Heat resistance and presence of genes encoding staphylococcal enterotoxins evaluated by multiplex-PCR of Staphylococcus aureus isolated from pasteurized camel milk.
Abstract Milk pasteurization eliminates vegetative pathogenic microorganisms and reduces microorganisms associated with spoilage. Camel milk is a well-accepted, traditionally consumed food in Arab countries. The present study aimed to investigate the microflora of pasteurized camel milk sold in Riyadh City, Saudi Arabia. The heat resistance of the microflora was tested in culture medium and lab-sterilized milk, and its composition was verified by multiplex polymerase chain reaction (PCR) using specific primers. Further verification was performed by using separate specific primers. The identified strain survived he...
Source: Bioscience Reports - November 16, 2019 Category: Biomedical Science Authors: Yehia HM, Ismail EA, Hassan ZK, Al-Masoud AH, Al-Dagal MM Tags: Biosci Rep Source Type: research

The association of AGO1 (rs595961G > A, rs636832A > G) and AGO2 (rs11996715C > A, rs2292779C > , rs4961280C > A) polymorphisms and risk of recurrent implantation failure.
The association of AGO1 (rs595961G>A, rs636832A>G) and AGO2 (rs11996715C>A, rs2292779C>, rs4961280C>A) polymorphisms and risk of recurrent implantation failure. Biosci Rep. 2019 Nov 14;: Authors: Ryu CS, Kim YR, Kim JO, An HJ, Cho SH, Ahn EH, Kim JH, Lee WS, Kim NK Abstract Recurrent implantation failure (RIF) is a common reproductive clinical condition treated by fertility specialists at in vitro fertilization (IVF) clinics. Several factors affect embryo implantation including the age of the female, the quality of embryos and the sperm, genetics, immunologic factors. Here, we investiga...
Source: Bioscience Reports - November 14, 2019 Category: Biomedical Science Authors: Ryu CS, Kim YR, Kim JO, An HJ, Cho SH, Ahn EH, Kim JH, Lee WS, Kim NK Tags: Biosci Rep Source Type: research

The clinical efficacy and safety of kanglaite adjuvant therapy in the treatment of advanced hepatocellular carcinoma: a PRISMA-compliant meta-analysis.
Abstract Kanglaite, a type of Chinese medicine preparation, is considered a promising complementary therapy option for advanced hepatocellular carcinoma (HCC). Although an analysis of the published literature has been performed, the exact effects and safety have yet to be systematically investigated. Therefore, we conducted a wide-ranging online search of electronic databases to provide systematic conclusions; data from 31 trials with 2,315 HCC patients were included. The results indicated that compared with conventional treatment (CT) alone, the combination of kanglaite with CT markedly prolonged patient...
Source: Bioscience Reports - November 12, 2019 Category: Biomedical Science Authors: Liu J, Liu X, Ma J, Li K, Xu C Tags: Biosci Rep Source Type: research

SP1-DLEU1-miR-4429 feedback loop promotes cell proliferative and anti-apoptotic abilities in human glioblastoma.
Abstract Mounting studies have revealed that long non-coding RNA (lncRNA) deleted in lymphocytic leukemia 1 (DLEU1) positively regulated the initiation and development of various human malignant tumors. Nevertheless, the function and mechanism of DLEU1 in human glioblastoma (GBM) remain elusive and ill-defined. The current study was designed to highlight the functional role and disclose the underlying molecular mechanism by which DLEU1 regulated GBM development. We found that DLEU1 was upregulated in GBM and DLEU1 knockdown significantly inhibited GBM cell proliferation and induced apoptosis. As predicted by bioin...
Source: Bioscience Reports - November 12, 2019 Category: Biomedical Science Authors: Liu X, Chen R, Liu L Tags: Biosci Rep Source Type: research

Comprehensive assessment of the association between XPC rs2228000 and cancer susceptibility based on 26,835 cancer cases and 37,069 controls.
Conclusions The TT genotype of XPC rs2228000 may be linked to an increased risk of bladder and breast cancer, whereas the CT genotype is likely to be associated with reduced susceptibility to gastric cancer in the Chinese population. PMID: 31710080 [PubMed - as supplied by publisher] (Source: Bioscience Reports)
Source: Bioscience Reports - November 11, 2019 Category: Biomedical Science Authors: Dai Y, Song Z, Zhang J, Gao W Tags: Biosci Rep Source Type: research

Endoplasmic Reticulum Ca2+ Release Causes Rieske Iron-Sulfur Protein-mediated Mitochondrial ROS Generation in Pulmonary Artery Smooth Muscle Cells.
Abstract Mitochondrial reactive oxygen species (ROS) cause Ca2+ release from the Endoplasmic reticulum (ER) via ryanodine receptors (RyRs) in pulmonary artery smooth muscle cells (PASMCs), playing an essential role in hypoxic pulmonary vasoconstriction (HPV). Here we tested a novel hypothesis that hypoxia-induced RyR-mediated Ca2+ release may, in turn, promote mitochondrial ROS generation contributing to hypoxic cellular responses in PASMCs. Our data reveal that application of caffeine to elevate intracellular Ca2+ concentration ([Ca2+]i) by activating RyRs results in a significant increase in ROS production in cy...
Source: Bioscience Reports - November 11, 2019 Category: Biomedical Science Authors: Dong D, Hao Q, Zhang P, Wang T, Han F, Liang X, Fei Z Tags: Biosci Rep Source Type: research

