Identification of Structural Motifs Critical for Human G6PC2 FunctionInformed by Sequence Analysis and an AlphaFold2-Predicted Model
Biosci Rep. 2023 Dec 14:BSR20231851. doi: 10.1042/BSR20231851. Online ahead of print.ABSTRACTG6PC2 encodes a glucose-6-phosphatase (G6Pase) catalytic subunit, primarily expressed in pancreatic islet beta cells, that modulates the sensitivity of insulin secretion to glucose and thereby regulates fasting blood glucose (FBG). Mutational analyses were conducted to validate an AlphaFold2 (AF2)-predicted structure of human G6PC2 in conjunction with a novel method to solubilize and purify human G6PC2 from a heterologous expression system. These analyses show that residues forming a predicted intramolecular disulfide bond are esse...
Source: Bioscience Reports - December 14, 2023 Category: Biomedical Science Authors: Richard Mark O'Brien Emily M Hawes Derek P Claxton James K Oeser Source Type: research
Identification of Structural Motifs Critical for Human G6PC2 FunctionInformed by Sequence Analysis and an AlphaFold2-Predicted Model
Biosci Rep. 2023 Dec 14:BSR20231851. doi: 10.1042/BSR20231851. Online ahead of print.ABSTRACTG6PC2 encodes a glucose-6-phosphatase (G6Pase) catalytic subunit, primarily expressed in pancreatic islet beta cells, that modulates the sensitivity of insulin secretion to glucose and thereby regulates fasting blood glucose (FBG). Mutational analyses were conducted to validate an AlphaFold2 (AF2)-predicted structure of human G6PC2 in conjunction with a novel method to solubilize and purify human G6PC2 from a heterologous expression system. These analyses show that residues forming a predicted intramolecular disulfide bond are esse...
Source: Bioscience Reports - December 14, 2023 Category: Biomedical Science Authors: Richard Mark O'Brien Emily M Hawes Derek P Claxton James K Oeser Source Type: research
Identification of Structural Motifs Critical for Human G6PC2 FunctionInformed by Sequence Analysis and an AlphaFold2-Predicted Model
Biosci Rep. 2023 Dec 14:BSR20231851. doi: 10.1042/BSR20231851. Online ahead of print.ABSTRACTG6PC2 encodes a glucose-6-phosphatase (G6Pase) catalytic subunit, primarily expressed in pancreatic islet beta cells, that modulates the sensitivity of insulin secretion to glucose and thereby regulates fasting blood glucose (FBG). Mutational analyses were conducted to validate an AlphaFold2 (AF2)-predicted structure of human G6PC2 in conjunction with a novel method to solubilize and purify human G6PC2 from a heterologous expression system. These analyses show that residues forming a predicted intramolecular disulfide bond are esse...
Source: Bioscience Reports - December 14, 2023 Category: Biomedical Science Authors: Richard Mark O'Brien Emily M Hawes Derek P Claxton James K Oeser Source Type: research
Identification of Structural Motifs Critical for Human G6PC2 FunctionInformed by Sequence Analysis and an AlphaFold2-Predicted Model
Biosci Rep. 2023 Dec 14:BSR20231851. doi: 10.1042/BSR20231851. Online ahead of print.ABSTRACTG6PC2 encodes a glucose-6-phosphatase (G6Pase) catalytic subunit, primarily expressed in pancreatic islet beta cells, that modulates the sensitivity of insulin secretion to glucose and thereby regulates fasting blood glucose (FBG). Mutational analyses were conducted to validate an AlphaFold2 (AF2)-predicted structure of human G6PC2 in conjunction with a novel method to solubilize and purify human G6PC2 from a heterologous expression system. These analyses show that residues forming a predicted intramolecular disulfide bond are esse...
Source: Bioscience Reports - December 14, 2023 Category: Biomedical Science Authors: Richard Mark O'Brien Emily M Hawes Derek P Claxton James K Oeser Source Type: research
Potential promising anticancer applications of β-glucans: A review
Biosci Rep. 2023 Dec 13:BSR20231686. doi: 10.1042/BSR20231686. Online ahead of print.ABSTRACTβ-Glucans are valuable functional polysaccharides distributed in nature, especially in the cell walls of fungi, yeasts, bacteria, and cereals. The unique features of β-glucans, such as water solubility, viscosity, molecular weight, and so on, have rendered them to be broadly applied in various food systems as well as in medicine to improve human health. Moreover, inhibition of cancer development could be achieved by an increase in immune system activity via β-glucans. β-glucans, which are part of a class of naturally occurring ...
