Identification of core genes and clinical roles in pregnancy-association breast cancer based on integrated analysis of different microarray profile datasets.
In conclusion, ASB6 could be a potential predictor and therapeutic target in patient with PABC. PMID: 31171715 [PubMed - as supplied by publisher] (Source: Bioscience Reports)
Source: Bioscience Reports - June 6, 2019 Category: Biomedical Science Authors: Zhang J, Zhou YJ, Yu ZH, Chen AX, Yu Y, Wang X, Cao XC Tags: Biosci Rep Source Type: research

Conformational changes in Chikungunya virus E2 protein upon heparan sulfate receptor binding explain mechanism of E2-E1 dissociation during viral entry.
Abstract Receptor-binding is the first step in viral cell entry. In enveloped virus cell entry, viral and host membrane fusion follows receptor-binding. Viral surface receptor-binding protein associates with membrane fusion protein and masks its structure, to prevent pre-mature fusion activity. Dissociation of receptor-binding protein from fusion protein is an essential step before membrane fusion. Mechanism of receptor-binding leading to dissociation of receptor-binding and fusion protein is poorly understood in alphaviruses. Chikungunya virus (CHIKV), an alphavirus, re-emerged as a global pathogen in recent past...
Source: Bioscience Reports - June 5, 2019 Category: Biomedical Science Authors: Sahoo B, Chowdary TK Tags: Biosci Rep Source Type: research

LncRNA SAMMSON negatively regulates miR-9-3p in hepatocellular carcinoma cells and has prognostic values.
Abstract In the present study we investigated the role of lncRNA SAMMSON in hepatocellular carcinoma (HCC). We found that SAMMSON was upregulated in HCC tissues, and patients with high levels of SAMMSON in HCC tissues had significantly lower overall rate within 5 years after admission. miR-9-3p was downregulated in HCC tissues and inversely correlated with SAMMSON. SAMMSON expression was not significantly affected by HBV and HCV infections in HCC patients. In HCC cells, SAMMSON overexpression resulted in downregulated miR-9-3p expression, while miR-9-3p overexpression caused no significantly changes in expression ...
Source: Bioscience Reports - June 4, 2019 Category: Biomedical Science Authors: Yang S, Cai H, Hu B, Tu J Tags: Biosci Rep Source Type: research

UGT2B17 and miR-224 contribute to hormone dependency trends in adenocarcinoma and squamous cell carcinoma of esophagus.
Abstract Esophageal squamous cell carcinoma (ESCC) and esophageal adenocarcinoma (EA) are the two main sub-types of esophageal cancer. Genetics underpinnings of EA are substantially less understood than that of ESCC. A large-scale relation data analysis was conducted to explore the genes implicated with either EA or ESCC, or both. Each gene linked to ESCC but not EA was further explored in mega-analysis of 6 independently collected EA RNA expression datasets. A multiple linear regression (MLR) model was built to study the possible influence of sample size, population region, and study date on the gene expression d...
Source: Bioscience Reports - June 4, 2019 Category: Biomedical Science Authors: Lian X, Baranova A, Ngo J, Yu G, Cao H Tags: Biosci Rep Source Type: research

Deorphanizing NUDIX hydrolases from Trypanosoma : Tantalizing links with metabolic regulation and stress tolerance.
Abstract An explosion of sequence information in the genomics era has thrown up thousands of protein sequences without functional assignment. Though our ability to predict function based on sequence alone is improving steadily, we still have a long way to go. Proteins with common evolutionary origins carry telling sequence signatures, which ought to reveal their biological roles. These sequence signatures have allowed us to classify proteins into families with similar structures and, possibly, functions. Yet, evolution is a perpetual tinkerer and hence, sequence signatures alone have proved inadequate in understan...
Source: Bioscience Reports - June 3, 2019 Category: Biomedical Science Authors: Matange N Tags: Biosci Rep Source Type: research

Association of DNAH11 gene polymorphisms with asthenozoospermia in Northeast Chinese patients.
Abstract Reduced or no progressive sperm motility in the fresh ejaculate defines asthenozoospermia as one of the major causes of male infertility. The axonemal heavy chain dynein type 11 ( DNAH11 ) gene encodes for one of the axonemal dynein heavy chain (DHC) family members and participates in assembling respiratory cilia and sperm flagella. Given the high degree of conservation of DNAH11 , mutations could give rise to primary ciliary dyskinesia (PCD) and asthenozoospermia. To date, few studies have reported on the association between variants in DNAH11 and asthenozoospermia. In the present study, 87 patients with...
Source: Bioscience Reports - June 3, 2019 Category: Biomedical Science Authors: Zhu D, Zhang H, Wang R, Liu X, Jiang Y, Feng T, Liu R, Zhang G Tags: Biosci Rep Source Type: research

