Serine synthesis pathway enzyme PHGDH is critical for muscle cell biomass, anabolic metabolism and mTORC1 signaling
Am J Physiol Endocrinol Metab. 2023 Nov 22. doi: 10.1152/ajpendo.00151.2023. Online ahead of print.ABSTRACTCells use glycolytic intermediates for anabolism e.g., via the serine synthesis and pentose phosphate pathways. However, we still understand poorly how these metabolic pathways contribute to skeletal muscle cell biomass generation. The first aim of this study was therefore to identify enzymes that limit protein synthesis, myotube size, and proliferation in skeletal muscle cells. We inhibited key enzymes of glycolysis, the pentose phosphate pathway, and serine synthesis pathway to evaluate their importance in C2C12 myo...
Source: Am J Physiol Endocri... - November 22, 2023 Category: Endocrinology Authors: Sakari M äntyselkä Kalle Kolari Philipp Baumert Laura Yl ä-Outinen Lauri Kuikka Suvi Lahtonen Perttu Permi Henning Wackerhage Elina Kalenius Riikka Kivel ä Juha J Hulmi Source Type: research
The effect of acute and 14-day exogenous ketone supplementation on glycaemic control in adults with type 2 diabetes: two randomized controlled trials
Am J Physiol Endocrinol Metab. 2023 Nov 22. doi: 10.1152/ajpendo.00332.2023. Online ahead of print.ABSTRACTAcute ingestion of the exogenous ketone monoester supplement [(R)-3-hydroxybutyl-(R)-3-hydroxybutyrate] lowers blood glucose, suggesting therapeutic potential in individuals with impaired glucose metabolism. However, it is unknown how acute or repeated ingestion of exogenous ketones affect blood glucose control in individuals with type 2 diabetes (T2D). We conducted two randomized, counter-balanced, double-blind, placebo-controlled crossover trials to determine if (1) acute exogenous ketone monoester (0.3 g/kg body ma...
Source: Am J Physiol Endocri... - November 22, 2023 Category: Endocrinology Authors: Kaja Falkenhain Barbara F Oliveira Hashim Islam Helena Neudorf Haoning H Cen James D Johnson Kenneth Madden Joel Singer Jeremy J Walsh Jonathan P Little Source Type: research
Content of stress granules reveals a sex difference at the early phase of cold exposure in mice
Am J Physiol Endocrinol Metab. 2023 Nov 22. doi: 10.1152/ajpendo.00317.2023. Online ahead of print.ABSTRACTAdaptive thermogenesis is a vital physiological process for small endotherms. Female animals usually are more sensitive to cold temperature due to anatomical differences. Whether there is sex difference at molecular level is unclear. Stress granules (SGs) are dynamic organelles in which untranslated mRNAs reside during cellular stress. We hypothesize that the prompt response of SGs to cold stress can reveal the molecular difference between sexes. By analyzing the content in SGs of BAT at the early phase of cold stress...
Source: Am J Physiol Endocri... - November 22, 2023 Category: Endocrinology Authors: Samson W M Cheung Jensen H C Yiu Karie T C Chin Jieling Cai Aimin Xu Chi Ming Wong Connie W Woo Source Type: research
Effects of circulating endothelial microvesicles isolated from adults with obesity on endothelial cell inflammation, apoptosis and nitric oxide production
Am J Physiol Endocrinol Metab. 2023 Nov 22. doi: 10.1152/ajpendo.00139.2023. Online ahead of print.ABSTRACTCirculating endothelial-cell derived microvesicles (EMVs) have been shown to be elevated with obesity and associated with endothelial dysfunction; however, their direct effect on endothelial cells is unknown. The experimental aim of this study was to determine the effect of EMVs isolated from adults with obesity on endothelial cell inflammation, apoptosis, and nitric oxide (NO) production. EMVs (CD144+ microvesicles) were identified, enumerated and isolated from plasma by flow cytometry from 24 sedentary adults: 12 no...
Source: Am J Physiol Endocri... - November 22, 2023 Category: Endocrinology Authors: Vinicius P Garcia Hannah K Fandl Jamie G Hijmans Auburn R Berry Hannah L Cardenas Kelly A Stockelman Noah M DeSouza John W Treuth Jared J Greiner Andrew Joon Park Brian L Stauffer Christopher A DeSouza Source Type: research
Serine synthesis pathway enzyme PHGDH is critical for muscle cell biomass, anabolic metabolism and mTORC1 signaling
Am J Physiol Endocrinol Metab. 2023 Nov 22. doi: 10.1152/ajpendo.00151.2023. Online ahead of print.ABSTRACTCells use glycolytic intermediates for anabolism e.g., via the serine synthesis and pentose phosphate pathways. However, we still understand poorly how these metabolic pathways contribute to skeletal muscle cell biomass generation. The first aim of this study was therefore to identify enzymes that limit protein synthesis, myotube size, and proliferation in skeletal muscle cells. We inhibited key enzymes of glycolysis, the pentose phosphate pathway, and serine synthesis pathway to evaluate their importance in C2C12 myo...
Source: Am J Physiol Endocri... - November 22, 2023 Category: Endocrinology Authors: Sakari M äntyselkä Kalle Kolari Philipp Baumert Laura Yl ä-Outinen Lauri Kuikka Suvi Lahtonen Perttu Permi Henning Wackerhage Elina Kalenius Riikka Kivel ä Juha J Hulmi Source Type: research