Source: Bioscience Reports - December 13, 2023 Category: Biomedical Science Authors: Seyed Mostafa Noorbakhsh Varnosfaderani Farnoosh Ebrahimzadeh Mahsa Akbari Oryani Saeed Khalili Faezeh Almasi Reza Mosaddeghi Heris Zahra Payandeh Mohsen Nabi Afjadi Armina Alagheband Bahrami Chen Li Source Type: research
Potential promising anticancer applications of β-glucans: A review
Biosci Rep. 2023 Dec 13:BSR20231686. doi: 10.1042/BSR20231686. Online ahead of print.ABSTRACTβ-Glucans are valuable functional polysaccharides distributed in nature, especially in the cell walls of fungi, yeasts, bacteria, and cereals. The unique features of β-glucans, such as water solubility, viscosity, molecular weight, and so on, have rendered them to be broadly applied in various food systems as well as in medicine to improve human health. Moreover, inhibition of cancer development could be achieved by an increase in immune system activity via β-glucans. β-glucans, which are part of a class of naturally occurring ...
Source: Bioscience Reports - December 13, 2023 Category: Biomedical Science Authors: Seyed Mostafa Noorbakhsh Varnosfaderani Farnoosh Ebrahimzadeh Mahsa Akbari Oryani Saeed Khalili Faezeh Almasi Reza Mosaddeghi Heris Zahra Payandeh Mohsen Nabi Afjadi Armina Alagheband Bahrami Chen Li Source Type: research
Study on the production of Sophorolipid by Starmerella bombicola yeast using waste fried oil fermentation
Biosci Rep. 2023 Dec 8:BSR20230345. doi: 10.1042/BSR20230345. Online ahead of print.ABSTRACTSophorolipid (SLs) are surface active compounds that have excellent surface-lowering properties. SLs was produced by Starmerella bombicola (CGMCC1576) yeast with sunflower seeds oil, fried waste oil, cooked tung oil and raw tung oil used as hydrophobic carbon sources. The results showed that the strain could use sunflower seed oil and fried waste oil as hydrophobic carbon sources to produce SLs, and the yields were 44.52 and 39.09 gL-1, and it could not use cooked tung oil and raw tung oil. Analysis by high performance liquid chroma...
Source: Bioscience Reports - December 8, 2023 Category: Biomedical Science Authors: Haifeng Wang Ruifang Gao Xin Song Xiangdong Yuan Xiuli Chen Yanling Zhao Source Type: research
Study on the production of Sophorolipid by Starmerella bombicola yeast using waste fried oil fermentation
Biosci Rep. 2023 Dec 8:BSR20230345. doi: 10.1042/BSR20230345. Online ahead of print.ABSTRACTSophorolipid (SLs) are surface active compounds that have excellent surface-lowering properties. SLs was produced by Starmerella bombicola (CGMCC1576) yeast with sunflower seeds oil, fried waste oil, cooked tung oil and raw tung oil used as hydrophobic carbon sources. The results showed that the strain could use sunflower seed oil and fried waste oil as hydrophobic carbon sources to produce SLs, and the yields were 44.52 and 39.09 gL-1, and it could not use cooked tung oil and raw tung oil. Analysis by high performance liquid chroma...
Source: Bioscience Reports - December 8, 2023 Category: Biomedical Science Authors: Haifeng Wang Ruifang Gao Xin Song Xiangdong Yuan Xiuli Chen Yanling Zhao Source Type: research
Study on the production of Sophorolipid by Starmerella bombicola yeast using waste fried oil fermentation
Biosci Rep. 2023 Dec 8:BSR20230345. doi: 10.1042/BSR20230345. Online ahead of print.ABSTRACTSophorolipid (SLs) are surface active compounds that have excellent surface-lowering properties. SLs was produced by Starmerella bombicola (CGMCC1576) yeast with sunflower seeds oil, fried waste oil, cooked tung oil and raw tung oil used as hydrophobic carbon sources. The results showed that the strain could use sunflower seed oil and fried waste oil as hydrophobic carbon sources to produce SLs, and the yields were 44.52 and 39.09 gL-1, and it could not use cooked tung oil and raw tung oil. Analysis by high performance liquid chroma...
