Lowering oxidative stress in ghrelin cells stimulates ghrelin secretion.
Abstract Ghrelin is a predominantly stomach-derived peptide hormone with many actions including regulation of food intake, body weight, and blood glucose. Plasma ghrelin levels are robustly regulated by feeding status, with its levels increasing upon caloric restriction and decreasing after food intake. At least some of this regulation might be due to direct responsiveness of ghrelin cells to changes in circulating nutrients, including glucose. Indeed, oral and parental glucose administration to humans and mice lower plasma ghrelin. Also dissociated mouse gastric mucosal cell preparations, which contain ghrelin ce...
Source: Am J Physiol Endocri... - June 16, 2020 Category: Endocrinology Authors: Mani BK, Osborne-Lawrence S, Metzger N, Zigman JM Tags: Am J Physiol Endocrinol Metab Source Type: research

Acute insulin deprivation results in altered mitochondrial substrate sensitivity conducive to greater fatty acid transport.
Abstract Type 1 and type 2 diabetes are both tightly associated with impaired glucose control. While both pathologies stem from different mechanisms, a reduction in insulin action coincides with drastic metabolic dysfunction in skeletal muscle and metabolic inflexibility. However, the underlying explanation for this response remains poorly understood, particularly since it is difficult to distinguish the role of attenuated insulin action from the detrimental effects of reactive lipid accumulation which impair mitochondrial function and promote reactive oxygen species (ROS) emission. We therefore utilized streptozo...
Source: Am J Physiol Endocri... - June 16, 2020 Category: Endocrinology Authors: Miotto PM, Petrick HL, Holloway GP Tags: Am J Physiol Endocrinol Metab Source Type: research

Oligomeric collagen as an encapsulation material for islet/ β cell replacement: Effect of islet source, dose, implant site, and administration format.
Oligomeric collagen as an encapsulation material for islet/β cell replacement: Effect of islet source, dose, implant site, and administration format. Am J Physiol Endocrinol Metab. 2020 Jun 16;: Authors: Stephens CH, Morrison RA, McLaughlin M, Orr K, Tersey SA, Scott-Moncrieff JC, Mirmira RG, Considine RV, Voytik-Harbin S Abstract Replacement of islets/β cells that provide long-lasting glucose-sensing and insulin-releasing functions has the potential to restore extended glycemic control in individuals with type 1 diabetes. Unfortunately, persistent challenges preclude such therapies from wid...
Source: Am J Physiol Endocri... - June 16, 2020 Category: Endocrinology Authors: Stephens CH, Morrison RA, McLaughlin M, Orr K, Tersey SA, Scott-Moncrieff JC, Mirmira RG, Considine RV, Voytik-Harbin S Tags: Am J Physiol Endocrinol Metab Source Type: research

Long-term high-fat feeding exacerbates short-term increases in adipose mitochondrial reactive oxygen species, without impairing mitochondrial respiration.
Abstract White adipose tissue (WAT) dysfunction in obesity is implicated in the onset of whole-body insulin resistance. Alterations in mitochondrial bioenergetics, namely impaired mitochondrial respiration and increased mitochondrial reactive oxygen species (mtROS) production, have been suggested to contribute to this metabolic dysregulation. However, techniques investigating mitochondrial function are classically normalized to tissue weight, which may be confounding when considering obesity-related adipocyte hypertrophy. Furthermore, the effect of long-term high-fat diet (HFD) on mtROS in WAT has yet to be elucid...
Source: Am J Physiol Endocri... - June 16, 2020 Category: Endocrinology Authors: Politis-Barber V, Brunetta HS, Paglialunga S, Petrick HL, Holloway GP Tags: Am J Physiol Endocrinol Metab Source Type: research

Circulating extracellular vesicles from patients with valvular heart disease induce neutrophil chemotaxis via FOXO3a and the inhibiting role of dexmedetomidine.
Abstract We previously demonstrated that circulating extracellular vesicles(EVs) from patients with valvular heart disease(VHD, vEVs) contain inflammatory components and inhibit endothelium-dependent vasodilation. Neutrophil chemotaxis plays a key role in renal dysfunction, and dexmedetomidine (DEX) can reduce renal dysfunction in cardiac surgery. However, roles of vEVs in neutrophil chemotaxis, and effects of DEX on vEVs are unknown. Here, we investigated the impact of vEVs on neutrophil chemotaxis in kidneys and the influence of DEX on vEVs. Circulating EVs were isolated from healthy subjects and patients with V...
Source: Am J Physiol Endocri... - June 9, 2020 Category: Endocrinology Authors: Yuan HX, Chen CY, Li YQ, Ning DS, Li Y, Chen YT, Li SX, Fu MX, Li XD, Ma J, Jian YP, Liu DH, Mo ZW, Peng YM, Xu KQ, Ou ZJ, Ou JS Tags: Am J Physiol Endocrinol Metab Source Type: research