Long non-coding RNA TCONS_00000200 as a non-invasive biomarker in patients with intracranial aneurysm.
Abstract We performed long non-coding RNA (lncRNA) microarray assay to identify lncRNAs with differential expression between patients with intracranial aneurysm (IA) and healthy control individuals to evaluate their potential use as biomarkers of IA. Arraystar Human lncRNA Microarray v3.0 was performed to identify differentially expressed lncRNAs and mRNAs in plasma samples (4 ml). lncRNAs with the most pronounced differential expression were used to select gene markers, and results were validated by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Plasma levels of TCONS_00000200 (fold chang...
Source: Bioscience Reports - November 11, 2019 Category: Biomedical Science Authors: Wu C, Song H, Wang Y, Gao L, Cai Y, Cheng Q, Chen Y, Zheng Z, Liao Y, Lin J, Xie B, Cai W, Li S, Liao L, Yan X Tags: Biosci Rep Source Type: research

-196 to -174del, rs4696480, rs3804099 polymorphisms of Toll-like receptor 2 gene impact the susceptibility of cancers: evidence from 37053 subjects.
Abstract Relationship between Toll-like receptor-2 (TLR2) and cancer risk has been illustrated in some studies, but their conclusions are inconsistent. Therefore, we designed this meta-analysis to explore a more accurate conclusion of whether TLR2 affect cancer risks. Articles were retrieved from various literature databases according to the criteria. We use STATA to calculate the odds ratio (OR) and 95% confidence interval (CI) to evaluate the relationship between certain polymorphism of TLR2 and cancer risk. Finally, 47 case-control studies met the criteria, comprise of 15851 cases and 21182 controls. In the ove...
Source: Bioscience Reports - November 11, 2019 Category: Biomedical Science Authors: Gao SL, Chen YD, Yue C, Chen J, Zhang LF, Wang SM, Zuo L Tags: Biosci Rep Source Type: research

Koebner Phenomenon leading to formation of new psoriatic lesions: Evidences and mechanisms.
Abstract Koebner phenomenon refers to the emergence of new psoriatic lesions in the healthy skin regions following an injury/trauma to psoriatic patients. The occurrence of psoriatic lesions at unusual areas of the body regions such as on penis, around eyes and on keloids suggests that the Koebner phenomenon may be responsible for these lesions. A number of agents/triggers have been reported to induce the development of new psoriatic lesions in healthy skin areas and these include, tattooing skin, radiations, skin incision, viral infections, and striae, etc. The different mechanisms that contribute in inducin...
Source: Bioscience Reports - November 11, 2019 Category: Biomedical Science Authors: Ji YZ, Liu SR Tags: Biosci Rep Source Type: research

Sevoflurane modulates AQPs (1,5) expression and endoplasmic reticulum stress in mice lung with allergic airway inflammation.
This study aimed to determine the role of sevoflurane in AQPs (AQP1,3,4,5) expression in mice with allergic airway inflammation and the probable mechanism. The increased number of inflammatory cells infiltrating the lung tissue, and the elevated levels of tumor necrosis factor-α and interleukin-13 were all decreased after sevoflurane treatment (all P < 0.05). Meanwhile, mRNA levels of AQP1 and AQP5 but not AQP3 and AQP4 were decreased in OVA-induced allergic mice lung. Both the decreased mRNA expression and protein levels of AQP1 and AQP5 in allergic lung tissues were reversed by sevoflurane treatment. Further...
Source: Bioscience Reports - November 11, 2019 Category: Biomedical Science Authors: Lv CM, Wu HM, Wu L, Xu GH, Yang ZL, Shen QY Tags: Biosci Rep Source Type: research

Response   to comments on "Nomograms based on inflammatory biomarkers for predicting tumor grade and micro-vascular invasion in stage I/IIhepatocellular carcinoma ".
Response  to comments on "Nomograms based on inflammatory biomarkers for predicting tumor grade and micro-vascular invasion in stage I/IIhepatocellular carcinoma ". Biosci Rep. 2019 Nov 08;: Authors: Chen BC, Li P, Huang W, Wang F, Ke YF, Gao L, Shi KQ, Zhou MT Abstract We appreciate to receive commentary from Dr Guangtong Deng and Dr Liang Xiao to our article "Nomograms based on inflammatory biomarkers for predicting tumor grade and micro-vascular invasion in stage I/II hepatocellular carcinoma". First, NLR and dNLR are two different parameters. Some studi...
Source: Bioscience Reports - November 8, 2019 Category: Biomedical Science Authors: Chen BC, Li P, Huang W, Wang F, Ke YF, Gao L, Shi KQ, Zhou MT Tags: Biosci Rep Source Type: research

