, was diagnosed with cancer in his 60s. He had high-intensity focused ultrasound treatment in July 2018 and suffered no complications afterwards.
CONCLUSIONS: The rate of overall complications after TRUS was low. The most common complications requiring emergency attendances and hospitalisations were gross haematuria and acute urinary retention, respectively. Prostate volume>48 cc increased the risk of post-biopsy urinary retention. PMID: 31601774 [PubMed - as supplied by publisher]
Publication date: October 2019Source: European Urology Supplements, Volume 18, Issue 9Author(s): D. Maffei, M. Paciotti, M. Lazzeri, L. Zhang, N. Frego, P. Colombo, P. Casale, R. Hurle, A. Saita, N. Buffi, G. Guazzoni, G. Lughezzani
Publication date: October 2019Source: European Urology Supplements, Volume 18, Issue 9Author(s): F. Chessa, A. Ercolino, C. Gaudiano, D. Giusti, L. Bianchi, M. Borghesi, C. Pultrone, E. Marcelli, L. Lodigiani, R. Schiavina, E. Brunocilla
Publication date: October 2019Source: European Urology Supplements, Volume 18, Issue 9Author(s): G. Di Cosmo, A. Russo, L. Lopez, R. Gaston
CONCLUSIONS: Prostate biopsies may lead to a tumor-associated release of CTCs into the blood circulation. Larger confirmatory trials with longer follow-up periods are required before any change in clinical practice can be recommended. PMID: 31601564 [PubMed - as supplied by publisher]
ConclusionsIn this paper, technical feasibility of multiparametric machine learning to improve upon single US modalities for the localization of PCa has been demonstrated. Extended datasets for training and testing may establish the clinical value of automatic multiparametric US classification in the early diagnosis of PCa.Key Points• Combination of B-mode ultrasound, shear-wave elastography, and contrast ultrasound radiomics through machine learning is technically feasible.• Multiparametric ultrasound demonstrated a higher prostate cancer localization ability than single ultrasound modalities.• Computer-aid...
CONCLUSIONS: Our study shows that the use of Sonovue after HIFU FT was safe. Patients present a significant proportion of failure after HIFU FT but with good functional outcomes and without incidence of severe complications. PMID: 31579041 [PubMed - in process]
CONCLUSIONS: Although this is a preliminary series, MUS is presented as an attractive imaging technique, cost-effective, easy to learn and with high efficacy in image- guided prostate biopsy. Overall PCa detection rate increases over conventional ultrasound; and offers high sensitivity and NPV in csPCa detection over mpMRI but with lower specificity. PMID: 31579039 [PubMed - in process]