Fecal microbiota transplantation from high caloric-fed donors alters glucose metabolism in recipient mice, independently of adiposity or exercise status.
Abstract Studies suggest gut microbiota contributes to the development of obesity and the metabolic syndrome. Exercise alters microbiota composition and diversity and is protective of these maladies. We tested whether the protective metabolic effects of exercise are mediated through fecal components through assessment of body composition and metabolism in recipients of fecal microbiota transplantation (FMT) from exercise-trained (ET) mice fed normal or high energy diets. Donor C57BL/6J mice were fed a chow or high fat, high sucrose (HFHS) diet for 4wk to induce obesity and glucose intolerance. Mice were divided in...
Source: Am J Physiol Endocri... - June 9, 2020 Category: Endocrinology Authors: Zoll J, Read MR, Heywood SE, Estevez E, Marshall JP, Kammoun HL, Allen TL, Holmes AJ, Febbraio MA, Henstridge DC Tags: Am J Physiol Endocrinol Metab Source Type: research

NOD2 in hepatocytes engages a liver-gut axis to protect against steatosis, fibrosis, and gut dysbiosis during fatty liver disease in mice.
Abstract Obesity promotes non-alcoholic fatty liver disease (NAFLD). The intestinal microbiota contributes to NAFLD progression through a gut to liver pathway that promotes inflammation and fibrosis. Gut microbiota-derived factors can travel to the liver and activate immune responses in liver-resident cells to promote inflammation and NAFLD. Little is known about bacterial sensors or immune responses that can protect against NAFLD. We tested if the bacterial cell wall sensor nucleotide-binding oligomerization domain-containing (NOD)2 protects against diet-induced NAFLD in mice. Whole-body deletion of NOD2 exacerba...
Source: Am J Physiol Endocri... - June 9, 2020 Category: Endocrinology Authors: Cavallari JF, Pokrajac NT, Zlitni S, Foley KP, Henriksbo BD, Schertzer JD Tags: Am J Physiol Endocrinol Metab Source Type: research

Adipose tissue macrophage burden, systemic inflammation and insulin resistance.
Abstract Adipose tissue inflammation, as defined by macrophage accumulation, is proposed to cause insulin resistance and systemic inflammation. Because the strength of this relationship for humans is unclear, we tested whether adipose tissue macrophage (ATM) burden is correlated with these health indicators. Using immunohistochemistry we measured abdominal subcutaneous CD68+ (total ATM) , CD14+ (pro-inflammatory/M1), and CD206+ (anti-inflammatory/M2) ATM in 97 volunteers BMI 20 - 38 kg/m2, in addition to body composition, adipocyte size, HOMA-IR, ADIPOpalmitate-IR, plasma lipids, TNF and IL-6 concentrations. There...
Source: Am J Physiol Endocri... - June 2, 2020 Category: Endocrinology Authors: Jia Q, Morgan-Bathke ME, Jensen MD Tags: Am J Physiol Endocrinol Metab Source Type: research

Reply to Jakovac: About COVID-19 and vitamin D.
PMID: 32432923 [PubMed - as supplied by publisher] (Source: Am J Physiol Endocri...)
Source: Am J Physiol Endocri... - May 21, 2020 Category: Endocrinology Authors: Facchiano A, Facchiano A, Bartoli M, Ricci A, Facchiano F Tags: Am J Physiol Endocrinol Metab Source Type: research

Glycemic control of type 2 diabetic patients with coronavirus disease during hospitalization: a proposal for early insulin therapy.
PMID: 32401039 [PubMed - as supplied by publisher] (Source: Am J Physiol Endocri...)
Source: Am J Physiol Endocri... - May 14, 2020 Category: Endocrinology Authors: Nakhleh A, Shehadeh N Tags: Am J Physiol Endocrinol Metab Source Type: research

Am J Physiol Endocrinol Metab; +33 new citations
33 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Physiol Endocrinol Metab These pubmed results were generated on 2020/05/07PubMed comprises more than millions of citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites. (Source: Am J Physiol Endocri...)
Source: Am J Physiol Endocri... - May 7, 2020 Category: Endocrinology Tags: Report Source Type: research

Biological plausibility for interactions between dietary fat, resveratrol, ACE2 and SARS-CoV illness severity.
Abstract The ACE2 cellular receptor is responsible for the pathogenesis of SARS-CoV, thus impacting the entrance and clearance of the virus. Studies demonstrate that upregulation of ACE2 has a protective effect on SARS-CoV-2 illness severity. Moreover, animal studies demonstrate that dietary intake can modulate ACE2 gene expression and function. A high intake of resveratrol may have a protective role, upregulating ACE2, whereas a high intake of dietary fat may have a detrimental role, downregulating ACE2. As such, we postulate on the biological plausibility of interactions between dietary fat and/or resveratrol an...
Source: Am J Physiol Endocri... - April 20, 2020 Category: Endocrinology Authors: Horne JR, Vohl MC Tags: Am J Physiol Endocrinol Metab Source Type: research

Am J Physiol Endocrinol Metab; +23 new citations
23 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Physiol Endocrinol Metab These pubmed results were generated on 2020/04/14PubMed comprises more than millions of citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites. (Source: Am J Physiol Endocri...)
Source: Am J Physiol Endocri... - April 14, 2020 Category: Endocrinology Tags: Report Source Type: research