MiR-505 suppressed the growth of hepatocellular carcinoma cells via targeting IGF-1R.
Abstract Hepatocellular carcinoma (HCC) is one of the most common cancers globally. An increasing body of evidence has demonstrated the critical function of microRNAs (miRNAs) in the initiation and progression of human cancers. Here, we showed that miR-505 was down-regulated in HCC tissues and cell lines. Reduced expression of miR-505 was significantly correlated with the worse prognosis of HCC patients. Overexpression of miR-505 suppressed the proliferation, colony formation and induced apoptosis of both HepG2 and Huh7 cells. Further mechanism study uncovered that miR-505 bound the 3'-untranslated region (UTR) of...
Source: Bioscience Reports - June 3, 2019 Category: Biomedical Science Authors: Ren L, Yao Y, Wang Y, Wang S Tags: Biosci Rep Source Type: research

Non-enzymatic Glucose Sensor with Electrodeposited Silver/Carbon Nanotubes Composite Electrode.
This study indicates that the Ag/CNTs electrode is possible substitute of the expensive glassy carbon electrode for enzyme free glucose sensors. PMID: 31160484 [PubMed - as supplied by publisher] (Source: Bioscience Reports)
Source: Bioscience Reports - June 3, 2019 Category: Biomedical Science Authors: Shabbir SA, Tariq S, Ashiq MGB, Khan W Tags: Biosci Rep Source Type: research

Spontaneous suprachoroidal hemorrhage in a high myopia patient with rhegmatogenous retinal detachment: A case report and literature review.
Conclusion: Advanced age, systemic anticoagulation, and hypertension are strong risk factors. RRD associated with massive SSCH is an extremely rare event. Vitrectomy and choroidal blood drainage can effectively remove suprachoroidal hemorrhage and promote retinal reattachment in these eyes. However, the final visual prognosis usually remains poor. PMID: 31160485 [PubMed - as supplied by publisher] (Source: Bioscience Reports)
Source: Bioscience Reports - June 3, 2019 Category: Biomedical Science Authors: Chai F, Zeng L, Li C, Zhao X Tags: Biosci Rep Source Type: research

Association of STAT3 and STAT4 polymorphisms with susceptibility to chronic hepatitis B virus infection and risk of hepatocellular carcinoma:a meta-analysis.
Abstract BACKGROUND: It has been reported that polymorphisms of signal transducer and activator of transcription (STAT ) 3 and STAT4 might be associated with susceptibility to hepatitis B virus (HBV) infection and risk of chronic hepatocellular carcinoma (HCC). Owing to limitation of sample size and inconclusive results, we conducted a meta-analysis to clarify the association. METHODS: We identified relevant studies by a systematic search of Medline/PubMed, EMBASE, Web of Science and the Cochrane Library through Feb.20.2019. The strength of the association measured by odds ratios (OR) with 95% confidence inte...
Source: Bioscience Reports - June 3, 2019 Category: Biomedical Science Authors: Shi H, He H, Ojha SC, Sun C, Fu J, Yan M, Deng C, Sheng YJ Tags: Biosci Rep Source Type: research

Regulatory Mechanisms of Robo4 and Their Effects on Angiogenesis.
Abstract Roundabout4 (Robo4) is a transmembrane receptor that belongs to the Roundabout (Robo) family of axon guidance molecules. Robo4 is an endothelial-specific receptor that participates in endothelial cell migration, proliferation, and angiogenesis and the maintenance of vasculature homeostasis. The purpose of this review is to summarize and analyze three main mechanisms related to the expression and function of Robo4 during developmental and pathological angiogenesis. In this review, static shear stress and the binding of transcription factors such as E26 transformation-specific variant 2 (ETV2) and Slit3 ind...
Source: Bioscience Reports - June 3, 2019 Category: Biomedical Science Authors: Dai C, Gong Q, Cheng Y, Su G Tags: Biosci Rep Source Type: research

The Emerging Role of Circular RNAs in Breast Cancer.
Abstract Breast cancer (BCa) is one of the most frequently diagnosed cancers and leading cause of cancer death among females worldwide. Circular RNAs (circRNAs) as a new class of endogenous regulatory RNAs characterized by circular shape resulting from covalently closed continuous loops that are capable of regulating gene expression at transcription or post-transcription levels. With the unique structures, circRNAs are resistant to exonuclease RNase R and maintain stability more easily than linear RNAs. Recently, an increasing number of circRNAs are discovered and reported to show different expression in BCa and t...
Source: Bioscience Reports - June 3, 2019 Category: Biomedical Science Authors: Zhou SY, Chen W, Yang SJ, Xu ZH, Hu JH, Zhang HD, Zhong SL, Tang JH Tags: Biosci Rep Source Type: research

