Metabolic Reprogramming of the Retinal Pigment Epithelium by Cytokines Associated with Age-related Macular Degeneration
In this study, we investigate the effects of four cytokines associated with AMD, TNFα, TGF-β2, IL-6, and IL-1β, as well as a cocktail containing all four cytokines, on RPE metabolism using ARPE-19 cells, primary human RPE cells, and ex vivo rat eyecups. Strikingly, we found cytokine-specific changes in numerous metabolic markers including lactate production, glucose consumption, extracellular acidification rate, and oxygen consumption rate accompanied by increases in total mitochondrial volume and ATP production. Together, all four cytokines, could potently override the constitutive suppression of glycolysis in the RPE,...
Source: Bioscience Reports - April 3, 2024 Category: Biomedical Science Authors: David S Hansman Yuefang Ma Daniel Thomas Justine R Smith Robert J Casson Daniel Peet Source Type: research
Synergistic Action of Synthetic Peptides and Amphotericin B Causes Disruption of the Plasma Membrane and Cell Wall in Candida albicans
The objective of this work was to evaluate the combination of synthetic peptides based on the γ-core motif of defensin PvD1 with amphotericin B (AmB) at different concentrations against C. albicans. We applied the checkerboard assay using different concentrations of the commercial drug AmB and the synthetic peptides γ31-45PvD1++ and γ33-41PvD1++ against C. albicans, aiming to find combinations with synergistic interactions. Between these two interactions involving γ31-45PvD1++ and AmB, an additive effect was observed. One such interaction occurred at concentrations of 0.009 µM of peptide γ31-45PvD1++ and 13.23 µM of...
Source: Bioscience Reports - April 2, 2024 Category: Biomedical Science Authors: Thayna Souza Erica Mello Gabriel B Taveira Felipe Moreira S érgio Henrique Seabra Andr é O Carvalho Valdirene M Gomes Source Type: research
Induced pluripotent stem cells in cartilage tissue engineering: A Literature Review
CONCLUSIONS: This review has provided a comprehensive overview of these technologies and the potential applications for hiPSCs in regenerative medicine.PMID:38563479 | DOI:10.1042/BSR20232102 (Source: Bioscience Reports)
Source: Bioscience Reports - April 2, 2024 Category: Biomedical Science Authors: Amani Yousef Owaidah Source Type: research
Synergistic Action of Synthetic Peptides and Amphotericin B Causes Disruption of the Plasma Membrane and Cell Wall in Candida albicans
The objective of this work was to evaluate the combination of synthetic peptides based on the γ-core motif of defensin PvD1 with amphotericin B (AmB) at different concentrations against C. albicans. We applied the checkerboard assay using different concentrations of the commercial drug AmB and the synthetic peptides γ31-45PvD1++ and γ33-41PvD1++ against C. albicans, aiming to find combinations with synergistic interactions. Between these two interactions involving γ31-45PvD1++ and AmB, an additive effect was observed. One such interaction occurred at concentrations of 0.009 µM of peptide γ31-45PvD1++ and 13.23 µM of...
Source: Bioscience Reports - April 2, 2024 Category: Biomedical Science Authors: Thayna Souza Erica Mello Gabriel B Taveira Felipe Moreira S érgio Henrique Seabra Andr é O Carvalho Valdirene M Gomes Source Type: research
Induced pluripotent stem cells in cartilage tissue engineering: A Literature Review
CONCLUSIONS: This review has provided a comprehensive overview of these technologies and the potential applications for hiPSCs in regenerative medicine.PMID:38563479 | DOI:10.1042/BSR20232102 (Source: Bioscience Reports)
Source: Bioscience Reports - April 2, 2024 Category: Biomedical Science Authors: Amani Yousef Owaidah Source Type: research
Flow Cytometry Protocol for GLUT4-myc Detection on Cell Surfaces
Biosci Rep. 2024 Mar 27:BSR20231987. doi: 10.1042/BSR20231987. Online ahead of print.ABSTRACTInsulin and muscle contraction trigger GLUT4 translocation to the plasma membrane, which increases glucose uptake by muscle cells. Insulin resistance and type 2 diabetes are the result of impaired GLUT4 translocation. Quantifying GLUT4 translocation is essential for comprehending the intricacies of both physiological and pathophysiological processes involved in glucose metabolism. The most commonly used methods for measuring GLUT4 translocation are the ELISA-type assay and the immunofluorescence assay. While some reports suggest th...