MiR-942 regulates the function of breast cancer cell by targeting FOXA2.
Abstract MicroRNA (MiR)-942 regulates the development of a variety of tumors, however, its function in breast cancer (BCa) has been less reported. Therefore, this study investigated the regulatory effects of miR-942 on BCa cells. The expression of miR-942 in whole blood samples and BCa cell lines was detected by quantitative real-time (qRT)-PCR. Direct target gene for miR-942 was confirmed by dual-luciferase reporter assay. FOXA2 expression in adjacent tissues was detected by qRT-PCR. The effects of miR-942, or miR-942 with FOXA2, on the cell viability, proliferation, apoptosis, migration and invasion of BCa cells...
Source: Bioscience Reports - November 8, 2019 Category: Biomedical Science Authors: Zhang J, Zhang Z, Sun J, Ma Q, Zhao W, Chen X, Qiao H Tags: Biosci Rep Source Type: research

Salidroside mitigates skeletal muscle atrophy in rats with cigarette smoke-induced COPD by up-regulating myogenin and down-regulating myostatin expression.
CONCLUSION: Salidroside mitigates the long-term cigarette smoking-induced emphysema and skeletal muscle atrophy in rats by inhibiting oxidative stress and inflammatory responses and regulating muscle-specific transcription factor expression. PMID: 31702007 [PubMed - as supplied by publisher] (Source: Bioscience Reports)
Source: Bioscience Reports - November 8, 2019 Category: Biomedical Science Authors: Zhang D, Cao L, Wang Z, Feng H, Cai X, Xu M, Li M, Yu N, Yin Y, Wang W, Kang J Tags: Biosci Rep Source Type: research

Emerging roles of the long non-coding RNA 01296/microRNA-143-3p/MSI2 axis in development of thyroid cancer.
Abstract Thyroid cancer (TC) is an endocrine malignancy with rising incidence. Long non-coding RNAs (lncRNAs) can serve as diagnostic and prognostic biomarkers for TC. Thus, we studied roles of LINC01296 in TC progression. Initially, the Gene Expression Omnibus database was used to detect the differentially expressed genes in human TC samples and the potential mechanism. Expression of LINC01296 and miR-143-3p in TC tissues and cells was measured. The transfection of TC cells was conducted with si-LINC01296, si-MSI2, mimic or inhibitor of miR-143-3p to determine their effects on TC cell proliferation, migration, in...
Source: Bioscience Reports - November 6, 2019 Category: Biomedical Science Authors: Wang ZL, Wang C, Liu W, Ai ZL Tags: Biosci Rep Source Type: research

Long non-coding RNA ROR1-AS1 incudes tumorigenesis of colorectal cancer by affecting Wnt/ β-catenin signaling pathway.
Long non-coding RNA ROR1-AS1 incudes tumorigenesis of colorectal cancer by affecting Wnt/β-catenin signaling pathway. Biosci Rep. 2019 Nov 06;: Authors: Wang W, Zheng W, Zhang L, Li K Abstract Recent studies have discovered that long noncoding RNAs (lncRNAs) play an important role in malignant tumors. In this research, lncRNA ROR1-AS1 was selected to identify how it affects the development of colorectal cancer (CRC). ROR1-AS1 expression was detected by RT-qPCR in CRC tissue samples. ROR1-AS1 expression level and patients' overall survival time were analyzed. Functional experiments were conducted ...
Source: Bioscience Reports - November 6, 2019 Category: Biomedical Science Authors: Wang W, Zheng W, Zhang L, Li K Tags: Biosci Rep Source Type: research

Catestatin prevents endothelial inflammation and promotes thrombus resolution in acute pulmonary embolism in mice.
This study aims to investigate the effects of CTS on thrombus formation that may inhibit the development of pulmonary embolism and its potential pathway. Acute pulmonary embolism (APE) model was developed as an in vivo model. The effects of CTS on mice with APE were examined. Human pulmonary artery endothelial cells (HPAECs) were pretreated with CTS before thrombin stimulation, and endothelial inflammation and underlying mechanisms were evaluated in vitro. That plasma CTS level was decreased in APE mice, while the number of platelets was significantly increased. The decreased circulating CTS level negatively associated wit...
Source: Bioscience Reports - November 4, 2019 Category: Biomedical Science Authors: Chen H, Liu D, Ge L, Wang T, Ma Z, Han Y, Duan Y, Xu X, Liu W, Yuan J, Liu J, Li R, Du R Tags: Biosci Rep Source Type: research

lncRNA GHET1 promotes cervical cancer progression through regulating AKT/mTOR and Wnt/ β-catenin signaling pathways.
In conclusion, we revealed that down-regulation of GHET1 suppresses cervical cancer progression through regulating AKT/mTOR and Wnt/β-catenin signaling pathways, indicating GHET1 as a promising molecular biomarker for CC treatment improvement. PMID: 31682716 [PubMed - as supplied by publisher] (Source: Bioscience Reports)
Source: Bioscience Reports - November 4, 2019 Category: Biomedical Science Authors: Liu Z, Luo S, Wu M, Huang C, Shi H, Song X Tags: Biosci Rep Source Type: research