Glypican-3 promotes cell proliferation and tumorigenesis through up-regulation of b-catenin expression in lung squamous cell carcinoma.
In conclusion, the present study demonstrates that upregulation of GPC3 accelerates the progression of lung SCC in a b-catenin-dependent manner. Our study provides a theoretical basis for GPC3/b-catenin as a novel diagnostic marker and therapeutic target for lung SCC. PMID: 31160489 [PubMed - as supplied by publisher] (Source: Bioscience Reports)
Source: Bioscience Reports - June 3, 2019 Category: Biomedical Science Authors: Wang D, Gao Y, Zhang Y, Wang L, Chen G Tags: Biosci Rep Source Type: research

Down-regulation of microRNA-138 improves immunologic function via negatively targeting p53 by regulating liver macrophage in mice with acute liver failure.
In conclusion, by negatively targeting p53, decreased miR-138 improves immunologic function by regulating liver macrophage in mouse model of ALF. PMID: 31152110 [PubMed - as supplied by publisher] (Source: Bioscience Reports)
Source: Bioscience Reports - May 31, 2019 Category: Biomedical Science Authors: Wang YQ, Lan YY, Guo YC, Yuan QW, Liu P Tags: Biosci Rep Source Type: research

High alpha B-crystallin and p53 coexpression is associated with poor prognosis in ovarian cancer.
This study investigated the correlation between alpha B-crystallin (CRYAB, HSPB5) and p53 expression in ovarian cancer and further analyzed the relationship between their expression and clinicopathology and the prognostic value of their coexpression in ovarian cancer. CRYAB and p53 expression was assessed using immunohistochemistry on ovarian cancer tumor tissues from 103 cases and validated in an independent group of 103 ovarian cancer patients. High CRYAB and p53 expression rates in ovarian cancer tissues were 61.17% and 57.28%, respectively, and their expression was positively correlated. High CRYAB and p53 coexpression...
Source: Bioscience Reports - May 31, 2019 Category: Biomedical Science Authors: Tan L, Sha L, Hou N, Zhang M, Ma Q, Shi C Tags: Biosci Rep Source Type: research

Cholesterol efflux capacity assay using immobilized liposomes and apolipoprotein B-depleted serum.
Abstract Cholesterol efflux capacity (CEC), an important functional step in reverse cholesterol transport, is the main anti-atherosclerotic function of high-density lipoprotein (HDL). Assays that improve the determination of CEC ex-vivo for clinical applications are constantly explored. In the accompanying article, Horiuchi et al, evaluate the availability of apolipoprotein B-depleted serum for CEC assays. Using their recently developed immobilized liposome-bound gel beads (ILG) method, Horiuchi et al, demonstrate that apolipoprotein B-depleted serum obtained with poly ethylene glycol precipitation enables CEC ass...
Source: Bioscience Reports - May 31, 2019 Category: Biomedical Science Authors: Sapa H Tags: Biosci Rep Source Type: research

Correction: Association between polymorphism in the promoter region of lncRNA GAS5 and the risk of colorectal cancer.
Authors: PMID: 31113845 [PubMed - in process] (Source: Bioscience Reports)
Source: Bioscience Reports - May 24, 2019 Category: Biomedical Science Tags: Biosci Rep Source Type: research

Biological enrichment prediction of polychlorinated biphenyls and novel molecular design based on 3D-QSAR/HQSAR associated with molecule docking.
Abstract Based on the experimental data of octanol-water partition coefficients (K ow, represents bioaccumulation) for 13 polychlorinated biphenyl (PCB) congeners, comparative molecular field analysis (CoMFA) and comparative molecular similarity indices analysis (CoMSIA) were used to establish 3D-QSAR models, combined with the hologram quantitative structure-activity relationship (HQSAR), the substitution sites (mono-substituted and bis-substituted) and substituent groups (electron-withdrawing hydrophobic groups) that significantly affect the octanol-water partition coefficients values of PCBs were identified, a t...
Source: Bioscience Reports - May 20, 2019 Category: Biomedical Science Authors: Yang J, Gu W, Li Y Tags: Biosci Rep Source Type: research

Correction: Discussion of relationships among changes of pathological indicators, postoperative lymphedema of the upper limb and prognosis of breast cancer patients.
Discussion of relationships among changes of pathological indicators, postoperative lymphedema of the upper limb and prognosis of breast cancer patients. Biosci Rep. 2019 May 31;39(5): Authors: PMID: 31092724 [PubMed - in process] (Source: Bioscience Reports)
Source: Bioscience Reports - May 17, 2019 Category: Biomedical Science Tags: Biosci Rep Source Type: research