Alanine, arginine, cysteine, and proline but not glutamine are substrates for and acute mediators of the liver-alpha cell axis in female mice.
CONCLUSION: Our data suggest that alanine, arginine, cysteine, and proline but not glutamine are involved in the acute regulation of the liver-alpha cell axis in female mice as they all increased glucagon secretion and their disappearance rate was altered by GRA. PMID: 32255678 [PubMed - as supplied by publisher] (Source: Am J Physiol Endocri...)
Source: Am J Physiol Endocri... - April 7, 2020 Category: Endocrinology Authors: Galsgaard KD, Jepsen SL, Kjeldsen SAS, Pedersen J, Wewer Albrechtsen NJ, Holst JJ Tags: Am J Physiol Endocrinol Metab Source Type: research

Utilization of biomarkers as predictors of skeletal muscle mitochondrial content after physiological intervention and in clinical settings.
Abstract The measurement of mitochondrial content is essential for bioenergetic research as it provides a tool to evaluate if changes in mitochondrial function are strictly due to changes in content or other mechanisms that influence function. In this perspective, we argue that commonly used biomarkers of mitochondrial content may possess limited utility for capturing changes in content with physiological intervention. Moreover, we argue that they may not provide reliable estimates of content in certain pathological situations. Finally, we discuss potential solutions to overcome issues related to the utilization o...
Source: Am J Physiol Endocri... - April 7, 2020 Category: Endocrinology Authors: Groennebaek T, Nielsen J, Jespersen NR, Botker HE, de Paoli FV, Miller BF, Vissing K Tags: Am J Physiol Endocrinol Metab Source Type: research

The inducible β5i proteasome subunit contributes to proinsulin degradation in GRP94 deficient β cells and is overexpressed in type 2 diabetes pancreatic islets.
The inducible β5i proteasome subunit contributes to proinsulin degradation in GRP94 deficient β cells and is overexpressed in type 2 diabetes pancreatic islets. Am J Physiol Endocrinol Metab. 2020 Apr 07;: Authors: Khilji MS, Bresson SE, Verstappen D, Pihl C, Andersen PAK, Agergaard JB, Dahlby T, Holgersen Bryde T, Klindt K, Nielsen CK, Walentinsson A, Zivkovic D, Bousquet MP, Tyrberg B, Richardson SJ, Morgan NG, Mandrup-Poulsen T, Marzec MT Abstract Proinsulin is a misfolding-prone protein and its efficient breakdown is critical, when b-cells are confronted with high insulin biosynthetic de...
Source: Am J Physiol Endocri... - April 7, 2020 Category: Endocrinology Authors: Khilji MS, Bresson SE, Verstappen D, Pihl C, Andersen PAK, Agergaard JB, Dahlby T, Holgersen Bryde T, Klindt K, Nielsen CK, Walentinsson A, Zivkovic D, Bousquet MP, Tyrberg B, Richardson SJ, Morgan NG, Mandrup-Poulsen T, Marzec MT Tags: Am J Physiol Endocrinol Metab Source Type: research

Comparative label-free mass spectrometric analysis of temporal changes in the skeletal muscle proteome after impact trauma in rats.
This study aimed to characterize changes in the muscle proteome during the destruction, repair and early remodeling phases after impact trauma in male Wistar rats. Muscle tissue was collected from uninjured control rats and rats that were euthanized between 6 h to 14 d after impact injury. Muscle tissue was analyzed using unbiased, data-independent acquisition LC-MS/MS. We identified 770 reviewed proteins in the muscle tissue, 296 of which were differentially abundant between the control and injury groups (P£0.05). Around 50 proteins showed large differences (³10-fold) or a distinct pattern of abundance after in...
Source: Am J Physiol Endocri... - April 7, 2020 Category: Endocrinology Authors: Liu L, Broszczak DA, Broadbent JA, Singh DP, Steck R, Parker TJ, Peake JM Tags: Am J Physiol Endocrinol Metab Source Type: research

Am J Physiol Endocrinol Metab; +18 new citations
18 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Physiol Endocrinol Metab These pubmed results were generated on 2020/04/01PubMed comprises more than millions of citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites. (Source: Am J Physiol Endocri...)
Source: Am J Physiol Endocri... - April 1, 2020 Category: Endocrinology Tags: Report Source Type: research

Am J Physiol Endocrinol Metab; +26 new citations
26 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Physiol Endocrinol Metab These pubmed results were generated on 2020/03/25PubMed comprises more than millions of citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites. (Source: Am J Physiol Endocri...)
Source: Am J Physiol Endocri... - March 25, 2020 Category: Endocrinology Tags: Report Source Type: research