The association between IL-17 gene variants and risk of colorectal cancer in a Chinese population: a case-control study.
In conclusion, our data indicate that IL-17A rs2275913 polymorphism but not IL-17F rs763780 polymorphism contributes to increased risk for CRC patients in this Chinese population. PMID: 31682719 [PubMed - as supplied by publisher] (Source: Bioscience Reports)
Source: Bioscience Reports - November 4, 2019 Category: Biomedical Science Authors: Feng H, Ying R, Chai T, Chen H, Ju H Tags: Biosci Rep Source Type: research

Evaluation of Different b-Values in DWI and 1H MRS for Pancreatic cancer and pancreatitis: A rabbit model.
This study determined that the effective differential diagnosis of pancreatic cancer and pancreatitis was determined at low b values (333s/mm2) when performed DWI inspection in rabbit models. PMID: 31670373 [PubMed - as supplied by publisher] (Source: Bioscience Reports)
Source: Bioscience Reports - October 31, 2019 Category: Biomedical Science Authors: Hao L, Liu L, Meng X, Yu G, Li E Tags: Biosci Rep Source Type: research

Homocysteine and Psoriasis.
Abstract Psoriasis is caused by a complex interplay among the immune system, genetic background, autoantigens, and environmental factors. Recent studies have demonstrated that patients with psoriasis have a significantly higher serum homocysteine (Hcy) level and a higher prevalence of hyperhomocysteinaemia (HHcy). Insufficiency of folic acid and vitamin B12 can be a cause of HHcy in psoriasis. Hcy may promote the immuno-inflammatory process in the pathogenesis of psoriasis by activating Th1 and Th17 cells and neutrophils, while suppressing regulatory T cells Moreover, Hcy can drive the immuno-inflammatory process ...
Source: Bioscience Reports - October 31, 2019 Category: Biomedical Science Authors: Lin X, Meng X, Song Z Tags: Biosci Rep Source Type: research

Metabolomics study of fasudil on cisplatin-induced kidney injury.
Abstract Fasudil is a derivative of 5-isoquinoline sulfonamide, which is a Rho kinase inhibitor, a wide range of pharmacological effects. Fasudil has been shown to attenuate kidney injury caused by certain substances. In the present study, metabolomic analysis of mouse kidney tissues ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry was used to determine the metabolomic changes in cisplatin-induced kidney injury and the fasudil-induced attenuation of cisplatin-induced kidney injury. Metabolomic profiling of kidney tissues revealed significant differences in metabolites between the...
Source: Bioscience Reports - October 31, 2019 Category: Biomedical Science Authors: Xia M, Lai XL, Wu KW, Zhou PY, Li L, Guo ZY, Xu S Tags: Biosci Rep Source Type: research

High Glucose Provokes Microvesicles Generation from Glomerular Podocytes via NOX4/ROS Pathway.
Abstract Microvesicles (MVs) were involved in the pathogenesis of many diseases, such as cardiovascular diseases and diabetes. Oxidative stress played a key role in the development and progression of diabetic nephropathy. Our aim of this study was to investigate whether high glucose could provoke microvesicles generation from podocytes and its potential mechanism. Mouse podocyte clone 5 (MPC-5) were stimulated by high glucose. The intracellular reactive oxygen species (ROS) of podocytes were measured by fluorescence microscopy with the probe of CM-H2DCFDA and MitoSOXTM. Antioxidants N-Acetyl-L-cysteine (NAC) ...
Source: Bioscience Reports - October 30, 2019 Category: Biomedical Science Authors: Li M, Zhang T, Wu X, Chen Y, Sun L Tags: Biosci Rep Source Type: research

MicroRNA-451 inhibits inflammation and proliferation of glomerular mesangial cells through down-regulating PSMD11 and NF- κB p65.
MicroRNA-451 inhibits inflammation and proliferation of glomerular mesangial cells through down-regulating PSMD11 and NF-κB p65. Biosci Rep. 2019 Oct 30;39(10): Authors: Wei H, Li J, Li Y, Song J Abstract The present study aimed to investigate the regulatory roles of microRNA-451 (miR-451) on the inflammation and proliferation of glomerular mesangial cells (GMCs) under high-glucose condition, and reveal the potential mechanisms related to 26S proteasome non-ATPase regulatory subunit 11 (PSMD11) and nuclear factor-κ B (NF-κB) signaling. The interaction between PSMD11 and miR-451 was i...
Source: Bioscience Reports - October 29, 2019 Category: Biomedical Science Authors: Wei H, Li J, Li Y, Song J Tags: Biosci Rep Source Type: research