Autologous activated platelet rich plasma injection into adult human ovary tissue: Molecular mechanism, analysis, and discussion of reproductive response.
Abstract In clinical infertility practice, one intractable problem is low (or absent) ovarian reserve which in turn reflects the natural oocyte depletion associated with advancing maternal age. The number of available eggs has been generally thought to be finite and strictly limited, an entrenched and largely unchallenged tenet dating back more than 50 years. In the past decade, it has been suggested that renewable ovarian germline stem cells (GSCs) exist in adults, and that such cells may be utilized as an oocyte source for women seeking to extend fertility. Currently, the issue of whether mammalian females posse...
Source: Bioscience Reports - May 15, 2019 Category: Biomedical Science Authors: Sills ES, Wood SH Tags: Biosci Rep Source Type: research

Prognostic Value of Magnetic Resonance Imaging Features in Low-Grade Gliomas.
CONCLUSION: Our study suggests that cyst formation and ring-like enhancement on pre-operative MR images can be useful to predict a favorable prognosis in patients with LGGs. PMID: 31092699 [PubMed - as supplied by publisher] (Source: Bioscience Reports)
Source: Bioscience Reports - May 15, 2019 Category: Biomedical Science Authors: Deng L, Shen L, Shen L, Zhao Z, Peng Y, Liu H, Liu H, Zhang G, Li Z, Li K, Shen E, Liu Y, Liu C, Huang X Tags: Biosci Rep Source Type: research

Long non-coding RNA SPRY4-IT1 promotes epithelial-mesenchymal transition of cervical cancer by regulating the miR-101-3p/ZEB1 axis.
CONCLUSIONS: Our findings indicate that the SPYR4-IT1/miR-101-3p /ZEB1 axis contributes to cervical cancer migration and invasion, which may provide novel insights into the function of lncRNA-driven tumorigenesis of cervical cancer. PMID: 31092700 [PubMed - as supplied by publisher] (Source: Bioscience Reports)
Source: Bioscience Reports - May 15, 2019 Category: Biomedical Science Authors: Fan MJ, Zou YZ, He PJ, Zhang S, Sun XM, Li CZ Tags: Biosci Rep Source Type: research

Short-term and long-term prognostic value of circulating soluble suppression of tumorigenicity-2 concentration in acute coronary syndrome: a meta-analysis.
We reported the prognostic value of sST2 concentration for all-cause mortality, heart failure (HF) events, and major adverse cardiovascular events (MACEs) within one month after hospitalization and during subsequent follow-up. RESULTS: Twelve studies with 11,690 ACS patients were included. Higher baseline sST2 concentration as continuous variables predict the increased risk of all-cause mortality (risk ratio [RR]: 3.16, p = 0.002), HF events (RR: 1.48, p
Source: Bioscience Reports - May 15, 2019 Category: Biomedical Science Authors: Gu L, Li J Tags: Biosci Rep Source Type: research

Identification of Key Genes Involved in Type 2 Diabetic Islet Dysfunction :a Bioinformatics Study.
Conclusion: Bioinformatics analysis revealed that ACLY and the pathways involved are possible target in the molecular mechanism of type 2 diabetes. PMID: 31088900 [PubMed - as supplied by publisher] (Source: Bioscience Reports)
Source: Bioscience Reports - May 14, 2019 Category: Biomedical Science Authors: Zhong M, Wu Y, Yang L, Ou W, Huang L Tags: Biosci Rep Source Type: research

Adiponectin and the risk of new-onset atrial fibrillation: a meta-analysis of prospective cohort studies.
Abstract BACKGROUND: Adiponectin has been suggested as a marker of many cardiovascular diseases. However, the association between serum adiponectin and incidence of atrial fibrillation (AF) in general population remains unclear. A meta-analysis was performed to systematically evaluate the potential influence of serum adiponectin at baseline on the incidence of AF during follow-up in general population. METHODS: Prospective cohort studies were identified via electronic search of PubMed and Embase databases. A randomized effect model was applied to combine the results. Predefined subgroup analyses were performe...
Source: Bioscience Reports - May 14, 2019 Category: Biomedical Science Authors: Guo Y, Liu L, Wang J Tags: Biosci Rep Source Type: research

Knockdown of Tcf3 enhances the wound healing effect of bone marrow mesenchymal stem cells in rat.
Abstract The aim of this study was to explore the wound healing effect of Tcf3 in rat BMSCs and their effects on wound healing. Epidermal growth factor (EGF) and fibroblast growth factors (FGFs) were used to induce BMSCs differentiation into epithelial-like cells. Western Blotting analysis and RT-qPCR were performed to assess the expression levels of Tcf3 and the markers of epithelial-like cells, such as Cytokeratin-18 (CK-18), CK-19 and P63. CCK-8 and clone formation assay were carried out to detect cell viability. Immunohistochemistry  and HE staining were used to assess the level of Tcf3 protein and skin r...
Source: Bioscience Reports - May 13, 2019 Category: Biomedical Science Authors: He B, Chen J, Liu L, Wang H, Wang S, Li P, Zhou J Tags: Biosci Rep Source Type: research