The effect of acute dual SGLT1/SGLT2 inhibition on incretin release and glucose metabolism after gastric bypass surgery.
bad S Abstract Enhanced meal-related enteroendocrine secretion, particularly of glucagon-like peptide-1 (GLP-1), contributes to weight-loss and improved glycemia after Roux-en-Y gastric bypass (RYGB). Dietary glucose drives GLP-1 and glucose-dependent insulinotropic polypeptide (GIP) secretion postoperatively. Understanding how glucose triggers incretin secretion following RYGB could lead to new treatments of diabetes and obesity. In vitro, incretin release depends on glucose absorption via sodium-glucose cotransporter-1 (SGLT1). We investigated the importance of SGLT1/SGLT2 for enteropancreatic hormone concentrat...
Source: Am J Physiol Endocri... - March 17, 2020 Category: Endocrinology Authors: Martinussen C, Veedfald S, Dirksen C, Bojsen-Møller KN, Svane MS, Wewer Albrechtsen NJ, van Hall G, Kristiansen VB, Fenger M, Holst JJ, Madsbad S Tags: Am J Physiol Endocrinol Metab Source Type: research

Irisin counteracts high glucose and fatty acid induced cytotoxicity by preserving AMPK-insulin receptor signaling axis in C2C12 myoblast.
In this study, we determine the effect of irisin on high glucose and fatty acid induced damages using irisin overexpressed mouse C2C12 myoblast (Irisin-C2C12) and skeletal muscle from irisin-injected mice. As compared to empty vector transfected control, irisin overexpression resulted in a marked increase of cell viability and decrease of apoptosis under high glucose stress. Progression of cell cycle into G2/M phase under proliferative condition was observed by irisin overexpression. Furthermore, glucose uptake, glycogen accumulation and phosphorylation of AMPKα / insulin receptor β subunit (IRβ) / Erk1/2 i...
Source: Am J Physiol Endocri... - March 17, 2020 Category: Endocrinology Authors: Yano N, Zhang L, Wei D, Dubielecka PM, Wei L, Zhuang S, Zhu P, Qin G, Liu PY, Chin YE, Zhao TC Tags: Am J Physiol Endocrinol Metab Source Type: research

Androgen aggravates liver fibrosis by activation of NLRP3 inflammasome in CCl4 induced liver injury mouse model.
In this study, we first investigated the differences of liver injury between male and female mice, using the CCl4 induced liver injury mouse model. It showed that the liver damage of male mice was much more severe than that of female mice. Both the acute injury and fibrosis of the liver were reduced when androgens were depleted by castration of male mice. The vulnerability of male liver was associated with testis endocrine and excessive activation of inflammatory response in the liver. Castrated male mice with testosterone supplementation showed aggravated liver inflammatory response and fibrosis. The activity of NOD-like ...
Source: Am J Physiol Endocri... - March 17, 2020 Category: Endocrinology Authors: Ma X, Zhou Y, Qiao B, Jiang S, Shen Q, Han Y, Liu A, Chen X, Wei L, Zhou L, Zhao J Tags: Am J Physiol Endocrinol Metab Source Type: research

Corrigendum.
Authors: PMID: 32150463 [PubMed - in process] (Source: Am J Physiol Endocri...)
Source: Am J Physiol Endocri... - March 1, 2020 Category: Endocrinology Tags: Am J Physiol Endocrinol Metab Source Type: research

Effects of intrauterine insulin-like growth factor-1 therapy for fetal growth restriction on adult metabolism and body composition are sex-specific.
Abstract Fetal growth restriction (FGR) is associated with compromised growth and metabolic function throughout life. Intrauterine therapy of FGR with intra-amniotic insulin-like growth factor-1 (IGF1) enhances fetal growth and alters perinatal metabolism and growth in a sex-specific manner, but the adult effects are unknown. We investigated the effects of intra-amniotic IGF1 treatment of FGR on adult growth and body composition, adrenergic sensitivity, and glucose-insulin axis regulation. Placental embolisation-induced FGR was treated with 4 weekly doses of 360 µg intra-amniotic IGF1 (FGRI) or saline (FGRS)...
Source: Am J Physiol Endocri... - February 26, 2020 Category: Endocrinology Authors: Spiroski AM, Oliver MH, Jaquiery AL, Gunn TD, Harding JE, Bloomfield FH Tags: Am J Physiol Endocrinol Metab Source Type: research

Postbiotics for NOD2 require non-hematopoietic RIPK2 to improve blood glucose and metabolic inflammation in mice.
r JD Abstract Defining the host receptors and metabolic consequences of bacterial components can help explain how the microbiome influences metabolic diseases. Bacterial peptidoglycans that activate nucleotide-binding oligomerization domain-containing (NOD)1 worsen glucose control, whereas NOD2 activation improves glycemia. Receptor-interacting serine/threonine-protein kinase 2 (RIPK2) is required for innate immunity instigated by NOD1 and NOD2. The role of RIPK2 in the divergent effects of NOD1 versus NOD2 on blood glucose was unknown. We found that whole body deletion of RIPK2 negated all effects of NOD1 or NOD2...
Source: Am J Physiol Endocri... - February 26, 2020 Category: Endocrinology Authors: Cavallari JF, Barra NG, Foley KP, Lee A, Duggan BM, Henriksbo BD, Anhê FF, Ashkar AA, Schertzer JD Tags: Am J Physiol Endocrinol Metab Source Type: research