Source: Bioscience Reports - December 8, 2023 Category: Biomedical Science Authors: Haifeng Wang Ruifang Gao Xin Song Xiangdong Yuan Xiuli Chen Yanling Zhao Source Type: research
Study on the production of Sophorolipid by Starmerella bombicola yeast using waste fried oil fermentation
Biosci Rep. 2023 Dec 8:BSR20230345. doi: 10.1042/BSR20230345. Online ahead of print.ABSTRACTSophorolipid (SLs) are surface active compounds that have excellent surface-lowering properties. SLs was produced by Starmerella bombicola (CGMCC1576) yeast with sunflower seeds oil, fried waste oil, cooked tung oil and raw tung oil used as hydrophobic carbon sources. The results showed that the strain could use sunflower seed oil and fried waste oil as hydrophobic carbon sources to produce SLs, and the yields were 44.52 and 39.09 gL-1, and it could not use cooked tung oil and raw tung oil. Analysis by high performance liquid chroma...
Source: Bioscience Reports - December 8, 2023 Category: Biomedical Science Authors: Haifeng Wang Ruifang Gao Xin Song Xiangdong Yuan Xiuli Chen Yanling Zhao Source Type: research
Structural and Functional Coupling in Crosslinking Uracil-DNA Glycosylase UDGX
This study offers new insight on the catalytic mechanism of UDGX and provides a unique example of enzyme evolution.PMID:38059429 | DOI:10.1042/BSR20231551 (Source: Bioscience Reports)
Source: Bioscience Reports - December 7, 2023 Category: Biomedical Science Authors: Chuan Liang Ye Yang Ping Ning Chenyan Chang Weiguo Cao Source Type: research
Structural and Functional Coupling in Crosslinking Uracil-DNA Glycosylase UDGX
This study offers new insight on the catalytic mechanism of UDGX and provides a unique example of enzyme evolution.PMID:38059429 | DOI:10.1042/BSR20231551 (Source: Bioscience Reports)
Source: Bioscience Reports - December 7, 2023 Category: Biomedical Science Authors: Chuan Liang Ye Yang Ping Ning Chenyan Chang Weiguo Cao Source Type: research
Melanogrit potentiates melanogenesis by escalating cellular tyrosinase activity and MITF levels via pERK inhibition
In this study, we investigated the therapeutic potential of herbo-mineral formulation, Melanogrit in neutralizing the white patches in the skin. The study utilized UPLC/MS-QToF technique to determine the diversified phytochemical profile in Melanogrit. The murine B16F10 cells when treated with Melanogrit underwent morphological changes, including increased angularity, enlarged cell size, and greater dendritic protrusions. To establish an equivalent model to study melanogenesis, we carefully optimized the dosage of alpha-melanocyte stimulating hormone, αMSH in B16F10 cells as an alternative to using melanocyte-keratinocyte...
Source: Bioscience Reports - December 6, 2023 Category: Biomedical Science Authors: Acharya Balkrishna Savita Lochab Sudeep Verma Jyotish Srivastava Rishabh Dev Anurag Varshney Source Type: research
Melanogrit potentiates melanogenesis by escalating cellular tyrosinase activity and MITF levels via pERK inhibition
In this study, we investigated the therapeutic potential of herbo-mineral formulation, Melanogrit in neutralizing the white patches in the skin. The study utilized UPLC/MS-QToF technique to determine the diversified phytochemical profile in Melanogrit. The murine B16F10 cells when treated with Melanogrit underwent morphological changes, including increased angularity, enlarged cell size, and greater dendritic protrusions. To establish an equivalent model to study melanogenesis, we carefully optimized the dosage of alpha-melanocyte stimulating hormone, αMSH in B16F10 cells as an alternative to using melanocyte-keratinocyte...
Source: Bioscience Reports - December 6, 2023 Category: Biomedical Science Authors: Acharya Balkrishna Savita Lochab Sudeep Verma Jyotish Srivastava Rishabh Dev Anurag Varshney Source Type: research
The differentiation courses of the Tfh cells: a new perspective on autoimmune disease pathogenesis and treatment
Biosci Rep. 2023 Dec 5:BSR20231723. doi: 10.1042/BSR20231723. Online ahead of print.ABSTRACTThe follicular helper T cells are derived from CD4+T cells, promoting the formation of germinal centers and assisting B cells to produce antibodies. This review describes the differentiation process of Tfh cells from the perspectives of the initiation, maturation, migration, efficacy, and subset classification of Tfh cells, and correlates it with autoimmune disease, to provide information for researchers to fully understand Tfh cells and provide further research ideas to manage immune-related diseases.PMID:38051200 | DOI:10.1042/BSR...
Source: Bioscience Reports - December 5, 2023 Category: Biomedical Science Authors: Qingya Yang Fang Zhang Hongyi Chen Yuman Hu Ning Yang Wenyan Yang Jing Wang Yaxu Yang Ran Xu Chao Xu Source Type: research