Long non-coding RNA MEG3 serves as a ceRNA for microRNA-145 to induce apoptosis of AC16 cardiomyocytes under high glucose condition.
In this study, we aimed to explore the potential role of long non-coding RNA MEG3 and to investigate the underlying mechanisms in human AC16 cardiomyocytes under high glucose (HG) condition. The results demonstrated that MEG3 was overexpressed in HG-treated AC16 cells, and MEG3 knockdown suppressed the HG-induced apoptosis in AC16 cells. Mechanistically, MEG3 directly binds to miR-145 in AC16 cells, thereby upregulating the expression of PDCD4. Rescue experiments showed that the role of MEG3 in HG-treated AC16 cells was partly dependent on its suppression on miR-145. In summary, our findings suggested that the role of MEG3...
Source: Bioscience Reports - May 13, 2019 Category: Biomedical Science Authors: Chen Y, Zhang Z, Zhu D, Zhao W, Li F Tags: Biosci Rep Source Type: research

miR-940 potentially promotes proliferation and metastasis of endometrial carcinoma through regulation of MRVI1.
In conclusion, our study demonstrates that miR-940 might function as a reliable diagnostic and prognostic signature in EC. PMID: 31085718 [PubMed - as supplied by publisher] (Source: Bioscience Reports)
Source: Bioscience Reports - May 13, 2019 Category: Biomedical Science Authors: Zhou Z, Xu YP, Wang LJ, Kong Y Tags: Biosci Rep Source Type: research

Evaluation of myocardial injury induced by different ablation approaches (Radiofrequency ablation versus Cryoablation) in Atrial flutter patients: A meta-analysis.
CONCLUSION: These results indicate that CRYO in comparison to RF significantly increases myocardial injury in AFL patients. Additionally, it decreases pain perception during the procedure. Further large-sampled studies are needed to support these findings. PMID: 31076543 [PubMed - as supplied by publisher] (Source: Bioscience Reports)
Source: Bioscience Reports - May 10, 2019 Category: Biomedical Science Authors: Zeng Q, Li X, Xu G Tags: Biosci Rep Source Type: research

Upregulated circular RNA VANGL1 contributes to progression of non-small cell lung cancer through inhibition of miR-195 and activation of Bcl-2.
Abstract Circular RNAs (circRNAs), a group of non-coding RNAs, play an important role in cancer biology, and in this study, we aimed to clarify the expression profiles and biological functions of circular RNA circVANGL1 in non-small cell lung cancer (NSCLC). The results showed that circVANGL1 was overexpressed in human NSCLC tissues and cell lines. circVANGL1 expression was closely associated with tumor size, TNM stage and overall survival of NSCLC patients. Further loss-of-function analysis revealed that knockdown of circVANGL1 inhibited proliferation and induced apoptosis in NSCLC cell lines. The migration and i...
Source: Bioscience Reports - May 10, 2019 Category: Biomedical Science Authors: Wang L, Ma H, Kong W, Liu B, Zhang X Tags: Biosci Rep Source Type: research

FEZF1-AS1 functions as an oncogenic lncRNA in retinoblastoma.
In conclusion, FEZF1-AS1 functions as an oncogenic lncRNA in retinoblastoma. PMID: 31076545 [PubMed - as supplied by publisher] (Source: Bioscience Reports)
Source: Bioscience Reports - May 10, 2019 Category: Biomedical Science Authors: Quan LJ, Wang WJ Tags: Biosci Rep Source Type: research

Comments on: "Association between the ICAM-1 gene polymorphism and coronary heart disease risk: a meta-analysis".
Comments on: "Association between the ICAM-1 gene polymorphism and coronary heart disease risk: a meta-analysis". Biosci Rep. 2019 May 09;: Authors: Gholami M, Amoli MM, Sharifi F Abstract Yin et al [1] recently published a meta-analysis about the association between the K469E (rs5498) polymorphism and risk of coronary heart disease (CHD). Authors included fourteen studies based on their inclusion criteria. They indicated that only studies which their genotyping data were in Hardy-Weinberg equilibrium (HWE) were included in their meta-analysis. They also tested HWE for these studies and foun...
Source: Bioscience Reports - May 9, 2019 Category: Biomedical Science Authors: Gholami M, Amoli MM, Sharifi F Tags: Biosci Rep Source Type: research

Prevention of Lipopolysaccharide-Induced CD11b+ Immune Cell Infiltration in the Kidney: Role of AT2 Receptors.
Abstract Immune cell infiltration plays a central role in mediating endotoxemic acute kidney injury (AKI). Recently, we have reported the anti-inflammatory and reno-protective role of angiotensin-II type-2 receptor (AT2R) activation under chronic low-grade inflammatory condition in the obese Zucker rat model. However, the role of AT2R activation in preventing lipopolysaccharide (LPS)-induced early infiltration of immune cells, inflammation and AKI is not known. Mice were treated with AT2R agonist C21 (0.3 mg/kg), with and without AT2R antagonist PD123319 (5 mg/kg) prior to or concurrently with LPS (5 mg/kg) challe...
Source: Bioscience Reports - May 9, 2019 Category: Biomedical Science Authors: Patel S, Dhande IS, Gray EA, Ali Q, Hussain T Tags: Biosci Rep Source Type: research