Stat3 activation induces insulin resistance via a muscle-specific E3 ubiquitin ligase Fbxo40.
Abstract Cellular mechanisms causing insulin resistance (IR) in chronic kidney disease (CKD) are poorly understood. One potential mechanism is that CKD-induced inflammation activates the signal transducer and activator of transcription 3 (Stat3) in muscle. We uncovered increased p-Stat3 in muscles of mice with CKD or mice fed high fat diet (HFD). Activated Stat3 stimulates the expression of Fbxo40, a muscle-specific E3 ubiquitin ligase that stimulates ubiquitin conjugation leading to degradation of insulin receptor substrate 1 (IRS1). Evidence that STAT3 activates Fbxo40 includes: 1) potential Stat3 binding sites ...
Source: Am J Physiol Endocri... - February 26, 2020 Category: Endocrinology Authors: Zhang L, Chen Z, Wang Y, Tweardy DJ, Mitch WE Tags: Am J Physiol Endocrinol Metab Source Type: research

Identification and regulation of xenometabolite derivatives cis- and trans-3,4-methylene-heptanoylcarnitine in plasma and skeletal muscle of exercising humans.
t C Abstract Little is known about xenometabolites in human metabolism, particularly under exercising conditions. Previously, an exercise-modifiable, likely xenometabolite derivative, cis-3,4-methylene-heptanoylcarnitine, was reported in human plasma. Here, we identified trans-3,4-methylene-heptanoylcarnitine, and its cis-isomer, in plasma and skeletal muscle by liquid chromatography-mass spectrometry. We analyzed the regulation by exercise and the arterial-to-venous differences of these cyclopropane ring-containing carnitine esters over the hepato-splanchnic bed and the exercising leg in plasma samples obtained i...
Source: Am J Physiol Endocri... - February 26, 2020 Category: Endocrinology Authors: Sobhi HF, Zhao X, Plomgaard P, Hoene M, Hansen JS, Karus B, Niess AM, Häring HU, Lehmann R, Adams SH, Xu G, Weigert C Tags: Am J Physiol Endocrinol Metab Source Type: research

Am J Physiol Endocrinol Metab; +34 new citations
34 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Physiol Endocrinol Metab These pubmed results were generated on 2020/02/20PubMed comprises more than millions of citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites. (Source: Am J Physiol Endocri...)
Source: Am J Physiol Endocri... - February 20, 2020 Category: Endocrinology Tags: Report Source Type: research

It's well and truly time to stop stating that AMPK regulates glucose uptake and fat oxidation during exercise.
Abstract None for Perspective. PMID: 32017593 [PubMed - as supplied by publisher] (Source: Am J Physiol Endocri...)
Source: Am J Physiol Endocri... - February 4, 2020 Category: Endocrinology Authors: McConell GK Tags: Am J Physiol Endocrinol Metab Source Type: research

Combined effect of canagliflozin and exercise training on high fat diet-fed mice.
In conclusion, the combination of an SGLT2i and exercise training improves obesity, insulin resistance, and NAFLD in an additive manner. Changes of gene expression in skeletal muscle may contribute, at least in part, to the improvement of obesity and insulin sensitivity. PMID: 32017594 [PubMed - as supplied by publisher] (Source: Am J Physiol Endocri...)
Source: Am J Physiol Endocri... - February 4, 2020 Category: Endocrinology Authors: Tanaka K, Takahashi H, Katagiri S, Sasaki K, Ohsugi Y, Watanabe K, Rasadul IM, Mine K, Nagafuchi S, Iwata T, Eguchi Y, Anzai K Tags: Am J Physiol Endocrinol Metab Source Type: research

Role of gut microbiota in sex- and diet-dependent metabolic disorders that lead to early mortality of androgen receptor-deficient male mice.
Abstract The gut microbiota is involved in metabolic disorders induced by androgen deficiency after sexual maturation in males (late-onset hypogonadism). However, its role in the energy metabolism of congenital androgen deficiency (e.g., androgen insensitive syndrome) in both sexes remains elusive. Here, we examined the link between the gut microbiota and metabolic disease symptoms in androgen receptor knockout (ARKO) mouse by administering high-fat diet (HFD) and/or antibiotics. HFD-fed male, but not standard diet-fed male or HFD-fed female, ARKO mice exhibited increased feed efficiency, obesity with increased vi...
Source: Am J Physiol Endocri... - February 4, 2020 Category: Endocrinology Authors: Harada N, Hanada K, Minami Y, Kitakaze T, Ogata Y, Tokumoto H, Sato T, Kato S, Inui H, Yamaji R Tags: Am J Physiol Endocrinol Metab Source Type: research

