Cationic gas-filled microbubbles for ultrasound-based nucleic acids delivery.

Cationic gas-filled microbubbles for ultrasound-based nucleic acids delivery. Biosci Rep. 2017 Nov 27;: Authors: Delalande A, Bastié C, Pigeon L, Manta S, Lebertre M, Mignet N, Midoux P, Pichon C Abstract The use of ultrasound has gained great interest for nucleic acids delivery. Ultrasound can reach deep tissues in non-invasive manner. The process of sonoporation is based on the use of low frequency ultrasound combined with gas-filled microbubbles allowing an improved delivery of molecules including nucleic acids in the insonified tissue. For in vivo gene transfer, the engineering of cationic microbubbles is essential for creating strong electrostatic interactions between microbubbles and nucleic acids leading to their protection against nucleases degradation and high concentration within the target tissue. Cationic microbubbles must be stable enough to withstand nucleic acids interaction, have a good size distribution for in vivo administration, and enough acoustic activity to be detected by echography. This review aims to summarize the basic principles of ultrasound-based delivery and new knowledge acquired these recent years about this method. A focus is made on gene delivery by discussing reported studies made with cationic microbubbles including ours. They have the ability to efficient delivery of plasmid DNA, mRNA or siRNA. Last, we discuss about the key challenges that have to be faced for a fine use of this delivery system....
Source: Bioscience Reports - Category: Biomedical Science Authors: Tags: Biosci Rep Source Type: research