Silencing microRNA-27a inhibits proliferation and invasion of human osteosarcoma cells through the SFRP1-dependent Wnt/ β-catenin signaling pathway.
This study aims to discuss effects of miR-27a and its target gene secreted frizzled related protein 1 (SFRP1) on proliferation and invasion of human osteosarcoma cells via Wnt/β-catenin signaling pathway. The expression of miR-27a and SFRP1 in osteosarcoma tissues and cells was detected, followed by identification of their relations. Subsequently, miR-27a mimic, miR-27a inhibitor, or siRNA against SFRP1 were introduced into cells (HOS and U2OS) to investigate their role in cell proliferation and invasion. The expression of Wnt/β-catenin signaling pathway-related gene was analyzed to further uncover the regulatory...
Source: Bioscience Reports - May 9, 2019 Category: Biomedical Science Authors: Mu Y, Zhang L, Chen X, Chen S, Shi Y, Li J Tags: Biosci Rep Source Type: research

"Double-muscling" and pelvic tilt phenomena in rabbits with the cystine-knot motif deficiency of myostatin ( MSTN ) on exon 3.
In this study, CRISPR/Cas9 sgRNA anchored exon 3 of a rabbit's MSTN was used to disrupt the cystine-knot motif to change the MSTN construction and cause a loss of its function. Eleven MSTN -KO founder rabbits were generated, and all of them contained biallelic modifications. Various mutational MSTN amino acid sequences of the 11 founder rabbits were modeled to the tertiary structure using the SWISS-MODEL, and the results showed that the structure of the cystine-knot motif of each protein in the founder rabbits differed from the wild-type (WT). The MSTN -KO rabbits displayed an obvious "double-muscling" phenomena,...
Source: Bioscience Reports - May 9, 2019 Category: Biomedical Science Authors: Zhang T, Lu Y, Song S, Lu R, Zhou M, He Z, Yuan T, Yan K, Cheng Y Tags: Biosci Rep Source Type: research

Down-regulation of CCNE1 expression suppresses cell proliferation and sensitizes gastric carcinoma cells to Cisplatin.
Abstract A novel oncogene CCNE1 is considered to be associated with the development of various tumor types, its role in gastric carcinoma (GC) is little studied and the effect of CCNE1 on chemotherapy also remains unclear. We recruited 55 cases of GC tissues and corresponding normal tissues. Immunohistochemistry, quantitative real-time PCR and western blot analysis were performed to detect the expression of CCNE1. We also examined the expression of CCNE1 in gastric mucosal GES-1 cells and five GC cell lines. Silencing CCNE1 was used to assess its effect on proliferation and cell cycle in MGC-803 and NCI-N87 cells,...
Source: Bioscience Reports - May 9, 2019 Category: Biomedical Science Authors: Zhang C, Zhu Q, Gu J, Chen S, Li Q, Ying L Tags: Biosci Rep Source Type: research

Association of the TNF- α-308, TNF-α-238 Gene Polymorphisms with Risk of Bone-joint and Spinal Tuberculosis: A Meta-analysis.
Association of the TNF-α-308, TNF-α-238 Gene Polymorphisms with Risk of Bone-joint and Spinal Tuberculosis: A Meta-analysis. Biosci Rep. 2019 May 09;: Authors: Huang W, Zhou R, Li J, Wang J, Xiao H Abstract The aim of this study was to investigate the association of TNF-α-308 and TNF-α-238 gene polymorphisms with the risk of bone-joint and spinal tuberculosis by meta-analysis. By searching PubMed, Web of Science, Wanfang databases, CNKI, Medline, and Cochrane Library, the published articles about studies of the association of the TNF-α-308, TNF-α-238 gene polym...
Source: Bioscience Reports - May 9, 2019 Category: Biomedical Science Authors: Huang W, Zhou R, Li J, Wang J, Xiao H Tags: Biosci Rep Source Type: research

Ganoderic  acid A attenuates lipopolysaccharide induced lung injury in mice.
In conclusion, our study suggests that GAA attenuates acute lung injury in mouse model via the inhibition of Rho/ROCK/NF-κB pathway. PMID: 31072918 [PubMed - as supplied by publisher] (Source: Bioscience Reports)
Source: Bioscience Reports - May 9, 2019 Category: Biomedical Science Authors: Wan B, Li Y, Sun S, Yang Y, Lv Y, Wang L, Song M, Chen M, Wu C, Pan H, Zhang X Tags: Biosci Rep Source Type: research