Berberine improves insulin resistance in adipocyte models by regulating the methylation of hypoxia-inducible factor-3 α.
Berberine improves insulin resistance in adipocyte models by regulating the methylation of hypoxia-inducible factor-3α. Biosci Rep. 2019 Oct 30;39(10): Authors: Wang Y, Gong W, Lv S, Qu H, He Y Abstract Methylation of hypoxia-inducible factor-3α (HIF3A) was previously demonstrated to be highly associated with insulin resistance (IR) in patients with gestational diabetes mellitus (GDM). We aimed to study the therapeutic effects of Berberine (BBR) on GDM and the possible mechanisms. The expressions and methylated states of HIF3A in pregnant women with GDM were compared with that in healthy c...
Source: Bioscience Reports - October 29, 2019 Category: Biomedical Science Authors: Wang Y, Gong W, Lv S, Qu H, He Y Tags: Biosci Rep Source Type: research

Wild-type menin is rapidly degraded via the ubiquitin-proteasome pathway in a rat insulinoma cell line.
Abstract Menin is encoded by multiple endocrine neoplasia type 1 (MEN1) gene, the germ line mutations of which are the main cause of pancreatic neuroendocrine tumors (PNETs). To date, a large number of frameshift, nonsense and missense mutations of MEN1 have been identified to be responsible for part of MEN1-defficient PNETs patients due to truncation or rapid degradation of menin protein. However, the stability of the wild-type (WT) menin in PNETs is totally unknown. In the present study, we observed ubiquitination of WT menin in 293T cells by transfection of ectopic WT menin and HA-ubiquitin. As expected, either...
Source: Bioscience Reports - October 29, 2019 Category: Biomedical Science Authors: Jiang Z, Wan S, Xing B Tags: Biosci Rep Source Type: research

A novel mass assay to measure phosphatidylinositol-5-phosphate from cells and tissues.
Abstract Phosphatidylinositol-5-phosphate (PI5P) is a low abundance lipid proposed to have functions in cell migration, DNA damage responses, receptor trafficking and insulin signalling in metazoans. However, studies of PI5P function are limited by the lack of scalable techniques to quantify its level from cells and tissues in multicellular organisms. Currently, PI5P measurement requires the use of radionuclide labelling approaches that are not easily applicable in tissues or in vivo samples. In the present study, we describe a simple and reliable, non-radioactive mass assay to measure total PI5P levels from cells...
Source: Bioscience Reports - October 29, 2019 Category: Biomedical Science Authors: Ghosh A, Sharma S, Shinde D, Ramya V, Raghu P Tags: Biosci Rep Source Type: research

BTXA regulates the epithelial-mesenchymal transition and autophagy of keloid fibroblasts via modulating miR-1587/miR-2392 targeted ZEB2.
Abstract Keloids are very resistant to treatment in dermatology and plastic surgical practice. The present study aimed to explore the underlying mechanism of botulinum toxin A (BTXA) treated human skin keloid fibroblasts (HSFBs) proving some new insights into keloids treatment. Expression of miR-1587 and miR-2392 were significantly down-regulated in keloid tissues and HSFBs, while the ZEB2 was a target of both and up-regulated in keloid tissues and HSFBs compared with the normal controls. BTXA could significantly increase the expression of miR-1587 and miR-2392 but decrease the expression of ZEB2. BTXA could signi...
Source: Bioscience Reports - October 29, 2019 Category: Biomedical Science Authors: Hou Z, Fan F, Liu P Tags: Biosci Rep Source Type: research

Identification of novel non-synonymous variants associated with type 2 diabetes-related metabolites in Korean population.
Abstract Metabolome-genome wide association studies (mGWASs) are useful for understanding the genetic regulation of metabolites in complex diseases, including type 2 diabetes (T2D). Numerous genetic variants associated with T2D-related metabolites have been identified in previous mGWASs; however, these analyses seem to have difficulty in detecting the genetic variants with functional effects. An exome array focussed on potentially functional variants is an alternative platform to obtain insight into the genetics of biochemical conversion processes. In the present study, we performed an mGWAS using 27,140 non-synon...
Source: Bioscience Reports - October 29, 2019 Category: Biomedical Science Authors: Park TJ, Lee HS, Kim YJ, Kim BJ Tags: Biosci Rep Source Type: research

Thousand and one kinase 1 protects MCAO-induced cerebral ischemic stroke in rats by decreasing apoptosis and pro-inflammatory factors.
CONCLUSION: TAOK1 protected MCAO-induced cerebral ischemic stroke by decreasing the pro-inflammatory factors and apoptosis via PI3K/AKT and MAPK signaling pathways. PMID: 31652447 [PubMed - in process] (Source: Bioscience Reports)
Source: Bioscience Reports - October 29, 2019 Category: Biomedical Science Authors: Li J, Liu Z, Wang L, Xu H, Wang Y Tags: Biosci Rep Source Type: research

Role of matrix metalloproteases 1/3 gene polymorphisms in patients with rotator cuff tear.
In conclusion, a significant correlation was identified between the MMP-1 rs1799750 polymorphism and RCT. The MMP-1 rs1799750 polymorphism might be considered as a biomarker of genetically high-risk RCT, helping to clarify the mechanism of RCT. PMID: 31652448 [PubMed - in process] (Source: Bioscience Reports)
Source: Bioscience Reports - October 29, 2019 Category: Biomedical Science Authors: Miao K, Jiang L, Zhou X, Wu L, Huang Y, Xu N, Zhang J, Li J Tags: Biosci Rep Source Type: research