Source: Bioscience Reports - March 27, 2024 Category: Biomedical Science Authors: Emilia Zanni-Ruiz Luis S Mayorga Martin Alejandro Pavarotti Source Type: research
Flow Cytometry Protocol for GLUT4-myc Detection on Cell Surfaces
Biosci Rep. 2024 Mar 27:BSR20231987. doi: 10.1042/BSR20231987. Online ahead of print.ABSTRACTInsulin and muscle contraction trigger GLUT4 translocation to the plasma membrane, which increases glucose uptake by muscle cells. Insulin resistance and type 2 diabetes are the result of impaired GLUT4 translocation. Quantifying GLUT4 translocation is essential for comprehending the intricacies of both physiological and pathophysiological processes involved in glucose metabolism. The most commonly used methods for measuring GLUT4 translocation are the ELISA-type assay and the immunofluorescence assay. While some reports suggest th...
Source: Bioscience Reports - March 27, 2024 Category: Biomedical Science Authors: Emilia Zanni-Ruiz Luis S Mayorga Martin Alejandro Pavarotti Source Type: research
Flow Cytometry Protocol for GLUT4-myc Detection on Cell Surfaces
Biosci Rep. 2024 Mar 27:BSR20231987. doi: 10.1042/BSR20231987. Online ahead of print.ABSTRACTInsulin and muscle contraction trigger GLUT4 translocation to the plasma membrane, which increases glucose uptake by muscle cells. Insulin resistance and type 2 diabetes are the result of impaired GLUT4 translocation. Quantifying GLUT4 translocation is essential for comprehending the intricacies of both physiological and pathophysiological processes involved in glucose metabolism. The most commonly used methods for measuring GLUT4 translocation are the ELISA-type assay and the immunofluorescence assay. While some reports suggest th...
Source: Bioscience Reports - March 27, 2024 Category: Biomedical Science Authors: Emilia Zanni-Ruiz Luis S Mayorga Martin Alejandro Pavarotti Source Type: research
Flow Cytometry Protocol for GLUT4-myc Detection on Cell Surfaces
Biosci Rep. 2024 Mar 27:BSR20231987. doi: 10.1042/BSR20231987. Online ahead of print.ABSTRACTInsulin and muscle contraction trigger GLUT4 translocation to the plasma membrane, which increases glucose uptake by muscle cells. Insulin resistance and type 2 diabetes are the result of impaired GLUT4 translocation. Quantifying GLUT4 translocation is essential for comprehending the intricacies of both physiological and pathophysiological processes involved in glucose metabolism. The most commonly used methods for measuring GLUT4 translocation are the ELISA-type assay and the immunofluorescence assay. While some reports suggest th...
Source: Bioscience Reports - March 27, 2024 Category: Biomedical Science Authors: Emilia Zanni-Ruiz Luis S Mayorga Martin Alejandro Pavarotti Source Type: research
Flow Cytometry Protocol for GLUT4-myc Detection on Cell Surfaces
Biosci Rep. 2024 Mar 27:BSR20231987. doi: 10.1042/BSR20231987. Online ahead of print.ABSTRACTInsulin and muscle contraction trigger GLUT4 translocation to the plasma membrane, which increases glucose uptake by muscle cells. Insulin resistance and type 2 diabetes are the result of impaired GLUT4 translocation. Quantifying GLUT4 translocation is essential for comprehending the intricacies of both physiological and pathophysiological processes involved in glucose metabolism. The most commonly used methods for measuring GLUT4 translocation are the ELISA-type assay and the immunofluorescence assay. While some reports suggest th...