Activin A: a novel urinary biomarker of renal impairment in multiple myeloma.
In this study, we measured urinary activin A levels in patients with newly diagnosed multiple myeloma (n=41), smoldering multiple myeloma (n=10), and monoclonal gammopathy of undetermined significance (n=28), including monoclonal gammopathy of renal significance, and assessed the correlation between urinary activin A and several clinical parameters. Urinary activin A, undetectable in healthy volunteers, was significantly increased in newly diagnosed multiple myeloma patients but not in patients with smoldering multiple myeloma and monoclonal gammopathy of undetermined significance (97.3, 25.0, and 6.61 mg/gCr, respectively, p
Source: Bioscience Reports - May 9, 2019 Category: Biomedical Science Authors: Iriuchishima H, Maeshima A, Takahashi S, Ishizaki T, Yokohama A, Tsukamoto N, Saitoh T, Murakami H, Handa H Tags: Biosci Rep Source Type: research

Oxidative stress and endoplasmic reticulum stress are involved in palmitic acid-induced H9c2 cell apoptosis.
In this study, we explored the effects of PA on proliferation and apoptosis of H9c2 cardiomyocytes, and uncovered the signaling pathways involved in PA toxicity. Our study revealed induction of both oxidative and endoplasmic reticulum (ER) stresses and exacerbation of apoptosis in PA-treated H9c2 cells. Inhibition of oxidative stress by N-acetylcysteine reduced apoptosis and decreased ER stress in PA-treated H9c2 cells. Moreover, inhibition of ER stress by 4-phenyl butyric acid decreased apoptosis and attenuated oxidative stress. In summary, this study demonstrated that oxidative stress coordinates with ER stress to play i...
Source: Bioscience Reports - May 7, 2019 Category: Biomedical Science Authors: Yang L, Guan G, Lei L, Liu J, Cao L, Wang X Tags: Biosci Rep Source Type: research

Circulating microRNA-378 levels serve as a novel biomarker for assessing  severity of coronary stenosis in patients with coronary artery disease.
CONCLUSIONS: Plasma miR-378 levels appear to be a promising non-invasive biomarker, but require to be further validated by a large cohort study in future. PMID: 31064817 [PubMed - as supplied by publisher] (Source: Bioscience Reports)
Source: Bioscience Reports - May 7, 2019 Category: Biomedical Science Authors: Li H, Gao F, Wang X, Wu J, Lu K, Liu M, Li R, Ding L, Wang R Tags: Biosci Rep Source Type: research

Colonic electrical stimulation promotes colonic motility through regeneration of myenteric plexus neurons in slow transit constipation beagles.
Abstract Slow transit constipation (STC) is a common disease characterized by markedly delayed colonic transit time as a result of colonic motility dyfunction. It is well established that STC is mostly caused by disorders of relevant nerves, especially the enteric nervous system (ENS). Colonic electrical stimulation (CES) has been regarded as a valuable alternative for the treatment of STC. However, little report focuses on the underlying nervous mechanism to normalize the delayed colonic emptying and relieve symptoms. In the present study, the therapeutic effect and the influence on ENS triggered by CES were inve...
Source: Bioscience Reports - May 7, 2019 Category: Biomedical Science Authors: Wang Y, Wang Q, Kuerban K, Dong M, Qi F, Li G, Ling J, Qiu W, Zhang W, Ye L Tags: Biosci Rep Source Type: research

Long noncoding RNA HOXA-AS2 promotes non-small cell lung cancer progression by regulating miR-520a-3p.
Conclusion: These findings implied that HOXA-AS2 promoted NSCLC progression by regulating miR-520a-3p, suggesting that HOXA-AS2 could serve as a therapeutic target for NSCLC. PMID: 31064819 [PubMed - as supplied by publisher] (Source: Bioscience Reports)
Source: Bioscience Reports - May 7, 2019 Category: Biomedical Science Authors: Liu Y, Lin X, Zhou S, Zhang P, Shao G, Yang Z Tags: Biosci Rep Source Type: research

LncRNA-H19 promotes hepatic lipogenesis by directly regulating miR-130a/PPAR γ axis in nonalcoholic fatty liver disease.
CONCLUSION:  Knockdown of H19 alleviated hepatic lipogenesis via directly regulating miR-130a/PPARγ axis, which is a novel mechanistic role of H19 in the regulation of NAFLD. PMID: 31064820 [PubMed - as supplied by publisher] (Source: Bioscience Reports)
Source: Bioscience Reports - May 7, 2019 Category: Biomedical Science Authors: Liu J, Tang T, Wang GD, Liu B Tags: Biosci Rep Source Type: research