Effects of the MAML2 genetic variants in glioma susceptibility and prognosis.
CONCLUSION: We provided some novel data suggesting MAML2 polymorphisms might contribute to glioma risk and prognosis. Future studies are warranted to validate these findings and characterize mechanisms underlying these associations. PMID: 31652449 [PubMed - in process] (Source: Bioscience Reports)
Source: Bioscience Reports - October 29, 2019 Category: Biomedical Science Authors: Zhang M, Zhao Y, Zhao J, Huang T, Guo X, Ma X, Wu Y Tags: Biosci Rep Source Type: research

Houshiheisan promotes angiogenesis via HIF-1 α/VEGF and SDF-1/CXCR4 pathways: in vivo and in vitro.
CONCLUSION: The present study suggests a novel application of HSHS as an effective angiogenic formula for stroke recovery. PMID: 31652450 [PubMed - in process] (Source: Bioscience Reports)
Source: Bioscience Reports - October 29, 2019 Category: Biomedical Science Authors: Xiang Y, Yao X, Wang X, Zhao H, Zou H, Wang L, Zhang QX Tags: Biosci Rep Source Type: research

Ginsenoside Rg1 attenuates isoflurane/surgery-induced cognitive disorders and sirtuin 3 dysfunction.
In conclusion, this experiment demonstrates that ginsenoside Rg1 treatment can attenuate I/S-induced neurocognitive disorders and Sirt3 dysfunction. PMID: 31652451 [PubMed - in process] (Source: Bioscience Reports)
Source: Bioscience Reports - October 29, 2019 Category: Biomedical Science Authors: Miao HH, Wang M, Wang HX, Tian M, Xue FS Tags: Biosci Rep Source Type: research

Association between PHOX2B gene rs28647582 T > C polymorphism and Wilms tumor susceptibility.
Association between PHOX2B gene rs28647582 T>C polymorphism and Wilms tumor susceptibility. Biosci Rep. 2019 Oct 30;39(10): Authors: Lin A, Fu W, Wang W, Zhu J, Liu J, Xia H, Liu G, He J Abstract Wilms tumor is one of the most common pediatric solid tumors. The pair-like homeobox 2b (PHOX2B) gene is an important transcription factor that regulates cellular proliferation and differentiation in early life. The association between PHOX2B single nucleotide polymorphisms (SNPs) and Wilms tumor risk has not been investigated. Therefore, we conducted a case-control study involving 145 Wilms tumor patients...
Source: Bioscience Reports - October 29, 2019 Category: Biomedical Science Authors: Lin A, Fu W, Wang W, Zhu J, Liu J, Xia H, Liu G, He J Tags: Biosci Rep Source Type: research

Ketamine induces endoplasmic reticulum stress in rats and SV-HUC-1 human uroepithelial cells by activating NLRP3/TXNIP aix.
Abstract Many clinical studies have been conducted on ketamine-associated cystitis. However, the underlying mechanisms of ketamine-associated cystitis still remain unclear. Bladder tissues of rats were stained by Hematoxylin and Eosin (HE). The viability of human uroepithelial cells (SV-HUC-1 cells) was determined by cell counting kit-8 (CCK-8). Apoptosis and reactive oxygen species (ROS) were examined by flow cytometry. Additionally, the expressions of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), IL-1β and IL-18 were respectively determined by reverse transcription quantitative (RTq)-PC...
Source: Bioscience Reports - October 29, 2019 Category: Biomedical Science Authors: Cui L, Jiang X, Zhang C, Li D, Yu S, Wan F, Ma Y, Guo W, Shan Z Tags: Biosci Rep Source Type: research

A novel missense variant c.G644A (p.G215E) of the RPGR gene in a Chinese family causes X-linked retinitis pigmentosa.
In conclusion, our study is the first to indicate that the novel missense variant c.G644A (p.G215E) in the RPGR gene might be the disease-causing mutation in this xlRP family, expanding mutation spectrum. These findings facilitate better understanding of the molecular pathogenesis of this disease; provide new insights for genetic counseling and healthcare. PMID: 31652454 [PubMed - in process] (Source: Bioscience Reports)
Source: Bioscience Reports - October 29, 2019 Category: Biomedical Science Authors: Fu J, Cheng J, Zhou Q, Wei C, Chen H, Lv H, Fu J Tags: Biosci Rep Source Type: research

miRNA-103 promotes chondrocyte apoptosis by down-regulation of Sphingosine kinase-1 and ameliorates PI3K/AKT pathway in osteoarthritis.
CONCLUSIONS: Our studies suggest that miRNA-103 contributes to chondrocyte apoptosis, promoting OA progression by down-regulation of PI3K/AKT pathway through the reduction in SPHK1 activity. PMID: 31652455 [PubMed - in process] (Source: Bioscience Reports)
Source: Bioscience Reports - October 29, 2019 Category: Biomedical Science Authors: Li F, Yao J, Hao Q, Duan Z Tags: Biosci Rep Source Type: research