Source: Bioscience Reports - March 27, 2024 Category: Biomedical Science Authors: Emilia Zanni-Ruiz Luis S Mayorga Martin Alejandro Pavarotti Source Type: research
Enzymatic activity of cGAS in the presence of three types of DNAs: Limited cGAS stimulation by single-stranded HIV-1 SL2 DNA
Biosci Rep. 2024 Mar 26:BSR20240269. doi: 10.1042/BSR20240269. Online ahead of print.ABSTRACTCyclic GMP-AMP (cGAMP) synthase (cGAS) is activated by binding to DNA. Activated cGAS produces 2'3'-cGAMP, which subsequently binds to the adaptor protein STING (stimulator of interferon genes). This interaction triggers the cGAS/STING signaling pathway, leading to the production of type I interferons. Three types of DNA, namely double-stranded DNA longer than 40 base pairs, a 70-nucleotide single-stranded HIV-1 DNA known as SL2, and Y-form DNA with unpaired guanosine trimers (G3 Y-form DNA), induce interferon production by activat...
Source: Bioscience Reports - March 26, 2024 Category: Biomedical Science Authors: Mineyuki Mizuguchi Niko Kyan Suzuka Nishimata Yuko Nabeshima Takayuki Obita Source Type: research
Enzymatic activity of cGAS in the presence of three types of DNAs: Limited cGAS stimulation by single-stranded HIV-1 SL2 DNA
Biosci Rep. 2024 Mar 26:BSR20240269. doi: 10.1042/BSR20240269. Online ahead of print.ABSTRACTCyclic GMP-AMP (cGAMP) synthase (cGAS) is activated by binding to DNA. Activated cGAS produces 2'3'-cGAMP, which subsequently binds to the adaptor protein STING (stimulator of interferon genes). This interaction triggers the cGAS/STING signaling pathway, leading to the production of type I interferons. Three types of DNA, namely double-stranded DNA longer than 40 base pairs, a 70-nucleotide single-stranded HIV-1 DNA known as SL2, and Y-form DNA with unpaired guanosine trimers (G3 Y-form DNA), induce interferon production by activat...
Source: Bioscience Reports - March 26, 2024 Category: Biomedical Science Authors: Mineyuki Mizuguchi Niko Kyan Suzuka Nishimata Yuko Nabeshima Takayuki Obita Source Type: research
Retraction: RASA1 inhibits the progression of renal cell carcinoma by decreasing the expression of miR-223-3p and promoting the expression of FBXW7
Biosci Rep. 2024 Mar 29;44(3):BSR-2019-4143_RET. doi: 10.1042/BSR-2019-4143_RET.NO ABSTRACTPMID:38516709 | DOI:10.1042/BSR-2019-4143_RET (Source: Bioscience Reports)
Source: Bioscience Reports - March 22, 2024 Category: Biomedical Science Source Type: research
Retraction: RASA1 inhibits the progression of renal cell carcinoma by decreasing the expression of miR-223-3p and promoting the expression of FBXW7
Biosci Rep. 2024 Mar 29;44(3):BSR-2019-4143_RET. doi: 10.1042/BSR-2019-4143_RET.NO ABSTRACTPMID:38516709 | DOI:10.1042/BSR-2019-4143_RET (Source: Bioscience Reports)
Source: Bioscience Reports - March 22, 2024 Category: Biomedical Science Source Type: research
Retraction: RASA1 inhibits the progression of renal cell carcinoma by decreasing the expression of miR-223-3p and promoting the expression of FBXW7
Biosci Rep. 2024 Mar 29;44(3):BSR-2019-4143_RET. doi: 10.1042/BSR-2019-4143_RET.NO ABSTRACTPMID:38516709 | DOI:10.1042/BSR-2019-4143_RET (Source: Bioscience Reports)
Source: Bioscience Reports - March 22, 2024 Category: Biomedical Science Source Type: research