Endoglin is a conserved regulator of vasculogenesis in zebrafish-Implications for hereditary haemorrhagic telangiectasia.
Abstract Hereditary haemorrhagic telangiectasia (HHT) is a progressive vascular disease with high mortality and prevalence. There is no effective treatment of HHT due to the lack of comprehensive knowledge of its underlying pathological mechanisms. The majority of HHT patients carry endoglin ( ENG ) mutations. Here, we used Danio rerio (zebrafish) as an in vivo model to investigate the effects of endoglin knockdown on vascular development. According to phylogenetic analyses and amino acid sequence similarity analyses, we confirmed that endoglin is conserved in vertebrates and descended from a single common ancesto...
Source: Bioscience Reports - May 7, 2019 Category: Biomedical Science Authors: Zhang D, Zhou F, Zhao X, Liu B, Chen J, Yang J Tags: Biosci Rep Source Type: research

The association between EGF A61G polymorphism and risk of colorectal cancer in a Chinese population: a case-control study.
In conclusion, EGF A61G polymorphism is a genetic contributor to CRC in a Chinese Han population. PMID: 31053624 [PubMed - as supplied by publisher] (Source: Bioscience Reports)
Source: Bioscience Reports - May 3, 2019 Category: Biomedical Science Authors: Zhu X, Shen Y, Xie Q Tags: Biosci Rep Source Type: research

Correlation between miRNA target site polymorphisms in 3' UTR of AVPR1A and risk of hypertension in Chinese Han population.
Abstract OBJECTIVE: We aimed to study the relationship between rs11174811 and rs3803107 single nucleotide polymorphisms (SNPs) in the microRNA (miRNA) target sites of the3' untranslated region (UTR) in the arginine vasopressin receptor 1a gene ( AVPR1A ) and the risk of hypertension in the Chinese Han population. METHODS: The genotypes at rs11174811 and rs3803107 were analyzed by direct sequencing in 425 Chinese Han patients with hypertension and 425 healthy subjects. AVPR1A expression was investigated by transfecting miR-526b, miR-375, and miR-186 mimics into human umbilical vein endothelial cells (HUVECs) c...
Source: Bioscience Reports - May 3, 2019 Category: Biomedical Science Authors: Zhang L, Liu J, Cheng P, Lv F Tags: Biosci Rep Source Type: research

Autologous decellularized extracellular matrix protects against H2O2-induced senescence and aging in adipose-derived stem cells and stimulates proliferation in vitro.
CONCLUSION: These findings suggest a novel approach in which an autologous decellularized extracellular matrix is used to prevent cellular senescence to enable the use of adipose-derived stem cells in regenerative medicine. PMID: 31048361 [PubMed - as supplied by publisher] (Source: Bioscience Reports)
Source: Bioscience Reports - May 2, 2019 Category: Biomedical Science Authors: Yu X, He Y, Chen Z, Qian Y, Wang J, Ji Z, Tan X, Li L, Lin M Tags: Biosci Rep Source Type: research

miR-206 inhibits cell proliferation, invasion, and migration by downregulating PTP1B in hepatocellular carcinoma.
In this study, we investigated whether miRNA participated in the regulation of PTP1B in HCC. We found that miR-206, which was downregulated during tumorigenesis, inhibited HCC cell proliferation and invasion. Overexpression of miR-206 inhibited proliferation, invasion, and migration of HCC cell lines HepG2 and Huh7. Mechanistically, we demonstrated that miR-206 directly targeted PTP1B by binding to the 3'-UTR of PTP1B mRNA as demonstrated by the luciferase reporter assay. Overexpression miR-206 inhibited PTP1B expression while miR-206 inhibition enhanced PTP1B expression in HepG2 and Huh7 cells. Functionally, the regulator...
Source: Bioscience Reports - May 2, 2019 Category: Biomedical Science Authors: Yang Q, Zhang L, Zhong Y, Lai L, Li X Tags: Biosci Rep Source Type: research

NUDIX hydrolases with inorganic polyphosphate exo- and endo-polyphosphatase activities in the glycosome, cytosol and nucleus of Trypanosoma brucei.
Abstract Trypanosoma brucei, a protist parasite that causes African trypanosomiasis or sleeping sickness, relies mainly on glycolysis for ATP production when in its mammalian host. Glycolysis occurs within a peroxisome-like organelle named the glycosome. Previous work from our laboratory reported the presence of significant amounts of inorganic polyphosphate (polyP), a polymer of three to hundreds of orthophosphate units, in the glycosomes and nucleoli of T. brucei In this work, we identified and characterized the activity of two Nudix hydrolases, TbNH2 and TbNH4, one located in the glycosomes and the other in the...
Source: Bioscience Reports - May 1, 2019 Category: Biomedical Science Authors: Cordeiro CD, Ahmed MA, Windle B, Docampo R Tags: Biosci Rep Source Type: research