The inhibition of tumor protein p53 by microRNA-151a-3p induced cell proliferation, migration and invasion in nasopharyngeal carcinoma.
Abstract A close relation between microRNA-151a-3p (miR-151a-3p) and nasopharyngeal carcinoma (NPC) has been reported, however, the molecular mechanism is still unclear. The aim of the present study was to explore the mechanism in the promotion of miR-151a-3p to NPC progression. The levels of miR-151-3p in several NPC cell lines were detected in order to screen an experimental cell line. MiR-151a-3p mimic and inhibitor were constructed and transfected into 5-8F cells and cell proliferation were detected by Cell Counting Kit-8 (CCK-8). The apoptosis rate, cell migration and invasion were determined by flow cytometr...
Source: Bioscience Reports - October 29, 2019 Category: Biomedical Science Authors: Liu H, Cheng Y, Xu Y, Xu H, Lin Z, Fan J, Lang J Tags: Biosci Rep Source Type: research

Commentary on: Nomograms based on inflammatory biomarkers for predicting tumor grade and microvascular invasion in stage I/II hepatocellular carcinoma.
Abstract Some doubts were generated during the reading of nomograms based on inflammatory biomarkers for preoperatively predicting tumor grade and microvascular invasion in stage I/II hepatocellular carcinoma (HCC). We would like to highlight and discuss with authors. First, neutrophil-lymphocyte ratio (NLR) and derived NLR (dNLR) should not be entered into multivariate analysis simultaneously. Second, authors should clarify how the cutoffs of these variables including lymphocyte-monocyte ratio (LMR), dNLR, age and tumor size were set. We insist that the type of variables should be consistent when we carry out the...
Source: Bioscience Reports - October 29, 2019 Category: Biomedical Science Authors: Xiao L, Zeng F, Deng G Tags: Biosci Rep Source Type: research

Retraction: Overexpressed C14orf166 associates with disease progression and poor prognosis in non-small cell lung cancer.
Authors: PMID: 31652458 [PubMed - in process] (Source: Bioscience Reports)
Source: Bioscience Reports - October 29, 2019 Category: Biomedical Science Tags: Biosci Rep Source Type: research

Role of long non-coding RNA TP73-AS1 in cancer.
Abstract Cancer incidence rate has increased so much that it is the second leading cause of deaths worldwide after cardiovascular diseases. Sensitive and specific biomarkers are needed for an early diagnosis of cancer and in-time treatment. Recent studies have found that long non-coding RNAs (lncRNAs) participate in cancer tumorigenesis. LncRNA P73 antisense RNA 1T (TP73-AS1), also known as KIAA0495 and p53-dependent apoptosis modulator (PDAM), is located in human chromosomal band 1p36.32 and plays a crucial role in many different carcinomas. This review summarizes current findings on the role of TP73-AS1 and its ...
Source: Bioscience Reports - October 29, 2019 Category: Biomedical Science Authors: Chen C, Shu L, Zou W Tags: Biosci Rep Source Type: research

Engineering anti-cancer nanovaccine based on antigen cross-presentation.
Abstract Dendritic cells (DCs) present exogenous antigens on major histocompatibility complex (MHC) class I molecules, thereby activating CD8+ T cells, contributing to tumor elimination through a mechanism known as antigen cross-presentation. A variety of factors such as maturation state of DCs, co-stimulatory signals, T-cell microenvironment, antigen internalization routes and adjuvants regulate the process of DC-mediated antigen cross-presentation. Recently, the development of successful cancer immunotherapies may be attributed to the ability of DCs to cross-present tumor antigens. In this review article, we foc...
Source: Bioscience Reports - October 29, 2019 Category: Biomedical Science Authors: Warrier VU, Makandar AI, Garg M, Sethi G, Kant R, Pal JK, Yuba E, Gupta RK Tags: Biosci Rep Source Type: research

The association of mean telomere length with all-cause, cerebrovascular and cardiovascular mortality.
Abstract Mean telomere length (MLT) is a marker of cell aging and may associate with age-related diseases. However, the relationship between MLT and mortality risk remains unclear. We aimed to investigate the relationship between MLT and all-cause, cerebrovascular and cardiovascular mortality among adults in United States. We analyzed data were from National Health and Nutrition Examination Survey (NHANES, 1999-2002) with follow-up data through 31 December 2015. Based on MLT, participants were categorized into low, middle and high groups. Multivariate Cox proportional hazards regression, subgroup analysis and gene...
Source: Bioscience Reports - October 27, 2019 Category: Biomedical Science Authors: Huang YQ, Lo K, Feng YQ, Zhang B Tags: Biosci Rep Source Type: research

Multifunctional Neuron-specific Enolase: Its Role in Lung Diseases.
This article mainly discusses the role and diagnostic potential of NSE in some lung diseases. PMID: 31642468 [PubMed - as supplied by publisher] (Source: Bioscience Reports)
Source: Bioscience Reports - October 23, 2019 Category: Biomedical Science Authors: Xu CM, Luo YL, Li S, Li ZX, Jiang L, Zhang GX, Owusu L, Chen HL Tags: Biosci Rep Source Type: research