Enoyl Coenzyme A Hydratase 1 Combats Obesity and Related Metabolic Disorders by Promoting Adipose Tissue Browning.
Abstract Browning of white adipose tissue (WAT) has been recognized as an important strategy for the treatment of obesity, insulin resistance, and diabetes. Enoyl coenzyme A hydratase 1 (ECH1) is a widely known enzyme involved in lipid metabolism. However, whether and how ECH1 is implicated in browning of WAT remains obscure. Adeno-associated virus-mediated genetic engineering of ECH1 in adipose tissue was used in investigations in mouse models of obesity induced by a high-fat diet (HFD) or browning induced by cold exposure. Metabolic parameters showed that ECH1 overexpression decreased weight gain and improved in...
Source: Am J Physiol Endocri... - January 21, 2020 Category: Endocrinology Authors: Mao X, Huang D, Rao C, Du M, Liang M, Li F, Liu B, Huang K Tags: Am J Physiol Endocrinol Metab Source Type: research

Overexpression of miR-297b-5p protects against stearic acid-induced pancreatic β-cell apoptosis by targeting LATS2.
CONCLUSIONS: Our findings indicate a suppressive role for miR-297b-5p in stearic acid-induced β-cell apoptosis, which may reveal a potential target for the treatment of β-cell dysfunction in the pathogenesis of type 2 diabetes. PMID: 31961705 [PubMed - as supplied by publisher] (Source: Am J Physiol Endocri...)
Source: Am J Physiol Endocri... - January 21, 2020 Category: Endocrinology Authors: Guo R, Yu Y, Zhang Y, Li Y, Chu X, Lu H, Sun C Tags: Am J Physiol Endocrinol Metab Source Type: research

The postnatal leptin surge is critical for the transient induction of the developmental beige adipocytes in mice.
Abstract Beige adipocytes have become a promising therapeutic target to combat obesity. Our senior author Xue previously discovered a transient but significant induction of beige adipocytes in mice during early postnatal development in mice, which peaked at postnatal day 20 (P20) and then disappeared thereafter. However, the physiological mechanism underlying the transient induction of the developmental beige cells remains mystery. Interestingly, there exists a postnatal surge of leptin in mice at postnatal day P10 prior to the appearance of the developmental beige adipocytes. Given the neurotropic effect of lepti...
Source: Am J Physiol Endocri... - January 21, 2020 Category: Endocrinology Authors: Wu R, Yu W, Fu L, Li F, Jing J, Cui X, Wang S, Cao Q, Xue B, Shi H Tags: Am J Physiol Endocrinol Metab Source Type: research

TGF- β1 promotes vitamin D-induced prostaglandin E2 synthesis by upregulating vitamin D receptor expression in human granulosa-lutein cells.
In this study, we showed that TGF-β1 significantly promoted the expression of VDR, and this stimulatory effect occurred through the ALK5 type I receptor-mediated SMAD3 and ERK1/2 signaling pathways in hGL cells. Additionally, we showed that Vit D increased the expression of COX-2 and the synthesis of PGE2 in a time- and dose-dependent manner. Furthermore, we demonstrated a synergistic effect of TGF-β1 and Vit D on the expression of COX-2 and synthesis of PGE2, and this effect could be attenuated by silencing the expression of VDR. Our findings indicate that TGF-β1 upregulates the expression of VDR, which pro...
Source: Am J Physiol Endocri... - January 21, 2020 Category: Endocrinology Authors: Wang F, Chang HM, Yi Y, Lin YM, Li H, Leung PCK Tags: Am J Physiol Endocrinol Metab Source Type: research

Upregulated Phospholipase D Activity Towards Glycosylphosphatidylinositol-Anchored Proteins in Micelle-Like Serum Complexes in Diabetic/Obese Rats and Humans.
uml;ller TD Abstract Glycosylphosphatidylinositol-anchored proteins (GPI-AP) with the complete glycolipid anchor attached are present in rat and human serum at amounts which are lower in insulin-resistant/obese rats compared to normal ones. These findings prompted further evaluation of the potential of full-length GPI-AP for the prediction and stratification of metabolically deranged states. A comparison of the signatures of horizontal surface acoustic waves which were generated by full-length GPI-AP in course of their specific capture by and subsequent dissociation from a chip-based sensor between those from rat ...
Source: Am J Physiol Endocri... - January 21, 2020 Category: Endocrinology Authors: Müller GA, Tschöp MH, Müller TD Tags: Am J Physiol Endocrinol Metab Source Type: research

Pasteurized Akkermansia muciniphila protects from fat mass gain but not from bone loss.
In conclusion, treatment with pAkk protected from ovx-induced fat mass gain but not from bone loss and reduced bone mass in gonadal intact mice. Our findings with pAkk differ from some probiotics that have been shown to protect bone mass, demonstrating that not all prebiotic and probiotic factors have the same effect on bone. PMID: 31961709 [PubMed - as supplied by publisher] (Source: Am J Physiol Endocri...)
Source: Am J Physiol Endocri... - January 21, 2020 Category: Endocrinology Authors: Lawenius L, Scheffler JM, Gustafsson KL, Henning P, Nilsson KH, Colldén H, Islander U, Plovier H, Cani PD, de Vos WM, Ohlsson C, Sjögren K Tags: Am J Physiol Endocrinol Metab Source Type: research