Arih2 gene influences on immune response and tissue development in chicken.
In this study, the full-length coding sequence of Arih2 gene was cloned from Hy-Line Brown chicken. The tissue transcriptional profiles of Arih2 gene at different developmental stages were detected using qRT-PCR, and the Arih2 functional characteristics in immune response were analyzed. The results showed that the full-length coding sequence of Arih2 gene was 1473 bp, encoding 490 amino acids, and conservative between different species. The Arih2 gene was transcribed in various tissues at different developmental stages, and its transcriptional activities varied significantly between multiple tissues. With the development o...
Source: Bioscience Reports - September 24, 2019 Category: Biomedical Science Authors: Wu G, Xu S, Zhang W, Liu Y, Wang Q, Man C Tags: Biosci Rep Source Type: research

High-throughput sequencing analysis of genes encoding the B lymphocyte receptor heavy-chain CDR3 in renal and peripheral blood of IgA nephropathy.
Abstract AIM: IgA nephropathy (IgAN) is one of the most common chronic glomerulonephritis. Its etiology and pathogenesis remain unclear. We thus explored the immune repertoire of the B-cell receptor (BCR) and the heavy-chain complementarity-determining region 3 (CDR3) in renal tissue and peripheral blood of IgAN patients. METHOD: Total RNAs extracted from renal tissues and peripheral blood of patients and peripheral blood of healthy controls were analyzed via high-throughput multiplex PCR sequencing. We amplified and sequenced BCR heavy-chain CDR3 regions to explore repertoire diversity, V/J gene family distr...
Source: Bioscience Reports - September 24, 2019 Category: Biomedical Science Authors: Chen D, Zhang Z, Yang Y, Hong Q, Li W, Zhuo L Tags: Biosci Rep Source Type: research

Silencing of SPARC represses heterotopic ossification via inhibition of the MAPK signaling pathway.
This study aimed to clarify the involvement of secreted protein acidic and rich in cysteine (SPARC) and the underlying mechanism in rat model of HO. The mechanistic investigation on roles of SPARC in HO was examined through gain- and loss-of-function approaches of SPARC, with ALP activity, mineralized nodules, and OCN content measured. To further confirm the regulatory role of SPARC, levels of mitogen-activated protein kinase (MAPK) signaling pathways-related proteins (extracellular signal-regulated kinase (ERK), c-jun N-terminal kinase (JNK), p38, nuclear factor kappa-B (NF-κB), and IkB kinase β (IKKβ)) we...
Source: Bioscience Reports - September 23, 2019 Category: Biomedical Science Authors: Wang Q, Yang Q, Zhang A, Kang Z, Wang Y, Zhang Z Tags: Biosci Rep Source Type: research

A novel antimicrobial polymer efficiently treats multidrug-resistant MRSA-induced bloodstream infection.
In conclusion, all these findings presented in this work convincingly suggested that polymer 2a may be a promising therapeutic alternative for treating MRSA-induced infections, especially bloodstream infection. PMID: 31548363 [PubMed - as supplied by publisher] (Source: Bioscience Reports)
Source: Bioscience Reports - September 23, 2019 Category: Biomedical Science Authors: Chen X, Lou W, Liu J, Ding B, Fan W, Hong J Tags: Biosci Rep Source Type: research

Leucine 232 and hydrophobic residues at the ribosomal P stalk binding site are critical for biological activity of ricin.
Abstract Ricin interacts with the ribosomal P stalk to cleave a conserved adenine from the a-sarcin/ricin loop (SRL) of the rRNA. Ricin toxin A chain (RTA) uses Arg235 as the most critical arginine for binding to the P stalk through electrostatic interactions to facilitate depurination. Structural analysis showed that a P2 peptide binds to a hydrophobic pocket on RTA and the last two residues form hydrogen bonds with Arg235. The importance of hydrophobic residues relative to Arg235 in the interaction with the P stalk in vivo and on the toxicity of RTA is not known. Here, we mutated residues in the hydrophobic pock...
Source: Bioscience Reports - September 23, 2019 Category: Biomedical Science Authors: Zhou Y, Li XP, Kahn JN, McLaughlin JE, Tumer NE Tags: Biosci Rep Source Type: research

MicroRNA-367 promotes progression of hepatocellular carcinoma through PTEN /PI3K/AKT signaling pathway.
Abstract The study aimed to investigate the functional roles of micorRNA (miR)-367 in progression of hepatocellular carcinoma (HCC), as well as its regulation on PI3K/AKT pathway. The relative expression of miR-367  in HCC tissues and cell line was detected using quantitative real-time polymerase chain reaction (qRT-PCR) method. Chi-square test was applied to analyze the relationship between miR-367 expression and clinical characterizes of HCC patients. The influences of miR-367  expression on cell proliferation, migration and invasion were analyzed using MTT and transwell assays respectively. Weste...
Source: Bioscience Reports - September 20, 2019 Category: Biomedical Science Authors: Wang Z, Luo Y Tags: Biosci Rep Source Type: research