Supplementation of a propionate-producing consortium improves markers of insulin resistance in an in vitro model of gut-liver axis.
Abstract Gut-liver crosstalk is an important determinant of human health with profound effects on energy homeostasis. While gut microbes produce a huge range of metabolites, specific compounds such as short chain fatty acids (SCFA) can enter the portal circulation and reach the liver, a central organ involved in glucose homeostasis and diabetes control. Propionate is a major SCFA involved in activation of intestinal gluconeogenesis (IGN), thereby regulating food intake, enhancing insulin sensitivity and leading to metabolic homeostasis. Although microbiome modulating strategies may target the increased microbial p...
Source: Am J Physiol Endocri... - January 14, 2020 Category: Endocrinology Authors: El Hage R, Hernandez-Sanabria E, Calatayud Arroyo M, Van De Wiele T Tags: Am J Physiol Endocrinol Metab Source Type: research

Progesterone and estrogen regulate NALCN expression in human myometrial smooth muscle cells.
Abstract During pregnancy, the uterus transitions from a quiescent state to an excitable, highly contractile state to deliver the fetus. Two important contributors essential for this transition are hormones and ion channels, both of which modulate myometrial smooth muscle cell (MSMC) excitability. Recently, the Na+ leak channel, non-selective (NALCN) was shown to contribute to a Na+ leak current in human MSMCs, and mice lacking NALCN in the uterus had dysfunctional labor. Microarray data suggested that the pro-quiescent hormone progesterone (P4) and the pro-contractile hormone estrogen (E2) regulated this channel....
Source: Am J Physiol Endocri... - January 14, 2020 Category: Endocrinology Authors: Amazu C, Ma X, Henkes C, Ferreira JJ, Santi CM, England SK Tags: Am J Physiol Endocrinol Metab Source Type: research

Thioredoxin-interacting protein regulates glucose metabolism and improves the intracellular redox state in bovine oocytes during in vitro maturation.
Abstract The extent of glucose metabolism during oocyte maturation is closely related to oocyte developmental potential. Thioredoxin-interacting protein (TXNIP) is an α-arrestin family protein that negatively regulates glucose uptake into cells. However, little information is available regarding the function of TXNIP in bovine oocytes. Accordingly, the present study was performed to investigate the influence of TXNIP on glucose metabolism in bovine oocytes during in vitro maturation. Pharmacological inhibition of TXNIP by azaserine enhanced glucose uptake and imparted a specific metabolic effect on glycolysi...
Source: Am J Physiol Endocri... - January 14, 2020 Category: Endocrinology Authors: Jiang X, Pang Y, Zhao S, Hao H, Zhao X, Du W, Wang Y, Zhu H Tags: Am J Physiol Endocrinol Metab Source Type: research

Skeletal muscle lipid droplets are resynthesized before being coated with perilipin proteins following prolonged exercise in elite male triathletes.
rd SO Abstract Intramuscular triglycerides (IMTG) are a key substrate during prolonged exercise, but little is known about the rate of IMTG resynthesis in the post-exercise period. We investigated the hypothesis that the distribution of the lipid droplet (LD)-associated perilipin (PLIN) proteins is linked to IMTG storage following exercise. 14 elite male triathletes (27±1 y, 66.5±1.3 mL.kg-1.min-1) completed 4 h of moderate-intensity cycling. During the first 4 h of recovery, subjects received either carbohydrate or H2O, after which both groups received carbohydrate. Muscle biopsies collected pre and...
Source: Am J Physiol Endocri... - January 14, 2020 Category: Endocrinology Authors: Jevons EFP, Gejl KD, Strauss JA, Ørtenblad N, Shepherd SO Tags: Am J Physiol Endocrinol Metab Source Type: research

Measurement of bioactive osteocalcin in humans using a novel immunoassay reveals association with glucose metabolism and β-cell function.
In conclusion, measurement of ucOCN with this novel and specific assay is inversely associated with insulin resistance and β-cell dysfunction in humans. PMID: 31935114 [PubMed - as supplied by publisher] (Source: Am J Physiol Endocri...)
Source: Am J Physiol Endocri... - January 14, 2020 Category: Endocrinology Authors: Lacombe J, Al Rifai O, Loter L, Moran T, Turcotte AF, Grenier-Larouche T, Tchernof A, Biertho L, Carpentier AC, Prud'homme D, Rabasa-Lhoret R, Karsenty G, Gagnon C, Jiang W, Ferron M Tags: Am J Physiol Endocrinol Metab Source Type: research

A Low Protein Diet during Gestation and Lactation Increases Hepatic Lipid Accumulation through Autophagy and Histone Deacetylase.
Conclusion: HDAC3 regulated LC3B-induced lipid accumulation potentially through autophagic degradation of APOB and MTTP in response to amino acid limitation caused by a low protein diet. PMID: 31910026 [PubMed - as supplied by publisher] (Source: Am J Physiol Endocri...)
Source: Am J Physiol Endocri... - January 7, 2020 Category: Endocrinology Authors: Wang H, Xu GB, Hernández-Saavedra D, Chen H, Pan YX Tags: Am J Physiol Endocrinol Metab Source Type: research

Down-regulation of microRNA-873 attenuates insulin resistance and myocardial injury in rats with gestational diabetes mellitus by up-regulating IGFBP2.
Abstract Gestational diabetes mellitus (GDM) is a metabolic disorder characterized by insulin resistance, and patients with GDM have a higher risk of cardiovascular disease. Multiple microRNAs (miRNAs) are reported to be involved in the regulation of myocardial injury. Moreover, miR-873 was predicted to target insulin-like growth factor binding protein 2 (IGFBP2) through bioinformatic analysis, which was further confirmed using a luciferase assay. Thus, our objective was to assess whether microRNA-873 (miR-873) affects insulin resistance and myocardial injury in an established GDM rat model. The GDM rats were trea...
Source: Am J Physiol Endocri... - January 7, 2020 Category: Endocrinology Authors: Han N, Fang HY, Jiang JX, Xu Q Tags: Am J Physiol Endocrinol Metab Source Type: research

Effect of acute and short-term dietary fat ingestion on postprandial skeletal muscle protein synthesis rates in middle-aged, overweight and obese men.
Abstract Muscle anabolic resistance to dietary protein is associated with obesity and insulin resistance. However, the contribution of excess consumption of fat to anabolic resistance is not well studied. The aim of these studies was to test the hypothesis that acute and short-term dietary fat overload will impair the skeletal muscle protein synthetic response to dietary protein ingestion. Eight overweight/obese males [46.4±1.4 years, BMI 32.3±5.4 kg/m2] participated in the acute feeding study, which consisted of 2 randomised crossover trials. On each occasion, subjects ingested an oral meal (with an...
Source: Am J Physiol Endocri... - January 7, 2020 Category: Endocrinology Authors: Tsintzas K, Jones R, Pabla P, Mallinson J, Barrett DA, Kim DH, Cooper S, Davies A, Taylor T, Gaffney C, Chee C, van Loon LJC, Stephens FB Tags: Am J Physiol Endocrinol Metab Source Type: research

CMKLR1 deficiency attenuates androgen-inducedlipid accumulation in mice.
Abstract Excess androgen-induced obesity has become a public health problem, and its prevalence has increased substantially in recent years. Chemokine-like receptor 1 (CMKLR1), a receptor of Chemerin secreted by adipose tissue, is linked to adipocyte differentiation, adipose tissue development and obesity. However, the effect of CMKLR1 signaling on androgen-mediated adiposity in vivo remains unclear. Here, using pharmacological method, orchidectomized model and CMKLR1 knockout mice, we demonstrated that androgen excess in female mice resulted in a larger cell size in white adipose tissue (WAT) and brown adipose ti...
Source: Am J Physiol Endocri... - January 7, 2020 Category: Endocrinology Authors: Huang B, Zhao H, Huang C, Wu L, Xiang L, Wang B, Xiao T, Li M, Ren L, Niu J, Zhang J Tags: Am J Physiol Endocrinol Metab Source Type: research

Trafficking of non-esterified fatty acids in insulin resistance and relationship to dys-glycemia.
Abstract In adipose, insulin functions to suppress intracellular lipolysis and secretion of non-esterified fatty acid (NEFA) into plasma. Current tests of insulin resistance do not test this response. We applied glucose and NEFA minimal models (MM) following a frequently sampled intravenous glucose tolerance test (FSIVGTT) to assess glucose-specific and NEFA-specific insulin resistance. We used total NEFA and individual fatty acids in the NEFA MM, comparing the model parameters in metabolic syndrome (MetSyn) subjects (N=52) with optimally healthy controls (OptHC) (N=14). Results are reported as mean difference (95...
Source: Am J Physiol Endocri... - January 7, 2020 Category: Endocrinology Authors: Walker RE, Ford JL, Boston RC, Savinova OV, Harris WS, Green MH, Shearer GC Tags: Am J Physiol Endocrinol Metab Source Type: research

Am J Physiol Endocrinol Metab; +39 new citations
39 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Physiol Endocrinol Metab These pubmed results were generated on 2020/01/04PubMed comprises more than millions of citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites. (Source: Am J Physiol Endocri...)
Source: Am J Physiol Endocri... - January 4, 2020 Category: Endocrinology Tags: Report Source Type: research

Am J Physiol Endocrinol Metab; +33 new citations
33 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Physiol Endocrinol Metab These pubmed results were generated on 2019/12/19PubMed comprises more than millions of citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites. (Source: Am J Physiol Endocri...)
Source: Am J Physiol Endocri... - December 19, 2019 Category: Endocrinology Tags: Report Source Type: research

ELABELA measurements by commercial ELISA kits require sample extraction.
PMID: 31808726 [PubMed - in process] (Source: Am J Physiol Endocri...)
Source: Am J Physiol Endocri... - December 1, 2019 Category: Endocrinology Authors: Georgiadou D, Boussata S, van Dijk M Tags: Am J Physiol Endocrinol Metab Source Type: research